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Update README.md
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bpickett authored Mar 2, 2023
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Expand Up @@ -26,7 +26,8 @@ If the enrichment was calculated with SPIA, the csv file should be used as the i

# Running Pathway2Targets
The Pathways2Targets.R script requires the graphite, biomaRt, RCurl, stringr, jsonlite, and httr libraries in R and Bioconductor. The following command should be used to run the script from the command-line:
Rscript --vanilla Pathway2Target.R <path_to_significant_pathway_file>

### Rscript --vanilla Pathway2Target.R <path_to_significant_pathway_file>

Before running this software, the pathway information from the Reactome database should be stored locally through the R graphite library. To do so, load the graphite library in R and use the following command for Reactome:
prepareSPIA(humanReactome, "Reactome", print.names=TRUE)
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