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Adding reagent validations from Koenig lab, McMaster University.
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docs/supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0002-1461-0999.md
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# Configurations | ||
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| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | | ||
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| | ||
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967), [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | | ||
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# Publications | ||
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# Additional Notes | ||
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<a name="notes"></a> | ||
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| Mouse small intestine: alpha smooth muscle actin (grey, catalogue number 53-9760-82) | | ||
|:-------:| | ||
| ![](aSMA_AF488_Grey_Mouse_SI.jpg) | |
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...upporting_material/Alpha_smooth_muscle_actin_AF488/aSMA_AF488_Grey_Mouse_SI.jpg
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docs/supporting_material/B220_AF647/0000-0002-1461-0999.md
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# Configurations | ||
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| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | | ||
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| | ||
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Biolegend | 103226 | AF647 | AB_389330 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | | | ||
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# Publications | ||
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# Additional Notes | ||
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<a name="notes"></a> | ||
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| Mouse lymph node: B220 (grey, catalog number 103226) | | ||
|:-------:| | ||
| ![](B220_AF647_Grey_Mouse_LN.jpg) | |
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layout: default | ||
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# Configurations | ||
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| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | | ||
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| | ||
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BD Biosciences | 101217 | PE | AB_396680 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) | NA | [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) | | | ||
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# Publications | ||
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# Additional Notes | ||
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<a name="notes"></a> | ||
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| Mouse lymph node: CD11b (grey, catalog number 101217) | | ||
|:-------:| | ||
| ![](CD11b_PE_Grey_Mouse_LN.jpg) | |
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docs/supporting_material/CD11c_AF488/0000-0002-1461-0999.md
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layout: default | ||
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# Configurations | ||
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| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | | ||
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:-----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:------------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| | ||
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | Thermo Fisher Scientific | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | | | ||
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# Publications | ||
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# Additional Notes | ||
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<a name="notes"></a> | ||
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| Mouse lymph node: CD11c (grey, catalog number MCD11c20) | | ||
|:-------:| | ||
| ![](CD11c_AF488_Grey_Mouse_LN.jpg) | |
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docs/supporting_material/CD200_BV421/0000-0002-1461-0999.md
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layout: default | ||
--- | ||
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# Configurations | ||
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| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | | ||
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| | ||
| Q9ES57 | Primary Antibody | CD200 | Mouse | Rat | IgG2a | OX-90 | BD Biosciences | 565547 | BV421 | AB_2739289 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | | ||
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# Publications | ||
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# Additional Notes | ||
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<a name="notes"></a> | ||
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| Mouse lymph node: CD11c (grey, catalog number 565547) | | ||
|:-------:| | ||
| ![](CD200_BV421_Grey_Mouse_LN.jpg) | |
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