Error in opening folder for segmentation

[NidhiChopdekar]

Description:

Hello,
After I try to launch the GUI and try to open my positions folder, it asks me for the channel name. I am not exactly sure what I must provide but my dataset has a channel w-50 in its name, so i just put that (have tried other channels as well) but i end up getting an error saying

Error in function openFolder. Traceback (most recent call last):
File "C:\Users\Nidhi\anaconda3\envs\acdc\lib\site-packages\cellacdc_init.py", line 403, in inner_function
result = func(self, *args, **kwargs)
File "C:\Users\Nidhi\anaconda3\envs\acdc\lib\site-packages\cellacdc\gui.py", line 22765, in _openFolder
self.gui_createOverlayColors()
File "C:\Users\Nidhi\anaconda3\envs\acdc\lib\site-packages\cellacdc\gui.py", line 4192, in gui_createOverlayColors
self.overlayColors[ch] = self.overlayRGBs[c]
IndexError: list index out of range

Please help me out

Thanks
Nidhi

.2024-04-21_03-10-42_acdc_gui_0a706c29-7d3c-42d0-8581-82da1de9d6a1_stdout.log

Way to reproduce:

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(Optional) Link to data to reproduce error:

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Terminal output:

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Log file:

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Version information:

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[ElpadoCan]

Hi Nidhi,

I'm sorry to hear you are having this issue, but we will fix it.

It sounds like Cell-ACDC wants to load more than 12 additional channels, but I'm guessing you do not have that many, right?

Could you please send me a screenshot of the files you have in the Experiment_folder/Position_1 folder? Thanks!

Best,
Francesco

[ElpadoCan]

I just realised that the tif file you are loading ends with t050 suggesting that you have one tif file per time point? That will not work in acdc, you will need to concatenate the tiff files into a single file with all the time points. You will need one tif file per channel.

You can convert the files into acdc-compatible structure with module 0. Create data structure from microscopy/image files(s).... You will then choose Re-structure image files and follow the wizard. Let me know if it works thanks

Best
Francesco

[github-actions]

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