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@ARTICLE{Raspet2024, | ||
title = "Facilitating bootstrapped and rarefaction-based microbiome | ||
diversity analysis with {q2}-boots", | ||
author = "Raspet, Isaiah and Gehret, Elizabeth and Herman, Chloe and | ||
Meilander, Jeff and Manley, Andrew and Simard, Anthony and | ||
Bolyen, Evan and Caporaso, J Gregory", | ||
journal = "arXiv [q-bio.QM]", | ||
abstract = "Background: We present q2-boots, a QIIME 2 plugin that | ||
facilitates bootstrapped and rarefaction-based microbiome | ||
diversity analysis. This plugin provides eight new actions | ||
that allow users to apply any of thirty different alpha | ||
diversity metrics and twenty-two beta diversity metrics to | ||
bootstrapped or rarefied feature tables, using a single QIIME | ||
2 Pipeline command, or more granular QIIME 2 Action commands. | ||
Results: Given a feature table, an even sampling depth, and | ||
the number of iterations to perform (n), the command qiime | ||
boots core-metrics will resample the feature table n times | ||
and compute alpha and beta diversity metrics on each | ||
resampled table. The results will be integrated in summary | ||
data artifacts that are identical in structure and type to | ||
results that would be generated by applying diversity metrics | ||
to a single table. This enables all of the same downstream | ||
analytic tools to be applied to these tables, and ensures | ||
that all collected data is considered when computing | ||
microbiome diversity metrics. Conclusions: A challenge of | ||
this work was deciding how to integrate distance matrices | ||
that were computed on n resampled feature tables, as a simple | ||
average of pairwise distances (median or mean) does not | ||
account for the structure of distance matrices. q2-boots | ||
provides three options, and we show here that the results of | ||
these approaches are highly correlated. q2-boots is free and | ||
open source. Source code, installation instructions, and a | ||
tutorial can be found at | ||
https://github.com/caporaso-lab/q2-boots.", | ||
month = aug, | ||
year = 2024, | ||
archivePrefix = "arXiv", | ||
primaryClass = "q-bio.QM", | ||
doi = "https://doi.org/10.48550/arXiv.2408.05420" | ||
} |
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