Handle removed replisomes in superhelical density calculations #269
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If a replisome is unexpectedly removed from the simulation, the superhelical density calculation will fail (see gh-268). While our simulation does not allow this to happen, it can happen in real cells (see https://doi.org/10.1016/S0968-0004(00)01565-6), so I figured I might as well try to tackle the issue.
My solution was to always assume that the now bare replication fork is located at the last recorded location for a removed replisome. For now, I also assume that the bare fork acts like a regular one, treating it as a stationary boundary for superhelical density calculations. In the future, we could introduce more dynamic models of replication fork collapse that include things like dsDNA breaks and replication restart. At that time, we could replace the placeholder indices for removed replisomes with designated indices for each possibility. We could then calculate superhelical density differently for segments bounded by molecules with those indices.
Other minor things:
runscripts/workflow.py --resume
copy
to allow caching (no need to rerun when resuming)pre-commit
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