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simoncchu/SpliceJumper
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Usage: ./SpliceJumper [options] Required options: -P/G indicates collecting features / indicates output results -r FILE reference file(indexed) -b/i FILE input bam/prediction file(index and sorted) -o FILE output file name -l INT read length Optimal options -c INT slack value for split position with default 3 -m DOUBLE mean insert size -v DOUBLE standard variation of insert size 1.Feature collection: ./SpliceJumper -P -r ./human_g1k_v37.fasta -b ./simulated_data_input.bam -o test_sim.txt -l 100 -c 5 2. Training and prediction 2.1 Training python easy.py training_data 2.2 Predicting ..\windows\svm-predict data.scale Trained.model predictResult 3. Output generated splicing junctions ./SpliceJumper -G -i prediction.txt -o result.txt
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A tool for splicing junction calling from RNA-Seq data
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