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Reference annotation database construction get's stuck #23

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wrengs opened this issue Aug 13, 2024 · 0 comments
Open

Reference annotation database construction get's stuck #23

wrengs opened this issue Aug 13, 2024 · 0 comments

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@wrengs
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wrengs commented Aug 13, 2024

Dear Kuanhao-Chao,

I installed LiftOn according to the documentation, checked installation by successfully invoking the help page and am now trying LiftOn on two tomato genomes to annotate a new reference.

The used GFF file.
https://solgenomics.net/ftp/genomes/Solanum_lycopersicum/Heinz1706/annotation/ITAG4.0_release/ITAG4.0_gene_models.gff
and command:
lifton -g ITAG4.0_gene_models.gff -mm2_options="-a --end-bonus 5 --eqx -N 50 -p 0.5 -t 34" -t 34 -o SL5_ITAG4.gff3 -copies -sc 0.95 SL5.0.fasta SL4.0.fasta

SL5.0 = https://solgenomics.net/ftp/genomes/TGG/genome/SL5.0.fasta.gz
SL4.0 = https://solgenomics.net/ftp/genomes/Solanum_lycopersicum/Heinz1706/assembly/build_4.00/S_lycopersicum_chromosomes.4.00.fa

0.13-r248
>> Reading target genome ...
>> Reading reference genome ...

>> Creating reference annotation database :  ITAG4.0_gene_models.gff

I left the tool to run over the weekend, however it seems to get stuck at reference annotation database creation, with leading process entering D state and all others S.

Any suggestions would be most welcome.

Cheers,
Willem

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