Tutorials for the crisprVerse

Getting started

1. Installation
2. Building a genome index for off-target alignment
3. Specifying a custom nuclease
4. Building a gene annotation object

Common gRNA design workflows

5. CRISPR knockout (CRISPRko) design with Cas9
6. CRISPR knockout (CRISPRko) design with Cas12a
7. CRISPR knockdown (CRISPRkd) design with Cas13d
8. CRISPR knockdown (CRISPRkd) design with the Csm complex
9. CRISPR base editing (CRISPRbe) design with BE4max
10. CRISPR activation (CRISPRa) design
11. CRISPR interference (CRISPRi) design

Less common gRNA design workflows

12. Paired gRNA design
13. Working with custom DNA sequences
14. Design for optical pooled screening (OPS)

Miscellaneous

15. Mapping gRNAs across species
16. Working with minor and major alleles
17. Validating existing gRNA libraries
18. Building a gRNA database across all protein-coding genes