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linting of test_assay_growth_curve_chlamy.py
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David Mets committed Mar 8, 2024
1 parent 8a64426 commit 617a64f
Showing 1 changed file with 37 additions and 36 deletions.
73 changes: 37 additions & 36 deletions test_assay_growth_curve_chlamy.py
Original file line number Diff line number Diff line change
@@ -1,59 +1,60 @@

import sys as sys
import time as tm

import cam_tools as ct
import matplotlib.pyplot as plt
import serial as ser
import time as tm

'''This is a script that highlights most of the functions available in cam_tools.py.
"""This is a script that highlights most of the functions available in cam_tools.py.
It connects to the microcontroller, sets up the camera, turns on the grow lights,
allows the use of a barcode reader, turns on the LEDs that are useful for Chlamydomonas
allows the use of a barcode reader, turns on the LEDs that are useful for Chlamydomonas
to grow ("grow lights"), then acquires an image using transillumination once an hour
for 48 hours'''
for 48 hours"""

dev=ser.Serial('/dev/ttyACM0') # establish serial communication with microcontroller
dev = ser.Serial("/dev/ttyACM0") # establish serial communication with microcontroller

dev.write(b'SET LED_TRANS_STATUS 1;') # turn off the LED
dev.write(b"SET LED_TRANS_STATUS 1;") # turn off the LED

if not ct.detect_cams(): # make sure there are blackfly cameras attached to the computer
sys.exit()
sys.exit()

system = ct.ps.System.GetInstance() #start the communication to the cameras
system = ct.ps.System.GetInstance() # start the communication to the cameras

cam_list=system.GetCameras() #get the handles of the cameras
cam_list = system.GetCameras() # get the handles of the cameras

cam=cam_list[0] #set a handle for a specific camera
cam = cam_list[0] # set a handle for a specific camera

cam.Init() #initialze the camera
cam.Init() # initialze the camera

dev.write(b'SET LED_TRANS_STATUS 0;') # turn on the led
dev.write(b'SET LED_460_STATUS 0;') #turn on grow light
dev.write(b'SET LED_670_STATUS 0;') #turn on grow light
dev.write(b"SET LED_TRANS_STATUS 0;") # turn on the led
dev.write(b"SET LED_460_STATUS 0;") # turn on grow light
dev.write(b"SET LED_670_STATUS 0;") # turn on grow light

bcode = ct.bcode_read() #get barcode from usb barcode reader
bcode = ct.bcode_read() # get barcode from usb barcode reader

input('hit enter to start assay') #start the assay
input("hit enter to start assay") # start the assay

for n in range(0,48):
ct.set_expos_time(cam,500) #set the exposure time
dev.write(b'SET LED_460_STATUS 1;') #turn off grow light
dev.write(b'SET LED_670_STATUS 1;') #turn off grow light
dev.write(b'SET LED_TRANS_STATUS 0;') #turn on the led
tm.sleep(5) #wait 5 seconds
images,timestamps = ct.grab_images(cam, n_frames=1) #get images and timestamps for a vide of 10 seconds length from the camera
ct.save_avi(images,barcode=bcode,prefix='algae_growth_curve') # save images
dev.write(b'SET LED_460_STATUS 0;') #turn on grow light
dev.write(b'SET LED_670_STATUS 0;') #turn on grow light
tm.sleep(60*60) #wait 1 hr
dev.write(b'SET LED_TRANS_STATUS 1;') #turn off LED
for _n in range(0, 48):
ct.set_expos_time(cam, 500) # set the exposure time
dev.write(b"SET LED_460_STATUS 1;") # turn off grow light
dev.write(b"SET LED_670_STATUS 1;") # turn off grow light
dev.write(b"SET LED_TRANS_STATUS 0;") # turn on the led
tm.sleep(5) # wait 5 seconds
images, timestamps = ct.grab_images(
cam, n_frames=1
) # get images and timestamps for a vide of 10 seconds length from the camera
ct.save_avi(images, barcode=bcode, prefix="algae_growth_curve") # save images
dev.write(b"SET LED_460_STATUS 0;") # turn on grow light
dev.write(b"SET LED_670_STATUS 0;") # turn on grow light
tm.sleep(60 * 60) # wait 1 hr
dev.write(b"SET LED_TRANS_STATUS 1;") # turn off LED

print(str(timestamps[-1]-timestamps[0])) #print the total imaging time
print(str(timestamps[-1]-timestamps[-2])) #print the time between frames
print(str(timestamps[-1] - timestamps[0])) # print the total imaging time
print(str(timestamps[-1] - timestamps[-2])) # print the time between frames

dev.write(b'SET LED_TRANS_STATUS 1;') #turn off the led
dev.write(b"SET LED_TRANS_STATUS 1;") # turn off the led

del cam #turn off the camera
del cam # turn off the camera

cam_list.Clear() # de-initialize the cameras
cam_list.Clear() # de-initialize the cameras

system.ReleaseInstance() #release the handlers
system.ReleaseInstance() # release the handlers

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