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import numpy as np | ||
from PIL import Image | ||
from pathlib import Path | ||
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from wholeslidedata import WholeSlideImage | ||
from wholeslidedata.accessories.asap.imagewriter import WholeSlideMaskWriter, WholeSlideImageWriter | ||
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def take_closest_number(l, number): | ||
return min(l, key=lambda x: abs(x - number)) | ||
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def take_closest_number_index(l, number): | ||
closest = take_closest_number(l, number) | ||
for ind, val in enumerate(l): | ||
if val==closest: return ind | ||
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def save_normal_image_as_tif(path, out_path, spacing): | ||
""" saves a normal image (png, jpeg) as pyramidal tif """ | ||
img = Image.open(str(path)) | ||
arr = np.array(img).squeeze() | ||
img.close() | ||
save_array_as_image(arr, path=out_path, spacing=spacing) | ||
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def save_array_as_image(arr, path, spacing, tile_size=512): | ||
""" saves an array as pyramidal tif """ | ||
if len(arr.shape)==2: | ||
arr = arr[:,:,None] | ||
writer = WholeSlideMaskWriter() | ||
else: | ||
writer = WholeSlideImageWriter() | ||
shape = arr.shape | ||
writer.write( | ||
path=str(path), spacing=spacing, dimensions=(shape[1], shape[0]), | ||
tile_shape=(tile_size, tile_size), | ||
) | ||
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for col in range(0, shape[1]+tile_size, tile_size): #+tile_size if array not divisible by tile_size | ||
for row in range(0, shape[0]+tile_size, tile_size): | ||
tile = arr[row:row+tile_size, col:col+tile_size] | ||
if len(tile)==0: continue #for the edge-case | ||
if tile.shape[0]!=tile_size or tile.shape[1]!=tile_size: | ||
pad = ((0, tile_size-tile.shape[0]),(0, tile_size-tile.shape[1]),(0,0)) | ||
tile = np.pad(tile, pad, mode='constant') | ||
writer.write_tile(tile=tile, coordinates=(col,row)) #col,row (x,y) | ||
writer.save() | ||
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def save_normal_image_as_mask(path, slide_path, out_path): | ||
""" saves an image-mask (png, jpeg) as pyramidal tif for a given slide """ | ||
reader = WholeSlideImage(str(slide_path), backend='asap') | ||
print(zip(reader.spacings, reader.shapes)) | ||
spacings = reader.spacings | ||
shapes = reader.shapes | ||
reader.close() | ||
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mask = Image.open(str(path)) | ||
mask_arr = np.array(mask) | ||
h,w = mask.height, mask.width | ||
mask.close() | ||
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heights = [hw[0] for hw in shapes] | ||
level = take_closest_number_index(heights, h) | ||
print('for mask shape(%d,%d) closest level %d with spacing %.3f, shape %s' % \ | ||
(h, w, level, spacings[level], str(shapes[level]))) | ||
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spacing = spacings[level] | ||
return save_array_as_image(mask_arr, spacing=spacing, path=out_path) | ||
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