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Fix: #244 Nanopore readset file reference added
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shaloo committed Jan 14, 2024
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30 changes: 30 additions & 0 deletions docs/source/user_guide/pipelines/example_runs/nanopore-readset.inc
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.. note:: Nanopore Readset Format

Use the following **readset** file format for Nanopore, Nanopore CoV-Seq pipelines:

* Sample: must contain letters A-Z, numbers 0-9, hyphens (-) or underscores (_) only; mandatory;
* Readset: a unique readset name with the same allowed characters as above; mandatory;
* Run: a unique ONT run name, usually has a structure similar to PAE0000_a1b2c3d;
* Flowcell: code of the type of flowcell used, for example: the code for PromethION Flow Cell (R9.4) is FLO-PRO002;
* Library: code of the type of library preparation kit used, for example: the code for the Ligation Sequencing Kit is SQK-LSK109;
* Summary: path to the sequencing_summary.txt file outputted by the ONT basecaller; mandatory;
* FASTQ: mandatory, path to the fastq_pass directory, that is usually created by the basecaller
* FAST5: path to the directory containing the raw fast5 files, before basecalling

Example:

+--------+----------+------------------+-----------------+-------------+----------------------------------------+-----------------------------+-----------------------------+
|Sample | Readset | Run | Flowcell | Library | Summary | FASTQ | FAST5 |
+--------+----------+------------------+-----------------+-------------+----------------------------------------+-----------------------------+-----------------------------+
|sampleA | readset1 | PAE00001_abcd123 | FLO-PRO002 | SQK-LSK109 |path/to/readset1_sequencing_summary.txt | path/to/readset1/fastq_pass | path/to/readset1/fast5_pass |
+--------+----------+------------------+-----------------+-------------+----------------------------------------+-----------------------------+-----------------------------+
|sampleA |readset2 | PAE00002_abcd456 | FLO-PRO002 | SQK-LSK109 |path/to/readset2_sequencing_summary.txt | path/to/readset2/fastq_pass | path/to/readset2/fast5_pass |
+--------+----------+------------------+-----------------+-------------+----------------------------------------+-----------------------------+-----------------------------+
|sampleA |readset3 | PAE00003_abcd789 | FLO-PRO002 | SQK-LSK109 |path/to/readset3_sequencing_summary.txt | path/to/readset3/fastq_pass | path/to/readset3/fast5_pass |
+--------+----------+------------------+-----------------+-------------+----------------------------------------+-----------------------------+-----------------------------+
|sampleA |readset4 | PAE00004_abcd246 | FLO-PRO002 | SQK-LSK109 |path/to/readset4_sequencing_summary.txt | path/to/readset4/fastq_pass | path/to/readset4/fast5_pass |
+--------+----------+------------------+-----------------+-------------+----------------------------------------+-----------------------------+-----------------------------+




4 changes: 3 additions & 1 deletion docs/source/user_guide/pipelines/gp_nanopore.rst
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Expand Up @@ -84,7 +84,9 @@ Use the following commands to execute Nanopore Sequencing Pipeline:

.. include:: /user_guide/pipelines/notes/scriptfile_deprecation.inc

You can download the test dataset for this pipeline :ref:`here<docs_testdatasets>`. Nanopore pipeline readset file structure and format details are available `here <https://bitbucket.org/mugqic/genpipes/src/master/README.md#markdown-header-nanopore-pipeline>`_.
.. include:: /user_guide/pipelines/example_runs/nanopore-readset.inc

You can download the test dataset for this pipeline :ref:`here<docs_testdatasets>`.

----

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4 changes: 3 additions & 1 deletion docs/source/user_guide/pipelines/gp_nanopore_covseq.rst
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Expand Up @@ -93,7 +93,9 @@ Use the following commands to execute Nanopore sequencing pipeline:

.. include:: /user_guide/pipelines/notes/artic_version.inc

You can download the test dataset for this pipeline :ref:`here<docs_testdatasets>`. Nanopore CoVSeQ readset file structure and content details are available `here <https://bitbucket.org/mugqic/genpipes/src/master/README.md#markdown-header-nanopore-nanopore-cov-seq>`_.
.. include:: /user_guide/pipelines/example_runs/nanopore-readset.inc

You can download the test dataset for this pipeline :ref:`here<docs_testdatasets>`.

----

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