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mr-c edited this page Aug 26, 2013 · 4 revisions

2012-paper-diginorm

khmer bits

  • sandbox/load-into-hashbits.py
  • scripts/load-into-counting.py
  • scripts/normalize-by-median.py
  • scripts/filter-abund.py
  • scripts/count-overlap.py
  • scripts/count-median.py

external bits

  • bowtie-build
  • samtools {faidx,view,sort,index}
  • bowtie

internal bits

  • calc-r2.py
  • make-random-genome.py
  • make-random-transcriptome.py
  • make-reads.py
  • make-biased-reads.py
  • read-kmer-rank-dist.py
  • find-missing-kmers.py
  • mapping-genome-cov-3.py
  • compare-map-med.py
  • mapping-cov-3.py

2013-khmer-counting

khmer bits

  • scripts/abundance-dist-single.py
  • scripts/load-into-counting.py
  • sandbox/hi-lo-abundance-by-position.py
  • scripts/filter-abund.py
  • scripts/normalize-by-median.py
  • scripts/filter-abund-single.py
  • sandbox/strip-and-split-for-assembly.py

internal bits

  • khmer-count-kmers.py
  • jelly-search.sh
  • tallymer-search.sh
  • get_random_kmers_1M_C3.py
  • simulate_random_reads.py
  • fp_assessment.py
  • count-singleton-kmers-at-end.py
  • extract-filter-abund-rounds.py
  • ngs-scripts/blast/calc-blast-cover.py

external bits

  • jellyfish
  • gt {suffixerator,tallymer}
  • dsk
  • blast: formatdb, blastall

"eel-pond" mrnaseq

3rd party

  • trimmomatic
  • libgtextutils
  • fastx
  • trinity
  • bowtie
  • RSEM package: rsem-{prepare-reference,calculate-expression}
  • blast/formatdb

khmer bits

  • scripts/normalize-by-median.py
  • filter-abund.py
  • scripts/do-partition.py

custom bits

  • eel-pond/rename-with-partitions.py
  • eel-pond/make-uni-best-hits.py
  • eel-pond/make-reciprocal-best-hits.py
  • eel-pond/make-namedb.py
  • screed.fadbm (source?)
  • eel-pond/annotate-seqs.py

The Kalamazoo Metagenome Assembly Tutorial

https://github.com/ged-lab/khmer-protocols/blob/master/metagenomics/

third-party

  • Trimmomatic
  • libgtextutils
  • fastx
  • gunzip
  • velvet
  • IDBA
  • bowtie

khmer bits

  • scripts/normalize-by-median.py
  • scripts/filter-abund.py
  • scripts/extract-paired-reads.py
  • sandbox/readstats.py
  • scripts/do-partition.py
  • scripts/extract-partitions.py
  • sandbox/sweep-reads3.py
  • sandbox/assemstats3.py
  • sandbox/extract-long-sequences.py
  • sandbox/make-coverage.py

STAMPS example

https://github.com/ged-lab/khmer/blob/master/examples/stamps/do.sh

khmer bits

  • scripts/load-into-counting.py
  • scripts/abundance-dist.py
  • scripts/normalize-by-median.py
  • scripts/do-partition.py
  • sandbox/error-correct-pass2.py
  • scripts/extract-partitions.py
  • scripts/filter-abund.py