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Update README.md
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dfornika authored Feb 20, 2024
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Expand Up @@ -6,6 +6,24 @@ A pipeline for high-quality plasmid assemblies.

Optionally annotate genes. Collects quality info on both incoming and outgoing datasets.

```mermaid
flowchart TD
short_reads --> fastp(fastp)
fastp -- trimmed_short_reads --> plassembler(plassembler)
long_reads --> filtlong(filtlong)
filtlong -- filtered_long_reads --> plassembler
plassembler --> assembly[assembly]
assembly --> prokka(prokka*)
assembly --> bakta(bakta*)
assembly --> quast(quast)
assembly --> bandage(bandage)
prokka --> prokka_annotated_assembly
bakta --> bakta_annotated_assembly
quast --> assembly_qc
bandage --> assembly_diagram
```
\*Optional processes

## Analyses

* Read trimming & QC: [fastp](https://github.com/OpenGene/fastp) and [filtlong](https://github.com/rrwick/Filtlong)
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