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Update project.md
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nekrut authored Mar 22, 2024
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# Analysis project

## Project groups

I have subdivided this class into the following groups:

| Group | MEGA plate starting point | Figure in the paper's [supplement](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5534434/bin/NIHMS874162-supplement-Supplemental_Methods_And_Figures.pdf) |
Expand All @@ -10,4 +12,20 @@ I have subdivided this class into the following groups:
| 4 | Manifold 2 | S3 B |
| 5 | Manifold 5 | S3 B |

These numbers correspond to colony labels in
These numbers correspond to colony labels in Fig S3 os the [supplement](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5534434/bin/NIHMS874162-supplement-Supplemental_Methods_And_Figures.pdf).

## Task 1
(due March 26, 2024 in class)

1. Go to NCBI SRA
2. Find datasets corresponding to `SRP077287`
3. Download RunInfo table as I showed in class
4. Look at figure S3A (if you are `Sym`) or figure S3B (if you are any of the `Manifold`s)
5. Find a colony corresponding to your number (for `Sym` you can pick any trajectory you like)
6. Write down numbers of colonies corresponding to a progression so that you have a good representatioin of colonies starting from wild type to super-resistant (see image below for an example)
7. Pull rows corresponding to these numbers from RunInfo table (Step 3)
8. Upload this into Galaxy
9. La Fin



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