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dmr multi will not create necessary directories #294

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Yang990-sys opened this issue Nov 5, 2024 · 7 comments
Open

dmr multi will not create necessary directories #294

Yang990-sys opened this issue Nov 5, 2024 · 7 comments
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bug Something isn't working troubleshooting workflow and data preparation questions

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@Yang990-sys
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Yang990-sys commented Nov 5, 2024

After following your guidance in #143 , we have made the following attempts:
image

while, all regions failed for the following reason:
image

In order to check for file errors, I gave different sample names to four samples, and the results were normal:
image

Is there any way to solve my problem?

@Yang990-sys
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my modkit version :
image

@Yang990-sys
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Yang990-sys commented Nov 5, 2024

I found the problem. The problem is that the output folder must exist, otherwise an error will occur after creating the folder.

@Yang990-sys Yang990-sys reopened this Nov 5, 2024
@ArtRand
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ArtRand commented Nov 6, 2024

Hello @Yang990-sys,

Thanks for finding this. I think modkit dmr should make the folder if it doesn't exist to align it's behavior with the rest of the commands. I'll make sure to add this to the next release.

@ArtRand ArtRand added the bug Something isn't working label Nov 6, 2024
@ArtRand
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ArtRand commented Nov 11, 2024

I'm going to keep this open to track the work. Thanks again for noticing the inconsistency.

@ArtRand ArtRand reopened this Nov 11, 2024
@ArtRand ArtRand changed the title bedMethyl index missing in dmr multi with replicates dmr multi will not create necessary directories Nov 13, 2024
@Yang990-sys
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The reason I found seems inaccurate because I encountered this error again. Looking forward to software updates

@ArtRand
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ArtRand commented Nov 25, 2024

Hello @Yang990-sys,

Could you attach the log file for the command that is failing to this thread? Also could you run

tabix -l ${sample}

for each of the samples you have and attach that as well?

@ArtRand ArtRand added the troubleshooting workflow and data preparation questions label Nov 25, 2024
@Yang990-sys
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Because there is no problem running for a single sample, it should not be an issue with the index itself. When there is a duplicate, the first step of the program is to merge the duplicate samples. So I guess it may be because the merged file cannot use the original index?

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Labels
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