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PratikDJagtap authored Nov 20, 2024
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# Clinical Metaproteomics 3: Verification
In proteomic research, verifying peptides or proteins is essential for ensuring data accuracy and biological relevance. This tutorial continues from the clinical metaproteomics discovery workflow, focusing on validating identified microbial peptides using the PepQuery tool.
In proteomics research, verifying detected peptides is an essential step for reporting accurate peptide detections. This tutorial continues from the clinical metaproteomics discovery workflow, focusing on verifying identified microbial peptides using the PepQuery tool. The PepQuery tool provides an output of verified peptides that can be used to generate a verified protein database that can be used for the next Quantitation workflow.
[https://training.galaxyproject.org/training-material/topics/proteomics/tutorials/clinical-mp-3-verification/tutorial.html](https://training.galaxyproject.org/training-material/topics/proteomics/tutorials/clinical-mp-3-verification/tutorial.html)

## Inputs datasets
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- cRAP

### MSMS files
The MGF files can be accessed via Zenodo.
- `PTRC_Skubitz_Plex2_F10_9Aug19_Rage_Rep-19-06-08.mgf`
- `PTRC_Skubitz_Plex2_F11_9Aug19_Rage_Rep-19-06-08.mgf`
- `PTRC_Skubitz_Plex2_F13_9Aug19_Rage_Rep-19-06-08.mgf`
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