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tavareshugo authored May 10, 2024
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# Introduction to single-cell RNA-seq data analysis

### 16, 17, 20 Feb || 09:30 - 17:30
### Bioinformatics Training Facility, Craik-Marshall Building, Downing Site, University of Cambridge
### 16th, 17th and 20th May 2024 || 09:30 - 17:30
#### In-person at the Craik Marshall training room ([map](https://maps.app.goo.gl/wJLCDC7XU67vUrEF7))

![](UnivCambridge_ScRnaSeqIntro_Base/Images/uniOfCamCrukLogos.png)

## Instructors

* Abigail Edwards - Bioinformatics Core, Cancer Research UK Cambridge Institute
* **Invited speaker:** Katarzyna Kania - CoSyne Therapeutics

* Adam Reid - Bioinformatics Core, Gurdon Institute
* Ash Sawle - Bioinformatics Core, Cancer Research UK Cambridge Institute
* Betty Wang - Department Clinical Neurosciences
* Chandra Chilamakuri - Bioinformatics Core, Cancer Research UK Cambridge Institute
* Hugo Tavares - Bioinformatics Training Facility, Cambridge
* Jiawei Wang - EBI
* Jiayin Hong - Department of Biochemistry
* Jon Price - Biochemistry Department, University of Cambridge
* Katarzyna Kania - Genomics Core, Cancer Research UK Cambridge Institute


## Outline
Expand Down Expand Up @@ -45,7 +47,7 @@ of gene expression data, such as TSNE, UMAP and violin plots.
relating to pediatric leukemia, with four sample types, including:
* pediatric Bone Marrow Mononuclear Cells (PBMMCs)
* three tumour types: ETV6-RUNX1, HHD, PRE-T
* The data used in the course can be [downloaded from Dropbox](https://www.dropbox.com/sh/qwxgat50tsg8m3r/AAAzQd5WXuEBGOFl3m4f5B1La?dl=1) (the file is 4.2GB compressed and XXGB when uncompressed, so make sure you have enough space on your computer). Please note that:
* The data used in the course can be [downloaded from Dropbox](https://www.dropbox.com/scl/fo/9uu4iuapr60jfu2bvggdz/h?rlkey=64vrtse3lm8eb3zu9ovlxdp1a&st=t9e1bm9x&dl=0). Please note that:
* these data have been processed for teaching purposes and are therefore not suitable for research use;
* all the data is provided on our training machines, you don't need to download it to attend the course.

Expand All @@ -55,37 +57,37 @@ of gene expression data, such as TSNE, UMAP and violin plots.

### Day 1

Trainers: Abbi, Adam, Jiayin, Jon
Trainers: Adam, Chandra, Jiawei

* 09:30 - 09:40 **Welcome** - Abbi
* 09:30 - 09:40 **Welcome** - Hugo
* 09:40 - 10:25 **Introduction to Single Cell Technologies** - Katarzyna Kania
+ [Slides](UnivCambridge_ScRnaSeqIntro_Base/Slides/01_Introduction.pdf)
* 10:25 - 10:30 - **Break**
* 10:30 - 10:40 **Preamble**: data set and workflow - Jon
* 10:30 - 10:40 **Preamble**: data set and workflow - Chandra
+ [Slides](UnivCambridge_ScRnaSeqIntro_Base/Slides/02_PreambleSlides.html)
* 10:40 - 12:30 Library structure, **cellranger** for alignment and cell calling - Jon
* 10:40 - 12:30 Library structure, **cellranger** for alignment and cell calling - Chandra
+ [Slides](UnivCambridge_ScRnaSeqIntro_Base/Slides/03_CellRangerSlides.html) \([pdf](UnivCambridge_ScRnaSeqIntro_Base/Slides/03_CellRangerSlides.pdf)\)
+ [Demonstration](UnivCambridge_ScRnaSeqIntro_Base/Markdowns/03_CellRanger.html)
* 12:30 - 13:30 **Lunch break**
* 13:30 - 17:00 **QC and exploratory analysis** - Adam
+ [Slides](UnivCambridge_ScRnaSeqIntro_Base/Slides/04_QualityControlSlides.html) \([pdf](UnivCambridge_ScRnaSeqIntro_Base/Slides/04_QualityControlSlides.pdf)\)
+ [Demonstration](UnivCambridge_ScRnaSeqIntro_Base/Markdowns/04_Preprocessing_And_QC.html)
+ [Demo live script](live_scripts/04_demonstration_live_script.R)
<!-- + [Demo live script](live_scripts/04_demonstration_live_script.R) -->
+ [Practical](UnivCambridge_ScRnaSeqIntro_Base/Markdowns/04_Preprocessing_And_QC.Exercise.html)
+ [Exercise live script](live_scripts/04_exercise_live_script.R)
<!-- + [Exercise live script](live_scripts/04_exercise_live_script.R) -->

### Day 2

Trainers: Adam, Jiawei, Jiayin, Jon
Trainers: Adam, Chandra, Hugo

* 09:30 - 09:40 **Recap** - Jon
* 09:30 - 09:40 **Recap** - Chandra
+ [Slides](UnivCambridge_ScRnaSeqIntro_Base/Slides/00_Day1_Recap.html)
* 09:40 - 12:30 **Normalisation** - Jon
* 09:40 - 12:30 **Normalisation** - Chandra
+ [Slides](UnivCambridge_ScRnaSeqIntro_Base/Slides/05_NormalisationSlides.html) \([pdf](UnivCambridge_ScRnaSeqIntro_Base/Slides/05_NormalisationSlides.pdf)\)
+ [Demonstration](UnivCambridge_ScRnaSeqIntro_Base/Markdowns/05_Normalisation.html)
+ [Practical](UnivCambridge_ScRnaSeqIntro_Base/Markdowns/05_Normalisation_exercises.html)
* 12:30 - 13:30 **lunch break**
* 13:30 - 15:25 **Feature selection and dimensionality reduction** - Jiayin / Hugo
* 13:30 - 15:25 **Feature selection and dimensionality reduction** - Hugo
+ [Slides](UnivCambridge_ScRnaSeqIntro_Base/Slides/06_FeatureSelectionAndDimensionalityReduction_slides.html) \([pdf](UnivCambridge_ScRnaSeqIntro_Base/Slides/06_FeatureSelectionAndDimensionalityReduction_slides.pdf)\)
+ [Demonstration](UnivCambridge_ScRnaSeqIntro_Base/Markdowns/06_FeatureSelectionAndDimensionalityReduction.html)
* 15:25 - 15:35 10 min **break**
Expand All @@ -95,18 +97,18 @@ Trainers: Adam, Jiawei, Jiayin, Jon

### Day 3

Trainers: Abbi, Adam, Jiawei, Jiayin
Trainers: Ash, Betty, Hugo, Jiawei

* 09:30 - 09:40 Recap - Adam
* 09:40 - 11:05 **Clustering** - Adam
* 09:30 - 09:40 Recap - Hugo
* 09:40 - 11:05 **Clustering** - Jiawei / Hugo
+ [Slides](UnivCambridge_ScRnaSeqIntro_Base/Slides/08_ClusteringSlides.html)
+ [Demonstration](UnivCambridge_ScRnaSeqIntro_Base/Markdowns/08_Clustering.html)
* 11:05 - 11:15 10 min **break**
* 11:15 - 12:30 **Identification of cluster marker genes** - Abbi
* 11:15 - 12:30 **Identification of cluster marker genes** - Jiawei / Hugo
+ [Slides](UnivCambridge_ScRnaSeqIntro_Base/Slides/09_ClusterMarkerGenes.html)
+ [Demonstration](UnivCambridge_ScRnaSeqIntro_Base/Markdowns/09_Cluster_Marker_Genes.html)
* 12:30 - 13:30 **lunch break**
* 13:30 - 17.30 **Differential Expression and Abundance Analysis** - Abbi
* 13:30 - 17.30 **Differential Expression and Abundance Analysis** - Ash
+ [Slides](UnivCambridge_ScRnaSeqIntro_Base/Slides/10_DifferentialExpressionAndAbundance.pdf)
+ [Demonstration - Differential Expression](UnivCambridge_ScRnaSeqIntro_Base/Markdowns/10_Differential_Expression.html)
+ [Demonstration - Differential Abundance](UnivCambridge_ScRnaSeqIntro_Base/Markdowns/11_Differential_Abundance.html)
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