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Merge pull request #149 from zivy/removeDuplicates
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Removing duplicate reagent validation configurations.
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@zivy
zivy authored May 15, 2024
2 parents c7b43d1 + a2b5cd6 commit e72750e
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Showing 8 changed files with 6 additions and 45 deletions.
9 changes: 0 additions & 9 deletions data/reagent_resources.csv
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Original file line number Diff line number Diff line change
Expand Up @@ -779,18 +779,12 @@ P20152,Primary Antibody,Vimentin,Mouse,Rat,IgG2a,W16220A,BioLegend,699308,AF647,
P56528,Primary Antibody,CD38,Mouse,Rat,IgG2a,90,BioLegend,102732,BV421,AB_2734153,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0002-1461-0999,NA,0000-0002-1461-0999
P19437,Primary Antibody,CD20,Mouse,Rat,IgG2b,SA275A11,BioLegend,150432,AF488,AB_2890699,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,No,0000-0002-1461-0999,NA,0000-0002-1461-0999
Q61503,Primary Antibody,CD73,Mouse,Rat,IgG1,TY/11.8,BioLegend,127230,AF700,AB_2800625,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,No,0000-0003-2634-6838,NA,0000-0002-1461-0999
P01732,Primary Antibody,CD8,Human,Mouse,IgG1,C8/144B,BioLegend,372906,AF647,AB_2650712,Stock,IBEX2D Manual,FFPE,Heart,NA,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4184-3867,NA,0000-0003-4184-3867
P01732,Primary Antibody,CD8,Human,Mouse,IgG1,C8/144B,BioLegend,372906,AF647,AB_2650712,Stock,IBEX2D Manual,FFPE,Skin,Infected,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4184-3867,NA,0000-0003-4184-3867
P01732,Primary Antibody,CD8,Human,Mouse,IgG1,C8/144B,BioLegend,372906,AF647,AB_2650712,Stock,IBEX2D Manual,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4184-3867,NA,0000-0003-4184-3867
P01732,Primary Antibody,CD8,Human,Mouse,IgG1,C8/144B,BioLegend,372906,AF647,AB_2650712,Stock,IBEX2D Manual,FFPE,Cervix,Cervical Cancer,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4184-3867,NA,0000-0003-4184-3867
P07766,Primary Antibody,CD3,Human,Rabbit,IgG,EP4426,Abcam,ab208514,AF555,AB_443425,Stock,IBEX2D Manual,FFPE,Cervix,Cervical Cancer,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4184-3867,NA,0000-0003-4184-3867
P07766,Primary Antibody,CD3,Human,Rabbit,IgG,EP4426,Abcam,ab208514,AF555,AB_443425,Stock,IBEX2D Manual,FFPE,Skin,NA,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4184-3867,NA,0000-0003-4184-3867
P01732,Primary Antibody,CD8,Human,Mouse,IgG1,C8/144B,BioLegend,372906,AF647,AB_2650712,Stock,IBEX2D Manual,FFPE,Heart,NA,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0002-7586-9996,NA,0000-0002-7586-9996
P01732,Primary Antibody,CD8,Human,Mouse,IgG1,C8/144B,BioLegend,372906,AF647,AB_2650712,Stock,IBEX2D Manual,FFPE,Skin,Infected,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0002-7586-9996,NA,0000-0002-7586-9996
P01732,Primary Antibody,CD8,Human,Mouse,IgG1,C8/144B,BioLegend,372906,AF647,AB_2650712,Stock,IBEX2D Manual,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0002-7586-9996,NA,0000-0002-7586-9996
P01732,Primary Antibody,CD8,Human,Mouse,IgG1,C8/144B,BioLegend,372906,AF647,AB_2650712,Stock,IBEX2D Manual,FFPE,Cervix,Cervical Cancer,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0002-7586-9996,NA,0000-0002-7586-9996
P07766,Primary Antibody,CD3,Human,Rabbit,IgG,EP4426,Abcam,ab208514,AF555,AB_443425,Stock,IBEX2D Manual,FFPE,Cervix,Cervical Cancer,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0002-7586-9996,NA,0000-0002-7586-9996
P07766,Primary Antibody,CD3,Human,Rabbit,IgG,EP4426,Abcam,ab208514,AF555,AB_443425,Stock,IBEX2D Manual,FFPE,Skin,NA,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0002-7586-9996,NA,0000-0002-7586-9996
Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,BioLegend,117371,Spark Red 718,AB_2924457,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967
P22646,Primary Antibody,CD3,Mouse,Rat,IgG2b,17A2,BioLegend,100281,Spark Red 718,AB_2924440,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
P22646,Primary Antibody,CD3,Mouse,Rat,IgG2b,17A2,BioLegend,100281,Spark Red 718,AB_2924440,Stock,Ce3D,4% PFA Fixed Agarose,Lymph Node,NA,1:10 BD PermWash,NA,NA,Yes,0000-0002-5187-810X,NA,0000-0002-5187-810X
Expand Down Expand Up @@ -961,7 +955,6 @@ NA,Secondary Antibody,Sheep IgG (H+L),Human,Donkey,IgG,Polyclonal,Thermo Fisher
P07766,Primary Antibody,CD3,Human,Mouse,IgG1,UCHT1,AAT Bioquest,10032060,iF514,NA,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
NA,Avidin/Biotin Blocking Kit,NA,Human,NA,NA,NA,Abcam,ab64212,NA,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
NA,Avidin/Biotin Blocking Kit,NA,Human,NA,NA,NA,Abcam,ab64212,NA,NA,Stock,Cell DIVE-IBEX,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
P62736,Primary Antibody,Alpha smooth muscle actin,Human,Mouse,IgG2a,1A4,Thermo Fisher Scientific,53-9760-82,AF488,AB_2574461,Stock,IBEX2D Manual,FFPE,Lung,Infected,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-3697-5726,NA,0000-0003-3697-5726
P62736,Primary Antibody,Alpha smooth muscle actin,Human,Mouse,IgG2a,1A4,Thermo Fisher Scientific,41-9760-82,eF570,AB_2573631,Stock,IBEX2D Manual,FFPE,Lung,Infected,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-3697-5726,NA,0000-0003-3697-5726
P62736,Primary Antibody,Alpha smooth muscle actin,Human,Mouse,IgG2a,1A4,Thermo Fisher Scientific,50-9760-82,eF660,AB_2574362,Stock,IBEX2D Manual,FFPE,Lung,Infected,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-3697-5726,NA,0000-0003-3697-5726
NA,Primary Antibody,Cytokeratin (Pan),Human,Mouse,IgG1,AE1/AE3,Thermo Fisher Scientific,50-9003-82,eF660,AB_2574301,Stock,IBEX2D Manual,FFPE,Lung,Infected,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-3697-5726,NA,0000-0003-3697-5726
Expand Down Expand Up @@ -1049,7 +1042,6 @@ P01903,Primary Antibody,HLA-DR,Human,Mouse,IgG2b,LN-3,Novus Biologicals,NBP2-476
Q15116,Primary Antibody,PD-1,Human,Rabbit,IgG,EPR4877(2),Abcam,ab275126,AF555,AB_2728811,Stock,Multiplexed 2D Imaging,FFPE,Lymph node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
P16410,Primary Antibody,CTLA4,Human,Rabbit,IgG,CAL49,Abcam,ab270127,AF647,NA,Stock,Multiplexed 2D Imaging,FFPE,Lymph node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
P22646,Primary Antibody,CD3,Mouse,Rat,IgG2b,17A2,BioLegend,100281,Spark Red 718,AB_2924440,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Spleen,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-1130-1899,NA,0000-0003-1130-1899
Q9BZS1,Primary Antibody,FOXP3,Human,Mouse,IgG1,236A/E7,Thermo Fisher Scientific,41-4777-82,eF570,AB_2573609,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967
P01903,Primary Antibody,HLA-DR,Human,Mouse,IgG2a,LN-3,Novus Biologicals,NBP2-47670AF647,AF647,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
O00329,Primary Antibody,PI3 Kinase p110 delta,Human,Rabbit,IgG,E2T2N,Cell Signaling Technology,19591S,Unconjugated,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967
P01106,Primary Antibody,c-Myc,Human,Mouse,IgG1,9E10,R&D Systems,MAB3696-SP,Unconjugated,NA,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967
Expand All @@ -1059,7 +1051,6 @@ P15104,Primary Antibody,Glutamine synthetase,Human,Mouse,IgG2a,OTI1F4,Novus Biol
P15104,Primary Antibody,Glutamine synthetase,Human,Rabbit,IgG,EPR13022(B),Abcam,Ab302584,AF488,NA,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967
Q16832,Primary Antibody,DDR2,Human,Goat,IgG,Polyclonal,R&D Systems,AF2538,Unconjugated,AB_416613,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967
P49840,Primary Antibody,Phospho-GSK-3alpha,Human,Rabbit,IgG,36E9,Cell Signaling Technology,9316,Unconjugated,AB_659836,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967
Q9Y5Y7,Primary Antibody,LYVE-1,Human,Goat,IgG,Polyclonal,R&D Systems,AF2089,Unconjugated,AB_355144,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
P08575,Primary Antibody,CD45,Human,Mouse,IgG1,2B11 + PD7/26,Novus Biologicals,NBP2-34528AF700,AF700,NA,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
Q6FHL8,Primary Antibody,CTGF,Human,Rabbit,IgG,OT11F4,FabGennix,CTGF-FITC,FITC,NA,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967
E9PVX6,Primary Antibody,Ki-67,Mouse,Rat,IgG2a,SolA15,Thermo Fisher Scientific,606-5698-82,AF660,AB_2896286,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997
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18 changes: 0 additions & 18 deletions docs/supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-3697-5726.md
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1 change: 0 additions & 1 deletion docs/supporting_material/CD3_AF555/0000-0002-7586-9996.md
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Expand Up @@ -7,7 +7,6 @@ layout: default
| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------|:-----------------|:------------|:----------|:---------------|:--------------|:----------------------|:----------------|:----------------|:------------------|:--------------------------------------------------------------|:------------------------------|:------------|:--------------------|:-----------|:--------------------|:------------|
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Cervix | Cervical Cancer | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7586-9996](https://orcid.org/0000-0002-7586-9996) | NA | [0000-0002-7586-9996](https://orcid.org/0000-0002-7586-9996) | [1](#notes) |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Skin | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7586-9996](https://orcid.org/0000-0002-7586-9996) | NA | [0000-0002-7586-9996](https://orcid.org/0000-0002-7586-9996) | [1](#notes) |

# Publications

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1 change: 0 additions & 1 deletion docs/supporting_material/CD3_AF555/0000-0003-4184-3867.md
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Expand Up @@ -6,7 +6,6 @@ layout: default

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------|:-----------------|:------------|:----------|:---------------|:--------------|:----------------------|:----------------|:----------------|:------------------|:--------------------------------------------------------------|:------------------------------|:------------|:--------------------|:-----------|:--------------------|:------------|
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Cervix | Cervical Cancer | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4184-3867](https://orcid.org/0000-0003-4184-3867) | NA | [0000-0003-4184-3867](https://orcid.org/0000-0003-4184-3867) | [1](#notes) |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Skin | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4184-3867](https://orcid.org/0000-0003-4184-3867) | NA | [0000-0003-4184-3867](https://orcid.org/0000-0003-4184-3867) | [1](#notes) |

# Publications
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