Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Add Genome and Proteomics labs #167

Merged
merged 20 commits into from
Oct 3, 2024
Merged
Show file tree
Hide file tree
Changes from all commits
Commits
File filter

Filter by extension

Filter by extension

Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
Binary file added communities/all/labs/assets/site-switcher.png
Loading
Sorry, something went wrong. Reload?
Sorry, we cannot display this file.
Sorry, this file is invalid so it cannot be displayed.
1 change: 1 addition & 0 deletions communities/all/labs/genome
1 change: 1 addition & 0 deletions communities/all/labs/proteomics
1 change: 1 addition & 0 deletions communities/all/labs/single-cell
1 change: 1 addition & 0 deletions communities/all/labs/spoc
3 changes: 3 additions & 0 deletions communities/genome/lab/CONTRIBUTORS
Original file line number Diff line number Diff line change
@@ -0,0 +1,3 @@
# If GitHub username, name and avatar will be fetched and displayed
AnnaSyme
neoformit
361 changes: 361 additions & 0 deletions communities/genome/lab/annotation.yml

Large diffs are not rendered by default.

375 changes: 375 additions & 0 deletions communities/genome/lab/assembly.yml

Large diffs are not rendered by default.

33 changes: 33 additions & 0 deletions communities/genome/lab/base.yml
Original file line number Diff line number Diff line change
@@ -0,0 +1,33 @@
# Request this on site.usegalaxy.org.au with:
# https://site.usegalaxy.org.au/lab/export?content_root=https://github.com/galaxyproject/galaxy_codex/blob/main/subdomains/genome/base.yml

# Check out the documentation for building exported labs:
# https://site.usegalaxy.org.au/lab/export

# Use these variables in HTML templates like:
# "Welcome to the Galaxy {{ site_name }} {{ lab_name }}"
# To make the content more generic and reusable across sites
site_name: ""
lab_name: Genome Lab
nationality: ""
galaxy_base_url: https://genome.usegalaxy.org # Use for rendering tool/workflow URLs. Trailing '/' will be removed.
subdomain: genome
root_domain: usegalaxy.org
# feedback_email: [email protected] # set to enable feedback form

# Custom content relative to this file URL
header_logo: static/logo.png
custom_css: static/custom.css
intro_md: templates/intro.html
conclusion_md: templates/conclusion.html
footer_md: templates/footer.html


# Data (Tools, Workflows etc.) to be rendered into sections/tabs/accordion elements.
# Either:
# 1. Relative to this file URL
# 2. Full URL to fetch content from a different remote location
sections:
- data.yml
- assembly.yml
- annotation.yml
164 changes: 164 additions & 0 deletions communities/genome/lab/data.yml
Original file line number Diff line number Diff line change
@@ -0,0 +1,164 @@
id: data
title: Data import and preparation
tabs:
- id: help
title: Overview
heading_md: >
If you are new to galaxy, uploading your data is a good place to start!
<br>
Check out the Tools and Workflows tabs for different approaches to uploading data.
content:
- title_md: How can I import my genomics data?
description_md: >
<p>
You can upload your data to Galaxy using the Upload tool from anywhere in Galaxy. Just look for the "Upload data" button at the top of the tool panel.
</p>
button_md: More info
button_link: https://training.galaxyproject.org/training-material/topics/galaxy-interface/
- title_md: How can I subsample my data?
description_md: >
<p>
We recommend subsampling large data sets to test tools and workflows. A useful tool is <code>seqtk_seq</code>, setting the parameter at "Sample fraction of sequences".
</p>
- title_md: How can I import data from the BPA portal?
description_md: >
<p>
BioPlatforms Australia allows data downloads via URL. Once you have generated one of these URLs in the BPA portal, you can import it into Galaxy using the "Fetch data" feature of the Upload tool.
</p>
button_md: More info
button_link: https://australianbiocommons.github.io/how-to-guides/genome_assembly/hifi_assembly#in-depth-workflow-guide
- title_md: Can I upload sensitive data?
description_md: >
<p>
No, do not upload personal or sensitive, such as human health or clinical data. Please see our <a href="/about#data-privacy">Data Privacy</a> page for definitions of sensitive and health-related information.
</p>
<p>
Please also make sure you have read our <a href="/about#terms-of-service">Terms of Service</a>, which covers hosting and analysis of research data.
</p>
- title_md: Is my data private?
description_md: >
<p>
Please read our <a href="/about#data-privacy">Privacy Policy</a> for information on your personal data and any data that you upload.
</p>
- title_md: How can I increase my storage quota?
description_md: >
<p>
Please submit a quota request if your Galaxy Australia account reaches its data storage limit. Requests are usually provisioned quickly if you provide a reasonable use case for your request.
</p>
button_md: Request
button_link: /request/quota
- title_md: "Tutorial: Quality Control"
description_md: >
<p>
Quality control and data cleaning is an essential first step in any NGS analysis. This tutorial will show you how to use and interpret results from <code>FastQC</code>, <code>NanoPlot</code> and <code>PycoQC</code>.
</p>
button_md: Tutorial
button_link: https://training.galaxyproject.org/training-material/topics/sequence-analysis/tutorials/quality-control/tutorial.html
- title_md: "Tutorial: introduction to Genomics and Galaxy"
description_md: >
<p>
This practical aims to familiarize you with the Galaxy user interface. It will teach you how to perform basic tasks such as importing data, running tools, working with histories, creating workflows, and sharing your work.
</p>
button_md: Tutorial
button_link: https://training.galaxyproject.org/training-material/topics/introduction/tutorials/galaxy-intro-strands/tutorial.html
- title_md: Galaxy Australia support
description_md: >
<p>
Any user of Galaxy Australia can request support through an online form.
</p>
button_md: Request support
button_link: /request/support
- id: tools
title: Tools
heading_md: >
Common tools are listed here, or search for more in the full tool panel to the left.
content:
- title_md: Import data to Galaxy
description_md: >
Standard upload of data to Galaxy, from your computer or from the web.
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=upload1"
- title_md: <code>FastQC</code> - sequence quality reports
description_md: >
<p>
Before using your sequencing data, it's important to ensure that
the data quality is sufficient for your analysis.
</p>
inputs:
- datatypes:
- fastq
- bam
- sam
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Ffastqc%2Ffastqc"
- title_md: <code>FastP</code> - sequence quality reports, trimming & filtering
description_md: >
<p>
Faster run than FastQC, this tool can also trim reads and filter by quality.
</p>
inputs:
- datatypes:
- fastq
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Ffastp%2Ffastp"
- title_md: <code>NanoPlot</code> - visualize Oxford Nanopore data
description_md: >
<p>
A plotting suite for Oxford Nanopore sequencing data and alignments.
</p>
inputs:
- datatypes:
- fastq
- fasta
- vcf_bgzip
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fnanoplot%2Fnanoplot"
- title_md: <code>GenomeScope</code> - estimate genome size
description_md: >
<p>
A set of metrics and graphs to visualize genome size and complexity prior to assembly.
</p>
inputs:
- datatypes:
- tabular
label: Output from <code>Meryl</code> or <code>Jellyfish histo</code>
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fgenomescope%2Fgenomescope"
- title_md: <code>Meryl</code> - count kmers
description_md: >
<p>
Prepare kmer count histogram for input to GenomeScope.
</p>
inputs:
- datatypes:
- fastq
- fasta
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fmeryl%2Fmeryl"
- id: workflows
title: Workflows
heading_md: >
A workflow is a series of Galaxy tools that have been linked together to perform a specific analysis. You can use and customize the example workflows below.
<a href="https://galaxyproject.org/learn/advanced-workflow/" target="_blank">Learn more.</a>
content:
- title_md: Data QC
description_md: >
<p>
Report statistics from sequencing reads. <br> <br> Tools: <code>nanoplot</code> <code>fastqc</code> <code>multiqc</code>
</p>
button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=222"
view_link: https://workflowhub.eu/workflows/222
view_tip: View in WorkflowHub
button_tip: Import to Galaxy AU
- title_md: Kmer counting to estimate genome size
description_md: >
<p>
Estimates genome size and heterozygosity based on counts of kmers. <br> <br> Tools: <code>meryl</code> <code>genomescope</code>
</p>
button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=223"
view_link: https://workflowhub.eu/workflows/223
view_tip: View in WorkflowHub
button_tip: Import to Galaxy AU
- title_md: Trim and filter reads
description_md: >
<p>
Trims and filters raw sequence reads according to specified settings. <br> <br> Tools: <code>fastp</code>
</p>
button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=224"
view_link: https://workflowhub.eu/workflows/224
view_tip: View in WorkflowHub
button_tip: Import to Galaxy AU
Loading
Sorry, something went wrong. Reload?
Sorry, we cannot display this file.
Sorry, this file is invalid so it cannot be displayed.
Loading
Sorry, something went wrong. Reload?
Sorry, we cannot display this file.
Sorry, this file is invalid so it cannot be displayed.
Binary file added communities/genome/lab/static/logo.png
Loading
Sorry, something went wrong. Reload?
Sorry, we cannot display this file.
Sorry, this file is invalid so it cannot be displayed.
Empty file.
40 changes: 40 additions & 0 deletions communities/genome/lab/templates/intro.html
Original file line number Diff line number Diff line change
@@ -0,0 +1,40 @@
<section class="my-5" id="tagline">
<p>
Welcome to the Galaxy {{ site_name }} {{ lab_name }}. Get quick access to tools, workflows and tutorials for genome assembly and annotation.
<br>
<a
href="#"
data-bs-toggle="modal"
data-bs-target="#infoModal">
What is this page?
</a>
</p>

<div class="modal fade" id="infoModal" tabindex="-1">
<div class="modal-dialog modal-lg">
<div class="modal-content">
<div class="modal-header">
<h5 class="modal-title">Galaxy {{ site_name }}</h5>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<p>
This site <em>{{ subdomain }}.{{ root_domain }}</em> is connected to the same server as <em>{{ root_domain }}</em>, but with an interface dedicated to helping our {{ nationality }} researchers. Your history, jobs and data quota are shared with the "Base" website.
</p>

<div class="my-3">
Switch between sites quickly using the dropdown in Galaxy {{ site_name }}'s main navigation bar (note, if your Galaxy server does not have this, please request that they install the "Site Switcher" plugin from Galaxy Australia):
<img class="w-100 my-3 mx-auto" src="https://github.com/galaxyproject/galaxy-codex/blob/main/labs/assets/site-switcher.png?raw=true" alt="Switch site button">
</div>

<p><a class="ga-btn" href="{{ galaxy_base_url }}" target="_blank">Take me back to Galaxy {{ site_name }}</a></p>
<p><a class="ga-btn" href="/about">What is Galaxy {{ site_name }}?</a></p>
<p><a class="ga-btn" href="/request/support">Galaxy {{ site_name }} support</a></p>
</div>
<div class="modal-footer">
<button type="button" class="btn btn-secondary" data-bs-dismiss="modal">Close</button>
</div>
</div>
</div>
</div>
</section>
22 changes: 22 additions & 0 deletions communities/genome/lab/usegalaxy.org.au.yml
Original file line number Diff line number Diff line change
@@ -0,0 +1,22 @@
# This content extends base.yml

# Request this on site.usegalaxy.org.au with:
# https://site.usegalaxy.org.au/lab/export?content_root=https://github.com/galaxyproject/galaxy_codex/blob/main/subdomains/genome/usegalaxy.org.au.yml

# Check out the documentation for building exported labs:
# https://site.usegalaxy.org.au/lab/export

# Use these variables in HTML templates like:
# "Welcome to the Galaxy {{ site_name }} {{ lab_name }}"
# To make the content more generic and reusable across sites
site_name: Australia
lab_name: Genome Lab
nationality: Australian
galaxy_base_url: https://genome.usegalaxy.org.au # Use for rendering tool/workflow URLs. Trailing '/' will be removed.
subdomain: genome
root_domain: usegalaxy.org.au
feedback_email: [email protected]

# Custom content relative to this file URL
conclusion_md: usegalaxy.org.au/templates/conclusion.html
footer_md: usegalaxy.org.au/templates/footer.html
Loading