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Merge pull request #102 from zivy/addingReagents
Adding multiple reagent configurations and supporting material.
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docs/supporting_material/CD11c_AF647/0000-0002-6944-6997.md
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# Configurations | ||
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| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | | ||
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:-----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| | ||
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117314 | AF647 | AB_492850 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | | ||
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# Publications | ||
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# Additional Notes | ||
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<a name="notes"></a> | ||
1. Dilution 1:25. | ||
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| Mouse Spleen Hochest (Blue) CD11c (Magenta Biolegend #117314) | | ||
|:-------:| | ||
| ![](Mouse_Spleen_Hochest_Blue_CD11c_Magenta_Biolegend_117314.jpg) | |
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docs/supporting_material/CD31_AF647/0000-0002-6944-6997.md
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layout: default | ||
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# Configurations | ||
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| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | | ||
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| | ||
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102515 | AF647 | AB_2161030 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | | ||
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# Publications | ||
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# Additional Notes | ||
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<a name="notes"></a> | ||
1. Dilution 1:100. | ||
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| Mouse Brain Tumor Hochest(Blue) mCherry (Green Novus #NBP2-25158) CD31(Magenta Biolegend #102515) | | ||
|:-------:| | ||
| ![](Mouse_Brain_Tumor_Hochest_Blue_mCherry_Green_Novus_NBP2_25158_CD31_Magenta_Biolegend_102515.jpg) | |
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layout: default | ||
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# Configurations | ||
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| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | | ||
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| | ||
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BioLegend | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | | ||
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BioLegend | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Spleen | Healthy | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | | ||
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# Publications | ||
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# Additional Notes | ||
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<a name="notes"></a> | ||
1. Dilution 1:50. | ||
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| Mouse Brain Tumor Hochest (Blue) mCherry (green, Novus #NBP2-25158 ) CD4 (Magenta, Biolegend #100533) | | ||
|:-------:| | ||
| ![](Mouse_Tumor_Brain_Hochest_Blue_mCherry_green_Novus_NBP2_25158_CD4_Magenta_Biolegend_100533.jpg) | | ||
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| Mouse Spleen Hochest (Blue) CD4 (Magenta Biolegend #100533) | | ||
|:-------:| | ||
| ![](Mouse_Spleen_Hochest_Blue_CD4_Magenta_Biolegend_100533.jpg) | |
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layout: default | ||
--- | ||
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# Configurations | ||
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| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | | ||
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------|:-----------------|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| | ||
| P63158 | Primary Antibody | CXCL12 | Mouse | Rabbit | IgG | polyclonal | Abcam | AB25117 | NA | AB_2088164 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | | ||
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# Publications | ||
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# Additional Notes | ||
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<a name="notes"></a> | ||
1. Dilution 1:100. | ||
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| Mouse Brain Tumor Hochest (Blue) CXCL12 (Green Abcam #AB25117) mCherry (Magenta, Novus #NBP2-25158) | | ||
|:-------:| | ||
| ![](Mouse_Tumor_Brain_Hochest_Blue_CXCL12_Green_Abcam_AB25117_mCherry_Magenta_Novus_NBP2_25158.jpg) | |
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layout: default | ||
--- | ||
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# Configurations | ||
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| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | | ||
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:------------------|:-----------------|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| | ||
| P70658 | Primary Antibody | CXCR4 | Mouse | Rabbit | IgG | polyclonal | Novus Biologicals | NB-100 74396 | NA | AB_1048484 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | | ||
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# Publications | ||
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# Additional Notes | ||
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<a name="notes"></a> | ||
1. Dilution 1:200. | ||
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| Mouse Brain Tumor Hochest (Blue) CXCR4 (Green Novus # NB-100 74396) | | ||
|:-------:| | ||
| ![](Mouse_Brain_Tumor_Hochest_Blue_CXCR4_Green_Novus_NB_100_74396.jpg) | |
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docs/supporting_material/CXCR7_DyLight_488/0000-0002-6944-6997.md
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Original file line number | Diff line number | Diff line change |
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layout: default | ||
--- | ||
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# Configurations | ||
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| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | | ||
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:------------------|:-----------------|:------------|:-------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| | ||
| P56485 | Primary Antibody | CXCR7 | Mouse | Rabbit | IgG | polyclonal | Novus Biologicals | NBP2-24779G | DyLight 488 | NA | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | | ||
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# Publications | ||
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# Additional Notes | ||
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<a name="notes"></a> | ||
1. Dilution 1:100. | ||
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| Mouse Brain Tumor Hochest (Blue) CXCR7 (Green Novus #NBP2-24779G) | | ||
|:-------:| | ||
| ![](Mouse_Brain_Tumor_Hochest_Blue_CXCR7_Green_Novus_NBP2_24779G.jpg) | |
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docs/supporting_material/GFAP_eF570/0000-0002-6944-6997.md
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layout: default | ||
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# Configurations | ||
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| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | | ||
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| | ||
| P03995 | Primary Antibody | GFAP | Mouse | Mouse | IgG1 | GA5 | Thermo Fisher Scientific | 41-9892-82 | eF570 | AB_2573656 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | | ||
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# Publications | ||
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# Additional Notes | ||
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<a name="notes"></a> | ||
1. Dilution 1:50. | ||
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| Mouse Brain Tumor Hochest (Cyan) GFAP (Orange Thermo #41-9892-82) | | ||
|:-------:| | ||
| ![](Mouse_Brain_Tumor_Hochest_cyan_GFAP_Orange_Thermo_41_9892_82.jpg) | |
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