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I think the current steps:
bwa-mem2 \ mem \ -t ${task.cpus} \ -M \ ${alt_aware_option} \ -R \"@RG\\tID:${header.read_group_identifier}.Seq${header.lane}\\tCN:${header.sequencing_center}\\tLB:${header.library_identifier}\\tPL:${header.platform_technology}\\tPU:${header.platform_unit}\\tSM:${header.sample}\" \ ${ref_fasta} \ ${read1_fastq} \ ${read2_fastq} | \ samtools \ view \ -@ ${task.cpus} \ -S \ -b > \ ${lane_level_bam} """ }
followed by
samtools sort \ -@ ${task.cpus} \ -O bam \ -o ${bam_output_filename} \ ${sort_order} \ ${input_bam}
could be replaced with a single process like this:
bwa-mem2 \ mem \ -t ${task.cpus} \ -M \ ${alt_aware_option} \ -R \"@RG\\tID:${header.read_group_identifier}.Seq${header.lane}\\tCN:${header.sequencing_center}\\tLB:${header.library_identifier}\\tPL:${header.platform_technology}\\tPU:${header.platform_unit}\\tSM:${header.sample}\" \ ${ref_fasta} \ ${read1_fastq} \ ${read2_fastq} | \ samtools sort \ -@ ${task.cpus} \ -o ${bam_output_filename} \ -
The text was updated successfully, but these errors were encountered:
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I think the current steps:
followed by
could be replaced with a single process like this:
The text was updated successfully, but these errors were encountered: