This self-guided workflow tutorial will cover analysis of Illumina MiSeq data from initial reads to amplicon sequence variants (ASVs), taxonomy, and visualization. The workflow uses data generated by Maine-eDNA researchers for 18S using the Comeau et al., 2011 primers E572F/E1009R for the V4 region. A containerized environment was created in CyVerse for software setup, which uses FastQC, Cutadapt, and R packages for analysis and visualization {Dada2, Phyloseq, GGplot2}.
For more information on using CyVerse, see the help documentation here.
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