IBEXImagingCommunity · zivy · Oct 31, 2023 · Oct 31, 2023
diff --git a/data/reagent_resources.csv b/data/reagent_resources.csv
@@ -620,11 +620,11 @@ Q9UKI9,Primary Antibody,POU2F3,Human,Rabbit,IgG,Polyclonal,Novus Biologicals,NBP
 Q12912,Primary Antibody,LRMP,Human,Rabbit,IgG,EPR17852,Abcam,ab202418,Unconjugated,NA,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
 Q76IQ4,Primary Antibody,Ghrelin,Human,Mouse,IgG2b,891240,R&D Systems,MAB8149,Unconjugated,NA,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Jejunum,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
 P13569,Primary Antibody,CFTR,Human,Mouse,IgM,CF3,Thermo Fisher Scientific,MA1-935,Unconjugated,AB_2081230,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Pancreas,NA,0.3% Triton-X-100,NA,NA,No,0000-0002-2950-2683,NA,0000-0002-2950-2683
-P13569,Primary Antibody,CFTR,Human,Mouse,IgM,CF3,Thermo Fisher Scientific,MA1-935,Unconjugated,AB_2081230,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond,NA,Yes,0000-0002-2950-2683,NA,0000-0002-2950-2683
+P13569,Primary Antibody,CFTR,Human,Mouse,IgM,CF3,Thermo Fisher Scientific,MA1-935,Unconjugated,AB_2081230,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond,NA,Yes,0000-0002-2950-2683,NA,0000-0002-2950-2683
 P13569,Primary Antibody,CFTR,Human,Mouse,IgG1,M3A7,Thermo Fisher Scientific,MA5-11768,Unconjugated,AB_10983660,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Pancreas,NA,0.3% Triton-X-100,NA,NA,No,0000-0002-2950-2683,NA,0000-0002-2950-2683
 P01833,Primary Antibody,PIGR,Human,Rabbit,IgG,Polyclonal,Abcam,ab96196,Unconjugated,AB_10677612,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Pancreas,NA,0.3% Triton-X-100,NA,NA,No,0000-0002-2950-2683,NA,0000-0002-2950-2683
 P01833,Primary Antibody,PIGR,Human,Rat,IgG1,7C1,Abcam,ab170321,Unconjugated,NA,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Jejunum,NA,0.3% Triton-X-100,NA,NA,No,0000-0002-2950-2683,NA,0000-0002-2950-2683
-P01833,Primary Antibody,PIGR,Human,Rat,IgG1,7C1,Abcam,ab170321,Unconjugated,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond,NA,Yes,0000-0002-2950-2683,NA,0000-0002-2950-2683
+P01833,Primary Antibody,PIGR,Human,Rat,IgG1,7C1,Abcam,ab170321,Unconjugated,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond,NA,Yes,0000-0002-2950-2683,NA,0000-0002-2950-2683
 P01833,Primary Antibody,PIGR,Human,Rabbit,IgG,Polyclonal,Thermo Fisher Scientific,PA5-35340,Unconjugated,AB_2552650,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,NA,No,0000-0002-2950-2683,NA,0000-0002-2950-2683
 P07476,Primary Antibody,Involucrin,Human,Mouse,IgG1,SY5,Santa Cruz Biotechnology,sc-21748 AF488,AF488,AB_2128119,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Skin,NA,0.3% Triton-X-100,NA,NA,No,0000-0002-2950-2683,NA,0000-0002-2950-2683
 P07476,Primary Antibody,Involucrin,Human,Rabbit,IgG,Polyclonal,Abcam,ab53112,Unconjugated,AB_880813,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0002-2950-2683,NA,0000-0002-2950-2683
@@ -966,7 +966,7 @@ P62736,Primary Antibody,Alpha smooth muscle actin,Human,Mouse,IgG2a,1A4,Thermo F
 P62736,Primary Antibody,Alpha smooth muscle actin,Human,Mouse,IgG2a,1A4,Thermo Fisher Scientific,50-9760-82,eF660,AB_2574362,Stock,IBEX2D Manual,FFPE,Lung,Infected,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-3697-5726,NA,0000-0003-3697-5726
 NA,Primary Antibody,Cytokeratin (Pan),Human,Mouse,IgG1,AE1/AE3,Thermo Fisher Scientific,50-9003-82,eF660,AB_2574301,Stock,IBEX2D Manual,FFPE,Lung,Infected,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-3697-5726,NA,0000-0003-3697-5726
 P08670,Primary Antibody,Vimentin,Human,Mouse,IgG2a,O91D3,BioLegend,677804,AF594,AB_2566179,Stock,IBEX2D Manual,FFPE,Lung,Infected,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 for more than 120 minutes,Yes,0000-0003-3697-5726,NA,0000-0003-3697-5726
-P20023,Primary Antibody,CD21,Human,Rabbit,IgG,SP186,Abcam,ab306325,PE,NA,Stock,Cell DIVE-IBEX,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond,1 mg/ml LiBH4 15 minutes,No,0000-0003-4379-8967,NA,0000-0003-4379-8967
+P20023,Primary Antibody,CD21,Human,Rabbit,IgG,SP186,Abcam,ab306325,PE,NA,Stock,Cell DIVE-IBEX,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond,1 mg/ml LiBH4 15 minutes,No,0000-0003-4379-8967,NA,0000-0003-4379-8967
 P56199,Primary Antibody,CD49a,Human,Mouse,IgG1,CL7207,Abcam,ab243032,Unconjugated,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
 P56199,Primary Antibody,CD49a,Human,Mouse,IgG1,CL7207,Abcam,ab243032,Unconjugated,NA,Stock,Cell DIVE-IBEX,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
 NA,Secondary Antibody,Fluorescein/Oregon Green,Human,Rabbit,IgG,Polyclonal,Thermo Fisher Scientific,A-11090,AF488,AB_221562,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967

diff --git a/docs/supporting_material/CD21_PE/0000-0003-4379-8967.md b/docs/supporting_material/CD21_PE/0000-0003-4379-8967.md
@@ -8,7 +8,7 @@ layout: default
 |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|:-----------------|:------------|:-----------|:---------------|:---------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------------------------------------------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:--------------------|:------------|
 | P20023                     | Primary Antibody | CD21                              | Human            | Mouse           | IgG1      | Bu32        | BioLegend | 354903           | PE          | AB_2561406 | Stock          | IBEX2D Manual  | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node      | NA             | 0.3% Triton-X-100 | NA                                                                                         | 1 mg/ml LiBH4 15 minutes      | Yes         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications), [1](#publications)] | NA         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) |
 | P20023                     | Primary Antibody | CD21                              | Human            | Rabbit          | IgG       | SP186       | Abcam     | ab306325         | PE          | NA         | Stock          | Cell DIVE-IBEX | FFPE                              | Lymph Node          | Follicular Lymphoma         | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes      | Yes         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967)                                          | NA         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) |
-| P20023                     | Primary Antibody | CD21                              | Human            | Rabbit          | IgG       | SP186       | Abcam     | ab306325         | PE          | NA         | Stock          | Cell DIVE-IBEX | FFPE                              | Lymph Node       | Follicular Lymphoma       | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond                                      | 1 mg/ml LiBH4 15 minutes      | No          | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967)                                          | NA         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) |
+| P20023                     | Primary Antibody | CD21                              | Human            | Rabbit          | IgG       | SP186       | Abcam     | ab306325         | PE          | NA         | Stock          | Cell DIVE-IBEX | FFPE                              | Lymph Node       | Follicular Lymphoma       | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond                                      | 1 mg/ml LiBH4 15 minutes      | No          | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967)                                          | NA         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) |
 
 # Publications
 

diff --git a/docs/supporting_material/CFTR_Unconjugated/0000-0002-2950-2683.md b/docs/supporting_material/CFTR_Unconjugated/0000-0002-2950-2683.md
@@ -7,7 +7,7 @@ layout: default
 | UniProt Accession Number   | Reagent Type     | Target Name / Protein Biomarker   | Target Species   | Host Organism   | Isotype   | Clonality   | Vendor                   | Catalog Number   | Conjugate    | RRID        | Availability   | Method                 | Tissue Preservation               | Target Tissue   | Tissue State   | Detergent         | Antigen Retrieval Conditions                          | Dye Inactivation Conditions   | Recommend   | Agree               | Disagree   | Contributor         | Notes       |
 |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:-------------|:------------|:---------------|:-----------------------|:----------------------------------|:----------------|:---------------|:------------------|:------------------------------------------------------|:------------------------------|:------------|:--------------------|:-----------|:--------------------|:------------|
 | P13569                     | Primary Antibody | CFTR                              | Human            | Mouse           | IgM       | CF3         | Thermo Fisher Scientific | MA1-935          | Unconjugated | AB_2081230  | Stock          | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas        | NA             | 0.3% Triton-X-100 | NA                                                    | NA                            | No          | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA         | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [1](#notes) |
-| P13569                     | Primary Antibody | CFTR                              | Human            | Mouse           | IgM       | CF3         | Thermo Fisher Scientific | MA1-935          | Unconjugated | AB_2081230  | Stock          | Multiplexed 2D Imaging | FFPE                              | Tonsil          | NA             | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond | NA                            | Yes         | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA         | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [2](#notes) |
+| P13569                     | Primary Antibody | CFTR                              | Human            | Mouse           | IgM       | CF3         | Thermo Fisher Scientific | MA1-935          | Unconjugated | AB_2081230  | Stock          | Multiplexed 2D Imaging | FFPE                              | Tonsil          | NA             | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | NA                            | Yes         | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA         | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [2](#notes) |
 | P13569                     | Primary Antibody | CFTR                              | Human            | Mouse           | IgG1      | M3A7        | Thermo Fisher Scientific | MA5-11768        | Unconjugated | AB_10983660 | Stock          | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas        | NA             | 0.3% Triton-X-100 | NA                                                    | NA                            | No          | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA         | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [3](#notes) |
 
 # Publications

diff --git a/docs/supporting_material/PIGR_Unconjugated/0000-0002-2950-2683.md b/docs/supporting_material/PIGR_Unconjugated/0000-0002-2950-2683.md
@@ -8,7 +8,7 @@ layout: default
 |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:-------------|:------------|:---------------|:-----------------------|:----------------------------------|:----------------|:---------------|:------------------|:------------------------------------------------------|:------------------------------|:------------|:--------------------|:-----------|:--------------------|:------------|
 | P01833                     | Primary Antibody | PIGR                              | Human            | Rabbit          | IgG       | Polyclonal  | Abcam                    | ab96196          | Unconjugated | AB_10677612 | Stock          | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas        | NA             | 0.3% Triton-X-100 | NA                                                    | NA                            | No          | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA         | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [1](#notes) |
 | P01833                     | Primary Antibody | PIGR                              | Human            | Rat             | IgG1      | 7C1         | Abcam                    | ab170321         | Unconjugated | NA          | Stock          | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum         | NA             | 0.3% Triton-X-100 | NA                                                    | NA                            | No          | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA         | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [2](#notes) |
-| P01833                     | Primary Antibody | PIGR                              | Human            | Rat             | IgG1      | 7C1         | Abcam                    | ab170321         | Unconjugated | NA          | Stock          | Multiplexed 2D Imaging | FFPE                              | Tonsil          | NA             | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond | NA                            | Yes         | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA         | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [3](#notes) |
+| P01833                     | Primary Antibody | PIGR                              | Human            | Rat             | IgG1      | 7C1         | Abcam                    | ab170321         | Unconjugated | NA          | Stock          | Multiplexed 2D Imaging | FFPE                              | Tonsil          | NA             | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | NA                            | Yes         | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA         | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [3](#notes) |
 | P01833                     | Primary Antibody | PIGR                              | Human            | Rabbit          | IgG       | Polyclonal  | Thermo Fisher Scientific | PA5-35340        | Unconjugated | AB_2552650  | Stock          | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus          | NA             | 0.3% Triton-X-100 | NA                                                    | NA                            | No          | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA         | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [4](#notes) |
 
 # Publications