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Adding reagent validations from Koenig lab, McMaster University.
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zivy committed Jul 2, 2024
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19 changes: 19 additions & 0 deletions data/image_resources.csv
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Expand Up @@ -164,3 +164,22 @@ CD45_PE/Human_Tonsil_FFPE_Hoechst_blue_CD45_PE_green.jpg,"Human tonsil: Hoechst
CD8_AF647/Human_Tonsil_FFPE_Hoechst_blue_CD8_red.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD8 (red, BioLegend cat. no. 372906)",e2a8ea56916b2337125ea81c18050771,"['CD8_AF647/0009-0006-9784-2694.md']"
CTLA4_AF647/Human_Tonsil_FFPE_Hoechst_blue_CTLA4_red.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CTLA4 (red, Abcam cat. no. ab270127)",0319ef9d4863816c1d28cfa5e3ec8258,"['CTLA4_AF647/0009-0006-9784-2694.md']"
PD-1_AF555/Human_Tonsil_FFPE_Hoechst_blue_PD1_green.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and PD-1 (green, Abcam cat. no. ab275126)",32a9ea3243d06baf17be375554c5a9d7,"['PD-1_AF555/0009-0006-9784-2694.md']"
CD23_AF647/CD23_AF647_Grey_Mouse_LN_2_Large.jpg,"Mouse (naive) lymph node: CD23 (grey, catalog number 101611)",3c6684993361eb1aa6a61f6054b2fd2b,['CD23_AF647/0000-0002-8909-5039']
CD23_AF647/CD23_AF647_Grey_Mouse_LN_1_Large.jpg,"Mouse (immunized) lymph node: CD23 (grey, catalog number 101611)",1e9203e92c26bf6b151ad4872c24a7c7,['CD23_AF647/0000-0002-8909-5039']
CD35_BV510/CD35_BV510_Green_DAPI_Blue_Mouse_LN.jpg,"Mouse (immunized) lymph node: CD35 (green, catalog number 740132) and DAPI (blue, catalog number 62248)",284a4ff657463f54944c9bc42c6cebec,['CD35_BV510/0000-0002-1461-0999']
Alpha_smooth_muscle_actin_AF488/aSMA_AF488_Grey_Mouse_SI.jpg,"Mouse small intestine: alpha smooth muscle actin (grey, catalogue number 53-9760-82)",51a470bd7b549f77a5d8616442527b67,['Alpha_smooth_muscle_actin_AF488/0000-0002-1461-0999']
B220_AF647/B220_AF647_Grey_Mouse_LN.jpg,"Mouse lymph node: B220 (grey, catalog number 103226)",594441a0105afe2c0f2d0f078e1301f2,['B220_AF647/0000-0002-1461-0999']
CD11b_PE/CD11b_PE_Grey_Mouse_LN.jpg,"Mouse lymph node: CD11b (grey, catalog number 101217)",fb45af754793db4a66ae930241c7613d,['CD11b_PE/0000-0002-8909-5039']
CD11c_AF488/CD11c_AF488_Grey_Mouse_LN.jpg,"Mouse lymph node: CD11c (grey, catalog number MCD11c20)",ee6003913fbc784cae4f0881658c1ff3,['CD11c_AF488/0000-0002-1461-0999']
CD200_BV421/CD200_BV421_Grey_Mouse_LN.jpg,"Mouse lymph node: CD11c (grey, catalog number 565547)",354c6e657d05a678e0406158f51b294d,['CD200_BV421/0000-0002-1461-0999']
CD3_AF488/CD3_488_Grey_Mouse_LN.jpg,"Mouse lymph node: CD3 (grey, catalog number 100210)",87f4c2106f8e91275784e64caca088b7,['CD3_AF488/0000-0002-1461-0999']
CD31_AF594/CD31_AF594_Grey_Mouse_LN.jpg,"Mouse lymph node: CD31 (grey, catalog number 102520)",e96c4e0587f845e94e34f8e74d72cdd6,['CD31_AF594/0000-0002-1461-0999']
CD4_BV421/CD4_BV421_Grey_Mouse_LN_large.jpg,"Mouse lymph node: CD4 (grey, catalog number 100449)",f7e2ff75a88dc016e7b436dc8e120641,['CD4_BV421/0000-0002-1461-0999']
CD4_BV510/CD4_BV510_Grey_Mouse_LN.jpg,"Mouse lymph node: CD4 (grey, catalog number 100443)",ffaa812ed72823857aed78d747ba0306,['CD4_BV510/0000-0002-1461-0999']
CD45.1_AF488/CD45.1_AF488_Magenta_CD138_AF647_Yellow_Mouse_LN.jpg,"Mouse (immunized) lymph node: CD45.1 (yellow, catalog number 110718) and CD138 (magenta, catalog number 142526)",40018e55494cb00bb37f8f5f1210c83c,['CD45.1_AF488/0000-0002-8909-5039']
CD45_AF594/CD45_AF594_Grey_Mouse_LN.jpg,"Mouse lymph node: CD45 (grey, catalog number 103144)",e8e0fabc8fcf9a6d0dd6d9543a535a89,['CD45_AF594/0000-0002-1461-0999']
CD64_AF647/CD64_AF647_Grey_Mouse_LN.jpg,"Mouse lymph node: CD64 (grey, catalog number 139322)",0a8e934ad6cc1de5091f8710a9ed41a9,['CD64_AF647/0000-0002-1461-0999']
CD81_PE/CD81_PE_Grey_Mouse_LN.jpg,"Mouse lymph node: CD3 (grey, catalog number 104905)",aa8dfb29dc0bf9db5e1cc5dfbc24741f,['CD81_PE/0000-0002-8909-5039']
IgD_BV510/IgD_BV510_Green_B220_AF647_Blue_Mouse_LN.jpg,"Mouse lymph node: IgD (green, catalog number 405723) and B220 (blue, catalog number 103226)",cb40bddb73e073c692e25d967d92c70f,['IgD_BV510/0000-0002-1461-0999']
Ki-67_AF488/Ki67_AF488_Grey_Mouse_LN.jpg,"Mouse lymph node: Ki67 (grey, catalog number 558616)",5638e7e89b73d31bc55b1dece18752fc,['Ki-67_AF488/0000-0002-1461-0999']
Pax5_AF647/Pax5_AF647_Margenta_CD3_AF488_Cyan_Mouse_LN.jpg,"Mouse lymph node: Pax5 (magenta, catalog number 649704) and CD3 (cyan, catalog number 100210)",74a7a3125d784c1bdcc0f84fad9f96dc,['Pax5_AF647/0000-0002-1461-0999']
28 changes: 18 additions & 10 deletions data/reagent_resources.csv

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layout: default
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# Configurations

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------|
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967), [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | |

# Publications



# Additional Notes

<a name="notes"></a>


| Mouse small intestine: alpha smooth muscle actin (grey, catalogue number 53-9760-82) |
|:-------:|
| ![](aSMA_AF488_Grey_Mouse_SI.jpg) |
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| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications), [1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)], [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | |

# Publications
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22 changes: 22 additions & 0 deletions docs/supporting_material/B220_AF647/0000-0002-1461-0999.md
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# Configurations

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------|
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Biolegend | 103226 | AF647 | AB_389330 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | |

# Publications



# Additional Notes

<a name="notes"></a>


| Mouse lymph node: B220 (grey, catalog number 103226) |
|:-------:|
| ![](B220_AF647_Grey_Mouse_LN.jpg) |
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22 changes: 22 additions & 0 deletions docs/supporting_material/CD11b_PE/0000-0002-8909-5039.md
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# Configurations

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------|
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BD Biosciences | 101217 | PE | AB_396680 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) | NA | [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) | |

# Publications



# Additional Notes

<a name="notes"></a>


| Mouse lymph node: CD11b (grey, catalog number 101217) |
|:-------:|
| ![](CD11b_PE_Grey_Mouse_LN.jpg) |
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22 changes: 22 additions & 0 deletions docs/supporting_material/CD11c_AF488/0000-0002-1461-0999.md
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# Configurations

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:-----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:------------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------|
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | Thermo Fisher Scientific | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | |

# Publications



# Additional Notes

<a name="notes"></a>


| Mouse lymph node: CD11c (grey, catalog number MCD11c20) |
|:-------:|
| ![](CD11c_AF488_Grey_Mouse_LN.jpg) |
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Expand Up @@ -6,7 +6,7 @@ layout: default

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:-----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:------------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------|
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | Thermo Fisher Scientific | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | Thermo Fisher Scientific | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) |

# Publications

Expand Down
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22 changes: 22 additions & 0 deletions docs/supporting_material/CD200_BV421/0000-0002-1461-0999.md
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# Configurations

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------|
| Q9ES57 | Primary Antibody | CD200 | Mouse | Rat | IgG2a | OX-90 | BD Biosciences | 565547 | BV421 | AB_2739289 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | |

# Publications



# Additional Notes

<a name="notes"></a>


| Mouse lymph node: CD11c (grey, catalog number 565547) |
|:-------:|
| ![](CD200_BV421_Grey_Mouse_LN.jpg) |
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