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LaraFuhrmann committed Sep 25, 2024
2 parents 6494b86 + 79bb431 commit e8b6da7
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2 changes: 1 addition & 1 deletion config/config.yaml
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@@ -1,4 +1,4 @@
fname_reference: resources/NC_045512.2.fasta
fname_insert_bed: resources/SARS-CoV-2.v532.insert.bed
fname_insert_bed: resources/SARS-CoV-2.v4.insert.bed
fname_samples: config/samples.csv
dir_path_samples: # add path to samples here
13 changes: 12 additions & 1 deletion resources/setup_emergence_new_variant/README.md
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@@ -1 +1,12 @@
This is a workflow that uses the parent workflow to process a specific set of samples to compute their divesity.
This workflow produces Figure 6 in the Manuscript of VILOCA.

To reproduce the results and figure:
1.) Clone the repository.

2.) Move into this directory: `cd resources/setup_emergence_new_variant`

3.) Install conda enviroments needed for the the workflow:`snakemake --conda-create-envs-only --use-conda -c1 --rerun-incomplete`

4.) Execute workflow. On slurm cluster the script `run_workflow.sh` can be used.

5.) When you received the two needed output files `results/all_cooccurring_mutations.csv` and `results/samples.all_coverage.csv`, figure can be generated with the notebook `workflow/notebooks/rise_of_ba.5.ipynb`
2 changes: 1 addition & 1 deletion resources/setup_emergence_new_variant/config/config.yaml
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@@ -1,4 +1,4 @@
fname_reference: ../NC_045512.2.fasta
fname_insert_bed: ../SARS-CoV-2.insert.bed
fname_insert_bed: resources/SARS-CoV-2.insert.reduced.250bp.bed
fname_samples: config/samples.csv
dir_path_samples: /cluster/project/pangolin/work-vp-test/results/
199 changes: 0 additions & 199 deletions resources/setup_emergence_new_variant/config/samples.csv
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@@ -1,259 +1,60 @@
,sample,batch
0,A2_10_2022_04_07,20220422_HTN5VDRXY
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124,E2_10_2022_09_19,20221003_HJK2VDRX2
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132,F2_10_2022_05_10,20220520_HTYK7DRXY
133,F2_10_2022_05_17,20220530_HTYKCDRXY
134,F2_10_2022_05_24,20220603_H7WH5DRX2
135,F2_10_2022_06_07,20220617_H7FWCDRX2
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138,F2_10_2022_06_28,20220708_o28874
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140,F2_10_2022_07_12,20220722_H7GYWDRX2
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142,F2_10_2022_08_02,20220812_H7CKVDRX2
143,F2_10_2022_08_09,20220819_HHMMYDRX2
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145,F2_10_2022_08_23,20220902_HGTHMDRX2
146,F2_10_2022_08_30,20220909_HHM33DRX2
147,F2_10_2022_09_06,20220916_HJK3KDRX2
148,F2_10_2022_09_13,20220923_HJ5H7DRX2
149,F2_10_2022_09_20,20221003_HJK2VDRX2
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151,G1_10_2022_05_26,20220610_H7W3KDRX2
152,H1_10_2022_04_06,20220422_HTN5VDRXY
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162,H1_10_2022_06_15,20220701_H32LGDRX2
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165,H1_10_2022_07_06,20220722_H7GYWDRX2
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167,H1_10_2022_07_27,20220812_H7CKVDRX2
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169,H1_10_2022_08_10,20220826_HHVTYDRX2
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171,H1_10_2022_08_24,20220909_HHM33DRX2
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177,A2_10_2022_10_06,20221021_HJ3NNDRX2
178,A2_10_2022_10_13,20221028_HL53FDRX2
179,A2_10_2022_10_27,20221111_HL3KJDRX2
180,B2_10_2022_10_07,20221021_HJ3NNDRX2
181,B2_10_2022_10_14,20221028_HL53FDRX2
182,B2_10_2022_10_28,20221111_HL3KJDRX2
183,C2_10_2022_10_01,20221018_HJ3MWDRX2
184,C2_10_2022_10_08,20221021_HJ3NNDRX2
185,C2_10_2022_10_15,20221028_HL53FDRX2
186,C2_10_2022_10_29,20221111_HL3KJDRX2
187,D2_10_2022_10_02,20221018_HJ3MWDRX2
188,D2_10_2022_10_09,20221021_HJ3NNDRX2
189,D2_10_2022_10_16,20221028_HL53FDRX2
190,D2_10_2022_10_30,20221111_HL3KJDRX2
191,E2_10_2022_10_03,20221018_HJ3MWDRX2
192,E2_10_2022_10_10,20221021_HJ3NNDRX2
193,E2_10_2022_10_17,20221028_HL53FDRX2
194,E2_10_2022_10_31,20221111_HL3KJDRX2
195,F2_10_2022_10_04,20221018_HJ3MWDRX2
196,F2_10_2022_10_11,20221021_HJ3NNDRX2
197,F2_10_2022_10_18,20221028_HL53FDRX2
198,H1_10_2022_10_05,20221021_HJ3NNDRX2
199,H1_10_2022_10_12,20221028_HL53FDRX2
200,H1_10_2022_10_26,20221111_HL3KJDRX2
201,A2_10_2022_11_03,20221117_HL3GFDRX2
202,A2_10_2022_11_10,20221125_HM23VDRX2
203,A2_10_2022_11_17,20221205_HM2L5DRX2
204,A2_10_2022_11_24,20221209_HM2NWDRX2
205,B2_10_2022_11_04,20221117_HL3GFDRX2
206,B2_10_2022_11_11,20221125_HM23VDRX2
207,B2_10_2022_11_18,20221205_HM2L5DRX2
208,B2_10_2022_11_25,20221209_HM2NWDRX2
209,C2_10_2022_11_05,20221117_HL3GFDRX2
210,C2_10_2022_11_12,20221125_HM23VDRX2
211,C2_10_2022_11_19,20221205_HM2L5DRX2
212,C2_10_2022_11_26,20221209_HM2NWDRX2
213,D2_10_2022_11_06,20221117_HL3GFDRX2
214,D2_10_2022_11_13,20221125_HM23VDRX2
215,D2_10_2022_11_20,20221205_HM2L5DRX2
216,D2_10_2022_11_27,20221209_HM2NWDRX2
217,D3_10_2022_11_15,20221125_HM23VDRX2
218,E2_10_2022_11_07,20221117_HL3GFDRX2
219,E2_10_2022_11_14,20221125_HM23VDRX2
220,E2_10_2022_11_21,20221205_HM2L5DRX2
221,E2_10_2022_11_28,20221209_HM2NWDRX2
222,F2_10_2022_11_01,20221111_HL3KJDRX2
223,F2_10_2022_11_08,20221117_HL3GFDRX2
224,F2_10_2022_11_22,20221205_HM2L5DRX2
225,F2_10_2022_11_29,20221209_HM2NWDRX2
226,H1_10_2022_11_02,20221117_HL3GFDRX2
227,H1_10_2022_11_09,20221125_HM23VDRX2
228,H1_10_2022_11_16,20221205_HM2L5DRX2
229,H1_10_2022_11_23,20221209_HM2NWDRX2
230,H1_10_2022_11_30,20221216_HM2HNDRX2
231,A2_10_2022_12_01,20221216_HM2HNDRX2
232,A2_10_2022_12_08,20221223_HMLV2DRX2
233,A2_10_2022_12_15,20230105_HMLN2DRX2
234,A2_10_2022_12_22,20230105_HMLN2DRX2
235,B2_10_2022_12_02,20221216_HM2HNDRX2
236,B2_10_2022_12_09,20221223_HMLV2DRX2
237,B2_10_2022_12_16,20230105_HMLN2DRX2
238,B2_10_2022_12_23,20230105_HMLN2DRX2
239,C2_10_2022_12_03,20221216_HM2HNDRX2
240,C2_10_2022_12_10,20221223_HMLV2DRX2
241,C2_10_2022_12_17,20230105_HMLN2DRX2
242,C2_10_2022_12_24,20230105_HMLN2DRX2
243,D2_10_2022_12_04,20221216_HM2HNDRX2
244,D2_10_2022_12_11,20221223_HMLV2DRX2
245,D2_10_2022_12_18,20230105_HMLN2DRX2
246,D2_10_2022_12_25,20230105_HMLN2DRX2
247,E2_10_2022_12_05,20221216_HM2HNDRX2
248,E2_10_2022_12_12,20221223_HMLV2DRX2
249,E2_10_2022_12_19,20230105_HMLN2DRX2
250,E2_10_2022_12_26,20230105_HMLN2DRX2
251,F2_10_2022_12_06,20221216_HM2HNDRX2
252,F2_10_2022_12_13,20221223_HMLV2DRX2
253,F2_10_2022_12_20,20230105_HMLN2DRX2
254,F2_10_2022_12_27,20230105_HMLN2DRX2
255,H1_10_2022_12_07,20221223_HMLV2DRX2
256,H1_10_2022_12_14,20230105_HMLN2DRX2
257,H1_10_2022_12_21,20230105_HMLN2DRX2
Original file line number Diff line number Diff line change
Expand Up @@ -14,14 +14,14 @@ default-resources:
# - qos=<name-of-quality-of-service>
- mem_mb=2000
- runtime=15
restart-times: 3
restart-times: 1
max-jobs-per-second: 10
max-status-checks-per-second: 1
local-cores: 1
latency-wait: 60
jobs: 500
keep-going: True
rerun-incomplete: True
rerun-incomplete: False
printshellcmds: True
scheduler: greedy
use-conda: True
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