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Merge pull request galaxyproject#224 from Delphine-L/VGP8
Addition of workflow Vgp8 : scaffolding with HiC data
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workflows/VGP-assembly-v2/Scaffolding-HiC-VGP8/.dockstore.yml
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version: 1.2 | ||
workflows: | ||
- name: main | ||
primaryDescriptorPath: /Scaffolding-HiC-VGP8.ga | ||
subclass: Galaxy | ||
testParameterFiles: | ||
- /Scaffolding-HiC-VGP8-tests.yml |
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workflows/VGP-assembly-v2/Scaffolding-HiC-VGP8/CHANGELOG.md
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# Changelog | ||
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All notable changes to this project will be documented in this file. | ||
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The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/), | ||
and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). | ||
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## [Unreleased] | ||
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## [0.1] - 2023-09-27 | ||
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Creation of the workflow and tests |
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# Scaffolding HiC | ||
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Scaffolding using HiC data with YAHS | ||
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## Inputs | ||
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1. Scaffolded assembly [fasta] | ||
2. Concatenated HiC forward reads [fastq] | ||
3. Concatenated HiC reverse reads [fastq] | ||
4. Restriction enzyme sequence [txt] | ||
5. Estimated genome size [txt] | ||
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### Outputs | ||
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1. Scaffolds in [fasta] and [gfa] format | ||
2. QC: Assembly statistics | ||
3. QC: Nx plot | ||
4. QC: Size plot | ||
5. QC: BUSCO report | ||
6. QC: Pretext Maps before and after scaffolding |
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workflows/VGP-assembly-v2/Scaffolding-HiC-VGP8/Scaffolding-HiC-VGP8-tests.yml
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- doc: Test outline for Scaffolding-HiC-VGP8 | ||
job: | ||
input GFA: | ||
class: File | ||
location: https://zenodo.org/records/10037496/files/input%20GFA.gfa1?download=1 | ||
filetype: gfa1 | ||
HiC Forward reads: | ||
class: File | ||
location: https://zenodo.org/records/10037496/files/HiC%20Forward%20reads.fastqsanger.gz?download=1 | ||
filetype: fastqsanger.gz | ||
HiC reverse reads: | ||
class: File | ||
location: https://zenodo.org/records/10037496/files/HiC%20reverse%20reads.fastqsanger.gz?download=1 | ||
filetype: fastqsanger.gz | ||
Estimated genome size - Parameter File: | ||
class: File | ||
location: https://zenodo.org/records/10037496/files/Estimated%20genome%20size%20-%20Parameter%20File.txt?download=1 | ||
filetype: txt | ||
Restriction enzymes: arima2 | ||
outputs: | ||
Reconciliated scaffolds: | ||
asserts: | ||
has_n_lines: | ||
n: 168 | ||
Nx Plot: | ||
asserts: | ||
has_size: | ||
value : 47000 | ||
delta: 5000 | ||
Pretext Map After HiC scaffolding: | ||
asserts: | ||
has_size: | ||
value : 893000 | ||
delta: 50000 | ||
Busco Summary: | ||
asserts: | ||
has_text: | ||
text: "C:1.0%[S:1.0%,D:0.0%],F:0.4%,M:98.6%,n:3354" | ||
Yahs scaffolds reconciliated: | ||
asserts: | ||
has_n_lines: | ||
n: 169 | ||
Assembly Statistics for s2: | ||
asserts: | ||
has_line: | ||
line: "# scaffolds 84" |
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