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Merge pull request #102 from zivy/addingReagents
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Adding multiple reagent configurations and supporting material.
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zivy authored Feb 9, 2024
2 parents 80a3374 + 9819c9d commit 8472b37
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5 changes: 5 additions & 0 deletions .zenodo.json
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Expand Up @@ -112,6 +112,11 @@
"name": "Koenig, Joshua F. E.",
"orcid": "0000-0002-8909-5039"
},
{
"affiliation": "National Institutes of Health",
"name": "Makranz, Chen",
"orcid": "0000-0002-6944-6997"
},
{
"affiliation": "McMaster University",
"name": "Murakami Olyntho, Vitoria",
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11 changes: 11 additions & 0 deletions data/reagent_resources.csv
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Expand Up @@ -1062,3 +1062,14 @@ P49840,Primary Antibody,Phospho-GSK-3alpha,Human,Rabbit,IgG,36E9,Cell Signaling
Q9Y5Y7,Primary Antibody,LYVE-1,Human,Goat,IgG,Polyclonal,R&D Systems,AF2089,Unconjugated,AB_355144,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
P08575,Primary Antibody,CD45,Human,Mouse,IgG1,2B11 + PD7/26,Novus Biologicals,NBP2-34528AF700,AF700,NA,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967
Q6FHL8,Primary Antibody,CTGF,Human,Rabbit,IgG,OT11F4,FabGennix,CTGF-FITC,FITC,NA,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967
E9PVX6,Primary Antibody,Ki-67,Mouse,Rat,IgG2a,SolA15,Thermo Fisher Scientific,606-5698-82,AF660,AB_2896286,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997
Q08481,Primary Antibody,CD31,Mouse,Rat,IgG2a,MEC13.3,BioLegend,102515,AF647,AB_2161030,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997
P06332,Primary Antibody,CD4,Mouse,Rat,IgG2a,RM4-5,BioLegend,100533,AF647,AB_493372,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997
P06332,Primary Antibody,CD4,Mouse,Rat,IgG2a,RM4-5,BioLegend,100533,AF647,AB_493372,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Spleen,Healthy,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997
NA,Primary Antibody,mCherrry,Mouse,Chicken,IgY,polyclonal,Novus Biologicals,NBP2-25158,NA,AB_2636881,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997
Q9EQX4,Primary Antibody,Iba-1,Mouse,Rabbit,IgG,polyclonal,FUJIFILM Wako Chemicals U.S.A. Corporation,019-19741,NA,AB_839504,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997
P63158,Primary Antibody,CXCL12,Mouse,Rabbit,IgG,polyclonal,Abcam,AB25117,NA,AB_2088164,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997
P70658,Primary Antibody,CXCR4,Mouse,Rabbit,IgG,polyclonal,Novus Biologicals,NB-100 74396,NA,AB_1048484,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997
P56485,Primary Antibody,CXCR7,Mouse,Rabbit,IgG,polyclonal,Novus Biologicals,NBP2-24779G,DyLight 488,NA,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997
Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,BioLegend,117314,AF647,AB_492850,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997
P03995,Primary Antibody,GFAP,Mouse,Mouse,IgG1,GA5,Thermo Fisher Scientific,41-9892-82,eF570,AB_2573656,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997
4 changes: 3 additions & 1 deletion data/vendor_urls.csv
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Expand Up @@ -26,4 +26,6 @@ IBT BioServices,https://www.ibtbioservices.com/
Cell IDx,https://cellidx.com/
Vector Laboratories,https://vectorlabs.com/
Agilent,https://www.agilent.com/en/dako-products
FabGennix,https://fabgennix.com/
FabGennix,https://fabgennix.com/
Synaptic Systems,https://www.sysy.com/
FUJIFILM Wako Chemicals U.S.A. Corporation,https://labchem-wako.fujifilm.com
22 changes: 22 additions & 0 deletions docs/supporting_material/CD11c_AF647/0000-0002-6944-6997.md
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---
layout: default
---

# Configurations

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:-----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------|
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117314 | AF647 | AB_492850 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) |

# Publications



# Additional Notes

<a name="notes"></a>
1. Dilution 1:25.

| Mouse Spleen Hochest (Blue) CD11c (Magenta Biolegend #117314) |
|:-------:|
| ![](Mouse_Spleen_Hochest_Blue_CD11c_Magenta_Biolegend_117314.jpg) |
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22 changes: 22 additions & 0 deletions docs/supporting_material/CD31_AF647/0000-0002-6944-6997.md
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---
layout: default
---

# Configurations

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------|
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102515 | AF647 | AB_2161030 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) |

# Publications



# Additional Notes

<a name="notes"></a>
1. Dilution 1:100.

| Mouse Brain Tumor Hochest(Blue) mCherry (Green Novus #NBP2-25158) CD31(Magenta Biolegend #102515) |
|:-------:|
| ![](Mouse_Brain_Tumor_Hochest_Blue_mCherry_Green_Novus_NBP2_25158_CD31_Magenta_Biolegend_102515.jpg) |
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28 changes: 28 additions & 0 deletions docs/supporting_material/CD4_AF647/0000-0002-6944-6997.md
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---
layout: default
---

# Configurations

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------|
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BioLegend | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BioLegend | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Spleen | Healthy | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) |

# Publications



# Additional Notes

<a name="notes"></a>
1. Dilution 1:50.

| Mouse Brain Tumor Hochest (Blue) mCherry (green, Novus #NBP2-25158 ) CD4 (Magenta, Biolegend #100533) |
|:-------:|
| ![](Mouse_Tumor_Brain_Hochest_Blue_mCherry_green_Novus_NBP2_25158_CD4_Magenta_Biolegend_100533.jpg) |


| Mouse Spleen Hochest (Blue) CD4 (Magenta Biolegend #100533) |
|:-------:|
| ![](Mouse_Spleen_Hochest_Blue_CD4_Magenta_Biolegend_100533.jpg) |
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22 changes: 22 additions & 0 deletions docs/supporting_material/CXCL12_NA/0000-0002-6944-6997.md
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---
layout: default
---

# Configurations

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------|:-----------------|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------|
| P63158 | Primary Antibody | CXCL12 | Mouse | Rabbit | IgG | polyclonal | Abcam | AB25117 | NA | AB_2088164 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) |

# Publications



# Additional Notes

<a name="notes"></a>
1. Dilution 1:100.

| Mouse Brain Tumor Hochest (Blue) CXCL12 (Green Abcam #AB25117) mCherry (Magenta, Novus #NBP2-25158) |
|:-------:|
| ![](Mouse_Tumor_Brain_Hochest_Blue_CXCL12_Green_Abcam_AB25117_mCherry_Magenta_Novus_NBP2_25158.jpg) |
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22 changes: 22 additions & 0 deletions docs/supporting_material/CXCR4_NA/0000-0002-6944-6997.md
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---
layout: default
---

# Configurations

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:------------------|:-----------------|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------|
| P70658 | Primary Antibody | CXCR4 | Mouse | Rabbit | IgG | polyclonal | Novus Biologicals | NB-100 74396 | NA | AB_1048484 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) |

# Publications



# Additional Notes

<a name="notes"></a>
1. Dilution 1:200.

| Mouse Brain Tumor Hochest (Blue) CXCR4 (Green Novus # NB-100 74396) |
|:-------:|
| ![](Mouse_Brain_Tumor_Hochest_Blue_CXCR4_Green_Novus_NB_100_74396.jpg) |
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22 changes: 22 additions & 0 deletions docs/supporting_material/CXCR7_DyLight_488/0000-0002-6944-6997.md
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---
layout: default
---

# Configurations

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:------------------|:-----------------|:------------|:-------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------|
| P56485 | Primary Antibody | CXCR7 | Mouse | Rabbit | IgG | polyclonal | Novus Biologicals | NBP2-24779G | DyLight 488 | NA | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) |

# Publications



# Additional Notes

<a name="notes"></a>
1. Dilution 1:100.

| Mouse Brain Tumor Hochest (Blue) CXCR7 (Green Novus #NBP2-24779G) |
|:-------:|
| ![](Mouse_Brain_Tumor_Hochest_Blue_CXCR7_Green_Novus_NBP2_24779G.jpg) |
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22 changes: 22 additions & 0 deletions docs/supporting_material/GFAP_eF570/0000-0002-6944-6997.md
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---
layout: default
---

# Configurations

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------|
| P03995 | Primary Antibody | GFAP | Mouse | Mouse | IgG1 | GA5 | Thermo Fisher Scientific | 41-9892-82 | eF570 | AB_2573656 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) |

# Publications



# Additional Notes

<a name="notes"></a>
1. Dilution 1:50.

| Mouse Brain Tumor Hochest (Cyan) GFAP (Orange Thermo #41-9892-82) |
|:-------:|
| ![](Mouse_Brain_Tumor_Hochest_cyan_GFAP_Orange_Thermo_41_9892_82.jpg) |
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