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Merge pull request #125 from nicost/Multi-MDA
Added MultiMDA
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autogenerated: true | ||
title: Multi-MDA | ||
redirect_from: /wiki/Multi-MDA | ||
layout: page | ||
section: Extend | ||
--- | ||
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The Multi-MDA plugin makes it possible to run time-lapse acquisitions | ||
with different acquisition settings at different positions. This can | ||
be useful - for instance - when imaging cells expressing different | ||
constructs in multiple wells of a plate, or to compare imaging in | ||
confocal and wide-field modes. | ||
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The plugin can be reached under Plugins > Acquisition Tools > Multi MDA, | ||
or using the Multi MDA button in the Multi-D Acq. window. | ||
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The UI look as follows: | ||
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![](media/Multi-MDA.png) | ||
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Use "Number of different settings" to set how many different settings | ||
you would like to use withing this Multi-MDA. For each of these | ||
settings, you will be prompted for an Acquisition Settings file that | ||
you should generate in the Multi-D Acquisition Window. Use the "Save" | ||
button in that dialog to save the settings that you then can load here. | ||
You most likely will also want to load a Position List File (generate | ||
one using the "Stage Position List" Dialog. OPtionally, you can check | ||
the "Use Preset" checkbox in the top row of the dialog and select a | ||
PresetGroup. You can then select a preset for each setting, and that | ||
preset will be applied at each time point, before each setting is run. | ||
You could, for instance, use this to switch between Confocal and Epi | ||
imaging modes. | ||
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When you make changes to one of the files, press the "Reload Settings" | ||
button to make sure the new version of the file will be used (and to | ||
update the summary/explanation line). |
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