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communities/microgalaxy/lab/sections/1_data_import_and_preparation.yml
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id: data | ||
title: Data import and preparation | ||
tabs: | ||
- id: tools | ||
title: Tools | ||
heading_md: > | ||
Common tools are listed here, or search for more in the full tool panel to the left. | ||
content: | ||
- title_md: Import data to Galaxy | ||
description_md: > | ||
Standard upload of data to Galaxy, from your computer or from the web. | ||
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=upload1" | ||
- title_md: <code>FastQC</code> - sequence quality reports | ||
description_md: > | ||
<p> | ||
Before using your sequencing data, it's important to ensure that | ||
the data quality is sufficient for your analysis. | ||
</p> | ||
inputs: | ||
- datatypes: | ||
- fastq | ||
- bam | ||
- sam | ||
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Ffastqc%2Ffastqc" | ||
- title_md: <code>FastP</code> - sequence quality reports, trimming & filtering | ||
description_md: > | ||
<p> | ||
Faster run than FastQC, this tool can also trim reads and filter by quality. | ||
</p> | ||
inputs: | ||
- datatypes: | ||
- fastq | ||
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Ffastp%2Ffastp" | ||
- title_md: <code>NanoPlot</code> - visualize Oxford Nanopore data | ||
description_md: > | ||
<p> | ||
A plotting suite for Oxford Nanopore sequencing data and alignments. | ||
</p> | ||
inputs: | ||
- datatypes: | ||
- fastq | ||
- fasta | ||
- vcf_bgzip | ||
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fnanoplot%2Fnanoplot" | ||
- title_md: <code>GenomeScope</code> - estimate genome size | ||
description_md: > | ||
<p> | ||
A set of metrics and graphs to visualize genome size and complexity prior to assembly. | ||
</p> | ||
inputs: | ||
- datatypes: | ||
- tabular | ||
label: Output from <code>Meryl</code> or <code>Jellyfish histo</code> | ||
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fgenomescope%2Fgenomescope" | ||
- title_md: <code>Meryl</code> - count kmers | ||
description_md: > | ||
<p> | ||
Prepare kmer count histogram for input to GenomeScope. | ||
</p> | ||
inputs: | ||
- datatypes: | ||
- fastq | ||
- fasta | ||
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fmeryl%2Fmeryl" | ||
|
||
- id: workflows | ||
title: Workflows | ||
heading_md: > | ||
A workflow is a series of Galaxy tools that have been linked together to perform a specific analysis. You can use and customize the example workflows below. | ||
<a href="https://galaxyproject.org/learn/advanced-workflow/" target="_blank">Learn more.</a> | ||
content: | ||
- title_md: Data QC | ||
description_md: > | ||
<p> | ||
Report statistics from sequencing reads. <br> <br> Tools: <code>nanoplot</code> <code>fastqc</code> <code>multiqc</code> | ||
</p> | ||
button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=222" | ||
view_link: https://workflowhub.eu/workflows/222 | ||
view_tip: View in WorkflowHub | ||
button_tip: Import to Galaxy AU | ||
- title_md: Kmer counting to estimate genome size | ||
description_md: > | ||
<p> | ||
Estimates genome size and heterozygosity based on counts of kmers. <br> <br> Tools: <code>meryl</code> <code>genomescope</code> | ||
</p> | ||
button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=223" | ||
view_link: https://workflowhub.eu/workflows/223 | ||
view_tip: View in WorkflowHub | ||
button_tip: Import to Galaxy AU | ||
- title_md: Trim and filter reads | ||
description_md: > | ||
<p> | ||
Trims and filters raw sequence reads according to specified settings. <br> <br> Tools: <code>fastp</code> | ||
</p> | ||
button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=224" | ||
view_link: https://workflowhub.eu/workflows/224 | ||
view_tip: View in WorkflowHub | ||
button_tip: Import to Galaxy AU | ||
|
||
- id: tutorials | ||
title: Tutorials | ||
heading_md: > | ||
content: [] | ||
|
||
- id: faq | ||
title: FAQ | ||
heading_md: > | ||
content: [] |
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