diff --git a/chemistry.json b/chemistry.json index 13fc7c5..b484662 100644 --- a/chemistry.json +++ b/chemistry.json @@ -4,7 +4,7 @@ "bc_cut": "", "R1": "[WHITELIST_V3,\"--soloUMIlen 12 --clip5pNbases 58 0 --soloCBstart 1 --soloCBlen 16 --soloUMIstart 17\"]", "R2": "[WHITELIST_V3,\"--soloUMIlen 12\"]", - "paired": "--alignEndsProtrude 58 ConcordantPair" + "paired": "[\"--alignEndsProtrude 58 ConcordantPair\"]" }, "chromiumV3UG": { "length": "58", @@ -16,34 +16,34 @@ "bc_cut": "", "R1": "[WHITELIST_V2,\"--soloUMIlen 10 --clip5pNbases 56 0 --soloCBstart 1 --soloCBlen 16 --soloUMIstart 17\"]", "R2": "[WHITELIST_V2,\"--soloUMIlen 10\"]", - "paired": "--alignEndsProtrude 56 ConcordantPair" + "paired": "[\"--alignEndsProtrude 56 ConcordantPair\"]" }, "dropseq": { "length": "50", "bc_cut": "", "R1": "[\"None --soloUMIlen 8 --clip5pNbases 50 0 --soloCBstart 1 --soloCBlen 12 --soloUMIstart 13\"]", "R2": "[\"None --soloUMIlen 8 --soloCBstart 1 --soloCBlen 12 --soloUMIstart 13\"]", - "paired": "--alignEndsProtrude 50 ConcordantPair" + "paired": "[\"--alignEndsProtrude 50 ConcordantPair\"]" }, "microwellseq": { "length": "54", "bc_cut": "CGACTCACTACAGGG...TCGGTGACACGATCG", "R1": "[\"None --soloUMIlen 6 --clip5pNbases 54 0 --soloCBstart 1 --soloCBlen 18 --soloUMIstart 19\"]", "R2": "[\"None --soloUMIlen 6 --soloCBstart 1 --soloCBlen 18 --soloUMIstart 19\"]", - "paired": "--alignEndsProtrude 54 ConcordantPair" + "paired": "[\"--alignEndsProtrude 54 ConcordantPair\"]" }, "bd": { "length": "53", "bc_cut": "ACTGGCCTGCGA...GGTAGCGGTGACA", "R1": "[\"None --soloUMIlen 8 --clip5pNbases 53 0 --soloCBstart 1 --soloCBlen 27 --soloUMIstart 28\"]", "R2": "[\"None --soloUMIlen 8 --soloCBstart 1 --soloCBlen 27 --soloUMIstart 28\"]", - "paired": "--alignEndsProtrude 53 ConcordantPair" + "paired": "[\"--alignEndsProtrude 53 ConcordantPair\"]" }, "indrop": { "length": "32", "bc_cut": "", "R1": "[\"None --soloUMIlen 6 --clip5pNbases 32 0 --soloCBstart 1 --soloCBlen 8 --soloUMIstart 9\"]", "R2": "[\"None --soloUMIlen 6 --soloCBstart 1 --soloCBlen 8 --soloUMIstart 9\"]", - "paired": "--alignEndsProtrude 32 ConcordantPair" + "paired": "[\"--alignEndsProtrude 32 ConcordantPair\"]" } } diff --git a/rules/cutadapt_star.snake b/rules/cutadapt_star.snake index 43894a2..cfaecb4 100644 --- a/rules/cutadapt_star.snake +++ b/rules/cutadapt_star.snake @@ -239,7 +239,7 @@ rule starsolo_paired: --readFilesCommand gunzip -c \ --runThreadN 12 \ --soloCBwhitelist {params.chemistry} \ - {star_args_paired} \ + {params.star_args_paired} \ --soloBarcodeMate 1 \ --outFilterMultimapNmax 1 \ --outFilterMismatchNmax 999 \