From 5e7646383bf516327fe57d034359cfe82a97d87b Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Fri, 5 Jan 2024 08:39:37 +0000 Subject: [PATCH 001/111] Set use_work_dir_as_temp to true when running at the Sanger --- .github/workflows/sanger_test.yml | 1 + conf/test_full.config | 3 +++ 2 files changed, 4 insertions(+) diff --git a/.github/workflows/sanger_test.yml b/.github/workflows/sanger_test.yml index e69af1e..041c3d9 100644 --- a/.github/workflows/sanger_test.yml +++ b/.github/workflows/sanger_test.yml @@ -18,6 +18,7 @@ jobs: parameters: | { "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/${{ github.repository }}/results-${{ github.sha }}", + "use_work_dir_as_temp": true, } profiles: test,sanger,singularity,cleanup diff --git a/conf/test_full.config b/conf/test_full.config index da747a1..562b16d 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -19,4 +19,7 @@ params { // Fasta references fasta = "/lustre/scratch124/tol/projects/darwin/data/insects/Polyommatus_icarus/assembly/release/ilPolIcar1.1/insdc/GCA_937595015.1.fasta.gz" + + // Need to be set to avoid overfilling /tmp + use_work_dir_as_temp = true } From c76d9a997b5ba2a033c237e0abb6ad57246d7614 Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Fri, 9 Feb 2024 15:36:49 +0000 Subject: [PATCH 002/111] Sort the BAM files before merging to make the output reproducible --- modules/nf-core/samtools/merge/main.nf | 7 ++++++- 1 file changed, 6 insertions(+), 1 deletion(-) diff --git a/modules/nf-core/samtools/merge/main.nf b/modules/nf-core/samtools/merge/main.nf index b73b7cb..5fb8ede 100644 --- a/modules/nf-core/samtools/merge/main.nf +++ b/modules/nf-core/samtools/merge/main.nf @@ -26,6 +26,11 @@ process SAMTOOLS_MERGE { def args = task.ext.args ?: '' prefix = task.ext.prefix ?: "${meta.id}" def file_type = input_files instanceof List ? input_files[0].getExtension() : input_files.getExtension() + if (input_files instanceof List) { + sorted_input_files = input_files.toSorted({it.name}).join(' ') + } else { + sorted_input_files = input_files + } def reference = fasta ? "--reference ${fasta}" : "" """ samtools \\ @@ -34,7 +39,7 @@ process SAMTOOLS_MERGE { $args \\ ${reference} \\ ${prefix}.${file_type} \\ - $input_files + $sorted_input_files cat <<-END_VERSIONS > versions.yml "${task.process}": From 15ad23dc071414d3dc31dc13d775f09e440ef4a4 Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Thu, 25 Jan 2024 15:36:53 +0000 Subject: [PATCH 003/111] Mistletoe (96Gbp, 2.4 M reads) takes 210 GB RAM and slightly over 2 hours --- conf/base.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/base.config b/conf/base.config index bfd327b..6a37a76 100644 --- a/conf/base.config +++ b/conf/base.config @@ -91,7 +91,7 @@ process { withName: MINIMAP2_ALIGN { cpus = { log_increase_cpus(4, 2*task.attempt, meta.read_count/1000000, 2) } - memory = { check_max( (6.GB * Math.ceil( reference.size() / 1000000000 ) + 4.GB * Math.ceil( meta.read_count / 1000000 )) * task.attempt, 'memory' ) } + memory = { check_max( 14.GB * Math.ceil( Math.pow(reference.size() / 1000000000, 0.6)) * task.attempt, 'memory' ) } time = { check_max( 3.h * Math.ceil( meta.read_count / 1000000 ) * task.attempt, 'time' ) } } From e370b8c3d7e8f8653e50cf7fd3ef655db9dfd31d Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Thu, 25 Jan 2024 16:00:31 +0000 Subject: [PATCH 004/111] Created a local module to convert to Fasta without intermediate files --- conf/base.config | 8 +-- conf/modules.config | 3 +- modules.json | 10 ---- modules/local/samtools_collatetofasta.nf | 45 +++++++++++++++++ modules/nf-core/samtools/collate/main.nf | 46 ----------------- modules/nf-core/samtools/collate/meta.yml | 44 ---------------- modules/nf-core/samtools/fasta/main.nf | 44 ---------------- modules/nf-core/samtools/fasta/meta.yml | 61 ----------------------- subworkflows/local/filter_pacbio.nf | 20 ++------ 9 files changed, 52 insertions(+), 229 deletions(-) create mode 100644 modules/local/samtools_collatetofasta.nf delete mode 100644 modules/nf-core/samtools/collate/main.nf delete mode 100644 modules/nf-core/samtools/collate/meta.yml delete mode 100644 modules/nf-core/samtools/fasta/main.nf delete mode 100644 modules/nf-core/samtools/fasta/meta.yml diff --git a/conf/base.config b/conf/base.config index 6a37a76..5ea996e 100644 --- a/conf/base.config +++ b/conf/base.config @@ -24,16 +24,12 @@ process { time = { check_max( 1.hour * task.attempt, 'time' ) } } - withName: 'SAMTOOLS_(FASTA)' { - time = { check_max( 2.hour * task.attempt, 'time' ) } - } - withName: 'SAMTOOLS_(STATS)' { // Actually less than 1 hour for PacBio HiFi data, but confirmed 3 hours for Hi-C time = { check_max( 4.hour * task.attempt, 'time' ) } } - withName: 'SAMTOOLS_(COLLATE|FASTQ|FIXMATE|FLAGSTAT|MARKDUP|MERGE|SORT|VIEW)' { + withName: 'SAMTOOLS_(COLLATETOFASTA|FASTQ|FIXMATE|FLAGSTAT|MARKDUP|MERGE|SORT|VIEW)' { time = { check_max( 8.hour * task.attempt, 'time' ) } } @@ -48,7 +44,7 @@ process { memory = { check_max( 2.GB * task.attempt, 'memory' ) } } - withName: '.*:FILTER_PACBIO:SAMTOOLS_COLLATE' { + withName: 'SAMTOOLS_COLLATETOFASTA' { cpus = { log_increase_cpus(4, 2*task.attempt, 1, 2) } memory = { check_max( 1.GB * Math.ceil( meta.read_count / 1000000 ) * task.attempt, 'memory' ) } } diff --git a/conf/modules.config b/conf/modules.config index 31f4c6b..63f071d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -32,9 +32,8 @@ process { ext.prefix = { "${meta.id}.merge" } } - withName: SAMTOOLS_COLLATE { + withName: SAMTOOLS_COLLATETOFASTA { ext.args = { (params.use_work_dir_as_temp ? "-T." : "") } - ext.prefix = { "${meta.id}.collate" } } withName: BLAST_BLASTN { diff --git a/modules.json b/modules.json index a9a06be..66a383a 100644 --- a/modules.json +++ b/modules.json @@ -41,21 +41,11 @@ "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", "installed_by": ["modules"] }, - "samtools/collate": { - "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] - }, "samtools/faidx": { "branch": "master", "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe", "installed_by": ["modules"] }, - "samtools/fasta": { - "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] - }, "samtools/fastq": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", diff --git a/modules/local/samtools_collatetofasta.nf b/modules/local/samtools_collatetofasta.nf new file mode 100644 index 0000000..81119f2 --- /dev/null +++ b/modules/local/samtools_collatetofasta.nf @@ -0,0 +1,45 @@ +process SAMTOOLS_COLLATETOFASTA { + tag "$meta.id" + label 'process_medium' + + conda "bioconda::samtools=1.17" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : + 'biocontainers/samtools:1.17--h00cdaf9_0' }" + + input: + tuple val(meta), path(input) + + output: + tuple val(meta), path("*.fasta"), emit: fasta + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + + def prefix = task.ext.prefix ?: "${meta.id}" + """ + samtools collate \\ + $args \\ + -O \\ + -u \\ + -T ${prefix}.collate \\ + --threads $task.cpus \\ + ${input} \\ + | \\ + samtools fasta \\ + $args2 \\ + --threads $task.cpus \\ + -0 ${prefix}.fasta \\ + > /dev/null + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/samtools/collate/main.nf b/modules/nf-core/samtools/collate/main.nf deleted file mode 100644 index b23246b..0000000 --- a/modules/nf-core/samtools/collate/main.nf +++ /dev/null @@ -1,46 +0,0 @@ -process SAMTOOLS_COLLATE { - tag "$meta.id" - label 'process_medium' - - conda "bioconda::samtools=1.17" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0': - 'biocontainers/samtools:1.17--h00cdaf9_0' }" - - input: - tuple val(meta), path(input) - path fasta - - output: - tuple val(meta), path("*.bam"), emit: bam, optional: true - tuple val(meta), path("*.cram"), emit: cram, optional: true - tuple val(meta), path("*.sam"), emit: sam, optional: true - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def reference = fasta ? "--reference ${fasta}" : "" - def extension = args.contains("--output-fmt sam") ? "sam" : - args.contains("--output-fmt bam") ? "bam" : - args.contains("--output-fmt cram") ? "cram" : - "bam" - if ("$input" == "${prefix}.${extension}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" - """ - samtools \\ - collate \\ - $args \\ - ${reference} \\ - -@ $task.cpus \\ - -o ${prefix}.${extension} \\ - $input - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') - END_VERSIONS - """ -} diff --git a/modules/nf-core/samtools/collate/meta.yml b/modules/nf-core/samtools/collate/meta.yml deleted file mode 100644 index 0e78403..0000000 --- a/modules/nf-core/samtools/collate/meta.yml +++ /dev/null @@ -1,44 +0,0 @@ -name: "samtools_collate" -description: shuffles and groups reads together by their names -keywords: - - collate - - bam -tools: - - "samtools": - description: "Tools for dealing with SAM, BAM and CRAM files" - homepage: "http://www.htslib.org" - documentation: "https://www.htslib.org/doc/samtools-collate.html" - tool_dev_url: "https://github.com/samtools/samtools" - doi: "10.1093/bioinformatics/btp352" - licence: "['MIT']" - -input: - # Only when we have meta - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - input: - type: file - description: BAM/CRAM/SAM file - pattern: "*.{bam,cram,sam}" - -output: - #Only when we have meta - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - - output: - type: file - description: Collated BAM/CRAM/SAM file - pattern: "*.{bam,cram,sam}" - -authors: - - "@priyanka-surana" diff --git a/modules/nf-core/samtools/fasta/main.nf b/modules/nf-core/samtools/fasta/main.nf deleted file mode 100644 index 3145965..0000000 --- a/modules/nf-core/samtools/fasta/main.nf +++ /dev/null @@ -1,44 +0,0 @@ -process SAMTOOLS_FASTA { - tag "$meta.id" - label 'process_low' - - conda "bioconda::samtools=1.17" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" - - input: - tuple val(meta), path(input) - val(interleave) - - output: - tuple val(meta), path("*_{1,2}.fasta.gz") , optional:true, emit: fasta - tuple val(meta), path("*_interleaved.fasta.gz"), optional:true, emit: interleaved - tuple val(meta), path("*_singleton.fasta.gz") , optional:true, emit: singleton - tuple val(meta), path("*_other.fasta.gz") , optional:true, emit: other - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def output = ( interleave && ! meta.single_end ) ? "> ${prefix}_interleaved.fasta.gz" : - meta.single_end ? "-1 ${prefix}_1.fasta.gz -s ${prefix}_singleton.fasta.gz" : - "-1 ${prefix}_1.fasta.gz -2 ${prefix}_2.fasta.gz -s ${prefix}_singleton.fasta.gz" - """ - samtools \\ - fasta \\ - $args \\ - --threads ${task.cpus-1} \\ - -0 ${prefix}_other.fasta.gz \\ - $input \\ - $output - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' )) - END_VERSIONS - """ -} diff --git a/modules/nf-core/samtools/fasta/meta.yml b/modules/nf-core/samtools/fasta/meta.yml deleted file mode 100644 index 8e45986..0000000 --- a/modules/nf-core/samtools/fasta/meta.yml +++ /dev/null @@ -1,61 +0,0 @@ -name: "samtools_fasta" -description: Converts a SAM/BAM/CRAM file to FASTA -keywords: - - bam - - sam - - cram - - fasta -tools: - - "samtools": - description: "Tools for dealing with SAM, BAM and CRAM files" - homepage: "http://www.htslib.org" - documentation: "https://www.htslib.org/doc/samtools-fasta.html" - tool_dev_url: "https://github.com/samtools/samtools" - doi: "10.1093/bioinformatics/btp352" - licence: "['MIT']" - -input: - # Only when we have meta - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - input: - type: file - description: BAM/CRAM/SAM file - pattern: "*.{bam,cram,sam}" - - interleave: - type: boolean - description: Set true for interleaved fasta files - -output: - #Only when we have meta - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - - fasta: - type: file - description: Compressed FASTA file(s) with reads with either the READ1 or READ2 flag set in separate files. - pattern: "*_{1,2}.fasta.gz" - - interleaved: - type: file - description: Compressed FASTA file with reads with either the READ1 or READ2 flag set in a combined file. Needs collated input file. - pattern: "*_interleaved.fasta.gz" - - singleton: - type: file - description: Compressed FASTA file with singleton reads - pattern: "*_singleton.fasta.gz" - - other: - type: file - description: Compressed FASTA file with reads with either both READ1 and READ2 flags set or unset - pattern: "*_other.fasta.gz" - -authors: - - "@priyanka-surana" diff --git a/subworkflows/local/filter_pacbio.nf b/subworkflows/local/filter_pacbio.nf index 7dfe8a1..818534e 100644 --- a/subworkflows/local/filter_pacbio.nf +++ b/subworkflows/local/filter_pacbio.nf @@ -4,9 +4,7 @@ // include { SAMTOOLS_VIEW as SAMTOOLS_CONVERT } from '../../modules/nf-core/samtools/view/main' -include { SAMTOOLS_COLLATE } from '../../modules/nf-core/samtools/collate/main' -include { SAMTOOLS_FASTA } from '../../modules/nf-core/samtools/fasta/main' -include { GUNZIP } from '../../modules/nf-core/gunzip/main' +include { SAMTOOLS_COLLATETOFASTA } from '../../modules/local/samtools_collatetofasta' include { BLAST_BLASTN } from '../../modules/nf-core/blast/blastn/main' include { PACBIO_FILTER } from '../../modules/local/pacbio_filter' include { SAMTOOLS_VIEW as SAMTOOLS_FILTER } from '../../modules/nf-core/samtools/view/main' @@ -33,22 +31,12 @@ workflow FILTER_PACBIO { // Collate BAM file to create interleaved FASTA - SAMTOOLS_COLLATE ( SAMTOOLS_CONVERT.out.bam, [] ) - ch_versions = ch_versions.mix ( SAMTOOLS_COLLATE.out.versions.first() ) - - - // Convert BAM to FASTA - SAMTOOLS_FASTA ( SAMTOOLS_COLLATE.out.bam, true ) - ch_versions = ch_versions.mix ( SAMTOOLS_FASTA.out.versions.first() ) - - - // Gunzip FASTA file to BLAST - GUNZIP ( SAMTOOLS_FASTA.out.other ) - ch_versions = ch_versions.mix ( GUNZIP.out.versions.first() ) + SAMTOOLS_COLLATETOFASTA ( SAMTOOLS_CONVERT.out.bam ) + ch_versions = ch_versions.mix ( SAMTOOLS_COLLATETOFASTA.out.versions.first() ) // Nucleotide BLAST - BLAST_BLASTN ( GUNZIP.out.gunzip, db ) + BLAST_BLASTN ( SAMTOOLS_COLLATETOFASTA.out.fasta, db ) ch_versions = ch_versions.mix ( BLAST_BLASTN.out.versions.first() ) From 04c160d2bc44d4042c562895436e982087e26d46 Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Thu, 25 Jan 2024 16:35:10 +0000 Subject: [PATCH 005/111] Simplified the topology of the pipeline by instantiating the CONVERT_STATS sub-workflow just once --- conf/base.config | 7 ++---- conf/modules.config | 36 ++--------------------------- subworkflows/local/align_ont.nf | 10 +------- subworkflows/local/align_pacbio.nf | 10 +------- subworkflows/local/align_short.nf | 6 +---- subworkflows/local/markdup_stats.nf | 10 +------- workflows/readmapping.nf | 15 +++++++++++- 7 files changed, 22 insertions(+), 72 deletions(-) diff --git a/conf/base.config b/conf/base.config index 5ea996e..bdb122d 100644 --- a/conf/base.config +++ b/conf/base.config @@ -37,11 +37,8 @@ process { memory = { check_max( 250.MB * task.attempt, 'memory' ) } } - withName: '.*:ALIGN_(HIFI|HIC|ILLUMINA):.*:SAMTOOLS_(STATS|VIEW)' { - memory = { check_max( 1.GB * task.attempt, 'memory' ) } - } - withName: '.*:ALIGN_(CLR|ONT):.*:SAMTOOLS_(STATS|VIEW)' { - memory = { check_max( 2.GB * task.attempt, 'memory' ) } + withName: 'SAMTOOLS_(STATS|VIEW)' { + memory = { check_max( ((meta.datatype == "pacbio_clr" || meta.datatype == "ont") ? 2.GB : 1.GB) * task.attempt, 'memory' ) } } withName: 'SAMTOOLS_COLLATETOFASTA' { diff --git a/conf/modules.config b/conf/modules.config index 63f071d..2390814 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -102,41 +102,9 @@ process { ] } - withName: '.*:ALIGN_HIC:MARKDUP_STATS:CONVERT_STATS:.*' { + withName: '.*:CONVERT_STATS:.*' { publishDir = [ - path: { "${params.outdir}/read_mapping/hic" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*:ALIGN_ILLUMINA:MARKDUP_STATS:CONVERT_STATS:.*' { - publishDir = [ - path: { "${params.outdir}/read_mapping/illumina" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*:ALIGN_HIFI:CONVERT_STATS:.*' { - publishDir = [ - path: { "${params.outdir}/read_mapping/pacbio" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*:ALIGN_CLR:CONVERT_STATS:.*' { - publishDir = [ - path: { "${params.outdir}/read_mapping/pacbio" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*:ALIGN_ONT:CONVERT_STATS:.*' { - publishDir = [ - path: { "${params.outdir}/read_mapping/ont" }, + path: { "${params.outdir}/read_mapping/${meta.datatype}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] diff --git a/subworkflows/local/align_ont.nf b/subworkflows/local/align_ont.nf index c1d2263..cf1feeb 100644 --- a/subworkflows/local/align_ont.nf +++ b/subworkflows/local/align_ont.nf @@ -5,7 +5,6 @@ include { MINIMAP2_ALIGN } from '../../modules/nf-core/minimap2/align/main' include { SAMTOOLS_MERGE } from '../../modules/nf-core/samtools/merge/main' include { SAMTOOLS_SORT } from '../../modules/nf-core/samtools/sort/main' -include { CONVERT_STATS } from '../../subworkflows/local/convert_stats' workflow ALIGN_ONT { @@ -52,15 +51,8 @@ workflow ALIGN_ONT { | map { meta, bam -> [ meta, bam, [] ] } | set { ch_sort } - CONVERT_STATS ( ch_sort, fasta ) - ch_versions = ch_versions.mix ( CONVERT_STATS.out.versions ) - emit: - cram = CONVERT_STATS.out.cram // channel: [ val(meta), /path/to/cram ] - crai = CONVERT_STATS.out.crai // channel: [ val(meta), /path/to/crai ] - stats = CONVERT_STATS.out.stats // channel: [ val(meta), /path/to/stats ] - idxstats = CONVERT_STATS.out.idxstats // channel: [ val(meta), /path/to/idxstats ] - flagstat = CONVERT_STATS.out.flagstat // channel: [ val(meta), /path/to/flagstat ] + bam = ch_sort // channel: [ val(meta), /path/to/bam ] versions = ch_versions // channel: [ versions.yml ] } diff --git a/subworkflows/local/align_pacbio.nf b/subworkflows/local/align_pacbio.nf index 01cd1ac..a29e827 100644 --- a/subworkflows/local/align_pacbio.nf +++ b/subworkflows/local/align_pacbio.nf @@ -5,7 +5,6 @@ include { FILTER_PACBIO } from '../../subworkflows/local/filter_pacbio' include { MINIMAP2_ALIGN } from '../../modules/nf-core/minimap2/align/main' include { SAMTOOLS_MERGE } from '../../modules/nf-core/samtools/merge/main' -include { CONVERT_STATS } from '../../subworkflows/local/convert_stats' workflow ALIGN_PACBIO { @@ -58,15 +57,8 @@ workflow ALIGN_PACBIO { | map { meta, bam -> [ meta, bam, [] ] } | set { ch_sort } - CONVERT_STATS ( ch_sort, fasta ) - ch_versions = ch_versions.mix ( CONVERT_STATS.out.versions ) - emit: - cram = CONVERT_STATS.out.cram // channel: [ val(meta), /path/to/cram ] - crai = CONVERT_STATS.out.crai // channel: [ val(meta), /path/to/crai ] - stats = CONVERT_STATS.out.stats // channel: [ val(meta), /path/to/stats ] - idxstats = CONVERT_STATS.out.idxstats // channel: [ val(meta), /path/to/idxstats ] - flagstat = CONVERT_STATS.out.flagstat // channel: [ val(meta), /path/to/flagstat ] + bam = ch_sort // channel: [ val(meta), /path/to/bam ] versions = ch_versions // channel: [ versions.yml ] } diff --git a/subworkflows/local/align_short.nf b/subworkflows/local/align_short.nf index a6e574b..73a8e2e 100644 --- a/subworkflows/local/align_short.nf +++ b/subworkflows/local/align_short.nf @@ -34,10 +34,6 @@ workflow ALIGN_SHORT { emit: - cram = MARKDUP_STATS.out.cram // channel: [ val(meta), /path/to/cram ] - crai = MARKDUP_STATS.out.crai // channel: [ val(meta), /path/to/crai ] - stats = MARKDUP_STATS.out.stats // channel: [ val(meta), /path/to/stats ] - idxstats = MARKDUP_STATS.out.idxstats // channel: [ val(meta), /path/to/idxstats ] - flagstat = MARKDUP_STATS.out.flagstat // channel: [ val(meta), /path/to/flagstat ] + bam = MARKDUP_STATS.out.bam // channel: [ val(meta), /path/to/bam ] versions = ch_versions // channel: [ versions.yml ] } diff --git a/subworkflows/local/markdup_stats.nf b/subworkflows/local/markdup_stats.nf index 9b271f8..7d30257 100644 --- a/subworkflows/local/markdup_stats.nf +++ b/subworkflows/local/markdup_stats.nf @@ -6,7 +6,6 @@ include { SAMTOOLS_MERGE } from '../../modules/nf-core/samtools/merge/main' include { SAMTOOLS_SORT } from '../../modules/nf-core/samtools/sort/main' include { SAMTOOLS_SORMADUP } from '../../modules/local/samtools_sormadup' -include { CONVERT_STATS } from '../../subworkflows/local/convert_stats' workflow MARKDUP_STATS { @@ -57,15 +56,8 @@ workflow MARKDUP_STATS { | map { meta, bam -> [ meta, bam, [] ] } | set { ch_stat } - CONVERT_STATS ( ch_stat, fasta ) - ch_versions = ch_versions.mix ( CONVERT_STATS.out.versions ) - emit: - cram = CONVERT_STATS.out.cram // channel: [ val(meta), /path/to/cram ] - crai = CONVERT_STATS.out.crai // channel: [ val(meta), /path/to/crai ] - stats = CONVERT_STATS.out.stats // channel: [ val(meta), /path/to/stats ] - idxstats = CONVERT_STATS.out.idxstats // channel: [ val(meta), /path/to/idxstats ] - flagstat = CONVERT_STATS.out.flagstat // channel: [ val(meta), /path/to/flagstat ] + bam = ch_stat // channel: [ val(meta), /path/to/bam ] versions = ch_versions // channel: [ versions.yml ] } diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index 9d12b0b..e579d3e 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -32,6 +32,7 @@ include { ALIGN_SHORT as ALIGN_ILLUMINA } from '../subworkflows/local/align_shor include { ALIGN_PACBIO as ALIGN_HIFI } from '../subworkflows/local/align_pacbio' include { ALIGN_PACBIO as ALIGN_CLR } from '../subworkflows/local/align_pacbio' include { ALIGN_ONT } from '../subworkflows/local/align_ont' +include { CONVERT_STATS } from '../subworkflows/local/convert_stats' /* @@ -126,10 +127,22 @@ workflow READMAPPING { ch_versions = ch_versions.mix ( ALIGN_ONT.out.versions ) + ch_aligned_bams = Channel.empty() + | mix( ALIGN_HIC.out.bam ) + | mix( ALIGN_ILLUMINA.out.bam ) + | mix( ALIGN_HIFI.out.bam ) + | mix( ALIGN_CLR.out.bam ) + | mix( ALIGN_ONT.out.bam ) + CONVERT_STATS ( ch_aligned_bams, PREPARE_GENOME.out.fasta ) + ch_versions = ch_versions.mix ( CONVERT_STATS.out.versions ) + // // MODULE: To compress PacBio HiFi aligned CRAM files // - CRUMBLE ( ALIGN_HIFI.out.cram, [], true ) + CONVERT_STATS.out.cram + | filter { meta, bam -> meta.datatype == "pacbio" } + | set { ch_pacbio_bams } + CRUMBLE ( ch_pacbio_bams, [], true ) ch_versions = ch_versions.mix ( CRUMBLE.out.versions ) From 6565f9314951221760f422776cfedff421cba2e7 Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Thu, 25 Jan 2024 17:04:46 +0000 Subject: [PATCH 006/111] Rolled out the minimap2 -I setting to all data types --- conf/modules.config | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 2390814..aeab190 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -48,7 +48,6 @@ process { ext.prefix = { "${meta.id}.filter" } } - withName: '.*:.*:ALIGN_HIFI:MINIMAP2_ALIGN' { // minimap2 2.24 can only work with genomes up to 4 Gbp. For larger genomes, add the -I option with the genome size in Gbp. // In fact, we can also use -I to *decrease* the memory requirements for smaller genomes // NOTE: minimap2 uses the decimal system ! 1G = 1,000,000,000 bp @@ -56,15 +55,16 @@ process { // NOTE: minimap2 2.25 raises the default to 8G, which means higher memory savings on smaller genomes // NOTE: Use `reference.size()` for now, and switch to `meta2.genome_size` once we update the modules. // ext.args = { "-ax map-hifi --cs=short -R ${meta.read_group} -I" + Math.ceil(meta.genome_size/1e9) + 'G' } + withName: '.*:.*:ALIGN_HIFI:MINIMAP2_ALIGN' { ext.args = { "-ax map-hifi --cs=short -R ${meta.read_group} -I" + Math.ceil(reference.size()/1e9) + 'G' } } withName: '.*:.*:ALIGN_CLR:MINIMAP2_ALIGN' { - ext.args = { "-ax map-pb -R ${meta.read_group}" } + ext.args = { "-ax map-pb -R ${meta.read_group} -I" + Math.ceil(reference.size()/1e9) + 'G' } } withName: '.*:.*:ALIGN_ONT:MINIMAP2_ALIGN' { - ext.args = { "-ax map-ont -R ${meta.read_group}" } + ext.args = { "-ax map-ont -R ${meta.read_group} -I" + Math.ceil(reference.size()/1e9) + 'G' } } withName: '.*:CONVERT_STATS:SAMTOOLS_VIEW' { From b3cb5d8f3c248876ae04e792ceb57f6b1d0eef12 Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Thu, 25 Jan 2024 17:29:24 +0000 Subject: [PATCH 007/111] Combined two consecutive samtools steps to save some space --- conf/base.config | 4 +- conf/modules.config | 4 -- modules/local/samtools_filtertofastq.nf | 57 +++++++++++++++++++++++++ subworkflows/local/filter_pacbio.nf | 18 +++----- 4 files changed, 65 insertions(+), 18 deletions(-) create mode 100644 modules/local/samtools_filtertofastq.nf diff --git a/conf/base.config b/conf/base.config index bdb122d..66b612b 100644 --- a/conf/base.config +++ b/conf/base.config @@ -20,7 +20,7 @@ process { memory = { check_max( 50.MB * task.attempt, 'memory' ) } time = { check_max( 30.min * task.attempt, 'time' ) } - withName: 'SAMTOOLS_(CONVERT|FILTER)' { + withName: 'SAMTOOLS_(CONVERT)' { time = { check_max( 1.hour * task.attempt, 'time' ) } } @@ -29,7 +29,7 @@ process { time = { check_max( 4.hour * task.attempt, 'time' ) } } - withName: 'SAMTOOLS_(COLLATETOFASTA|FASTQ|FIXMATE|FLAGSTAT|MARKDUP|MERGE|SORT|VIEW)' { + withName: 'SAMTOOLS_(COLLATETOFASTA|FILTERTOFASTQ|FIXMATE|FLAGSTAT|MARKDUP|MERGE|SORT|VIEW)' { time = { check_max( 8.hour * task.attempt, 'time' ) } } diff --git a/conf/modules.config b/conf/modules.config index aeab190..3a93825 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -44,10 +44,6 @@ process { ext.args = "-be '[rq]>=0.99' -x fi -x fp -x ri -x rp --write-index" } - withName: SAMTOOLS_FILTER { - ext.prefix = { "${meta.id}.filter" } - } - // minimap2 2.24 can only work with genomes up to 4 Gbp. For larger genomes, add the -I option with the genome size in Gbp. // In fact, we can also use -I to *decrease* the memory requirements for smaller genomes // NOTE: minimap2 uses the decimal system ! 1G = 1,000,000,000 bp diff --git a/modules/local/samtools_filtertofastq.nf b/modules/local/samtools_filtertofastq.nf new file mode 100644 index 0000000..fbee087 --- /dev/null +++ b/modules/local/samtools_filtertofastq.nf @@ -0,0 +1,57 @@ +process SAMTOOLS_FILTERTOFASTQ { + tag "$meta.id" + label 'process_low' + + conda "bioconda::samtools=1.17" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : + 'biocontainers/samtools:1.17--h00cdaf9_0' }" + + input: + tuple val(meta), path(input), path(index) + path qname + + output: + tuple val(meta), path("*.fastq.gz") , emit: fastq + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + samtools view \\ + --threads $task.cpus \\ + --qname-file ${qname} \\ + --unoutput - \\ + $args \\ + -o /dev/null \\ + $input \\ + | \\ + samtools fastq \\ + $args2 \\ + --threads $task.cpus \\ + -0 ${prefix}.fastq.gz \\ + - \\ + > /dev/null + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + echo | gzip > ${prefix}.fastq.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/subworkflows/local/filter_pacbio.nf b/subworkflows/local/filter_pacbio.nf index 818534e..759880c 100644 --- a/subworkflows/local/filter_pacbio.nf +++ b/subworkflows/local/filter_pacbio.nf @@ -7,8 +7,7 @@ include { SAMTOOLS_VIEW as SAMTOOLS_CONVERT } from '../../modules/nf-core/samtoo include { SAMTOOLS_COLLATETOFASTA } from '../../modules/local/samtools_collatetofasta' include { BLAST_BLASTN } from '../../modules/nf-core/blast/blastn/main' include { PACBIO_FILTER } from '../../modules/local/pacbio_filter' -include { SAMTOOLS_VIEW as SAMTOOLS_FILTER } from '../../modules/nf-core/samtools/view/main' -include { SAMTOOLS_FASTQ } from '../../modules/nf-core/samtools/fastq/main' +include { SAMTOOLS_FILTERTOFASTQ } from '../../modules/local/samtools_filtertofastq' workflow FILTER_PACBIO { @@ -45,7 +44,7 @@ workflow FILTER_PACBIO { ch_versions = ch_versions.mix ( PACBIO_FILTER.out.versions.first() ) - // Create filtered BAM file + // Filter the BAM file and convert to FASTQ SAMTOOLS_CONVERT.out.bam | join ( SAMTOOLS_CONVERT.out.csi ) | join ( PACBIO_FILTER.out.list ) @@ -59,16 +58,11 @@ workflow FILTER_PACBIO { | map { meta, bam, csi, list -> list } | set { ch_lists } - SAMTOOLS_FILTER ( ch_reads, [ [], [] ], ch_lists ) - ch_versions = ch_versions.mix ( SAMTOOLS_FILTER.out.versions.first() ) - - - // Convert BAM to FASTQ - SAMTOOLS_FASTQ ( SAMTOOLS_FILTER.out.unoutput, true ) - ch_versions = ch_versions.mix ( SAMTOOLS_FASTQ.out.versions.first() ) + SAMTOOLS_FILTERTOFASTQ ( ch_reads, ch_lists ) + ch_versions = ch_versions.mix ( SAMTOOLS_FILTERTOFASTQ.out.versions.first() ) emit: - fastq = SAMTOOLS_FASTQ.out.other // channel: [ meta, /path/to/fastq ] - versions = ch_versions // channel: [ versions.yml ] + fastq = SAMTOOLS_FILTERTOFASTQ.out.fastq // channel: [ meta, /path/to/fastq ] + versions = ch_versions // channel: [ versions.yml ] } From 3d7a70080727f3e7adb2ce42a7b76a40ff6bea31 Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Thu, 25 Jan 2024 18:02:11 +0000 Subject: [PATCH 008/111] Sort the BAM file directly with bwamem2 to save some disk space --- conf/base.config | 15 +++----- conf/modules.config | 4 --- modules.json | 5 --- modules/nf-core/samtools/sort/main.nf | 49 -------------------------- modules/nf-core/samtools/sort/meta.yml | 48 ------------------------- subworkflows/local/align_ont.nf | 1 - subworkflows/local/align_short.nf | 4 +-- subworkflows/local/markdup_stats.nf | 8 +---- 8 files changed, 7 insertions(+), 127 deletions(-) delete mode 100644 modules/nf-core/samtools/sort/main.nf delete mode 100644 modules/nf-core/samtools/sort/meta.yml diff --git a/conf/base.config b/conf/base.config index 66b612b..aa296e0 100644 --- a/conf/base.config +++ b/conf/base.config @@ -29,7 +29,7 @@ process { time = { check_max( 4.hour * task.attempt, 'time' ) } } - withName: 'SAMTOOLS_(COLLATETOFASTA|FILTERTOFASTQ|FIXMATE|FLAGSTAT|MARKDUP|MERGE|SORT|VIEW)' { + withName: 'SAMTOOLS_(COLLATETOFASTA|FILTERTOFASTQ|FIXMATE|FLAGSTAT|MARKDUP|MERGE|VIEW)' { time = { check_max( 8.hour * task.attempt, 'time' ) } } @@ -52,13 +52,6 @@ process { time = { check_max( 2.h * Math.ceil( meta.read_count / 100000000 ) * task.attempt / log_increase_cpus(2, 6*task.attempt, 1, 2), 'time' ) } } - withName: SAMTOOLS_SORT { - cpus = { log_increase_cpus(4, 2*task.attempt, 1, 2) } - // Memory increases by 768M for each thread - memory = { check_max( 1.GB + 800.MB * log_increase_cpus(4, 2*task.attempt, 1, 2), 'memory' ) } - time = { check_max( 8.hour * Math.ceil( meta.read_count / 1000000000 ) * task.attempt, 'time' ) } - } - withName: BLAST_BLASTN { time = { check_max( 2.hour * Math.ceil( meta.read_count / 1000000 ) * task.attempt, 'time' ) } memory = { check_max( 100.MB + 20.MB * Math.ceil( meta.read_count / 1000000 ) * task.attempt, 'memory' ) } @@ -77,9 +70,9 @@ process { // Runtime for 1 billion reads on 12 threads is a function of the logarithm of the genome size // Runtime is considered proportional to the number of reads and inversely to number of threads time = { check_max( 3.h * task.attempt * Math.ceil(positive_log(meta2.genome_size/100000, 10)) * Math.ceil(meta.read_count/1000000000) * 12 / log_increase_cpus(6, 6*task.attempt, meta.read_count/1000000000, 2), 'time' ) } - // Base RAM usage is about 6 times the genome size. Each thread takes an additional 800 MB RAM - // Memory usage of SAMTOOLS_VIEW is negligible. - memory = { check_max( 6.GB * Math.ceil(meta2.genome_size / 1000000000) + 800.MB * task.attempt * log_increase_cpus(6, 6*task.attempt, meta.read_count/1000000000, 2), 'memory' ) } + // Base RAM usage is about 6 times the genome size. + // Each thread takes an additional 800 MB RAM for bwa-mem2 and 800 MB for samtools sort + memory = { check_max( 8.GB + 6.GB * Math.ceil(meta2.genome_size / 1000000000) + 1600.MB * task.attempt * log_increase_cpus(6, 6*task.attempt, meta.read_count/1000000000, 2), 'memory' ) } } withName: MINIMAP2_ALIGN { diff --git a/conf/modules.config b/conf/modules.config index 3a93825..6259289 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -23,10 +23,6 @@ process { ext.args = { "-R ${meta.read_group}" } } - withName: SAMTOOLS_SORT { - ext.prefix = { "${meta.id}.sort" } - } - withName: SAMTOOLS_MERGE { ext.args = { "-c -p" } ext.prefix = { "${meta.id}.merge" } diff --git a/modules.json b/modules.json index 66a383a..b61ea34 100644 --- a/modules.json +++ b/modules.json @@ -66,11 +66,6 @@ "git_sha": "0460d316170f75f323111b4a2c0a2989f0c32013", "installed_by": ["modules"] }, - "samtools/sort": { - "branch": "master", - "git_sha": "a0f7be95788366c1923171e358da7d049eb440f9", - "installed_by": ["modules"] - }, "samtools/stats": { "branch": "master", "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf deleted file mode 100644 index 2b7753f..0000000 --- a/modules/nf-core/samtools/sort/main.nf +++ /dev/null @@ -1,49 +0,0 @@ -process SAMTOOLS_SORT { - tag "$meta.id" - label 'process_medium' - - conda "bioconda::samtools=1.17" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" - - input: - tuple val(meta), path(bam) - - output: - tuple val(meta), path("*.bam"), emit: bam - tuple val(meta), path("*.csi"), emit: csi, optional: true - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" - """ - samtools sort \\ - $args \\ - -@ $task.cpus \\ - -o ${prefix}.bam \\ - -T $prefix \\ - $bam - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.bam - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') - END_VERSIONS - """ -} diff --git a/modules/nf-core/samtools/sort/meta.yml b/modules/nf-core/samtools/sort/meta.yml deleted file mode 100644 index 0732843..0000000 --- a/modules/nf-core/samtools/sort/meta.yml +++ /dev/null @@ -1,48 +0,0 @@ -name: samtools_sort -description: Sort SAM/BAM/CRAM file -keywords: - - sort - - bam - - sam - - cram -tools: - - samtools: - description: | - SAMtools is a set of utilities for interacting with and post-processing - short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. - These files are generated as output by short read aligners like BWA. - homepage: http://www.htslib.org/ - documentation: http://www.htslib.org/doc/samtools.html - doi: 10.1093/bioinformatics/btp352 - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM/CRAM/SAM file - pattern: "*.{bam,cram,sam}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: Sorted BAM/CRAM/SAM file - pattern: "*.{bam,cram,sam}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - - csi: - type: file - description: BAM index file (optional) - pattern: "*.csi" -authors: - - "@drpatelh" - - "@ewels" diff --git a/subworkflows/local/align_ont.nf b/subworkflows/local/align_ont.nf index cf1feeb..91ed3c7 100644 --- a/subworkflows/local/align_ont.nf +++ b/subworkflows/local/align_ont.nf @@ -4,7 +4,6 @@ include { MINIMAP2_ALIGN } from '../../modules/nf-core/minimap2/align/main' include { SAMTOOLS_MERGE } from '../../modules/nf-core/samtools/merge/main' -include { SAMTOOLS_SORT } from '../../modules/nf-core/samtools/sort/main' workflow ALIGN_ONT { diff --git a/subworkflows/local/align_short.nf b/subworkflows/local/align_short.nf index 73a8e2e..d834f9a 100644 --- a/subworkflows/local/align_short.nf +++ b/subworkflows/local/align_short.nf @@ -23,8 +23,8 @@ workflow ALIGN_SHORT { ch_versions = ch_versions.mix ( SAMTOOLS_FASTQ.out.versions.first() ) - // Align Fastq to Genome - BWAMEM2_MEM ( SAMTOOLS_FASTQ.out.fastq, index, [] ) + // Align Fastq to Genome and output sorted BAM + BWAMEM2_MEM ( SAMTOOLS_FASTQ.out.fastq, index, true ) ch_versions = ch_versions.mix ( BWAMEM2_MEM.out.versions.first() ) diff --git a/subworkflows/local/markdup_stats.nf b/subworkflows/local/markdup_stats.nf index 7d30257..8193ca8 100644 --- a/subworkflows/local/markdup_stats.nf +++ b/subworkflows/local/markdup_stats.nf @@ -4,7 +4,6 @@ // include { SAMTOOLS_MERGE } from '../../modules/nf-core/samtools/merge/main' -include { SAMTOOLS_SORT } from '../../modules/nf-core/samtools/sort/main' include { SAMTOOLS_SORMADUP } from '../../modules/local/samtools_sormadup' @@ -18,13 +17,8 @@ workflow MARKDUP_STATS { ch_versions = Channel.empty() - // Sort BAM file - SAMTOOLS_SORT ( aln ) - ch_versions = ch_versions.mix ( SAMTOOLS_SORT.out.versions.first() ) - - // Collect all BWAMEM2 output by sample name - SAMTOOLS_SORT.out.bam + aln | map { meta, bam -> [['id': meta.id.split('_')[0..-2].join('_'), 'datatype': meta.datatype], meta.read_count, bam] } | groupTuple( by: [0] ) | map { meta, read_counts, bams -> [meta + [read_count: read_counts.sum()], bams] } From 0e21eafd4fe8df1107072b57975aff7d459d550e Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Thu, 25 Jan 2024 18:06:45 +0000 Subject: [PATCH 009/111] Merged the markdup_stats sub-workflow into align_illumina --- subworkflows/local/align_short.nf | 44 ++++++++++++++++++---- subworkflows/local/markdup_stats.nf | 57 ----------------------------- 2 files changed, 37 insertions(+), 64 deletions(-) delete mode 100644 subworkflows/local/markdup_stats.nf diff --git a/subworkflows/local/align_short.nf b/subworkflows/local/align_short.nf index d834f9a..33c27b6 100644 --- a/subworkflows/local/align_short.nf +++ b/subworkflows/local/align_short.nf @@ -2,9 +2,10 @@ // Align short read (HiC and Illumina) data against the genome // -include { SAMTOOLS_FASTQ } from '../../modules/nf-core/samtools/fastq/main' -include { BWAMEM2_MEM } from '../../modules/nf-core/bwamem2/mem/main' -include { MARKDUP_STATS } from '../../subworkflows/local/markdup_stats' +include { SAMTOOLS_FASTQ } from '../../modules/nf-core/samtools/fastq/main' +include { BWAMEM2_MEM } from '../../modules/nf-core/bwamem2/mem/main' +include { SAMTOOLS_MERGE } from '../../modules/nf-core/samtools/merge/main' +include { SAMTOOLS_SORMADUP } from '../../modules/local/samtools_sormadup' workflow ALIGN_SHORT { @@ -28,12 +29,41 @@ workflow ALIGN_SHORT { ch_versions = ch_versions.mix ( BWAMEM2_MEM.out.versions.first() ) - // Merge, markdup, convert, and stats - MARKDUP_STATS ( BWAMEM2_MEM.out.bam, fasta ) - ch_versions = ch_versions.mix ( MARKDUP_STATS.out.versions ) + // Collect all BWAMEM2 output by sample name + BWAMEM2_MEM.out.bam + | map { meta, bam -> [['id': meta.id.split('_')[0..-2].join('_'), 'datatype': meta.datatype], meta.read_count, bam] } + | groupTuple( by: [0] ) + | map { meta, read_counts, bams -> [meta + [read_count: read_counts.sum()], bams] } + | branch { + meta, bams -> + single_bam: bams.size() == 1 + multi_bams: true + } + | set { ch_bams } + + + // Merge, but only if there is more than 1 file + SAMTOOLS_MERGE ( ch_bams.multi_bams, [ [], [] ], [ [], [] ] ) + ch_versions = ch_versions.mix ( SAMTOOLS_MERGE.out.versions.first() ) + + + SAMTOOLS_MERGE.out.bam + | mix ( ch_bams.single_bam ) + | set { ch_bam } + + + // Mark duplicates + SAMTOOLS_SORMADUP ( ch_bam, fasta ) + ch_versions = ch_versions.mix ( SAMTOOLS_SORMADUP.out.versions ) + + + // Convert merged BAM to CRAM and calculate indices and statistics + SAMTOOLS_SORMADUP.out.bam + | map { meta, bam -> [ meta, bam, [] ] } + | set { ch_stat } emit: - bam = MARKDUP_STATS.out.bam // channel: [ val(meta), /path/to/bam ] + bam = ch_stat // channel: [ val(meta), /path/to/bam ] versions = ch_versions // channel: [ versions.yml ] } diff --git a/subworkflows/local/markdup_stats.nf b/subworkflows/local/markdup_stats.nf deleted file mode 100644 index 8193ca8..0000000 --- a/subworkflows/local/markdup_stats.nf +++ /dev/null @@ -1,57 +0,0 @@ -// -// Merge and Markdup all alignments at specimen level -// Convert to CRAM and calculate statistics -// - -include { SAMTOOLS_MERGE } from '../../modules/nf-core/samtools/merge/main' -include { SAMTOOLS_SORMADUP } from '../../modules/local/samtools_sormadup' - - -workflow MARKDUP_STATS { - take: - aln // channel: [ val(meta), /path/to/bam ] - fasta // channel: [ val(meta), /path/to/fasta ] - - - main: - ch_versions = Channel.empty() - - - // Collect all BWAMEM2 output by sample name - aln - | map { meta, bam -> [['id': meta.id.split('_')[0..-2].join('_'), 'datatype': meta.datatype], meta.read_count, bam] } - | groupTuple( by: [0] ) - | map { meta, read_counts, bams -> [meta + [read_count: read_counts.sum()], bams] } - | branch { - meta, bams -> - single_bam: bams.size() == 1 - multi_bams: true - } - | set { ch_bams } - - - // Merge, but only if there is more than 1 file - SAMTOOLS_MERGE ( ch_bams.multi_bams, [ [], [] ], [ [], [] ] ) - ch_versions = ch_versions.mix ( SAMTOOLS_MERGE.out.versions.first() ) - - - SAMTOOLS_MERGE.out.bam - | mix ( ch_bams.single_bam ) - | set { ch_bam } - - - // Mark duplicates - SAMTOOLS_SORMADUP ( ch_bam, fasta ) - ch_versions = ch_versions.mix ( SAMTOOLS_SORMADUP.out.versions ) - - - // Convert merged BAM to CRAM and calculate indices and statistics - SAMTOOLS_SORMADUP.out.bam - | map { meta, bam -> [ meta, bam, [] ] } - | set { ch_stat } - - - emit: - bam = ch_stat // channel: [ val(meta), /path/to/bam ] - versions = ch_versions // channel: [ versions.yml ] -} From 3d71db723a6100ee50b9e137b6e4901a01e1187f Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Fri, 2 Feb 2024 16:33:27 +0000 Subject: [PATCH 010/111] Updated the minimap2/align module to the latest nf-core version Now accepts a meta2 for the reference genome --- conf/base.config | 2 +- conf/modules.config | 8 +- modules.json | 2 +- .../nf-core/minimap2/align/environment.yml | 9 ++ modules/nf-core/minimap2/align/main.nf | 13 +- modules/nf-core/minimap2/align/meta.yml | 10 ++ .../nf-core/minimap2/align/tests/main.nf.test | 145 ++++++++++++++++++ .../minimap2/align/tests/main.nf.test.snap | 38 +++++ modules/nf-core/minimap2/align/tests/tags.yml | 2 + subworkflows/local/align_ont.nf | 9 +- subworkflows/local/align_pacbio.nf | 9 +- 11 files changed, 220 insertions(+), 27 deletions(-) create mode 100644 modules/nf-core/minimap2/align/environment.yml create mode 100644 modules/nf-core/minimap2/align/tests/main.nf.test create mode 100644 modules/nf-core/minimap2/align/tests/main.nf.test.snap create mode 100644 modules/nf-core/minimap2/align/tests/tags.yml diff --git a/conf/base.config b/conf/base.config index aa296e0..ca61f16 100644 --- a/conf/base.config +++ b/conf/base.config @@ -77,7 +77,7 @@ process { withName: MINIMAP2_ALIGN { cpus = { log_increase_cpus(4, 2*task.attempt, meta.read_count/1000000, 2) } - memory = { check_max( 14.GB * Math.ceil( Math.pow(reference.size() / 1000000000, 0.6)) * task.attempt, 'memory' ) } + memory = { check_max( 800.MB * log_increase_cpus(4, 2*task.attempt, meta.read_count/1000000, 2) + 14.GB * Math.ceil( Math.pow(meta2.genome_size / 1000000000, 0.6)) * task.attempt, 'memory' ) } time = { check_max( 3.h * Math.ceil( meta.read_count / 1000000 ) * task.attempt, 'time' ) } } diff --git a/conf/modules.config b/conf/modules.config index 6259289..d95ed06 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -45,18 +45,16 @@ process { // NOTE: minimap2 uses the decimal system ! 1G = 1,000,000,000 bp // NOTE: Math.ceil returns a double, but fortunately minimap2 accepts floating point values. // NOTE: minimap2 2.25 raises the default to 8G, which means higher memory savings on smaller genomes - // NOTE: Use `reference.size()` for now, and switch to `meta2.genome_size` once we update the modules. - // ext.args = { "-ax map-hifi --cs=short -R ${meta.read_group} -I" + Math.ceil(meta.genome_size/1e9) + 'G' } withName: '.*:.*:ALIGN_HIFI:MINIMAP2_ALIGN' { - ext.args = { "-ax map-hifi --cs=short -R ${meta.read_group} -I" + Math.ceil(reference.size()/1e9) + 'G' } + ext.args = { "-ax map-hifi --cs=short -R ${meta.read_group} -I" + Math.ceil(meta2.genome_size/1e9) + 'G' } } withName: '.*:.*:ALIGN_CLR:MINIMAP2_ALIGN' { - ext.args = { "-ax map-pb -R ${meta.read_group} -I" + Math.ceil(reference.size()/1e9) + 'G' } + ext.args = { "-ax map-pb -R ${meta.read_group} -I" + Math.ceil(meta2.genome_size/1e9) + 'G' } } withName: '.*:.*:ALIGN_ONT:MINIMAP2_ALIGN' { - ext.args = { "-ax map-ont -R ${meta.read_group} -I" + Math.ceil(reference.size()/1e9) + 'G' } + ext.args = { "-ax map-ont -R ${meta.read_group} -I" + Math.ceil(meta2.genome_size/1e9) + 'G' } } withName: '.*:CONVERT_STATS:SAMTOOLS_VIEW' { diff --git a/modules.json b/modules.json index b61ea34..9321f7d 100644 --- a/modules.json +++ b/modules.json @@ -38,7 +38,7 @@ }, "minimap2/align": { "branch": "master", - "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", + "git_sha": "efbf86bb487f288ac30660282709d9620dd6048e", "installed_by": ["modules"] }, "samtools/faidx": { diff --git a/modules/nf-core/minimap2/align/environment.yml b/modules/nf-core/minimap2/align/environment.yml new file mode 100644 index 0000000..cf6e775 --- /dev/null +++ b/modules/nf-core/minimap2/align/environment.yml @@ -0,0 +1,9 @@ +name: minimap2_align +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::minimap2=2.24 + - bioconda::samtools=1.18 + - bioconda::htslib=1.18 diff --git a/modules/nf-core/minimap2/align/main.nf b/modules/nf-core/minimap2/align/main.nf index 4da47c1..07a3215 100644 --- a/modules/nf-core/minimap2/align/main.nf +++ b/modules/nf-core/minimap2/align/main.nf @@ -3,14 +3,14 @@ process MINIMAP2_ALIGN { label 'process_medium' // Note: the versions here need to match the versions used in the mulled container below and minimap2/index - conda "bioconda::minimap2=2.24 bioconda::samtools=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' : - 'biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' }" + 'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:365b17b986c1a60c1b82c6066a9345f38317b763-0' : + 'biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:365b17b986c1a60c1b82c6066a9345f38317b763-0' }" input: tuple val(meta), path(reads) - path reference + tuple val(meta2), path(reference) val bam_format val cigar_paf_format val cigar_bam @@ -24,9 +24,10 @@ process MINIMAP2_ALIGN { task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def bam_output = bam_format ? "-a | samtools sort | samtools view -@ ${task.cpus} -b -h -o ${prefix}.bam" : "-o ${prefix}.paf" + def bam_output = bam_format ? "-a | samtools sort -@ ${task.cpus} -o ${prefix}.bam ${args2}" : "-o ${prefix}.paf" def cigar_paf = cigar_paf_format && !bam_format ? "-c" : '' def set_cigar_bam = cigar_bam && bam_format ? "-L" : '' """ diff --git a/modules/nf-core/minimap2/align/meta.yml b/modules/nf-core/minimap2/align/meta.yml index 991b39a..408522d 100644 --- a/modules/nf-core/minimap2/align/meta.yml +++ b/modules/nf-core/minimap2/align/meta.yml @@ -25,6 +25,11 @@ input: description: | List of input FASTA or FASTQ files of size 1 and 2 for single-end and paired-end data, respectively. + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test_ref'] - reference: type: file description: | @@ -63,3 +68,8 @@ authors: - "@sofstam" - "@sateeshperi" - "@jfy133" +maintainers: + - "@heuermh" + - "@sofstam" + - "@sateeshperi" + - "@jfy133" diff --git a/modules/nf-core/minimap2/align/tests/main.nf.test b/modules/nf-core/minimap2/align/tests/main.nf.test new file mode 100644 index 0000000..b634468 --- /dev/null +++ b/modules/nf-core/minimap2/align/tests/main.nf.test @@ -0,0 +1,145 @@ +nextflow_process { + + name "Test Process MINIMAP2_ALIGN" + script "../main.nf" + process "MINIMAP2_ALIGN" + + tag "modules" + tag "modules_nfcore" + tag "minimap2" + tag "minimap2/align" + + test("sarscov2 - fastq, fasta, true, false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = true + input[3] = false + input[4] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], fasta, true, false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = true + input[3] = false + input[4] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, [], true, false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + [] + ] + input[2] = true + input[3] = false + input[4] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, fasta, true, false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = true + input[3] = false + input[4] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/minimap2/align/tests/main.nf.test.snap b/modules/nf-core/minimap2/align/tests/main.nf.test.snap new file mode 100644 index 0000000..a39a169 --- /dev/null +++ b/modules/nf-core/minimap2/align/tests/main.nf.test.snap @@ -0,0 +1,38 @@ +{ + "sarscov2 - fastq, fasta, true, false, false": { + "content": [ + "test.bam", + [ + "versions.yml:md5,9e9eeae0002d466d580a9d6e0d003eb1" + ] + ], + "timestamp": "2023-12-04T12:07:06.01315354" + }, + "sarscov2 - fastq, fasta, true, false, false - stub": { + "content": [ + "test.bam", + [ + "versions.yml:md5,9e9eeae0002d466d580a9d6e0d003eb1" + ] + ], + "timestamp": "2023-12-04T12:07:24.487175659" + }, + "sarscov2 - [fastq1, fastq2], fasta, true, false, false": { + "content": [ + "test.bam", + [ + "versions.yml:md5,9e9eeae0002d466d580a9d6e0d003eb1" + ] + ], + "timestamp": "2023-12-04T12:07:12.50816279" + }, + "sarscov2 - fastq, [], true, false, false": { + "content": [ + "test.bam", + [ + "versions.yml:md5,9e9eeae0002d466d580a9d6e0d003eb1" + ] + ], + "timestamp": "2023-12-04T12:07:18.414974788" + } +} \ No newline at end of file diff --git a/modules/nf-core/minimap2/align/tests/tags.yml b/modules/nf-core/minimap2/align/tests/tags.yml new file mode 100644 index 0000000..39dba37 --- /dev/null +++ b/modules/nf-core/minimap2/align/tests/tags.yml @@ -0,0 +1,2 @@ +minimap2/align: + - "modules/nf-core/minimap2/align/**" diff --git a/subworkflows/local/align_ont.nf b/subworkflows/local/align_ont.nf index 91ed3c7..f1013d4 100644 --- a/subworkflows/local/align_ont.nf +++ b/subworkflows/local/align_ont.nf @@ -16,13 +16,8 @@ workflow ALIGN_ONT { ch_versions = Channel.empty() - // Align Fastq to Genome - fasta - | map { meta, file -> file } - | set { ch_fasta } - - // Align with minimap2. bam_format is set to true, making the output a *sorted* BAM - MINIMAP2_ALIGN ( reads, ch_fasta, true, false, false ) + // Align Fastq to Genome with minimap2. bam_format is set to true, making the output a *sorted* BAM + MINIMAP2_ALIGN ( reads, fasta, true, false, false ) ch_versions = ch_versions.mix ( MINIMAP2_ALIGN.out.versions.first() ) diff --git a/subworkflows/local/align_pacbio.nf b/subworkflows/local/align_pacbio.nf index a29e827..07855a7 100644 --- a/subworkflows/local/align_pacbio.nf +++ b/subworkflows/local/align_pacbio.nf @@ -23,13 +23,8 @@ workflow ALIGN_PACBIO { ch_versions = ch_versions.mix ( FILTER_PACBIO.out.versions ) - // Align Fastq to Genome - fasta - | map { meta, file -> file } - | set { ch_fasta } - - // Align with minimap2. bam_format is set to true, making the output a *sorted* BAM - MINIMAP2_ALIGN ( FILTER_PACBIO.out.fastq, ch_fasta, true, false, false ) + // Align Fastq to Genome with minimap2. bam_format is set to true, making the output a *sorted* BAM + MINIMAP2_ALIGN ( FILTER_PACBIO.out.fastq, fasta, true, false, false ) ch_versions = ch_versions.mix ( MINIMAP2_ALIGN.out.versions.first() ) From 7a9671c7654ae7ecbfb88616e7951afdaa7c88a7 Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Tue, 6 Feb 2024 18:31:25 +0000 Subject: [PATCH 011/111] Updated the crumble module to keep it closer to the original --- modules/nf-core/crumble/crumble.diff | 8 +------- modules/nf-core/crumble/main.nf | 2 +- 2 files changed, 2 insertions(+), 8 deletions(-) diff --git a/modules/nf-core/crumble/crumble.diff b/modules/nf-core/crumble/crumble.diff index 2c4cb1e..e5b45f9 100644 --- a/modules/nf-core/crumble/crumble.diff +++ b/modules/nf-core/crumble/crumble.diff @@ -1,13 +1,7 @@ Changes in module 'nf-core/crumble' --- modules/nf-core/crumble/main.nf +++ modules/nf-core/crumble/main.nf -@@ -30,11 +30,14 @@ - args.contains("-O cram") ? "cram" : - "sam" - def bedin = keepbed ? "-R ${keepbed}" : "" -- def bedout = bedout ? "-b ${prefix}.out.bed" : "" -+ def bedout = bedout ? "-b ${prefix}.suspicious_regions.bed" : "" - if ("$input" == "${prefix}.${extension}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" +@@ -35,6 +35,9 @@ def CRUMBLE_VERSION = '0.9.1' //WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. """ diff --git a/modules/nf-core/crumble/main.nf b/modules/nf-core/crumble/main.nf index 44c0c59..17260cb 100644 --- a/modules/nf-core/crumble/main.nf +++ b/modules/nf-core/crumble/main.nf @@ -30,7 +30,7 @@ process CRUMBLE { args.contains("-O cram") ? "cram" : "sam" def bedin = keepbed ? "-R ${keepbed}" : "" - def bedout = bedout ? "-b ${prefix}.suspicious_regions.bed" : "" + def bedout = bedout ? "-b ${prefix}.out.bed" : "" if ("$input" == "${prefix}.${extension}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" def CRUMBLE_VERSION = '0.9.1' //WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. From cfb7977af7b1750e26c8c87b012f3cb85c37828b Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Tue, 6 Feb 2024 18:37:38 +0000 Subject: [PATCH 012/111] We don't need the suspicious regions --- workflows/readmapping.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index e579d3e..af3ff3d 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -142,7 +142,7 @@ workflow READMAPPING { CONVERT_STATS.out.cram | filter { meta, bam -> meta.datatype == "pacbio" } | set { ch_pacbio_bams } - CRUMBLE ( ch_pacbio_bams, [], true ) + CRUMBLE ( ch_pacbio_bams, [], [] ) ch_versions = ch_versions.mix ( CRUMBLE.out.versions ) From 20536ea60c0217b18644c18a41efec9b9c830ce3 Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Tue, 6 Feb 2024 18:50:47 +0000 Subject: [PATCH 013/111] Also compress the Illumina files with crumble, and don't compress the pre-cumble BAMs --- conf/modules.config | 9 ++------- subworkflows/local/convert_stats.nf | 22 ++++++++++++++++++++-- workflows/readmapping.nf | 10 ---------- 3 files changed, 22 insertions(+), 19 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index d95ed06..c9e3628 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -76,12 +76,7 @@ process { withName: CRUMBLE { ext.prefix = { "${input.baseName}.crumble" } - ext.args = '-y pbccs -O cram' - publishDir = [ - path: { "${params.outdir}/read_mapping/pacbio" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + ext.args = { (meta.datatype == "pacbio" ? "-y pbccs " : "") + "-O bam" } } withName: SAMPLESHEET_CHECK { @@ -92,7 +87,7 @@ process { ] } - withName: '.*:CONVERT_STATS:.*' { + withName: '.*:CONVERT_STATS:SAMTOOLS_.*' { publishDir = [ path: { "${params.outdir}/read_mapping/${meta.datatype}" }, mode: params.publish_dir_mode, diff --git a/subworkflows/local/convert_stats.nf b/subworkflows/local/convert_stats.nf index 2ea16b9..b89928a 100644 --- a/subworkflows/local/convert_stats.nf +++ b/subworkflows/local/convert_stats.nf @@ -2,6 +2,7 @@ // Convert BAM to CRAM, create index and calculate statistics // +include { CRUMBLE } from '../../modules/nf-core/crumble/main' include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main' include { SAMTOOLS_STATS } from '../../modules/nf-core/samtools/stats/main' include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/samtools/flagstat/main' @@ -17,11 +18,28 @@ workflow CONVERT_STATS { main: ch_versions = Channel.empty() + // Compress the quality scores of Illumina and PacBio CCS alignments + bam + | branch { + meta, bam, bai -> + run_crumble : meta.datatype == "hic" || meta.datatype == "illumina" || meta.datatype == "pacbio" + [meta, bam] + no_crumble: true + } + | set { ch_bams } + + CRUMBLE ( ch_bams.run_crumble, [], [] ) + ch_versions = ch_versions.mix ( CRUMBLE.out.versions ) + // Convert BAM to CRAM - SAMTOOLS_VIEW ( bam, fasta, [] ) - ch_versions = ch_versions.mix ( SAMTOOLS_VIEW.out.versions.first() ) + CRUMBLE.out.bam + | map { meta, bam -> [meta, bam, []] } + | mix ( ch_bams.no_crumble ) + | set { ch_bams_for_conversion } + SAMTOOLS_VIEW ( ch_bams_for_conversion, fasta, [] ) + ch_versions = ch_versions.mix ( SAMTOOLS_VIEW.out.versions.first() ) // Combine CRAM and CRAI into one channel SAMTOOLS_VIEW.out.cram diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index af3ff3d..18ebd36 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -46,7 +46,6 @@ include { CONVERT_STATS } from '../subworkflows/local/convert_st // include { UNTAR } from '../modules/nf-core/untar/main' -include { CRUMBLE } from '../modules/nf-core/crumble/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' @@ -136,15 +135,6 @@ workflow READMAPPING { CONVERT_STATS ( ch_aligned_bams, PREPARE_GENOME.out.fasta ) ch_versions = ch_versions.mix ( CONVERT_STATS.out.versions ) - // - // MODULE: To compress PacBio HiFi aligned CRAM files - // - CONVERT_STATS.out.cram - | filter { meta, bam -> meta.datatype == "pacbio" } - | set { ch_pacbio_bams } - CRUMBLE ( ch_pacbio_bams, [], [] ) - ch_versions = ch_versions.mix ( CRUMBLE.out.versions ) - // // MODULE: Combine different versions.yml From 7cbb4161de0b95bd5edf271c5fadf6a7805a2119 Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Tue, 6 Feb 2024 19:14:43 +0000 Subject: [PATCH 014/111] Not needed because crumble now outputs BAM --- modules.json | 3 +-- modules/nf-core/crumble/crumble.diff | 15 --------------- modules/nf-core/crumble/main.nf | 3 --- 3 files changed, 1 insertion(+), 20 deletions(-) delete mode 100644 modules/nf-core/crumble/crumble.diff diff --git a/modules.json b/modules.json index 9321f7d..5e4c2e6 100644 --- a/modules.json +++ b/modules.json @@ -23,8 +23,7 @@ "crumble": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"], - "patch": "modules/nf-core/crumble/crumble.diff" + "installed_by": ["modules"] }, "custom/dumpsoftwareversions": { "branch": "master", diff --git a/modules/nf-core/crumble/crumble.diff b/modules/nf-core/crumble/crumble.diff deleted file mode 100644 index e5b45f9..0000000 --- a/modules/nf-core/crumble/crumble.diff +++ /dev/null @@ -1,15 +0,0 @@ -Changes in module 'nf-core/crumble' ---- modules/nf-core/crumble/main.nf -+++ modules/nf-core/crumble/main.nf -@@ -35,6 +35,9 @@ - - def CRUMBLE_VERSION = '0.9.1' //WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. - """ -+ # Need to fake REF_PATH to force crumble to use the Fasta file defined in -+ # the UR field of the @SQ headers. (bug reported to the samtools team). -+ env REF_PATH=/missing \\ - crumble \\ - $args \\ - $bedin \\ - -************************************************************ diff --git a/modules/nf-core/crumble/main.nf b/modules/nf-core/crumble/main.nf index 17260cb..2699257 100644 --- a/modules/nf-core/crumble/main.nf +++ b/modules/nf-core/crumble/main.nf @@ -35,9 +35,6 @@ process CRUMBLE { def CRUMBLE_VERSION = '0.9.1' //WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. """ - # Need to fake REF_PATH to force crumble to use the Fasta file defined in - # the UR field of the @SQ headers. (bug reported to the samtools team). - env REF_PATH=/missing \\ crumble \\ $args \\ $bedin \\ From 7ba557caa81d3ee5bd3c3e2f9729149c64d193cb Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Wed, 5 Jun 2024 18:35:55 +0000 Subject: [PATCH 015/111] Memory settings for ALIGN_ONT:MINIMAP2_ALIGN --- conf/base.config | 9 ++++++++- 1 file changed, 8 insertions(+), 1 deletion(-) diff --git a/conf/base.config b/conf/base.config index ca61f16..ca753f3 100644 --- a/conf/base.config +++ b/conf/base.config @@ -75,12 +75,19 @@ process { memory = { check_max( 8.GB + 6.GB * Math.ceil(meta2.genome_size / 1000000000) + 1600.MB * task.attempt * log_increase_cpus(6, 6*task.attempt, meta.read_count/1000000000, 2), 'memory' ) } } - withName: MINIMAP2_ALIGN { + withName: '.*:ALIGN_HIFI:MINIMAP2_ALIGN' { cpus = { log_increase_cpus(4, 2*task.attempt, meta.read_count/1000000, 2) } memory = { check_max( 800.MB * log_increase_cpus(4, 2*task.attempt, meta.read_count/1000000, 2) + 14.GB * Math.ceil( Math.pow(meta2.genome_size / 1000000000, 0.6)) * task.attempt, 'memory' ) } time = { check_max( 3.h * Math.ceil( meta.read_count / 1000000 ) * task.attempt, 'time' ) } } + // Extrapolated from the HIFI settings on the basis of 1 ONT alignment. CLR assumed to behave the same way as ONT + withName: '.*:ALIGN_(CLR|ONT):MINIMAP2_ALIGN' { + cpus = { log_increase_cpus(4, 2*task.attempt, meta.read_count/1000000, 2) } + memory = { check_max( 800.MB * log_increase_cpus(4, 2*task.attempt, meta.read_count/1000000, 2) + 30.GB * Math.ceil( Math.pow(meta2.genome_size / 1000000000, 0.6)) * task.attempt, 'memory' ) } + time = { check_max( 1.h * Math.ceil( meta.read_count / 1000000 ) * task.attempt, 'time' ) } + } + withName: CRUMBLE { // No correlation between memory usage and the number of reads or the genome size. // Most genomes seem happy with 1 GB, then some with 2 GB, then some with 5 GB. From 92b4744e03349d649c6510e0942a8c10859b106d Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Thu, 6 Jun 2024 14:35:24 +0000 Subject: [PATCH 016/111] Bumped the version and updated the changelog --- CHANGELOG.md | 19 ++++++++++++++++++- nextflow.config | 2 +- 2 files changed, 19 insertions(+), 2 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 8eb80be..8150062 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,24 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## [[1.2.2](https://github.com/sanger-tol/readmapping/releases/tag/1.2.2)] - Norwegian Ridgeback (patch 2) -[2024-05-23] +## [[1.3.0](https://github.com/sanger-tol/readmapping/releases/tag/1.3.0)] - Antipodean Opaleye - [2024-06-XX] + +### Enhancements & fixes + +- Combined steps to improve the efficiency of the pipeline, especially on large genomes +- "crumble" is now run on _every_ data type, not just PacBio + +### Software dependencies + +Note, since the pipeline is using Nextflow DSL2, each process will be run with its own [Biocontainer](https://biocontainers.pro/#/registry). This means that on occasion it is entirely possible for the pipeline to be using different versions of the same tool. However, the overall software dependency changes compared to the last release have been listed below for reference. + +| Dependency | Old version | New version | +| ---------- | ------------- | ------------- | +| `samtools` | 1.14 and 1.17 | 1.17 and 1.18 | + +> **NB:** Dependency has been **updated** if both old and new version information is present.
**NB:** Dependency has been **added** if just the new version information is present.
**NB:** Dependency has been **removed** if version information isn't present. + +## [[1.2.2](https://github.com/sanger-tol/readmapping/releases/tag/1.2.2)] - Norwegian Ridgeback (patch 2) - [2024-05-23] ### Enhancements & fixes diff --git a/nextflow.config b/nextflow.config index cd9aae7..d09f872 100644 --- a/nextflow.config +++ b/nextflow.config @@ -183,7 +183,7 @@ manifest { description = 'Pipeline to map reads generated using different sequencing technologies against a genome assembly.' mainScript = 'main.nf' nextflowVersion = '!>=22.10.1' - version = '1.2.2' + version = '1.3.0' doi = '10.5281/zenodo.6563577' } From 82996949a154e0315e14efdd8ee5c901058fa94a Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Thu, 6 Jun 2024 15:51:07 +0100 Subject: [PATCH 017/111] The patch was missing --- modules.json | 3 ++- .../samtools/merge/samtools-merge.diff | 26 +++++++++++++++++++ 2 files changed, 28 insertions(+), 1 deletion(-) create mode 100644 modules/nf-core/samtools/merge/samtools-merge.diff diff --git a/modules.json b/modules.json index 5e4c2e6..f7da822 100644 --- a/modules.json +++ b/modules.json @@ -63,7 +63,8 @@ "samtools/merge": { "branch": "master", "git_sha": "0460d316170f75f323111b4a2c0a2989f0c32013", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/samtools/merge/samtools-merge.diff" }, "samtools/stats": { "branch": "master", diff --git a/modules/nf-core/samtools/merge/samtools-merge.diff b/modules/nf-core/samtools/merge/samtools-merge.diff new file mode 100644 index 0000000..cca0b3c --- /dev/null +++ b/modules/nf-core/samtools/merge/samtools-merge.diff @@ -0,0 +1,26 @@ +Changes in module 'nf-core/samtools/merge' +--- modules/nf-core/samtools/merge/main.nf ++++ modules/nf-core/samtools/merge/main.nf +@@ -26,6 +26,11 @@ + def args = task.ext.args ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + def file_type = input_files instanceof List ? input_files[0].getExtension() : input_files.getExtension() ++ if (input_files instanceof List) { ++ sorted_input_files = input_files.toSorted({it.name}).join(' ') ++ } else { ++ sorted_input_files = input_files ++ } + def reference = fasta ? "--reference ${fasta}" : "" + """ + samtools \\ +@@ -34,7 +39,7 @@ + $args \\ + ${reference} \\ + ${prefix}.${file_type} \\ +- $input_files ++ $sorted_input_files + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + +************************************************************ From bb7e6be6f978f60fcb39b55f11bfbe0bb9ea70a8 Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Sat, 22 Jun 2024 22:15:13 +0100 Subject: [PATCH 018/111] The patch is not needed anymore since I have combined this module with SAMTOOLS_FASTQ into SAMTOOLS_FILTERTOFASTQ --- modules.json | 3 +- modules/nf-core/samtools/view/main.nf | 17 +++++---- .../nf-core/samtools/view/samtools-view.diff | 36 ------------------- 3 files changed, 9 insertions(+), 47 deletions(-) delete mode 100644 modules/nf-core/samtools/view/samtools-view.diff diff --git a/modules.json b/modules.json index f7da822..81077ac 100644 --- a/modules.json +++ b/modules.json @@ -74,8 +74,7 @@ "samtools/view": { "branch": "master", "git_sha": "3ffae3598260a99e8db3207dead9f73f87f90d1f", - "installed_by": ["modules"], - "patch": "modules/nf-core/samtools/view/samtools-view.diff" + "installed_by": ["modules"] }, "untar": { "branch": "master", diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf index aea5a42..cb91fac 100644 --- a/modules/nf-core/samtools/view/main.nf +++ b/modules/nf-core/samtools/view/main.nf @@ -13,14 +13,13 @@ process SAMTOOLS_VIEW { path qname output: - tuple val(meta), path("*.bam"), emit: bam, optional: true - tuple val(meta), path("*.cram"), emit: cram, optional: true - tuple val(meta), path("*.sam"), emit: sam, optional: true - tuple val(meta), path("*.bai"), emit: bai, optional: true - tuple val(meta), path("*.csi"), emit: csi, optional: true - tuple val(meta), path("*.crai"), emit: crai, optional: true - tuple val(meta), path("*.unoutput"), emit: unoutput, optional: true - path "versions.yml", emit: versions + tuple val(meta), path("*.bam"), emit: bam, optional: true + tuple val(meta), path("*.cram"), emit: cram, optional: true + tuple val(meta), path("*.sam"), emit: sam, optional: true + tuple val(meta), path("*.bai"), emit: bai, optional: true + tuple val(meta), path("*.csi"), emit: csi, optional: true + tuple val(meta), path("*.crai"), emit: crai, optional: true + path "versions.yml", emit: versions when: task.ext.when == null || task.ext.when @@ -30,7 +29,7 @@ process SAMTOOLS_VIEW { def args2 = task.ext.args2 ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def reference = fasta ? "--reference ${fasta}" : "" - def readnames = qname ? "--qname-file ${qname} --unoutput ${prefix}.unoutput": "" + def readnames = qname ? "--qname-file ${qname}": "" def file_type = args.contains("--output-fmt sam") ? "sam" : args.contains("--output-fmt bam") ? "bam" : args.contains("--output-fmt cram") ? "cram" : diff --git a/modules/nf-core/samtools/view/samtools-view.diff b/modules/nf-core/samtools/view/samtools-view.diff deleted file mode 100644 index 49c9241..0000000 --- a/modules/nf-core/samtools/view/samtools-view.diff +++ /dev/null @@ -1,36 +0,0 @@ -Changes in module 'nf-core/samtools/view' ---- modules/nf-core/samtools/view/main.nf -+++ modules/nf-core/samtools/view/main.nf -@@ -13,13 +13,14 @@ - path qname - - output: -- tuple val(meta), path("*.bam"), emit: bam, optional: true -- tuple val(meta), path("*.cram"), emit: cram, optional: true -- tuple val(meta), path("*.sam"), emit: sam, optional: true -- tuple val(meta), path("*.bai"), emit: bai, optional: true -- tuple val(meta), path("*.csi"), emit: csi, optional: true -- tuple val(meta), path("*.crai"), emit: crai, optional: true -- path "versions.yml", emit: versions -+ tuple val(meta), path("*.bam"), emit: bam, optional: true -+ tuple val(meta), path("*.cram"), emit: cram, optional: true -+ tuple val(meta), path("*.sam"), emit: sam, optional: true -+ tuple val(meta), path("*.bai"), emit: bai, optional: true -+ tuple val(meta), path("*.csi"), emit: csi, optional: true -+ tuple val(meta), path("*.crai"), emit: crai, optional: true -+ tuple val(meta), path("*.unoutput"), emit: unoutput, optional: true -+ path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when -@@ -29,7 +30,7 @@ - def args2 = task.ext.args2 ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def reference = fasta ? "--reference ${fasta}" : "" -- def readnames = qname ? "--qname-file ${qname}": "" -+ def readnames = qname ? "--qname-file ${qname} --unoutput ${prefix}.unoutput": "" - def file_type = args.contains("--output-fmt sam") ? "sam" : - args.contains("--output-fmt bam") ? "bam" : - args.contains("--output-fmt cram") ? "cram" : - -************************************************************ From 18cf83640b0f2ec890bcacabbb8d9ccefa54dad0 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 26 Jun 2024 11:08:07 +0100 Subject: [PATCH 019/111] add --header optional argument --- nextflow.config | 1 + nextflow_schema.json | 9 ++++++++- 2 files changed, 9 insertions(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index d09f872..b10fe94 100644 --- a/nextflow.config +++ b/nextflow.config @@ -14,6 +14,7 @@ params { vector_db = "${projectDir}/assets/vectorDB.tar.gz" bwamem2_index = null fasta = null + header = null // Execution options use_work_dir_as_temp = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 737b3c2..20533b4 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -26,7 +26,8 @@ "type": "string", "format": "directory-path", "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", - "fa_icon": "fas fa-folder-open" + "fa_icon": "fas fa-folder-open", + "default": "./results" }, "vector_db": { "type": "string", @@ -64,6 +65,12 @@ "description": "Path to directory or tar.gz archive for pre-built BWAMEM2 index.", "format": "path", "fa_icon": "fas fa-bezier-curve" + }, + "header": { + "type": "string", + "format": "path", + "description": "Optional template header file for BAM/CRAM outputs", + "fa_icon": "far fa-file-code" } } }, From 7a11e6be0b1ac76d9eaa05abb4ff68f5939ebada Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 26 Jun 2024 11:08:50 +0100 Subject: [PATCH 020/111] install nf-core/samtools/reheader module --- .../nf-core/samtools/reheader/environment.yml | 8 +++ modules/nf-core/samtools/reheader/main.nf | 49 ++++++++++++++++++ modules/nf-core/samtools/reheader/meta.yml | 50 +++++++++++++++++++ 3 files changed, 107 insertions(+) create mode 100644 modules/nf-core/samtools/reheader/environment.yml create mode 100644 modules/nf-core/samtools/reheader/main.nf create mode 100644 modules/nf-core/samtools/reheader/meta.yml diff --git a/modules/nf-core/samtools/reheader/environment.yml b/modules/nf-core/samtools/reheader/environment.yml new file mode 100644 index 0000000..aa9c798 --- /dev/null +++ b/modules/nf-core/samtools/reheader/environment.yml @@ -0,0 +1,8 @@ +name: samtools_reheader +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.20 + - bioconda::htslib=1.20 diff --git a/modules/nf-core/samtools/reheader/main.nf b/modules/nf-core/samtools/reheader/main.nf new file mode 100644 index 0000000..24192ea --- /dev/null +++ b/modules/nf-core/samtools/reheader/main.nf @@ -0,0 +1,49 @@ +process SAMTOOLS_REHEADER { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0': + 'biocontainers/samtools:1.20--h50ea8bc_0' }" + + input: + tuple val(meta), path(bam) + + output: + tuple val(meta), path("*.bam"), emit: bam + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + if ("$bam" == "${meta.id}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + samtools \\ + reheader \\ + $args \\ + $bam \\ + > ${prefix}.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bam + + ccat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/samtools/reheader/meta.yml b/modules/nf-core/samtools/reheader/meta.yml new file mode 100644 index 0000000..a5e1591 --- /dev/null +++ b/modules/nf-core/samtools/reheader/meta.yml @@ -0,0 +1,50 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json + +name: "samtools_reheader" +description: | + Replace the header in the bam file with the header generated by the command. + This command is much faster than replacing the header with a BAM→SAM→BAM conversion. +keywords: + - reheader + - cram + - bam + - genomics +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: http://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - bam: + type: file + description: BAM/CRAM file to be reheaded + pattern: "*.{bam,cram}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - bam: + type: file + description: Reheaded BAM/CRAM file + pattern: "*.{bam,cram}" +authors: + - "@louislenezet" +maintainers: + - "@louislenezet" From 6fcc80a093beed655ef8b0dd76706f2c0dde627c Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 26 Jun 2024 11:09:21 +0100 Subject: [PATCH 021/111] install nf-core/samtools/reheader module --- modules.json | 5 +++++ 1 file changed, 5 insertions(+) diff --git a/modules.json b/modules.json index 81077ac..3331b0d 100644 --- a/modules.json +++ b/modules.json @@ -66,6 +66,11 @@ "installed_by": ["modules"], "patch": "modules/nf-core/samtools/merge/samtools-merge.diff" }, + "samtools/reheader": { + "branch": "master", + "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", + "installed_by": ["modules"] + }, "samtools/stats": { "branch": "master", "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", From 55172c730bc9ddf4fdf6e482d9f084f18d8ae7ec Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 28 Jun 2024 13:50:28 +0100 Subject: [PATCH 022/111] default config for samtools_reheader --- conf/modules.config | 7 +++++++ 1 file changed, 7 insertions(+) diff --git a/conf/modules.config b/conf/modules.config index c9e3628..3533e40 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -28,6 +28,13 @@ process { ext.prefix = { "${meta.id}.merge" } } + // If custom header provided, this is inserted in place of existing + // @HD and @SQ lines, while preserving any other header entries + withName: SAMTOOLS_REHEADER { + ext.prefix = { "${meta.id}.reheader" } + ext.args = { '-c \'grep -v ^@HD\\|^@SQ\'' } + } + withName: SAMTOOLS_COLLATETOFASTA { ext.args = { (params.use_work_dir_as_temp ? "-T." : "") } } From 7a460b04b0e59a6c6e74b4aa00e4f6d7bf7ad2a8 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 28 Jun 2024 13:52:04 +0100 Subject: [PATCH 023/111] switch to custom samtools reheader wrapper in local module --- .../samtools_replaceheader.nf} | 34 +++++++------ .../nf-core/samtools/reheader/environment.yml | 8 --- modules/nf-core/samtools/reheader/meta.yml | 50 ------------------- 3 files changed, 18 insertions(+), 74 deletions(-) rename modules/{nf-core/samtools/reheader/main.nf => local/samtools_replaceheader.nf} (50%) delete mode 100644 modules/nf-core/samtools/reheader/environment.yml delete mode 100644 modules/nf-core/samtools/reheader/meta.yml diff --git a/modules/nf-core/samtools/reheader/main.nf b/modules/local/samtools_replaceheader.nf similarity index 50% rename from modules/nf-core/samtools/reheader/main.nf rename to modules/local/samtools_replaceheader.nf index 24192ea..6e23e46 100644 --- a/modules/nf-core/samtools/reheader/main.nf +++ b/modules/local/samtools_replaceheader.nf @@ -2,32 +2,34 @@ process SAMTOOLS_REHEADER { tag "$meta.id" label 'process_single' - conda "${moduleDir}/environment.yml" + conda "bioconda::samtools=1.17" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0': - 'biocontainers/samtools:1.20--h50ea8bc_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : + 'biocontainers/samtools:1.17--h00cdaf9_0' }" input: - tuple val(meta), path(bam) + tuple val(meta), path(file), path(header) output: - tuple val(meta), path("*.bam"), emit: bam - path "versions.yml" , emit: versions + tuple val(meta), path("*.${meta.suffix}"), emit: bam + path "versions.yml", emit: versions when: task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + def suffix = "${meta.suffix}" + def args = task.ext.args ?: '' - if ("$bam" == "${meta.id}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + if ("$file" == "${prefix}.${suffix}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" """ - samtools \\ - reheader \\ - $args \\ - $bam \\ - > ${prefix}.bam + if [ ! -z "${args}" ]; then + samtools reheader ${args} ${file} | \\ + samtools reheader ${header} - > ${prefix}.${suffix} + else + samtools reheader ${header} ${file} > ${prefix}.${suffix} + fi cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -36,12 +38,12 @@ process SAMTOOLS_REHEADER { """ stub: - def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + def suffix = file.name.split('\\.')[-1] """ - touch ${prefix}.bam + touch ${prefix}.${suffix} - ccat <<-END_VERSIONS > versions.yml + cat <<-END_VERSIONS > versions.yml "${task.process}": samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS diff --git a/modules/nf-core/samtools/reheader/environment.yml b/modules/nf-core/samtools/reheader/environment.yml deleted file mode 100644 index aa9c798..0000000 --- a/modules/nf-core/samtools/reheader/environment.yml +++ /dev/null @@ -1,8 +0,0 @@ -name: samtools_reheader -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::samtools=1.20 - - bioconda::htslib=1.20 diff --git a/modules/nf-core/samtools/reheader/meta.yml b/modules/nf-core/samtools/reheader/meta.yml deleted file mode 100644 index a5e1591..0000000 --- a/modules/nf-core/samtools/reheader/meta.yml +++ /dev/null @@ -1,50 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json - -name: "samtools_reheader" -description: | - Replace the header in the bam file with the header generated by the command. - This command is much faster than replacing the header with a BAM→SAM→BAM conversion. -keywords: - - reheader - - cram - - bam - - genomics -tools: - - samtools: - description: | - SAMtools is a set of utilities for interacting with and post-processing - short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. - These files are generated as output by short read aligners like BWA. - homepage: http://www.htslib.org/ - documentation: http://www.htslib.org/doc/samtools.html - doi: 10.1093/bioinformatics/btp352 - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test', single_end:false ]` - - bam: - type: file - description: BAM/CRAM file to be reheaded - pattern: "*.{bam,cram}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test', single_end:false ]` - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - - bam: - type: file - description: Reheaded BAM/CRAM file - pattern: "*.{bam,cram}" -authors: - - "@louislenezet" -maintainers: - - "@louislenezet" From eeb3b2980eb4ccd987f95aea4d30628de3d398be Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 28 Jun 2024 13:52:19 +0100 Subject: [PATCH 024/111] switch to custom samtools reheader wrapper in local module --- modules.json | 67 ++++++++++++++++++++++++++++++++++++---------------- 1 file changed, 46 insertions(+), 21 deletions(-) diff --git a/modules.json b/modules.json index 3331b0d..bc96f67 100644 --- a/modules.json +++ b/modules.json @@ -8,86 +8,111 @@ "blast/blastn": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwamem2/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwamem2/mem": { "branch": "master", "git_sha": "0460d316170f75f323111b4a2c0a2989f0c32013", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "crumble": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "gunzip": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "minimap2/align": { "branch": "master", "git_sha": "efbf86bb487f288ac30660282709d9620dd6048e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/faidx": { "branch": "master", "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/fastq": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/flagstat": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/idxstats": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/merge": { "branch": "master", "git_sha": "0460d316170f75f323111b4a2c0a2989f0c32013", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/samtools/merge/samtools-merge.diff" }, - "samtools/reheader": { - "branch": "master", - "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", - "installed_by": ["modules"] - }, "samtools/stats": { "branch": "master", "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/view": { "branch": "master", "git_sha": "3ffae3598260a99e8db3207dead9f73f87f90d1f", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "untar": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] } } } } } -} +} \ No newline at end of file From 0e58adef543f2066637a2c9caced65c2f6d98fb8 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 28 Jun 2024 13:53:02 +0100 Subject: [PATCH 025/111] added subworkflow to insert custom sam header before cram generation --- workflows/readmapping.nf | 32 +++++++++++++++++++++++++++++--- 1 file changed, 29 insertions(+), 3 deletions(-) diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index 18ebd36..854d3be 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -21,6 +21,13 @@ if (params.fasta) { ch_fasta = Channel.fromPath(params.fasta) } else { exit 1, ' ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ +// +// MODULE: Local modules +// + +include { SAMTOOLS_REHEADER } from '../modules/local/samtools_replaceheader' + + // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // @@ -48,7 +55,6 @@ include { CONVERT_STATS } from '../subworkflows/local/convert_st include { UNTAR } from '../modules/nf-core/untar/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ RUN MAIN WORKFLOW @@ -125,14 +131,34 @@ workflow READMAPPING { ALIGN_ONT ( PREPARE_GENOME.out.fasta, ch_reads.ont ) ch_versions = ch_versions.mix ( ALIGN_ONT.out.versions ) - + // gather alignments ch_aligned_bams = Channel.empty() | mix( ALIGN_HIC.out.bam ) | mix( ALIGN_ILLUMINA.out.bam ) | mix( ALIGN_HIFI.out.bam ) | mix( ALIGN_CLR.out.bam ) | mix( ALIGN_ONT.out.bam ) - CONVERT_STATS ( ch_aligned_bams, PREPARE_GENOME.out.fasta ) + + // Optionally insert params.header information to bams + ch_reheadered_bams = Channel.empty() + if ( params.header ) { + ch_combined = ch_aligned_bams.map { meta, bam, _ -> + def suffix = bam instanceof List ? bam[0].getExtension() : bam.getExtension() + meta.suffix = suffix // add suffix to meta so output matches input type + [meta, bam, file( params.header )] + } + SAMTOOLS_REHEADER( ch_combined ) + ch_reheadered_bams = SAMTOOLS_REHEADER.out.bam.map { bam -> bam + [[]] } + ch_versions = ch_versions.mix ( SAMTOOLS_REHEADER.out.versions ) + } else { + // If no reheadering is done, use the original aligned bams + ch_reheadered_bams = ch_aligned_bams + } + ch_aligned_bams.view() + ch_reheadered_bams.view() + + // convert to cram and gather stats + CONVERT_STATS ( ch_reheadered_bams, PREPARE_GENOME.out.fasta ) ch_versions = ch_versions.mix ( CONVERT_STATS.out.versions ) From 6e065b9688258432a2789ef8d1115936e3c96355 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 28 Jun 2024 14:01:23 +0100 Subject: [PATCH 026/111] example header template for profile/test --- assets/GCA_922984935.2.subset.header.sam | 2 ++ 1 file changed, 2 insertions(+) create mode 100644 assets/GCA_922984935.2.subset.header.sam diff --git a/assets/GCA_922984935.2.subset.header.sam b/assets/GCA_922984935.2.subset.header.sam new file mode 100644 index 0000000..ded409f --- /dev/null +++ b/assets/GCA_922984935.2.subset.header.sam @@ -0,0 +1,2 @@ +@HD VN:1.0 SO:unsorted +@SQ SN:OV277441.1 LN:7999920 M5:0457acf8690429f1c98ee545cb8573b8 UR:https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/assembly/release/mMelMel3.1_paternal_haplotype/GCA_922984935.2.subset.fasta.gz AS:GCA_922984935.2 AN:SUPER_1 SP:Meles meles From 23bcb91a16e0bb177bde5dd5fe5e5c72f9276380 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 28 Jun 2024 14:08:32 +0100 Subject: [PATCH 027/111] remove test prints --- workflows/readmapping.nf | 2 -- 1 file changed, 2 deletions(-) diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index 854d3be..6fbc2ee 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -154,8 +154,6 @@ workflow READMAPPING { // If no reheadering is done, use the original aligned bams ch_reheadered_bams = ch_aligned_bams } - ch_aligned_bams.view() - ch_reheadered_bams.view() // convert to cram and gather stats CONVERT_STATS ( ch_reheadered_bams, PREPARE_GENOME.out.fasta ) From 79aef7df0c9b9b051b801a457b569f199233eeba Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 28 Jun 2024 14:10:00 +0100 Subject: [PATCH 028/111] remove test prints and minor cleanup --- workflows/readmapping.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index 6fbc2ee..1395230 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -148,7 +148,7 @@ workflow READMAPPING { [meta, bam, file( params.header )] } SAMTOOLS_REHEADER( ch_combined ) - ch_reheadered_bams = SAMTOOLS_REHEADER.out.bam.map { bam -> bam + [[]] } + ch_reheadered_bams = SAMTOOLS_REHEADER.out.bam.map { bam -> bam + [] } ch_versions = ch_versions.mix ( SAMTOOLS_REHEADER.out.versions ) } else { // If no reheadering is done, use the original aligned bams From 36079fe60656c7577dc1bf2df9f2b447524a890f Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 28 Jun 2024 14:17:50 +0100 Subject: [PATCH 029/111] remove test prints and minor cleanup --- workflows/readmapping.nf | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index 1395230..844d2dc 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -148,10 +148,9 @@ workflow READMAPPING { [meta, bam, file( params.header )] } SAMTOOLS_REHEADER( ch_combined ) - ch_reheadered_bams = SAMTOOLS_REHEADER.out.bam.map { bam -> bam + [] } + ch_reheadered_bams = SAMTOOLS_REHEADER.out.bam.map { bam -> bam + [[]] } ch_versions = ch_versions.mix ( SAMTOOLS_REHEADER.out.versions ) } else { - // If no reheadering is done, use the original aligned bams ch_reheadered_bams = ch_aligned_bams } From c6568fe18cc3d763b6183632861b93b9c2aec6a6 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 28 Jun 2024 14:43:29 +0100 Subject: [PATCH 030/111] docs for samtools_reheader --- docs/output.md | 4 ++++ docs/usage.md | 3 +++ 2 files changed, 7 insertions(+) diff --git a/docs/output.md b/docs/output.md index 9722d11..836af2f 100644 --- a/docs/output.md +++ b/docs/output.md @@ -79,6 +79,10 @@ The filtered PacBio reads are aligned with `MINIMAP2_ALIGN`. The sorted and merg ## Alignment post-processing +### External metadata + +If provided using the `--header` option, all output alignments (`*.cram`) will include any additional metadata supplied as a SAM header template, replacing the existing *@HD* and *@SD* entries (note that this behaviour can be altered by modifying the `ext.args` for `SAMTOOLS_REHEADER` in `modules.config`. + ### Statistics The output alignments, along with the index, are used to calculate mapping statistics. Output files are generated using `SAMTOOLS_STATS`, `SAMTOOLS_FLAGSTAT` and `SAMTOOLS_IDXSTATS`. diff --git a/docs/usage.md b/docs/usage.md index 8c6923d..d63cf0f 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -66,6 +66,9 @@ work # Directory containing the nextflow working files # Other nextflow hidden files, eg. history of pipeline runs and old logs. ``` +You can also optionally supply a template SAM header using the `--header` option to add or modify metadata associated with the assembly, which will be incorporated into the output alignments. + + ### Updating the pipeline When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: From af7636edfc7df93636f7b741216e7627b7e75d4c Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 28 Jun 2024 16:04:20 +0100 Subject: [PATCH 031/111] prettier linting --- docs/output.md | 2 +- docs/usage.md | 1 - modules.json | 62 +++++++++++++------------------------------------- 3 files changed, 17 insertions(+), 48 deletions(-) diff --git a/docs/output.md b/docs/output.md index 836af2f..e9b8b8c 100644 --- a/docs/output.md +++ b/docs/output.md @@ -81,7 +81,7 @@ The filtered PacBio reads are aligned with `MINIMAP2_ALIGN`. The sorted and merg ### External metadata -If provided using the `--header` option, all output alignments (`*.cram`) will include any additional metadata supplied as a SAM header template, replacing the existing *@HD* and *@SD* entries (note that this behaviour can be altered by modifying the `ext.args` for `SAMTOOLS_REHEADER` in `modules.config`. +If provided using the `--header` option, all output alignments (`*.cram`) will include any additional metadata supplied as a SAM header template, replacing the existing _@HD_ and _@SD_ entries (note that this behaviour can be altered by modifying the `ext.args` for `SAMTOOLS_REHEADER` in `modules.config`. ### Statistics diff --git a/docs/usage.md b/docs/usage.md index d63cf0f..f539695 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -68,7 +68,6 @@ work # Directory containing the nextflow working files You can also optionally supply a template SAM header using the `--header` option to add or modify metadata associated with the assembly, which will be incorporated into the output alignments. - ### Updating the pipeline When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: diff --git a/modules.json b/modules.json index bc96f67..81077ac 100644 --- a/modules.json +++ b/modules.json @@ -8,111 +8,81 @@ "blast/blastn": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwamem2/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwamem2/mem": { "branch": "master", "git_sha": "0460d316170f75f323111b4a2c0a2989f0c32013", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "crumble": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "gunzip": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "minimap2/align": { "branch": "master", "git_sha": "efbf86bb487f288ac30660282709d9620dd6048e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/faidx": { "branch": "master", "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/fastq": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/flagstat": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/idxstats": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/merge": { "branch": "master", "git_sha": "0460d316170f75f323111b4a2c0a2989f0c32013", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/samtools/merge/samtools-merge.diff" }, "samtools/stats": { "branch": "master", "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/view": { "branch": "master", "git_sha": "3ffae3598260a99e8db3207dead9f73f87f90d1f", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "untar": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } } } } -} \ No newline at end of file +} From 755731e7abc89c07413528722d818baaf59a2d8c Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 1 Jul 2024 10:03:24 +0100 Subject: [PATCH 032/111] moved assets/{example}.header.sam to hosted test_data --- assets/GCA_922984935.2.subset.header.sam | 2 -- 1 file changed, 2 deletions(-) delete mode 100644 assets/GCA_922984935.2.subset.header.sam diff --git a/assets/GCA_922984935.2.subset.header.sam b/assets/GCA_922984935.2.subset.header.sam deleted file mode 100644 index ded409f..0000000 --- a/assets/GCA_922984935.2.subset.header.sam +++ /dev/null @@ -1,2 +0,0 @@ -@HD VN:1.0 SO:unsorted -@SQ SN:OV277441.1 LN:7999920 M5:0457acf8690429f1c98ee545cb8573b8 UR:https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/assembly/release/mMelMel3.1_paternal_haplotype/GCA_922984935.2.subset.fasta.gz AS:GCA_922984935.2 AN:SUPER_1 SP:Meles meles From 1593a8dd750768cae7fb4f404b8751726576925a Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 1 Jul 2024 10:11:16 +0100 Subject: [PATCH 033/111] add reheader steps to -profile test runs --- conf/test.config | 1 + 1 file changed, 1 insertion(+) diff --git a/conf/test.config b/conf/test.config index 015885c..e269f68 100644 --- a/conf/test.config +++ b/conf/test.config @@ -24,4 +24,5 @@ params { // Fasta references fasta = "https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/assembly/release/mMelMel3.1_paternal_haplotype/GCA_922984935.2.subset.fasta.gz" + header = "https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/assembly/release/mMelMel3.1_paternal_haplotype/GCA_922984935.2.subset.header.sam" } From 4ab46b0e659dc4c407399dfb5414abbf9dedd9f9 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Thu, 4 Jul 2024 13:32:54 +0100 Subject: [PATCH 034/111] fix reheader to only replace SQ lines; maintains input order --- conf/modules.config | 1 - modules/local/samtools_replaceheader.nf | 22 +++++++++++++++------- 2 files changed, 15 insertions(+), 8 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 3533e40..1bb619f 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -32,7 +32,6 @@ process { // @HD and @SQ lines, while preserving any other header entries withName: SAMTOOLS_REHEADER { ext.prefix = { "${meta.id}.reheader" } - ext.args = { '-c \'grep -v ^@HD\\|^@SQ\'' } } withName: SAMTOOLS_COLLATETOFASTA { diff --git a/modules/local/samtools_replaceheader.nf b/modules/local/samtools_replaceheader.nf index 6e23e46..0abbffe 100644 --- a/modules/local/samtools_replaceheader.nf +++ b/modules/local/samtools_replaceheader.nf @@ -20,16 +20,24 @@ process SAMTOOLS_REHEADER { script: def prefix = task.ext.prefix ?: "${meta.id}" def suffix = "${meta.suffix}" - def args = task.ext.args ?: '' if ("$file" == "${prefix}.${suffix}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" """ - if [ ! -z "${args}" ]; then - samtools reheader ${args} ${file} | \\ - samtools reheader ${header} - > ${prefix}.${suffix} - else - samtools reheader ${header} ${file} > ${prefix}.${suffix} - fi + # Replace SQ lines with those from external template + ( samtools view --no-PG --header-only ${file} | \\ + grep -v ^@SQ && grep ^@SQ ${header} ) > .temp.header.sam + + # custom sort for readability (retain order of insertion but sort groups by tag) + ( grep ^@HD .temp.header.sam && \ + grep ^@SQ .temp.header.sam && \ + grep ^@RG .temp.header.sam && \ + grep ^@PG .temp.header.sam && \ + if grep -q -E -v '^@HD|^@SQ|^@RG|^@PG' .temp.header.sam; then \ + grep -v -E '^@HD|^@SQ|^@RG|^@PG' .temp.header.sam; \ + fi; ) > .temp.sorted.header.sam + + # Insert new header into file + samtools reheader .temp.sorted.header.sam ${file} > ${prefix}.${suffix} cat <<-END_VERSIONS > versions.yml "${task.process}": From a1725a027b06d3be424c0d15ba7f4ce9e8fb6034 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 5 Jul 2024 14:02:37 +0100 Subject: [PATCH 035/111] Update modules/local/samtools_replaceheader.nf Co-authored-by: Matthieu Muffato --- modules/local/samtools_replaceheader.nf | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/modules/local/samtools_replaceheader.nf b/modules/local/samtools_replaceheader.nf index 0abbffe..2d3496b 100644 --- a/modules/local/samtools_replaceheader.nf +++ b/modules/local/samtools_replaceheader.nf @@ -32,9 +32,8 @@ process SAMTOOLS_REHEADER { grep ^@SQ .temp.header.sam && \ grep ^@RG .temp.header.sam && \ grep ^@PG .temp.header.sam && \ - if grep -q -E -v '^@HD|^@SQ|^@RG|^@PG' .temp.header.sam; then \ - grep -v -E '^@HD|^@SQ|^@RG|^@PG' .temp.header.sam; \ - fi; ) > .temp.sorted.header.sam + grep -v -E '^@HD|^@SQ|^@RG|^@PG' .temp.header.sam || false; \ + ) > .temp.sorted.header.sam # Insert new header into file samtools reheader .temp.sorted.header.sam ${file} > ${prefix}.${suffix} From b6e64771761169efb18fcd1f0d3170483934c689 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 5 Jul 2024 17:49:24 +0100 Subject: [PATCH 036/111] dynamic output but this time it works --- modules/local/samtools_replaceheader.nf | 21 +++++++++++---------- 1 file changed, 11 insertions(+), 10 deletions(-) diff --git a/modules/local/samtools_replaceheader.nf b/modules/local/samtools_replaceheader.nf index 2d3496b..c28cfbc 100644 --- a/modules/local/samtools_replaceheader.nf +++ b/modules/local/samtools_replaceheader.nf @@ -11,15 +11,16 @@ process SAMTOOLS_REHEADER { tuple val(meta), path(file), path(header) output: - tuple val(meta), path("*.${meta.suffix}"), emit: bam + tuple val(meta), path("${prefix}.${suffix}"), optional:true, emit: bam + tuple val(meta), path("${prefix}.${suffix}"), optional:true, emit: cram path "versions.yml", emit: versions when: task.ext.when == null || task.ext.when script: - def prefix = task.ext.prefix ?: "${meta.id}" - def suffix = "${meta.suffix}" + prefix = task.ext.prefix ?: "${meta.id}" + suffix = file.getExtension() if ("$file" == "${prefix}.${suffix}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" """ @@ -28,11 +29,11 @@ process SAMTOOLS_REHEADER { grep -v ^@SQ && grep ^@SQ ${header} ) > .temp.header.sam # custom sort for readability (retain order of insertion but sort groups by tag) - ( grep ^@HD .temp.header.sam && \ - grep ^@SQ .temp.header.sam && \ - grep ^@RG .temp.header.sam && \ - grep ^@PG .temp.header.sam && \ - grep -v -E '^@HD|^@SQ|^@RG|^@PG' .temp.header.sam || false; \ + ( grep ^@HD .temp.header.sam || true && \ + grep ^@SQ .temp.header.sam || true && \ + grep ^@RG .temp.header.sam || true && \ + grep ^@PG .temp.header.sam || true && \ + grep -v -E '^@HD|^@SQ|^@RG|^@PG' .temp.header.sam || true; \ ) > .temp.sorted.header.sam # Insert new header into file @@ -45,8 +46,8 @@ process SAMTOOLS_REHEADER { """ stub: - def prefix = task.ext.prefix ?: "${meta.id}" - def suffix = file.name.split('\\.')[-1] + prefix = task.ext.prefix ?: "${meta.id}" + suffix = file.getExtension() """ touch ${prefix}.${suffix} From 8ade9cfe8c7e8309b703599437990aa2af1038cd Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 5 Jul 2024 18:47:56 +0100 Subject: [PATCH 037/111] simplify samtools_replaceheader inputs --- modules/local/samtools_replaceheader.nf | 3 ++- workflows/readmapping.nf | 11 +++-------- 2 files changed, 5 insertions(+), 9 deletions(-) diff --git a/modules/local/samtools_replaceheader.nf b/modules/local/samtools_replaceheader.nf index c28cfbc..06e45f6 100644 --- a/modules/local/samtools_replaceheader.nf +++ b/modules/local/samtools_replaceheader.nf @@ -8,7 +8,8 @@ process SAMTOOLS_REHEADER { 'biocontainers/samtools:1.17--h00cdaf9_0' }" input: - tuple val(meta), path(file), path(header) + tuple val(meta), path(file) + path(header) output: tuple val(meta), path("${prefix}.${suffix}"), optional:true, emit: bam diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index 844d2dc..f1571ad 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -13,7 +13,7 @@ for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true // Check mandatory parameters if (params.input) { ch_input = Channel.fromPath(params.input) } else { exit 1, 'Input samplesheet not specified!' } if (params.fasta) { ch_fasta = Channel.fromPath(params.fasta) } else { exit 1, 'Genome fasta file not specified!' } - +if (params.header) { ch_header = Channel.fromPath(params.header) } /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -142,13 +142,8 @@ workflow READMAPPING { // Optionally insert params.header information to bams ch_reheadered_bams = Channel.empty() if ( params.header ) { - ch_combined = ch_aligned_bams.map { meta, bam, _ -> - def suffix = bam instanceof List ? bam[0].getExtension() : bam.getExtension() - meta.suffix = suffix // add suffix to meta so output matches input type - [meta, bam, file( params.header )] - } - SAMTOOLS_REHEADER( ch_combined ) - ch_reheadered_bams = SAMTOOLS_REHEADER.out.bam.map { bam -> bam + [[]] } + SAMTOOLS_REHEADER( ch_aligned_bams, ch_header.first() ) + ch_reheadered_bams = SAMTOOLS_REHEADER.out.bam ch_versions = ch_versions.mix ( SAMTOOLS_REHEADER.out.versions ) } else { ch_reheadered_bams = ch_aligned_bams From a8abc897f926d74ee98f1b24907688c6ffc96fd2 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 5 Jul 2024 18:48:24 +0100 Subject: [PATCH 038/111] remove some of the back-and-forth with bai placeholders --- subworkflows/local/align_ont.nf | 1 - subworkflows/local/align_pacbio.nf | 1 - subworkflows/local/align_short.nf | 3 ++- subworkflows/local/convert_stats.nf | 6 ++++-- 4 files changed, 6 insertions(+), 5 deletions(-) diff --git a/subworkflows/local/align_ont.nf b/subworkflows/local/align_ont.nf index f1013d4..33d42b5 100644 --- a/subworkflows/local/align_ont.nf +++ b/subworkflows/local/align_ont.nf @@ -42,7 +42,6 @@ workflow ALIGN_ONT { // Convert merged BAM to CRAM and calculate indices and statistics SAMTOOLS_MERGE.out.bam | mix ( ch_bams.single_bam ) - | map { meta, bam -> [ meta, bam, [] ] } | set { ch_sort } diff --git a/subworkflows/local/align_pacbio.nf b/subworkflows/local/align_pacbio.nf index 07855a7..73a8321 100644 --- a/subworkflows/local/align_pacbio.nf +++ b/subworkflows/local/align_pacbio.nf @@ -49,7 +49,6 @@ workflow ALIGN_PACBIO { // Convert merged BAM to CRAM and calculate indices and statistics SAMTOOLS_MERGE.out.bam | mix ( ch_bams.single_bam ) - | map { meta, bam -> [ meta, bam, [] ] } | set { ch_sort } diff --git a/subworkflows/local/align_short.nf b/subworkflows/local/align_short.nf index 33c27b6..5b7017a 100644 --- a/subworkflows/local/align_short.nf +++ b/subworkflows/local/align_short.nf @@ -58,8 +58,9 @@ workflow ALIGN_SHORT { // Convert merged BAM to CRAM and calculate indices and statistics + SAMTOOLS_SORMADUP.out.bam.view() SAMTOOLS_SORMADUP.out.bam - | map { meta, bam -> [ meta, bam, [] ] } + | map { meta, bam -> [ meta, bam] } | set { ch_stat } diff --git a/subworkflows/local/convert_stats.nf b/subworkflows/local/convert_stats.nf index b89928a..3061bf7 100644 --- a/subworkflows/local/convert_stats.nf +++ b/subworkflows/local/convert_stats.nf @@ -21,7 +21,7 @@ workflow CONVERT_STATS { // Compress the quality scores of Illumina and PacBio CCS alignments bam | branch { - meta, bam, bai -> + meta, bam -> run_crumble : meta.datatype == "hic" || meta.datatype == "illumina" || meta.datatype == "pacbio" [meta, bam] no_crumble: true @@ -35,7 +35,9 @@ workflow CONVERT_STATS { // Convert BAM to CRAM CRUMBLE.out.bam | map { meta, bam -> [meta, bam, []] } - | mix ( ch_bams.no_crumble ) + | mix ( ch_bams.no_crumble.map{ + meta, bam -> [meta, bam, []] } + ) | set { ch_bams_for_conversion } SAMTOOLS_VIEW ( ch_bams_for_conversion, fasta, [] ) From db5450110e152950506596310e5b88b7325de96f Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 5 Jul 2024 18:55:14 +0100 Subject: [PATCH 039/111] cleanup un-needed line --- subworkflows/local/align_short.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/subworkflows/local/align_short.nf b/subworkflows/local/align_short.nf index 5b7017a..bb49e12 100644 --- a/subworkflows/local/align_short.nf +++ b/subworkflows/local/align_short.nf @@ -60,7 +60,6 @@ workflow ALIGN_SHORT { // Convert merged BAM to CRAM and calculate indices and statistics SAMTOOLS_SORMADUP.out.bam.view() SAMTOOLS_SORMADUP.out.bam - | map { meta, bam -> [ meta, bam] } | set { ch_stat } From 8b74d6c24bf3a10df85ad53498ac2242a8a66183 Mon Sep 17 00:00:00 2001 From: reichan1998 Date: Tue, 9 Jul 2024 16:00:07 +0700 Subject: [PATCH 040/111] add input type `fastq.gz` and `fg.gz` for Illumina reads --- docs/usage.md | 2 +- subworkflows/local/align_illumina_fastq.nf | 63 ++++++++++++++++++++++ workflows/readmapping.nf | 15 +++++- 3 files changed, 77 insertions(+), 3 deletions(-) create mode 100644 subworkflows/local/align_illumina_fastq.nf diff --git a/docs/usage.md b/docs/usage.md index 8c6923d..6922551 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -42,7 +42,7 @@ sample1_T5,pacbio,pacbio2.bam,pacbio2 | ---------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (\_). | | `datatype` | Type of sequencing data. Must be one of `hic`, `Illumina`, `pacbio`, or `ont`. | -| `datafile` | Full path to read data file. Must be `bam` or `cram` for `hic` and `illumina`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | +| `datafile` | Full path to read data file. Must be `bam` or `cram` for `hic`. Must be `bam`, `cram`, `fastq.gz` or `fq.gz` for `illumina`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | | `library` | (Optional) The library value is a unique identifier which is assigned to read group (`@RG`) ID. If the library name is not specified, the pipeline will auto-create library name using the data filename provided in the samplesheet. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. diff --git a/subworkflows/local/align_illumina_fastq.nf b/subworkflows/local/align_illumina_fastq.nf new file mode 100644 index 0000000..0447ea4 --- /dev/null +++ b/subworkflows/local/align_illumina_fastq.nf @@ -0,0 +1,63 @@ +// +// Align Illumina FASTQ data against the genome +// + +include { BWAMEM2_MEM } from '../../modules/nf-core/bwamem2/mem/main' +include { SAMTOOLS_MERGE } from '../../modules/nf-core/samtools/merge/main' +include { SAMTOOLS_SORMADUP } from '../../modules/local/samtools_sormadup' + + +workflow ALIGN_ILLUMINA_FASTQ { + take: + fasta // channel: [ val(meta), /path/to/fasta ] + index // channel: [ val(meta), /path/to/bwamem2/ ] + reads // channel: [ val(meta), /path/to/datafile ] + + + main: + ch_versions = Channel.empty() + + + // Align Fastq to Genome and output sorted BAM + BWAMEM2_MEM ( reads, index, true ) + ch_versions = ch_versions.mix ( BWAMEM2_MEM.out.versions.first() ) + + + // Collect all BWAMEM2 output by sample name + BWAMEM2_MEM.out.bam + | map { meta, bam -> [['id': meta.id.split('_')[0..-2].join('_'), 'datatype': meta.datatype], meta.read_count, bam] } + | groupTuple( by: [0] ) + | map { meta, read_counts, bams -> [meta + [read_count: read_counts.sum()], bams] } + | branch { + meta, bams -> + single_bam: bams.size() == 1 + multi_bams: true + } + | set { ch_bams } + + + // Merge, but only if there is more than 1 file + SAMTOOLS_MERGE ( ch_bams.multi_bams, [ [], [] ], [ [], [] ] ) + ch_versions = ch_versions.mix ( SAMTOOLS_MERGE.out.versions.first() ) + + + SAMTOOLS_MERGE.out.bam + | mix ( ch_bams.single_bam ) + | set { ch_bam } + + + // Mark duplicates + SAMTOOLS_SORMADUP ( ch_bam, fasta ) + ch_versions = ch_versions.mix ( SAMTOOLS_SORMADUP.out.versions ) + + + // Convert merged BAM to CRAM and calculate indices and statistics + SAMTOOLS_SORMADUP.out.bam + | map { meta, bam -> [ meta, bam, [] ] } + | set { ch_stat } + + + emit: + bam = ch_stat // channel: [ val(meta), /path/to/bam ] + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index 18ebd36..3623f22 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -33,6 +33,7 @@ include { ALIGN_PACBIO as ALIGN_HIFI } from '../subworkflows/local/align_pacb include { ALIGN_PACBIO as ALIGN_CLR } from '../subworkflows/local/align_pacbio' include { ALIGN_ONT } from '../subworkflows/local/align_ont' include { CONVERT_STATS } from '../subworkflows/local/convert_stats' +include { ALIGN_ILLUMINA_FASTQ } from '../subworkflows/local/align_illumina_fastq' /* @@ -76,6 +77,13 @@ workflow READMAPPING { ch_versions = ch_versions.mix ( INPUT_CHECK.out.versions ) + ch_reads.illumina + | branch { + meta, reads -> + fastq : reads.findAll { it.getName().toLowerCase() =~ /.*f.*\.gz/ } + cram : reads.findAll { it.getName().toLowerCase() =~ /.*cram/ } + } + | set { ch_illumina } // // SUBWORKFLOW: Uncompress and prepare reference genome files @@ -112,10 +120,13 @@ workflow READMAPPING { // ALIGN_HIC ( PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.bwaidx, ch_reads.hic ) ch_versions = ch_versions.mix ( ALIGN_HIC.out.versions ) - - ALIGN_ILLUMINA ( PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.bwaidx, ch_reads.illumina ) + + ALIGN_ILLUMINA ( PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.bwaidx, ch_illumina.cram ) ch_versions = ch_versions.mix ( ALIGN_ILLUMINA.out.versions ) + ALIGN_ILLUMINA_FASTQ ( PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.bwaidx, ch_illumina.fastq ) + ch_versions = ch_versions.mix ( ALIGN_ILLUMINA_FASTQ.out.versions ) + ALIGN_HIFI ( PREPARE_GENOME.out.fasta, ch_reads.pacbio, ch_vector_db ) ch_versions = ch_versions.mix ( ALIGN_HIFI.out.versions ) From e5a4e78ada056df7d135a7061d8d5c8f16554757 Mon Sep 17 00:00:00 2001 From: reichan1998 Date: Tue, 9 Jul 2024 16:26:08 +0700 Subject: [PATCH 041/111] prettier linting --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index 6922551..de7e25b 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -42,7 +42,7 @@ sample1_T5,pacbio,pacbio2.bam,pacbio2 | ---------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (\_). | | `datatype` | Type of sequencing data. Must be one of `hic`, `Illumina`, `pacbio`, or `ont`. | -| `datafile` | Full path to read data file. Must be `bam` or `cram` for `hic`. Must be `bam`, `cram`, `fastq.gz` or `fq.gz` for `illumina`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | +| `datafile` | Full path to read data file. Must be `bam` or `cram` for `hic`. Must be `bam`, `cram`, `fastq.gz` or `fq.gz` for `illumina`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | | `library` | (Optional) The library value is a unique identifier which is assigned to read group (`@RG`) ID. If the library name is not specified, the pipeline will auto-create library name using the data filename provided in the samplesheet. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. From d05026725e7d5d117c97c5a98b195addd3c84070 Mon Sep 17 00:00:00 2001 From: reichan1998 Date: Tue, 9 Jul 2024 16:35:48 +0700 Subject: [PATCH 042/111] prettier formating --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index de7e25b..d0c0faf 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -42,7 +42,7 @@ sample1_T5,pacbio,pacbio2.bam,pacbio2 | ---------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (\_). | | `datatype` | Type of sequencing data. Must be one of `hic`, `Illumina`, `pacbio`, or `ont`. | -| `datafile` | Full path to read data file. Must be `bam` or `cram` for `hic`. Must be `bam`, `cram`, `fastq.gz` or `fq.gz` for `illumina`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | +| `datafile` | Full path to read data file. Must be `bam` or `cram` for `hic`. Must be `bam` or `cram` or `fastq.gz` or `fq.gz` for `Illumina`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | | `library` | (Optional) The library value is a unique identifier which is assigned to read group (`@RG`) ID. If the library name is not specified, the pipeline will auto-create library name using the data filename provided in the samplesheet. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. From 4d3b4c208a3f11d5077b7a583f65c88c32aa16f4 Mon Sep 17 00:00:00 2001 From: reichan1998 Date: Tue, 9 Jul 2024 16:54:51 +0700 Subject: [PATCH 043/111] update usage.md --- docs/usage.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index d0c0faf..630e19d 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -38,11 +38,11 @@ sample1_T4,pacbio,pacbio1.bam,pacbio1 sample1_T5,pacbio,pacbio2.bam,pacbio2 ``` -| Column | Description | +| Column | Description | | ---------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (\_). | | `datatype` | Type of sequencing data. Must be one of `hic`, `Illumina`, `pacbio`, or `ont`. | -| `datafile` | Full path to read data file. Must be `bam` or `cram` for `hic`. Must be `bam` or `cram` or `fastq.gz` or `fq.gz` for `Illumina`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | +| `datafile` | Full path to read data file. Must be `bam` or `cram` for `hic`. Must be `cram` or `fastq.gz` or `fq.gz` for `Illumina`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | | `library` | (Optional) The library value is a unique identifier which is assigned to read group (`@RG`) ID. If the library name is not specified, the pipeline will auto-create library name using the data filename provided in the samplesheet. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. From dce6de722a02746c162461d942ce6c5ecc10cf92 Mon Sep 17 00:00:00 2001 From: reichan1998 Date: Tue, 9 Jul 2024 17:34:29 +0700 Subject: [PATCH 044/111] prettier linting --- docs/usage.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 630e19d..cb0fb20 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -38,11 +38,11 @@ sample1_T4,pacbio,pacbio1.bam,pacbio1 sample1_T5,pacbio,pacbio2.bam,pacbio2 ``` -| Column | Description | +| Column | Description | | ---------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (\_). | | `datatype` | Type of sequencing data. Must be one of `hic`, `Illumina`, `pacbio`, or `ont`. | -| `datafile` | Full path to read data file. Must be `bam` or `cram` for `hic`. Must be `cram` or `fastq.gz` or `fq.gz` for `Illumina`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | +| `datafile` | Full path to read data file. Must be `bam` or `cram` for `hic`. Must be `cram` or `fastq.gz` or `fq.gz` for `Illumina`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | | `library` | (Optional) The library value is a unique identifier which is assigned to read group (`@RG`) ID. If the library name is not specified, the pipeline will auto-create library name using the data filename provided in the samplesheet. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. From 480430727fcb6b7ce96d9727b59e902a8d866480 Mon Sep 17 00:00:00 2001 From: reichan1998 Date: Tue, 9 Jul 2024 19:08:20 +0700 Subject: [PATCH 045/111] move the branching for FASTQ files inside of align_short.nf --- docs/usage.md | 2 +- subworkflows/local/align_illumina_fastq.nf | 63 ---------------------- subworkflows/local/align_short.nf | 28 +++++++--- workflows/readmapping.nf | 13 +---- 4 files changed, 24 insertions(+), 82 deletions(-) delete mode 100644 subworkflows/local/align_illumina_fastq.nf diff --git a/docs/usage.md b/docs/usage.md index cb0fb20..dc201ef 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -42,7 +42,7 @@ sample1_T5,pacbio,pacbio2.bam,pacbio2 | ---------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (\_). | | `datatype` | Type of sequencing data. Must be one of `hic`, `Illumina`, `pacbio`, or `ont`. | -| `datafile` | Full path to read data file. Must be `bam` or `cram` for `hic`. Must be `cram` or `fastq.gz` or `fq.gz` for `Illumina`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | +| `datafile` | Full path to read data file. Must be `bam` or `cram` or `fastq.gz` or `fq.gz` for `Illumina` and `HiC`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | | `library` | (Optional) The library value is a unique identifier which is assigned to read group (`@RG`) ID. If the library name is not specified, the pipeline will auto-create library name using the data filename provided in the samplesheet. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. diff --git a/subworkflows/local/align_illumina_fastq.nf b/subworkflows/local/align_illumina_fastq.nf deleted file mode 100644 index 0447ea4..0000000 --- a/subworkflows/local/align_illumina_fastq.nf +++ /dev/null @@ -1,63 +0,0 @@ -// -// Align Illumina FASTQ data against the genome -// - -include { BWAMEM2_MEM } from '../../modules/nf-core/bwamem2/mem/main' -include { SAMTOOLS_MERGE } from '../../modules/nf-core/samtools/merge/main' -include { SAMTOOLS_SORMADUP } from '../../modules/local/samtools_sormadup' - - -workflow ALIGN_ILLUMINA_FASTQ { - take: - fasta // channel: [ val(meta), /path/to/fasta ] - index // channel: [ val(meta), /path/to/bwamem2/ ] - reads // channel: [ val(meta), /path/to/datafile ] - - - main: - ch_versions = Channel.empty() - - - // Align Fastq to Genome and output sorted BAM - BWAMEM2_MEM ( reads, index, true ) - ch_versions = ch_versions.mix ( BWAMEM2_MEM.out.versions.first() ) - - - // Collect all BWAMEM2 output by sample name - BWAMEM2_MEM.out.bam - | map { meta, bam -> [['id': meta.id.split('_')[0..-2].join('_'), 'datatype': meta.datatype], meta.read_count, bam] } - | groupTuple( by: [0] ) - | map { meta, read_counts, bams -> [meta + [read_count: read_counts.sum()], bams] } - | branch { - meta, bams -> - single_bam: bams.size() == 1 - multi_bams: true - } - | set { ch_bams } - - - // Merge, but only if there is more than 1 file - SAMTOOLS_MERGE ( ch_bams.multi_bams, [ [], [] ], [ [], [] ] ) - ch_versions = ch_versions.mix ( SAMTOOLS_MERGE.out.versions.first() ) - - - SAMTOOLS_MERGE.out.bam - | mix ( ch_bams.single_bam ) - | set { ch_bam } - - - // Mark duplicates - SAMTOOLS_SORMADUP ( ch_bam, fasta ) - ch_versions = ch_versions.mix ( SAMTOOLS_SORMADUP.out.versions ) - - - // Convert merged BAM to CRAM and calculate indices and statistics - SAMTOOLS_SORMADUP.out.bam - | map { meta, bam -> [ meta, bam, [] ] } - | set { ch_stat } - - - emit: - bam = ch_stat // channel: [ val(meta), /path/to/bam ] - versions = ch_versions // channel: [ versions.yml ] -} diff --git a/subworkflows/local/align_short.nf b/subworkflows/local/align_short.nf index 33c27b6..3237a1e 100644 --- a/subworkflows/local/align_short.nf +++ b/subworkflows/local/align_short.nf @@ -18,15 +18,31 @@ workflow ALIGN_SHORT { main: ch_versions = Channel.empty() + // Check file types and branch + reads + | branch { + meta, reads -> + fastq : reads.findAll { it.getName().toLowerCase() =~ /.*f.*\.gz/ } + cram : true + } + | set { ch_reads } + - // Convert from CRAM to FASTQ - SAMTOOLS_FASTQ ( reads, false ) - ch_versions = ch_versions.mix ( SAMTOOLS_FASTQ.out.versions.first() ) + if ( ch_reads.cram ) { + // Convert from CRAM to FASTQ + SAMTOOLS_FASTQ ( ch_reads.cram, false ) + ch_versions = ch_versions.mix ( SAMTOOLS_FASTQ.out.versions.first() ) - // Align Fastq to Genome and output sorted BAM - BWAMEM2_MEM ( SAMTOOLS_FASTQ.out.fastq, index, true ) - ch_versions = ch_versions.mix ( BWAMEM2_MEM.out.versions.first() ) + // Align Fastq to Genome and output sorted BAM + BWAMEM2_MEM ( SAMTOOLS_FASTQ.out.fastq, index, true ) + ch_versions = ch_versions.mix ( BWAMEM2_MEM.out.versions.first() ) + + + } else { + BWAMEM2_MEM ( ch_reads.fastq, index, true ) + ch_versions = ch_versions.mix ( BWAMEM2_MEM.out.versions.first() ) + } // Collect all BWAMEM2 output by sample name diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index 3623f22..2910f52 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -33,7 +33,6 @@ include { ALIGN_PACBIO as ALIGN_HIFI } from '../subworkflows/local/align_pacb include { ALIGN_PACBIO as ALIGN_CLR } from '../subworkflows/local/align_pacbio' include { ALIGN_ONT } from '../subworkflows/local/align_ont' include { CONVERT_STATS } from '../subworkflows/local/convert_stats' -include { ALIGN_ILLUMINA_FASTQ } from '../subworkflows/local/align_illumina_fastq' /* @@ -77,13 +76,6 @@ workflow READMAPPING { ch_versions = ch_versions.mix ( INPUT_CHECK.out.versions ) - ch_reads.illumina - | branch { - meta, reads -> - fastq : reads.findAll { it.getName().toLowerCase() =~ /.*f.*\.gz/ } - cram : reads.findAll { it.getName().toLowerCase() =~ /.*cram/ } - } - | set { ch_illumina } // // SUBWORKFLOW: Uncompress and prepare reference genome files @@ -121,12 +113,9 @@ workflow READMAPPING { ALIGN_HIC ( PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.bwaidx, ch_reads.hic ) ch_versions = ch_versions.mix ( ALIGN_HIC.out.versions ) - ALIGN_ILLUMINA ( PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.bwaidx, ch_illumina.cram ) + ALIGN_ILLUMINA ( PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.bwaidx, ch_reads.illumina ) ch_versions = ch_versions.mix ( ALIGN_ILLUMINA.out.versions ) - ALIGN_ILLUMINA_FASTQ ( PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.bwaidx, ch_illumina.fastq ) - ch_versions = ch_versions.mix ( ALIGN_ILLUMINA_FASTQ.out.versions ) - ALIGN_HIFI ( PREPARE_GENOME.out.fasta, ch_reads.pacbio, ch_vector_db ) ch_versions = ch_versions.mix ( ALIGN_HIFI.out.versions ) From e3e27ae1e774a98a1cc6e5afc86fea2df0494d91 Mon Sep 17 00:00:00 2001 From: reichan1998 Date: Tue, 9 Jul 2024 21:59:40 +0700 Subject: [PATCH 046/111] edit align_short.nf --- subworkflows/local/align_short.nf | 28 +++++++++++++--------------- 1 file changed, 13 insertions(+), 15 deletions(-) diff --git a/subworkflows/local/align_short.nf b/subworkflows/local/align_short.nf index 3237a1e..f318606 100644 --- a/subworkflows/local/align_short.nf +++ b/subworkflows/local/align_short.nf @@ -28,21 +28,19 @@ workflow ALIGN_SHORT { | set { ch_reads } - if ( ch_reads.cram ) { - // Convert from CRAM to FASTQ - SAMTOOLS_FASTQ ( ch_reads.cram, false ) - ch_versions = ch_versions.mix ( SAMTOOLS_FASTQ.out.versions.first() ) - - - // Align Fastq to Genome and output sorted BAM - BWAMEM2_MEM ( SAMTOOLS_FASTQ.out.fastq, index, true ) - ch_versions = ch_versions.mix ( BWAMEM2_MEM.out.versions.first() ) - - - } else { - BWAMEM2_MEM ( ch_reads.fastq, index, true ) - ch_versions = ch_versions.mix ( BWAMEM2_MEM.out.versions.first() ) - } + // Convert from CRAM to FASTQ only if CRAM files were provided as input + SAMTOOLS_FASTQ ( ch_reads.cram, false ) + ch_versions = ch_versions.mix ( SAMTOOLS_FASTQ.out.versions.first() ) + + + SAMTOOLS_FASTQ.out.fastq + | mix ( ch_reads.fastq ) + | set { ch_reads_fastq } + + + // Align Fastq to Genome and output sorted BAM + BWAMEM2_MEM ( ch_reads_fastq, index, true ) + ch_versions = ch_versions.mix ( BWAMEM2_MEM.out.versions.first() ) // Collect all BWAMEM2 output by sample name From f5b3edc11ad9fdbbe17001f9d4778dfbfcc18970 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Thu, 11 Jul 2024 16:49:16 +0100 Subject: [PATCH 047/111] Update subworkflows/local/align_short.nf Co-authored-by: Matthieu Muffato --- subworkflows/local/align_short.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/subworkflows/local/align_short.nf b/subworkflows/local/align_short.nf index bb49e12..09befb8 100644 --- a/subworkflows/local/align_short.nf +++ b/subworkflows/local/align_short.nf @@ -58,7 +58,6 @@ workflow ALIGN_SHORT { // Convert merged BAM to CRAM and calculate indices and statistics - SAMTOOLS_SORMADUP.out.bam.view() SAMTOOLS_SORMADUP.out.bam | set { ch_stat } From a7c125b61a5e6a3d49bb519e5665c0cf99f69e05 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Thu, 11 Jul 2024 16:51:49 +0100 Subject: [PATCH 048/111] Update modules/local/samtools_replaceheader.nf Co-authored-by: Matthieu Muffato --- modules/local/samtools_replaceheader.nf | 16 ++++++++-------- 1 file changed, 8 insertions(+), 8 deletions(-) diff --git a/modules/local/samtools_replaceheader.nf b/modules/local/samtools_replaceheader.nf index 06e45f6..0a027c8 100644 --- a/modules/local/samtools_replaceheader.nf +++ b/modules/local/samtools_replaceheader.nf @@ -27,18 +27,18 @@ process SAMTOOLS_REHEADER { """ # Replace SQ lines with those from external template ( samtools view --no-PG --header-only ${file} | \\ - grep -v ^@SQ && grep ^@SQ ${header} ) > .temp.header.sam + grep -v ^@SQ && grep ^@SQ ${header} ) > temp.header.sam # custom sort for readability (retain order of insertion but sort groups by tag) - ( grep ^@HD .temp.header.sam || true && \ - grep ^@SQ .temp.header.sam || true && \ - grep ^@RG .temp.header.sam || true && \ - grep ^@PG .temp.header.sam || true && \ - grep -v -E '^@HD|^@SQ|^@RG|^@PG' .temp.header.sam || true; \ - ) > .temp.sorted.header.sam + ( grep ^@HD temp.header.sam || true && \ + grep ^@SQ temp.header.sam || true && \ + grep ^@RG temp.header.sam || true && \ + grep ^@PG temp.header.sam || true && \ + grep -v -E '^@HD|^@SQ|^@RG|^@PG' temp.header.sam || true; \ + ) > temp.sorted.header.sam # Insert new header into file - samtools reheader .temp.sorted.header.sam ${file} > ${prefix}.${suffix} + samtools reheader temp.sorted.header.sam ${file} > ${prefix}.${suffix} cat <<-END_VERSIONS > versions.yml "${task.process}": From 7a714fcc5e9b329c8b7acfb61a010b7e93d1512e Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Thu, 11 Jul 2024 16:52:46 +0100 Subject: [PATCH 049/111] Update subworkflows/local/convert_stats.nf Co-authored-by: Matthieu Muffato --- subworkflows/local/convert_stats.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/subworkflows/local/convert_stats.nf b/subworkflows/local/convert_stats.nf index 3061bf7..dce58c7 100644 --- a/subworkflows/local/convert_stats.nf +++ b/subworkflows/local/convert_stats.nf @@ -23,7 +23,6 @@ workflow CONVERT_STATS { | branch { meta, bam -> run_crumble : meta.datatype == "hic" || meta.datatype == "illumina" || meta.datatype == "pacbio" - [meta, bam] no_crumble: true } | set { ch_bams } From ca86a50171b9f3ec9be885e25f6fa20182728843 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Thu, 11 Jul 2024 16:53:41 +0100 Subject: [PATCH 050/111] Update modules/local/samtools_replaceheader.nf Co-authored-by: Matthieu Muffato --- modules/local/samtools_replaceheader.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/samtools_replaceheader.nf b/modules/local/samtools_replaceheader.nf index 0a027c8..09ac4fc 100644 --- a/modules/local/samtools_replaceheader.nf +++ b/modules/local/samtools_replaceheader.nf @@ -12,8 +12,8 @@ process SAMTOOLS_REHEADER { path(header) output: - tuple val(meta), path("${prefix}.${suffix}"), optional:true, emit: bam - tuple val(meta), path("${prefix}.${suffix}"), optional:true, emit: cram + tuple val(meta), path("${prefix}.bam") , optional:true, emit: bam + tuple val(meta), path("${prefix}.cram"), optional:true, emit: cram path "versions.yml", emit: versions when: From 15e8d7b2a6a7aa9cd78ea1e907d8d6ae69f9cfb9 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 12 Jul 2024 09:08:41 +0100 Subject: [PATCH 051/111] streamline prep for samtools_view call --- subworkflows/local/convert_stats.nf | 4 +--- 1 file changed, 1 insertion(+), 3 deletions(-) diff --git a/subworkflows/local/convert_stats.nf b/subworkflows/local/convert_stats.nf index dce58c7..66308e0 100644 --- a/subworkflows/local/convert_stats.nf +++ b/subworkflows/local/convert_stats.nf @@ -33,10 +33,8 @@ workflow CONVERT_STATS { // Convert BAM to CRAM CRUMBLE.out.bam + | mix ( ch_bams.no_crumble ) | map { meta, bam -> [meta, bam, []] } - | mix ( ch_bams.no_crumble.map{ - meta, bam -> [meta, bam, []] } - ) | set { ch_bams_for_conversion } SAMTOOLS_VIEW ( ch_bams_for_conversion, fasta, [] ) From 3aca264d6c0b714d5ce19968201f2f3bb3295ea5 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 12 Jul 2024 09:09:46 +0100 Subject: [PATCH 052/111] remove intermediate steps before emit --- subworkflows/local/align_short.nf | 8 +------- 1 file changed, 1 insertion(+), 7 deletions(-) diff --git a/subworkflows/local/align_short.nf b/subworkflows/local/align_short.nf index 09befb8..8f061ad 100644 --- a/subworkflows/local/align_short.nf +++ b/subworkflows/local/align_short.nf @@ -56,13 +56,7 @@ workflow ALIGN_SHORT { SAMTOOLS_SORMADUP ( ch_bam, fasta ) ch_versions = ch_versions.mix ( SAMTOOLS_SORMADUP.out.versions ) - - // Convert merged BAM to CRAM and calculate indices and statistics - SAMTOOLS_SORMADUP.out.bam - | set { ch_stat } - - emit: - bam = ch_stat // channel: [ val(meta), /path/to/bam ] + bam = SAMTOOLS_SORMADUP.out.bam // channel: [ val(meta), /path/to/bam ] versions = ch_versions // channel: [ versions.yml ] } From 539a2fda41553e0b3567c6ac21238d43210e527c Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 17 Jul 2024 11:10:37 +0100 Subject: [PATCH 053/111] nf-core modules update and necessary integrations --- .github/PULL_REQUEST_TEMPLATE.md | 1 + .github/workflows/linting.yml | 68 +-- modules.json | 30 +- modules/nf-core/blast/blastn/environment.yml | 7 + modules/nf-core/blast/blastn/main.nf | 49 +- modules/nf-core/blast/blastn/meta.yml | 20 +- .../nf-core/blast/blastn/tests/main.nf.test | 72 +++ .../blast/blastn/tests/main.nf.test.snap | 18 + .../blast/blastn/tests/nextflow.config | 5 + modules/nf-core/blast/blastn/tests/tags.yml | 2 + modules/nf-core/bwamem2/index/environment.yml | 7 + modules/nf-core/bwamem2/index/main.nf | 17 +- modules/nf-core/bwamem2/index/meta.yml | 2 + .../nf-core/bwamem2/index/tests/main.nf.test | 31 ++ .../bwamem2/index/tests/main.nf.test.snap | 47 ++ modules/nf-core/bwamem2/index/tests/tags.yml | 2 + modules/nf-core/bwamem2/mem/environment.yml | 10 + modules/nf-core/bwamem2/mem/main.nf | 45 +- modules/nf-core/bwamem2/mem/meta.yml | 29 + .../nf-core/bwamem2/mem/tests/main.nf.test | 234 ++++++++ .../bwamem2/mem/tests/main.nf.test.snap | 67 +++ modules/nf-core/bwamem2/mem/tests/tags.yml | 2 + modules/nf-core/crumble/environment.yml | 7 + modules/nf-core/crumble/main.nf | 2 +- modules/nf-core/crumble/meta.yml | 5 +- .../dumpsoftwareversions/environment.yml | 7 + .../custom/dumpsoftwareversions/main.nf | 6 +- .../custom/dumpsoftwareversions/meta.yml | 7 +- .../templates/dumpsoftwareversions.py | 1 - .../dumpsoftwareversions/tests/main.nf.test | 43 ++ .../tests/main.nf.test.snap | 33 ++ .../dumpsoftwareversions/tests/tags.yml | 2 + modules/nf-core/gunzip/environment.yml | 9 + modules/nf-core/gunzip/main.nf | 29 +- modules/nf-core/gunzip/meta.yml | 5 + modules/nf-core/gunzip/tests/main.nf.test | 121 +++++ .../nf-core/gunzip/tests/main.nf.test.snap | 134 +++++ modules/nf-core/gunzip/tests/nextflow.config | 5 + modules/nf-core/gunzip/tests/tags.yml | 2 + .../nf-core/minimap2/align/environment.yml | 8 +- modules/nf-core/minimap2/align/main.nf | 36 +- modules/nf-core/minimap2/align/meta.yml | 9 + .../nf-core/minimap2/align/tests/main.nf.test | 137 ++++- .../minimap2/align/tests/main.nf.test.snap | 192 ++++++- .../nf-core/samtools/faidx/environment.yml | 10 + modules/nf-core/samtools/faidx/main.nf | 6 +- modules/nf-core/samtools/faidx/meta.yml | 8 + .../nf-core/samtools/faidx/tests/main.nf.test | 122 +++++ .../samtools/faidx/tests/main.nf.test.snap | 249 +++++++++ .../samtools/faidx/tests/nextflow.config | 7 + .../samtools/faidx/tests/nextflow2.config | 6 + modules/nf-core/samtools/faidx/tests/tags.yml | 2 + .../nf-core/samtools/fastq/environment.yml | 8 + modules/nf-core/samtools/fastq/main.nf | 10 +- modules/nf-core/samtools/fastq/meta.yml | 6 +- .../nf-core/samtools/fastq/tests/main.nf.test | 67 +++ .../samtools/fastq/tests/main.nf.test.snap | 139 +++++ modules/nf-core/samtools/fastq/tests/tags.yml | 2 + .../nf-core/samtools/flagstat/environment.yml | 8 + modules/nf-core/samtools/flagstat/main.nf | 17 +- modules/nf-core/samtools/flagstat/meta.yml | 2 + .../samtools/flagstat/tests/main.nf.test | 36 ++ .../samtools/flagstat/tests/main.nf.test.snap | 32 ++ .../nf-core/samtools/flagstat/tests/tags.yml | 2 + .../nf-core/samtools/idxstats/environment.yml | 8 + modules/nf-core/samtools/idxstats/main.nf | 18 +- modules/nf-core/samtools/idxstats/meta.yml | 2 + .../samtools/idxstats/tests/main.nf.test | 36 ++ .../samtools/idxstats/tests/main.nf.test.snap | 32 ++ .../nf-core/samtools/idxstats/tests/tags.yml | 2 + .../nf-core/samtools/merge/environment.yml | 8 + modules/nf-core/samtools/merge/main.nf | 11 +- modules/nf-core/samtools/merge/meta.yml | 10 + .../samtools/merge/samtools-merge.diff | 4 +- .../nf-core/samtools/merge/tests/index.config | 3 + .../nf-core/samtools/merge/tests/main.nf.test | 137 +++++ .../samtools/merge/tests/main.nf.test.snap | 228 ++++++++ modules/nf-core/samtools/merge/tests/tags.yml | 2 + .../nf-core/samtools/stats/environment.yml | 8 + modules/nf-core/samtools/stats/main.nf | 6 +- modules/nf-core/samtools/stats/meta.yml | 4 + .../nf-core/samtools/stats/tests/main.nf.test | 65 +++ .../samtools/stats/tests/main.nf.test.snap | 72 +++ modules/nf-core/samtools/stats/tests/tags.yml | 2 + modules/nf-core/samtools/view/environment.yml | 8 + modules/nf-core/samtools/view/main.nf | 49 +- modules/nf-core/samtools/view/meta.yml | 14 + .../nf-core/samtools/view/tests/bam.config | 3 + .../samtools/view/tests/bam_index.config | 3 + .../nf-core/samtools/view/tests/main.nf.test | 214 ++++++++ .../samtools/view/tests/main.nf.test.snap | 508 ++++++++++++++++++ modules/nf-core/samtools/view/tests/tags.yml | 2 + modules/nf-core/untar/environment.yml | 9 + modules/nf-core/untar/main.nf | 31 +- modules/nf-core/untar/meta.yml | 5 + modules/nf-core/untar/tests/main.nf.test | 85 +++ modules/nf-core/untar/tests/main.nf.test.snap | 158 ++++++ modules/nf-core/untar/tests/tags.yml | 2 + subworkflows/local/align_ont.nf | 2 +- subworkflows/local/align_pacbio.nf | 3 +- subworkflows/local/align_short.nf | 2 +- workflows/readmapping.nf | 1 - 102 files changed, 3847 insertions(+), 214 deletions(-) create mode 100644 modules/nf-core/blast/blastn/environment.yml create mode 100644 modules/nf-core/blast/blastn/tests/main.nf.test create mode 100644 modules/nf-core/blast/blastn/tests/main.nf.test.snap create mode 100644 modules/nf-core/blast/blastn/tests/nextflow.config create mode 100644 modules/nf-core/blast/blastn/tests/tags.yml create mode 100644 modules/nf-core/bwamem2/index/environment.yml create mode 100644 modules/nf-core/bwamem2/index/tests/main.nf.test create mode 100644 modules/nf-core/bwamem2/index/tests/main.nf.test.snap create mode 100644 modules/nf-core/bwamem2/index/tests/tags.yml create mode 100644 modules/nf-core/bwamem2/mem/environment.yml create mode 100644 modules/nf-core/bwamem2/mem/tests/main.nf.test create mode 100644 modules/nf-core/bwamem2/mem/tests/main.nf.test.snap create mode 100644 modules/nf-core/bwamem2/mem/tests/tags.yml create mode 100644 modules/nf-core/crumble/environment.yml create mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml create mode 100644 modules/nf-core/gunzip/environment.yml create mode 100644 modules/nf-core/gunzip/tests/main.nf.test create mode 100644 modules/nf-core/gunzip/tests/main.nf.test.snap create mode 100644 modules/nf-core/gunzip/tests/nextflow.config create mode 100644 modules/nf-core/gunzip/tests/tags.yml create mode 100644 modules/nf-core/samtools/faidx/environment.yml create mode 100644 modules/nf-core/samtools/faidx/tests/main.nf.test create mode 100644 modules/nf-core/samtools/faidx/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/faidx/tests/nextflow.config create mode 100644 modules/nf-core/samtools/faidx/tests/nextflow2.config create mode 100644 modules/nf-core/samtools/faidx/tests/tags.yml create mode 100644 modules/nf-core/samtools/fastq/environment.yml create mode 100644 modules/nf-core/samtools/fastq/tests/main.nf.test create mode 100644 modules/nf-core/samtools/fastq/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/fastq/tests/tags.yml create mode 100644 modules/nf-core/samtools/flagstat/environment.yml create mode 100644 modules/nf-core/samtools/flagstat/tests/main.nf.test create mode 100644 modules/nf-core/samtools/flagstat/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/flagstat/tests/tags.yml create mode 100644 modules/nf-core/samtools/idxstats/environment.yml create mode 100644 modules/nf-core/samtools/idxstats/tests/main.nf.test create mode 100644 modules/nf-core/samtools/idxstats/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/idxstats/tests/tags.yml create mode 100644 modules/nf-core/samtools/merge/environment.yml create mode 100644 modules/nf-core/samtools/merge/tests/index.config create mode 100644 modules/nf-core/samtools/merge/tests/main.nf.test create mode 100644 modules/nf-core/samtools/merge/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/merge/tests/tags.yml create mode 100644 modules/nf-core/samtools/stats/environment.yml create mode 100644 modules/nf-core/samtools/stats/tests/main.nf.test create mode 100644 modules/nf-core/samtools/stats/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/stats/tests/tags.yml create mode 100644 modules/nf-core/samtools/view/environment.yml create mode 100644 modules/nf-core/samtools/view/tests/bam.config create mode 100644 modules/nf-core/samtools/view/tests/bam_index.config create mode 100644 modules/nf-core/samtools/view/tests/main.nf.test create mode 100644 modules/nf-core/samtools/view/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/view/tests/tags.yml create mode 100644 modules/nf-core/untar/environment.yml create mode 100644 modules/nf-core/untar/tests/main.nf.test create mode 100644 modules/nf-core/untar/tests/main.nf.test.snap create mode 100644 modules/nf-core/untar/tests/tags.yml diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index af196cc..6649492 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,6 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/sanger-tol/r - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/sanger-tol/readmapping/tree/master/.github/CONTRIBUTING.md) - [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. - [ ] `CHANGELOG.md` is updated. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 858d622..1fcafe8 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,74 +11,34 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - - uses: actions/setup-node@v3 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v3 - - - uses: actions/setup-node@v3 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v3 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.12 + uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: "3.12" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: "3.7" + python-version: "3.12" architecture: "x64" - name: Install dependencies @@ -99,7 +59,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4 with: name: linting-logs path: | diff --git a/modules.json b/modules.json index 81077ac..c995b54 100644 --- a/modules.json +++ b/modules.json @@ -7,78 +7,78 @@ "nf-core": { "blast/blastn": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "4262a04142431275e54e1f4b413628a2201ed6e6", "installed_by": ["modules"] }, "bwamem2/index": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "7081e04c18de9480948d34513a1c1e2d0fa9126d", "installed_by": ["modules"] }, "bwamem2/mem": { "branch": "master", - "git_sha": "0460d316170f75f323111b4a2c0a2989f0c32013", + "git_sha": "3afb95b2e15fc4a2347470255a7ef654f650c8ec", "installed_by": ["modules"] }, "crumble": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "82024cf6325d2ee194e7f056d841ecad2f6856e9", "installed_by": ["modules"] }, "gunzip": { "branch": "master", - "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", + "git_sha": "4e5f4687318f24ba944a13609d3ea6ebd890737d", "installed_by": ["modules"] }, "minimap2/align": { "branch": "master", - "git_sha": "efbf86bb487f288ac30660282709d9620dd6048e", + "git_sha": "e83b347b3e674de6bb1bb7bdf9b2674768c8e0fe", "installed_by": ["modules"] }, "samtools/faidx": { "branch": "master", - "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe", + "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", "installed_by": ["modules"] }, "samtools/fastq": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", "installed_by": ["modules"] }, "samtools/flagstat": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", "installed_by": ["modules"] }, "samtools/idxstats": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", "installed_by": ["modules"] }, "samtools/merge": { "branch": "master", - "git_sha": "0460d316170f75f323111b4a2c0a2989f0c32013", + "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", "installed_by": ["modules"], "patch": "modules/nf-core/samtools/merge/samtools-merge.diff" }, "samtools/stats": { "branch": "master", - "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", + "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", "installed_by": ["modules"] }, "samtools/view": { "branch": "master", - "git_sha": "3ffae3598260a99e8db3207dead9f73f87f90d1f", + "git_sha": "6c2309aaec566c0d44a6cf14d4b2d0c51afe2e91", "installed_by": ["modules"] }, "untar": { "branch": "master", - "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", + "git_sha": "4e5f4687318f24ba944a13609d3ea6ebd890737d", "installed_by": ["modules"] } } diff --git a/modules/nf-core/blast/blastn/environment.yml b/modules/nf-core/blast/blastn/environment.yml new file mode 100644 index 0000000..e4a7280 --- /dev/null +++ b/modules/nf-core/blast/blastn/environment.yml @@ -0,0 +1,7 @@ +name: blast_blastn +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::blast=2.15.0 diff --git a/modules/nf-core/blast/blastn/main.nf b/modules/nf-core/blast/blastn/main.nf index 9a1f3a5..68b43ba 100644 --- a/modules/nf-core/blast/blastn/main.nf +++ b/modules/nf-core/blast/blastn/main.nf @@ -2,18 +2,18 @@ process BLAST_BLASTN { tag "$meta.id" label 'process_medium' - conda "bioconda::blast=2.13.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/blast:2.13.0--hf3cf87c_0' : - 'biocontainers/blast:2.13.0--hf3cf87c_0' }" + 'https://depot.galaxyproject.org/singularity/blast:2.15.0--pl5321h6f7f691_1': + 'biocontainers/blast:2.15.0--pl5321h6f7f691_1' }" input: - tuple val(meta), path(fasta) - path db + tuple val(meta) , path(fasta) + tuple val(meta2), path(db) output: - tuple val(meta), path('*.blastn.txt'), emit: txt - path "versions.yml" , emit: versions + tuple val(meta), path('*.txt'), emit: txt + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -21,14 +21,39 @@ process BLAST_BLASTN { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + def is_compressed = fasta.getExtension() == "gz" ? true : false + def fasta_name = is_compressed ? fasta.getBaseName() : fasta + """ - DB=`find -L ./ -name "*.ndb" | sed 's/\\.ndb\$//'` + if [ "${is_compressed}" == "true" ]; then + gzip -c -d ${fasta} > ${fasta_name} + fi + + DB=`find -L ./ -name "*.nal" | sed 's/\\.nal\$//'` + if [ -z "\$DB" ]; then + DB=`find -L ./ -name "*.nin" | sed 's/\\.nin\$//'` + fi + echo Using \$DB + blastn \\ - -num_threads $task.cpus \\ + -num_threads ${task.cpus} \\ -db \$DB \\ - -query $fasta \\ - $args \\ - -out ${prefix}.blastn.txt + -query ${fasta_name} \\ + ${args} \\ + -out ${prefix}.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + blast: \$(blastn -version 2>&1 | sed 's/^.*blastn: //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.txt + cat <<-END_VERSIONS > versions.yml "${task.process}": blast: \$(blastn -version 2>&1 | sed 's/^.*blastn: //; s/ .*\$//') diff --git a/modules/nf-core/blast/blastn/meta.yml b/modules/nf-core/blast/blastn/meta.yml index 2742278..a0d64dd 100644 --- a/modules/nf-core/blast/blastn/meta.yml +++ b/modules/nf-core/blast/blastn/meta.yml @@ -22,16 +22,26 @@ input: - fasta: type: file description: Input fasta file containing queries sequences - pattern: "*.{fa,fasta}" + pattern: "*.{fa,fasta,fa.gz,fasta.gz}" + - meta2: + type: map + description: | + Groovy Map containing db information + e.g. [ id:'test2', single_end:false ] - db: type: directory - description: Directory containing blast database + description: Directory containing the blast database pattern: "*" output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] - txt: type: file description: File containing blastn hits - pattern: "*.{blastn.txt}" + pattern: "*.txt" - versions: type: file description: File containing software versions @@ -39,3 +49,7 @@ output: authors: - "@joseespinosa" - "@drpatelh" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@vagkaratzas" diff --git a/modules/nf-core/blast/blastn/tests/main.nf.test b/modules/nf-core/blast/blastn/tests/main.nf.test new file mode 100644 index 0000000..02ecfab --- /dev/null +++ b/modules/nf-core/blast/blastn/tests/main.nf.test @@ -0,0 +1,72 @@ +nextflow_process { + + name "Test Process BLAST_BLASTN" + script "../main.nf" + process "BLAST_BLASTN" + config "./nextflow.config" + tag "modules" + tag "modules_nfcore" + tag "blast" + tag "blast/blastn" + tag "blast/makeblastdb" + + setup { + run("BLAST_MAKEBLASTDB") { + script "../../makeblastdb/main.nf" + process { + """ + input[0] = [ [id:'test2'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + """ + } + } + } + + test("Should search for nucleotide hits against a blast db") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ [id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + input[1] = BLAST_MAKEBLASTDB.out.db + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.txt[0][1]).getText().contains("Query= MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate") }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("Should search for zipped nucleotide hits against a blast db") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ [id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta_gz'], checkIfExists: true) ] + input[1] = BLAST_MAKEBLASTDB.out.db + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.txt[0][1]).getText().contains("Query= MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate") }, + { assert snapshot(process.out.versions).match("versions_zipped") } + ) + } + + } + +} diff --git a/modules/nf-core/blast/blastn/tests/main.nf.test.snap b/modules/nf-core/blast/blastn/tests/main.nf.test.snap new file mode 100644 index 0000000..dd8b775 --- /dev/null +++ b/modules/nf-core/blast/blastn/tests/main.nf.test.snap @@ -0,0 +1,18 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,faf2471d836ebbf24d96d3e1f8720b17" + ] + ], + "timestamp": "2023-12-11T07:20:03.54997013" + }, + "versions_zipped": { + "content": [ + [ + "versions.yml:md5,faf2471d836ebbf24d96d3e1f8720b17" + ] + ], + "timestamp": "2023-12-11T07:20:12.925782708" + } +} \ No newline at end of file diff --git a/modules/nf-core/blast/blastn/tests/nextflow.config b/modules/nf-core/blast/blastn/tests/nextflow.config new file mode 100644 index 0000000..0899289 --- /dev/null +++ b/modules/nf-core/blast/blastn/tests/nextflow.config @@ -0,0 +1,5 @@ +process { + withName: BLAST_MAKEBLASTDB { + ext.args = '-dbtype nucl' + } +} diff --git a/modules/nf-core/blast/blastn/tests/tags.yml b/modules/nf-core/blast/blastn/tests/tags.yml new file mode 100644 index 0000000..b4588ab --- /dev/null +++ b/modules/nf-core/blast/blastn/tests/tags.yml @@ -0,0 +1,2 @@ +blast/blastn: + - modules/nf-core/blast/blastn/** diff --git a/modules/nf-core/bwamem2/index/environment.yml b/modules/nf-core/bwamem2/index/environment.yml new file mode 100644 index 0000000..26b4391 --- /dev/null +++ b/modules/nf-core/bwamem2/index/environment.yml @@ -0,0 +1,7 @@ +name: bwamem2_index +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bwa-mem2=2.2.1 diff --git a/modules/nf-core/bwamem2/index/main.nf b/modules/nf-core/bwamem2/index/main.nf index 3094085..b768828 100644 --- a/modules/nf-core/bwamem2/index/main.nf +++ b/modules/nf-core/bwamem2/index/main.nf @@ -2,7 +2,7 @@ process BWAMEM2_INDEX { tag "$fasta" label 'process_single' - conda "bioconda::bwa-mem2=2.2.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bwa-mem2:2.2.1--he513fc3_0' : 'biocontainers/bwa-mem2:2.2.1--he513fc3_0' }" @@ -18,13 +18,14 @@ process BWAMEM2_INDEX { task.ext.when == null || task.ext.when script: + def prefix = task.ext.prefix ?: "${fasta}" def args = task.ext.args ?: '' """ mkdir bwamem2 bwa-mem2 \\ index \\ $args \\ - $fasta -p bwamem2/${fasta} + $fasta -p bwamem2/${prefix} cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -33,13 +34,15 @@ process BWAMEM2_INDEX { """ stub: + def prefix = task.ext.prefix ?: "${fasta}" + """ mkdir bwamem2 - touch bwamem2/${fasta}.0123 - touch bwamem2/${fasta}.ann - touch bwamem2/${fasta}.pac - touch bwamem2/${fasta}.amb - touch bwamem2/${fasta}.bwt.2bit.64 + touch bwamem2/${prefix}.0123 + touch bwamem2/${prefix}.ann + touch bwamem2/${prefix}.pac + touch bwamem2/${prefix}.amb + touch bwamem2/${prefix}.bwt.2bit.64 cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/bwamem2/index/meta.yml b/modules/nf-core/bwamem2/index/meta.yml index 40c26c3..c14a109 100644 --- a/modules/nf-core/bwamem2/index/meta.yml +++ b/modules/nf-core/bwamem2/index/meta.yml @@ -38,3 +38,5 @@ output: pattern: "versions.yml" authors: - "@maxulysse" +maintainers: + - "@maxulysse" diff --git a/modules/nf-core/bwamem2/index/tests/main.nf.test b/modules/nf-core/bwamem2/index/tests/main.nf.test new file mode 100644 index 0000000..dbf1113 --- /dev/null +++ b/modules/nf-core/bwamem2/index/tests/main.nf.test @@ -0,0 +1,31 @@ +nextflow_process { + + name "Test Process BWAMEM2_INDEX" + tag "modules_nfcore" + tag "modules" + tag "bwamem2" + tag "bwamem2/index" + script "../main.nf" + process "BWAMEM2_INDEX" + + test("BWAMEM2 index") { + + when { + process { + """ + input[0] = [ + [id: 'test'], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } +} diff --git a/modules/nf-core/bwamem2/index/tests/main.nf.test.snap b/modules/nf-core/bwamem2/index/tests/main.nf.test.snap new file mode 100644 index 0000000..69b268e --- /dev/null +++ b/modules/nf-core/bwamem2/index/tests/main.nf.test.snap @@ -0,0 +1,47 @@ +{ + "BWAMEM2 index": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + [ + "genome.fasta.0123:md5,b02870de80106104abcb03cd9463e7d8", + "genome.fasta.amb:md5,3a68b8b2287e07dd3f5f95f4344ba76e", + "genome.fasta.ann:md5,c32e11f6c859f166c7525a9c1d583567", + "genome.fasta.bwt.2bit.64:md5,d097a1b82dee375d41a1ea69895a9216", + "genome.fasta.pac:md5,983e3d2cd6f36e2546e6d25a0da78d66" + ] + ] + ], + "1": [ + "versions.yml:md5,9ffd13d12e7108ed15c58566bc4717d6" + ], + "index": [ + [ + { + "id": "test" + }, + [ + "genome.fasta.0123:md5,b02870de80106104abcb03cd9463e7d8", + "genome.fasta.amb:md5,3a68b8b2287e07dd3f5f95f4344ba76e", + "genome.fasta.ann:md5,c32e11f6c859f166c7525a9c1d583567", + "genome.fasta.bwt.2bit.64:md5,d097a1b82dee375d41a1ea69895a9216", + "genome.fasta.pac:md5,983e3d2cd6f36e2546e6d25a0da78d66" + ] + ] + ], + "versions": [ + "versions.yml:md5,9ffd13d12e7108ed15c58566bc4717d6" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.02.0" + }, + "timestamp": "2024-03-18T12:59:39.132616" + } +} \ No newline at end of file diff --git a/modules/nf-core/bwamem2/index/tests/tags.yml b/modules/nf-core/bwamem2/index/tests/tags.yml new file mode 100644 index 0000000..3953018 --- /dev/null +++ b/modules/nf-core/bwamem2/index/tests/tags.yml @@ -0,0 +1,2 @@ +bwamem2/index: + - modules/nf-core/bwamem2/index/** diff --git a/modules/nf-core/bwamem2/mem/environment.yml b/modules/nf-core/bwamem2/mem/environment.yml new file mode 100644 index 0000000..cbf06d3 --- /dev/null +++ b/modules/nf-core/bwamem2/mem/environment.yml @@ -0,0 +1,10 @@ +name: bwamem2_mem +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bwa-mem2=2.2.1 + # renovate: datasource=conda depName=bioconda/samtools + - samtools=1.19.2 + - htslib=1.19.1 diff --git a/modules/nf-core/bwamem2/mem/main.nf b/modules/nf-core/bwamem2/mem/main.nf index d427dea..729428c 100644 --- a/modules/nf-core/bwamem2/mem/main.nf +++ b/modules/nf-core/bwamem2/mem/main.nf @@ -2,19 +2,24 @@ process BWAMEM2_MEM { tag "$meta.id" label 'process_high' - conda "bioconda::bwa-mem2=2.2.1 bioconda::samtools=1.16.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:2cdf6bf1e92acbeb9b2834b1c58754167173a410-0' : - 'biocontainers/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:2cdf6bf1e92acbeb9b2834b1c58754167173a410-0' }" + 'https://depot.galaxyproject.org/singularity/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:2d15960ccea84e249a150b7f5d4db3a42fc2d6c3-0' : + 'biocontainers/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:2d15960ccea84e249a150b7f5d4db3a42fc2d6c3-0' }" input: tuple val(meta), path(reads) tuple val(meta2), path(index) + tuple val(meta3), path(fasta) val sort_bam output: - tuple val(meta), path("*.bam"), emit: bam - path "versions.yml" , emit: versions + tuple val(meta), path("*.sam") , emit: sam , optional:true + tuple val(meta), path("*.bam") , emit: bam , optional:true + tuple val(meta), path("*.cram") , emit: cram, optional:true + tuple val(meta), path("*.crai") , emit: crai, optional:true + tuple val(meta), path("*.csi") , emit: csi , optional:true + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -24,6 +29,13 @@ process BWAMEM2_MEM { def args2 = task.ext.args2 ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def samtools_command = sort_bam ? 'sort' : 'view' + + def extension_pattern = /(--output-fmt|-O)+\s+(\S+)/ + def extension_matcher = (args2 =~ extension_pattern) + def extension = extension_matcher.getCount() > 0 ? extension_matcher[0][2].toLowerCase() : "bam" + def reference = fasta && extension=="cram" ? "--reference ${fasta}" : "" + if (!fasta && extension=="cram") error "Fasta reference is required for CRAM output" + """ INDEX=`find -L ./ -name "*.amb" | sed 's/\\.amb\$//'` @@ -33,7 +45,7 @@ process BWAMEM2_MEM { -t $task.cpus \\ \$INDEX \\ $reads \\ - | samtools $samtools_command $args2 -@ $task.cpus -o ${prefix}.bam - + | samtools $samtools_command $args2 -@ $task.cpus ${reference} -o ${prefix}.${extension} - cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -43,9 +55,28 @@ process BWAMEM2_MEM { """ stub: + + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + def samtools_command = sort_bam ? 'sort' : 'view' + def extension_pattern = /(--output-fmt|-O)+\s+(\S+)/ + def extension_matcher = (args2 =~ extension_pattern) + def extension = extension_matcher.getCount() > 0 ? extension_matcher[0][2].toLowerCase() : "bam" + def reference = fasta && extension=="cram" ? "--reference ${fasta}" : "" + if (!fasta && extension=="cram") error "Fasta reference is required for CRAM output" + + def create_index = "" + if (extension == "cram") { + create_index = "touch ${prefix}.crai" + } else if (extension == "bam") { + create_index = "touch ${prefix}.csi" + } + """ - touch ${prefix}.bam + touch ${prefix}.${extension} + ${create_index} + cat <<-END_VERSIONS > versions.yml "${task.process}": bwamem2: \$(echo \$(bwa-mem2 version 2>&1) | sed 's/.* //') diff --git a/modules/nf-core/bwamem2/mem/meta.yml b/modules/nf-core/bwamem2/mem/meta.yml index bc3dfcd..931f712 100644 --- a/modules/nf-core/bwamem2/mem/meta.yml +++ b/modules/nf-core/bwamem2/mem/meta.yml @@ -37,6 +37,15 @@ input: type: file description: BWA genome index files pattern: "Directory containing BWA index *.{0132,amb,ann,bwt.2bit.64,pac}" + - meta3: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'genome' ] + - fasta: + type: file + description: Reference genome in FASTA format + pattern: "*.{fa,fasta,fna}" - sort_bam: type: boolean description: use samtools sort (true) or samtools view (false) @@ -47,13 +56,33 @@ output: description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] + - sam: + type: file + description: Output SAM file containing read alignments + pattern: "*.{sam}" - bam: type: file description: Output BAM file containing read alignments pattern: "*.{bam}" + - cram: + type: file + description: Output CRAM file containing read alignments + pattern: "*.{cram}" + - crai: + type: file + description: Index file for CRAM file + pattern: "*.{crai}" + - csi: + type: file + description: Index file for BAM file + pattern: "*.{csi}" - versions: type: file description: File containing software versions pattern: "versions.yml" authors: - "@maxulysse" + - "@matthdsm" +maintainers: + - "@maxulysse" + - "@matthdsm" diff --git a/modules/nf-core/bwamem2/mem/tests/main.nf.test b/modules/nf-core/bwamem2/mem/tests/main.nf.test new file mode 100644 index 0000000..5e67f70 --- /dev/null +++ b/modules/nf-core/bwamem2/mem/tests/main.nf.test @@ -0,0 +1,234 @@ +nextflow_process { + + name "Test Process BWAMEM2_MEM" + script "../main.nf" + process "BWAMEM2_MEM" + + tag "modules" + tag "modules_nfcore" + tag "bwamem2" + tag "bwamem2/mem" + tag "bwamem2/index" + + test("sarscov2 - fastq, index, fasta, false") { + + setup { + run("BWAMEM2_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)] + ]) + input[1] = BWAMEM2_INDEX.out.index + input[2] = Channel.of([[:], [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]]) + input[3] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, index, fasta, true") { + + setup { + run("BWAMEM2_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)] + ]) + input[1] = BWAMEM2_INDEX.out.index + input[2] = Channel.of([[:], [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]]) + input[3] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, fasta, false") { + + setup { + run("BWAMEM2_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = BWAMEM2_INDEX.out.index + input[2] = Channel.of([[:], [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]]) + input[3] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, fasta, true") { + + setup { + run("BWAMEM2_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = BWAMEM2_INDEX.out.index + input[2] = Channel.of([[:], [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]]) + input[3] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, fasta, true - stub") { + + options "-stub" + + setup { + run("BWAMEM2_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = BWAMEM2_INDEX.out.index + input[2] = Channel.of([[:], [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]]) + input[3] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap b/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap new file mode 100644 index 0000000..9fb1e69 --- /dev/null +++ b/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap @@ -0,0 +1,67 @@ +{ + "sarscov2 - [fastq1, fastq2], index, fasta, false": { + "content": [ + "test.bam", + [ + "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-19T13:13:18.890289958" + }, + "sarscov2 - [fastq1, fastq2], index, fasta, true - stub": { + "content": [ + "test.bam", + [ + "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-19T13:45:51.821633029" + }, + "sarscov2 - [fastq1, fastq2], index, fasta, true": { + "content": [ + "test.bam", + [ + "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-19T13:13:36.458291078" + }, + "sarscov2 - fastq, index, fasta, false": { + "content": [ + "test.bam", + [ + "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-19T13:12:44.084654507" + }, + "sarscov2 - fastq, index, fasta, true": { + "content": [ + "test.bam", + [ + "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-19T13:13:01.763341681" + } +} \ No newline at end of file diff --git a/modules/nf-core/bwamem2/mem/tests/tags.yml b/modules/nf-core/bwamem2/mem/tests/tags.yml new file mode 100644 index 0000000..134efb2 --- /dev/null +++ b/modules/nf-core/bwamem2/mem/tests/tags.yml @@ -0,0 +1,2 @@ +bwamem2/mem: + - "modules/nf-core/bwamem2/mem/**" diff --git a/modules/nf-core/crumble/environment.yml b/modules/nf-core/crumble/environment.yml new file mode 100644 index 0000000..de73902 --- /dev/null +++ b/modules/nf-core/crumble/environment.yml @@ -0,0 +1,7 @@ +name: crumble +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::crumble=0.9.1 diff --git a/modules/nf-core/crumble/main.nf b/modules/nf-core/crumble/main.nf index 2699257..70cc180 100644 --- a/modules/nf-core/crumble/main.nf +++ b/modules/nf-core/crumble/main.nf @@ -2,7 +2,7 @@ process CRUMBLE { tag "$meta.id" label 'process_medium' - conda "bioconda::crumble=0.9.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/crumble:0.9.1--hb0d9459_0': 'biocontainers/crumble:0.9.1--hb0d9459_0' }" diff --git a/modules/nf-core/crumble/meta.yml b/modules/nf-core/crumble/meta.yml index cbc0045..59d7a79 100644 --- a/modules/nf-core/crumble/meta.yml +++ b/modules/nf-core/crumble/meta.yml @@ -13,7 +13,6 @@ tools: tool_dev_url: "https://github.com/jkbonfield/crumble" doi: "10.1093/bioinformatics/bty608" licence: "['multiple BSD style licenses']" - input: - meta: type: map @@ -30,7 +29,6 @@ input: - bedout: type: boolean description: set to true to ouput suspicious regions to a BED file - output: - meta: type: map @@ -57,6 +55,7 @@ output: type: file description: optional suspicious regions BED file pattern: "*{bed}" - authors: - "@priyanka-surana" +maintainers: + - "@priyanka-surana" diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml new file mode 100644 index 0000000..b48ced2 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -0,0 +1,7 @@ +name: custom_dumpsoftwareversions +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.20 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index ebc8727..105f926 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : + 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml index c32657d..5f15a5f 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -1,4 +1,4 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: custom_dumpsoftwareversions description: Custom module used to dump software versions within the nf-core pipeline template keywords: @@ -16,7 +16,6 @@ input: type: file description: YML file containing software versions pattern: "*.yml" - output: - yml: type: file @@ -30,7 +29,9 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@drpatelh" - "@grst" +maintainers: + - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py index da03340..9a493ac 100755 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py @@ -3,7 +3,6 @@ """Provide functions to merge multiple versions.yml files.""" - import yaml import platform from textwrap import dedent diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test new file mode 100644 index 0000000..b1e1630 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -0,0 +1,43 @@ +nextflow_process { + + name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" + script "../main.nf" + process "CUSTOM_DUMPSOFTWAREVERSIONS" + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "dumpsoftwareversions" + tag "custom/dumpsoftwareversions" + + test("Should run without failures") { + when { + process { + """ + def tool1_version = ''' + TOOL1: + tool1: 0.11.9 + '''.stripIndent() + + def tool2_version = ''' + TOOL2: + tool2: 1.9 + '''.stripIndent() + + input[0] = Channel.of(tool1_version, tool2_version).collectFile() + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } + ) + } + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap new file mode 100644 index 0000000..5f59a93 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -0,0 +1,33 @@ +{ + "Should run without failures": { + "content": [ + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] + ], + "timestamp": "2024-01-09T23:01:18.710682" + } +} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml new file mode 100644 index 0000000..405aa24 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml @@ -0,0 +1,2 @@ +custom/dumpsoftwareversions: + - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/gunzip/environment.yml b/modules/nf-core/gunzip/environment.yml new file mode 100644 index 0000000..dfc02a7 --- /dev/null +++ b/modules/nf-core/gunzip/environment.yml @@ -0,0 +1,9 @@ +name: gunzip +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - conda-forge::grep=3.11 + - conda-forge::sed=4.8 + - conda-forge::tar=1.34 diff --git a/modules/nf-core/gunzip/main.nf b/modules/nf-core/gunzip/main.nf index e7189d2..5e67e3b 100644 --- a/modules/nf-core/gunzip/main.nf +++ b/modules/nf-core/gunzip/main.nf @@ -2,10 +2,10 @@ process GUNZIP { tag "$archive" label 'process_single' - conda "conda-forge::sed=4.7" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'nf-core/ubuntu:20.04' }" + 'https://depot.galaxyproject.org/singularity/ubuntu:22.04' : + 'nf-core/ubuntu:22.04' }" input: tuple val(meta), path(archive) @@ -18,13 +18,20 @@ process GUNZIP { task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' - gunzip = archive.toString() - '.gz' + def args = task.ext.args ?: '' + def extension = ( archive.toString() - '.gz' ).tokenize('.')[-1] + def name = archive.toString() - '.gz' - ".$extension" + def prefix = task.ext.prefix ?: name + gunzip = prefix + ".$extension" """ - gunzip \\ - -f \\ + # Not calling gunzip itself because it creates files + # with the original group ownership rather than the + # default one for that user / the work directory + gzip \\ + -cd \\ $args \\ - $archive + $archive \\ + > $gunzip cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -33,7 +40,11 @@ process GUNZIP { """ stub: - gunzip = archive.toString() - '.gz' + def args = task.ext.args ?: '' + def extension = ( archive.toString() - '.gz' ).tokenize('.')[-1] + def name = archive.toString() - '.gz' - ".$extension" + def prefix = task.ext.prefix ?: name + gunzip = prefix + ".$extension" """ touch $gunzip cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/gunzip/meta.yml b/modules/nf-core/gunzip/meta.yml index 4cdcdf4..f32973a 100644 --- a/modules/nf-core/gunzip/meta.yml +++ b/modules/nf-core/gunzip/meta.yml @@ -33,3 +33,8 @@ authors: - "@joseespinosa" - "@drpatelh" - "@jfy133" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@jfy133" + - "@gallvp" diff --git a/modules/nf-core/gunzip/tests/main.nf.test b/modules/nf-core/gunzip/tests/main.nf.test new file mode 100644 index 0000000..776211a --- /dev/null +++ b/modules/nf-core/gunzip/tests/main.nf.test @@ -0,0 +1,121 @@ +nextflow_process { + + name "Test Process GUNZIP" + script "../main.nf" + process "GUNZIP" + tag "gunzip" + tag "modules_nfcore" + tag "modules" + + test("Should run without failures") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + ) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Should run without failures - prefix") { + + config './nextflow.config' + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [ id: 'test' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + ) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Should run without failures - stub") { + + options '-stub' + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + ) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Should run without failures - prefix - stub") { + + options '-stub' + config './nextflow.config' + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [ id: 'test' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + ) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/gunzip/tests/main.nf.test.snap b/modules/nf-core/gunzip/tests/main.nf.test.snap new file mode 100644 index 0000000..069967e --- /dev/null +++ b/modules/nf-core/gunzip/tests/main.nf.test.snap @@ -0,0 +1,134 @@ +{ + "Should run without failures - prefix - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.xyz.fastq:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ], + "gunzip": [ + [ + { + "id": "test" + }, + "test.xyz.fastq:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-25T11:35:10.861293" + }, + "Should run without failures - stub": { + "content": [ + { + "0": [ + [ + [ + + ], + "test_1.fastq:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ], + "gunzip": [ + [ + [ + + ], + "test_1.fastq:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-25T11:35:05.857145" + }, + "Should run without failures": { + "content": [ + { + "0": [ + [ + [ + + ], + "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "1": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ], + "gunzip": [ + [ + [ + + ], + "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "versions": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2023-10-17T15:35:37.690477896" + }, + "Should run without failures - prefix": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.xyz.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "1": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ], + "gunzip": [ + [ + { + "id": "test" + }, + "test.xyz.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "versions": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-25T11:33:32.921739" + } +} \ No newline at end of file diff --git a/modules/nf-core/gunzip/tests/nextflow.config b/modules/nf-core/gunzip/tests/nextflow.config new file mode 100644 index 0000000..dec7764 --- /dev/null +++ b/modules/nf-core/gunzip/tests/nextflow.config @@ -0,0 +1,5 @@ +process { + withName: GUNZIP { + ext.prefix = { "${meta.id}.xyz" } + } +} diff --git a/modules/nf-core/gunzip/tests/tags.yml b/modules/nf-core/gunzip/tests/tags.yml new file mode 100644 index 0000000..fd3f691 --- /dev/null +++ b/modules/nf-core/gunzip/tests/tags.yml @@ -0,0 +1,2 @@ +gunzip: + - modules/nf-core/gunzip/** diff --git a/modules/nf-core/minimap2/align/environment.yml b/modules/nf-core/minimap2/align/environment.yml index cf6e775..41e8fe9 100644 --- a/modules/nf-core/minimap2/align/environment.yml +++ b/modules/nf-core/minimap2/align/environment.yml @@ -1,9 +1,11 @@ name: minimap2_align + channels: - conda-forge - bioconda - defaults + dependencies: - - bioconda::minimap2=2.24 - - bioconda::samtools=1.18 - - bioconda::htslib=1.18 + - bioconda::htslib=1.20 + - bioconda::minimap2=2.28 + - bioconda::samtools=1.20 diff --git a/modules/nf-core/minimap2/align/main.nf b/modules/nf-core/minimap2/align/main.nf index 07a3215..66745ef 100644 --- a/modules/nf-core/minimap2/align/main.nf +++ b/modules/nf-core/minimap2/align/main.nf @@ -1,24 +1,26 @@ process MINIMAP2_ALIGN { tag "$meta.id" - label 'process_medium' + label 'process_high' // Note: the versions here need to match the versions used in the mulled container below and minimap2/index conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:365b17b986c1a60c1b82c6066a9345f38317b763-0' : - 'biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:365b17b986c1a60c1b82c6066a9345f38317b763-0' }" + 'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:3161f532a5ea6f1dec9be5667c9efc2afdac6104-0' : + 'biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:3161f532a5ea6f1dec9be5667c9efc2afdac6104-0' }" input: tuple val(meta), path(reads) tuple val(meta2), path(reference) val bam_format + val bam_index_extension val cigar_paf_format val cigar_bam output: - tuple val(meta), path("*.paf"), optional: true, emit: paf - tuple val(meta), path("*.bam"), optional: true, emit: bam - path "versions.yml" , emit: versions + tuple val(meta), path("*.paf") , optional: true, emit: paf + tuple val(meta), path("*.bam") , optional: true, emit: bam + tuple val(meta), path("*.bam.${bam_index_extension}"), optional: true, emit: index + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -27,20 +29,36 @@ process MINIMAP2_ALIGN { def args = task.ext.args ?: '' def args2 = task.ext.args2 ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def bam_output = bam_format ? "-a | samtools sort -@ ${task.cpus} -o ${prefix}.bam ${args2}" : "-o ${prefix}.paf" + def bam_index = bam_index_extension ? "${prefix}.bam##idx##${prefix}.bam.${bam_index_extension} --write-index" : "${prefix}.bam" + def bam_output = bam_format ? "-a | samtools sort -@ ${task.cpus-1} -o ${bam_index} ${args2}" : "-o ${prefix}.paf" def cigar_paf = cigar_paf_format && !bam_format ? "-c" : '' def set_cigar_bam = cigar_bam && bam_format ? "-L" : '' """ minimap2 \\ $args \\ -t $task.cpus \\ - "${reference ?: reads}" \\ - "$reads" \\ + ${reference ?: reads} \\ + $reads \\ $cigar_paf \\ $set_cigar_bam \\ $bam_output + cat <<-END_VERSIONS > versions.yml + "${task.process}": + minimap2: \$(minimap2 --version 2>&1) + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def output_file = bam_format ? "${prefix}.bam" : "${prefix}.paf" + def bam_index = bam_index_extension ? "touch ${prefix}.bam.${bam_index_extension}" : "" + """ + touch $output_file + ${bam_index} + cat <<-END_VERSIONS > versions.yml "${task.process}": minimap2: \$(minimap2 --version 2>&1) diff --git a/modules/nf-core/minimap2/align/meta.yml b/modules/nf-core/minimap2/align/meta.yml index 408522d..8996f88 100644 --- a/modules/nf-core/minimap2/align/meta.yml +++ b/modules/nf-core/minimap2/align/meta.yml @@ -37,6 +37,9 @@ input: - bam_format: type: boolean description: Specify that output should be in BAM format + - bam_index_extension: + type: string + description: BAM alignment index extension (e.g. "bai") - cigar_paf_format: type: boolean description: Specify that output CIGAR should be in PAF format @@ -59,6 +62,10 @@ output: type: file description: Alignment in BAM format pattern: "*.bam" + - index: + type: file + description: BAM alignment index + pattern: "*.bam.*" - versions: type: file description: File containing software versions @@ -68,8 +75,10 @@ authors: - "@sofstam" - "@sateeshperi" - "@jfy133" + - "@fellen31" maintainers: - "@heuermh" - "@sofstam" - "@sateeshperi" - "@jfy133" + - "@fellen31" diff --git a/modules/nf-core/minimap2/align/tests/main.nf.test b/modules/nf-core/minimap2/align/tests/main.nf.test index b634468..c93f436 100644 --- a/modules/nf-core/minimap2/align/tests/main.nf.test +++ b/modules/nf-core/minimap2/align/tests/main.nf.test @@ -9,22 +9,23 @@ nextflow_process { tag "minimap2" tag "minimap2/align" - test("sarscov2 - fastq, fasta, true, false, false") { + test("sarscov2 - fastq, fasta, true, [], false, false") { when { process { """ input[0] = [ [ id:'test', single_end:true ], // meta map - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] input[1] = [ [ id:'test_ref' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] input[2] = true - input[3] = false + input[3] = [] input[4] = false + input[5] = false """ } } @@ -41,6 +42,40 @@ nextflow_process { } + test("sarscov2 - fastq, fasta, true, 'bai', false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = 'bai' + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + file(process.out.index[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + test("sarscov2 - [fastq1, fastq2], fasta, true, false, false") { when { @@ -49,17 +84,18 @@ nextflow_process { input[0] = [ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] ] input[1] = [ [ id:'test_ref' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] input[2] = true - input[3] = false + input[3] = [] input[4] = false + input[5] = false """ } } @@ -83,15 +119,16 @@ nextflow_process { """ input[0] = [ [ id:'test', single_end:true ], // meta map - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), ] input[1] = [ [ id:'test_ref' ], // meta map [] ] input[2] = true - input[3] = false + input[3] = [] input[4] = false + input[5] = false """ } } @@ -108,7 +145,7 @@ nextflow_process { } - test("sarscov2 - fastq, fasta, true, false, false - stub") { + test("sarscov2 - fastq, fasta, true, [], false, false - stub") { options "-stub" @@ -117,15 +154,16 @@ nextflow_process { """ input[0] = [ [ id:'test', single_end:true ], // meta map - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] input[1] = [ [ id:'test_ref' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] input[2] = true - input[3] = false + input[3] = [] input[4] = false + input[5] = false """ } } @@ -133,13 +171,74 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - process.out.versions - ).match() } + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - fastq, fasta, true, 'bai', false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = 'bai' + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - fastq, fasta, false, [], false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = false + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } ) } } -} +} \ No newline at end of file diff --git a/modules/nf-core/minimap2/align/tests/main.nf.test.snap b/modules/nf-core/minimap2/align/tests/main.nf.test.snap index a39a169..ecb3e4e 100644 --- a/modules/nf-core/minimap2/align/tests/main.nf.test.snap +++ b/modules/nf-core/minimap2/align/tests/main.nf.test.snap @@ -1,38 +1,208 @@ { - "sarscov2 - fastq, fasta, true, false, false": { + "sarscov2 - fastq, fasta, true, 'bai', false, false - stub": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ], + "bam": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "index": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "paf": [ + + ], + "versions": [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-03T11:29:44.669021368" + }, + "sarscov2 - fastq, fasta, false, [], false, false - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.paf:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ], + "bam": [ + + ], + "index": [ + + ], + "paf": [ + [ + { + "id": "test", + "single_end": true + }, + "test.paf:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-03T11:15:52.738781039" + }, + "sarscov2 - fastq, fasta, true, [], false, false - stub": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ], + "bam": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "index": [ + + ], + "paf": [ + + ], + "versions": [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-03T11:15:23.033808223" + }, + "sarscov2 - [fastq1, fastq2], fasta, true, false, false": { "content": [ "test.bam", [ - "versions.yml:md5,9e9eeae0002d466d580a9d6e0d003eb1" + "versions.yml:md5,3548eeba9066efbf8d78ea99f8d813fd" ] ], - "timestamp": "2023-12-04T12:07:06.01315354" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-03T12:21:31.582849048" }, - "sarscov2 - fastq, fasta, true, false, false - stub": { + "sarscov2 - fastq, fasta, true, [], false, false": { "content": [ "test.bam", [ - "versions.yml:md5,9e9eeae0002d466d580a9d6e0d003eb1" + "versions.yml:md5,3548eeba9066efbf8d78ea99f8d813fd" ] ], - "timestamp": "2023-12-04T12:07:24.487175659" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-03T12:29:11.968586416" }, - "sarscov2 - [fastq1, fastq2], fasta, true, false, false": { + "sarscov2 - fastq, fasta, true, 'bai', false, false": { "content": [ "test.bam", + "test.bam.bai", [ - "versions.yml:md5,9e9eeae0002d466d580a9d6e0d003eb1" + "versions.yml:md5,3548eeba9066efbf8d78ea99f8d813fd" ] ], - "timestamp": "2023-12-04T12:07:12.50816279" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-03T12:21:20.362186362" }, "sarscov2 - fastq, [], true, false, false": { "content": [ "test.bam", [ - "versions.yml:md5,9e9eeae0002d466d580a9d6e0d003eb1" + "versions.yml:md5,3548eeba9066efbf8d78ea99f8d813fd" ] ], - "timestamp": "2023-12-04T12:07:18.414974788" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-03T12:21:37.233709954" } } \ No newline at end of file diff --git a/modules/nf-core/samtools/faidx/environment.yml b/modules/nf-core/samtools/faidx/environment.yml new file mode 100644 index 0000000..f8450fa --- /dev/null +++ b/modules/nf-core/samtools/faidx/environment.yml @@ -0,0 +1,10 @@ +name: samtools_faidx + +channels: + - conda-forge + - bioconda + - defaults + +dependencies: + - bioconda::htslib=1.20 + - bioconda::samtools=1.20 diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf index 59ed308..bdcdbc9 100644 --- a/modules/nf-core/samtools/faidx/main.nf +++ b/modules/nf-core/samtools/faidx/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_FAIDX { tag "$fasta" label 'process_single' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : + 'biocontainers/samtools:1.20--h50ea8bc_0' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml index 957b25e..f3c25de 100644 --- a/modules/nf-core/samtools/faidx/meta.yml +++ b/modules/nf-core/samtools/faidx/meta.yml @@ -39,6 +39,10 @@ output: description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] + - fa: + type: file + description: FASTA file + pattern: "*.{fa}" - fai: type: file description: FASTA index file @@ -55,3 +59,7 @@ authors: - "@drpatelh" - "@ewels" - "@phue" +maintainers: + - "@drpatelh" + - "@ewels" + - "@phue" diff --git a/modules/nf-core/samtools/faidx/tests/main.nf.test b/modules/nf-core/samtools/faidx/tests/main.nf.test new file mode 100644 index 0000000..17244ef --- /dev/null +++ b/modules/nf-core/samtools/faidx/tests/main.nf.test @@ -0,0 +1,122 @@ +nextflow_process { + + name "Test Process SAMTOOLS_FAIDX" + script "../main.nf" + process "SAMTOOLS_FAIDX" + + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/faidx" + + test("test_samtools_faidx") { + + when { + process { + """ + input[0] = [ [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] + + input[1] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("test_samtools_faidx_bgzip") { + + when { + process { + """ + input[0] = [ [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true)] + + input[1] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("test_samtools_faidx_fasta") { + + config "./nextflow.config" + + when { + process { + """ + input[0] = [ [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] + + input[1] = [ [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("test_samtools_faidx_stub_fasta") { + + config "./nextflow2.config" + + when { + process { + """ + input[0] = [ [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] + + input[1] = [ [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("test_samtools_faidx_stub_fai") { + + when { + process { + """ + input[0] = [ [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] + + input[1] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/faidx/tests/main.nf.test.snap b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap new file mode 100644 index 0000000..3223b72 --- /dev/null +++ b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap @@ -0,0 +1,249 @@ +{ + "test_samtools_faidx": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5" + ] + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,2db78952923a61e05d50b95518b21856" + ], + "fa": [ + + ], + "fai": [ + [ + { + "id": "test", + "single_end": false + }, + "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5" + ] + ], + "gzi": [ + + ], + "versions": [ + "versions.yml:md5,2db78952923a61e05d50b95518b21856" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:42:14.779784761" + }, + "test_samtools_faidx_bgzip": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5" + ] + ], + "2": [ + [ + { + "id": "test", + "single_end": false + }, + "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474" + ] + ], + "3": [ + "versions.yml:md5,2db78952923a61e05d50b95518b21856" + ], + "fa": [ + + ], + "fai": [ + [ + { + "id": "test", + "single_end": false + }, + "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5" + ] + ], + "gzi": [ + [ + { + "id": "test", + "single_end": false + }, + "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474" + ] + ], + "versions": [ + "versions.yml:md5,2db78952923a61e05d50b95518b21856" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:42:20.256633877" + }, + "test_samtools_faidx_fasta": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "extract.fa:md5,6a0774a0ad937ba0bfd2ac7457d90f36" + ] + ], + "1": [ + + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,2db78952923a61e05d50b95518b21856" + ], + "fa": [ + [ + { + "id": "test", + "single_end": false + }, + "extract.fa:md5,6a0774a0ad937ba0bfd2ac7457d90f36" + ] + ], + "fai": [ + + ], + "gzi": [ + + ], + "versions": [ + "versions.yml:md5,2db78952923a61e05d50b95518b21856" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:42:25.632577273" + }, + "test_samtools_faidx_stub_fasta": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "extract.fa:md5,9da2a56e2853dc8c0b86a9e7229c9fe5" + ] + ], + "1": [ + + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,2db78952923a61e05d50b95518b21856" + ], + "fa": [ + [ + { + "id": "test", + "single_end": false + }, + "extract.fa:md5,9da2a56e2853dc8c0b86a9e7229c9fe5" + ] + ], + "fai": [ + + ], + "gzi": [ + + ], + "versions": [ + "versions.yml:md5,2db78952923a61e05d50b95518b21856" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:42:31.058424849" + }, + "test_samtools_faidx_stub_fai": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5" + ] + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,2db78952923a61e05d50b95518b21856" + ], + "fa": [ + + ], + "fai": [ + [ + { + "id": "test", + "single_end": false + }, + "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5" + ] + ], + "gzi": [ + + ], + "versions": [ + "versions.yml:md5,2db78952923a61e05d50b95518b21856" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:42:36.479929617" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/faidx/tests/nextflow.config b/modules/nf-core/samtools/faidx/tests/nextflow.config new file mode 100644 index 0000000..f76a3ba --- /dev/null +++ b/modules/nf-core/samtools/faidx/tests/nextflow.config @@ -0,0 +1,7 @@ +process { + + withName: SAMTOOLS_FAIDX { + ext.args = 'MT192765.1 -o extract.fa' + } + +} diff --git a/modules/nf-core/samtools/faidx/tests/nextflow2.config b/modules/nf-core/samtools/faidx/tests/nextflow2.config new file mode 100644 index 0000000..33ebbd5 --- /dev/null +++ b/modules/nf-core/samtools/faidx/tests/nextflow2.config @@ -0,0 +1,6 @@ +process { + + withName: SAMTOOLS_FAIDX { + ext.args = '-o extract.fa' + } +} diff --git a/modules/nf-core/samtools/faidx/tests/tags.yml b/modules/nf-core/samtools/faidx/tests/tags.yml new file mode 100644 index 0000000..e4a8394 --- /dev/null +++ b/modules/nf-core/samtools/faidx/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/faidx: + - modules/nf-core/samtools/faidx/** diff --git a/modules/nf-core/samtools/fastq/environment.yml b/modules/nf-core/samtools/fastq/environment.yml new file mode 100644 index 0000000..4455904 --- /dev/null +++ b/modules/nf-core/samtools/fastq/environment.yml @@ -0,0 +1,8 @@ +name: samtools_fastq +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.20 + - bioconda::htslib=1.20 diff --git a/modules/nf-core/samtools/fastq/main.nf b/modules/nf-core/samtools/fastq/main.nf index 15d8976..6796c02 100644 --- a/modules/nf-core/samtools/fastq/main.nf +++ b/modules/nf-core/samtools/fastq/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_FASTQ { tag "$meta.id" label 'process_low' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : + 'biocontainers/samtools:1.20--h50ea8bc_0' }" input: tuple val(meta), path(input) @@ -13,7 +13,7 @@ process SAMTOOLS_FASTQ { output: tuple val(meta), path("*_{1,2}.fastq.gz") , optional:true, emit: fastq - tuple val(meta), path("*_interleaved.fastq.gz"), optional:true, emit: interleaved + tuple val(meta), path("*_interleaved.fastq") , optional:true, emit: interleaved tuple val(meta), path("*_singleton.fastq.gz") , optional:true, emit: singleton tuple val(meta), path("*_other.fastq.gz") , optional:true, emit: other path "versions.yml" , emit: versions @@ -24,7 +24,7 @@ process SAMTOOLS_FASTQ { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def output = ( interleave && ! meta.single_end ) ? "> ${prefix}_interleaved.fastq.gz" : + def output = ( interleave && ! meta.single_end ) ? "> ${prefix}_interleaved.fastq" : meta.single_end ? "-1 ${prefix}_1.fastq.gz -s ${prefix}_singleton.fastq.gz" : "-1 ${prefix}_1.fastq.gz -2 ${prefix}_2.fastq.gz -s ${prefix}_singleton.fastq.gz" """ diff --git a/modules/nf-core/samtools/fastq/meta.yml b/modules/nf-core/samtools/fastq/meta.yml index b1a1ed3..c4002a4 100644 --- a/modules/nf-core/samtools/fastq/meta.yml +++ b/modules/nf-core/samtools/fastq/meta.yml @@ -15,7 +15,6 @@ tools: documentation: http://www.htslib.org/doc/samtools.html doi: 10.1093/bioinformatics/btp352 licence: ["MIT"] - input: - meta: type: map @@ -29,7 +28,6 @@ input: - interleave: type: boolean description: Set true for interleaved fastq file - output: - meta: type: map @@ -56,7 +54,9 @@ output: type: file description: Compressed FASTQ file with reads with either both READ1 and READ2 flags set or unset pattern: "*_other.fastq.gz" - authors: - "@priyanka-surana" - "@suzannejin" +maintainers: + - "@priyanka-surana" + - "@suzannejin" diff --git a/modules/nf-core/samtools/fastq/tests/main.nf.test b/modules/nf-core/samtools/fastq/tests/main.nf.test new file mode 100644 index 0000000..f6ac112 --- /dev/null +++ b/modules/nf-core/samtools/fastq/tests/main.nf.test @@ -0,0 +1,67 @@ +nextflow_process { + + name "Test Process SAMTOOLS_FASTQ" + script "../main.nf" + process "SAMTOOLS_FASTQ" + + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/fastq" + + test("bam") { + + when { + process { + """ + interleave = false + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true) + ]) + input[1] = interleave + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.fastq[0][1].collect { path(it).linesGzip[0..6] }).match("bam_fastq") }, + { assert snapshot(process.out.interleaved).match("bam_interleaved") }, + { assert snapshot(file(process.out.singleton[0][1]).name).match("bam_singleton") }, + { assert snapshot(file(process.out.other[0][1]).name).match("bam_other") }, + { assert snapshot(process.out.versions).match("bam_versions") } + ) + } + } + + test("bam_interleave") { + + when { + process { + """ + interleave = true + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true) + ]) + input[1] = interleave + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.fastq).match("bam_interleave_fastq") }, + { assert snapshot(path(process.out.interleaved[0][1]).readLines()[0..6]).match("bam_interlinterleave_eaved") }, + { assert snapshot(process.out.singleton).match("bam_singinterleave_leton") }, + { assert snapshot(file(process.out.other[0][1]).name).match("bam_interleave_other") }, + { assert snapshot(process.out.versions).match("bam_verinterleave_sions") } + ) + } + } +} diff --git a/modules/nf-core/samtools/fastq/tests/main.nf.test.snap b/modules/nf-core/samtools/fastq/tests/main.nf.test.snap new file mode 100644 index 0000000..1ba09d3 --- /dev/null +++ b/modules/nf-core/samtools/fastq/tests/main.nf.test.snap @@ -0,0 +1,139 @@ +{ + "bam_interlinterleave_eaved": { + "content": [ + [ + "@ERR5069949.2151832/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "+", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.flagstat + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/samtools/flagstat/meta.yml b/modules/nf-core/samtools/flagstat/meta.yml index 954225d..9799135 100644 --- a/modules/nf-core/samtools/flagstat/meta.yml +++ b/modules/nf-core/samtools/flagstat/meta.yml @@ -47,3 +47,5 @@ output: pattern: "versions.yml" authors: - "@drpatelh" +maintainers: + - "@drpatelh" diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test b/modules/nf-core/samtools/flagstat/tests/main.nf.test new file mode 100644 index 0000000..24c3c04 --- /dev/null +++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test @@ -0,0 +1,36 @@ +nextflow_process { + + name "Test Process SAMTOOLS_FLAGSTAT" + script "../main.nf" + process "SAMTOOLS_FLAGSTAT" + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/flagstat" + + test("BAM") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.flagstat).match("flagstat") }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } +} diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap new file mode 100644 index 0000000..e9f85ef --- /dev/null +++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap @@ -0,0 +1,32 @@ +{ + "flagstat": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:31:37.783927" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,f606681ef971cbb548a4d9e3fbabdbc2" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:41:52.516253882" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/flagstat/tests/tags.yml b/modules/nf-core/samtools/flagstat/tests/tags.yml new file mode 100644 index 0000000..2d2b725 --- /dev/null +++ b/modules/nf-core/samtools/flagstat/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/flagstat: + - modules/nf-core/samtools/flagstat/** diff --git a/modules/nf-core/samtools/idxstats/environment.yml b/modules/nf-core/samtools/idxstats/environment.yml new file mode 100644 index 0000000..eb6c880 --- /dev/null +++ b/modules/nf-core/samtools/idxstats/environment.yml @@ -0,0 +1,8 @@ +name: samtools_idxstats +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.20 + - bioconda::htslib=1.20 diff --git a/modules/nf-core/samtools/idxstats/main.nf b/modules/nf-core/samtools/idxstats/main.nf index a257d70..2ea2a5c 100644 --- a/modules/nf-core/samtools/idxstats/main.nf +++ b/modules/nf-core/samtools/idxstats/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_IDXSTATS { tag "$meta.id" label 'process_single' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : + 'biocontainers/samtools:1.20--h50ea8bc_0' }" input: tuple val(meta), path(bam), path(bai) @@ -33,4 +33,16 @@ process SAMTOOLS_IDXSTATS { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + touch ${prefix}.idxstats + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/samtools/idxstats/meta.yml b/modules/nf-core/samtools/idxstats/meta.yml index dda87e1..344e92a 100644 --- a/modules/nf-core/samtools/idxstats/meta.yml +++ b/modules/nf-core/samtools/idxstats/meta.yml @@ -48,3 +48,5 @@ output: pattern: "versions.yml" authors: - "@drpatelh" +maintainers: + - "@drpatelh" diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test b/modules/nf-core/samtools/idxstats/tests/main.nf.test new file mode 100644 index 0000000..a2dcb27 --- /dev/null +++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test @@ -0,0 +1,36 @@ +nextflow_process { + + name "Test Process SAMTOOLS_IDXSTATS" + script "../main.nf" + process "SAMTOOLS_IDXSTATS" + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/idxstats" + + test("bam") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.idxstats).match("idxstats") }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } +} diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap new file mode 100644 index 0000000..4eacdb9 --- /dev/null +++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap @@ -0,0 +1,32 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,7acbcb2a8ec6436ba7b2916d3ff13351" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:46:46.617989517" + }, + "idxstats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:36:41.561026" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/idxstats/tests/tags.yml b/modules/nf-core/samtools/idxstats/tests/tags.yml new file mode 100644 index 0000000..d3057c6 --- /dev/null +++ b/modules/nf-core/samtools/idxstats/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/idxstats: + - modules/nf-core/samtools/idxstats/** diff --git a/modules/nf-core/samtools/merge/environment.yml b/modules/nf-core/samtools/merge/environment.yml new file mode 100644 index 0000000..cd366d6 --- /dev/null +++ b/modules/nf-core/samtools/merge/environment.yml @@ -0,0 +1,8 @@ +name: samtools_merge +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.20 + - bioconda::htslib=1.20 diff --git a/modules/nf-core/samtools/merge/main.nf b/modules/nf-core/samtools/merge/main.nf index 5fb8ede..170f135 100644 --- a/modules/nf-core/samtools/merge/main.nf +++ b/modules/nf-core/samtools/merge/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_MERGE { tag "$meta.id" label 'process_low' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : + 'biocontainers/samtools:1.20--h50ea8bc_0' }" input: tuple val(meta), path(input_files, stageAs: "?/*") @@ -16,6 +16,7 @@ process SAMTOOLS_MERGE { tuple val(meta), path("${prefix}.bam") , optional:true, emit: bam tuple val(meta), path("${prefix}.cram"), optional:true, emit: cram tuple val(meta), path("*.csi") , optional:true, emit: csi + tuple val(meta), path("*.crai") , optional:true, emit: crai path "versions.yml" , emit: versions @@ -48,10 +49,14 @@ process SAMTOOLS_MERGE { """ stub: + def args = task.ext.args ?: '' prefix = task.ext.suffix ? "${meta.id}${task.ext.suffix}" : "${meta.id}" def file_type = input_files instanceof List ? input_files[0].getExtension() : input_files.getExtension() + def index_type = file_type == "bam" ? "csi" : "crai" + def index = args.contains("--write-index") ? "touch ${prefix}.${index_type}" : "" """ touch ${prefix}.${file_type} + ${index} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/samtools/merge/meta.yml b/modules/nf-core/samtools/merge/meta.yml index 3a815f7..2e8f3db 100644 --- a/modules/nf-core/samtools/merge/meta.yml +++ b/modules/nf-core/samtools/merge/meta.yml @@ -65,9 +65,19 @@ output: type: file description: BAM index file (optional) pattern: "*.csi" + - crai: + type: file + description: CRAM index file (optional) + pattern: "*.crai" authors: - "@drpatelh" - "@yuukiiwa " - "@maxulysse" - "@FriederikeHanssen" - "@ramprasadn" +maintainers: + - "@drpatelh" + - "@yuukiiwa " + - "@maxulysse" + - "@FriederikeHanssen" + - "@ramprasadn" diff --git a/modules/nf-core/samtools/merge/samtools-merge.diff b/modules/nf-core/samtools/merge/samtools-merge.diff index cca0b3c..ac3c5ee 100644 --- a/modules/nf-core/samtools/merge/samtools-merge.diff +++ b/modules/nf-core/samtools/merge/samtools-merge.diff @@ -1,7 +1,7 @@ Changes in module 'nf-core/samtools/merge' --- modules/nf-core/samtools/merge/main.nf +++ modules/nf-core/samtools/merge/main.nf -@@ -26,6 +26,11 @@ +@@ -27,6 +27,11 @@ def args = task.ext.args ?: '' prefix = task.ext.prefix ?: "${meta.id}" def file_type = input_files instanceof List ? input_files[0].getExtension() : input_files.getExtension() @@ -13,7 +13,7 @@ Changes in module 'nf-core/samtools/merge' def reference = fasta ? "--reference ${fasta}" : "" """ samtools \\ -@@ -34,7 +39,7 @@ +@@ -35,7 +40,7 @@ $args \\ ${reference} \\ ${prefix}.${file_type} \\ diff --git a/modules/nf-core/samtools/merge/tests/index.config b/modules/nf-core/samtools/merge/tests/index.config new file mode 100644 index 0000000..8c5668c --- /dev/null +++ b/modules/nf-core/samtools/merge/tests/index.config @@ -0,0 +1,3 @@ +process { + ext.args = "--write-index" +} \ No newline at end of file diff --git a/modules/nf-core/samtools/merge/tests/main.nf.test b/modules/nf-core/samtools/merge/tests/main.nf.test new file mode 100644 index 0000000..40b36e8 --- /dev/null +++ b/modules/nf-core/samtools/merge/tests/main.nf.test @@ -0,0 +1,137 @@ +nextflow_process { + + name "Test Process SAMTOOLS_MERGE" + script "../main.nf" + process "SAMTOOLS_MERGE" + + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/merge" + + test("bams") { + + config "./index.config" + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.methylated.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam', checkIfExists: true) ] + ]) + input[1] = [[],[]] + input[2] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.bam[0][1]).name).match("bams_bam") }, + { assert snapshot(process.out.cram).match("bams_cram") }, + { assert snapshot(file(process.out.csi[0][1]).name).match("bams_csi") }, + { assert snapshot(process.out.crai).match("bams_crai") }, + { assert snapshot(process.out.versions).match("bams_versions") } + ) + } + } + + test("crams") { + + config "./index.config" + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test2.paired_end.recalibrated.sorted.cram', checkIfExists: true) ] + ]) + input[1] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.cram[0][1]).name).match("crams_cram") }, + { assert snapshot(process.out.bam).match("crams_bam") }, + { assert snapshot(file(process.out.crai[0][1]).name).match("crams_crai") }, + { assert snapshot(process.out.csi).match("crams_csi") }, + { assert snapshot(process.out.versions).match("crams_versions") } + ) + } + } + + test("bam") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.methylated.sorted.bam', checkIfExists: true) ] + ]) + input[1] = [[],[]] + input[2] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.bam[0][1]).name).match("bam_bam") }, + { assert snapshot(process.out.cram).match("bam_cram") }, + { assert snapshot(process.out.crai).match("bam_crai") }, + { assert snapshot(process.out.csi).match("bam_csi") }, + { assert snapshot(process.out.versions).match("bam_versions") } + ) + } + } + + test("bams_stub") { + + config "./index.config" + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.methylated.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam', checkIfExists: true) ] + ]) + input[1] = [[],[]] + input[2] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.bam[0][1]).name).match("bams_stub_bam") }, + { assert snapshot(process.out.cram).match("bams_stub_cram") }, + { assert snapshot(file(process.out.csi[0][1]).name).match("bams_stub_csi") }, + { assert snapshot(process.out.crai).match("bams_stub_crai") }, + { assert snapshot(process.out.versions).match("bams_stub_versions") } + ) + } + } +} diff --git a/modules/nf-core/samtools/merge/tests/main.nf.test.snap b/modules/nf-core/samtools/merge/tests/main.nf.test.snap new file mode 100644 index 0000000..17bc846 --- /dev/null +++ b/modules/nf-core/samtools/merge/tests/main.nf.test.snap @@ -0,0 +1,228 @@ +{ + "crams_cram": { + "content": [ + "test.cram" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:00.647389" + }, + "bams_stub_cram": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:19.937013" + }, + "bams_crai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:49:24.928616" + }, + "bams_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:49:24.923289" + }, + "bams_cram": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:49:24.925716" + }, + "crams_csi": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:00.655959" + }, + "bam_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:10.319539" + }, + "bam_versions": { + "content": [ + [ + "versions.yml:md5,84dab54b9812780df48f5cecef690c34" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:46:35.851936597" + }, + "bams_csi": { + "content": [ + "test.bam.csi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:49:24.92719" + }, + "bams_stub_csi": { + "content": [ + "test.csi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:19.940498" + }, + "bam_crai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:10.328852" + }, + "bams_stub_versions": { + "content": [ + [ + "versions.yml:md5,84dab54b9812780df48f5cecef690c34" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:46:41.405707643" + }, + "bam_cram": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:10.324219" + }, + "bams_stub_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:19.933153" + }, + "bams_versions": { + "content": [ + [ + "versions.yml:md5,84dab54b9812780df48f5cecef690c34" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:45:51.695689923" + }, + "crams_bam": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:00.650652" + }, + "crams_versions": { + "content": [ + [ + "versions.yml:md5,84dab54b9812780df48f5cecef690c34" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:46:30.185392319" + }, + "bam_csi": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:10.33292" + }, + "crams_crai": { + "content": [ + "test.cram.crai" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:00.653512" + }, + "bams_stub_crai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:19.943839" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/merge/tests/tags.yml b/modules/nf-core/samtools/merge/tests/tags.yml new file mode 100644 index 0000000..b869abc --- /dev/null +++ b/modules/nf-core/samtools/merge/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/merge: + - "modules/nf-core/samtools/merge/**" diff --git a/modules/nf-core/samtools/stats/environment.yml b/modules/nf-core/samtools/stats/environment.yml new file mode 100644 index 0000000..1cc83bd --- /dev/null +++ b/modules/nf-core/samtools/stats/environment.yml @@ -0,0 +1,8 @@ +name: samtools_stats +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.20 + - bioconda::htslib=1.20 diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf index 4a2607d..982bc28 100644 --- a/modules/nf-core/samtools/stats/main.nf +++ b/modules/nf-core/samtools/stats/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_STATS { tag "$meta.id" label 'process_single' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : + 'biocontainers/samtools:1.20--h50ea8bc_0' }" input: tuple val(meta), path(input), path(input_index) diff --git a/modules/nf-core/samtools/stats/meta.yml b/modules/nf-core/samtools/stats/meta.yml index 90e6345..735ff81 100644 --- a/modules/nf-core/samtools/stats/meta.yml +++ b/modules/nf-core/samtools/stats/meta.yml @@ -57,3 +57,7 @@ authors: - "@drpatelh" - "@FriederikeHanssen" - "@ramprasadn" +maintainers: + - "@drpatelh" + - "@FriederikeHanssen" + - "@ramprasadn" diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test b/modules/nf-core/samtools/stats/tests/main.nf.test new file mode 100644 index 0000000..e3d5cb1 --- /dev/null +++ b/modules/nf-core/samtools/stats/tests/main.nf.test @@ -0,0 +1,65 @@ +nextflow_process { + + name "Test Process SAMTOOLS_STATS" + script "../main.nf" + process "SAMTOOLS_STATS" + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/stats" + + test("bam") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true) + ]) + input[1] = [[],[]] + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert snapshot(process.out).match()} + ) + } + } + + test("cram") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert snapshot(process.out).match()} + ) + } + } +} diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test.snap b/modules/nf-core/samtools/stats/tests/main.nf.test.snap new file mode 100644 index 0000000..2747fd6 --- /dev/null +++ b/modules/nf-core/samtools/stats/tests/main.nf.test.snap @@ -0,0 +1,72 @@ +{ + "cram": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,c9d39b38c22de2057fc2f89949090975" + ] + ], + "1": [ + "versions.yml:md5,b3b70b126f867fdbb7dcea5e36e49d4a" + ], + "stats": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,c9d39b38c22de2057fc2f89949090975" + ] + ], + "versions": [ + "versions.yml:md5,b3b70b126f867fdbb7dcea5e36e49d4a" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:45:24.403941966" + }, + "bam": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,d522a1fa016b259d6a55620ae53dcd63" + ] + ], + "1": [ + "versions.yml:md5,b3b70b126f867fdbb7dcea5e36e49d4a" + ], + "stats": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,d522a1fa016b259d6a55620ae53dcd63" + ] + ], + "versions": [ + "versions.yml:md5,b3b70b126f867fdbb7dcea5e36e49d4a" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:45:06.711251947" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/stats/tests/tags.yml b/modules/nf-core/samtools/stats/tests/tags.yml new file mode 100644 index 0000000..7c28e30 --- /dev/null +++ b/modules/nf-core/samtools/stats/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/stats: + - modules/nf-core/samtools/stats/** diff --git a/modules/nf-core/samtools/view/environment.yml b/modules/nf-core/samtools/view/environment.yml new file mode 100644 index 0000000..150c377 --- /dev/null +++ b/modules/nf-core/samtools/view/environment.yml @@ -0,0 +1,8 @@ +name: samtools_view +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.20 + - bioconda::htslib=1.20 diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf index cb91fac..dc61144 100644 --- a/modules/nf-core/samtools/view/main.nf +++ b/modules/nf-core/samtools/view/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_VIEW { tag "$meta.id" label 'process_low' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : + 'biocontainers/samtools:1.20--h50ea8bc_0' }" input: tuple val(meta), path(input), path(index) @@ -13,13 +13,15 @@ process SAMTOOLS_VIEW { path qname output: - tuple val(meta), path("*.bam"), emit: bam, optional: true - tuple val(meta), path("*.cram"), emit: cram, optional: true - tuple val(meta), path("*.sam"), emit: sam, optional: true - tuple val(meta), path("*.bai"), emit: bai, optional: true - tuple val(meta), path("*.csi"), emit: csi, optional: true - tuple val(meta), path("*.crai"), emit: crai, optional: true - path "versions.yml", emit: versions + tuple val(meta), path("${prefix}.bam"), emit: bam, optional: true + tuple val(meta), path("${prefix}.cram"), emit: cram, optional: true + tuple val(meta), path("${prefix}.sam"), emit: sam, optional: true + tuple val(meta), path("${prefix}.${file_type}.bai"), emit: bai, optional: true + tuple val(meta), path("${prefix}.${file_type}.csi"), emit: csi, optional: true + tuple val(meta), path("${prefix}.${file_type}.crai"), emit: crai, optional: true + tuple val(meta), path("${prefix}.unselected.${file_type}"), emit: unselected, optional: true + tuple val(meta), path("${prefix}.unselected.${file_type}.{bai,csi,crsi}"), emit: unselected_index, optional: true + path "versions.yml", emit: versions when: task.ext.when == null || task.ext.when @@ -27,13 +29,13 @@ process SAMTOOLS_VIEW { script: def args = task.ext.args ?: '' def args2 = task.ext.args2 ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" + prefix = task.ext.prefix ?: "${meta.id}" def reference = fasta ? "--reference ${fasta}" : "" - def readnames = qname ? "--qname-file ${qname}": "" - def file_type = args.contains("--output-fmt sam") ? "sam" : - args.contains("--output-fmt bam") ? "bam" : - args.contains("--output-fmt cram") ? "cram" : - input.getExtension() + file_type = args.contains("--output-fmt sam") ? "sam" : + args.contains("--output-fmt bam") ? "bam" : + args.contains("--output-fmt cram") ? "cram" : + input.getExtension() + readnames = qname ? "--qname-file ${qname} --output-unselected ${prefix}.unselected.${file_type}": "" if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" """ samtools \\ @@ -53,10 +55,19 @@ process SAMTOOLS_VIEW { """ stub: - def prefix = task.ext.prefix ?: "${meta.id}" + def args = task.ext.args ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + file_type = args.contains("--output-fmt sam") ? "sam" : + args.contains("--output-fmt bam") ? "bam" : + args.contains("--output-fmt cram") ? "cram" : + input.getExtension() + if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + + def index = args.contains("--write-index") ? "touch ${prefix}.${file_type}.csi" : "" + """ - touch ${prefix}.bam - touch ${prefix}.cram + touch ${prefix}.${file_type} + ${index} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/samtools/view/meta.yml b/modules/nf-core/samtools/view/meta.yml index 3b05450..27be60d 100644 --- a/modules/nf-core/samtools/view/meta.yml +++ b/modules/nf-core/samtools/view/meta.yml @@ -73,6 +73,15 @@ output: type: file description: optional CRAM file index pattern: "*.{crai}" + # unselected and unselected_index are created when passing a qname + - unselected: + type: file + description: optional file with unselected alignments + pattern: "*.unselected.{bam,cram,sam}" + - unselected_index: + type: file + description: index for the "unselected" file + pattern: "*.unselected.{bai,csi,crai}" - versions: type: file description: File containing software versions @@ -82,3 +91,8 @@ authors: - "@joseespinosa" - "@FriederikeHanssen" - "@priyanka-surana" +maintainers: + - "@drpatelh" + - "@joseespinosa" + - "@FriederikeHanssen" + - "@priyanka-surana" diff --git a/modules/nf-core/samtools/view/tests/bam.config b/modules/nf-core/samtools/view/tests/bam.config new file mode 100644 index 0000000..c10d108 --- /dev/null +++ b/modules/nf-core/samtools/view/tests/bam.config @@ -0,0 +1,3 @@ +process { + ext.args = "--output-fmt bam" +} \ No newline at end of file diff --git a/modules/nf-core/samtools/view/tests/bam_index.config b/modules/nf-core/samtools/view/tests/bam_index.config new file mode 100644 index 0000000..771ae03 --- /dev/null +++ b/modules/nf-core/samtools/view/tests/bam_index.config @@ -0,0 +1,3 @@ +process { + ext.args = "--output-fmt bam --write-index" +} \ No newline at end of file diff --git a/modules/nf-core/samtools/view/tests/main.nf.test b/modules/nf-core/samtools/view/tests/main.nf.test new file mode 100644 index 0000000..37b81a9 --- /dev/null +++ b/modules/nf-core/samtools/view/tests/main.nf.test @@ -0,0 +1,214 @@ +nextflow_process { + + name "Test Process SAMTOOLS_VIEW" + script "../main.nf" + process "SAMTOOLS_VIEW" + + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/view" + + test("bam") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true), + [] + ]) + input[1] = [[],[]] + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.bam[0][1]).name).match("bam_bam") }, + { assert snapshot(process.out.bai).match("bam_bai") }, + { assert snapshot(process.out.crai).match("bam_crai") }, + { assert snapshot(process.out.cram).match("bam_cram") }, + { assert snapshot(process.out.csi).match("bam_csi") }, + { assert snapshot(process.out.sam).match("bam_sam") }, + { assert snapshot(process.out.versions).match("bam_versions") } + ) + } + } + + test("cram") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.cram[0][1]).name).match("cram_cram") }, + { assert snapshot(process.out.bai).match("cram_bai") }, + { assert snapshot(process.out.bam).match("cram_bam") }, + { assert snapshot(process.out.crai).match("cram_crai") }, + { assert snapshot(process.out.csi).match("cram_csi") }, + { assert snapshot(process.out.sam).match("cram_sam") }, + { assert snapshot(process.out.versions).match("cram_versions") } + ) + } + } + + test("cram_to_bam") { + + config "./bam.config" + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true), + [] + ]) + input[1] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_bam") }, + { assert snapshot(process.out.bai).match("cram_to_bam_bai") }, + { assert snapshot(process.out.crai).match("cram_to_bam_crai") }, + { assert snapshot(process.out.cram).match("cram_to_bam_cram") }, + { assert snapshot(process.out.csi).match("cram_to_bam_csi") }, + { assert snapshot(process.out.sam).match("cram_to_bam_sam") }, + { assert snapshot(process.out.versions).match("cram_to_bam_versions") } + ) + } + } + + test("cram_to_bam_index") { + + config "./bam_index.config" + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true), + [] + ]) + input[1] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_index_bam") }, + { assert snapshot(file(process.out.csi[0][1]).name).match("cram_to_bam_index_csi") }, + { assert snapshot(process.out.bai).match("cram_to_bam_index_bai") }, + { assert snapshot(process.out.crai).match("cram_to_bam_index_crai") }, + { assert snapshot(process.out.cram).match("cram_to_bam_index_cram") }, + { assert snapshot(process.out.sam).match("cram_to_bam_index_sam") }, + { assert snapshot(process.out.versions).match("cram_to_bam_index_versions") } + ) + } + } + + test("cram_to_bam_index_qname") { + + config "./bam_index.config" + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true), + [] + ]) + input[1] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of("testN:2817", "testN:2814").collectFile(name: "readnames.list", newLine: true) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_index_qname_bam") }, + { assert snapshot(file(process.out.csi[0][1]).name).match("cram_to_bam_index_qname_csi") }, + { assert snapshot(process.out.bai).match("cram_to_bam_index_qname_bai") }, + { assert snapshot(process.out.crai).match("cram_to_bam_index_qname_crai") }, + { assert snapshot(process.out.cram).match("cram_to_bam_index_qname_cram") }, + { assert snapshot(process.out.sam).match("cram_to_bam_index_qname_sam") }, + { assert snapshot(file(process.out.unselected[0][1]).name).match("cram_to_bam_index_qname_unselected") }, + { assert snapshot(file(process.out.unselected_index[0][1]).name).match("cram_to_bam_index_qname_unselected_csi") }, + { assert snapshot(process.out.versions).match("cram_to_bam_index_qname_versions") } + ) + } + } + + test("bam_stub") { + + options "-stub" + config "./bam_index.config" + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true), + [] + ]) + input[1] = [[],[]] + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.bam[0][1]).name).match("bam_stub_bam") }, + { assert snapshot(file(process.out.csi[0][1]).name).match("bam_stub_csi") }, + { assert snapshot(process.out.bai).match("bam_stub_bai") }, + { assert snapshot(process.out.crai).match("bam_stub_crai") }, + { assert snapshot(process.out.cram).match("bam_stub_cram") }, + { assert snapshot(process.out.sam).match("bam_stub_sam") }, + { assert snapshot(process.out.versions).match("bam_stub_versions") } + ) + } + } +} diff --git a/modules/nf-core/samtools/view/tests/main.nf.test.snap b/modules/nf-core/samtools/view/tests/main.nf.test.snap new file mode 100644 index 0000000..6bcce9f --- /dev/null +++ b/modules/nf-core/samtools/view/tests/main.nf.test.snap @@ -0,0 +1,508 @@ +{ + "bam_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:51.256068" + }, + "cram_to_bam_index_csi": { + "content": [ + "test.bam.csi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:12.958617" + }, + "bam_stub_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:32.065301" + }, + "bam_bai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:51.258578" + }, + "bam_stub_bai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:32.071284" + }, + "bam_stub_versions": { + "content": [ + [ + "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:43:20.390692583" + }, + "cram_to_bam_index_cram": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:12.972288" + }, + "cram_to_bam_sam": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:04.999247" + }, + "cram_to_bam_index_sam": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:12.976457" + }, + "cram_crai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:56.497581" + }, + "cram_csi": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:56.50038" + }, + "cram_to_bam_cram": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:04.992239" + }, + "cram_to_bam_index_qname_csi": { + "content": [ + "test.bam.csi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.325496" + }, + "bam_stub_sam": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:32.079529" + }, + "cram_cram": { + "content": [ + "test.cram" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:56.490286" + }, + "bam_csi": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:51.262882" + }, + "cram_to_bam_crai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:04.989247" + }, + "cram_to_bam_index_crai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:12.967681" + }, + "cram_to_bam_index_qname_versions": { + "content": [ + [ + "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:43:15.007493874" + }, + "cram_to_bam_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:04.982361" + }, + "cram_to_bam_index_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:12.95456" + }, + "cram_to_bam_index_versions": { + "content": [ + [ + "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:43:09.472376824" + }, + "cram_to_bam_bai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:04.98601" + }, + "cram_to_bam_versions": { + "content": [ + [ + "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:43:04.080050906" + }, + "cram_bam": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:56.495512" + }, + "bam_stub_cram": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:32.076908" + }, + "cram_to_bam_index_qname_bai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.328458" + }, + "cram_to_bam_index_qname_crai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.330789" + }, + "cram_bai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:56.493129" + }, + "bam_stub_crai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:32.074313" + }, + "cram_to_bam_index_qname_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.322874" + }, + "cram_to_bam_index_qname_unselected": { + "content": [ + "test.unselected.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.322874" + }, + "cram_to_bam_index_qname_unselected_csi": { + "content": [ + "test.unselected.bam.csi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.328458" + }, + "bam_versions": { + "content": [ + [ + "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:42:52.978954857" + }, + "cram_to_bam_index_qname_cram": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.333248" + }, + "bam_crai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:51.259774" + }, + "bam_cram": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:51.261287" + }, + "cram_to_bam_csi": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:04.995454" + }, + "cram_sam": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:56.502625" + }, + "cram_versions": { + "content": [ + [ + "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-28T15:42:58.400776109" + }, + "bam_sam": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:51.264651" + }, + "cram_to_bam_index_bai": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:12.962863" + }, + "cram_to_bam_index_qname_sam": { + "content": [ + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.337634" + }, + "bam_stub_csi": { + "content": [ + "test.bam.csi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:32.068596" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/view/tests/tags.yml b/modules/nf-core/samtools/view/tests/tags.yml new file mode 100644 index 0000000..4fdf1dd --- /dev/null +++ b/modules/nf-core/samtools/view/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/view: + - "modules/nf-core/samtools/view/**" diff --git a/modules/nf-core/untar/environment.yml b/modules/nf-core/untar/environment.yml new file mode 100644 index 0000000..4f49824 --- /dev/null +++ b/modules/nf-core/untar/environment.yml @@ -0,0 +1,9 @@ +name: untar +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - conda-forge::grep=3.11 + - conda-forge::sed=4.8 + - conda-forge::tar=1.34 diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf index 8cd1856..9bd8f55 100644 --- a/modules/nf-core/untar/main.nf +++ b/modules/nf-core/untar/main.nf @@ -2,10 +2,10 @@ process UNTAR { tag "$archive" label 'process_single' - conda "conda-forge::sed=4.7 bioconda::grep=3.4 conda-forge::tar=1.34" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'nf-core/ubuntu:20.04' }" + 'https://depot.galaxyproject.org/singularity/ubuntu:22.04' : + 'nf-core/ubuntu:22.04' }" input: tuple val(meta), path(archive) @@ -52,8 +52,29 @@ process UNTAR { stub: prefix = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.toString().replaceFirst(/\.[^\.]+(.gz)?$/, "")) """ - mkdir $prefix - touch ${prefix}/file.txt + mkdir ${prefix} + ## Dry-run untaring the archive to get the files and place all in prefix + if [[ \$(tar -taf ${archive} | grep -o -P "^.*?\\/" | uniq | wc -l) -eq 1 ]]; then + for i in `tar -tf ${archive}`; + do + if [[ \$(echo "\${i}" | grep -E "/\$") == "" ]]; + then + touch \${i} + else + mkdir -p \${i} + fi + done + else + for i in `tar -tf ${archive}`; + do + if [[ \$(echo "\${i}" | grep -E "/\$") == "" ]]; + then + touch ${prefix}/\${i} + else + mkdir -p ${prefix}/\${i} + fi + done + fi cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/untar/meta.yml index db241a6..a9a2110 100644 --- a/modules/nf-core/untar/meta.yml +++ b/modules/nf-core/untar/meta.yml @@ -39,3 +39,8 @@ authors: - "@drpatelh" - "@matthdsm" - "@jfy133" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@matthdsm" + - "@jfy133" diff --git a/modules/nf-core/untar/tests/main.nf.test b/modules/nf-core/untar/tests/main.nf.test new file mode 100644 index 0000000..c957517 --- /dev/null +++ b/modules/nf-core/untar/tests/main.nf.test @@ -0,0 +1,85 @@ +nextflow_process { + + name "Test Process UNTAR" + script "../main.nf" + process "UNTAR" + tag "modules" + tag "modules_nfcore" + tag "untar" + + test("test_untar") { + + when { + process { + """ + input[0] = [ [], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/db/kraken2.tar.gz', checkIfExists: true) ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + ) + } + } + + test("test_untar_onlyfiles") { + + when { + process { + """ + input[0] = [ [], file(params.modules_testdata_base_path + 'generic/tar/hello.tar.gz', checkIfExists: true) ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + ) + } + } + + test("test_untar - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ [], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/db/kraken2.tar.gz', checkIfExists: true) ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + ) + } + } + + test("test_untar_onlyfiles - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ [], file(params.modules_testdata_base_path + 'generic/tar/hello.tar.gz', checkIfExists: true) ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + ) + } + } +} diff --git a/modules/nf-core/untar/tests/main.nf.test.snap b/modules/nf-core/untar/tests/main.nf.test.snap new file mode 100644 index 0000000..ceb91b7 --- /dev/null +++ b/modules/nf-core/untar/tests/main.nf.test.snap @@ -0,0 +1,158 @@ +{ + "test_untar_onlyfiles": { + "content": [ + { + "0": [ + [ + [ + + ], + [ + "hello.txt:md5,e59ff97941044f85df5297e1c302d260" + ] + ] + ], + "1": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ], + "untar": [ + [ + [ + + ], + [ + "hello.txt:md5,e59ff97941044f85df5297e1c302d260" + ] + ] + ], + "versions": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-10T12:04:28.231047" + }, + "test_untar_onlyfiles - stub": { + "content": [ + { + "0": [ + [ + [ + + ], + [ + "hello.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "1": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ], + "untar": [ + [ + [ + + ], + [ + "hello.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "versions": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-10T12:04:45.773103" + }, + "test_untar - stub": { + "content": [ + { + "0": [ + [ + [ + + ], + [ + "hash.k2d:md5,d41d8cd98f00b204e9800998ecf8427e", + "opts.k2d:md5,d41d8cd98f00b204e9800998ecf8427e", + "taxo.k2d:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "1": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ], + "untar": [ + [ + [ + + ], + [ + "hash.k2d:md5,d41d8cd98f00b204e9800998ecf8427e", + "opts.k2d:md5,d41d8cd98f00b204e9800998ecf8427e", + "taxo.k2d:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "versions": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-10T12:04:36.777441" + }, + "test_untar": { + "content": [ + { + "0": [ + [ + [ + + ], + [ + "hash.k2d:md5,8b8598468f54a7087c203ad0190555d9", + "opts.k2d:md5,a033d00cf6759407010b21700938f543", + "taxo.k2d:md5,094d5891cdccf2f1468088855c214b2c" + ] + ] + ], + "1": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ], + "untar": [ + [ + [ + + ], + [ + "hash.k2d:md5,8b8598468f54a7087c203ad0190555d9", + "opts.k2d:md5,a033d00cf6759407010b21700938f543", + "taxo.k2d:md5,094d5891cdccf2f1468088855c214b2c" + ] + ] + ], + "versions": [ + "versions.yml:md5,6063247258c56fd271d076bb04dd7536" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-10T12:04:19.377674" + } +} \ No newline at end of file diff --git a/modules/nf-core/untar/tests/tags.yml b/modules/nf-core/untar/tests/tags.yml new file mode 100644 index 0000000..feb6f15 --- /dev/null +++ b/modules/nf-core/untar/tests/tags.yml @@ -0,0 +1,2 @@ +untar: + - modules/nf-core/untar/** diff --git a/subworkflows/local/align_ont.nf b/subworkflows/local/align_ont.nf index 33d42b5..ef1a021 100644 --- a/subworkflows/local/align_ont.nf +++ b/subworkflows/local/align_ont.nf @@ -17,7 +17,7 @@ workflow ALIGN_ONT { // Align Fastq to Genome with minimap2. bam_format is set to true, making the output a *sorted* BAM - MINIMAP2_ALIGN ( reads, fasta, true, false, false ) + MINIMAP2_ALIGN ( reads, fasta, true, "bai", false, false ) ch_versions = ch_versions.mix ( MINIMAP2_ALIGN.out.versions.first() ) diff --git a/subworkflows/local/align_pacbio.nf b/subworkflows/local/align_pacbio.nf index 73a8321..f472a6c 100644 --- a/subworkflows/local/align_pacbio.nf +++ b/subworkflows/local/align_pacbio.nf @@ -24,10 +24,9 @@ workflow ALIGN_PACBIO { // Align Fastq to Genome with minimap2. bam_format is set to true, making the output a *sorted* BAM - MINIMAP2_ALIGN ( FILTER_PACBIO.out.fastq, fasta, true, false, false ) + MINIMAP2_ALIGN ( FILTER_PACBIO.out.fastq, fasta, true, "bai", false, false ) ch_versions = ch_versions.mix ( MINIMAP2_ALIGN.out.versions.first() ) - // Collect all alignment output by sample name MINIMAP2_ALIGN.out.bam | map { meta, bam -> [['id': meta.id.split('_')[0..-2].join('_'), 'datatype': meta.datatype], meta.read_count, bam] } diff --git a/subworkflows/local/align_short.nf b/subworkflows/local/align_short.nf index 8f061ad..6cb2eb0 100644 --- a/subworkflows/local/align_short.nf +++ b/subworkflows/local/align_short.nf @@ -25,7 +25,7 @@ workflow ALIGN_SHORT { // Align Fastq to Genome and output sorted BAM - BWAMEM2_MEM ( SAMTOOLS_FASTQ.out.fastq, index, true ) + BWAMEM2_MEM ( SAMTOOLS_FASTQ.out.fastq, index, fasta, true ) ch_versions = ch_versions.mix ( BWAMEM2_MEM.out.versions.first() ) diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index f1571ad..d76774e 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -101,7 +101,6 @@ workflow READMAPPING { if ( ch_reads.pacbio || ch_reads.clr ) { if ( params.vector_db.endsWith( '.tar.gz' ) ) { UNTAR ( [ [:], params.vector_db ] ).untar - | map { meta, file -> file } | set { ch_vector_db } ch_versions = ch_versions.mix ( UNTAR.out.versions ) From 089f2b71d7c452113c991f8d88729211e378c4d6 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 17 Jul 2024 11:16:33 +0100 Subject: [PATCH 054/111] samtools:1.17 -> 1.20 in local modules --- modules/local/samtools_collatetofasta.nf | 6 +++--- modules/local/samtools_filtertofastq.nf | 6 +++--- modules/local/samtools_replaceheader.nf | 6 +++--- modules/local/samtools_sormadup.nf | 6 +++--- 4 files changed, 12 insertions(+), 12 deletions(-) diff --git a/modules/local/samtools_collatetofasta.nf b/modules/local/samtools_collatetofasta.nf index 81119f2..6bed106 100644 --- a/modules/local/samtools_collatetofasta.nf +++ b/modules/local/samtools_collatetofasta.nf @@ -2,10 +2,10 @@ process SAMTOOLS_COLLATETOFASTA { tag "$meta.id" label 'process_medium' - conda "bioconda::samtools=1.17" + conda "bioconda::samtools=1.20" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : + 'biocontainers/samtools:1.20--h50ea8bc_0' }" input: tuple val(meta), path(input) diff --git a/modules/local/samtools_filtertofastq.nf b/modules/local/samtools_filtertofastq.nf index fbee087..09f158c 100644 --- a/modules/local/samtools_filtertofastq.nf +++ b/modules/local/samtools_filtertofastq.nf @@ -2,10 +2,10 @@ process SAMTOOLS_FILTERTOFASTQ { tag "$meta.id" label 'process_low' - conda "bioconda::samtools=1.17" + conda "bioconda::samtools=1.20" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : + 'biocontainers/samtools:1.20--h50ea8bc_0' }" input: tuple val(meta), path(input), path(index) diff --git a/modules/local/samtools_replaceheader.nf b/modules/local/samtools_replaceheader.nf index 09ac4fc..0dac256 100644 --- a/modules/local/samtools_replaceheader.nf +++ b/modules/local/samtools_replaceheader.nf @@ -2,10 +2,10 @@ process SAMTOOLS_REHEADER { tag "$meta.id" label 'process_single' - conda "bioconda::samtools=1.17" + conda "bioconda::samtools=1.20" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : + 'biocontainers/samtools:1.20--h50ea8bc_0' }" input: tuple val(meta), path(file) diff --git a/modules/local/samtools_sormadup.nf b/modules/local/samtools_sormadup.nf index 5aadab5..a403d9b 100644 --- a/modules/local/samtools_sormadup.nf +++ b/modules/local/samtools_sormadup.nf @@ -4,10 +4,10 @@ process SAMTOOLS_SORMADUP { tag "$meta.id" label 'process_medium' - conda "bioconda::samtools=1.17" + conda "bioconda::samtools=1.20" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : + 'biocontainers/samtools:1.20--h50ea8bc_0' }" input: tuple val(meta), path(input) From eea3db16a2f62607447b1176178eda990b266e89 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 17 Jul 2024 11:43:04 +0100 Subject: [PATCH 055/111] installing new nf-core subworkflows --- modules.json | 19 + nextflow.config | 5 +- .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 +++ .../tests/main.function.nf.test.snap | 20 + .../tests/main.workflow.nf.test | 111 +++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 446 ++++++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 ++++++ .../tests/main.function.nf.test.snap | 166 +++++++ .../tests/main.workflow.nf.test | 29 ++ .../tests/main.workflow.nf.test.snap | 19 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 +++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 ++++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + 22 files changed, 1616 insertions(+), 1 deletion(-) create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/modules.json b/modules.json index c995b54..1c665bd 100644 --- a/modules.json +++ b/modules.json @@ -82,6 +82,25 @@ "installed_by": ["modules"] } } + }, + "subworkflows": { + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + } + } } } } diff --git a/nextflow.config b/nextflow.config index b10fe94..fb742cd 100644 --- a/nextflow.config +++ b/nextflow.config @@ -142,7 +142,10 @@ profiles { test_full { includeConfig 'conf/test_full.config' } } - +// Nextflow plugins +plugins { + id 'nf-schema@2.0.0' // Validation of pipeline parameters and creation of an input channel from a sample sheet +} // Export these variables to prevent local Python/R libraries from conflicting with those in the container // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container. diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 0000000..ac31f28 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 0000000..e5c3a0a --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 0000000..68718e4 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 0000000..e3f0baf --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,20 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:05.308243" + }, + "Test Function checkCondaChannels": { + "content": null, + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:12.425833" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 0000000..ca964ce --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,111 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + workflow { + """ + print_version = false + dump_parameters = true + outdir = 'results' + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + workflow { + """ + print_version = false + dump_parameters = true + outdir = null + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 0000000..d0a926b --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 0000000..f847611 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 0000000..14558c3 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,446 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + def temp_doi_ref = "" + String[] manifest_doi = workflow.manifest.doi.tokenize(",") + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + for (String doi_ref: manifest_doi) temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + temp_doi_ref + "\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

$group

\n" + summary_section += "
\n" + for (param in group_params.keySet()) { + summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" + } + summary_section += "
\n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 0000000..d08d243 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 0000000..1dc317f --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 0000000..1037232 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,166 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:03.360873" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:59.729647" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:10.562934" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:07.019761" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:17.969323" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:14.366181" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:21.714424" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 0000000..8940d32 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 0000000..859d103 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,19 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:25.726491" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 0000000..d0a926b --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 0000000..ac8523c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 0000000..2585b65 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 0000000..3d4a6b0 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 0000000..5784a33 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 0000000..7626c1c --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 0000000..60b1cff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** From 6cfc64636d4a8e937ebfea4c84be64cb670d980d Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 17 Jul 2024 14:32:00 +0100 Subject: [PATCH 056/111] schema updates --- assets/schema_input.json | 14 ++++++++------ nextflow_schema.json | 12 +++++++++--- 2 files changed, 17 insertions(+), 9 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index 908328b..8832e91 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -14,18 +14,20 @@ }, "datatype": { "type": "string", - "pattern": "^\\S+$", - "errorMessage": "Data type, and must be one of: 'hic' or 'illumina' or 'ont' or 'pacbio'" + "enum": ["hic", "illumina", "pacbio", "pacbio_clr", "ont"], + "errorMessage": "Datatype must be one of: hic, illumina, pacbio, pacbio_clr, ont" }, "datafile": { - "type": "string", + "format": "file-path", + "exists": true, "pattern": "^\\S+$", - "errorMessage": "Data file for reads cannot contain spaces and must have extension 'cram' or 'bam' or '.fq.gz' or '.fastq.gz'" + "errorMessage": "Data file for reads cannot contain spaces and must have extension 'cram', 'bam', '.fq.gz' or '.fastq.gz'" }, "library": { "type": "string", - "pattern": "^\\S+$", - "errorMessage": "Library name to be assigned to read group (@RG) ID value" + "pattern": "^\\S*$", + "errorMessage": "Library information cannot contain spaces", + "default": "" } }, "required": ["sample", "datatype", "datafile"] diff --git a/nextflow_schema.json b/nextflow_schema.json index 20533b4..22f87c8 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -33,7 +33,7 @@ "type": "string", "description": "Path to directory or tar.gz archive for pre-built PacBio vector database.", "format": "path", - "default": "${projectDir}/assets/vectorDB.tar.gz", + "default": "/Users/tc25/projects/readmapping/assets/vectorDB.tar.gz", "fa_icon": "fas fa-bezier-curve" }, "email": { @@ -230,7 +230,7 @@ "tracedir": { "type": "string", "description": "Directory to keep pipeline Nextflow logs and reports.", - "default": "${params.outdir}/pipeline_info/readmapping", + "default": "./results/pipeline_info/readmapping", "fa_icon": "fas fa-cogs", "hidden": true }, @@ -270,5 +270,11 @@ { "$ref": "#/definitions/generic_options" } - ] + ], + "properties": { + "schema_ignore_params": { + "type": "string", + "default": "genomes" + } + } } From bdfb8b95f22578c21ea378ef91b52784e6708956 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Thu, 18 Jul 2024 14:29:06 +0100 Subject: [PATCH 057/111] nf_validation conversion --- assets/schema_input.json | 12 +- bin/check_samplesheet.py | 266 --------- lib/NfcoreSchema.groovy | 528 ------------------ lib/NfcoreTemplate.groovy | 317 ----------- lib/Utils.groovy | 47 -- lib/WorkflowMain.groovy | 87 --- lib/WorkflowReadmapping.groovy | 19 - lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes main.nf | 63 ++- nextflow.config | 2 +- nextflow_schema.json | 2 +- subworkflows/local/input_check.nf | 25 +- .../utils_nfcore_readmapping_pipeline/main.nf | 287 ++++++++++ workflows/readmapping.nf | 42 +- 14 files changed, 367 insertions(+), 1330 deletions(-) delete mode 100755 bin/check_samplesheet.py delete mode 100755 lib/NfcoreSchema.groovy delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100755 lib/Utils.groovy delete mode 100755 lib/WorkflowMain.groovy delete mode 100755 lib/WorkflowReadmapping.groovy delete mode 100644 lib/nfcore_external_java_deps.jar create mode 100644 subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf diff --git a/assets/schema_input.json b/assets/schema_input.json index 8832e91..16f0f28 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,24 +10,28 @@ "sample": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["sample"] }, "datatype": { "type": "string", "enum": ["hic", "illumina", "pacbio", "pacbio_clr", "ont"], - "errorMessage": "Datatype must be one of: hic, illumina, pacbio, pacbio_clr, ont" + "errorMessage": "Datatype must be one of: hic, illumina, pacbio, pacbio_clr, ont", + "meta": ["datatype"] }, "datafile": { "format": "file-path", "exists": true, "pattern": "^\\S+$", - "errorMessage": "Data file for reads cannot contain spaces and must have extension 'cram', 'bam', '.fq.gz' or '.fastq.gz'" + "errorMessage": "Data file for reads cannot contain spaces and must have extension 'cram', 'bam', '.fq.gz' or '.fastq.gz'", + "meta": ["datafile"] }, "library": { "type": "string", "pattern": "^\\S*$", "errorMessage": "Library information cannot contain spaces", - "default": "" + "default": "", + "meta": ["library"] } }, "required": ["sample", "datatype", "datafile"] diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index c6fdd5c..0000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,266 +0,0 @@ -#!/usr/bin/env python - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging -import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ".cram", - ".bam", - ) - - VALID_DATATYPES = ( - "hic", - "illumina", - "pacbio", - "pacbio_clr", - "ont", - ) - - def __init__( - self, - sample_col="sample", - type_col="datatype", - file_col="datafile", - lib_col="library", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - type_col (str): The name of the column that contains the dataype for - the read data (default "datatype"). - file_col (str): The name of the column that contains the file path for - the read data (default "datafile"). - lib_col (str): The name of the column that contains the library - information for the data (default "library"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._type_col = type_col - self._file_col = file_col - self._lib_col = lib_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_type(row) - self._validate_file(row) - self._seen.add((row[self._sample_col], row[self._file_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_type(self, row): - """Assert that the data type matches expected values.""" - if not any(row[self._type_col] for datatype in self.VALID_DATATYPES): - raise AssertionError( - f"The datatype is unrecognized: {row[self._type_col]}\n" - f"It should be one of: {', '.join(self.VALID_DATATYPES)}" - ) - - def _validate_file(self, row): - """Assert that the datafile is non-empty and has the right format.""" - if len(row[self._file_col]) <= 0: - raise AssertionError("Data file is required.") - self._validate_data_format(row[self._file_col]) - - def _validate_data_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The data file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different files, e.g., multiple runs per experiment. - - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" - - -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) - - -def sniff_format(handle): - """ - Detect the tabular format. - - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - if not sniffer.has_header(peek): - logger.critical("The given sample sheet does not appear to contain a header.") - sys.exit(1) - dialect = sniffer.sniff(peek) - return dialect - - -def check_samplesheet(file_in, file_out): - """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `readmapping samplesheet`_:: - - sample,datatype,datafile,library - sample1,hic,/path/to/file1.cram, - sample1,illumina,/path/to/file2.cram, - sample1,pacbio,/path/to/file1.bam, - sample1,ont,/path/to/file.fq.gz, - - .. _readmapping samplesheet: - https://raw.githubusercontent.com/sanger-tol/readmapping/main/assets/samplesheet.csv - - """ - required_columns = {"sample", "datatype", "datafile"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") - sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) - - -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - parser.add_argument( - "-v", - "--version", - action="version", - version="%(prog)s 1.0", - ) - return parser.parse_args(argv) - - -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy deleted file mode 100755 index 33cd4f6..0000000 --- a/lib/NfcoreSchema.groovy +++ /dev/null @@ -1,528 +0,0 @@ -// -// This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. -// - -import org.everit.json.schema.Schema -import org.everit.json.schema.loader.SchemaLoader -import org.everit.json.schema.ValidationException -import org.json.JSONObject -import org.json.JSONTokener -import org.json.JSONArray -import groovy.json.JsonSlurper -import groovy.json.JsonBuilder - -class NfcoreSchema { - - // - // Resolve Schema path relative to main workflow directory - // - public static String getSchemaPath(workflow, schema_filename='nextflow_schema.json') { - return "${workflow.projectDir}/${schema_filename}" - } - - // - // Function to loop over all parameters defined in schema and check - // whether the given parameters adhere to the specifications - // - /* groovylint-disable-next-line UnusedPrivateMethodParameter */ - public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') { - def has_error = false - //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~// - // Check for nextflow core params and unexpected params - def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text - def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions') - def nf_params = [ - // Options for base `nextflow` command - 'bg', - 'c', - 'C', - 'config', - 'd', - 'D', - 'dockerize', - 'h', - 'log', - 'q', - 'quiet', - 'syslog', - 'v', - - // Options for `nextflow run` command - 'ansi', - 'ansi-log', - 'bg', - 'bucket-dir', - 'c', - 'cache', - 'config', - 'dsl2', - 'dump-channels', - 'dump-hashes', - 'E', - 'entry', - 'latest', - 'lib', - 'main-script', - 'N', - 'name', - 'offline', - 'params-file', - 'pi', - 'plugins', - 'poll-interval', - 'pool-size', - 'profile', - 'ps', - 'qs', - 'queue-size', - 'r', - 'resume', - 'revision', - 'stdin', - 'stub', - 'stub-run', - 'test', - 'w', - 'with-charliecloud', - 'with-conda', - 'with-dag', - 'with-docker', - 'with-mpi', - 'with-notification', - 'with-podman', - 'with-report', - 'with-singularity', - 'with-timeline', - 'with-tower', - 'with-trace', - 'with-weblog', - 'without-docker', - 'without-podman', - 'work-dir' - ] - def unexpectedParams = [] - - // Collect expected parameters from the schema - def expectedParams = [] - def enums = [:] - for (group in schemaParams) { - for (p in group.value['properties']) { - expectedParams.push(p.key) - if (group.value['properties'][p.key].containsKey('enum')) { - enums[p.key] = group.value['properties'][p.key]['enum'] - } - } - } - - for (specifiedParam in params.keySet()) { - // nextflow params - if (nf_params.contains(specifiedParam)) { - log.error "ERROR: You used a core Nextflow option with two hyphens: '--${specifiedParam}'. Please resubmit with '-${specifiedParam}'" - has_error = true - } - // unexpected params - def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' - def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } - def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() - def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase)) - if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) { - // Temporarily remove camelCase/camel-case params #1035 - def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} - if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ - unexpectedParams.push(specifiedParam) - } - } - } - - //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~// - // Validate parameters against the schema - InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream() - JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream)) - - // Remove anything that's in params.schema_ignore_params - raw_schema = removeIgnoredParams(raw_schema, params) - - Schema schema = SchemaLoader.load(raw_schema) - - // Clean the parameters - def cleanedParams = cleanParameters(params) - - // Convert to JSONObject - def jsonParams = new JsonBuilder(cleanedParams) - JSONObject params_json = new JSONObject(jsonParams.toString()) - - // Validate - try { - schema.validate(params_json) - } catch (ValidationException e) { - println '' - log.error 'ERROR: Validation of pipeline parameters failed!' - JSONObject exceptionJSON = e.toJSON() - printExceptions(exceptionJSON, params_json, log, enums) - println '' - has_error = true - } - - // Check for unexpected parameters - if (unexpectedParams.size() > 0) { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - println '' - def warn_msg = 'Found unexpected parameters:' - for (unexpectedParam in unexpectedParams) { - warn_msg = warn_msg + "\n* --${unexpectedParam}: ${params[unexpectedParam].toString()}" - } - log.warn warn_msg - log.info "- ${colors.dim}Ignore this warning: params.schema_ignore_params = \"${unexpectedParams.join(',')}\" ${colors.reset}" - println '' - } - - if (has_error) { - System.exit(1) - } - } - - // - // Beautify parameters for --help - // - public static String paramsHelp(workflow, params, command, schema_filename='nextflow_schema.json') { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - Integer num_hidden = 0 - String output = '' - output += 'Typical pipeline command:\n\n' - output += " ${colors.cyan}${command}${colors.reset}\n\n" - Map params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) - Integer max_chars = paramsMaxChars(params_map) + 1 - Integer desc_indent = max_chars + 14 - Integer dec_linewidth = 160 - desc_indent - for (group in params_map.keySet()) { - Integer num_params = 0 - String group_output = colors.underlined + colors.bold + group + colors.reset + '\n' - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (group_params.get(param).hidden && !params.show_hidden_params) { - num_hidden += 1 - continue; - } - def type = '[' + group_params.get(param).type + ']' - def description = group_params.get(param).description - def defaultValue = group_params.get(param).default != null ? " [default: " + group_params.get(param).default.toString() + "]" : '' - def description_default = description + colors.dim + defaultValue + colors.reset - // Wrap long description texts - // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap - if (description_default.length() > dec_linewidth){ - List olines = [] - String oline = "" // " " * indent - description_default.split(" ").each() { wrd -> - if ((oline.size() + wrd.size()) <= dec_linewidth) { - oline += wrd + " " - } else { - olines += oline - oline = wrd + " " - } - } - olines += oline - description_default = olines.join("\n" + " " * desc_indent) - } - group_output += " --" + param.padRight(max_chars) + colors.dim + type.padRight(10) + colors.reset + description_default + '\n' - num_params += 1 - } - group_output += '\n' - if (num_params > 0){ - output += group_output - } - } - if (num_hidden > 0){ - output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset - } - output += NfcoreTemplate.dashedLine(params.monochrome_logs) - return output - } - - // - // Groovy Map summarising parameters/workflow options used by the pipeline - // - public static LinkedHashMap paramsSummaryMap(workflow, params, schema_filename='nextflow_schema.json') { - // Get a selection of core Nextflow workflow options - def Map workflow_summary = [:] - if (workflow.revision) { - workflow_summary['revision'] = workflow.revision - } - workflow_summary['runName'] = workflow.runName - if (workflow.containerEngine) { - workflow_summary['containerEngine'] = workflow.containerEngine - } - if (workflow.container) { - workflow_summary['container'] = workflow.container - } - workflow_summary['launchDir'] = workflow.launchDir - workflow_summary['workDir'] = workflow.workDir - workflow_summary['projectDir'] = workflow.projectDir - workflow_summary['userName'] = workflow.userName - workflow_summary['profile'] = workflow.profile - workflow_summary['configFiles'] = workflow.configFiles.join(', ') - - // Get pipeline parameters defined in JSON Schema - def Map params_summary = [:] - def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) - for (group in params_map.keySet()) { - def sub_params = new LinkedHashMap() - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (params.containsKey(param)) { - def params_value = params.get(param) - def schema_value = group_params.get(param).default - def param_type = group_params.get(param).type - if (schema_value != null) { - if (param_type == 'string') { - if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) { - def sub_string = schema_value.replace('\$projectDir', '') - sub_string = sub_string.replace('\${projectDir}', '') - if (params_value.contains(sub_string)) { - schema_value = params_value - } - } - if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) { - def sub_string = schema_value.replace('\$params.outdir', '') - sub_string = sub_string.replace('\${params.outdir}', '') - if ("${params.outdir}${sub_string}" == params_value) { - schema_value = params_value - } - } - } - } - - // We have a default in the schema, and this isn't it - if (schema_value != null && params_value != schema_value) { - sub_params.put(param, params_value) - } - // No default in the schema, and this isn't empty - else if (schema_value == null && params_value != "" && params_value != null && params_value != false) { - sub_params.put(param, params_value) - } - } - } - params_summary.put(group, sub_params) - } - return [ 'Core Nextflow options' : workflow_summary ] << params_summary - } - - // - // Beautify parameters for summary and return as string - // - public static String paramsSummaryLog(workflow, params) { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - String output = '' - def params_map = paramsSummaryMap(workflow, params) - def max_chars = paramsMaxChars(params_map) - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - if (group_params) { - output += colors.bold + group + colors.reset + '\n' - for (param in group_params.keySet()) { - output += " " + colors.blue + param.padRight(max_chars) + ": " + colors.green + group_params.get(param) + colors.reset + '\n' - } - output += '\n' - } - } - output += "!! Only displaying parameters that differ from the pipeline defaults !!\n" - output += NfcoreTemplate.dashedLine(params.monochrome_logs) - return output - } - - // - // Loop over nested exceptions and print the causingException - // - private static void printExceptions(ex_json, params_json, log, enums, limit=5) { - def causingExceptions = ex_json['causingExceptions'] - if (causingExceptions.length() == 0) { - def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/ - // Missing required param - if (m.matches()) { - log.error "* Missing required parameter: --${m[0][1]}" - } - // Other base-level error - else if (ex_json['pointerToViolation'] == '#') { - log.error "* ${ex_json['message']}" - } - // Error with specific param - else { - def param = ex_json['pointerToViolation'] - ~/^#\// - def param_val = params_json[param].toString() - if (enums.containsKey(param)) { - def error_msg = "* --${param}: '${param_val}' is not a valid choice (Available choices" - if (enums[param].size() > limit) { - log.error "${error_msg} (${limit} of ${enums[param].size()}): ${enums[param][0..limit-1].join(', ')}, ... )" - } else { - log.error "${error_msg}: ${enums[param].join(', ')})" - } - } else { - log.error "* --${param}: ${ex_json['message']} (${param_val})" - } - } - } - for (ex in causingExceptions) { - printExceptions(ex, params_json, log, enums) - } - } - - // - // Remove an element from a JSONArray - // - private static JSONArray removeElement(json_array, element) { - def list = [] - int len = json_array.length() - for (int i=0;i - if(raw_schema.keySet().contains('definitions')){ - raw_schema.definitions.each { definition -> - for (key in definition.keySet()){ - if (definition[key].get("properties").keySet().contains(ignore_param)){ - // Remove the param to ignore - definition[key].get("properties").remove(ignore_param) - // If the param was required, change this - if (definition[key].has("required")) { - def cleaned_required = removeElement(definition[key].required, ignore_param) - definition[key].put("required", cleaned_required) - } - } - } - } - } - if(raw_schema.keySet().contains('properties') && raw_schema.get('properties').keySet().contains(ignore_param)) { - raw_schema.get("properties").remove(ignore_param) - } - if(raw_schema.keySet().contains('required') && raw_schema.required.contains(ignore_param)) { - def cleaned_required = removeElement(raw_schema.required, ignore_param) - raw_schema.put("required", cleaned_required) - } - } - return raw_schema - } - - // - // Clean and check parameters relative to Nextflow native classes - // - private static Map cleanParameters(params) { - def new_params = params.getClass().newInstance(params) - for (p in params) { - // remove anything evaluating to false - if (!p['value']) { - new_params.remove(p.key) - } - // Cast MemoryUnit to String - if (p['value'].getClass() == nextflow.util.MemoryUnit) { - new_params.replace(p.key, p['value'].toString()) - } - // Cast Duration to String - if (p['value'].getClass() == nextflow.util.Duration) { - new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day")) - } - // Cast LinkedHashMap to String - if (p['value'].getClass() == LinkedHashMap) { - new_params.replace(p.key, p['value'].toString()) - } - } - return new_params - } - - // - // This function tries to read a JSON params file - // - private static LinkedHashMap paramsLoad(String json_schema) { - def params_map = new LinkedHashMap() - try { - params_map = paramsRead(json_schema) - } catch (Exception e) { - println "Could not read parameters settings from JSON. $e" - params_map = new LinkedHashMap() - } - return params_map - } - - // - // Method to actually read in JSON file using Groovy. - // Group (as Key), values are all parameters - // - Parameter1 as Key, Description as Value - // - Parameter2 as Key, Description as Value - // .... - // Group - // - - private static LinkedHashMap paramsRead(String json_schema) throws Exception { - def json = new File(json_schema).text - def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') - def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') - /* Tree looks like this in nf-core schema - * definitions <- this is what the first get('definitions') gets us - group 1 - title - description - properties - parameter 1 - type - description - parameter 2 - type - description - group 2 - title - description - properties - parameter 1 - type - description - * properties <- parameters can also be ungrouped, outside of definitions - parameter 1 - type - description - */ - - // Grouped params - def params_map = new LinkedHashMap() - schema_definitions.each { key, val -> - def Map group = schema_definitions."$key".properties // Gets the property object of the group - def title = schema_definitions."$key".title - def sub_params = new LinkedHashMap() - group.each { innerkey, value -> - sub_params.put(innerkey, value) - } - params_map.put(title, sub_params) - } - - // Ungrouped params - def ungrouped_params = new LinkedHashMap() - schema_properties.each { innerkey, value -> - ungrouped_params.put(innerkey, value) - } - params_map.put("Other parameters", ungrouped_params) - - return params_map - } - - // - // Get maximum number of characters across all parameter names - // - private static Integer paramsMaxChars(params_map) { - Integer max_chars = 0 - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (param.size() > max_chars) { - max_chars = param.size() - } - } - } - return max_chars - } -} diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index 4088387..0000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,317 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir" ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/readmapping/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // sanger-tol logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.blue} _____ ${colors.green} _______ ${colors.red} _${colors.reset} - ${colors.blue} / ____| ${colors.green}|__ __| ${colors.red}| |${colors.reset} - ${colors.blue} | (___ __ _ _ __ __ _ ___ _ __ ${colors.reset} ___ ${colors.green}| |${colors.yellow} ___ ${colors.red}| |${colors.reset} - ${colors.blue} \\___ \\ / _` | '_ \\ / _` |/ _ \\ '__|${colors.reset}|___|${colors.green}| |${colors.yellow}/ _ \\${colors.red}| |${colors.reset} - ${colors.blue} ____) | (_| | | | | (_| | __/ | ${colors.green}| |${colors.yellow} (_) ${colors.red}| |____${colors.reset} - ${colors.blue} |_____/ \\__,_|_| |_|\\__, |\\___|_| ${colors.green}|_|${colors.yellow}\\___/${colors.red}|______|${colors.reset} - ${colors.blue} __/ |${colors.reset} - ${colors.blue} |___/${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100755 index 8d030f4..0000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index e21f697..0000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,87 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the sanger-tol/readmapping pipeline -// - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - "* The pipeline\n" + - " https://doi.org/10.5281/zenodo.6563577\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/main/CITATIONS.md" - } - - // - // Generate help string - // - public static String help(workflow, params, log) { - def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --fasta reference.fa -profile docker" - def help_string = '' - help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs) - help_string += NfcoreSchema.paramsHelp(workflow, params, command) - help_string += '\n' + citation(workflow) + '\n' - help_string += NfcoreTemplate.dashedLine(params.monochrome_logs) - return help_string - } - - // - // Generate parameter summary log string - // - public static String paramsSummaryLog(workflow, params, log) { - def summary_log = '' - summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs) - summary_log += NfcoreSchema.paramsSummaryLog(workflow, params) - summary_log += '\n' + citation(workflow) + '\n' - summary_log += NfcoreTemplate.dashedLine(params.monochrome_logs) - return summary_log - } - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log) { - // Print help to screen if required - if (params.help) { - log.info help(workflow, params, log) - System.exit(0) - } - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Print parameter summary log to screen - log.info paramsSummaryLog(workflow, params, log) - - // Validate workflow parameters via the JSON schema - if (params.validate_params) { - NfcoreSchema.validateParameters(workflow, params, log) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - - // Check that conda channels are set-up correctly - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - log.error "Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'" - System.exit(1) - } - } -} diff --git a/lib/WorkflowReadmapping.groovy b/lib/WorkflowReadmapping.groovy deleted file mode 100755 index cf243a3..0000000 --- a/lib/WorkflowReadmapping.groovy +++ /dev/null @@ -1,19 +0,0 @@ -// -// This file holds several functions specific to the workflow/readmapping.nf in the sanger-tol/readmapping pipeline -// - -class WorkflowReadmapping { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - - if (!params.fasta) { - log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - System.exit(1) - } - - } - -} diff --git 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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -WorkflowMain.initialise(workflow, params, log) +include { READMAPPING } from './workflows/readmapping' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_readmapping_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_readmapping_pipeline' + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -23,13 +26,18 @@ WorkflowMain.initialise(workflow, params, log) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { READMAPPING } from './workflows/readmapping' - -// -// WORKFLOW: Run main sanger-tol/readmapping analysis pipeline -// workflow SANGERTOL_READMAPPING { - READMAPPING () + take: + samplesheet + fasta + header + + main: + READMAPPING ( + samplesheet, + fasta, + header + ) } /* @@ -38,12 +46,41 @@ workflow SANGERTOL_READMAPPING { ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// WORKFLOW: Execute a single named workflow for the pipeline -// See: https://github.com/nf-core/rnaseq/issues/619 -// workflow { - SANGERTOL_READMAPPING () + // + // SUBWORKFLOW: Run initialisation tasks + // + PIPELINE_INITIALISATION ( + params.version, + params.help, + params.validate_params, + params.monochrome_logs, + args, + params.outdir, + params.input + ) + + // + // WORKFLOW: Run main workflow + // + SANGERTOL_READMAPPING ( + PIPELINE_INITIALISATION.out.samplesheet, + PIPELINE_INITIALISATION.out.fasta, + PIPELINE_INITIALISATION.out.header + ) + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + [] + ) } /* diff --git a/nextflow.config b/nextflow.config index fb742cd..f3526aa 100644 --- a/nextflow.config +++ b/nextflow.config @@ -144,7 +144,7 @@ profiles { // Nextflow plugins plugins { - id 'nf-schema@2.0.0' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet } // Export these variables to prevent local Python/R libraries from conflicting with those in the container diff --git a/nextflow_schema.json b/nextflow_schema.json index 22f87c8..0e3d75e 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -33,7 +33,7 @@ "type": "string", "description": "Path to directory or tar.gz archive for pre-built PacBio vector database.", "format": "path", - "default": "/Users/tc25/projects/readmapping/assets/vectorDB.tar.gz", + "default": "assets/vectorDB.tar.gz", "fa_icon": "fas fa-bezier-curve" }, "email": { diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index d8a8a53..096e566 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -8,19 +8,15 @@ include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/samtools/flagstat/main workflow INPUT_CHECK { take: - samplesheet // file: /path/to/samplesheet.csv - + ch_samplesheet // channel: [ [meta], /path/to/reads ] main: ch_versions = Channel.empty() - // Read the samplesheet - SAMPLESHEET_CHECK ( samplesheet ).csv - | splitCsv ( header:true, sep:',' ) - // Prepare the channel for SAMTOOLS_FLAGSTAT - | map { row -> [row + [id: file(row.datafile).baseName], file(row.datafile, checkIfExists: true), []] } - | set { samplesheet_rows } - ch_versions = ch_versions.mix ( SAMPLESHEET_CHECK.out.versions.first() ) + // Prepare the samplesheet channel for SAMTOOLS_FLAGSTAT + ch_samplesheet + .map { meta, file -> [meta, file, []] } + .set { samplesheet_rows } // Get stats from each input file SAMTOOLS_FLAGSTAT ( samplesheet_rows ) @@ -46,14 +42,17 @@ def create_data_channel ( LinkedHashMap row, datafile, stats ) { meta.id = row.sample meta.datatype = row.datatype - if ( meta.datatype == "hic" || meta.datatype == "illumina" ) { + if ( meta.datatype == "hic" || meta.datatype == "illumina" ) { platform = "ILLUMINA" - } else if ( meta.datatype == "pacbio" || meta.datatype == "pacbio_clr" ) { + } else if ( meta.datatype == "pacbio" || meta.datatype == "pacbio_clr" ) { platform = "PACBIO" - } else if (meta.datatype == "ont") { + } else if (meta.datatype == "ont") { platform = "ONT" } - meta.read_group = "\'@RG\\tID:" + row.datafile.split('/')[-1].split('\\.')[0] + "\\tPL:" + platform + "\\tSM:" + meta.id.split('_')[0..-2].join('_') + "\'" + + // Convert datafile to string path and then split + def datafile_path = datafile.toString() + meta.read_group = "\'@RG\\tID:" + datafile_path.split('/')[-1].split('\\.')[0] + "\\tPL:" + platform + "\\tSM:" + meta.id.split('_')[0..-2].join('_') + "\'" // Read the first line of the flagstat file // 3127898040 + 0 in total (QC-passed reads + QC-failed reads) diff --git a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf new file mode 100644 index 0000000..cf4634f --- /dev/null +++ b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf @@ -0,0 +1,287 @@ +// +// Subworkflow with functionality specific to the {{ name }} pipeline +// + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { fromSamplesheet } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { logColours } from '../../nf-core/utils_nfcore_pipeline' +include { getWorkflowVersion } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir + input + + main: + + ch_versions = Channel.empty() + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = sangerTolLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + + // + // Custom validation for pipeline parameters + // + validateInputParameters() + + // Check input path parameters to see if they exist + def checkPathParamList = [ + params.input, + params.fasta, + params.vector_db, + params.bwamem2_index + ] + + for (param in checkPathParamList) { + if (param) { file(param, checkIfExists: true) } + } + + // Create channels from input paths + ch_fasta = params.fasta ? Channel.fromPath(params.fasta) : Channel.empty().tap { error 'Genome fasta file not specified!' } + ch_header = params.header ? Channel.fromPath(params.header) : Channel.empty() + + + // + // Create channel from input samplesheet + // + Channel + .fromSamplesheet("input") + .map { row -> + def meta = row[0] + [id: file(row[0].datafile).baseName] + return [meta, file(row[0].datafile, checkIfExists: true)] + } + .set { ch_samplesheet } + validateInputSamplesheet(ch_samplesheet) + .set { ch_validated_samplesheet } + + emit: + samplesheet = ch_validated_samplesheet + fasta = ch_fasta + header = ch_header + versions = ch_versions +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } + + workflow.onError { + log.error "Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting" + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + + +// +// Check and validate pipeline parameters +// +def validateInputParameters() { + + if (!params.fasta) { + log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." + System.exit(1) + } + +} + +// +// Validate channels from input samplesheet +// +def validateInputSamplesheet(channel) { + def seen = [:].withDefault { 0 } + def uniquePairs = new HashSet() + + // Use map to process each item in the channel + return channel.map { sample -> + def (meta, file) = sample + def pair = [meta.sample, meta.datafile].toString() + + if (!uniquePairs.add(pair)) { + error("The pair of sample name and read file must be unique: ${pair}") + } + + seen[meta.sample] += 1 + meta.sample = "${meta.sample}_T${seen[meta.sample]}" + + return [meta, file] + } +} + +// +// Sanger-ToL logo +// +def sangerTolLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.blue} _____ ${colors.green} _______ ${colors.red} _${colors.reset} + ${colors.blue} / ____| ${colors.green}|__ __| ${colors.red}| |${colors.reset} + ${colors.blue} | (___ __ _ _ __ __ _ ___ _ __ ${colors.reset} ___ ${colors.green}| |${colors.yellow} ___ ${colors.red}| |${colors.reset} + ${colors.blue} \\___ \\ / _` | '_ \\ / _` |/ _ \\ '__|${colors.reset}|___|${colors.green}| |${colors.yellow}/ _ \\${colors.red}| |${colors.reset} + ${colors.blue} ____) | (_| | | | | (_| | __/ | ${colors.green}| |${colors.yellow} (_) ${colors.red}| |____${colors.reset} + ${colors.blue} |_____/ \\__,_|_| |_|\\__, |\\___|_| ${colors.green}|_|${colors.yellow}\\___/${colors.red}|______|${colors.reset} + ${colors.blue} __/ |${colors.reset} + ${colors.blue} |___/${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + + +// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "

  • Author (2023) Pub name, Journal, DOI
  • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
  • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
  • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + if (meta.manifest_map.doi) { + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + def temp_doi_ref = "" + String[] manifest_doi = meta.manifest_map.doi.tokenize(",") + for (String doi_ref: manifest_doi) temp_doi_ref += "(doi:
    ${doi_ref.replace("https://doi.org/", "").replace(" ", "")}), " + meta["doi_text"] = temp_doi_ref.substring(0, temp_doi_ref.length() - 2) + } else meta["doi_text"] = "" + meta["nodoi_text"] = meta.manifest_map.doi ? "" : "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
  • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + + + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index d76774e..250ce7d 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -1,19 +1,3 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE INPUTS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) - -// Check input path parameters to see if they exist -def checkPathParamList = [ params.input, params.fasta, params.vector_db, params.bwamem2_index ] -for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } - -// Check mandatory parameters -if (params.input) { ch_input = Channel.fromPath(params.input) } else { exit 1, 'Input samplesheet not specified!' } -if (params.fasta) { ch_fasta = Channel.fromPath(params.fasta) } else { exit 1, 'Genome fasta file not specified!' } -if (params.header) { ch_header = Channel.fromPath(params.header) } /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -63,13 +47,19 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft workflow READMAPPING { - ch_versions = Channel.empty() + take: + ch_samplesheet + ch_fasta + ch_header + main: + // Initialize an empty versions channel + ch_versions = Channel.empty() // // SUBWORKFLOW: Read in samplesheet, validate and stage input files // - INPUT_CHECK ( ch_input ).reads + INPUT_CHECK ( ch_samplesheet ).reads | branch { meta, reads -> hic : meta.datatype == "hic" @@ -164,22 +154,6 @@ workflow READMAPPING { } -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -workflow.onComplete { - if ( params.email || params.email_on_fail ) { - NfcoreTemplate.email ( workflow, params, summary_params, projectDir, log ) - } - NfcoreTemplate.summary ( workflow, params, log ) - if ( params.hook_url ) { - NfcoreTemplate.IM_notification ( workflow, params, summary_params, projectDir, log ) - } -} - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From 28c683c186098400d97032da993ae995ebda0d3f Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 19 Jul 2024 11:25:45 +0100 Subject: [PATCH 058/111] added tool citations; removed old samplesheet_check --- modules/local/samplesheet_check.nf | 32 ------------------- subworkflows/local/input_check.nf | 2 -- .../utils_nfcore_readmapping_pipeline/main.nf | 24 ++++++++------ 3 files changed, 14 insertions(+), 44 deletions(-) delete mode 100644 modules/local/samplesheet_check.nf diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index f0a3073..0000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,32 +0,0 @@ -process SAMPLESHEET_CHECK { - tag "$samplesheet" - label 'process_single' - - conda "conda-forge::python=3.8.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" - - input: - path samplesheet - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // This script is bundled with the pipeline, in sanger-tol/readmapping/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - check_samplesheet.py: \$(check_samplesheet.py --version | cut -d' ' -f2) - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 096e566..f57d8e7 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -2,10 +2,8 @@ // Check input samplesheet and get read channels // -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/samtools/flagstat/main' - workflow INPUT_CHECK { take: ch_samplesheet // channel: [ [meta], /path/to/reads ] diff --git a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf index cf4634f..4cfd2d6 100644 --- a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf @@ -223,34 +223,38 @@ def sangerTolLogo(monochrome_logs=true) { // -// Generate methods description for MultiQC +// Generate methods description for tools // def toolCitationText() { - // TODO nf-core: Optionally add in-text citation tools to this list. // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", // Uncomment function in methodsDescriptionText to render in MultiQC report def citation_text = [ "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." + "blastn (Altschul et al. 1990),", + "bwa-mem2 (Vasimuddin et al. 2019),", + "crumble (Croucher et al. 2020),", + "MiniMap2 (Li 2018),", + "Samtools (Li et al. 2009)" ].join(' ').trim() return citation_text } def toolBibliographyText() { - // TODO nf-core: Optionally add bibliographic entries to this list. // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
  • " : "", // Uncomment function in methodsDescriptionText to render in MultiQC report def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
  • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
  • " + "
  • Altschul, S.F., Gish, W., Miller, W., Myers, E.W., & Lipman, D.J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403-410. doi:10.1016/S0022-2836(05)80360-2.
  • ", + "
  • Vasimuddin, Md., Misra, S., Li, H., & Aluru, S. (2019). Efficient Architecture-Aware Acceleration of BWA-MEM for Multicore Systems. IEEE Parallel and Distributed Processing Symposium (IPDPS), 2019. doi:10.1109/IPDPS.2019.00041.
  • ", + "
  • Croucher, N.J., Page, A.J., Connor, T.R., Delaney, A.J., Keane, J.A., & Bentley, S.D. (2020). Rapid phylogenetic analysis of large samples of recombinant bacterial whole genome sequences using Gubbins. Nucleic Acids Research, 43(3), e15. doi:10.1093/nar/gku1196.
  • ", + "
  • Li, H. (2018). Minimap2: pairwise alignment for nucleotide sequences. Bioinformatics, 34(18), 3094-3100. doi:10.1093/bioinformatics/bty191.
  • ", + "
  • Li, H., Handsaker, B., Wysoker, A., Fennell, T., Ruan, J., Homer, N., ... & Durbin, R. (2009). The Sequence Alignment/Map format and SAMtools. Bioinformatics, 25(16), 2078-2079. doi:10.1093/bioinformatics/btp352.
  • " ].join(' ').trim() return reference_text } + def methodsDescriptionText(mqc_methods_yaml) { // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file def meta = [:] @@ -274,8 +278,8 @@ def methodsDescriptionText(mqc_methods_yaml) { meta["tool_bibliography"] = "" // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - // meta["tool_bibliography"] = toolBibliographyText() + meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + meta["tool_bibliography"] = toolBibliographyText() def methods_text = mqc_methods_yaml.text From 004e03100e3158d3bb6cf490ec216ad51ce2a116 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 19 Jul 2024 11:40:04 +0100 Subject: [PATCH 059/111] corrected citations --- subworkflows/local/input_check.nf | 2 +- .../local/utils_nfcore_readmapping_pipeline/main.nf | 10 ++++------ 2 files changed, 5 insertions(+), 7 deletions(-) diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index f57d8e7..3465448 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -6,7 +6,7 @@ include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/samtools/flagstat/main workflow INPUT_CHECK { take: - ch_samplesheet // channel: [ [meta], /path/to/reads ] + ch_samplesheet // channel: [ val(meta), /path/to/reads ] main: ch_versions = Channel.empty() diff --git a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf index 4cfd2d6..aacdb31 100644 --- a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf @@ -230,9 +230,9 @@ def toolCitationText() { // Uncomment function in methodsDescriptionText to render in MultiQC report def citation_text = [ "Tools used in the workflow included:", - "blastn (Altschul et al. 1990),", + "blastn (Camacho et al. 2009),", "bwa-mem2 (Vasimuddin et al. 2019),", - "crumble (Croucher et al. 2020),", + "Crumble (Bonfield et al. 2019),", "MiniMap2 (Li 2018),", "Samtools (Li et al. 2009)" ].join(' ').trim() @@ -244,17 +244,15 @@ def toolBibliographyText() { // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
  • " : "", // Uncomment function in methodsDescriptionText to render in MultiQC report def reference_text = [ - "
  • Altschul, S.F., Gish, W., Miller, W., Myers, E.W., & Lipman, D.J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403-410. doi:10.1016/S0022-2836(05)80360-2.
  • ", + "
  • Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., & Madden, T.L. (2009). BLAST+: architecture and applications. BMC Bioinformatics, 10, 421. doi:10.1186/1471-2105-10-421.
  • ", "
  • Vasimuddin, Md., Misra, S., Li, H., & Aluru, S. (2019). Efficient Architecture-Aware Acceleration of BWA-MEM for Multicore Systems. IEEE Parallel and Distributed Processing Symposium (IPDPS), 2019. doi:10.1109/IPDPS.2019.00041.
  • ", - "
  • Croucher, N.J., Page, A.J., Connor, T.R., Delaney, A.J., Keane, J.A., & Bentley, S.D. (2020). Rapid phylogenetic analysis of large samples of recombinant bacterial whole genome sequences using Gubbins. Nucleic Acids Research, 43(3), e15. doi:10.1093/nar/gku1196.
  • ", + "
  • Bonfield, J.K., McCarthy, S.A., & Durbin, R. (2019). Crumble: reference free lossy compression of sequence quality values. Bioinformatics, 35(2), 337-339. doi:10.1093/bioinformatics/bty608.
  • ", "
  • Li, H. (2018). Minimap2: pairwise alignment for nucleotide sequences. Bioinformatics, 34(18), 3094-3100. doi:10.1093/bioinformatics/bty191.
  • ", "
  • Li, H., Handsaker, B., Wysoker, A., Fennell, T., Ruan, J., Homer, N., ... & Durbin, R. (2009). The Sequence Alignment/Map format and SAMtools. Bioinformatics, 25(16), 2078-2079. doi:10.1093/bioinformatics/btp352.
  • " ].join(' ').trim() return reference_text } - - def methodsDescriptionText(mqc_methods_yaml) { // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file def meta = [:] From 33527b8f7f021439316d153e19d18562a710431d Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 19 Jul 2024 12:50:52 +0100 Subject: [PATCH 060/111] add compliant template name --- .nf-core.yml | 2 ++ 1 file changed, 2 insertions(+) diff --git a/.nf-core.yml b/.nf-core.yml index 8031c1e..2d2f7d1 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -22,3 +22,5 @@ lint: nextflow_config: - manifest.name - manifest.homePage +template: + name: readmapping From 642ebab0f9915eab9df0768bfadee6c3b2af5781 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 19 Jul 2024 12:50:58 +0100 Subject: [PATCH 061/111] Template update for nf-core/tools version 2.14.1 --- .devcontainer/devcontainer.json | 11 +- .editorconfig | 13 +- .github/CONTRIBUTING.md | 34 +- .github/ISSUE_TEMPLATE/bug_report.yml | 95 ++-- .github/ISSUE_TEMPLATE/config.yml | 7 + .github/ISSUE_TEMPLATE/feature_request.yml | 2 +- .github/PULL_REQUEST_TEMPLATE.md | 10 +- .github/workflows/awsfulltest.yml | 39 ++ .github/workflows/awstest.yml | 33 ++ .github/workflows/branch.yml | 6 +- .github/workflows/ci.yml | 11 +- .github/workflows/clean-up.yml | 24 + .github/workflows/download_pipeline.yml | 86 +++ .github/workflows/fix-linting.yml | 74 ++- .github/workflows/linting.yml | 68 +-- .github/workflows/linting_comment.yml | 6 +- .github/workflows/release-announcements.yml | 75 +++ .github/workflows/sangerfulltest.yml | 39 -- .github/workflows/sangertest.yml | 23 - .gitpod.yml | 14 +- .nf-core.yml | 13 +- .pre-commit-config.yaml | 13 + CHANGELOG.md | 6 +- CITATIONS.md | 12 +- CODE_OF_CONDUCT.md | 182 ++++++ LICENSE | 2 +- README.md | 92 ++- assets/adaptivecard.json | 2 +- assets/email_template.html | 14 +- assets/email_template.txt | 16 +- assets/methods_description_template.yml | 18 +- assets/multiqc_config.yml | 17 + assets/nf-core-readmapping_logo_light.png | Bin 0 -> 107268 bytes assets/schema_input.json | 25 +- assets/sendmail_template.txt | 31 +- assets/slackreport.json | 2 +- bin/check_samplesheet.py | 262 --------- conf/base.config | 7 +- conf/igenomes.config | 440 +++++++++++++++ conf/modules.config | 21 +- conf/test.config | 8 +- conf/test_full.config | 8 +- docs/README.md | 6 +- docs/images/nf-core-readmapping_logo_dark.png | Bin 0 -> 27679 bytes .../images/nf-core-readmapping_logo_light.png | Bin 0 -> 23332 bytes docs/output.md | 46 +- docs/usage.md | 160 ++---- lib/NfcoreSchema.groovy | 528 ------------------ lib/NfcoreTemplate.groovy | 317 ----------- lib/Utils.groovy | 47 -- lib/WorkflowMain.groovy | 88 --- lib/WorkflowReadmapping.groovy | 21 - lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes main.nf | 91 ++- modules.json | 36 +- modules/local/samplesheet_check.nf | 31 - .../custom/dumpsoftwareversions/main.nf | 24 - .../custom/dumpsoftwareversions/meta.yml | 34 -- .../templates/dumpsoftwareversions.py | 91 --- modules/nf-core/fastqc/environment.yml | 7 + modules/nf-core/fastqc/main.nf | 61 ++ modules/nf-core/fastqc/meta.yml | 57 ++ modules/nf-core/fastqc/tests/main.nf.test | 212 +++++++ .../nf-core/fastqc/tests/main.nf.test.snap | 88 +++ modules/nf-core/fastqc/tests/tags.yml | 2 + modules/nf-core/multiqc/environment.yml | 7 + modules/nf-core/multiqc/main.nf | 55 ++ modules/nf-core/multiqc/meta.yml | 58 ++ modules/nf-core/multiqc/tests/main.nf.test | 84 +++ .../nf-core/multiqc/tests/main.nf.test.snap | 41 ++ modules/nf-core/multiqc/tests/tags.yml | 2 + nextflow.config | 230 +++++--- nextflow_schema.json | 108 +++- pyproject.toml | 10 - subworkflows/local/input_check.nf | 44 -- .../utils_nfcore_readmapping_pipeline/main.nf | 263 +++++++++ .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 ++ .../tests/main.function.nf.test.snap | 20 + .../tests/main.workflow.nf.test | 111 ++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 446 +++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 +++++ .../tests/main.function.nf.test.snap | 166 ++++++ .../tests/main.workflow.nf.test | 29 + .../tests/main.workflow.nf.test.snap | 19 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 ++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 +++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + tower.yml | 5 + workflows/readmapping.nf | 130 ++--- 98 files changed, 4308 insertions(+), 2130 deletions(-) create mode 100644 .github/ISSUE_TEMPLATE/config.yml create mode 100644 .github/workflows/awsfulltest.yml create mode 100644 .github/workflows/awstest.yml create mode 100644 .github/workflows/clean-up.yml create mode 100644 .github/workflows/download_pipeline.yml create mode 100644 .github/workflows/release-announcements.yml delete mode 100644 .github/workflows/sangerfulltest.yml delete mode 100644 .github/workflows/sangertest.yml create mode 100644 .pre-commit-config.yaml create mode 100644 CODE_OF_CONDUCT.md create mode 100644 assets/multiqc_config.yml create mode 100644 assets/nf-core-readmapping_logo_light.png delete mode 100755 bin/check_samplesheet.py create mode 100644 conf/igenomes.config create mode 100644 docs/images/nf-core-readmapping_logo_dark.png create mode 100644 docs/images/nf-core-readmapping_logo_light.png delete mode 100755 lib/NfcoreSchema.groovy delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100644 lib/Utils.groovy delete mode 100755 lib/WorkflowMain.groovy delete mode 100755 lib/WorkflowReadmapping.groovy delete mode 100644 lib/nfcore_external_java_deps.jar delete mode 100644 modules/local/samplesheet_check.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py create mode 100644 modules/nf-core/fastqc/environment.yml create mode 100644 modules/nf-core/fastqc/main.nf create mode 100644 modules/nf-core/fastqc/meta.yml create mode 100644 modules/nf-core/fastqc/tests/main.nf.test create mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastqc/tests/tags.yml create mode 100644 modules/nf-core/multiqc/environment.yml create mode 100644 modules/nf-core/multiqc/main.nf create mode 100644 modules/nf-core/multiqc/meta.yml create mode 100644 modules/nf-core/multiqc/tests/main.nf.test create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/multiqc/tests/tags.yml delete mode 100644 pyproject.toml delete mode 100644 subworkflows/local/input_check.nf create mode 100644 subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml create mode 100644 tower.yml diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index ea27a58..b290e09 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -2,6 +2,7 @@ "name": "nfcore", "image": "nfcore/gitpod:latest", "remoteUser": "gitpod", + "runArgs": ["--privileged"], // Configure tool-specific properties. "customizations": { @@ -9,15 +10,7 @@ "vscode": { // Set *default* container specific settings.json values on container create. "settings": { - "python.defaultInterpreterPath": "/opt/conda/bin/python", - "python.linting.enabled": true, - "python.linting.pylintEnabled": true, - "python.formatting.autopep8Path": "/opt/conda/bin/autopep8", - "python.formatting.yapfPath": "/opt/conda/bin/yapf", - "python.linting.flake8Path": "/opt/conda/bin/flake8", - "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", - "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.defaultInterpreterPath": "/opt/conda/bin/python" }, // Add the IDs of extensions you want installed when the container is created. diff --git a/.editorconfig b/.editorconfig index b78de6e..72dda28 100644 --- a/.editorconfig +++ b/.editorconfig @@ -8,7 +8,7 @@ trim_trailing_whitespace = true indent_size = 4 indent_style = space -[*.{md,yml,yaml,html,css,scss,js,cff}] +[*.{md,yml,yaml,html,css,scss,js}] indent_size = 2 # These files are edited and tested upstream in nf-core/modules @@ -18,7 +18,16 @@ end_of_line = unset insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset -indent_size = unset +[/subworkflows/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset [/assets/email*] indent_size = unset + +# ignore python and markdown +[*.{py,md}] +indent_style = unset diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 2999020..33d301a 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -1,20 +1,23 @@ -# sanger-tol/readmapping: Contributing Guidelines +# nf-core/readmapping: Contributing Guidelines Hi there! -Many thanks for taking an interest in improving sanger-tol/readmapping. +Many thanks for taking an interest in improving nf-core/readmapping. -We try to manage the required tasks for sanger-tol/readmapping using GitHub issues, you probably came to this page when creating one. +We try to manage the required tasks for nf-core/readmapping using GitHub issues, you probably came to this page when creating one. Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) +> [!NOTE] +> If you need help using or modifying nf-core/readmapping then the best place to ask is on the nf-core Slack [#readmapping](https://nfcore.slack.com/channels/readmapping) channel ([join our Slack here](https://nf-co.re/join/slack)). + ## Contribution workflow -If you'd like to write some code for sanger-tol/readmapping, the standard workflow is as follows: +If you'd like to write some code for nf-core/readmapping, the standard workflow is as follows: -1. Check that there isn't already an issue about your idea in the [sanger-tol/readmapping issues](https://github.com/sanger-tol/readmapping/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this -2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [sanger-tol/readmapping repository](https://github.com/sanger-tol/readmapping) to your GitHub account +1. Check that there isn't already an issue about your idea in the [nf-core/readmapping issues](https://github.com/nf-core/readmapping/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this +2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/readmapping repository](https://github.com/nf-core/readmapping) to your GitHub account 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions) 4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). 5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged @@ -23,6 +26,12 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests +You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: + +```bash +nf-test test --profile debug,test,docker --verbose +``` + When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -50,9 +59,13 @@ These tests are run both with the latest available version of `Nextflow` and als - Fix the bug, and bump version (X.Y.Z+1). - A PR should be made on `master` from patch to directly this particular bug. +## Getting help + +For further information/help, please consult the [nf-core/readmapping documentation](https://nf-co.re/readmapping/usage) and don't hesitate to get in touch on the nf-core Slack [#readmapping](https://nfcore.slack.com/channels/readmapping) channel ([join our Slack here](https://nf-co.re/join/slack)). + ## Pipeline contribution conventions -To make the sanger-tol/readmapping code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written. +To make the nf-core/readmapping code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written. ### Adding a new step @@ -66,6 +79,8 @@ If you wish to contribute a new step, please use the following coding standards: 6. Add sanity checks and validation for all relevant parameters. 7. Perform local tests to validate that the new code works as expected. 8. If applicable, add a new test command in `.github/workflow/ci.yml`. +9. Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module. +10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`. ### Default values @@ -77,7 +92,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. ### Naming schemes @@ -100,7 +115,7 @@ This repo includes a devcontainer configuration which will create a GitHub Codes To get started: -- Open the repo in [Codespaces](https://github.com/sanger-tol/readmapping/codespaces) +- Open the repo in [Codespaces](https://github.com/nf-core/readmapping/codespaces) - Tools installed - nf-core - Nextflow @@ -108,4 +123,3 @@ To get started: Devcontainer specs: - [DevContainer config](.devcontainer/devcontainer.json) -- [Dockerfile](.devcontainer/Dockerfile) diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index 1a40276..2b5a1e1 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -2,52 +2,49 @@ name: Bug report description: Report something that is broken or incorrect labels: bug body: -- type: textarea - id: description - attributes: - label: Description of the bug - description: A clear and concise description of what the bug is. - validations: - required: true -- type: textarea - id: command_used - attributes: - label: Command used and terminal output - description: Steps to reproduce the behaviour. Please paste the command you used - to launch the pipeline and the output from your terminal. - render: console - placeholder: '$ nextflow run ... - - - Some output where something broke - - ' -- type: textarea - id: files - attributes: - label: Relevant files - description: 'Please drag and drop the relevant files here. Create a `.zip` archive - if the extension is not allowed. - - Your verbose log file `.nextflow.log` is often useful _(this is a hidden file - in the directory where you launched the pipeline)_ as well as custom Nextflow - configuration files. - - ' -- type: textarea - id: system - attributes: - label: System information - description: '* Nextflow version _(eg. 22.10.1)_ - - * Hardware _(eg. HPC, Desktop, Cloud)_ - - * Executor _(eg. slurm, local, awsbatch)_ - - * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_ - - * OS _(eg. CentOS Linux, macOS, Linux Mint)_ - - * Version of sanger-tol/readmapping _(eg. 1.1, 1.5, 1.8.2)_ - - ' + - type: markdown + attributes: + value: | + Before you post this issue, please check the documentation: + + - [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting) + - [nf-core/readmapping pipeline documentation](https://nf-co.re/readmapping/usage) + + - type: textarea + id: description + attributes: + label: Description of the bug + description: A clear and concise description of what the bug is. + validations: + required: true + + - type: textarea + id: command_used + attributes: + label: Command used and terminal output + description: Steps to reproduce the behaviour. Please paste the command you used to launch the pipeline and the output from your terminal. + render: console + placeholder: | + $ nextflow run ... + + Some output where something broke + + - type: textarea + id: files + attributes: + label: Relevant files + description: | + Please drag and drop the relevant files here. Create a `.zip` archive if the extension is not allowed. + Your verbose log file `.nextflow.log` is often useful _(this is a hidden file in the directory where you launched the pipeline)_ as well as custom Nextflow configuration files. + + - type: textarea + id: system + attributes: + label: System information + description: | + * Nextflow version _(eg. 23.04.0)_ + * Hardware _(eg. HPC, Desktop, Cloud)_ + * Executor _(eg. slurm, local, awsbatch)_ + * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, or Apptainer)_ + * OS _(eg. CentOS Linux, macOS, Linux Mint)_ + * Version of nf-core/readmapping _(eg. 1.1, 1.5, 1.8.2)_ diff --git a/.github/ISSUE_TEMPLATE/config.yml b/.github/ISSUE_TEMPLATE/config.yml new file mode 100644 index 0000000..68bef12 --- /dev/null +++ b/.github/ISSUE_TEMPLATE/config.yml @@ -0,0 +1,7 @@ +contact_links: + - name: Join nf-core + url: https://nf-co.re/join + about: Please join the nf-core community here + - name: "Slack #readmapping channel" + url: https://nfcore.slack.com/channels/readmapping + about: Discussion about the nf-core/readmapping pipeline diff --git a/.github/ISSUE_TEMPLATE/feature_request.yml b/.github/ISSUE_TEMPLATE/feature_request.yml index f08da27..e787922 100644 --- a/.github/ISSUE_TEMPLATE/feature_request.yml +++ b/.github/ISSUE_TEMPLATE/feature_request.yml @@ -1,5 +1,5 @@ name: Feature request -description: Suggest an idea for the sanger-tol/readmapping pipeline +description: Suggest an idea for the nf-core/readmapping pipeline labels: enhancement body: - type: textarea diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index af196cc..0a7bfb4 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -1,23 +1,25 @@ ## PR checklist - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! -- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/sanger-tol/readmapping/tree/master/.github/CONTRIBUTING.md) +- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/readmapping/tree/master/.github/CONTRIBUTING.md) +- [ ] If necessary, also make a PR on the nf-core/readmapping _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. - [ ] `CHANGELOG.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml new file mode 100644 index 0000000..9bc2cb5 --- /dev/null +++ b/.github/workflows/awsfulltest.yml @@ -0,0 +1,39 @@ +name: nf-core AWS full size tests +# This workflow is triggered on published releases. +# It can be additionally triggered manually with GitHub actions workflow dispatch button. +# It runs the -profile 'test_full' on AWS batch + +on: + release: + types: [published] + workflow_dispatch: +jobs: + run-platform: + name: Run AWS full tests + if: github.repository == 'nf-core/readmapping' + runs-on: ubuntu-latest + steps: + - name: Launch workflow via Seqera Platform + uses: seqeralabs/action-tower-launch@v2 + # TODO nf-core: You can customise AWS full pipeline tests as required + # Add full size test data (but still relatively small datasets for few samples) + # on the `test_full.config` test runs with only one set of parameters + with: + workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} + access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} + compute_env: ${{ secrets.TOWER_COMPUTE_ENV }} + revision: ${{ github.sha }} + workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/readmapping/work-${{ github.sha }} + parameters: | + { + "hook_url": "${{ secrets.MEGATESTS_ALERTS_SLACK_HOOK_URL }}", + "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/readmapping/results-${{ github.sha }}" + } + profiles: test_full + + - uses: actions/upload-artifact@v4 + with: + name: Seqera Platform debug log file + path: | + seqera_platform_action_*.log + seqera_platform_action_*.json diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml new file mode 100644 index 0000000..6ec1cc3 --- /dev/null +++ b/.github/workflows/awstest.yml @@ -0,0 +1,33 @@ +name: nf-core AWS test +# This workflow can be triggered manually with the GitHub actions workflow dispatch button. +# It runs the -profile 'test' on AWS batch + +on: + workflow_dispatch: +jobs: + run-platform: + name: Run AWS tests + if: github.repository == 'nf-core/readmapping' + runs-on: ubuntu-latest + steps: + # Launch workflow using Seqera Platform CLI tool action + - name: Launch workflow via Seqera Platform + uses: seqeralabs/action-tower-launch@v2 + with: + workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} + access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} + compute_env: ${{ secrets.TOWER_COMPUTE_ENV }} + revision: ${{ github.sha }} + workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/readmapping/work-${{ github.sha }} + parameters: | + { + "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/readmapping/results-test-${{ github.sha }}" + } + profiles: test + + - uses: actions/upload-artifact@v4 + with: + name: Seqera Platform debug log file + path: | + seqera_platform_action_*.log + seqera_platform_action_*.json diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index d8a793b..df4999a 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -11,15 +11,15 @@ jobs: steps: # PRs to the nf-core repo master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches - name: Check PRs - if: github.repository == 'sanger-tol/readmapping' + if: github.repository == 'nf-core/readmapping' run: | - { [[ ${{github.event.pull_request.head.repo.full_name }} == sanger-tol/readmapping ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] + { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/readmapping ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] # If the above check failed, post a comment on the PR explaining the failure # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v1 + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 42a6200..618916d 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -19,22 +19,25 @@ jobs: test: name: Run pipeline with test data # Only run on push if this is the nf-core dev branch (merged PRs) - if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'sanger-tol/readmapping') }}" + if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/readmapping') }}" runs-on: ubuntu-latest strategy: matrix: NXF_VER: - - "22.10.1" + - "23.04.0" - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 with: version: "${{ matrix.NXF_VER }}" + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: Run pipeline with test data # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml new file mode 100644 index 0000000..0b6b1f2 --- /dev/null +++ b/.github/workflows/clean-up.yml @@ -0,0 +1,24 @@ +name: "Close user-tagged issues and PRs" +on: + schedule: + - cron: "0 0 * * 0" # Once a week + +jobs: + clean-up: + runs-on: ubuntu-latest + permissions: + issues: write + pull-requests: write + steps: + - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 + with: + stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." + stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." + close-issue-message: "This issue was closed because it has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor and then staled for 20 days with no activity." + days-before-stale: 30 + days-before-close: 20 + days-before-pr-close: -1 + any-of-labels: "awaiting-changes,awaiting-feedback" + exempt-issue-labels: "WIP" + exempt-pr-labels: "WIP" + repo-token: "${{ secrets.GITHUB_TOKEN }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml new file mode 100644 index 0000000..2d20d64 --- /dev/null +++ b/.github/workflows/download_pipeline.yml @@ -0,0 +1,86 @@ +name: Test successful pipeline download with 'nf-core download' + +# Run the workflow when: +# - dispatched manually +# - when a PR is opened or reopened to master branch +# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. +on: + workflow_dispatch: + inputs: + testbranch: + description: "The specific branch you wish to utilize for the test execution of nf-core download." + required: true + default: "dev" + pull_request: + types: + - opened + - edited + - synchronize + branches: + - master + pull_request_target: + branches: + - master + +env: + NXF_ANSI_LOG: false + +jobs: + download: + runs-on: ubuntu-latest + steps: + - name: Install Nextflow + uses: nf-core/setup-nextflow@v2 + + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 + with: + python-version: "3.12" + architecture: "x64" + - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 + with: + singularity-version: 3.8.3 + + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install git+https://github.com/nf-core/tools.git@dev + + - name: Get the repository name and current branch set as environment variable + run: | + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} + + - name: Download the pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + run: | + nf-core download ${{ env.REPO_LOWERCASE }} \ + --revision ${{ env.REPO_BRANCH }} \ + --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ + --compress "none" \ + --container-system 'singularity' \ + --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-cache-utilisation 'amend' \ + --download-configuration + + - name: Inspect download + run: tree ./${{ env.REPOTITLE_LOWERCASE }} + + - name: Run the downloaded pipeline (stub) + id: stub_run_pipeline + continue-on-error: true + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + - name: Run the downloaded pipeline (stub run not supported) + id: run_pipeline + if: ${{ job.steps.stub_run_pipeline.status == failure() }} + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 721ce3b..0e78a53 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -4,19 +4,26 @@ on: types: [created] jobs: - deploy: + fix-linting: # Only run if comment is on a PR with the main repo, and if it contains the magic keywords if: > contains(github.event.comment.html_url, '/pull/') && contains(github.event.comment.body, '@nf-core-bot fix linting') && - github.repository == 'sanger-tol/readmapping' + github.repository == 'nf-core/readmapping' runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v3 + - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} + # indication that the linting is being fixed + - name: React on comment + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: eyes + # Action runs on the issue comment, so we don't get the PR by default # Use the gh cli to check out the PR - name: Checkout Pull Request @@ -24,32 +31,59 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v3 + # Install and run pre-commit + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 + with: + python-version: "3.12" - - name: Install Prettier - run: npm install -g prettier @prettier/plugin-php + - name: Install pre-commit + run: pip install pre-commit - # Check that we actually need to fix something - - name: Run 'prettier --check' - id: prettier_status - run: | - if prettier --check ${GITHUB_WORKSPACE}; then - echo "name=result::pass" >> $GITHUB_OUTPUT - else - echo "name=result::fail" >> $GITHUB_OUTPUT - fi + - name: Run pre-commit + id: pre-commit + run: pre-commit run --all-files + continue-on-error: true - - name: Run 'prettier --write' - if: steps.prettier_status.outputs.result == 'fail' - run: prettier --write ${GITHUB_WORKSPACE} + # indication that the linting has finished + - name: react if linting finished succesfully + if: steps.pre-commit.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: "+1" - name: Commit & push changes - if: steps.prettier_status.outputs.result == 'fail' + id: commit-and-push + if: steps.pre-commit.outcome == 'failure' run: | git config user.email "core@nf-co.re" git config user.name "nf-core-bot" git config push.default upstream git add . git status - git commit -m "[automated] Fix linting with Prettier" + git commit -m "[automated] Fix code linting" git push + + - name: react if linting errors were fixed + id: react-if-fixed + if: steps.commit-and-push.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: hooray + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: confused + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + issue-number: ${{ github.event.issue.number }} + body: | + @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually. + See [CI log](https://github.com/nf-core/readmapping/actions/runs/${{ github.run_id }}) for more details. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 858d622..1fcafe8 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,74 +11,34 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - - uses: actions/setup-node@v3 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v3 - - - uses: actions/setup-node@v3 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v3 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.12 + uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: "3.12" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: "3.7" + python-version: "3.12" architecture: "x64" - name: Install dependencies @@ -99,7 +59,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 3963518..40acc23 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,17 +11,17 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v2 + uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3 with: workflow: linting.yml workflow_conclusion: completed - name: Get PR number id: pr_number - run: echo "name=pr_number::$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT + run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@v2 + uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml new file mode 100644 index 0000000..03ecfcf --- /dev/null +++ b/.github/workflows/release-announcements.yml @@ -0,0 +1,75 @@ +name: release-announcements +# Automatic release toot and tweet anouncements +on: + release: + types: [published] + workflow_dispatch: + +jobs: + toot: + runs-on: ubuntu-latest + steps: + - name: get topics and convert to hashtags + id: get_topics + run: | + echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" >> $GITHUB_OUTPUT + + - uses: rzr/fediverse-action@master + with: + access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} + host: "mstdn.science" # custom host if not "mastodon.social" (default) + # GitHub event payload + # https://docs.github.com/en/developers/webhooks-and-events/webhooks/webhook-events-and-payloads#release + message: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + + ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics + + send-tweet: + runs-on: ubuntu-latest + + steps: + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 + with: + python-version: "3.10" + - name: Install dependencies + run: pip install tweepy==4.14.0 + - name: Send tweet + shell: python + run: | + import os + import tweepy + + client = tweepy.Client( + access_token=os.getenv("TWITTER_ACCESS_TOKEN"), + access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"), + consumer_key=os.getenv("TWITTER_CONSUMER_KEY"), + consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"), + ) + tweet = os.getenv("TWEET") + client.create_tweet(text=tweet) + env: + TWEET: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }} + TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }} + TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }} + TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }} + + bsky-post: + runs-on: ubuntu-latest + steps: + - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0 + with: + post: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + env: + BSKY_IDENTIFIER: ${{ secrets.BSKY_IDENTIFIER }} + BSKY_PASSWORD: ${{ secrets.BSKY_PASSWORD }} + # diff --git a/.github/workflows/sangerfulltest.yml b/.github/workflows/sangerfulltest.yml deleted file mode 100644 index 38bcc5a..0000000 --- a/.github/workflows/sangerfulltest.yml +++ /dev/null @@ -1,39 +0,0 @@ - -name: sanger-tol LSF full size tests - -on: - push: - branches: - - main - - dev - workflow_dispatch: -jobs: - run-tower: - name: Run LSF full size tests - runs-on: ubuntu-latest - steps: - - - name: Sets env vars for push - run: | - echo "REVISION=${{ github.sha }}" >> $GITHUB_ENV - if: github.event_name == 'push' - - - name: Sets env vars for workflow_dispatch - run: | - echo "REVISION=${{ github.sha }}" >> $GITHUB_ENV - if: github.event_name == 'workflow_dispatch' - - - name: Launch workflow via tower - uses: nf-core/tower-action@v2 - with: - workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} - access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} - compute_env: ${{ secrets.TOWER_COMPUTE_ENV }} - pipeline: ${{ github.repository }} - revision: ${{ env.REVISION }} - workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/readmapping/work-${{ env.REVISION }} - parameters: | - { - "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/readmapping/results-${{ env.REVISION }}", - } - profiles: test_full,sanger,singularity diff --git a/.github/workflows/sangertest.yml b/.github/workflows/sangertest.yml deleted file mode 100644 index 3c89bca..0000000 --- a/.github/workflows/sangertest.yml +++ /dev/null @@ -1,23 +0,0 @@ -name: sanger-tol LSF tests - -on: - workflow_dispatch: -jobs: - run-tower: - name: Run LSF tests - runs-on: ubuntu-latest - steps: - - name: Launch workflow via tower - uses: nf-core/tower-action@v2 - with: - workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} - access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} - compute_env: ${{ secrets.TOWER_COMPUTE_ENV }} - pipeline: ${{ github.repository }} - revision: ${{ github.sha }} - workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/readmapping/work-${{ github.sha }} - parameters: | - { - "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/readmapping/results-${{ github.sha }}", - } - profiles: test,sanger,singularity diff --git a/.gitpod.yml b/.gitpod.yml index 85d95ec..105a182 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -1,14 +1,20 @@ image: nfcore/gitpod:latest +tasks: + - name: Update Nextflow and setup pre-commit + command: | + pre-commit install --install-hooks + nextflow self-update + - name: unset JAVA_TOOL_OPTIONS + command: | + unset JAVA_TOOL_OPTIONS vscode: extensions: # based on nf-core.nf-core-extensionpack - - codezombiech.gitignore # Language support for .gitignore files - # - cssho.vscode-svgviewer # SVG viewer - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code - - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar - mechatroner.rainbow-csv # Highlight columns in csv files in different colors - # - nextflow.nextflow # Nextflow syntax highlighting + # - nextflow.nextflow # Nextflow syntax highlighting - oderwat.indent-rainbow # Highlight indentation level - streetsidesoftware.code-spell-checker # Spelling checker for source code + - charliermarsh.ruff # Code linter Ruff diff --git a/.nf-core.yml b/.nf-core.yml index 421cc1c..770a543 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,11 +1,4 @@ +nf_core_version: 2.14.1 repository_type: pipeline -lint: - files_exist: - - CODE_OF_CONDUCT.md - - docs/images/nf-core-readmapping_logo.png - - .github/ISSUE_TEMPLATE/config.yml - - .github/workflows/sangertest.yml - - .github/workflows/sangerfulltest.yml - nextflow_config: - - manifest.name - - manifest.homePage +template: + name: readmapping diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml new file mode 100644 index 0000000..4dc0f1d --- /dev/null +++ b/.pre-commit-config.yaml @@ -0,0 +1,13 @@ +repos: + - repo: https://github.com/pre-commit/mirrors-prettier + rev: "v3.1.0" + hooks: + - id: prettier + additional_dependencies: + - prettier@3.2.5 + + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python + rev: "2.7.3" + hooks: + - id: editorconfig-checker + alias: ec diff --git a/CHANGELOG.md b/CHANGELOG.md index a8ab2e7..e10bcba 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,11 +1,11 @@ -# sanger-tol/readmapping: Changelog +# nf-core/readmapping: Changelog The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.1 - [date] +## v1.3.0 - [date] -Initial release of sanger-tol/readmapping, created with the [nf-core](https://nf-co.re/) template. +Initial release of nf-core/readmapping, created with the [nf-core](https://nf-co.re/) template. ### `Added` diff --git a/CITATIONS.md b/CITATIONS.md index 2162562..4e1340d 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -1,4 +1,4 @@ -# sanger-tol/readmapping: Citations +# nf-core/readmapping: Citations ## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) @@ -10,6 +10,13 @@ ## Pipeline tools +- [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) + + > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. + +- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) + + > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. ## Software packaging/containerisation tools @@ -27,5 +34,8 @@ - [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241) + > Merkel, D. (2014). Docker: lightweight linux containers for consistent development and deployment. Linux Journal, 2014(239), 2. doi: 10.5555/2600239.2600241. + - [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/) + > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675. diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md new file mode 100644 index 0000000..c089ec7 --- /dev/null +++ b/CODE_OF_CONDUCT.md @@ -0,0 +1,182 @@ +# Code of Conduct at nf-core (v1.4) + +## Our Pledge + +In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: + +- Age +- Ability +- Body size +- Caste +- Familial status +- Gender identity and expression +- Geographical location +- Level of experience +- Nationality and national origins +- Native language +- Neurodiversity +- Race or ethnicity +- Religion +- Sexual identity and orientation +- Socioeconomic status + +Please note that the list above is alphabetised and is therefore not ranked in any order of preference or importance. + +## Preamble + +:::note +This Code of Conduct (CoC) has been drafted by Renuka Kudva, Cris Tuñí, and Michael Heuer, with input from the nf-core Core Team and Susanna Marquez from the nf-core community. "We", in this document, refers to the Safety Officers and members of the nf-core Core Team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will be amended periodically to keep it up-to-date. In case of any dispute, the most current version will apply. +::: + +An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). + +Our Safety Officers are Saba Nafees, Cris Tuñí, and Michael Heuer. + +nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. + +We have therefore adopted this CoC, which we require all members of our community and attendees of nf-core events to adhere to in all our workspaces at all times. Workspaces include, but are not limited to, Slack, meetings on Zoom, gather.town, YouTube live etc. + +Our CoC will be strictly enforced and the nf-core team reserves the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. + +We ask all members of our community to help maintain supportive and productive workspaces and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. + +Questions, concerns, or ideas on what we can include? Contact members of the Safety Team on Slack or email safety [at] nf-co [dot] re. + +## Our Responsibilities + +Members of the Safety Team (the Safety Officers) are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. + +The Safety Team, in consultation with the nf-core core team, have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this CoC, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. + +Members of the core team or the Safety Team who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and will be subject to the same actions as others in violation of the CoC. + +## When and where does this Code of Conduct apply? + +Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events, such as hackathons, workshops, bytesize, and collaborative workspaces on gather.town. These guidelines include, but are not limited to, the following (listed alphabetically and therefore in no order of preference): + +- Communicating with an official project email address. +- Communicating with community members within the nf-core Slack channel. +- Participating in hackathons organised by nf-core (both online and in-person events). +- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence, and on the nf-core gather.town workspace. +- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, gather.town, Jitsi, YouTube live etc. +- Representing nf-core on social media. This includes both official and personal accounts. + +## nf-core cares 😊 + +nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include, but are not limited to, the following (listed in alphabetical order): + +- Ask for consent before sharing another community member’s personal information (including photographs) on social media. +- Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. +- Celebrate your accomplishments! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) +- Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) +- Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) +- Focus on what is best for the team and the community. (When in doubt, ask) +- Accept feedback, yet be unafraid to question, deliberate, and learn. +- Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) +- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communication to be kind.**) +- Take breaks when you feel like you need them. +- Use welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack) + +## nf-core frowns on 😕 + +The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this CoC. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces: + +- Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. +- “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. +- Spamming or trolling of individuals on social media. +- Use of sexual or discriminatory imagery, comments, jokes, or unwelcome sexual attention. +- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion, or work experience. + +### Online Trolling + +The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the risk of online trolling. This is unacceptable — reports of such behaviour will be taken very seriously and perpetrators will be excluded from activities immediately. + +All community members are **required** to ask members of the group they are working with for explicit consent prior to taking screenshots of individuals during video calls. + +## Procedures for reporting CoC violations + +If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. + +You can reach out to members of the Safety Team (Saba Nafees, Cris Tuñí, and Michael Heuer) on Slack. Alternatively, contact a member of the nf-core core team [nf-core core team](https://nf-co.re/about), and they will forward your concerns to the Safety Team. + +Issues directly concerning members of the Core Team or the Safety Team will be dealt with by other members of the core team and the safety manager — possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson and details will be shared in due course. + +All reports will be handled with the utmost discretion and confidentiality. + +You can also report any CoC violations to safety [at] nf-co [dot] re. In your email report, please do your best to include: + +- Your contact information. +- Identifying information (e.g. names, nicknames, pseudonyms) of the participant who has violated the Code of Conduct. +- The behaviour that was in violation and the circumstances surrounding the incident. +- The approximate time of the behaviour (if different than the time the report was made). +- Other people involved in the incident, if applicable. +- If you believe the incident is ongoing. +- If there is a publicly available record (e.g. mailing list record, a screenshot). +- Any additional information. + +After you file a report, one or more members of our Safety Team will contact you to follow up on your report. + +## Who will read and handle reports + +All reports will be read and handled by the members of the Safety Team at nf-core. + +If members of the Safety Team are deemed to have a conflict of interest with a report, they will be required to recuse themselves as per our Code of Conduct and will not have access to any follow-ups. + +To keep this first report confidential from any of the Safety Team members, please submit your first report by direct messaging on Slack/direct email to any of the nf-core members you are comfortable disclosing the information to, and be explicit about which member(s) you do not consent to sharing the information with. + +## Reviewing reports + +After receiving the report, members of the Safety Team will review the incident report to determine whether immediate action is required, for example, whether there is immediate threat to participants’ safety. + +The Safety Team, in consultation with members of the nf-core core team, will assess the information to determine whether the report constitutes a Code of Conduct violation, for them to decide on a course of action. + +In the case of insufficient information, one or more members of the Safety Team may contact the reporter, the reportee, or any other attendees to obtain more information. + +Once additional information is gathered, the Safety Team will collectively review and decide on the best course of action to take, if any. The Safety Team reserves the right to not act on a report. + +## Confidentiality + +All reports, and any additional information included, are only shared with the team of safety officers (and possibly members of the core team, in case the safety officer is in violation of the CoC). We will respect confidentiality requests for the purpose of protecting victims of abuse. + +We will not name harassment victims, beyond discussions between the safety officer and members of the nf-core team, without the explicit consent of the individuals involved. + +## Enforcement + +Actions taken by the nf-core’s Safety Team may include, but are not limited to: + +- Asking anyone to stop a behaviour. +- Asking anyone to leave the event and online spaces either temporarily, for the remainder of the event, or permanently. +- Removing access to the gather.town and Slack, either temporarily or permanently. +- Communicating to all participants to reinforce our expectations for conduct and remind what is unacceptable behaviour; this may be public for practical reasons. +- Communicating to all participants that an incident has taken place and how we will act or have acted — this may be for the purpose of letting event participants know we are aware of and dealing with the incident. +- Banning anyone from participating in nf-core-managed spaces, future events, and activities, either temporarily or permanently. +- No action. + +## Attribution and Acknowledgements + +- The [Contributor Covenant, version 1.4](http://contributor-covenant.org/version/1/4) +- The [OpenCon 2017 Code of Conduct](http://www.opencon2017.org/code_of_conduct) (CC BY 4.0 OpenCon organisers, SPARC and Right to Research Coalition) +- The [eLife innovation sprint 2020 Code of Conduct](https://sprint.elifesciences.org/code-of-conduct/) +- The [Mozilla Community Participation Guidelines v3.1](https://www.mozilla.org/en-US/about/governance/policies/participation/) (version 3.1, CC BY-SA 3.0 Mozilla) + +## Changelog + +### v1.4 - February 8th, 2022 + +- Included a new member of the Safety Team. Corrected a typographical error in the text. + +### v1.3 - December 10th, 2021 + +- Added a statement that the CoC applies to nf-core gather.town workspaces. Corrected typographical errors in the text. + +### v1.2 - November 12th, 2021 + +- Removed information specific to reporting CoC violations at the Hackathon in October 2021. + +### v1.1 - October 14th, 2021 + +- Updated with names of new Safety Officers and specific information for the hackathon in October 2021. + +### v1.0 - March 15th, 2021 + +- Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/LICENSE b/LICENSE index d878e86..e238724 100644 --- a/LICENSE +++ b/LICENSE @@ -1,6 +1,6 @@ MIT License -Copyright (c) 2022 Genome Research Ltd. +Copyright (c) @priyanka-surana Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal diff --git a/README.md b/README.md index f303b62..64bab94 100644 --- a/README.md +++ b/README.md @@ -1,58 +1,86 @@ -[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) - -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.10.1-23aa62.svg)](https://www.nextflow.io/) +

    + + + nf-core/readmapping + +

    + +[![GitHub Actions CI Status](https://github.com/nf-core/readmapping/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/readmapping/actions/workflows/ci.yml) +[![GitHub Actions Linting Status](https://github.com/nf-core/readmapping/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/readmapping/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/readmapping/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) + +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/sanger-tol/readmapping) +[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/readmapping) + +[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23readmapping-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/readmapping)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) ## Introduction - +**nf-core/readmapping** is a bioinformatics pipeline that ... + + -**sanger-tol/readmapping** is a bioinformatics best-practice analysis pipeline for map reads to genome. + + -The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! +1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) - +## Usage -On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. +> [!NOTE] +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. -## Pipeline summary + +First, prepare a samplesheet with your input data that looks as follows: +`samplesheet.csv`: -## Quick Start +```csv +sample,fastq_1,fastq_2 +CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz +``` -1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=22.10.1`) +Each row represents a fastq file (single-end) or a pair of fastq files (paired end). -2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) (you can follow [this tutorial](https://singularity-tutorial.github.io/01-installation/)), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(you can use [`Conda`](https://conda.io/miniconda.html) both to install Nextflow itself and also to manage software within pipelines. Please only use it within pipelines as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_. +--> -3. Download the pipeline and test it on a minimal dataset with a single command: +Now, you can run the pipeline using: - ```bash - nextflow run sanger-tol/readmapping -profile test,YOURPROFILE --outdir - ``` + - Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string. +```bash +nextflow run nf-core/readmapping \ + -profile \ + --input samplesheet.csv \ + --outdir +``` - > - The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`. - > - Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. - > - If you are using `singularity`, please use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs. - > - If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs. +> [!WARNING] +> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; +> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). -4. Start running your own analysis! +For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/readmapping/usage) and the [parameter documentation](https://nf-co.re/readmapping/parameters). - +## Pipeline output - ```bash - nextflow run sanger-tol/readmapping --input samplesheet.csv --outdir --genome GRCh37 -profile - ``` +To see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/readmapping/results) tab on the nf-core website pipeline page. +For more details about the output files and reports, please refer to the +[output documentation](https://nf-co.re/readmapping/output). ## Credits -sanger-tol/readmapping was originally written by @priyanka-surana. +nf-core/readmapping was originally written by @priyanka-surana. We thank the following people for their extensive assistance in the development of this pipeline: @@ -62,16 +90,18 @@ We thank the following people for their extensive assistance in the development If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md). +For further information or help, don't hesitate to get in touch on the [Slack `#readmapping` channel](https://nfcore.slack.com/channels/readmapping) (you can join with [this invite](https://nf-co.re/join/slack)). + ## Citations - + An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. -This pipeline uses code and infrastructure developed and maintained by the [nf-core](https://nf-co.re) community, reused here under the [MIT license](https://github.com/nf-core/tools/blob/master/LICENSE). +You can cite the `nf-core` publication as follows: > **The nf-core framework for community-curated bioinformatics pipelines.** > diff --git a/assets/adaptivecard.json b/assets/adaptivecard.json index 4cf56a6..73e7fa1 100644 --- a/assets/adaptivecard.json +++ b/assets/adaptivecard.json @@ -17,7 +17,7 @@ "size": "Large", "weight": "Bolder", "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", - "text": "sanger-tol/readmapping v${version} - ${runName}", + "text": "nf-core/readmapping v${version} - ${runName}", "wrap": true }, { diff --git a/assets/email_template.html b/assets/email_template.html index 5c0fae1..00e284a 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -4,21 +4,21 @@ - - sanger-tol/readmapping Pipeline Report + + nf-core/readmapping Pipeline Report
    -

    sanger-tol/readmapping v${version}

    +

    nf-core/readmapping ${version}

    Run Name: $runName

    <% if (!success){ out << """
    -

    sanger-tol/readmapping execution completed unsuccessfully!

    +

    nf-core/readmapping execution completed unsuccessfully!

    The exit status of the task that caused the workflow execution to fail was: $exitStatus.

    The full error message was:

    ${errorReport}
    @@ -27,7 +27,7 @@

    sanger-tol/readmapping execution compl } else { out << """
    - sanger-tol/readmapping execution completed successfully! + nf-core/readmapping execution completed successfully!
    """ } @@ -44,8 +44,8 @@

    Pipeline Configuration:

    Process Name \\", + " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    -

    sanger-tol/readmapping

    -

    https://github.com/sanger-tol/readmapping

    +

    nf-core/readmapping

    +

    https://github.com/nf-core/readmapping

    diff --git a/assets/email_template.txt b/assets/email_template.txt index 89f6f33..9e15bc6 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -1,10 +1,18 @@ +---------------------------------------------------- + ,--./,-. + ___ __ __ __ ___ /,-._.--~\\ + |\\ | |__ __ / ` / \\ |__) |__ } { + | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, + `._,._,' + nf-core/readmapping ${version} +---------------------------------------------------- Run Name: $runName <% if (success){ - out << "## sanger-tol/readmapping execution completed successfully! ##" + out << "## nf-core/readmapping execution completed successfully! ##" } else { out << """#################################################### -## sanger-tol/readmapping execution completed unsuccessfully! ## +## nf-core/readmapping execution completed unsuccessfully! ## #################################################### The exit status of the task that caused the workflow execution to fail was: $exitStatus. The full error message was: @@ -27,5 +35,5 @@ Pipeline Configuration: <% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> -- -sanger-tol/readmapping -https://github.com/sanger-tol/readmapping +nf-core/readmapping +https://github.com/nf-core/readmapping diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml index ab1d0cc..adf3c95 100644 --- a/assets/methods_description_template.yml +++ b/assets/methods_description_template.yml @@ -1,19 +1,23 @@ -id: "sanger-tol-readmapping-methods-description" +id: "nf-core-readmapping-methods-description" description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." -section_name: "sanger-tol/readmapping Methods Description" -section_href: "https://github.com/sanger-tol/readmapping" +section_name: "nf-core/readmapping Methods Description" +section_href: "https://github.com/nf-core/readmapping" plot_type: "html" -## TODO nf-core: Update the HTML below to your prefered methods description, e.g. add publication citation for this pipeline +## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline ## You inject any metadata in the Nextflow '${workflow}' object data: |

    Methods

    -

    Data was processed using sanger-tol/readmapping v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020).

    +

    Data was processed using nf-core/readmapping v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

    The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

    ${workflow.commandLine}
    +

    ${tool_citations}

    References

      -
    • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. https://doi.org/10.1038/nbt.3820
    • -
    • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. https://doi.org/10.1038/s41587-020-0439-x
    • +
    • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
    • +
    • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
    • +
    • Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
    • +
    • da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
    • + ${tool_bibliography}
    Notes:
    diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml new file mode 100644 index 0000000..4aa8ced --- /dev/null +++ b/assets/multiqc_config.yml @@ -0,0 +1,17 @@ +report_comment: > + + This report has been generated by the nf-core/readmapping + analysis pipeline. 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z%^M=z0V-Pa-w2HVA&C5YNs=>qIn32K2C`@ZHN8_9{n!6&(Hnbrp=|oH;*_F_`^r4j zw_505_&#tOej9foQ7BahjGk9zU2OC}H}b$s#@ky5SmnUdG)*&<^~PR)1UkGftHZ1` z$%3{tje~|!h2H5a844uQMn|G{2VVjTb#k!H;E#IC+ECc~e-DKl{Fhc=oMffK58#py zaqg<#RCRT5=W8nIL3ip;Y*Tf#k404$MuGQ)_9mru&^KBi;QBBuk;%fD|2f3DYPAs5 zwQE`pO%ih>?FR<;WbJ6httwWA@xwWD)PMhfUzGio6T?NLSzWCWHqhTzSwGSuIPVFl zPQJktMmIO7?EUJ?wh55laW^YXW%np(q9{+^1-~Gd7THXoF7bz##3r?A3)m>48r7a? zROm;;^(_G<)mBN+mc5G?4}ZB)V0riKv`LV&8(I`Zw@Lf2w2$T#ICqW0iyEQEQ9l73n;nGDv_+J|13B$?V*aBkxSlW+%gMUbrk9PQxa0f zz3KKyWnm)NuPJkP&oIBe>P#%v{E3VJ zYpJ!1^)2PqyM;Fw`em)kQ>z`+a?=-q+UqXc#jk6&Q{Pa$0=Ha^m_80?=`y}=jIvsi z!&9^Dv1R1&%N%5f%^2?a4fi*?QB=vphm4k$iMkyWtVYjkj%$qzYKj`_J8pB2HrG&5LvQJ1 z(6*-J6Px9;xqi;Zs}33cQ1V&#@Z{>!B!_&h@%9_qbkl5;gS4fZ1!kooI@#m2Q1C)b zDY{yRT?JN3&t?T)5fe#Qy;Y=uM#j literal 0 HcmV?d00001 diff --git a/assets/schema_input.json b/assets/schema_input.json index ee63131..957870a 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,7 +1,7 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/sanger-tol/readmapping/master/assets/schema_input.json", - "title": "sanger-tol/readmapping pipeline - params.input schema", + "$id": "https://raw.githubusercontent.com/nf-core/readmapping/master/assets/schema_input.json", + "title": "nf-core/readmapping pipeline - params.input schema", "description": "Schema for the file provided with params.input", "type": "array", "items": { @@ -10,25 +10,22 @@ "sample": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] }, "fastq_1": { "type": "string", + "format": "file-path", + "exists": true, "pattern": "^\\S+\\.f(ast)?q\\.gz$", "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" }, "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" } }, "required": ["sample", "fastq_1"] diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt index 7d6915a..e35b36d 100644 --- a/assets/sendmail_template.txt +++ b/assets/sendmail_template.txt @@ -9,12 +9,12 @@ Content-Type: text/html; charset=utf-8 $email_html --nfcoremimeboundary -Content-Type: image/png;name="sanger-tol-readmapping_logo.png" +Content-Type: image/png;name="nf-core-readmapping_logo.png" Content-Transfer-Encoding: base64 Content-ID: -Content-Disposition: inline; filename="sanger-tol-readmapping_logo.png" +Content-Disposition: inline; filename="nf-core-readmapping_logo_light.png" -<% out << new File("$projectDir/docs/images/nf-core-readmapping_logo.png"). +<% out << new File("$projectDir/assets/nf-core-readmapping_logo_light.png"). bytes. encodeBase64(). toString(). @@ -25,4 +25,29 @@ Content-Disposition: inline; filename="sanger-tol-readmapping_logo.png" flatten(). join( '\n' ) %> +<% +if (mqcFile){ +def mqcFileObj = new File("$mqcFile") +if (mqcFileObj.length() < mqcMaxSize){ +out << """ +--nfcoremimeboundary +Content-Type: text/html; name=\"multiqc_report\" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" + +${mqcFileObj. + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' )} +""" +}} +%> + --nfcoremimeboundary-- diff --git a/assets/slackreport.json b/assets/slackreport.json index 043d02f..0eb4bdb 100644 --- a/assets/slackreport.json +++ b/assets/slackreport.json @@ -3,7 +3,7 @@ { "fallback": "Plain-text summary of the attachment.", "color": "<% if (success) { %>good<% } else { %>danger<%} %>", - "author_name": "sanger-tol/readmapping v${version} - ${runName}", + "author_name": "nf-core/readmapping ${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index 11b1557..0000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,262 +0,0 @@ -#!/usr/bin/env python - - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging -import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ) - - def __init__( - self, - sample_col="sample", - first_col="fastq_1", - second_col="fastq_2", - single_col="single_end", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - first_col (str): The name of the column that contains the first (or only) - FASTQ file path (default "fastq_1"). - second_col (str): The name of the column that contains the second (if any) - FASTQ file path (default "fastq_2"). - single_col (str): The name of the new column that will be inserted and - records whether the sample contains single- or paired-end sequencing - reads (default "single_end"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._first_col = first_col - self._second_col = second_col - self._single_col = single_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_first(row) - self._validate_second(row) - self._validate_pair(row) - self._seen.add((row[self._sample_col], row[self._first_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_first(self, row): - """Assert that the first FASTQ entry is non-empty and has the right format.""" - if len(row[self._first_col]) <= 0: - raise AssertionError("At least the first FASTQ file is required.") - self._validate_fastq_format(row[self._first_col]) - - def _validate_second(self, row): - """Assert that the second FASTQ entry has the right format if it exists.""" - if len(row[self._second_col]) > 0: - self._validate_fastq_format(row[self._second_col]) - - def _validate_pair(self, row): - """Assert that read pairs have the same file extension. Report pair status.""" - if row[self._first_col] and row[self._second_col]: - row[self._single_col] = False - first_col_suffix = Path(row[self._first_col]).suffixes[-2:] - second_col_suffix = Path(row[self._second_col]).suffixes[-2:] - if first_col_suffix != second_col_suffix: - raise AssertionError("FASTQ pairs must have the same file extensions.") - else: - row[self._single_col] = True - - def _validate_fastq_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The FASTQ file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. - - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" - - -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) - - -def sniff_format(handle): - """ - Detect the tabular format. - - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - if not sniffer.has_header(peek): - logger.critical("The given sample sheet does not appear to contain a header.") - sys.exit(1) - dialect = sniffer.sniff(peek) - return dialect - - -def check_samplesheet(file_in, file_out): - """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. Also add - an additional column which records whether one or two FASTQ reads were found. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - - """ - required_columns = {"sample", "fastq_1", "fastq_2"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") - sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - header.insert(1, "single_end") - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) - - -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - return parser.parse_args(argv) - - -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/conf/base.config b/conf/base.config index c9e022d..af9dd02 100644 --- a/conf/base.config +++ b/conf/base.config @@ -1,6 +1,6 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - sanger-tol/readmapping Nextflow base config file + nf-core/readmapping Nextflow base config file ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ A 'blank slate' config file, appropriate for general use on most high performance compute environments. Assumes that all software is installed and available on @@ -15,7 +15,7 @@ process { memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 4.h * task.attempt, 'time' ) } - errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } + errorStrategy = { task.exitStatus in ((130..145) + 104) ? 'retry' : 'finish' } maxRetries = 1 maxErrors = '-1' @@ -59,7 +59,4 @@ process { errorStrategy = 'retry' maxRetries = 2 } - withName:CUSTOM_DUMPSOFTWAREVERSIONS { - cache = false - } } diff --git a/conf/igenomes.config b/conf/igenomes.config new file mode 100644 index 0000000..3f11437 --- /dev/null +++ b/conf/igenomes.config @@ -0,0 +1,440 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for iGenomes paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines reference genomes using iGenome paths. + Can be used by any config that customises the base path using: + $params.igenomes_base / --igenomes_base +---------------------------------------------------------------------------------------- +*/ + +params { + // illumina iGenomes reference file paths + genomes { + 'GRCh37' { + fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" + } + 'GRCh38' { + fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" + } + 'CHM13' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAIndex/" + bwamem2 = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAmem2Index/" + gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/CHM13/Annotation/Genes/genes.gtf" + gff = "ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/009/914/755/GCF_009914755.1_T2T-CHM13v2.0/GCF_009914755.1_T2T-CHM13v2.0_genomic.gff.gz" + mito_name = "chrM" + } + 'GRCm38' { + fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.87e9" + blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" + } + 'TAIR10' { + fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" + mito_name = "Mt" + } + 'EB2' { + fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" + } + 'UMD3.1' { + fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" + mito_name = "MT" + } + 'WBcel235' { + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" + mito_name = "MtDNA" + macs_gsize = "9e7" + } + 'CanFam3.1' { + fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" + mito_name = "MT" + } + 'GRCz10' { + fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'BDGP6' { + fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" + mito_name = "M" + macs_gsize = "1.2e8" + } + 'EquCab2' { + fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" + mito_name = "MT" + } + 'EB1' { + fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" + } + 'Galgal4' { + fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'Gm01' { + fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" + } + 'Mmul_1' { + fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" + mito_name = "MT" + } + 'IRGSP-1.0' { + fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" + mito_name = "Mt" + } + 'CHIMP2.1.4' { + fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" + mito_name = "MT" + } + 'Rnor_5.0' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'Rnor_6.0' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'R64-1-1' { + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" + mito_name = "MT" + macs_gsize = "1.2e7" + } + 'EF2' { + fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.21e7" + } + 'Sbi1' { + fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" + } + 'Sscrofa10.2' { + fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" + mito_name = "MT" + } + 'AGPv3' { + fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" + mito_name = "Mt" + } + 'hg38' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" + } + 'hg19' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" + } + 'mm10' { + fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.87e9" + blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" + } + 'bosTau8' { + fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'ce10' { + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "9e7" + } + 'canFam3' { + fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" + mito_name = "chrM" + } + 'danRer10' { + fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "1.37e9" + } + 'dm6' { + fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "1.2e8" + } + 'equCab2' { + fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" + mito_name = "chrM" + } + 'galGal4' { + fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" + mito_name = "chrM" + } + 'panTro4' { + fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" + mito_name = "chrM" + } + 'rn6' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'sacCer3' { + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" + readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.2e7" + } + 'susScr3' { + fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" + mito_name = "chrM" + } + } +} diff --git a/conf/modules.config b/conf/modules.config index 014c49e..d203d2b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -12,19 +12,22 @@ process { - withName: SAMPLESHEET_CHECK { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + publishDir = [ + path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + + withName: FASTQC { + ext.args = '--quiet' } - withName: CUSTOM_DUMPSOFTWAREVERSIONS { + withName: 'MULTIQC' { + ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ - path: { "${params.outdir}/pipeline_info" }, + path: { "${params.outdir}/multiqc" }, mode: params.publish_dir_mode, - pattern: '*_versions.yml' + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } diff --git a/conf/test.config b/conf/test.config index 7a795a7..e1c7131 100644 --- a/conf/test.config +++ b/conf/test.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run sanger-tol/readmapping -profile test, --outdir + nextflow run nf-core/readmapping -profile test, --outdir ---------------------------------------------------------------------------------------- */ @@ -22,8 +22,8 @@ params { // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' - // Fasta references - fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/genome/NC_045512.2/GCF_009858895.2_ASM985889v3_genomic.200409.fna.gz' + // Genome references + genome = 'R64-1-1' } diff --git a/conf/test_full.config b/conf/test_full.config index e5ff6cb..cfc396a 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a full size pipeline test. Use as follows: - nextflow run sanger-tol/readmapping -profile test_full, --outdir + nextflow run nf-core/readmapping -profile test_full, --outdir ---------------------------------------------------------------------------------------- */ @@ -17,8 +17,8 @@ params { // Input data for full size test // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' + input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' - // Fasta references - fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/genome/NC_045512.2/GCF_009858895.2_ASM985889v3_genomic.200409.fna.gz' + // Genome references + genome = 'R64-1-1' } diff --git a/docs/README.md b/docs/README.md index 87f5ca0..c22fb7f 100644 --- a/docs/README.md +++ b/docs/README.md @@ -1,8 +1,10 @@ -# sanger-tol/readmapping: Documentation +# nf-core/readmapping: Documentation -The sanger-tol/readmapping documentation is split into the following pages: +The nf-core/readmapping documentation is split into the following pages: - [Usage](usage.md) - An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. - [Output](output.md) - An overview of the different results produced by the pipeline and how to interpret them. + +You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: [https://nf-co.re](https://nf-co.re) diff --git a/docs/images/nf-core-readmapping_logo_dark.png b/docs/images/nf-core-readmapping_logo_dark.png new file mode 100644 index 0000000000000000000000000000000000000000..469674c195998e42d6de25aa296075ebc34ea10d GIT binary patch literal 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z{gae?=iVY}=AhkwXP38vwzxr$yqLJz6|y%RByjyt4=>gM^f|e?U&3Gy4e4@Vua8Vu zJmWa}J_qc(X`R|^kSy~(rf;f2-u6|^*r0RQknte(afc_FE#hKwjrWs3&E+df1ziiN zo^B`kB$W51r0h8&Nf<;oof7-{-Tyj9BWje=YCvViGCd*-BD_l|NfVqfAYm85SN+}p zRsuI0yLZRw@_%C3u50+Zuz?f5T?~&yUIw`XQs<*Y<1+q6!JT{Tf0U LSRiXIyWaah2~7cx literal 0 HcmV?d00001 diff --git a/docs/output.md b/docs/output.md index 1163973..bfe228d 100644 --- a/docs/output.md +++ b/docs/output.md @@ -1,8 +1,8 @@ -# sanger-tol/readmapping: Output +# nf-core/readmapping: Output ## Introduction -This document describes the output produced by the pipeline. +This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. @@ -12,8 +12,49 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: +- [FastQC](#fastqc) - Raw read QC +- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution +### FastQC + +
    +Output files + +- `fastqc/` + - `*_fastqc.html`: FastQC report containing quality metrics. + - `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. + +
    + +[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). + +![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) + +![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) + +![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) + +:::note +The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. +::: + +### MultiQC + +
    +Output files + +- `multiqc/` + - `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. + - `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. + - `multiqc_plots/`: directory containing static images from the report in various formats. + +
    + +[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. + +Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . + ### Pipeline information
    @@ -23,6 +64,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline. - Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. + - Parameters used by the pipeline run: `params.json`.
    diff --git a/docs/usage.md b/docs/usage.md index 77a35c8..510ca75 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -1,4 +1,6 @@ -# sanger-tol/readmapping: Usage +# nf-core/readmapping: Usage + +## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/readmapping/usage](https://nf-co.re/readmapping/usage) > _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ @@ -18,7 +20,7 @@ You will need to create a samplesheet with information about the samples you wou The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz @@ -31,7 +33,7 @@ The pipeline will auto-detect whether a sample is single- or paired-end using th A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz @@ -55,7 +57,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```bash -nextflow run sanger-tol/readmapping --input samplesheet.csv --outdir --genome GRCh37 -profile docker +nextflow run nf-core/readmapping --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -69,33 +71,68 @@ work # Directory containing the nextflow working files # Other nextflow hidden files, eg. history of pipeline runs and old logs. ``` +If you wish to repeatedly use the same parameters for multiple runs, rather than specifying each flag in the command, you can specify these in a params file. + +Pipeline settings can be provided in a `yaml` or `json` file via `-params-file `. + +:::warning +Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). +::: + +The above pipeline run specified with a params file in yaml format: + +```bash +nextflow run nf-core/readmapping -profile docker -params-file params.yaml +``` + +with `params.yaml` containing: + +```yaml +input: './samplesheet.csv' +outdir: './results/' +genome: 'GRCh37' +<...> +``` + +You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-co.re/launch). + ### Updating the pipeline When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: ```bash -nextflow pull sanger-tol/readmapping +nextflow pull nf-core/readmapping ``` ### Reproducibility It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. -First, go to the [sanger-tol/readmapping releases page](https://github.com/sanger-tol/readmapping/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. +First, go to the [nf-core/readmapping releases page](https://github.com/nf-core/readmapping/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. + +This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports. -This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. +To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter. + +:::tip +If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles. +::: ## Core Nextflow arguments -> **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). +:::note +These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). +::: ### `-profile` Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. -Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. +Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below. -> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. +:::info +We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. +::: The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). @@ -117,8 +154,12 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof - A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/) - `charliecloud` - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) +- `apptainer` + - A generic configuration profile to be used with [Apptainer](https://apptainer.org/) +- `wave` + - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. Use together with one of the above (requires Nextflow ` 24.03.0-edge` or later). - `conda` - - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud. + - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer. ### `-resume` @@ -136,102 +177,19 @@ Specify the path to a specific config file (this is a core Nextflow command). Se Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. -For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue: - -```console -[62/149eb0] NOTE: Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1) -Error executing process > 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)' - -Caused by: - Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) - -Command executed: - STAR \ - --genomeDir star \ - --readFilesIn WT_REP1_trimmed.fq.gz \ - --runThreadN 2 \ - --outFileNamePrefix WT_REP1. \ - - -Command exit status: - 137 - -Command output: - (empty) - -Command error: - .command.sh: line 9: 30 Killed STAR --genomeDir star --readFilesIn WT_REP1_trimmed.fq.gz --runThreadN 2 --outFileNamePrefix WT_REP1. -Work dir: - /home/pipelinetest/work/9d/172ca5881234073e8d76f2a19c88fb - -Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run` -``` - -#### For beginners - -A first step to bypass this error, you could try to increase the amount of CPUs, memory, and time for the whole pipeline. Therefor you can try to increase the resource for the parameters `--max_cpus`, `--max_memory`, and `--max_time`. Based on the error above, you have to increase the amount of memory. Therefore you can go to the [parameter documentation of rnaseq](https://nf-co.re/rnaseq/3.9/parameters) and scroll down to the `show hidden parameter` button to get the default value for `--max_memory`. In this case 128GB, you than can try to run your pipeline again with `--max_memory 200GB -resume` to skip all process, that were already calculated. If you can not increase the resource of the complete pipeline, you can try to adapt the resource for a single process as mentioned below. - -#### Advanced option on process level - -To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). -We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so, based on the search results, the file we want is `modules/nf-core/star/align/main.nf`. -If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). -The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. -The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. -Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. -The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. - -```nextflow -process { - withName: 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN' { - memory = 100.GB - } -} -``` - -> **NB:** We specify the full process name i.e. `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden. -> -> If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly. - -### Updating containers (advanced users) - -The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`. - -1. Check the default version used by the pipeline in the module file for [Pangolin](https://github.com/nf-core/viralrecon/blob/a85d5969f9025409e3618d6c280ef15ce417df65/modules/nf-core/software/pangolin/main.nf#L14-L19) -2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags) -3. Create the custom config accordingly: - - - For Docker: +To change the resource requests, please see the [max resources](https://nf-co.re/docs/usage/configuration#max-resources) and [tuning workflow resources](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources) section of the nf-core website. - ```nextflow - process { - withName: PANGOLIN { - container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0' - } - } - ``` +### Custom Containers - - For Singularity: +In some cases you may wish to change which container or conda environment a step of the pipeline uses for a particular tool. By default nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. However in some cases the pipeline specified version maybe out of date. - ```nextflow - process { - withName: PANGOLIN { - container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0' - } - } - ``` +To use a different container from the default container or conda environment specified in a pipeline, please see the [updating tool versions](https://nf-co.re/docs/usage/configuration#updating-tool-versions) section of the nf-core website. - - For Conda: +### Custom Tool Arguments - ```nextflow - process { - withName: PANGOLIN { - conda = 'bioconda::pangolin=3.0.5' - } - } - ``` +A pipeline might not always support every possible argument or option of a particular tool used in pipeline. Fortunately, nf-core pipelines provide some freedom to users to insert additional parameters that the pipeline does not include by default. -> **NB:** If you wish to periodically update individual tool-specific results (e.g. Pangolin) generated by the pipeline then you must ensure to keep the `work/` directory otherwise the `-resume` ability of the pipeline will be compromised and it will restart from scratch. +To learn how to provide additional arguments to a particular tool of the pipeline, please see the [customising tool arguments](https://nf-co.re/docs/usage/configuration#customising-tool-arguments) section of the nf-core website. ### nf-core/configs diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy deleted file mode 100755 index 33cd4f6..0000000 --- a/lib/NfcoreSchema.groovy +++ /dev/null @@ -1,528 +0,0 @@ -// -// This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. -// - -import org.everit.json.schema.Schema -import org.everit.json.schema.loader.SchemaLoader -import org.everit.json.schema.ValidationException -import org.json.JSONObject -import org.json.JSONTokener -import org.json.JSONArray -import groovy.json.JsonSlurper -import groovy.json.JsonBuilder - -class NfcoreSchema { - - // - // Resolve Schema path relative to main workflow directory - // - public static String getSchemaPath(workflow, schema_filename='nextflow_schema.json') { - return "${workflow.projectDir}/${schema_filename}" - } - - // - // Function to loop over all parameters defined in schema and check - // whether the given parameters adhere to the specifications - // - /* groovylint-disable-next-line UnusedPrivateMethodParameter */ - public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') { - def has_error = false - //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~// - // Check for nextflow core params and unexpected params - def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text - def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions') - def nf_params = [ - // Options for base `nextflow` command - 'bg', - 'c', - 'C', - 'config', - 'd', - 'D', - 'dockerize', - 'h', - 'log', - 'q', - 'quiet', - 'syslog', - 'v', - - // Options for `nextflow run` command - 'ansi', - 'ansi-log', - 'bg', - 'bucket-dir', - 'c', - 'cache', - 'config', - 'dsl2', - 'dump-channels', - 'dump-hashes', - 'E', - 'entry', - 'latest', - 'lib', - 'main-script', - 'N', - 'name', - 'offline', - 'params-file', - 'pi', - 'plugins', - 'poll-interval', - 'pool-size', - 'profile', - 'ps', - 'qs', - 'queue-size', - 'r', - 'resume', - 'revision', - 'stdin', - 'stub', - 'stub-run', - 'test', - 'w', - 'with-charliecloud', - 'with-conda', - 'with-dag', - 'with-docker', - 'with-mpi', - 'with-notification', - 'with-podman', - 'with-report', - 'with-singularity', - 'with-timeline', - 'with-tower', - 'with-trace', - 'with-weblog', - 'without-docker', - 'without-podman', - 'work-dir' - ] - def unexpectedParams = [] - - // Collect expected parameters from the schema - def expectedParams = [] - def enums = [:] - for (group in schemaParams) { - for (p in group.value['properties']) { - expectedParams.push(p.key) - if (group.value['properties'][p.key].containsKey('enum')) { - enums[p.key] = group.value['properties'][p.key]['enum'] - } - } - } - - for (specifiedParam in params.keySet()) { - // nextflow params - if (nf_params.contains(specifiedParam)) { - log.error "ERROR: You used a core Nextflow option with two hyphens: '--${specifiedParam}'. Please resubmit with '-${specifiedParam}'" - has_error = true - } - // unexpected params - def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' - def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } - def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() - def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase)) - if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) { - // Temporarily remove camelCase/camel-case params #1035 - def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} - if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ - unexpectedParams.push(specifiedParam) - } - } - } - - //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~// - // Validate parameters against the schema - InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream() - JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream)) - - // Remove anything that's in params.schema_ignore_params - raw_schema = removeIgnoredParams(raw_schema, params) - - Schema schema = SchemaLoader.load(raw_schema) - - // Clean the parameters - def cleanedParams = cleanParameters(params) - - // Convert to JSONObject - def jsonParams = new JsonBuilder(cleanedParams) - JSONObject params_json = new JSONObject(jsonParams.toString()) - - // Validate - try { - schema.validate(params_json) - } catch (ValidationException e) { - println '' - log.error 'ERROR: Validation of pipeline parameters failed!' - JSONObject exceptionJSON = e.toJSON() - printExceptions(exceptionJSON, params_json, log, enums) - println '' - has_error = true - } - - // Check for unexpected parameters - if (unexpectedParams.size() > 0) { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - println '' - def warn_msg = 'Found unexpected parameters:' - for (unexpectedParam in unexpectedParams) { - warn_msg = warn_msg + "\n* --${unexpectedParam}: ${params[unexpectedParam].toString()}" - } - log.warn warn_msg - log.info "- ${colors.dim}Ignore this warning: params.schema_ignore_params = \"${unexpectedParams.join(',')}\" ${colors.reset}" - println '' - } - - if (has_error) { - System.exit(1) - } - } - - // - // Beautify parameters for --help - // - public static String paramsHelp(workflow, params, command, schema_filename='nextflow_schema.json') { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - Integer num_hidden = 0 - String output = '' - output += 'Typical pipeline command:\n\n' - output += " ${colors.cyan}${command}${colors.reset}\n\n" - Map params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) - Integer max_chars = paramsMaxChars(params_map) + 1 - Integer desc_indent = max_chars + 14 - Integer dec_linewidth = 160 - desc_indent - for (group in params_map.keySet()) { - Integer num_params = 0 - String group_output = colors.underlined + colors.bold + group + colors.reset + '\n' - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (group_params.get(param).hidden && !params.show_hidden_params) { - num_hidden += 1 - continue; - } - def type = '[' + group_params.get(param).type + ']' - def description = group_params.get(param).description - def defaultValue = group_params.get(param).default != null ? " [default: " + group_params.get(param).default.toString() + "]" : '' - def description_default = description + colors.dim + defaultValue + colors.reset - // Wrap long description texts - // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap - if (description_default.length() > dec_linewidth){ - List olines = [] - String oline = "" // " " * indent - description_default.split(" ").each() { wrd -> - if ((oline.size() + wrd.size()) <= dec_linewidth) { - oline += wrd + " " - } else { - olines += oline - oline = wrd + " " - } - } - olines += oline - description_default = olines.join("\n" + " " * desc_indent) - } - group_output += " --" + param.padRight(max_chars) + colors.dim + type.padRight(10) + colors.reset + description_default + '\n' - num_params += 1 - } - group_output += '\n' - if (num_params > 0){ - output += group_output - } - } - if (num_hidden > 0){ - output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset - } - output += NfcoreTemplate.dashedLine(params.monochrome_logs) - return output - } - - // - // Groovy Map summarising parameters/workflow options used by the pipeline - // - public static LinkedHashMap paramsSummaryMap(workflow, params, schema_filename='nextflow_schema.json') { - // Get a selection of core Nextflow workflow options - def Map workflow_summary = [:] - if (workflow.revision) { - workflow_summary['revision'] = workflow.revision - } - workflow_summary['runName'] = workflow.runName - if (workflow.containerEngine) { - workflow_summary['containerEngine'] = workflow.containerEngine - } - if (workflow.container) { - workflow_summary['container'] = workflow.container - } - workflow_summary['launchDir'] = workflow.launchDir - workflow_summary['workDir'] = workflow.workDir - workflow_summary['projectDir'] = workflow.projectDir - workflow_summary['userName'] = workflow.userName - workflow_summary['profile'] = workflow.profile - workflow_summary['configFiles'] = workflow.configFiles.join(', ') - - // Get pipeline parameters defined in JSON Schema - def Map params_summary = [:] - def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) - for (group in params_map.keySet()) { - def sub_params = new LinkedHashMap() - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (params.containsKey(param)) { - def params_value = params.get(param) - def schema_value = group_params.get(param).default - def param_type = group_params.get(param).type - if (schema_value != null) { - if (param_type == 'string') { - if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) { - def sub_string = schema_value.replace('\$projectDir', '') - sub_string = sub_string.replace('\${projectDir}', '') - if (params_value.contains(sub_string)) { - schema_value = params_value - } - } - if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) { - def sub_string = schema_value.replace('\$params.outdir', '') - sub_string = sub_string.replace('\${params.outdir}', '') - if ("${params.outdir}${sub_string}" == params_value) { - schema_value = params_value - } - } - } - } - - // We have a default in the schema, and this isn't it - if (schema_value != null && params_value != schema_value) { - sub_params.put(param, params_value) - } - // No default in the schema, and this isn't empty - else if (schema_value == null && params_value != "" && params_value != null && params_value != false) { - sub_params.put(param, params_value) - } - } - } - params_summary.put(group, sub_params) - } - return [ 'Core Nextflow options' : workflow_summary ] << params_summary - } - - // - // Beautify parameters for summary and return as string - // - public static String paramsSummaryLog(workflow, params) { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - String output = '' - def params_map = paramsSummaryMap(workflow, params) - def max_chars = paramsMaxChars(params_map) - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - if (group_params) { - output += colors.bold + group + colors.reset + '\n' - for (param in group_params.keySet()) { - output += " " + colors.blue + param.padRight(max_chars) + ": " + colors.green + group_params.get(param) + colors.reset + '\n' - } - output += '\n' - } - } - output += "!! Only displaying parameters that differ from the pipeline defaults !!\n" - output += NfcoreTemplate.dashedLine(params.monochrome_logs) - return output - } - - // - // Loop over nested exceptions and print the causingException - // - private static void printExceptions(ex_json, params_json, log, enums, limit=5) { - def causingExceptions = ex_json['causingExceptions'] - if (causingExceptions.length() == 0) { - def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/ - // Missing required param - if (m.matches()) { - log.error "* Missing required parameter: --${m[0][1]}" - } - // Other base-level error - else if (ex_json['pointerToViolation'] == '#') { - log.error "* ${ex_json['message']}" - } - // Error with specific param - else { - def param = ex_json['pointerToViolation'] - ~/^#\// - def param_val = params_json[param].toString() - if (enums.containsKey(param)) { - def error_msg = "* --${param}: '${param_val}' is not a valid choice (Available choices" - if (enums[param].size() > limit) { - log.error "${error_msg} (${limit} of ${enums[param].size()}): ${enums[param][0..limit-1].join(', ')}, ... )" - } else { - log.error "${error_msg}: ${enums[param].join(', ')})" - } - } else { - log.error "* --${param}: ${ex_json['message']} (${param_val})" - } - } - } - for (ex in causingExceptions) { - printExceptions(ex, params_json, log, enums) - } - } - - // - // Remove an element from a JSONArray - // - private static JSONArray removeElement(json_array, element) { - def list = [] - int len = json_array.length() - for (int i=0;i - if(raw_schema.keySet().contains('definitions')){ - raw_schema.definitions.each { definition -> - for (key in definition.keySet()){ - if (definition[key].get("properties").keySet().contains(ignore_param)){ - // Remove the param to ignore - definition[key].get("properties").remove(ignore_param) - // If the param was required, change this - if (definition[key].has("required")) { - def cleaned_required = removeElement(definition[key].required, ignore_param) - definition[key].put("required", cleaned_required) - } - } - } - } - } - if(raw_schema.keySet().contains('properties') && raw_schema.get('properties').keySet().contains(ignore_param)) { - raw_schema.get("properties").remove(ignore_param) - } - if(raw_schema.keySet().contains('required') && raw_schema.required.contains(ignore_param)) { - def cleaned_required = removeElement(raw_schema.required, ignore_param) - raw_schema.put("required", cleaned_required) - } - } - return raw_schema - } - - // - // Clean and check parameters relative to Nextflow native classes - // - private static Map cleanParameters(params) { - def new_params = params.getClass().newInstance(params) - for (p in params) { - // remove anything evaluating to false - if (!p['value']) { - new_params.remove(p.key) - } - // Cast MemoryUnit to String - if (p['value'].getClass() == nextflow.util.MemoryUnit) { - new_params.replace(p.key, p['value'].toString()) - } - // Cast Duration to String - if (p['value'].getClass() == nextflow.util.Duration) { - new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day")) - } - // Cast LinkedHashMap to String - if (p['value'].getClass() == LinkedHashMap) { - new_params.replace(p.key, p['value'].toString()) - } - } - return new_params - } - - // - // This function tries to read a JSON params file - // - private static LinkedHashMap paramsLoad(String json_schema) { - def params_map = new LinkedHashMap() - try { - params_map = paramsRead(json_schema) - } catch (Exception e) { - println "Could not read parameters settings from JSON. $e" - params_map = new LinkedHashMap() - } - return params_map - } - - // - // Method to actually read in JSON file using Groovy. - // Group (as Key), values are all parameters - // - Parameter1 as Key, Description as Value - // - Parameter2 as Key, Description as Value - // .... - // Group - // - - private static LinkedHashMap paramsRead(String json_schema) throws Exception { - def json = new File(json_schema).text - def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') - def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') - /* Tree looks like this in nf-core schema - * definitions <- this is what the first get('definitions') gets us - group 1 - title - description - properties - parameter 1 - type - description - parameter 2 - type - description - group 2 - title - description - properties - parameter 1 - type - description - * properties <- parameters can also be ungrouped, outside of definitions - parameter 1 - type - description - */ - - // Grouped params - def params_map = new LinkedHashMap() - schema_definitions.each { key, val -> - def Map group = schema_definitions."$key".properties // Gets the property object of the group - def title = schema_definitions."$key".title - def sub_params = new LinkedHashMap() - group.each { innerkey, value -> - sub_params.put(innerkey, value) - } - params_map.put(title, sub_params) - } - - // Ungrouped params - def ungrouped_params = new LinkedHashMap() - schema_properties.each { innerkey, value -> - ungrouped_params.put(innerkey, value) - } - params_map.put("Other parameters", ungrouped_params) - - return params_map - } - - // - // Get maximum number of characters across all parameter names - // - private static Integer paramsMaxChars(params_map) { - Integer max_chars = 0 - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (param.size() > max_chars) { - max_chars = param.size() - } - } - } - return max_chars - } -} diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index 4ecac8f..0000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,317 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir" ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.blue} _____ ${colors.green} _______ ${colors.red} _${colors.reset} - ${colors.blue} / ____| ${colors.green}|__ __| ${colors.red}| |${colors.reset} - ${colors.blue} | (___ __ _ _ __ __ _ ___ _ __ ${colors.reset}______${colors.green}| |${colors.yellow} ___ ${colors.red}| |${colors.reset} - ${colors.blue} \\___ \\ / _` | '_ \\ / _` |/ _ \\ '__|${colors.reset}______${colors.green}| |${colors.yellow}/ _ \\${colors.red}| |${colors.reset} - ${colors.blue} ____) | (_| | | | | (_| | __/ | ${colors.green}| |${colors.yellow} (_) ${colors.red}| |____${colors.reset} - ${colors.blue} |_____/ \\__,_|_| |_|\\__, |\\___|_| ${colors.green}|_|${colors.yellow}\\___/${colors.red}}|______|${colors.reset} - ${colors.blue} __/ |${colors.reset} - ${colors.blue} |___/${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100644 index 8d030f4..0000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index 94604a3..0000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,88 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the sanger-tol/readmapping pipeline -// - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - // - // Generate help string - // - public static String help(workflow, params, log) { - def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --fasta reference.fa -profile docker" - def help_string = '' - help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs) - help_string += NfcoreSchema.paramsHelp(workflow, params, command) - help_string += '\n' + citation(workflow) + '\n' - help_string += NfcoreTemplate.dashedLine(params.monochrome_logs) - return help_string - } - - // - // Generate parameter summary log string - // - public static String paramsSummaryLog(workflow, params, log) { - def summary_log = '' - summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs) - summary_log += NfcoreSchema.paramsSummaryLog(workflow, params) - summary_log += '\n' + citation(workflow) + '\n' - summary_log += NfcoreTemplate.dashedLine(params.monochrome_logs) - return summary_log - } - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log) { - // Print help to screen if required - if (params.help) { - log.info help(workflow, params, log) - System.exit(0) - } - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Print parameter summary log to screen - log.info paramsSummaryLog(workflow, params, log) - - // Validate workflow parameters via the JSON schema - if (params.validate_params) { - NfcoreSchema.validateParameters(workflow, params, log) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - - // Check that conda channels are set-up correctly - if (params.enable_conda) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - log.error "Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'" - System.exit(1) - } - } - } diff --git a/lib/WorkflowReadmapping.groovy b/lib/WorkflowReadmapping.groovy deleted file mode 100755 index db87995..0000000 --- a/lib/WorkflowReadmapping.groovy +++ /dev/null @@ -1,21 +0,0 @@ -// -// This file holds several functions specific to the workflow/readmapping.nf in the sanger-tol/readmapping pipeline -// - -import groovy.text.SimpleTemplateEngine - -class WorkflowReadmapping { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - - if (!params.fasta) { - log.error "Genome fasta file not specified with e.g. 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https://github.com/sanger-tol/readmapping + Github : https://github.com/nf-core/readmapping + Website: https://nf-co.re/readmapping + Slack : https://nfcore.slack.com/channels/readmapping ---------------------------------------------------------------------------------------- */ @@ -11,39 +13,96 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE & PRINT PARAMETER SUMMARY + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -WorkflowMain.initialise(workflow, params, log) +include { READMAPPING } from './workflows/readmapping' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_readmapping_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_readmapping_pipeline' + +include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_readmapping_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - NAMED WORKFLOW FOR PIPELINE + GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { READMAPPING } from './workflows/readmapping' +// TODO nf-core: Remove this line if you don't need a FASTA file +// This is an example of how to use getGenomeAttribute() to fetch parameters +// from igenomes.config using `--genome` +params.fasta = getGenomeAttribute('fasta') + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + NAMED WORKFLOWS FOR PIPELINE +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ // -// WORKFLOW: Run main sanger-tol/readmapping analysis pipeline +// WORKFLOW: Run main analysis pipeline depending on type of input // -workflow SANGERTOL_READMAPPING { - READMAPPING () -} +workflow NFCORE_READMAPPING { + + take: + samplesheet // channel: samplesheet read in from --input + + main: + // + // WORKFLOW: Run pipeline + // + READMAPPING ( + samplesheet + ) + + emit: + multiqc_report = READMAPPING.out.multiqc_report // channel: /path/to/multiqc_report.html + +} /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN ALL WORKFLOWS + RUN MAIN WORKFLOW ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// WORKFLOW: Execute a single named workflow for the pipeline -// See: https://github.com/nf-core/rnaseq/issues/619 -// workflow { - SANGERTOL_READMAPPING () + + main: + + // + // SUBWORKFLOW: Run initialisation tasks + // + PIPELINE_INITIALISATION ( + params.version, + params.help, + params.validate_params, + params.monochrome_logs, + args, + params.outdir, + params.input + ) + + // + // WORKFLOW: Run main workflow + // + NFCORE_READMAPPING ( + PIPELINE_INITIALISATION.out.samplesheet + ) + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + NFCORE_READMAPPING.out.multiqc_report + ) } /* diff --git a/modules.json b/modules.json index 260c57c..76be2ac 100644 --- a/modules.json +++ b/modules.json @@ -1,16 +1,38 @@ { - "name": "sanger-tol/readmapping", - "homePage": "https://github.com/sanger-tol/readmapping", + "name": "nf-core/readmapping", + "homePage": "https://github.com/nf-core/readmapping", "repos": { "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "custom/dumpsoftwareversions": { + "fastqc": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", + "installed_by": ["modules"] + }, + "multiqc": { + "branch": "master", + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", + "installed_by": ["modules"] + } + } + }, + "subworkflows": { + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] } } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index a142786..0000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -process SAMPLESHEET_CHECK { - tag "$samplesheet" - label 'process_single' - - conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'quay.io/biocontainers/python:3.8.3' }" - - input: - path samplesheet - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // This script is bundled with the pipeline, in sanger-tol/readmapping/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf deleted file mode 100644 index cebb6e0..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ /dev/null @@ -1,24 +0,0 @@ -process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_single' - - // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda (params.enable_conda ? 'bioconda::multiqc=1.13' : null) - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }" - - input: - path versions - - output: - path "software_versions.yml" , emit: yml - path "software_versions_mqc.yml", emit: mqc_yml - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - template 'dumpsoftwareversions.py' -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml deleted file mode 100644 index 60b546a..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ /dev/null @@ -1,34 +0,0 @@ -name: custom_dumpsoftwareversions -description: Custom module used to dump software versions within the nf-core pipeline template -keywords: - - custom - - version -tools: - - custom: - description: Custom module used to dump software versions within the nf-core pipeline template - homepage: https://github.com/nf-core/tools - documentation: https://github.com/nf-core/tools - licence: ["MIT"] -input: - - versions: - type: file - description: YML file containing software versions - pattern: "*.yml" - -output: - - yml: - type: file - description: Standard YML file containing software versions - pattern: "software_versions.yml" - - mqc_yml: - type: file - description: MultiQC custom content YML file containing software versions - pattern: "software_versions_mqc.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - -authors: - - "@drpatelh" - - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py deleted file mode 100644 index 787bdb7..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ /dev/null @@ -1,91 +0,0 @@ -#!/usr/bin/env python - -import platform -from textwrap import dedent - -import yaml - - -def _make_versions_html(versions): - html = [ - dedent( - """\\ - - - - - - - - - - """ - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f"""\\ - - - - - - """ - ) - ) - html.append("") - html.append("
    Process Name Software Version
    {process if (i == 0) else ''}{tool}{version}
    ") - return "\\n".join(html) - - -versions_this_module = {} -versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, -} - -with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - -# aggregate versions by the module name (derived from fully-qualified process name) -versions_by_module = {} -for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - -versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", -} - -versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), -} - -with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) -with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - -with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml new file mode 100644 index 0000000..1787b38 --- /dev/null +++ b/modules/nf-core/fastqc/environment.yml @@ -0,0 +1,7 @@ +name: fastqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf new file mode 100644 index 0000000..d79f1c8 --- /dev/null +++ b/modules/nf-core/fastqc/main.nf @@ -0,0 +1,61 @@ +process FASTQC { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : + 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.html"), emit: html + tuple val(meta), path("*.zip") , emit: zip + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + // Make list of old name and new name pairs to use for renaming in the bash while loop + def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } + def rename_to = old_new_pairs*.join(' ').join(' ') + def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') + + def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') + // FastQC memory value allowed range (100 - 10000) + def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb) + + """ + printf "%s %s\\n" $rename_to | while read old_name new_name; do + [ -f "\${new_name}" ] || ln -s \$old_name \$new_name + done + + fastqc \\ + $args \\ + --threads $task.cpus \\ + --memory $fastqc_memory \\ + $renamed_files + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.html + touch ${prefix}.zip + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml new file mode 100644 index 0000000..ee5507e --- /dev/null +++ b/modules/nf-core/fastqc/meta.yml @@ -0,0 +1,57 @@ +name: fastqc +description: Run FastQC on sequenced reads +keywords: + - quality control + - qc + - adapters + - fastq +tools: + - fastqc: + description: | + FastQC gives general quality metrics about your reads. + It provides information about the quality score distribution + across your reads, the per base sequence content (%A/C/G/T). + You get information about adapter contamination and other + overrepresented sequences. + homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ + documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ + licence: ["GPL-2.0-only"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - html: + type: file + description: FastQC report + pattern: "*_{fastqc.html}" + - zip: + type: file + description: FastQC report archive + pattern: "*_{fastqc.zip}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" +maintainers: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test new file mode 100644 index 0000000..70edae4 --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -0,0 +1,212 @@ +nextflow_process { + + name "Test Process FASTQC" + script "../main.nf" + process "FASTQC" + + tag "modules" + tag "modules_nfcore" + tag "fastqc" + + test("sarscov2 single-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:

    + // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_single") } + ) + } + } + + test("sarscov2 paired-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } + ) + } + } + + test("sarscov2 interleaved [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } + ) + } + } + + test("sarscov2 paired-end [bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } + ) + } + } + + test("sarscov2 multiple [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][2]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][3]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } + ) + } + } + + test("sarscov2 custom_prefix") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } + ) + } + } + + test("sarscov2 single-end [fastq] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.html.collect { file(it[1]).getName() } + + process.out.zip.collect { file(it[1]).getName() } + + process.out.versions ).match("fastqc_stub") } + ) + } + } + +} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap new file mode 100644 index 0000000..86f7c31 --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -0,0 +1,88 @@ +{ + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { + "content": [ + [ + "test.html", + "test.zip", + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" + }, + "fastqc_versions_custom_prefix": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml new file mode 100644 index 0000000..7834294 --- /dev/null +++ b/modules/nf-core/fastqc/tests/tags.yml @@ -0,0 +1,2 @@ +fastqc: + - modules/nf-core/fastqc/** diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml new file mode 100644 index 0000000..ca39fb6 --- /dev/null +++ b/modules/nf-core/multiqc/environment.yml @@ -0,0 +1,7 @@ +name: multiqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.21 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf new file mode 100644 index 0000000..47ac352 --- /dev/null +++ b/modules/nf-core/multiqc/main.nf @@ -0,0 +1,55 @@ +process MULTIQC { + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : + 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" + + input: + path multiqc_files, stageAs: "?/*" + path(multiqc_config) + path(extra_multiqc_config) + path(multiqc_logo) + + output: + path "*multiqc_report.html", emit: report + path "*_data" , emit: data + path "*_plots" , optional:true, emit: plots + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def config = multiqc_config ? "--config $multiqc_config" : '' + def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' + def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' + """ + multiqc \\ + --force \\ + $args \\ + $config \\ + $extra_config \\ + $logo \\ + . + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) + END_VERSIONS + """ + + stub: + """ + mkdir multiqc_data + touch multiqc_plots + touch multiqc_report.html + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml new file mode 100644 index 0000000..45a9bc3 --- /dev/null +++ b/modules/nf-core/multiqc/meta.yml @@ -0,0 +1,58 @@ +name: multiqc +description: Aggregate results from bioinformatics analyses across many samples into a single report +keywords: + - QC + - bioinformatics tools + - Beautiful stand-alone HTML report +tools: + - multiqc: + description: | + MultiQC searches a given directory for analysis logs and compiles a HTML report. + It's a general use tool, perfect for summarising the output from numerous bioinformatics tools. + homepage: https://multiqc.info/ + documentation: https://multiqc.info/docs/ + licence: ["GPL-3.0-or-later"] +input: + - multiqc_files: + type: file + description: | + List of reports / files recognised by MultiQC, for example the html and zip output of FastQC + - multiqc_config: + type: file + description: Optional config yml for MultiQC + pattern: "*.{yml,yaml}" + - extra_multiqc_config: + type: file + description: Second optional config yml for MultiQC. Will override common sections in multiqc_config. + pattern: "*.{yml,yaml}" + - multiqc_logo: + type: file + description: Optional logo file for MultiQC + pattern: "*.{png}" +output: + - report: + type: file + description: MultiQC report file + pattern: "multiqc_report.html" + - data: + type: directory + description: MultiQC data dir + pattern: "multiqc_data" + - plots: + type: file + description: Plots created by MultiQC + pattern: "*_data" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@abhi18av" + - "@bunop" + - "@drpatelh" + - "@jfy133" +maintainers: + - "@abhi18av" + - "@bunop" + - "@drpatelh" + - "@jfy133" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test new file mode 100644 index 0000000..f1c4242 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -0,0 +1,84 @@ +nextflow_process { + + name "Test Process MULTIQC" + script "../main.nf" + process "MULTIQC" + + tag "modules" + tag "modules_nfcore" + tag "multiqc" + + test("sarscov2 single-end [fastqc]") { + + when { + process { + """ + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("multiqc_versions_single") } + ) + } + + } + + test("sarscov2 single-end [fastqc] [config]") { + + when { + process { + """ + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) + input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("multiqc_versions_config") } + ) + } + } + + test("sarscov2 single-end [fastqc] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.report.collect { file(it).getName() } + + process.out.data.collect { file(it).getName() } + + process.out.plots.collect { file(it).getName() } + + process.out.versions ).match("multiqc_stub") } + ) + } + + } +} diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap new file mode 100644 index 0000000..bfebd80 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -0,0 +1,41 @@ +{ + "multiqc_versions_single": { + "content": [ + [ + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T08:48:55.657331" + }, + "multiqc_stub": { + "content": [ + [ + "multiqc_report.html", + "multiqc_data", + "multiqc_plots", + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T08:49:49.071937" + }, + "multiqc_versions_config": { + "content": [ + [ + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T08:49:25.457567" + } +} \ No newline at end of file diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml new file mode 100644 index 0000000..bea6c0d --- /dev/null +++ b/modules/nf-core/multiqc/tests/tags.yml @@ -0,0 +1,2 @@ +multiqc: + - modules/nf-core/multiqc/** diff --git a/nextflow.config b/nextflow.config index b00a2eb..6ca68d7 100644 --- a/nextflow.config +++ b/nextflow.config @@ -1,6 +1,6 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - sanger-tol/readmapping Nextflow config file + nf-core/readmapping Nextflow config file ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Default config options for all compute environments ---------------------------------------------------------------------------------------- @@ -12,32 +12,37 @@ params { // TODO nf-core: Specify your pipeline's command line flags // Input options input = null + // References + genome = null + igenomes_base = 's3://ngi-igenomes/igenomes/' + igenomes_ignore = false - // Boilerplate options - outdir = null - tracedir = "${params.outdir}/pipeline_info" - publish_dir_mode = 'copy' - email = null - email_on_fail = null - plaintext_email = false - monochrome_logs = false - hook_url = null - help = false - version = false - validate_params = true - show_hidden_params = false - schema_ignore_params = 'genomes' - enable_conda = false + // MultiQC options + multiqc_config = null + multiqc_title = null + multiqc_logo = null + max_multiqc_email_size = '25.MB' + multiqc_methods_description = null + // Boilerplate options + outdir = null + publish_dir_mode = 'copy' + email = null + email_on_fail = null + plaintext_email = false + monochrome_logs = false + hook_url = null + help = false + version = false + pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' // Config options + config_profile_name = null + config_profile_description = null custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" - config_profile_description = null config_profile_contact = null config_profile_url = null - config_profile_name = null - // Max resource options // Defaults only, expecting to be overwritten @@ -45,6 +50,13 @@ params { max_cpus = 16 max_time = '240.h' + // Schema validation default options + validationFailUnrecognisedParams = false + validationLenientMode = false + validationSchemaIgnoreParams = 'genomes,igenomes_base' + validationShowHiddenParams = false + validate_params = true + } // Load base.config by default for all pipelines @@ -57,87 +69,134 @@ try { System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") } -// Load sanger-tol/readmapping custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs! -// try { -// includeConfig "${params.custom_config_base}/pipeline/readmapping.config" -// } catch (Exception e) { -// System.err.println("WARNING: Could not load nf-core/config/readmapping profiles: ${params.custom_config_base}/pipeline/readmapping.config") -// } - - +// Load nf-core/readmapping custom profiles from different institutions. +try { + includeConfig "${params.custom_config_base}/pipeline/readmapping.config" +} catch (Exception e) { + System.err.println("WARNING: Could not load nf-core/config/readmapping profiles: ${params.custom_config_base}/pipeline/readmapping.config") +} profiles { - debug { process.beforeScript = 'echo $HOSTNAME' } + debug { + dumpHashes = true + process.beforeScript = 'echo $HOSTNAME' + cleanup = false + nextflow.enable.configProcessNamesValidation = true + } conda { - params.enable_conda = true - conda.enabled = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false + conda.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + conda.channels = ['conda-forge', 'bioconda', 'defaults'] + apptainer.enabled = false } mamba { - params.enable_conda = true - conda.enabled = true - conda.useMamba = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false + conda.enabled = true + conda.useMamba = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } docker { - docker.enabled = true - docker.userEmulation = true - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false + docker.enabled = true + conda.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { - singularity.enabled = true - singularity.autoMounts = true - docker.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false + singularity.enabled = true + singularity.autoMounts = true + conda.enabled = false + docker.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } podman { - podman.enabled = true - docker.enabled = false - singularity.enabled = false - shifter.enabled = false - charliecloud.enabled = false + podman.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } shifter { - shifter.enabled = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - charliecloud.enabled = false + shifter.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } charliecloud { - charliecloud.enabled = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false + charliecloud.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + apptainer.enabled = false + } + apptainer { + apptainer.enabled = true + apptainer.autoMounts = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + wave { + apptainer.ociAutoPull = true + singularity.ociAutoPull = true + wave.enabled = true + wave.freeze = true + wave.strategy = 'conda,container' } gitpod { - executor.name = 'local' - executor.cpus = 16 - executor.memory = 60.GB + executor.name = 'local' + executor.cpus = 4 + executor.memory = 8.GB } test { includeConfig 'conf/test.config' } test_full { includeConfig 'conf/test_full.config' } } +// Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile +// Will not be used unless Apptainer / Docker / Podman / Singularity are enabled +// Set to your registry if you have a mirror of containers +apptainer.registry = 'quay.io' +docker.registry = 'quay.io' +podman.registry = 'quay.io' +singularity.registry = 'quay.io' +// Nextflow plugins +plugins { + id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet +} +// Load igenomes.config if required +if (!params.igenomes_ignore) { + includeConfig 'conf/igenomes.config' +} else { + params.genomes = [:] +} // Export these variables to prevent local Python/R libraries from conflicting with those in the container // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container. // See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable. @@ -152,32 +211,35 @@ env { // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] +// Disable process selector warnings by default. Use debug profile to enable warnings. +nextflow.enable.configProcessNamesValidation = false + def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true - file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" + file = "${params.outdir}/pipeline_info/execution_timeline_${trace_timestamp}.html" } report { enabled = true - file = "${params.tracedir}/execution_report_${trace_timestamp}.html" + file = "${params.outdir}/pipeline_info/execution_report_${trace_timestamp}.html" } trace { enabled = true - file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" + file = "${params.outdir}/pipeline_info/execution_trace_${trace_timestamp}.txt" } dag { enabled = true - file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.html" + file = "${params.outdir}/pipeline_info/pipeline_dag_${trace_timestamp}.html" } manifest { - name = 'sanger-tol/readmapping' + name = 'nf-core/readmapping' author = """@priyanka-surana""" - homePage = 'https://github.com/sanger-tol/readmapping' - description = """map reads to genome""" + homePage = 'https://github.com/nf-core/readmapping' + description = """Pipeline to map reads generated using different sequencing technologies against a genome assembly.""" mainScript = 'main.nf' - nextflowVersion = '!>=22.10.1' - version = '1.1' + nextflowVersion = '!>=23.04.0' + version = '1.3.0' doi = '' } diff --git a/nextflow_schema.json b/nextflow_schema.json index c9f1cf0..623b1a2 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,8 +1,8 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/sanger-tol/readmapping/master/nextflow_schema.json", - "title": "sanger-tol/readmapping pipeline parameters", - "description": "map reads to genome", + "$id": "https://raw.githubusercontent.com/nf-core/readmapping/master/nextflow_schema.json", + "title": "nf-core/readmapping pipeline parameters", + "description": "Pipeline to map reads generated using different sequencing technologies against a genome assembly.", "type": "object", "definitions": { "input_output_options": { @@ -10,19 +10,17 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input", - "outdir" - ], + "required": ["input", "outdir"], "properties": { "input": { "type": "string", "format": "file-path", + "exists": true, + "schema": "assets/schema_input.json", "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", - "schema": "assets/schema_input.json", "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row.", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/readmapping/usage#samplesheet-input).", "fa_icon": "fas fa-file-csv" }, "outdir": { @@ -37,6 +35,11 @@ "fa_icon": "fas fa-envelope", "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + }, + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" } } }, @@ -45,7 +48,31 @@ "type": "object", "fa_icon": "fas fa-dna", "description": "Reference genome related files and options required for the workflow.", - "properties": {} + "properties": { + "genome": { + "type": "string", + "description": "Name of iGenomes reference.", + "fa_icon": "fas fa-book", + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + }, + "fasta": { + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/plain", + "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", + "description": "Path to FASTA genome file.", + "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", + "fa_icon": "far fa-file-code" + }, + "igenomes_ignore": { + "type": "boolean", + "description": "Do not load the iGenomes reference config.", + "fa_icon": "fas fa-ban", + "hidden": true, + "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + } + } }, "institutional_config_options": { "title": "Institutional config options", @@ -124,7 +151,7 @@ "description": "Maximum amount of time that can be requested for any single job.", "default": "240.h", "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", + "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", "hidden": true, "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" } @@ -155,14 +182,7 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { @@ -179,6 +199,14 @@ "fa_icon": "fas fa-remove-format", "hidden": true }, + "max_multiqc_email_size": { + "type": "string", + "description": "File size limit when attaching MultiQC reports to summary emails.", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true + }, "monochrome_logs": { "type": "boolean", "description": "Do not use coloured log outputs.", @@ -192,13 +220,24 @@ "help_text": "Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.", "hidden": true }, - "tracedir": { + "multiqc_config": { + "type": "string", + "format": "file-path", + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true + }, + "multiqc_logo": { "type": "string", - "description": "Directory to keep pipeline Nextflow logs and reports.", - "default": "${params.outdir}/pipeline_info", - "fa_icon": "fas fa-cogs", + "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", + "fa_icon": "fas fa-image", "hidden": true }, + "multiqc_methods_description": { + "type": "string", + "description": "Custom MultiQC yaml file containing HTML including a methods description.", + "fa_icon": "fas fa-cog" + }, "validate_params": { "type": "boolean", "description": "Boolean whether to validate parameters against the schema at runtime", @@ -206,18 +245,33 @@ "fa_icon": "fas fa-check-square", "hidden": true }, - "show_hidden_params": { + "validationShowHiddenParams": { "type": "boolean", "fa_icon": "far fa-eye-slash", "description": "Show all params when using `--help`", "hidden": true, "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." }, - "enable_conda": { + "validationFailUnrecognisedParams": { + "type": "boolean", + "fa_icon": "far fa-check-circle", + "description": "Validation of parameters fails when an unrecognised parameter is found.", + "hidden": true, + "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." + }, + "validationLenientMode": { "type": "boolean", - "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", + "fa_icon": "far fa-check-circle", + "description": "Validation of parameters in lenient more.", "hidden": true, - "fa_icon": "fas fa-bacon" + "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." + }, + "pipelines_testdata_base_path": { + "type": "string", + "fa_icon": "far fa-check-circle", + "description": "Base URL or local path to location of pipeline test dataset files", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/", + "hidden": true } } } diff --git a/pyproject.toml b/pyproject.toml deleted file mode 100644 index 0d62beb..0000000 --- a/pyproject.toml +++ /dev/null @@ -1,10 +0,0 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. -# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] -line-length = 120 -target_version = ["py37", "py38", "py39", "py310"] - -[tool.isort] -profile = "black" -known_first_party = ["nf_core"] -multi_line_output = 3 diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf deleted file mode 100644 index 0aecf87..0000000 --- a/subworkflows/local/input_check.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// Check input samplesheet and get read channels -// - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta -} diff --git a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf new file mode 100644 index 0000000..34162fe --- /dev/null +++ b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf @@ -0,0 +1,263 @@ +// +// Subworkflow with functionality specific to the nf-core/readmapping pipeline +// + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { fromSamplesheet } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir // string: The output directory where the results will be saved + input // string: Path to input samplesheet + + main: + + ch_versions = Channel.empty() + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = nfCoreLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + // + // Custom validation for pipeline parameters + // + validateInputParameters() + + // + // Create channel from input file provided through params.input + // + Channel + .fromSamplesheet("input") + .map { + meta, fastq_1, fastq_2 -> + if (!fastq_2) { + return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] + } else { + return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] + } + } + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .map { + meta, fastqs -> + return [ meta, fastqs.flatten() ] + } + .set { ch_samplesheet } + + emit: + samplesheet = ch_samplesheet + versions = ch_versions +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } + + workflow.onError { + log.error "Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting" + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ +// +// Check and validate pipeline parameters +// +def validateInputParameters() { + genomeExistsError() +} + +// +// Validate channels from input samplesheet +// +def validateInputSamplesheet(input) { + def (metas, fastqs) = input[1..2] + + // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + if (!endedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") + } + + return [ metas[0], fastqs ] +} +// +// Get attribute from genome config file e.g. fasta +// +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} + +// +// Exit pipeline if incorrect --genome key provided +// +def genomeExistsError() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } +} + +// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
  • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
  • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
  • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + if (meta.manifest_map.doi) { + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + def temp_doi_ref = "" + String[] manifest_doi = meta.manifest_map.doi.tokenize(",") + for (String doi_ref: manifest_doi) temp_doi_ref += "(doi:
    ${doi_ref.replace("https://doi.org/", "").replace(" ", "")}), " + meta["doi_text"] = temp_doi_ref.substring(0, temp_doi_ref.length() - 2) + } else meta["doi_text"] = "" + meta["nodoi_text"] = meta.manifest_map.doi ? "" : "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
  • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + + + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 0000000..ac31f28 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 0000000..e5c3a0a --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 0000000..68718e4 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 0000000..e3f0baf --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,20 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:05.308243" + }, + "Test Function checkCondaChannels": { + "content": null, + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:12.425833" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 0000000..ca964ce --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,111 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + workflow { + """ + print_version = false + dump_parameters = true + outdir = 'results' + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + workflow { + """ + print_version = false + dump_parameters = true + outdir = null + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 0000000..d0a926b --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 0000000..f847611 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 0000000..14558c3 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,446 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + def temp_doi_ref = "" + String[] manifest_doi = workflow.manifest.doi.tokenize(",") + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + for (String doi_ref: manifest_doi) temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + temp_doi_ref + "\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 0000000..d08d243 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 0000000..1dc317f --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 0000000..1037232 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,166 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:03.360873" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:59.729647" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:10.562934" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:07.019761" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:17.969323" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:14.366181" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:21.714424" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 0000000..8940d32 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 0000000..859d103 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,19 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:25.726491" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 0000000..d0a926b --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 0000000..ac8523c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 0000000..2585b65 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 0000000..3d4a6b0 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 0000000..5784a33 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 0000000..7626c1c --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 0000000..60b1cff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/tower.yml b/tower.yml new file mode 100644 index 0000000..787aedf --- /dev/null +++ b/tower.yml @@ -0,0 +1,5 @@ +reports: + multiqc_report.html: + display: "MultiQC HTML report" + samplesheet.csv: + display: "Auto-created samplesheet with collated metadata and FASTQ paths" diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index 3174b60..de0111e 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -1,50 +1,15 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE INPUTS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) - -// Validate input parameters -WorkflowReadmapping.initialise(params, log) - -// TODO nf-core: Add all file path parameters for the pipeline to the list below -// Check input path parameters to see if they exist -def checkPathParamList = [ params.input, params.fasta ] -for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } - -// Check mandatory parameters -if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES + IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT LOCAL MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT NF-CORE MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// MODULE: Installed directly from nf-core/modules -// -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_readmapping_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -54,39 +19,76 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft workflow READMAPPING { + take: + ch_samplesheet // channel: samplesheet read in from --input + + main: + ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files + // MODULE: Run FastQC // - INPUT_CHECK ( - ch_input + FASTQC ( + ch_samplesheet ) - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) + ch_versions = ch_versions.mix(FASTQC.out.versions.first()) // - // MODULE: Combine different versions.yml + // Collate and save software versions // - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique{ it.text }.collectFile(name: 'collated_versions.yml') - ) + softwareVersionsToYAML(ch_versions) + .collectFile( + storeDir: "${params.outdir}/pipeline_info", + name: 'nf_core_pipeline_software_mqc_versions.yml', + sort: true, + newLine: true + ).set { ch_collated_versions } -} + // + // MODULE: MultiQC + // + ch_multiqc_config = Channel.fromPath( + "$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? + Channel.fromPath(params.multiqc_config, checkIfExists: true) : + Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? + Channel.fromPath(params.multiqc_logo, checkIfExists: true) : + Channel.empty() + + summary_params = paramsSummaryMap( + workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? + file(params.multiqc_methods_description, checkIfExists: true) : + file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value( + methodsDescriptionText(ch_multiqc_custom_methods_description)) + + ch_multiqc_files = ch_multiqc_files.mix( + ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix( + ch_methods_description.collectFile( + name: 'methods_description_mqc.yaml', + sort: true + ) + ) -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ + MULTIQC ( + ch_multiqc_files.collect(), + ch_multiqc_config.toList(), + ch_multiqc_custom_config.toList(), + ch_multiqc_logo.toList() + ) -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log) - } - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) - } + emit: + multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [ path(versions.yml) ] } /* From 34731f848296dbc37527479f1305401dc5bf1ef1 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 19 Jul 2024 13:35:06 +0100 Subject: [PATCH 062/111] revert nf-core sync changes --- README.md | 33 ++++++++++++++++----------------- 1 file changed, 16 insertions(+), 17 deletions(-) diff --git a/README.md b/README.md index 2a3b8e7..c289d56 100644 --- a/README.md +++ b/README.md @@ -2,13 +2,11 @@ [![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.6563577-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.6563577) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.10.1-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/readmapping) - -[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23readmapping-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/readmapping)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) +[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/sanger-tol/readmapping) ## Introduction @@ -22,24 +20,24 @@ On merge to `dev` and `main` branch, automated continuous integration tests run -> [!NOTE] -> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. +## Quick Start - + > - The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`. + > - Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. + > - If you are using `singularity`, please use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs. + > - If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs. 4. Start running your own analysis! @@ -55,6 +53,7 @@ We thank the following people for their extensive assistance in the development - [Matthieu Muffato](https://github.com/muffato) for the text logo - [Guoying Qi](https://github.com/gq1) for being able to run tests using Nf-Tower and the Sanger HPC farm +- [Tyler Chafin](https://github.com/tkchafin) for maintenance and updates ## Contributions and Support @@ -68,7 +67,7 @@ If you use sanger-tol/readmapping for your analysis, please cite it using the fo An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. -You can cite the `nf-core` publication as follows: +This pipeline uses code and infrastructure developed and maintained by the [nf-core](https://nf-co.re) community, reused here under the [MIT license](https://github.com/nf-core/tools/blob/master/LICENSE). > **The nf-core framework for community-curated bioinformatics pipelines.** > From fe5074a06df31110545ce56912d3f27304817ddd Mon Sep 17 00:00:00 2001 From: reichan1998 Date: Mon, 22 Jul 2024 18:10:23 +0700 Subject: [PATCH 063/111] add condition check to disable bwamem2 index if align_short will not be run --- subworkflows/local/prepare_genome.nf | 35 ++++++++++++++++------------ workflows/readmapping.nf | 13 +++++++++-- 2 files changed, 31 insertions(+), 17 deletions(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 5264569..0fe5a8b 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -11,7 +11,7 @@ include { BWAMEM2_INDEX } from '../../modules/nf-core/bwamem2/index/main' workflow PREPARE_GENOME { take: fasta // channel: [ meta, /path/to/fasta ] - + short_reads // channel: [ meta, /path/to/reads ] main: ch_versions = Channel.empty() @@ -33,24 +33,29 @@ workflow PREPARE_GENOME { UNMASK ( ch_fasta ) ch_versions = ch_versions.mix ( UNMASK.out.versions ) + // def align_short = short_reads ? true : false // Generate BWA index - if ( params.bwamem2_index ) { - Channel.fromPath ( params.bwamem2_index ) - | combine ( ch_fasta ) - | map { bwa, meta, fa -> [ meta, bwa ] } - | set { ch_bwamem } - - if ( params.bwamem2_index.endsWith('.tar.gz') ) { - ch_bwamem2_index = UNTAR ( ch_bwamem ).untar - ch_versions = ch_versions.mix ( UNTAR.out.versions ) + ch_bwamem2_index = Channel.empty() + + if ( short_reads ) { + if ( params.bwamem2_index ) { + Channel.fromPath ( params.bwamem2_index ) + | combine ( ch_fasta ) + | map { bwa, meta, fa -> [ meta, bwa ] } + | set { ch_bwamem } + + if ( params.bwamem2_index.endsWith('.tar.gz') ) { + ch_bwamem2_index = UNTAR ( ch_bwamem ).untar + ch_versions = ch_versions.mix ( UNTAR.out.versions ) + } else { + ch_bwamem2_index = ch_bwamem + } + } else { - ch_bwamem2_index = ch_bwamem + ch_bwamem2_index = BWAMEM2_INDEX ( UNMASK.out.fasta ).index + ch_versions = ch_versions.mix ( BWAMEM2_INDEX.out.versions ) } - - } else { - ch_bwamem2_index = BWAMEM2_INDEX ( UNMASK.out.fasta ).index - ch_versions = ch_versions.mix ( BWAMEM2_INDEX.out.versions ) } diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index 9d12b0b..043c745 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -75,7 +75,16 @@ workflow READMAPPING { | set { ch_reads } ch_versions = ch_versions.mix ( INPUT_CHECK.out.versions ) - + // Determine datatype of short and long reads + INPUT_CHECK.out.reads + | branch { + meta, reads -> + short_reads: meta.datatype == "illumina" || meta.datatype == "hic" + return [meta.datatype, true] + long_reads: meta.datatype == "pacbio" || meta.datatype == "pacbio_clr" || meta.datatype == "ont" + return [meta.datatype, false] + } + | set { ch_filtered_reads } // // SUBWORKFLOW: Uncompress and prepare reference genome files @@ -84,7 +93,7 @@ workflow READMAPPING { | map { [ [ id: it.baseName ], it ] } | set { ch_genome } - PREPARE_GENOME ( ch_genome ) + PREPARE_GENOME ( ch_genome, ch_filtered_reads.short_reads ) ch_versions = ch_versions.mix ( PREPARE_GENOME.out.versions ) From b57595f5db4e01a7106c9410288fd44602be6c3f Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 12:52:22 +0100 Subject: [PATCH 064/111] Restore .github directory from dev branch --- .github/CONTRIBUTING.md | 34 +++----- .github/ISSUE_TEMPLATE/bug_report.yml | 41 +++++----- .github/ISSUE_TEMPLATE/config.yml | 7 -- .github/ISSUE_TEMPLATE/feature_request.yml | 2 +- .github/PULL_REQUEST_TEMPLATE.md | 10 +-- .github/workflows/awsfulltest.yml | 39 ---------- .github/workflows/awstest.yml | 33 -------- .github/workflows/branch.yml | 6 +- .github/workflows/ci.yml | 11 +-- .github/workflows/clean-up.yml | 24 ------ .github/workflows/download_pipeline.yml | 86 --------------------- .github/workflows/fix-linting.yml | 31 +------- .github/workflows/linting.yml | 68 ++++++++++++---- .github/workflows/linting_comment.yml | 6 +- .github/workflows/release-announcements.yml | 75 ------------------ 15 files changed, 104 insertions(+), 369 deletions(-) delete mode 100644 .github/ISSUE_TEMPLATE/config.yml delete mode 100644 .github/workflows/awsfulltest.yml delete mode 100644 .github/workflows/awstest.yml delete mode 100644 .github/workflows/clean-up.yml delete mode 100644 .github/workflows/download_pipeline.yml delete mode 100644 .github/workflows/release-announcements.yml diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 33d301a..2999020 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -1,23 +1,20 @@ -# nf-core/readmapping: Contributing Guidelines +# sanger-tol/readmapping: Contributing Guidelines Hi there! -Many thanks for taking an interest in improving nf-core/readmapping. +Many thanks for taking an interest in improving sanger-tol/readmapping. -We try to manage the required tasks for nf-core/readmapping using GitHub issues, you probably came to this page when creating one. +We try to manage the required tasks for sanger-tol/readmapping using GitHub issues, you probably came to this page when creating one. Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -> [!NOTE] -> If you need help using or modifying nf-core/readmapping then the best place to ask is on the nf-core Slack [#readmapping](https://nfcore.slack.com/channels/readmapping) channel ([join our Slack here](https://nf-co.re/join/slack)). - ## Contribution workflow -If you'd like to write some code for nf-core/readmapping, the standard workflow is as follows: +If you'd like to write some code for sanger-tol/readmapping, the standard workflow is as follows: -1. Check that there isn't already an issue about your idea in the [nf-core/readmapping issues](https://github.com/nf-core/readmapping/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this -2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/readmapping repository](https://github.com/nf-core/readmapping) to your GitHub account +1. Check that there isn't already an issue about your idea in the [sanger-tol/readmapping issues](https://github.com/sanger-tol/readmapping/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this +2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [sanger-tol/readmapping repository](https://github.com/sanger-tol/readmapping) to your GitHub account 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions) 4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). 5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged @@ -26,12 +23,6 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests -You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: - -```bash -nf-test test --profile debug,test,docker --verbose -``` - When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -59,13 +50,9 @@ These tests are run both with the latest available version of `Nextflow` and als - Fix the bug, and bump version (X.Y.Z+1). - A PR should be made on `master` from patch to directly this particular bug. -## Getting help - -For further information/help, please consult the [nf-core/readmapping documentation](https://nf-co.re/readmapping/usage) and don't hesitate to get in touch on the nf-core Slack [#readmapping](https://nfcore.slack.com/channels/readmapping) channel ([join our Slack here](https://nf-co.re/join/slack)). - ## Pipeline contribution conventions -To make the nf-core/readmapping code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written. +To make the sanger-tol/readmapping code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written. ### Adding a new step @@ -79,8 +66,6 @@ If you wish to contribute a new step, please use the following coding standards: 6. Add sanity checks and validation for all relevant parameters. 7. Perform local tests to validate that the new code works as expected. 8. If applicable, add a new test command in `.github/workflow/ci.yml`. -9. Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module. -10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`. ### Default values @@ -92,7 +77,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. ### Naming schemes @@ -115,7 +100,7 @@ This repo includes a devcontainer configuration which will create a GitHub Codes To get started: -- Open the repo in [Codespaces](https://github.com/nf-core/readmapping/codespaces) +- Open the repo in [Codespaces](https://github.com/sanger-tol/readmapping/codespaces) - Tools installed - nf-core - Nextflow @@ -123,3 +108,4 @@ To get started: Devcontainer specs: - [DevContainer config](.devcontainer/devcontainer.json) +- [Dockerfile](.devcontainer/Dockerfile) diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index 2b5a1e1..34acefd 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -2,14 +2,6 @@ name: Bug report description: Report something that is broken or incorrect labels: bug body: - - type: markdown - attributes: - value: | - Before you post this issue, please check the documentation: - - - [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting) - - [nf-core/readmapping pipeline documentation](https://nf-co.re/readmapping/usage) - - type: textarea id: description attributes: @@ -17,34 +9,45 @@ body: description: A clear and concise description of what the bug is. validations: required: true - - type: textarea id: command_used attributes: label: Command used and terminal output - description: Steps to reproduce the behaviour. Please paste the command you used to launch the pipeline and the output from your terminal. + description: Steps to reproduce the behaviour. Please paste the command you used + to launch the pipeline and the output from your terminal. render: console - placeholder: | - $ nextflow run ... + placeholder: "$ nextflow run ... + Some output where something broke + " - type: textarea id: files attributes: label: Relevant files - description: | - Please drag and drop the relevant files here. Create a `.zip` archive if the extension is not allowed. - Your verbose log file `.nextflow.log` is often useful _(this is a hidden file in the directory where you launched the pipeline)_ as well as custom Nextflow configuration files. + description: "Please drag and drop the relevant files here. Create a `.zip` archive + if the extension is not allowed. + + Your verbose log file `.nextflow.log` is often useful _(this is a hidden file + in the directory where you launched the pipeline)_ as well as custom Nextflow + configuration files. + " - type: textarea id: system attributes: label: System information - description: | - * Nextflow version _(eg. 23.04.0)_ + description: "* Nextflow version _(eg. 22.10.1)_ + * Hardware _(eg. HPC, Desktop, Cloud)_ + * Executor _(eg. slurm, local, awsbatch)_ - * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, or Apptainer)_ + + * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_ + * OS _(eg. CentOS Linux, macOS, Linux Mint)_ - * Version of nf-core/readmapping _(eg. 1.1, 1.5, 1.8.2)_ + + * Version of sanger-tol/readmapping _(eg. 1.1, 1.5, 1.8.2)_ + + " diff --git a/.github/ISSUE_TEMPLATE/config.yml b/.github/ISSUE_TEMPLATE/config.yml deleted file mode 100644 index 68bef12..0000000 --- a/.github/ISSUE_TEMPLATE/config.yml +++ /dev/null @@ -1,7 +0,0 @@ -contact_links: - - name: Join nf-core - url: https://nf-co.re/join - about: Please join the nf-core community here - - name: "Slack #readmapping channel" - url: https://nfcore.slack.com/channels/readmapping - about: Discussion about the nf-core/readmapping pipeline diff --git a/.github/ISSUE_TEMPLATE/feature_request.yml b/.github/ISSUE_TEMPLATE/feature_request.yml index e787922..f08da27 100644 --- a/.github/ISSUE_TEMPLATE/feature_request.yml +++ b/.github/ISSUE_TEMPLATE/feature_request.yml @@ -1,5 +1,5 @@ name: Feature request -description: Suggest an idea for the nf-core/readmapping pipeline +description: Suggest an idea for the sanger-tol/readmapping pipeline labels: enhancement body: - type: textarea diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 0a7bfb4..af196cc 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -1,25 +1,23 @@ ## PR checklist - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! -- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/readmapping/tree/master/.github/CONTRIBUTING.md) -- [ ] If necessary, also make a PR on the nf-core/readmapping _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. +- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/sanger-tol/readmapping/tree/master/.github/CONTRIBUTING.md) - [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). -- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. - [ ] `CHANGELOG.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml deleted file mode 100644 index 9bc2cb5..0000000 --- a/.github/workflows/awsfulltest.yml +++ /dev/null @@ -1,39 +0,0 @@ -name: nf-core AWS full size tests -# This workflow is triggered on published releases. -# It can be additionally triggered manually with GitHub actions workflow dispatch button. -# It runs the -profile 'test_full' on AWS batch - -on: - release: - types: [published] - workflow_dispatch: -jobs: - run-platform: - name: Run AWS full tests - if: github.repository == 'nf-core/readmapping' - runs-on: ubuntu-latest - steps: - - name: Launch workflow via Seqera Platform - uses: seqeralabs/action-tower-launch@v2 - # TODO nf-core: You can customise AWS full pipeline tests as required - # Add full size test data (but still relatively small datasets for few samples) - # on the `test_full.config` test runs with only one set of parameters - with: - workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} - access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} - compute_env: ${{ secrets.TOWER_COMPUTE_ENV }} - revision: ${{ github.sha }} - workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/readmapping/work-${{ github.sha }} - parameters: | - { - "hook_url": "${{ secrets.MEGATESTS_ALERTS_SLACK_HOOK_URL }}", - "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/readmapping/results-${{ github.sha }}" - } - profiles: test_full - - - uses: actions/upload-artifact@v4 - with: - name: Seqera Platform debug log file - path: | - seqera_platform_action_*.log - seqera_platform_action_*.json diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml deleted file mode 100644 index 6ec1cc3..0000000 --- a/.github/workflows/awstest.yml +++ /dev/null @@ -1,33 +0,0 @@ -name: nf-core AWS test -# This workflow can be triggered manually with the GitHub actions workflow dispatch button. -# It runs the -profile 'test' on AWS batch - -on: - workflow_dispatch: -jobs: - run-platform: - name: Run AWS tests - if: github.repository == 'nf-core/readmapping' - runs-on: ubuntu-latest - steps: - # Launch workflow using Seqera Platform CLI tool action - - name: Launch workflow via Seqera Platform - uses: seqeralabs/action-tower-launch@v2 - with: - workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} - access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} - compute_env: ${{ secrets.TOWER_COMPUTE_ENV }} - revision: ${{ github.sha }} - workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/readmapping/work-${{ github.sha }} - parameters: | - { - "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/readmapping/results-test-${{ github.sha }}" - } - profiles: test - - - uses: actions/upload-artifact@v4 - with: - name: Seqera Platform debug log file - path: | - seqera_platform_action_*.log - seqera_platform_action_*.json diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 47ffcaa..3a6b782 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -11,15 +11,15 @@ jobs: steps: # PRs to the nf-core repo main branch are only ok if coming from the nf-core repo `dev` or any `patch` branches - name: Check PRs - if: github.repository == 'nf-core/readmapping' + if: github.repository == 'sanger-tol/readmapping' run: | - { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/readmapping ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] + { [[ ${{github.event.pull_request.head.repo.full_name }} == sanger-tol/readmapping ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] # If the above check failed, post a comment on the PR explaining the failure # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 + uses: mshick/add-pr-comment@v1 with: message: | ## This PR is against the `main` branch :x: diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 3364a71..124eab4 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -19,25 +19,22 @@ jobs: test: name: Run pipeline with test data # Only run on push if this is the nf-core dev branch (merged PRs) - if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/readmapping') }}" + if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'sanger-tol/readmapping') }}" runs-on: ubuntu-latest strategy: matrix: NXF_VER: - - "23.04.0" + - "22.10.1" - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + uses: actions/checkout@v3 - name: Install Nextflow - uses: nf-core/setup-nextflow@v2 + uses: nf-core/setup-nextflow@v1 with: version: "${{ matrix.NXF_VER }}" - - name: Disk space cleanup - uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 - - name: Run pipeline with test data # You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml deleted file mode 100644 index 0b6b1f2..0000000 --- a/.github/workflows/clean-up.yml +++ /dev/null @@ -1,24 +0,0 @@ -name: "Close user-tagged issues and PRs" -on: - schedule: - - cron: "0 0 * * 0" # Once a week - -jobs: - clean-up: - runs-on: ubuntu-latest - permissions: - issues: write - pull-requests: write - steps: - - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 - with: - stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." - stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." - close-issue-message: "This issue was closed because it has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor and then staled for 20 days with no activity." - days-before-stale: 30 - days-before-close: 20 - days-before-pr-close: -1 - any-of-labels: "awaiting-changes,awaiting-feedback" - exempt-issue-labels: "WIP" - exempt-pr-labels: "WIP" - repo-token: "${{ secrets.GITHUB_TOKEN }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml deleted file mode 100644 index 2d20d64..0000000 --- a/.github/workflows/download_pipeline.yml +++ /dev/null @@ -1,86 +0,0 @@ -name: Test successful pipeline download with 'nf-core download' - -# Run the workflow when: -# - dispatched manually -# - when a PR is opened or reopened to master branch -# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. -on: - workflow_dispatch: - inputs: - testbranch: - description: "The specific branch you wish to utilize for the test execution of nf-core download." - required: true - default: "dev" - pull_request: - types: - - opened - - edited - - synchronize - branches: - - master - pull_request_target: - branches: - - master - -env: - NXF_ANSI_LOG: false - -jobs: - download: - runs-on: ubuntu-latest - steps: - - name: Install Nextflow - uses: nf-core/setup-nextflow@v2 - - - name: Disk space cleanup - uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 - - - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 - with: - python-version: "3.12" - architecture: "x64" - - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 - with: - singularity-version: 3.8.3 - - - name: Install dependencies - run: | - python -m pip install --upgrade pip - pip install git+https://github.com/nf-core/tools.git@dev - - - name: Get the repository name and current branch set as environment variable - run: | - echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} - echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} - echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} - - - name: Download the pipeline - env: - NXF_SINGULARITY_CACHEDIR: ./ - run: | - nf-core download ${{ env.REPO_LOWERCASE }} \ - --revision ${{ env.REPO_BRANCH }} \ - --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ - --compress "none" \ - --container-system 'singularity' \ - --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ - --container-cache-utilisation 'amend' \ - --download-configuration - - - name: Inspect download - run: tree ./${{ env.REPOTITLE_LOWERCASE }} - - - name: Run the downloaded pipeline (stub) - id: stub_run_pipeline - continue-on-error: true - env: - NXF_SINGULARITY_CACHEDIR: ./ - NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results - - name: Run the downloaded pipeline (stub run not supported) - id: run_pipeline - if: ${{ job.steps.stub_run_pipeline.status == failure() }} - env: - NXF_SINGULARITY_CACHEDIR: ./ - NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 6ce9fa3..60a415d 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -4,7 +4,7 @@ on: types: [created] jobs: - fix-linting: + deploy: # Only run if comment is on a PR with the main repo, and if it contains the magic keywords if: > contains(github.event.comment.html_url, '/pull/') && @@ -44,37 +44,12 @@ jobs: run: prettier --write ${GITHUB_WORKSPACE} - name: Commit & push changes - id: commit-and-push - if: steps.pre-commit.outcome == 'failure' + if: steps.prettier_status.outputs.result == 'fail' run: | git config user.email "105875386+sanger-tolsoft@users.noreply.github.com" git config user.name "sanger-tolsoft" git config push.default upstream git add . git status - git commit -m "[automated] Fix code linting" + git commit -m "[automated] Fix linting with Prettier" git push - - - name: react if linting errors were fixed - id: react-if-fixed - if: steps.commit-and-push.outcome == 'success' - uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 - with: - comment-id: ${{ github.event.comment.id }} - reactions: hooray - - - name: react if linting errors were not fixed - if: steps.commit-and-push.outcome == 'failure' - uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 - with: - comment-id: ${{ github.event.comment.id }} - reactions: confused - - - name: react if linting errors were not fixed - if: steps.commit-and-push.outcome == 'failure' - uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 - with: - issue-number: ${{ github.event.issue.number }} - body: | - @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually. - See [CI log](https://github.com/nf-core/readmapping/actions/runs/${{ github.run_id }}) for more details. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 1fcafe8..858d622 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,34 +11,74 @@ on: types: [published] jobs: - pre-commit: + EditorConfig: runs-on: ubuntu-latest steps: - - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + - uses: actions/checkout@v3 - - name: Set up Python 3.12 - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 + - uses: actions/setup-node@v3 + + - name: Install editorconfig-checker + run: npm install -g editorconfig-checker + + - name: Run ECLint check + run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') + + Prettier: + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v3 + + - uses: actions/setup-node@v3 + + - name: Install Prettier + run: npm install -g prettier + + - name: Run Prettier --check + run: prettier --check ${GITHUB_WORKSPACE} + + PythonBlack: + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v3 + + - name: Check code lints with Black + uses: psf/black@stable + + # If the above check failed, post a comment on the PR explaining the failure + - name: Post PR comment + if: failure() + uses: mshick/add-pr-comment@v1 with: - python-version: "3.12" + message: | + ## Python linting (`black`) is failing + + To keep the code consistent with lots of contributors, we run automated code consistency checks. + To fix this CI test, please run: + + * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` + * Fix formatting errors in your pipeline: `black .` + + Once you push these changes the test should pass, and you can hide this comment :+1: - - name: Install pre-commit - run: pip install pre-commit + We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! - - name: Run pre-commit - run: pre-commit run --all-files + Thanks again for your contribution! + repo-token: ${{ secrets.GITHUB_TOKEN }} + allow-repeats: false nf-core: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + uses: actions/checkout@v3 - name: Install Nextflow - uses: nf-core/setup-nextflow@v2 + uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 + - uses: actions/setup-python@v4 with: - python-version: "3.12" + python-version: "3.7" architecture: "x64" - name: Install dependencies @@ -59,7 +99,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4 + uses: actions/upload-artifact@v3 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 40acc23..3963518 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,17 +11,17 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3 + uses: dawidd6/action-download-artifact@v2 with: workflow: linting.yml workflow_conclusion: completed - name: Get PR number id: pr_number - run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT + run: echo "name=pr_number::$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 + uses: marocchino/sticky-pull-request-comment@v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml deleted file mode 100644 index 03ecfcf..0000000 --- a/.github/workflows/release-announcements.yml +++ /dev/null @@ -1,75 +0,0 @@ -name: release-announcements -# Automatic release toot and tweet anouncements -on: - release: - types: [published] - workflow_dispatch: - -jobs: - toot: - runs-on: ubuntu-latest - steps: - - name: get topics and convert to hashtags - id: get_topics - run: | - echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" >> $GITHUB_OUTPUT - - - uses: rzr/fediverse-action@master - with: - access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} - host: "mstdn.science" # custom host if not "mastodon.social" (default) - # GitHub event payload - # https://docs.github.com/en/developers/webhooks-and-events/webhooks/webhook-events-and-payloads#release - message: | - Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! - - Please see the changelog: ${{ github.event.release.html_url }} - - ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics - - send-tweet: - runs-on: ubuntu-latest - - steps: - - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 - with: - python-version: "3.10" - - name: Install dependencies - run: pip install tweepy==4.14.0 - - name: Send tweet - shell: python - run: | - import os - import tweepy - - client = tweepy.Client( - access_token=os.getenv("TWITTER_ACCESS_TOKEN"), - access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"), - consumer_key=os.getenv("TWITTER_CONSUMER_KEY"), - consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"), - ) - tweet = os.getenv("TWEET") - client.create_tweet(text=tweet) - env: - TWEET: | - Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! - - Please see the changelog: ${{ github.event.release.html_url }} - TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }} - TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }} - TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }} - TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }} - - bsky-post: - runs-on: ubuntu-latest - steps: - - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0 - with: - post: | - Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! - - Please see the changelog: ${{ github.event.release.html_url }} - env: - BSKY_IDENTIFIER: ${{ secrets.BSKY_IDENTIFIER }} - BSKY_PASSWORD: ${{ secrets.BSKY_PASSWORD }} - # From 94a10f08c2a651164378ef20f4a282c22dfd6287 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 12:57:58 +0100 Subject: [PATCH 065/111] schema update --- assets/email_template.html | 14 +++++------ assets/email_template.txt | 10 ++++---- assets/methods_description_template.yml | 8 +++--- assets/multiqc_config.yml | 10 ++++---- assets/nf-core-readmapping_logo_light.png | Bin 107268 -> 0 bytes assets/schema_input.json | 4 +-- conf/modules.config | 8 ------ modules.json | 10 ++++++++ nextflow.config | 2 +- nextflow_schema.json | 23 ++++++++++-------- .../utils_nfcore_readmapping_pipeline/main.nf | 1 - 11 files changed, 47 insertions(+), 43 deletions(-) delete mode 100644 assets/nf-core-readmapping_logo_light.png diff --git a/assets/email_template.html b/assets/email_template.html index 00e284a..76cef57 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -4,21 +4,21 @@ - - nf-core/readmapping Pipeline Report + + sanger-tol/readmapping Pipeline Report
    -

    nf-core/readmapping ${version}

    +

    sanger-tol/readmapping ${version}

    Run Name: $runName

    <% if (!success){ out << """
    -

    nf-core/readmapping execution completed unsuccessfully!

    +

    sanger-tol/readmapping execution completed unsuccessfully!

    The exit status of the task that caused the workflow execution to fail was: $exitStatus.

    The full error message was:

    ${errorReport}
    @@ -27,7 +27,7 @@

    nf-core/readmapping execution complete } else { out << """
    - nf-core/readmapping execution completed successfully! + sanger-tol/readmapping execution completed successfully!
    """ } @@ -44,8 +44,8 @@

    Pipeline Configuration:

    -

    nf-core/readmapping

    -

    https://github.com/nf-core/readmapping

    +

    sanger-tol/readmapping

    +

    https://github.com/sanger-tol/readmapping

    diff --git a/assets/email_template.txt b/assets/email_template.txt index 9e15bc6..71be3db 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -4,15 +4,15 @@ |\\ | |__ __ / ` / \\ |__) |__ } { | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, `._,._,' - nf-core/readmapping ${version} + sanger-tol/readmapping ${version} ---------------------------------------------------- Run Name: $runName <% if (success){ - out << "## nf-core/readmapping execution completed successfully! ##" + out << "## sanger-tol/readmapping execution completed successfully! ##" } else { out << """#################################################### -## nf-core/readmapping execution completed unsuccessfully! ## +## sanger-tol/readmapping execution completed unsuccessfully! ## #################################################### The exit status of the task that caused the workflow execution to fail was: $exitStatus. The full error message was: @@ -35,5 +35,5 @@ Pipeline Configuration: <% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> -- -nf-core/readmapping -https://github.com/nf-core/readmapping +sanger-tol/readmapping +https://github.com/sanger-tol/readmapping diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml index adf3c95..1b0e822 100644 --- a/assets/methods_description_template.yml +++ b/assets/methods_description_template.yml @@ -1,13 +1,13 @@ -id: "nf-core-readmapping-methods-description" +id: "sanger-tol-readmapping-methods-description" description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." -section_name: "nf-core/readmapping Methods Description" -section_href: "https://github.com/nf-core/readmapping" +section_name: "sanger-tol/readmapping Methods Description" +section_href: "https://github.com/sanger-tol/readmapping" plot_type: "html" ## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline ## You inject any metadata in the Nextflow '${workflow}' object data: |

    Methods

    -

    Data was processed using nf-core/readmapping v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

    +

    Data was processed using sanger-tol/readmapping v${workflow.manifest.version} ${doi_text} of the (Sanger-ToL collection of workflows), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

    The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

    ${workflow.commandLine}

    ${tool_citations}

    diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 4aa8ced..fd62d13 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,15 +1,15 @@ report_comment: > - - This report has been generated by the nf-core/readmapping + + This report has been generated by the sanger-tol/readmapping analysis pipeline. For information about how to interpret these results, please see the documentation. - + report_section_order: - "nf-core-readmapping-methods-description": + "sanger-tol-readmapping-methods-description": order: -1000 software_versions: order: -1001 - "nf-core-readmapping-summary": + "sanger-tol-readmapping-summary": order: -1002 export_plots: true diff --git a/assets/nf-core-readmapping_logo_light.png b/assets/nf-core-readmapping_logo_light.png deleted file mode 100644 index 2a6d405e34178e0ed71229fa9372710a6a82572c..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 107268 zcmeFYXH-+$)<4WS78C?Liijd08j#+l7X=YRm2Q9l3WN?y@2Dsox+Fjd2uM|WFQJM^ z2?Row7Meonp%WmKcZYNDJ&y7J{(gGamoUQKbItmjbImmq_Ebxij^-u}6%`d7_{k$( zDyj>IR8(i4TsRMW0~xo(Q&IilXQ!Cr7%+Ar8ipu+=N9+_|unu#(qQsr? zJEk=+OT~*Mx(9zgMv2>l#jI;aCy#W;>l@ZUXai@XxSEaSw@t52=8SpnaC&v#e-&(o zt1GOs!YxYU=KXO#sXadQ&+b4tKTG;@AcbsaEWS#!LtjcXS+u{Uqh=LRF=~gwlm;tt 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"http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/readmapping/master/assets/schema_input.json", - "title": "nf-core/readmapping pipeline - params.input schema", + "$id": "https://raw.githubusercontent.com/sanger-tol/readmapping/master/assets/schema_input.json", + "title": "sanger-tol/readmapping pipeline - params.input schema", "description": "Schema for the file provided with params.input", "type": "array", "items": { diff --git a/conf/modules.config b/conf/modules.config index 1bb619f..089592e 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -85,14 +85,6 @@ process { ext.args = { (meta.datatype == "pacbio" ? "-y pbccs " : "") + "-O bam" } } - withName: SAMPLESHEET_CHECK { - publishDir = [ - path: { "${params.outdir}/pipeline_info/readmapping" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: '.*:CONVERT_STATS:SAMTOOLS_.*' { publishDir = [ path: { "${params.outdir}/read_mapping/${meta.datatype}" }, diff --git a/modules.json b/modules.json index 8c2a69a..113030b 100644 --- a/modules.json +++ b/modules.json @@ -30,6 +30,11 @@ "git_sha": "82024cf6325d2ee194e7f056d841ecad2f6856e9", "installed_by": ["modules"] }, + "fastqc": { + "branch": "master", + "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", + "installed_by": ["modules"] + }, "gunzip": { "branch": "master", "git_sha": "4e5f4687318f24ba944a13609d3ea6ebd890737d", @@ -40,6 +45,11 @@ "git_sha": "e83b347b3e674de6bb1bb7bdf9b2674768c8e0fe", "installed_by": ["modules"] }, + "multiqc": { + "branch": "master", + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", + "installed_by": ["modules"] + }, "samtools/faidx": { "branch": "master", "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", diff --git a/nextflow.config b/nextflow.config index ad85efc..585cb46 100644 --- a/nextflow.config +++ b/nextflow.config @@ -21,7 +21,7 @@ params { // Boilerplate options - outdir = "./results" + outdir = null publish_dir_mode = 'copy' email = null email_on_fail = null diff --git a/nextflow_schema.json b/nextflow_schema.json index ab8f129..bc6ef19 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -33,7 +33,7 @@ "type": "string", "description": "Path to directory or tar.gz archive for pre-built PacBio vector database.", "format": "path", - "default": "assets/vectorDB.tar.gz", + "default": "/Users/tc25/projects/readmapping/assets/vectorDB.tar.gz", "fa_icon": "fas fa-bezier-curve" }, "email": { @@ -233,6 +233,10 @@ "fa_icon": "fas fa-palette", "hidden": true }, + "monochromeLogs": { + "type": "boolean", + "hidden": true + }, "hook_url": { "type": "string", "description": "Incoming hook URL for messaging service", @@ -250,14 +254,14 @@ "multiqc_logo": { "type": "string", "description": "Directory to keep pipeline Nextflow logs and reports.", - "default": "./results/pipeline_info/readmapping", "fa_icon": "fas fa-cogs", "hidden": true }, "multiqc_methods_description": { "type": "string", "description": "Custom MultiQC yaml file containing HTML including a methods description.", - "fa_icon": "fas fa-cog" + "fa_icon": "fas fa-cog", + "hidden": true }, "validate_params": { "type": "boolean", @@ -286,6 +290,11 @@ "description": "Validation of parameters in lenient more.", "hidden": true, "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." + }, + "schema_ignore_params": { + "type": "string", + "default": "genomes", + "hidden": true } } } @@ -309,11 +318,5 @@ { "$ref": "#/definitions/generic_options" } - ], - "properties": { - "schema_ignore_params": { - "type": "string", - "default": "genomes" - } - } + ] } diff --git a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf index aacdb31..5320cb5 100644 --- a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf @@ -171,7 +171,6 @@ def validateInputParameters() { if (!params.fasta) { log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - System.exit(1) } } From 26b3818050e84f6f1161be7c2dfb574f75dab1e9 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 12:59:55 +0100 Subject: [PATCH 066/111] update .gitignore --- .gitignore | 1 + 1 file changed, 1 insertion(+) diff --git a/.gitignore b/.gitignore index 5124c9a..047c82c 100644 --- a/.gitignore +++ b/.gitignore @@ -2,6 +2,7 @@ work/ data/ results/ +null/ .DS_Store testing/ testing* From ed898a630e37db9c06d08a49e1aae5f2613e1d1f Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 13:40:58 +0100 Subject: [PATCH 067/111] clean up --- .github/ISSUE_TEMPLATE/config.yml | 7 + .github/workflows/clean-up.yml | 24 + .../execution_report_2024-07-19_13-12-27.html | 1075 ----------------- ...xecution_timeline_2024-07-19_13-12-27.html | 227 ---- .../execution_trace_2024-07-19_13-08-38.txt | 1 - .../execution_trace_2024-07-19_13-09-15.txt | 1 - .../execution_trace_2024-07-19_13-10-06.txt | 1 - .../execution_trace_2024-07-19_13-11-56.txt | 1 - .../execution_trace_2024-07-19_13-12-27.txt | 6 - .../pipeline_dag_2024-07-19_13-12-27.html | 423 ------- subworkflows/local/prepare_genome.nf | 8 +- 11 files changed, 35 insertions(+), 1739 deletions(-) create mode 100644 .github/ISSUE_TEMPLATE/config.yml create mode 100644 .github/workflows/clean-up.yml delete mode 100644 null/pipeline_info/execution_report_2024-07-19_13-12-27.html delete mode 100644 null/pipeline_info/execution_timeline_2024-07-19_13-12-27.html delete mode 100644 null/pipeline_info/execution_trace_2024-07-19_13-08-38.txt delete mode 100644 null/pipeline_info/execution_trace_2024-07-19_13-09-15.txt delete mode 100644 null/pipeline_info/execution_trace_2024-07-19_13-10-06.txt delete mode 100644 null/pipeline_info/execution_trace_2024-07-19_13-11-56.txt delete mode 100644 null/pipeline_info/execution_trace_2024-07-19_13-12-27.txt delete mode 100644 null/pipeline_info/pipeline_dag_2024-07-19_13-12-27.html diff --git a/.github/ISSUE_TEMPLATE/config.yml b/.github/ISSUE_TEMPLATE/config.yml new file mode 100644 index 0000000..68bef12 --- /dev/null +++ b/.github/ISSUE_TEMPLATE/config.yml @@ -0,0 +1,7 @@ +contact_links: + - name: Join nf-core + url: https://nf-co.re/join + about: Please join the nf-core community here + - name: "Slack #readmapping channel" + url: https://nfcore.slack.com/channels/readmapping + about: Discussion about the nf-core/readmapping pipeline diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml new file mode 100644 index 0000000..0b6b1f2 --- /dev/null +++ b/.github/workflows/clean-up.yml @@ -0,0 +1,24 @@ +name: "Close user-tagged issues and PRs" +on: + schedule: + - cron: "0 0 * * 0" # Once a week + +jobs: + clean-up: + runs-on: ubuntu-latest + permissions: + issues: write + pull-requests: write + steps: + - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 + with: + stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." + stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." + close-issue-message: "This issue was closed because it has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor and then staled for 20 days with no activity." + days-before-stale: 30 + days-before-close: 20 + days-before-pr-close: -1 + any-of-labels: "awaiting-changes,awaiting-feedback" + exempt-issue-labels: "WIP" + exempt-pr-labels: "WIP" + repo-token: "${{ secrets.GITHUB_TOKEN }}" diff --git a/null/pipeline_info/execution_report_2024-07-19_13-12-27.html b/null/pipeline_info/execution_report_2024-07-19_13-12-27.html deleted file mode 100644 index eb661c4..0000000 --- a/null/pipeline_info/execution_report_2024-07-19_13-12-27.html +++ /dev/null @@ -1,1075 +0,0 @@ - - - - - - - - - - - [fervent_volta] Nextflow Workflow Report - - - - - - - -
    -
    - -

    Nextflow workflow report

    -

    [fervent_volta]

    - - -
    -

    Workflow execution completed unsuccessfully!

    -

    The exit status of the task that caused the workflow execution to fail was: 125.

    -

    The full error message was:

    -
    Error executing process > 'SANGERTOL_READMAPPING:READMAPPING:INPUT_CHECK:SAMTOOLS_FLAGSTAT (31231_4%231.subset)'
    -
    -Caused by:
    -  Process `SANGERTOL_READMAPPING:READMAPPING:INPUT_CHECK:SAMTOOLS_FLAGSTAT (31231_4%231.subset)` terminated with an error exit status (125)
    -
    -
    -Command executed:
    -
    -  samtools \
    -      flagstat \
    -      --threads 1 \
    -      31231_4%231.subset.cram \
    -      > 31231_4%231.subset.flagstat
    -  
    -  cat <<-END_VERSIONS > versions.yml
    -  "SANGERTOL_READMAPPING:READMAPPING:INPUT_CHECK:SAMTOOLS_FLAGSTAT":
    -      samtools: $(echo $(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*$//')
    -  END_VERSIONS
    -
    -Command exit status:
    -  125
    -
    -Command output:
    -  (empty)
    -
    -Command error:
    -  docker: Cannot connect to the Docker daemon at unix:///Users/tc25/.docker/run/docker.sock. Is the docker daemon running?.
    -  See 'docker run --help'.
    -
    -Work dir:
    -  /Users/tc25/projects/readmapping/work/e8/9067b8023fae169fcaab7f14d70532
    -
    -Tip: you can try to figure out what's wrong by changing to the process work dir and showing the script file named `.command.sh`
    -
    - - -
    -
    Run times
    -
    - 19-Jul-2024 13:12:27 - 19-Jul-2024 13:12:31 - (duration: 3.9s) -
    - -
    -
    -
      0 succeeded  
    -
      0 cached  
    -
      0 ignored  
    -
      5 failed  
    -
    -
    - -
    Nextflow command
    -
    nextflow run main.nf -profile test,docker
    -
    - -
    -
    CPU-Hours
    -
    (a few seconds)
    - -
    Launch directory
    -
    /Users/tc25/projects/readmapping
    - -
    Work directory
    -
    /Users/tc25/projects/readmapping/work
    - -
    Project directory
    -
    /Users/tc25/projects/readmapping
    - - -
    Script name
    -
    main.nf
    - - - -
    Script ID
    -
    45f0636c991004b352e3aaf250ab151a
    - - -
    Workflow session
    -
    4bbfc38a-1717-4167-a76e-d814cffdf881
    - - - -
    Workflow profile
    -
    test,docker
    - - - - - -
    Nextflow version
    -
    version 24.04.2, build 5914 (29-05-2024 06:19 UTC)
    -
    -
    -
    - -
    -

    Resource Usage

    -

    These plots give an overview of the distribution of resource usage for each process.

    - -

    CPU

    - -
    -
    -
    -
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    -
    -
    - -
    - -

    Memory

    - -
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    -
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    -
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    - -

    Job Duration

    - -
    -
    -
    -
    -
    -
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    - -

    I/O

    - -
    -
    -
    -
    -
    -
    -
    -
    -
    - -
    -
    -

    Tasks

    -

    This table shows information about each task in the workflow. Use the search box on the right - to filter rows for specific values. Clicking headers will sort the table by that value and - scrolling side to side will reveal more columns.

    -
    - - -
    -
    -
    -
    -
    - -
    - (tasks table omitted because the dataset is too big) -
    -
    - -
    -
    - Generated by Nextflow, version 24.04.2 -
    -
    - - - - - diff --git a/null/pipeline_info/execution_timeline_2024-07-19_13-12-27.html b/null/pipeline_info/execution_timeline_2024-07-19_13-12-27.html deleted file mode 100644 index d822887..0000000 --- a/null/pipeline_info/execution_timeline_2024-07-19_13-12-27.html +++ /dev/null @@ -1,227 +0,0 @@ - - - - - - - - - - - - - -
    -

    Processes execution timeline

    -

    - Launch time:
    - Elapsed time:
    - Legend: job wall time / memory usage (RAM) -

    -
    -
    - - - - - - - diff --git a/null/pipeline_info/execution_trace_2024-07-19_13-08-38.txt b/null/pipeline_info/execution_trace_2024-07-19_13-08-38.txt deleted file mode 100644 index 6b739ac..0000000 --- a/null/pipeline_info/execution_trace_2024-07-19_13-08-38.txt +++ /dev/null @@ -1 +0,0 @@ -task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar diff --git a/null/pipeline_info/execution_trace_2024-07-19_13-09-15.txt b/null/pipeline_info/execution_trace_2024-07-19_13-09-15.txt deleted file mode 100644 index 6b739ac..0000000 --- a/null/pipeline_info/execution_trace_2024-07-19_13-09-15.txt +++ /dev/null @@ -1 +0,0 @@ -task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar diff --git a/null/pipeline_info/execution_trace_2024-07-19_13-10-06.txt b/null/pipeline_info/execution_trace_2024-07-19_13-10-06.txt deleted file mode 100644 index 6b739ac..0000000 --- a/null/pipeline_info/execution_trace_2024-07-19_13-10-06.txt +++ /dev/null @@ -1 +0,0 @@ -task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar diff --git a/null/pipeline_info/execution_trace_2024-07-19_13-11-56.txt b/null/pipeline_info/execution_trace_2024-07-19_13-11-56.txt deleted file mode 100644 index 6b739ac..0000000 --- a/null/pipeline_info/execution_trace_2024-07-19_13-11-56.txt +++ /dev/null @@ -1 +0,0 @@ -task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar diff --git a/null/pipeline_info/execution_trace_2024-07-19_13-12-27.txt b/null/pipeline_info/execution_trace_2024-07-19_13-12-27.txt deleted file mode 100644 index 4036a10..0000000 --- a/null/pipeline_info/execution_trace_2024-07-19_13-12-27.txt +++ /dev/null @@ -1,6 +0,0 @@ -task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar -3 84/8e6b8e 9043 SANGERTOL_READMAPPING:READMAPPING:INPUT_CHECK:SAMTOOLS_FLAGSTAT (31231_3%231.subset) FAILED 125 2024-07-19 13:12:30.320 658ms 638ms - - - - - -4 e8/9067b8 9058 SANGERTOL_READMAPPING:READMAPPING:INPUT_CHECK:SAMTOOLS_FLAGSTAT (31231_4%231.subset) FAILED 125 2024-07-19 13:12:30.434 549ms 431ms - - - - - -1 44/44b4e8 9009 SANGERTOL_READMAPPING:READMAPPING:UNTAR (vectorDB.tar.gz) FAILED 125 2024-07-19 13:12:29.992 1s 986ms - - - - - -2 5d/b824a4 9016 SANGERTOL_READMAPPING:READMAPPING:PREPARE_GENOME:GUNZIP (GCA_922984935.2.subset.fasta.gz) FAILED 125 2024-07-19 13:12:30.113 908ms 886ms - - - - - -5 dc/f440ee 9089 SANGERTOL_READMAPPING:READMAPPING:INPUT_CHECK:SAMTOOLS_FLAGSTAT (35528_2%231.subset) FAILED 125 2024-07-19 13:12:30.689 359ms 285ms - - - - - diff --git a/null/pipeline_info/pipeline_dag_2024-07-19_13-12-27.html b/null/pipeline_info/pipeline_dag_2024-07-19_13-12-27.html deleted file mode 100644 index f34e79b..0000000 --- a/null/pipeline_info/pipeline_dag_2024-07-19_13-12-27.html +++ /dev/null @@ -1,423 +0,0 @@ - - - - - - -
    -flowchart TB
    -    subgraph " "
    -    v2["Channel.fromPath"]
    -    v3["Channel.fromPath"]
    -    v4["channel.fromSamplesheet"]
    -    v31[" "]
    -    v35["interleave"]
    -    v42["sort_bam"]
    -    v54[" "]
    -    v55[" "]
    -    v69["interleave"]
    -    v76["sort_bam"]
    -    v88[" "]
    -    v89[" "]
    -    v105[" "]
    -    v106["qname"]
    -    v133["bam_format"]
    -    v134["bam_index_extension"]
    -    v135["cigar_paf_format"]
    -    v136["cigar_bam"]
    -    v146[" "]
    -    v147[" "]
    -    v159[" "]
    -    v160["qname"]
    -    v187["bam_format"]
    -    v188["bam_index_extension"]
    -    v189["cigar_paf_format"]
    -    v190["cigar_bam"]
    -    v200[" "]
    -    v201[" "]
    -    v211["bam_format"]
    -    v212["bam_index_extension"]
    -    v213["cigar_paf_format"]
    -    v214["cigar_bam"]
    -    v224[" "]
    -    v225[" "]
    -    v248["keepbed"]
    -    v249["bedout"]
    -    v257["qname"]
    -    end
    -    subgraph SANGERTOL_READMAPPING
    -    subgraph READMAPPING
    -    subgraph INPUT_CHECK
    -    v12([SAMTOOLS_FLAGSTAT])
    -    v5(( ))
    -    end
    -    subgraph PREPARE_GENOME
    -    v21([GUNZIP])
    -    v24([UNMASK])
    -    v26([BWAMEM2_INDEX])
    -    v19(( ))
    -    v23(( ))
    -    v28(( ))
    -    v29(( ))
    -    end
    -    v32([UNTAR])
    -    subgraph ALIGN_HIC
    -    v36([SAMTOOLS_FASTQ])
    -    v43([BWAMEM2_MEM])
    -    v56([SAMTOOLS_MERGE])
    -    v63([SAMTOOLS_SORMADUP])
    -    v50(( ))
    -    end
    -    subgraph ALIGN_ILLUMINA
    -    v70([SAMTOOLS_FASTQ])
    -    v77([BWAMEM2_MEM])
    -    v90([SAMTOOLS_MERGE])
    -    v97([SAMTOOLS_SORMADUP])
    -    v84(( ))
    -    end
    -    subgraph ALIGN_HIFI
    -    subgraph FILTER_PACBIO
    -    v107([SAMTOOLS_CONVERT])
    -    v116([SAMTOOLS_COLLATETOFASTA])
    -    v119([BLAST_BLASTN])
    -    v122([PACBIO_FILTER])
    -    v129([SAMTOOLS_FILTERTOFASTQ])
    -    v125(( ))
    -    end
    -    v137([MINIMAP2_ALIGN])
    -    v148([SAMTOOLS_MERGE])
    -    end
    -    subgraph ALIGN_CLR
    -    subgraph FILTER_PACBIO
    -    v161([SAMTOOLS_CONVERT])
    -    v170([SAMTOOLS_COLLATETOFASTA])
    -    v173([BLAST_BLASTN])
    -    v176([PACBIO_FILTER])
    -    v183([SAMTOOLS_FILTERTOFASTQ])
    -    v179(( ))
    -    end
    -    v191([MINIMAP2_ALIGN])
    -    v202([SAMTOOLS_MERGE])
    -    end
    -    subgraph ALIGN_ONT
    -    v215([MINIMAP2_ALIGN])
    -    v226([SAMTOOLS_MERGE])
    -    end
    -    v243([SAMTOOLS_REHEADER])
    -    subgraph CONVERT_STATS
    -    v250([CRUMBLE])
    -    v258([SAMTOOLS_VIEW])
    -    v268([SAMTOOLS_STATS])
    -    v272([SAMTOOLS_FLAGSTAT])
    -    v276([SAMTOOLS_IDXSTATS])
    -    v247(( ))
    -    v267(( ))
    -    end
    -    v283([CUSTOM_DUMPSOFTWAREVERSIONS])
    -    v13(( ))
    -    v142(( ))
    -    v242(( ))
    -    end
    -    end
    -    subgraph " "
    -    v37[" "]
    -    v38[" "]
    -    v39[" "]
    -    v44[" "]
    -    v45[" "]
    -    v46[" "]
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    -    v57[" "]
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    -    v78[" "]
    -    v79[" "]
    -    v80[" "]
    -    v81[" "]
    -    v91[" "]
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    -    v98[" "]
    -    v99[" "]
    -    v108[" "]
    -    v109[" "]
    -    v110[" "]
    -    v111[" "]
    -    v112[" "]
    -    v113[" "]
    -    v138[" "]
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    -    v259[" "]
    -    v260[" "]
    -    v261[" "]
    -    v262[" "]
    -    v263[" "]
    -    v264[" "]
    -    v269["stats"]
    -    v273["flagstat"]
    -    v277["idxstats"]
    -    v284[" "]
    -    v285[" "]
    -    v286[" "]
    -    end
    -    v2 --> v19
    -    v3 --> v242
    -    v4 --> v5
    -    v5 --> v12
    -    v12 --> v5
    -    v12 --> v13
    -    v19 --> v21
    -    v21 --> v13
    -    v21 --> v23
    -    v23 --> v24
    -    v24 --> v26
    -    v24 --> v13
    -    v24 --> v28
    -    v26 --> v13
    -    v26 --> v29
    -    v31 --> v32
    -    v32 --> v119
    -    v32 --> v173
    -    v32 --> v13
    -    v35 --> v36
    -    v5 --> v36
    -    v36 --> v43
    -    v36 --> v39
    -    v36 --> v38
    -    v36 --> v37
    -    v36 --> v13
    -    v42 --> v43
    -    v28 --> v43
    -    v29 --> v43
    -    v43 --> v47
    -    v43 --> v46
    -    v43 --> v45
    -    v43 --> v44
    -    v43 --> v13
    -    v43 --> v50
    -    v54 --> v56
    -    v55 --> v56
    -    v50 --> v56
    -    v56 --> v59
    -    v56 --> v58
    -    v56 --> v57
    -    v56 --> v13
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    - - - diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 5264569..c595f9b 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -8,7 +8,7 @@ include { UNTAR } from '../../modules/nf-core/untar/main' include { BWAMEM2_INDEX } from '../../modules/nf-core/bwamem2/index/main' -workflow PREPARE_GENOME { +workflow PREPARE_GENOME { take: fasta // channel: [ meta, /path/to/fasta ] @@ -31,7 +31,7 @@ workflow PREPARE_GENOME { // Unmask genome fasta UNMASK ( ch_fasta ) - ch_versions = ch_versions.mix ( UNMASK.out.versions ) + ch_versions = ch_versions.mix ( UNMASK.out.versions.first() ) // Generate BWA index @@ -43,14 +43,14 @@ workflow PREPARE_GENOME { if ( params.bwamem2_index.endsWith('.tar.gz') ) { ch_bwamem2_index = UNTAR ( ch_bwamem ).untar - ch_versions = ch_versions.mix ( UNTAR.out.versions ) + ch_versions = ch_versions.mix ( UNTAR.out.versions.first() ) } else { ch_bwamem2_index = ch_bwamem } } else { ch_bwamem2_index = BWAMEM2_INDEX ( UNMASK.out.fasta ).index - ch_versions = ch_versions.mix ( BWAMEM2_INDEX.out.versions ) + ch_versions = ch_versions.mix ( BWAMEM2_INDEX.out.versions.first() ) } From 20d2a51977a0df124dc5f96582cb679e423b4dff Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 13:41:22 +0100 Subject: [PATCH 068/111] clean up --- .github/ISSUE_TEMPLATE/config.yml | 7 ------- .github/workflows/clean-up.yml | 24 ------------------------ 2 files changed, 31 deletions(-) delete mode 100644 .github/ISSUE_TEMPLATE/config.yml delete mode 100644 .github/workflows/clean-up.yml diff --git a/.github/ISSUE_TEMPLATE/config.yml b/.github/ISSUE_TEMPLATE/config.yml deleted file mode 100644 index 68bef12..0000000 --- a/.github/ISSUE_TEMPLATE/config.yml +++ /dev/null @@ -1,7 +0,0 @@ -contact_links: - - name: Join nf-core - url: https://nf-co.re/join - about: Please join the nf-core community here - - name: "Slack #readmapping channel" - url: https://nfcore.slack.com/channels/readmapping - about: Discussion about the nf-core/readmapping pipeline diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml deleted file mode 100644 index 0b6b1f2..0000000 --- a/.github/workflows/clean-up.yml +++ /dev/null @@ -1,24 +0,0 @@ -name: "Close user-tagged issues and PRs" -on: - schedule: - - cron: "0 0 * * 0" # Once a week - -jobs: - clean-up: - runs-on: ubuntu-latest - permissions: - issues: write - pull-requests: write - steps: - - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 - with: - stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." - stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." - close-issue-message: "This issue was closed because it has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor and then staled for 20 days with no activity." - days-before-stale: 30 - days-before-close: 20 - days-before-pr-close: -1 - any-of-labels: "awaiting-changes,awaiting-feedback" - exempt-issue-labels: "WIP" - exempt-pr-labels: "WIP" - repo-token: "${{ secrets.GITHUB_TOKEN }}" From af0d8a3221086ed3950e5c94343027811d985029 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 13:48:09 +0100 Subject: [PATCH 069/111] nf-core linting --- assets/methods_description_template.yml | 2 +- nextflow.config | 2 +- .../local/utils_nfcore_readmapping_pipeline/main.nf | 6 +----- 3 files changed, 3 insertions(+), 7 deletions(-) diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml index 1b0e822..edf4544 100644 --- a/assets/methods_description_template.yml +++ b/assets/methods_description_template.yml @@ -3,7 +3,7 @@ description: "Suggested text and references to use when describing pipeline usag section_name: "sanger-tol/readmapping Methods Description" section_href: "https://github.com/sanger-tol/readmapping" plot_type: "html" -## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline +## nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline ## You inject any metadata in the Nextflow '${workflow}' object data: |

    Methods

    diff --git a/nextflow.config b/nextflow.config index 585cb46..621d0d8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -233,7 +233,7 @@ manifest { description = 'Pipeline to map reads generated using different sequencing technologies against a genome assembly.' mainScript = 'main.nf' nextflowVersion = '!>=22.10.1' - version = '1.3.0' + version = 'dev: 1.3.0' doi = '10.5281/zenodo.6563577' } diff --git a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf index 5320cb5..cbae3b8 100644 --- a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf @@ -1,5 +1,5 @@ // -// Subworkflow with functionality specific to the {{ name }} pipeline +// Subworkflow with functionality specific to the sanger-tol/readmapping pipeline // /* @@ -271,10 +271,6 @@ def methodsDescriptionText(mqc_methods_yaml) { meta["nodoi_text"] = meta.manifest_map.doi ? "" : "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
  • " // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") meta["tool_bibliography"] = toolBibliographyText() From 14dc59143da08d500e8a13c0f0f184a89861d15d Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 13:50:34 +0100 Subject: [PATCH 070/111] revert some template changes --- conf/base.config | 2 +- conf/igenomes.config | 440 ------------------ conf/test.config | 2 +- conf/test_full.config | 2 +- docs/README.md | 6 +- docs/images/nf-core-readmapping_logo_dark.png | Bin 27679 -> 0 bytes .../images/nf-core-readmapping_logo_light.png | Bin 23332 -> 0 bytes docs/output.md | 5 +- docs/usage.md | 28 +- 9 files changed, 14 insertions(+), 471 deletions(-) delete mode 100644 conf/igenomes.config delete mode 100644 docs/images/nf-core-readmapping_logo_dark.png delete mode 100644 docs/images/nf-core-readmapping_logo_light.png diff --git a/conf/base.config b/conf/base.config index d2fe239..ca753f3 100644 --- a/conf/base.config +++ b/conf/base.config @@ -1,6 +1,6 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - nf-core/readmapping Nextflow base config file + sanger-tol/readmapping Nextflow base config file ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ diff --git a/conf/igenomes.config b/conf/igenomes.config deleted file mode 100644 index 3f11437..0000000 --- a/conf/igenomes.config +++ /dev/null @@ -1,440 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Nextflow config file for iGenomes paths -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Defines reference genomes using iGenome paths. - Can be used by any config that customises the base path using: - $params.igenomes_base / --igenomes_base ----------------------------------------------------------------------------------------- -*/ - -params { - // illumina iGenomes reference file paths - genomes { - 'GRCh37' { - fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" - } - 'GRCh38' { - fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'CHM13' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAIndex/" - bwamem2 = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAmem2Index/" - gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/CHM13/Annotation/Genes/genes.gtf" - gff = "ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/009/914/755/GCF_009914755.1_T2T-CHM13v2.0/GCF_009914755.1_T2T-CHM13v2.0_genomic.gff.gz" - mito_name = "chrM" - } - 'GRCm38' { - fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" - } - 'TAIR10' { - fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" - mito_name = "Mt" - } - 'EB2' { - fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" - } - 'UMD3.1' { - fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" - mito_name = "MT" - } - 'WBcel235' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" - mito_name = "MtDNA" - macs_gsize = "9e7" - } - 'CanFam3.1' { - fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" - mito_name = "MT" - } - 'GRCz10' { - fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'BDGP6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" - mito_name = "M" - macs_gsize = "1.2e8" - } - 'EquCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" - mito_name = "MT" - } - 'EB1' { - fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" - } - 'Galgal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Gm01' { - fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" - } - 'Mmul_1' { - fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" - mito_name = "MT" - } - 'IRGSP-1.0' { - fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'CHIMP2.1.4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" - mito_name = "MT" - } - 'Rnor_5.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Rnor_6.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'R64-1-1' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" - mito_name = "MT" - macs_gsize = "1.2e7" - } - 'EF2' { - fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.21e7" - } - 'Sbi1' { - fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" - } - 'Sscrofa10.2' { - fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" - mito_name = "MT" - } - 'AGPv3' { - fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'hg38' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'hg19' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" - } - 'mm10' { - fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" - } - 'bosTau8' { - fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'ce10' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "9e7" - } - 'canFam3' { - fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" - mito_name = "chrM" - } - 'danRer10' { - fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.37e9" - } - 'dm6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.2e8" - } - 'equCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" - mito_name = "chrM" - } - 'galGal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" - mito_name = "chrM" - } - 'panTro4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" - mito_name = "chrM" - } - 'rn6' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'sacCer3' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" - readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.2e7" - } - 'susScr3' { - fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" - mito_name = "chrM" - } - } -} diff --git a/conf/test.config b/conf/test.config index eec4b8f..e269f68 100644 --- a/conf/test.config +++ b/conf/test.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/readmapping -profile test, --outdir + nextflow run sanger-tol/readmapping -profile test, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/test_full.config b/conf/test_full.config index d9ecc0d..562b16d 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a full size pipeline test. Use as follows: - nextflow run nf-core/readmapping -profile test_full, --outdir + nextflow run sanger-tol/readmapping -profile test_full, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/docs/README.md b/docs/README.md index c22fb7f..87f5ca0 100644 --- a/docs/README.md +++ b/docs/README.md @@ -1,10 +1,8 @@ -# nf-core/readmapping: Documentation +# sanger-tol/readmapping: Documentation -The nf-core/readmapping documentation is split into the following pages: +The sanger-tol/readmapping documentation is split into the following pages: - [Usage](usage.md) - An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. - [Output](output.md) - An overview of the different results produced by the pipeline and how to interpret them. - -You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: 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z{gae?=iVY}=AhkwXP38vwzxr$yqLJz6|y%RByjyt4=>gM^f|e?U&3Gy4e4@Vua8Vu zJmWa}J_qc(X`R|^kSy~(rf;f2-u6|^*r0RQknte(afc_FE#hKwjrWs3&E+df1ziiN zo^B`kB$W51r0h8&Nf<;oof7-{-Tyj9BWje=YCvViGCd*-BD_l|NfVqfAYm85SN+}p zRsuI0yLZRw@_%C3u50+Zuz?f5T?~&yUIw`XQs<*Y<1+q6!JT{Tf0U LSRiXIyWaah2~7cx diff --git a/docs/output.md b/docs/output.md index cb7a9cf..e9b8b8c 100644 --- a/docs/output.md +++ b/docs/output.md @@ -1,8 +1,8 @@ -# nf-core/readmapping: Output +# sanger-tol/readmapping: Output ## Introduction -This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. +This document describes the output produced by the pipeline. The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. @@ -121,7 +121,6 @@ A number of genome-specific files are generated by the pipeline because they are - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline. - Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. - - Parameters used by the pipeline run: `params.json`. diff --git a/docs/usage.md b/docs/usage.md index 0e1cffc..f539695 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -73,38 +73,28 @@ You can also optionally supply a template SAM header using the `--header` option When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: ```bash -nextflow pull nf-core/readmapping +nextflow pull sanger-tol/readmapping ``` ### Reproducibility It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. -First, go to the [nf-core/readmapping releases page](https://github.com/nf-core/readmapping/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. +First, go to the [sanger-tol/readmapping releases page](https://github.com/sanger-tol/readmapping/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. -This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports. - -To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter. - -:::tip -If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles. -::: +This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. ## Core Nextflow arguments -:::note -These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). -::: +> **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). ### `-profile` Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. -Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below. +Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. -:::info -We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. -::: +> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). @@ -126,12 +116,8 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof - A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/) - `charliecloud` - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) -- `apptainer` - - A generic configuration profile to be used with [Apptainer](https://apptainer.org/) -- `wave` - - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. Use together with one of the above (requires Nextflow ` 24.03.0-edge` or later). - `conda` - - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer. + - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud. ### `-resume` From 64046740ec1b0edb389f33f14fdc9ea249689144 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 13:51:40 +0100 Subject: [PATCH 071/111] revert some template changes --- assets/sendmail_template.txt | 29 ++--------------------------- 1 file changed, 2 insertions(+), 27 deletions(-) diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt index 485d7d0..16c30af 100644 --- a/assets/sendmail_template.txt +++ b/assets/sendmail_template.txt @@ -9,10 +9,10 @@ Content-Type: text/html; charset=utf-8 $email_html --nfcoremimeboundary -Content-Type: image/png;name="nf-core-readmapping_logo.png" +Content-Type: image/png;name="sanger-tol-readmapping_logo.png" Content-Transfer-Encoding: base64 Content-ID: -Content-Disposition: inline; filename="nf-core-readmapping_logo_light.png" +Content-Disposition: inline; filename="sanger-tol-readmapping_logo.png" <% out << new File("$projectDir/docs/images/sanger-tol-readmapping_logo.png"). bytes. @@ -25,29 +25,4 @@ Content-Disposition: inline; filename="nf-core-readmapping_logo_light.png" flatten(). join( '\n' ) %> -<% -if (mqcFile){ -def mqcFileObj = new File("$mqcFile") -if (mqcFileObj.length() < mqcMaxSize){ -out << """ ---nfcoremimeboundary -Content-Type: text/html; name=\"multiqc_report\" -Content-Transfer-Encoding: base64 -Content-ID: -Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" - -${mqcFileObj. - bytes. - encodeBase64(). - toString(). - tokenize( '\n' )*. - toList()*. - collate( 76 )*. - collect { it.join() }. - flatten(). - join( '\n' )} -""" -}} -%> - --nfcoremimeboundary-- From 5224fdd58f50b8f40847918318cbb4599079ff41 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 13:52:37 +0100 Subject: [PATCH 072/111] revert some template changes --- assets/adaptivecard.json | 2 +- assets/email_template.html | 4 ++-- assets/email_template.txt | 8 -------- 3 files changed, 3 insertions(+), 11 deletions(-) diff --git a/assets/adaptivecard.json b/assets/adaptivecard.json index 73e7fa1..4cf56a6 100644 --- a/assets/adaptivecard.json +++ b/assets/adaptivecard.json @@ -17,7 +17,7 @@ "size": "Large", "weight": "Bolder", "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", - "text": "nf-core/readmapping v${version} - ${runName}", + "text": "sanger-tol/readmapping v${version} - ${runName}", "wrap": true }, { diff --git a/assets/email_template.html b/assets/email_template.html index 76cef57..5c0fae1 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -4,7 +4,7 @@ - + sanger-tol/readmapping Pipeline Report @@ -12,7 +12,7 @@ -

    sanger-tol/readmapping ${version}

    +

    sanger-tol/readmapping v${version}

    Run Name: $runName

    <% if (!success){ diff --git a/assets/email_template.txt b/assets/email_template.txt index 71be3db..89f6f33 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -1,11 +1,3 @@ ----------------------------------------------------- - ,--./,-. - ___ __ __ __ ___ /,-._.--~\\ - |\\ | |__ __ / ` / \\ |__) |__ } { - | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, - `._,._,' - sanger-tol/readmapping ${version} ----------------------------------------------------- Run Name: $runName <% if (success){ From 802aac2df26df7cb423ffd5212803f351a1d5f39 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 14:14:11 +0100 Subject: [PATCH 073/111] cleanup --- .github/workflows/ci.yml | 2 +- .github/workflows/download_pipeline.yml | 86 +++++++++++++++++++++++++ 2 files changed, 87 insertions(+), 1 deletion(-) create mode 100644 .github/workflows/download_pipeline.yml diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 124eab4..3051872 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -31,7 +31,7 @@ jobs: uses: actions/checkout@v3 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 with: version: "${{ matrix.NXF_VER }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml new file mode 100644 index 0000000..2d20d64 --- /dev/null +++ b/.github/workflows/download_pipeline.yml @@ -0,0 +1,86 @@ +name: Test successful pipeline download with 'nf-core download' + +# Run the workflow when: +# - dispatched manually +# - when a PR is opened or reopened to master branch +# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. +on: + workflow_dispatch: + inputs: + testbranch: + description: "The specific branch you wish to utilize for the test execution of nf-core download." + required: true + default: "dev" + pull_request: + types: + - opened + - edited + - synchronize + branches: + - master + pull_request_target: + branches: + - master + +env: + NXF_ANSI_LOG: false + +jobs: + download: + runs-on: ubuntu-latest + steps: + - name: Install Nextflow + uses: nf-core/setup-nextflow@v2 + + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 + with: + python-version: "3.12" + architecture: "x64" + - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 + with: + singularity-version: 3.8.3 + + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install git+https://github.com/nf-core/tools.git@dev + + - name: Get the repository name and current branch set as environment variable + run: | + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} + + - name: Download the pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + run: | + nf-core download ${{ env.REPO_LOWERCASE }} \ + --revision ${{ env.REPO_BRANCH }} \ + --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ + --compress "none" \ + --container-system 'singularity' \ + --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-cache-utilisation 'amend' \ + --download-configuration + + - name: Inspect download + run: tree ./${{ env.REPOTITLE_LOWERCASE }} + + - name: Run the downloaded pipeline (stub) + id: stub_run_pipeline + continue-on-error: true + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + - name: Run the downloaded pipeline (stub run not supported) + id: run_pipeline + if: ${{ job.steps.stub_run_pipeline.status == failure() }} + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results From aa800f8ac735f8c6d97d0d92fbb15184c65d176c Mon Sep 17 00:00:00 2001 From: reichan1998 Date: Mon, 22 Jul 2024 20:17:28 +0700 Subject: [PATCH 074/111] add a FASTQ sample into samplesheet_s3.csv for test profile --- assets/samplesheet_s3.csv | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/assets/samplesheet_s3.csv b/assets/samplesheet_s3.csv index 5089aed..b409060 100644 --- a/assets/samplesheet_s3.csv +++ b/assets/samplesheet_s3.csv @@ -1,6 +1,6 @@ sample,datatype,datafile,library mMelMel1,illumina,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel1/illumina/31231_3%231.subset.cram, -mMelMel2,illumina,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel2/illumina/31231_4%231.subset.cram, +mMelMel2,illumina,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel2/illumina/31231_4%231.subset.fastq.gz, mMelMel3,hic,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel3/hic/35528_2%231.subset.cram, mMelMel3,ont,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel3/ont/PAE35587_pass_1f1f0707_115.subset.fastq.gz, mMelMel3,pacbio,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel3/pacbio/m64094_200910_173211.ccs.bc1022_BAK8B_OA--bc1022_BAK8B_OA.subset.bam, From 8791fec9a9d1e316395d2b1b2ebe1f0606a82431 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 14:42:59 +0100 Subject: [PATCH 075/111] python black linting --- pyproject.toml | 10 ++++++++++ workflows/readmapping.nf | 2 +- 2 files changed, 11 insertions(+), 1 deletion(-) create mode 100644 pyproject.toml diff --git a/pyproject.toml b/pyproject.toml new file mode 100644 index 0000000..0d62beb --- /dev/null +++ b/pyproject.toml @@ -0,0 +1,10 @@ +# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. +[tool.black] +line-length = 120 +target_version = ["py37", "py38", "py39", "py310"] + +[tool.isort] +profile = "black" +known_first_party = ["nf_core"] +multi_line_output = 3 diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index 466ef18..b90f244 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -58,7 +58,7 @@ workflow READMAPPING { ch_multiqc_files = Channel.empty() // - // MODULE: Run FastQC + // SUBWORKFLOW: Read in samplesheet, validate and stage input files // INPUT_CHECK ( ch_samplesheet ).reads | branch { From c7693260943b1ae3abe5a6a2065b5261469063f8 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 14:45:36 +0100 Subject: [PATCH 076/111] nf-core linting --- .nf-core.yml | 23 +++++++++++++++++++++++ 1 file changed, 23 insertions(+) diff --git a/.nf-core.yml b/.nf-core.yml index 770a543..4e7854c 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -2,3 +2,26 @@ nf_core_version: 2.14.1 repository_type: pipeline template: name: readmapping +lint: + files_exist: + - assets/multiqc_config.yml + - assets/nf-core-readmapping_logo_light.png + - conf/igenomes.config + - docs/images/nf-core-readmapping_logo_dark.png + - docs/images/nf-core-readmapping_logo_light.png + - .github/ISSUE_TEMPLATE/config.yml + - .github/workflows/awstest.yml + - .github/workflows/awsfulltest.yml + files_unchanged: + - LICENSE + - .github/CONTRIBUTING.md + - .github/ISSUE_TEMPLATE/bug_report.yml + - .github/workflows/linting_comment.yml + - assets/email_template.html + - assets/sendmail_template.txt + - docs/README.md + - lib/NfcoreTemplate.groovy + - .github/workflows/branch.yml + nextflow_config: + - manifest.name + - manifest.homePage From 6660c198a9b6e69c71da09a89463a2456aaaaf8d Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 15:23:14 +0100 Subject: [PATCH 077/111] nf-core linting --- .nf-core.yml | 4 ++++ assets/multiqc_config.yml | 17 ----------------- 2 files changed, 4 insertions(+), 17 deletions(-) delete mode 100644 assets/multiqc_config.yml diff --git a/.nf-core.yml b/.nf-core.yml index 4e7854c..768833a 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -22,6 +22,10 @@ lint: - docs/README.md - lib/NfcoreTemplate.groovy - .github/workflows/branch.yml + - .gitignore + - .github/workflows/linting.yml + - .github/PULL_REQUEST_TEMPLATE.md nextflow_config: - manifest.name - manifest.homePage + multiqc_config: False diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml deleted file mode 100644 index fd62d13..0000000 --- a/assets/multiqc_config.yml +++ /dev/null @@ -1,17 +0,0 @@ -report_comment: > - - This report has been generated by the sanger-tol/readmapping - analysis pipeline. For information about how to interpret these results, please see the - documentation. - -report_section_order: - "sanger-tol-readmapping-methods-description": - order: -1000 - software_versions: - order: -1001 - "sanger-tol-readmapping-summary": - order: -1002 - -export_plots: true - -disable_version_detection: true From fa3ae155072d811e251a1c58d83088a0f767c2f6 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 15:26:42 +0100 Subject: [PATCH 078/111] nf-core linting --- .github/workflows/linting.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 858d622..84b951f 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -74,7 +74,7 @@ jobs: uses: actions/checkout@v3 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 - uses: actions/setup-python@v4 with: From 6d241115dc1eb4a77a4936f0a4ee442a999e76cb Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 22 Jul 2024 15:33:28 +0100 Subject: [PATCH 079/111] nf-core linting --- .github/workflows/download_pipeline.yml | 6 ++++-- .github/workflows/linting.yml | 2 +- 2 files changed, 5 insertions(+), 3 deletions(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 2d20d64..bd9f7bf 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -17,10 +17,12 @@ on: - edited - synchronize branches: - - master + - main + - dev pull_request_target: branches: - - master + - main + - dev env: NXF_ANSI_LOG: false diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 84b951f..2438998 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -84,7 +84,7 @@ jobs: - name: Install dependencies run: | python -m pip install --upgrade pip - pip install nf-core + pip install nf-core==2.14 - name: Run nf-core lint env: From 161f3f13bbf6594250fc291483515fe7dc136556 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Tue, 23 Jul 2024 11:00:18 +0100 Subject: [PATCH 080/111] nf-core linting --- .github/workflows/linting.yml | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 2438998..177172b 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -78,13 +78,13 @@ jobs: - uses: actions/setup-python@v4 with: - python-version: "3.7" + python-version: "3.10" architecture: "x64" - name: Install dependencies run: | python -m pip install --upgrade pip - pip install nf-core==2.14 + pip install nf-core==2.14.1 - name: Run nf-core lint env: From 797099e9bc5cdbab0ebf643fd1109bde26d44246 Mon Sep 17 00:00:00 2001 From: reichan1998 Date: Thu, 25 Jul 2024 00:53:31 +0700 Subject: [PATCH 081/111] add function to check datatype --- subworkflows/local/prepare_genome.nf | 34 +++++++++++++++++++++------- workflows/readmapping.nf | 15 +----------- 2 files changed, 27 insertions(+), 22 deletions(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 0fe5a8b..9f51967 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -11,7 +11,6 @@ include { BWAMEM2_INDEX } from '../../modules/nf-core/bwamem2/index/main' workflow PREPARE_GENOME { take: fasta // channel: [ meta, /path/to/fasta ] - short_reads // channel: [ meta, /path/to/reads ] main: ch_versions = Channel.empty() @@ -33,12 +32,8 @@ workflow PREPARE_GENOME { UNMASK ( ch_fasta ) ch_versions = ch_versions.mix ( UNMASK.out.versions ) - // def align_short = short_reads ? true : false - // Generate BWA index - ch_bwamem2_index = Channel.empty() - - if ( short_reads ) { + if ( checkShortReads(params.input) ) { if ( params.bwamem2_index ) { Channel.fromPath ( params.bwamem2_index ) | combine ( ch_fasta ) @@ -47,15 +42,17 @@ workflow PREPARE_GENOME { if ( params.bwamem2_index.endsWith('.tar.gz') ) { ch_bwamem2_index = UNTAR ( ch_bwamem ).untar - ch_versions = ch_versions.mix ( UNTAR.out.versions ) + ch_versions = ch_versions.mix ( UNTAR.out.versions.first() ) } else { ch_bwamem2_index = ch_bwamem } } else { ch_bwamem2_index = BWAMEM2_INDEX ( UNMASK.out.fasta ).index - ch_versions = ch_versions.mix ( BWAMEM2_INDEX.out.versions ) + ch_versions = ch_versions.mix ( BWAMEM2_INDEX.out.versions.first() ) } + } else { + ch_bwamem2_index = Channel.empty() } @@ -64,3 +61,24 @@ workflow PREPARE_GENOME { bwaidx = ch_bwamem2_index.first() // channel: [ meta, /path/to/bwamem2/index_dir/ ] versions = ch_versions // channel: [ versions.yml ] } + +def checkShortReads(filePath, columnToCheck="datatype") { + // Define the target values to check + def valuesToCheck = ['illumina', 'hic'] + // Read the CSV file + def csvLines = new File(filePath).readLines() + // Extract the header and find the index of the column + def header = csvLines[0].split(',') + def columnIndex = header.findIndexOf { it == columnToCheck } + // Check if the column index was found + if (columnIndex == -1) { + println "Column '${columnToCheck}' not found in the CSV header." + return false + } + // Check for the values in the specified column and return true if found + def containsValues = csvLines[1..-1].any { line -> + def columns = line.split(',') + valuesToCheck.contains(columns[columnIndex].toLowerCase()) + } + return containsValues +} \ No newline at end of file diff --git a/workflows/readmapping.nf b/workflows/readmapping.nf index 043c745..32c0805 100644 --- a/workflows/readmapping.nf +++ b/workflows/readmapping.nf @@ -75,25 +75,12 @@ workflow READMAPPING { | set { ch_reads } ch_versions = ch_versions.mix ( INPUT_CHECK.out.versions ) - // Determine datatype of short and long reads - INPUT_CHECK.out.reads - | branch { - meta, reads -> - short_reads: meta.datatype == "illumina" || meta.datatype == "hic" - return [meta.datatype, true] - long_reads: meta.datatype == "pacbio" || meta.datatype == "pacbio_clr" || meta.datatype == "ont" - return [meta.datatype, false] - } - | set { ch_filtered_reads } - // - // SUBWORKFLOW: Uncompress and prepare reference genome files - // ch_fasta | map { [ [ id: it.baseName ], it ] } | set { ch_genome } - PREPARE_GENOME ( ch_genome, ch_filtered_reads.short_reads ) + PREPARE_GENOME ( ch_genome ) ch_versions = ch_versions.mix ( PREPARE_GENOME.out.versions ) From 26589a64fd84ea09036685e74d85429c46b215c1 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Sun, 28 Jul 2024 11:23:08 +0100 Subject: [PATCH 082/111] nf-core lint --- .nf-core.yml | 2 ++ 1 file changed, 2 insertions(+) diff --git a/.nf-core.yml b/.nf-core.yml index 768833a..24476e6 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -28,4 +28,6 @@ lint: nextflow_config: - manifest.name - manifest.homePage + - config_defaults: + - params.vector_db multiqc_config: False From 77b885244c9f9adc37cecd0be568dd14ff8e9e0d Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Sun, 28 Jul 2024 11:28:22 +0100 Subject: [PATCH 083/111] prettier lint --- .nf-core.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.nf-core.yml b/.nf-core.yml index 24476e6..9492a1b 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -29,5 +29,5 @@ lint: - manifest.name - manifest.homePage - config_defaults: - - params.vector_db + - params.vector_db multiqc_config: False From 3ffb04234deeccf0323f3474c1823b98024c8563 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 31 Jul 2024 15:12:06 +0100 Subject: [PATCH 084/111] revert projectDir change in nextflow_schema.json --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index bc6ef19..618c3d0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -33,7 +33,7 @@ "type": "string", "description": "Path to directory or tar.gz archive for pre-built PacBio vector database.", "format": "path", - "default": "/Users/tc25/projects/readmapping/assets/vectorDB.tar.gz", + "default": "${projectDir}/assets/vectorDB.tar.gz", "fa_icon": "fas fa-bezier-curve" }, "email": { From 9bc900975f3d3065ae22b3de87f37d6bf5a4212a Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Thu, 1 Aug 2024 12:40:07 +0100 Subject: [PATCH 085/111] outdir for test profile --- conf/test.config | 3 +++ 1 file changed, 3 insertions(+) diff --git a/conf/test.config b/conf/test.config index e269f68..d78e008 100644 --- a/conf/test.config +++ b/conf/test.config @@ -22,6 +22,9 @@ params { // Input data input = "${projectDir}/assets/samplesheet_s3.csv" + // Output directory + outdir = "${projectDir}/results" + // Fasta references fasta = "https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/assembly/release/mMelMel3.1_paternal_haplotype/GCA_922984935.2.subset.fasta.gz" header = "https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/assembly/release/mMelMel3.1_paternal_haplotype/GCA_922984935.2.subset.header.sam" From 816bd467872f62825a9bbb544f172e4c305203f7 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Thu, 1 Aug 2024 12:40:33 +0100 Subject: [PATCH 086/111] outdir for test profile --- conf/test_full.config | 3 +++ 1 file changed, 3 insertions(+) diff --git a/conf/test_full.config b/conf/test_full.config index 562b16d..ddfcacd 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -17,6 +17,9 @@ params { // Input data for full size test input = "${projectDir}/assets/samplesheet_full.csv" + // Output directory + outdir = "${projectDir}/results" + // Fasta references fasta = "/lustre/scratch124/tol/projects/darwin/data/insects/Polyommatus_icarus/assembly/release/ilPolIcar1.1/insdc/GCA_937595015.1.fasta.gz" From d4f79022f1a5898efc07bc8cc9f6d182249db129 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 2 Aug 2024 08:33:16 +0100 Subject: [PATCH 087/111] revert samplesheet change --- assets/samplesheet_s3.csv | 3 +++ 1 file changed, 3 insertions(+) diff --git a/assets/samplesheet_s3.csv b/assets/samplesheet_s3.csv index a78ba9a..b409060 100644 --- a/assets/samplesheet_s3.csv +++ b/assets/samplesheet_s3.csv @@ -1,4 +1,7 @@ sample,datatype,datafile,library +mMelMel1,illumina,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel1/illumina/31231_3%231.subset.cram, +mMelMel2,illumina,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel2/illumina/31231_4%231.subset.fastq.gz, +mMelMel3,hic,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel3/hic/35528_2%231.subset.cram, mMelMel3,ont,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel3/ont/PAE35587_pass_1f1f0707_115.subset.fastq.gz, mMelMel3,pacbio,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel3/pacbio/m64094_200910_173211.ccs.bc1022_BAK8B_OA--bc1022_BAK8B_OA.subset.bam, mMelMel3,pacbio,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel3/pacbio/m64094_200911_174739.ccs.bc1022_BAK8B_OA--bc1022_BAK8B_OA.subset.bam, From 77f45678ed3fe122784ddb2c81e83fc65314988f Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Fri, 2 Aug 2024 13:24:25 +0100 Subject: [PATCH 088/111] test --- .nf-core.yml | 1 + 1 file changed, 1 insertion(+) diff --git a/.nf-core.yml b/.nf-core.yml index 1adc147..6d9e4e4 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -2,6 +2,7 @@ nf_core_version: 2.14.1 repository_type: pipeline template: name: readmapping + prefix: sanger-tol lint: files_exist: - assets/multiqc_config.yml From 6ea89d6421763ba7c23c374174cb32d66028cc44 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 7 Aug 2024 12:10:31 +0100 Subject: [PATCH 089/111] input sanitation --- .../local/utils_nfcore_readmapping_pipeline/main.nf | 13 +++++++++++-- 1 file changed, 11 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf index cbae3b8..abfed43 100644 --- a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf @@ -181,11 +181,20 @@ def validateInputParameters() { def validateInputSamplesheet(channel) { def seen = [:].withDefault { 0 } def uniquePairs = new HashSet() + def validFormats = [".fq.gz", ".fastq.gz", ".cram", ".bam"] - // Use map to process each item in the channel return channel.map { sample -> def (meta, file) = sample - def pair = [meta.sample, meta.datafile].toString() + + // Replace spaces with underscores in sample names + meta.sample = meta.sample.replace(" ", "_") + + // Validate that the file path is non-empty and has a valid format + if (!file || !validFormats.any { file.toString().endsWith(it) }) { + error("Data file is required and must have a valid extension: ${file}") + } + + def pair = [meta.sample, file.toString()].toString() if (!uniquePairs.add(pair)) { error("The pair of sample name and read file must be unique: ${pair}") From b8f41677cb6d596ed58738ebcfea7ba61e0f0113 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 7 Aug 2024 12:11:22 +0100 Subject: [PATCH 090/111] fix issue with resuming pipeline caused by meta.file input type --- assets/schema_input.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index 16f0f28..d1c2473 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -20,7 +20,7 @@ "meta": ["datatype"] }, "datafile": { - "format": "file-path", + "format": "string", "exists": true, "pattern": "^\\S+$", "errorMessage": "Data file for reads cannot contain spaces and must have extension 'cram', 'bam', '.fq.gz' or '.fastq.gz'", From fbcaf1ebfc0808e9902a62d31f8bf75edf6af654 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 7 Aug 2024 12:11:25 +0100 Subject: [PATCH 091/111] fix issue with resuming pipeline caused by meta.file input type --- assets/schema_input.json | 1 - 1 file changed, 1 deletion(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index d1c2473..c1197d7 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -21,7 +21,6 @@ }, "datafile": { "format": "string", - "exists": true, "pattern": "^\\S+$", "errorMessage": "Data file for reads cannot contain spaces and must have extension 'cram', 'bam', '.fq.gz' or '.fastq.gz'", "meta": ["datafile"] From 4e220da4e75c2dc0f0918598a1022c4d6370c4a1 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 7 Aug 2024 12:56:47 +0100 Subject: [PATCH 092/111] remove debug print --- subworkflows/local/prepare_genome.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index d0e4dd6..aa951b6 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -86,7 +86,7 @@ def checkShortReads(filePath, columnToCheck="datatype") { def columns = line.split(',') valuesToCheck.contains(columns[columnIndex].toLowerCase()) } - println(containsValues) + return containsValues } From 8d32f1f0652eafe2107776417277b904d6b5c01e Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 14 Aug 2024 11:18:16 +0100 Subject: [PATCH 093/111] nf-core modules install seqkit/fq2fa --- modules.json | 5 ++ modules/nf-core/seqkit/fq2fa/environment.yml | 7 ++ modules/nf-core/seqkit/fq2fa/main.nf | 48 +++++++++++++ modules/nf-core/seqkit/fq2fa/meta.yml | 44 ++++++++++++ .../nf-core/seqkit/fq2fa/tests/main.nf.test | 56 +++++++++++++++ .../seqkit/fq2fa/tests/main.nf.test.snap | 72 +++++++++++++++++++ modules/nf-core/seqkit/fq2fa/tests/tags.yml | 2 + 7 files changed, 234 insertions(+) create mode 100644 modules/nf-core/seqkit/fq2fa/environment.yml create mode 100644 modules/nf-core/seqkit/fq2fa/main.nf create mode 100644 modules/nf-core/seqkit/fq2fa/meta.yml create mode 100644 modules/nf-core/seqkit/fq2fa/tests/main.nf.test create mode 100644 modules/nf-core/seqkit/fq2fa/tests/main.nf.test.snap create mode 100644 modules/nf-core/seqkit/fq2fa/tests/tags.yml diff --git a/modules.json b/modules.json index 113030b..60d80ef 100644 --- a/modules.json +++ b/modules.json @@ -86,6 +86,11 @@ "git_sha": "6c2309aaec566c0d44a6cf14d4b2d0c51afe2e91", "installed_by": ["modules"] }, + "seqkit/fq2fa": { + "branch": "master", + "git_sha": "03fbf6c89e551bd8d77f3b751fb5c955f75b34c5", + "installed_by": ["modules"] + }, "untar": { "branch": "master", "git_sha": "4e5f4687318f24ba944a13609d3ea6ebd890737d", diff --git a/modules/nf-core/seqkit/fq2fa/environment.yml b/modules/nf-core/seqkit/fq2fa/environment.yml new file mode 100644 index 0000000..aede676 --- /dev/null +++ b/modules/nf-core/seqkit/fq2fa/environment.yml @@ -0,0 +1,7 @@ +name: seqkit_fq2fa +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::seqkit=2.8.1 diff --git a/modules/nf-core/seqkit/fq2fa/main.nf b/modules/nf-core/seqkit/fq2fa/main.nf new file mode 100644 index 0000000..77462ad --- /dev/null +++ b/modules/nf-core/seqkit/fq2fa/main.nf @@ -0,0 +1,48 @@ +process SEQKIT_FQ2FA { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/seqkit:2.8.1--h9ee0642_0' : + 'biocontainers/seqkit:2.8.1--h9ee0642_0' }" + + input: + tuple val(meta), path(fastq) + + output: + tuple val(meta), path("*.fa.gz"), emit: fasta + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + seqkit \\ + fq2fa \\ + $args \\ + -j $task.cpus \\ + -o ${prefix}.fa.gz \\ + $fastq + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + seqkit: \$( seqkit | sed '3!d; s/Version: //' ) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + echo "" | gzip > ${prefix}.fa.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + seqkit: \$( seqkit | sed '3!d; s/Version: //' ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/seqkit/fq2fa/meta.yml b/modules/nf-core/seqkit/fq2fa/meta.yml new file mode 100644 index 0000000..d0c55b3 --- /dev/null +++ b/modules/nf-core/seqkit/fq2fa/meta.yml @@ -0,0 +1,44 @@ +name: "seqkit_fq2fa" +description: Convert FASTQ to FASTA format +keywords: + - fastq + - fasta + - convert +tools: + - "seqkit": + description: "Cross-platform and ultrafast toolkit for FASTA/Q file manipulation, written by Wei Shen." + homepage: "https://github.com/shenwei356/seqkit" + documentation: "https://bioinf.shenwei.me/seqkit/" + doi: "10.1371/journal.pone.0163962" + licence: ["MIT"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + + - fastq: + type: file + description: Sequence file in fastq format + pattern: "*.{fastq,fq}.gz" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - fasta: + type: file + description: Sequence file in fasta format + pattern: "*.{fasta,fa}.gz" + +authors: + - "@d-jch" diff --git a/modules/nf-core/seqkit/fq2fa/tests/main.nf.test b/modules/nf-core/seqkit/fq2fa/tests/main.nf.test new file mode 100644 index 0000000..08f399e --- /dev/null +++ b/modules/nf-core/seqkit/fq2fa/tests/main.nf.test @@ -0,0 +1,56 @@ +nextflow_process { + + name "Test Process SEQKIT_FQ2FA" + script "../main.nf" + process "SEQKIT_FQ2FA" + + tag "modules" + tag "modules_nfcore" + tag "seqkit" + tag "seqkit/fq2fa" + + test("sarscov2 - bam") { + + when { + process { + """ + input[0] = [[ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - bam - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [[ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/seqkit/fq2fa/tests/main.nf.test.snap b/modules/nf-core/seqkit/fq2fa/tests/main.nf.test.snap new file mode 100644 index 0000000..b10ff75 --- /dev/null +++ b/modules/nf-core/seqkit/fq2fa/tests/main.nf.test.snap @@ -0,0 +1,72 @@ +{ + "sarscov2 - bam - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.fa.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + "versions.yml:md5,70efc6839fd6443ee9116c082a730f72" + ], + "fasta": [ + [ + { + "id": "test", + "single_end": false + }, + "test.fa.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,70efc6839fd6443ee9116c082a730f72" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-13T08:56:21.234724552" + }, + "sarscov2 - bam": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.fa.gz:md5,f0c5c9110ce19e9ebbc9a6b6baf9e105" + ] + ], + "1": [ + "versions.yml:md5,70efc6839fd6443ee9116c082a730f72" + ], + "fasta": [ + [ + { + "id": "test", + "single_end": false + }, + "test.fa.gz:md5,f0c5c9110ce19e9ebbc9a6b6baf9e105" + ] + ], + "versions": [ + "versions.yml:md5,70efc6839fd6443ee9116c082a730f72" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-13T08:55:54.648865102" + } +} \ No newline at end of file diff --git a/modules/nf-core/seqkit/fq2fa/tests/tags.yml b/modules/nf-core/seqkit/fq2fa/tests/tags.yml new file mode 100644 index 0000000..004f102 --- /dev/null +++ b/modules/nf-core/seqkit/fq2fa/tests/tags.yml @@ -0,0 +1,2 @@ +seqkit/fq2fa: + - "modules/nf-core/seqkit/fq2fa/**" From 33b0247d3ec475298bcc01d60937aa881ecbc50e Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 14 Aug 2024 11:30:28 +0100 Subject: [PATCH 094/111] nf-core modules install seqtk/subseq --- modules.json | 5 ++ modules/nf-core/seqtk/subseq/environment.yml | 7 +++ modules/nf-core/seqtk/subseq/main.nf | 56 +++++++++++++++++ modules/nf-core/seqtk/subseq/meta.yml | 40 +++++++++++++ .../nf-core/seqtk/subseq/tests/main.nf.test | 59 ++++++++++++++++++ .../seqtk/subseq/tests/main.nf.test.snap | 60 +++++++++++++++++++ .../seqtk/subseq/tests/standard.config | 5 ++ modules/nf-core/seqtk/subseq/tests/tags.yml | 2 + 8 files changed, 234 insertions(+) create mode 100644 modules/nf-core/seqtk/subseq/environment.yml create mode 100644 modules/nf-core/seqtk/subseq/main.nf create mode 100644 modules/nf-core/seqtk/subseq/meta.yml create mode 100644 modules/nf-core/seqtk/subseq/tests/main.nf.test create mode 100644 modules/nf-core/seqtk/subseq/tests/main.nf.test.snap create mode 100644 modules/nf-core/seqtk/subseq/tests/standard.config create mode 100644 modules/nf-core/seqtk/subseq/tests/tags.yml diff --git a/modules.json b/modules.json index 60d80ef..0653c8f 100644 --- a/modules.json +++ b/modules.json @@ -91,6 +91,11 @@ "git_sha": "03fbf6c89e551bd8d77f3b751fb5c955f75b34c5", "installed_by": ["modules"] }, + "seqtk/subseq": { + "branch": "master", + "git_sha": "730f3aee80d5f8d0b5fc532202ac59361414d006", + "installed_by": ["modules"] + }, "untar": { "branch": "master", "git_sha": "4e5f4687318f24ba944a13609d3ea6ebd890737d", diff --git a/modules/nf-core/seqtk/subseq/environment.yml b/modules/nf-core/seqtk/subseq/environment.yml new file mode 100644 index 0000000..7abe364 --- /dev/null +++ b/modules/nf-core/seqtk/subseq/environment.yml @@ -0,0 +1,7 @@ +name: seqtk_subseq +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::seqtk=1.4 diff --git a/modules/nf-core/seqtk/subseq/main.nf b/modules/nf-core/seqtk/subseq/main.nf new file mode 100644 index 0000000..d5caebc --- /dev/null +++ b/modules/nf-core/seqtk/subseq/main.nf @@ -0,0 +1,56 @@ +process SEQTK_SUBSEQ { + tag "$sequences" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/seqtk:1.4--he4a0461_1' : + 'biocontainers/seqtk:1.4--he4a0461_1' }" + + input: + tuple val(meta), path(sequences) + path filter_list + + output: + tuple val(meta), path("*.gz"), emit: sequences + path "versions.yml", emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def ext = "fa" + if ("$sequences" ==~ /.+\.fq|.+\.fq.gz|.+\.fastq|.+\.fastq.gz/) { + ext = "fq" + } + """ + seqtk \\ + subseq \\ + $args \\ + $sequences \\ + $filter_list | \\ + gzip --no-name > ${sequences}${prefix}.${ext}.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + seqtk: \$(echo \$(seqtk 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def ext = "fa" + if ("$sequences" ==~ /.+\.fq|.+\.fq.gz|.+\.fastq|.+\.fastq.gz/) { + ext = "fq" + } + """ + echo "" | gzip > ${sequences}${prefix}.${ext}.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + seqtk: \$(echo \$(seqtk 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/seqtk/subseq/meta.yml b/modules/nf-core/seqtk/subseq/meta.yml new file mode 100644 index 0000000..de4a841 --- /dev/null +++ b/modules/nf-core/seqtk/subseq/meta.yml @@ -0,0 +1,40 @@ +name: seqtk_subseq +description: Select only sequences that match the filtering condition +keywords: + - filtering + - selection + - fastx +tools: + - seqtk: + description: Seqtk is a fast and lightweight tool for processing sequences in the FASTA or FASTQ format + homepage: https://github.com/lh3/seqtk + documentation: https://docs.csc.fi/apps/seqtk/ + tool_dev_url: https://github.com/lh3/seqtk + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test' ] + - sequences: + type: file + description: FASTQ/FASTA file + pattern: "*.{fq,fq.gz,fa,fa.gz}" + - filter_list: + type: file + description: BED file or a text file with a list of sequence names + pattern: "*.{bed,lst}" +output: + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - sequences: + type: file + description: FASTQ/FASTA file + pattern: "*.{fq.gz,fa.gz}" +authors: + - "@sidorov-si" +maintainers: + - "@sidorov-si" diff --git a/modules/nf-core/seqtk/subseq/tests/main.nf.test b/modules/nf-core/seqtk/subseq/tests/main.nf.test new file mode 100644 index 0000000..fa8fad6 --- /dev/null +++ b/modules/nf-core/seqtk/subseq/tests/main.nf.test @@ -0,0 +1,59 @@ +nextflow_process { + + name "Test Process SEQTK_SUBSEQ" + script "modules/nf-core/seqtk/subseq/main.nf" + process "SEQTK_SUBSEQ" + config "./standard.config" + + tag "modules" + tag "modules_nfcore" + tag "seqtk" + tag "seqtk/subseq" + + test("sarscov2_subseq_fa") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[1] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed.gz', checkIfExists: true) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2_subseq_fa_stub") { + options "-stub" + when { + process { + """ + input[0] = [ + [ id:'test' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[1] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed.gz', checkIfExists: true) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/seqtk/subseq/tests/main.nf.test.snap b/modules/nf-core/seqtk/subseq/tests/main.nf.test.snap new file mode 100644 index 0000000..75b3793 --- /dev/null +++ b/modules/nf-core/seqtk/subseq/tests/main.nf.test.snap @@ -0,0 +1,60 @@ +{ + "sarscov2_subseq_fa": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "genome.fasta.filtered.fa.gz:md5,31c95c4d686526cf002f6119bc55b2b2" + ] + ], + "1": [ + "versions.yml:md5,cd7682f4da748ef6d083c4a4656cc1e2" + ], + "sequences": [ + [ + { + "id": "test" + }, + "genome.fasta.filtered.fa.gz:md5,31c95c4d686526cf002f6119bc55b2b2" + ] + ], + "versions": [ + "versions.yml:md5,cd7682f4da748ef6d083c4a4656cc1e2" + ] + } + ], + "timestamp": "2024-02-22T15:56:36.155954" + }, + "sarscov2_subseq_fa_stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "genome.fasta.filtered.fa.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + "versions.yml:md5,cd7682f4da748ef6d083c4a4656cc1e2" + ], + "sequences": [ + [ + { + "id": "test" + }, + "genome.fasta.filtered.fa.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,cd7682f4da748ef6d083c4a4656cc1e2" + ] + } + ], + "timestamp": "2024-02-22T15:56:44.222329" + } +} \ No newline at end of file diff --git a/modules/nf-core/seqtk/subseq/tests/standard.config b/modules/nf-core/seqtk/subseq/tests/standard.config new file mode 100644 index 0000000..e8d7dc3 --- /dev/null +++ b/modules/nf-core/seqtk/subseq/tests/standard.config @@ -0,0 +1,5 @@ +process { + withName: SEQTK_SUBSEQ { + ext.prefix = { ".filtered" } + } +} \ No newline at end of file diff --git a/modules/nf-core/seqtk/subseq/tests/tags.yml b/modules/nf-core/seqtk/subseq/tests/tags.yml new file mode 100644 index 0000000..74056ba --- /dev/null +++ b/modules/nf-core/seqtk/subseq/tests/tags.yml @@ -0,0 +1,2 @@ +seqtk/subseq: + - "modules/nf-core/seqtk/subseq/**" From dda1b1c4264229bc183be0baf5b18c19d5292501 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 14 Aug 2024 14:34:31 +0100 Subject: [PATCH 095/111] added fastq option for pacbio input --- assets/samplesheet_s3.csv | 2 +- docs/usage.md | 2 +- subworkflows/local/filter_pacbio.nf | 66 +++++++++++++++++++++++------ 3 files changed, 54 insertions(+), 16 deletions(-) diff --git a/assets/samplesheet_s3.csv b/assets/samplesheet_s3.csv index b409060..ff9641b 100644 --- a/assets/samplesheet_s3.csv +++ b/assets/samplesheet_s3.csv @@ -4,4 +4,4 @@ mMelMel2,illumina,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_d mMelMel3,hic,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel3/hic/35528_2%231.subset.cram, mMelMel3,ont,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel3/ont/PAE35587_pass_1f1f0707_115.subset.fastq.gz, mMelMel3,pacbio,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel3/pacbio/m64094_200910_173211.ccs.bc1022_BAK8B_OA--bc1022_BAK8B_OA.subset.bam, -mMelMel3,pacbio,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel3/pacbio/m64094_200911_174739.ccs.bc1022_BAK8B_OA--bc1022_BAK8B_OA.subset.bam, +mMelMel3,pacbio,https://tolit.cog.sanger.ac.uk/test-data/Meles_meles/genomic_data/mMelMel3/pacbio/m64094_200911_174739.ccs.bc1022_BAK8B_OA--bc1022_BAK8B_OA.subset.fastq.gz, diff --git a/docs/usage.md b/docs/usage.md index 9a02085..2e707a9 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -42,7 +42,7 @@ sample1_T5,pacbio,pacbio2.bam,pacbio2 | ---------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (\_). | | `datatype` | Type of sequencing data. Must be one of `hic`, `Illumina`, `pacbio`, or `ont`. | -| `datafile` | Full path to read data file. Must be `bam` or `cram` or `fastq.gz` or `fq.gz` for `Illumina` and `HiC`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | +| `datafile` | Full path to read data file. Must be `bam`, `cram`, `fastq.gz` or `fq.gz` for `Illumina` and `HiC`. Must be `bam`, `fastq.gz` or `fq.gz` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | | `library` | (Optional) The library value is a unique identifier which is assigned to read group (`@RG`) ID. If the library name is not specified, the pipeline will auto-create library name using the data filename provided in the samplesheet. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. diff --git a/subworkflows/local/filter_pacbio.nf b/subworkflows/local/filter_pacbio.nf index 759880c..acb21fa 100644 --- a/subworkflows/local/filter_pacbio.nf +++ b/subworkflows/local/filter_pacbio.nf @@ -8,6 +8,8 @@ include { SAMTOOLS_COLLATETOFASTA } from '../../modules/local/samtools include { BLAST_BLASTN } from '../../modules/nf-core/blast/blastn/main' include { PACBIO_FILTER } from '../../modules/local/pacbio_filter' include { SAMTOOLS_FILTERTOFASTQ } from '../../modules/local/samtools_filtertofastq' +include { SEQKIT_FQ2FA } from '../../modules/nf-core/seqkit/fq2fa' +include { SEQTK_SUBSEQ } from '../../modules/nf-core/seqtk/subseq' workflow FILTER_PACBIO { @@ -20,8 +22,18 @@ workflow FILTER_PACBIO { ch_versions = Channel.empty() - // Convert from PacBio BAM to Samtools BAM + // Check file types and branch reads + | branch { + meta, reads -> + fastq : reads.findAll { it.getName().toLowerCase() =~ /.*f.*\.gz/ } + bam : true + } + | set { ch_reads } + + + // Convert from PacBio BAM to Samtools BAM + ch_reads.bam | map { meta, bam -> [ meta, bam, [] ] } | set { ch_pacbio } @@ -34,8 +46,19 @@ workflow FILTER_PACBIO { ch_versions = ch_versions.mix ( SAMTOOLS_COLLATETOFASTA.out.versions.first() ) + // Convert FASTQ to FASTA using SEQKIT_FQ2FA + SEQKIT_FQ2FA ( ch_reads.fastq ) + ch_versions = ch_versions.mix ( SEQKIT_FQ2FA.out.versions.first() ) + + + // Combine BAM-derived FASTA with converted FASTQ inputs + SAMTOOLS_COLLATETOFASTA.out.fasta + | concat( SEQKIT_FQ2FA.out.fasta ) + | set { ch_fasta } + + // Nucleotide BLAST - BLAST_BLASTN ( SAMTOOLS_COLLATETOFASTA.out.fasta, db ) + BLAST_BLASTN ( ch_fasta, db ) ch_versions = ch_versions.mix ( BLAST_BLASTN.out.versions.first() ) @@ -44,25 +67,40 @@ workflow FILTER_PACBIO { ch_versions = ch_versions.mix ( PACBIO_FILTER.out.versions.first() ) - // Filter the BAM file and convert to FASTQ + // Filter the BAM files and convert to FASTQ SAMTOOLS_CONVERT.out.bam | join ( SAMTOOLS_CONVERT.out.csi ) | join ( PACBIO_FILTER.out.list ) - | set { ch_reads_and_list } + | multiMap { meta, bam, csi, list -> \ + bams: [meta, bam, csi] + lists: list + } + | set { ch_bam_reads } - ch_reads_and_list - | map { meta, bam, csi, list -> [meta, bam, csi] } - | set { ch_reads } + SAMTOOLS_FILTERTOFASTQ ( ch_bam_reads.bams, ch_bam_reads.lists ) + ch_versions = ch_versions.mix ( SAMTOOLS_FILTERTOFASTQ.out.versions.first() ) - ch_reads_and_list - | map { meta, bam, csi, list -> list } - | set { ch_lists } - SAMTOOLS_FILTERTOFASTQ ( ch_reads, ch_lists ) - ch_versions = ch_versions.mix ( SAMTOOLS_FILTERTOFASTQ.out.versions.first() ) + // Filter inputs provided as FASTQ + ch_reads.fastq + | join(PACBIO_FILTER.out.list) + | multiMap { meta, fastq, list -> \ + fastqs: [meta, fastq] + lists: list + } + | set { ch_reads_fastq } + + SEQTK_SUBSEQ ( ch_reads_fastq.fastqs, ch_reads_fastq.lists ) + ch_versions = ch_versions.mix ( SEQTK_SUBSEQ.out.versions.first() ) + + + // Merge filtered outputs as ch_output_fastq + SEQTK_SUBSEQ.out.sequences + | concat ( SAMTOOLS_FILTERTOFASTQ.out.fastq ) + | set { ch_filtered_fastq } emit: - fastq = SAMTOOLS_FILTERTOFASTQ.out.fastq // channel: [ meta, /path/to/fastq ] - versions = ch_versions // channel: [ versions.yml ] + fastq = ch_filtered_fastq // channel: [ meta, /path/to/fastq ] + versions = ch_versions // channel: [ versions.yml ] } From e2a8d462e54e29972c2136509e4161d22b4086a3 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 14 Aug 2024 14:39:43 +0100 Subject: [PATCH 096/111] prettier linting --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index 2e707a9..9423325 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -42,7 +42,7 @@ sample1_T5,pacbio,pacbio2.bam,pacbio2 | ---------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (\_). | | `datatype` | Type of sequencing data. Must be one of `hic`, `Illumina`, `pacbio`, or `ont`. | -| `datafile` | Full path to read data file. Must be `bam`, `cram`, `fastq.gz` or `fq.gz` for `Illumina` and `HiC`. Must be `bam`, `fastq.gz` or `fq.gz` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | +| `datafile` | Full path to read data file. Must be `bam`, `cram`, `fastq.gz` or `fq.gz` for `Illumina` and `HiC`. Must be `bam`, `fastq.gz` or `fq.gz` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | | `library` | (Optional) The library value is a unique identifier which is assigned to read group (`@RG`) ID. If the library name is not specified, the pipeline will auto-create library name using the data filename provided in the samplesheet. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. From 458cfb5245e8d965df77361e630b80c55f3b9f96 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Wed, 14 Aug 2024 17:12:46 +0100 Subject: [PATCH 097/111] added --outfmt and --compression arguments --- conf/modules.config | 7 +- docs/output.md | 30 +++-- docs/usage.md | 2 +- nextflow.config | 4 + nextflow_schema.json | 14 +- subworkflows/local/convert_stats.nf | 125 ++++++++++++------ .../utils_nfcore_readmapping_pipeline/main.nf | 20 ++- 7 files changed, 146 insertions(+), 56 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 089592e..a2d4464 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -63,7 +63,7 @@ process { ext.args = { "-ax map-ont -R ${meta.read_group} -I" + Math.ceil(meta2.genome_size/1e9) + 'G' } } - withName: '.*:CONVERT_STATS:SAMTOOLS_VIEW' { + withName: '.*:CONVERT_STATS:SAMTOOLS_CRAM' { ext.prefix = { "${fasta.baseName}.${meta.datatype}.${meta.id}" } ext.args = '--output-fmt cram --write-index' } @@ -72,6 +72,11 @@ process { ext.prefix = { "${bam.baseName}" } } + withName: SAMTOOLS_REINDEX { + ext.args = '--write-index' + ext.prefix = { "${fasta.baseName}.${meta.datatype}.${meta.id}" } + } + withName: SAMTOOLS_IDXSTATS { ext.prefix = { "${bam.baseName}" } } diff --git a/docs/output.md b/docs/output.md index e9b8b8c..c64b87b 100644 --- a/docs/output.md +++ b/docs/output.md @@ -15,6 +15,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Preprocessing](#preprocessing) - [Filtering](#filtering) – Filtering PacBio data before alignment - [Alignment and Mark duplicates](#alignment-and-mark-duplicates) + - [Output options](#outfmt) – Output options for all read types - [Short reads](#short-reads) – Aligning HiC and Illumina reads using BWAMEM2 - [Oxford Nanopore reads](#oxford-nanopore-reads) – Aligning ONT reads using MINIMAP2 - [PacBio reads](#pacbio-reads) – Aligning PacBio CLR and CCS filtered reads using MINIMAP2 @@ -32,48 +33,53 @@ PacBio reads generated using both CLR and CCS technology are filtered using `BLA ## Alignment and Mark duplicates +### Output options + +- **outfmt**: Specifies the output format for alignments. It can be set to "bam", "cram", or both, separated by a comma (e.g., `--outfmt bam,cram`). The pipeline will generate output files in the specified formats. +- **compression**: Specifies the compression method for alignments. It can be set to "none" or "crumble". When set to "crumble", the pipeline compresses the quality scores of the alignments. + ### Short reads -Short read data from HiC and Illumina technologies is aligned with `BWAMEM2_MEM`. The sorted and merged alignment files are processed using the `SAMTOOLS` [mark-duplicate workflow](https://www.htslib.org/algorithms/duplicate.html#workflow). The mark duplicate alignments is output in the CRAM format, along with the index. +Short read data from HiC and Illumina technologies is aligned with `BWAMEM2_MEM`. The sorted and merged alignment files are processed using the `SAMTOOLS` [mark-duplicate workflow](https://www.htslib.org/algorithms/duplicate.html#workflow). The marked duplicate alignments are output in the CRAM or BAM format, along with the index.
    Output files - `read_mapping` - `hic` - - `.unmasked.hic..cram`: Sorted and merged CRAM file at the individual level - - `.unmasked.hic..cram.crai`: Index for the alignment + - `.unmasked.hic..[cr|b]am`: Sorted and merged BAM or CRAM file at the individual level + - `.unmasked.hic..[cr|b]am.[cr|c]si`: Index for the alignment (as either .csi or .crai) - `illumina` - - `.unmasked.illumina..cram`: Sorted and merged CRAM file at the individual level - - `.unmasked.illumina..cram.crai`: Index for the alignment + - `.unmasked.illumina..[cr|b]am`: Sorted and merged BAM or CRAM file at the individual level + - `.unmasked.illumina..[cr|b]am.[cr|c]si`: Index for the alignment
    ### Oxford Nanopore reads -Reads generated using Oxford Nanopore technology are aligned with `MINIMAP2_ALIGN`. The sorted and merged alignment is output in the CRAM format, along with the index. +Reads generated using Oxford Nanopore technology are aligned with `MINIMAP2_ALIGN`. The sorted and merged alignment is output in the CRAM or BAM format, along with the index.
    Output files - `read_mapping` - `ont` - - `.unmasked.ont..cram`: Sorted and merged CRAM file at the individual level - - `.unmasked.ont..cram.crai`: Index for the alignment + - `.unmasked.ont..[cr|b]am`: Sorted and merged BAM or CRAM file at the individual level + - `.unmasked.ont..[cr|b]am.[cr|c]si`: Index for the alignment
    ### PacBio reads -The filtered PacBio reads are aligned with `MINIMAP2_ALIGN`. The sorted and merged alignment is output in the CRAM format, along with the index. +The filtered PacBio reads are aligned with `MINIMAP2_ALIGN`. The sorted and merged alignment is output in the CRAM or BAM format, along with the index.
    Output files - `read_mapping` - `pacbio` - - `.unmasked.pacbio..cram`: Sorted and merged CRAM file at the individual level - - `.unmasked.pacbio..cram.crai`: Index for the alignment + - `.unmasked.pacbio..[cr|b]am`: Sorted and merged BAM or CRAM file at the individual level + - `.unmasked.pacbio..[cr|b]am.[cr|c]si`: Index for the alignment
    @@ -81,7 +87,7 @@ The filtered PacBio reads are aligned with `MINIMAP2_ALIGN`. The sorted and merg ### External metadata -If provided using the `--header` option, all output alignments (`*.cram`) will include any additional metadata supplied as a SAM header template, replacing the existing _@HD_ and _@SD_ entries (note that this behaviour can be altered by modifying the `ext.args` for `SAMTOOLS_REHEADER` in `modules.config`. +If provided using the `--header` option, all output alignments (`*.cram` or `*.bam`) will include any additional metadata supplied as a SAM header template, replacing the existing _@HD_ and _@SD_ entries (note that this behaviour can be altered by modifying the `ext.args` for `SAMTOOLS_REHEADER` in `modules.config`). ### Statistics diff --git a/docs/usage.md b/docs/usage.md index 9a02085..9423325 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -42,7 +42,7 @@ sample1_T5,pacbio,pacbio2.bam,pacbio2 | ---------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (\_). | | `datatype` | Type of sequencing data. Must be one of `hic`, `Illumina`, `pacbio`, or `ont`. | -| `datafile` | Full path to read data file. Must be `bam` or `cram` or `fastq.gz` or `fq.gz` for `Illumina` and `HiC`. Must be `bam` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | +| `datafile` | Full path to read data file. Must be `bam`, `cram`, `fastq.gz` or `fq.gz` for `Illumina` and `HiC`. Must be `bam`, `fastq.gz` or `fq.gz` for `pacbio`. Must be `fastq.gz` or `fq.gz` for `ont`. | | `library` | (Optional) The library value is a unique identifier which is assigned to read group (`@RG`) ID. If the library name is not specified, the pipeline will auto-create library name using the data filename provided in the samplesheet. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. diff --git a/nextflow.config b/nextflow.config index bdad598..85e8051 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,6 +16,10 @@ params { fasta = null header = null + // Output format options + outfmt = "cram" + compression = "crumble" + // Execution options use_work_dir_as_temp = false diff --git a/nextflow_schema.json b/nextflow_schema.json index a3178b3..2209d6b 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,7 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "outdir"], + "required": ["input", "outdir", "outfmt", "compression"], "properties": { "input": { "type": "string", @@ -29,6 +29,18 @@ "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", "fa_icon": "fas fa-folder-open" }, + "outfmt": { + "type": "string", + "default": "cram", + "description": "Format for output alignment files. Accepts options: \"bam\" or \"cram\".", + "fa_icon": "fas fa-file-signature" + }, + "compression": { + "type": "string", + "default": "crumble", + "description": "Output file compression type. Currently accepts: \"none\" or \"crumble\"", + "fa_icon": "fas fa-file-archive" + }, "vector_db": { "type": "string", "description": "Path to directory or tar.gz archive for pre-built PacBio vector database.", diff --git a/subworkflows/local/convert_stats.nf b/subworkflows/local/convert_stats.nf index 66308e0..76e3f6d 100644 --- a/subworkflows/local/convert_stats.nf +++ b/subworkflows/local/convert_stats.nf @@ -2,70 +2,115 @@ // Convert BAM to CRAM, create index and calculate statistics // -include { CRUMBLE } from '../../modules/nf-core/crumble/main' -include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main' -include { SAMTOOLS_STATS } from '../../modules/nf-core/samtools/stats/main' -include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/samtools/flagstat/main' -include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/samtools/idxstats/main' +include { CRUMBLE } from '../../modules/nf-core/crumble/main' +include { SAMTOOLS_VIEW as SAMTOOLS_CRAM } from '../../modules/nf-core/samtools/view/main' +include { SAMTOOLS_VIEW as SAMTOOLS_REINDEX } from '../../modules/nf-core/samtools/view/main' +include { SAMTOOLS_STATS } from '../../modules/nf-core/samtools/stats/main' +include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/samtools/flagstat/main' +include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/samtools/idxstats/main' workflow CONVERT_STATS { take: - bam // channel: [ val(meta), /path/to/bam, /path/to/bai] + bam // channel: [ val(meta), /path/to/bam, /path/to/bai ] fasta // channel: [ val(meta), /path/to/fasta ] - main: ch_versions = Channel.empty() - // Compress the quality scores of Illumina and PacBio CCS alignments - bam - | branch { - meta, bam -> - run_crumble : meta.datatype == "hic" || meta.datatype == "illumina" || meta.datatype == "pacbio" - no_crumble: true + + // Split outfmt parameter into a list + def outfmt_options = params.outfmt.split(',').collect { it.trim() } + + + // (Optionally) Compress the quality scores of Illumina and PacBio CCS alignments + if ( params.compression == "crumble" ) { + bam + | branch { + meta, bam -> + run_crumble: meta.datatype == "hic" || meta.datatype == "illumina" || meta.datatype == "pacbio" + no_crumble: true + } + | set { ch_bams } + + CRUMBLE ( ch_bams.run_crumble, [], [] ) + ch_versions = ch_versions.mix( CRUMBLE.out.versions ) + + // Convert BAM to CRAM + CRUMBLE.out.bam + | mix( ch_bams.no_crumble ) + | map { meta, bam -> [meta, bam, []] } + | set { ch_bams_for_conversion } + + } else { + bam + | map { meta, bam -> [meta, bam, []] } + | set { ch_bams_for_conversion } + } + + + // (Optionally) convert to CRAM if it's specified in outfmt + ch_cram = Channel.empty() + ch_crai = Channel.empty() + + if ("cram" in outfmt_options) { + SAMTOOLS_CRAM ( ch_bams_for_conversion, fasta, [] ) + ch_versions = ch_versions.mix( SAMTOOLS_CRAM.out.versions.first() ) + + // Combine CRAM and CRAI into one channel + ch_cram = SAMTOOLS_CRAM.out.cram + ch_crai = SAMTOOLS_CRAM.out.crai } - | set { ch_bams } - CRUMBLE ( ch_bams.run_crumble, [], [] ) - ch_versions = ch_versions.mix ( CRUMBLE.out.versions ) + // Re-generate BAM index if BAM is in outfmt + def ch_data_for_stats + if ("cram" in outfmt_options) { + ch_data_for_stats = ch_cram.join( ch_crai ) + } else { + ch_data_for_stats = ch_bams_for_conversion + } + + ch_bam = Channel.empty() + ch_bai = Channel.empty() - // Convert BAM to CRAM - CRUMBLE.out.bam - | mix ( ch_bams.no_crumble ) - | map { meta, bam -> [meta, bam, []] } - | set { ch_bams_for_conversion } + if ("bam" in outfmt_options) { + // Re-generate BAM index + SAMTOOLS_REINDEX ( ch_bams_for_conversion, fasta, [] ) + ch_versions = ch_versions.mix( SAMTOOLS_REINDEX.out.versions.first() ) - SAMTOOLS_VIEW ( ch_bams_for_conversion, fasta, [] ) - ch_versions = ch_versions.mix ( SAMTOOLS_VIEW.out.versions.first() ) + // Set the BAM and BAI channels for emission + ch_bam = SAMTOOLS_REINDEX.out.bam + ch_bai = SAMTOOLS_REINDEX.out.bai - // Combine CRAM and CRAI into one channel - SAMTOOLS_VIEW.out.cram - | join ( SAMTOOLS_VIEW.out.crai ) - | set { ch_cram_crai } + // If using BAM for stats, use the reindexed BAM + if ( !("cram" in outfmt_options) ) { + ch_data_for_stats = ch_bam.join ( ch_bai ) + } + } // Calculate statistics - SAMTOOLS_STATS ( ch_cram_crai, fasta ) - ch_versions = ch_versions.mix ( SAMTOOLS_STATS.out.versions.first() ) + SAMTOOLS_STATS ( ch_data_for_stats, fasta ) + ch_versions = ch_versions.mix( SAMTOOLS_STATS.out.versions.first() ) // Calculate statistics based on flag values - SAMTOOLS_FLAGSTAT ( ch_cram_crai ) - ch_versions = ch_versions.mix ( SAMTOOLS_FLAGSTAT.out.versions.first() ) + SAMTOOLS_FLAGSTAT ( ch_data_for_stats ) + ch_versions = ch_versions.mix( SAMTOOLS_FLAGSTAT.out.versions.first() ) // Calculate index statistics - SAMTOOLS_IDXSTATS ( ch_cram_crai ) - ch_versions = ch_versions.mix ( SAMTOOLS_IDXSTATS.out.versions.first() ) - + SAMTOOLS_IDXSTATS ( ch_data_for_stats ) + ch_versions = ch_versions.mix( SAMTOOLS_IDXSTATS.out.versions.first() ) emit: - cram = SAMTOOLS_VIEW.out.cram // channel: [ val(meta), /path/to/cram ] - crai = SAMTOOLS_VIEW.out.crai // channel: [ val(meta), /path/to/crai ] - stats = SAMTOOLS_STATS.out.stats // channel: [ val(meta), /path/to/stats ] - flagstat = SAMTOOLS_FLAGSTAT.out.flagstat // channel: [ val(meta), /path/to/idxstats ] - idxstats = SAMTOOLS_IDXSTATS.out.idxstats // channel: [ val(meta), /path/to/flagstat ] - versions = ch_versions // channel: [ versions.yml ] + bam = ch_bam // channel: [ val(meta), /path/to/bam ] (optional) + bai = ch_bai // channel: [ val(meta), /path/to/bai ] (optional) + cram = ch_cram // channel: [ val(meta), /path/to/cram ] (optional) + crai = ch_crai // channel: [ val(meta), /path/to/crai ] (optional) + stats = SAMTOOLS_STATS.out.stats // channel: [ val(meta), /path/to/stats ] + flagstat = SAMTOOLS_FLAGSTAT.out.flagstat // channel: [ val(meta), /path/to/flagstat ] + idxstats = SAMTOOLS_IDXSTATS.out.idxstats // channel: [ val(meta), /path/to/idxstats ] + versions = ch_versions // channel: [ versions.yml ] } diff --git a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf index abfed43..92485e0 100644 --- a/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_readmapping_pipeline/main.nf @@ -168,11 +168,29 @@ workflow PIPELINE_COMPLETION { // Check and validate pipeline parameters // def validateInputParameters() { - + // Validate fasta parameter if (!params.fasta) { log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." } + // Validate outfmt parameter + if (!params.outfmt) { + log.error "Output format not specified. Please specify '--outfmt bam', '--outfmt cram', or both separated by a comma." + } else { + def outfmtOptions = params.outfmt.split(',').collect { it.trim() } + def validOutfmtOptions = ['bam', 'cram'] + def invalidOptions = outfmtOptions.findAll { !(it in validOutfmtOptions) } + + if (invalidOptions) { + log.error "Invalid output format(s) specified: '${invalidOptions.join(', ')}'. Valid options are 'bam' or 'cram'." + } + } + // Validate compression parameter + if (!params.compression) { + log.error "Compression option not specified. Please specify '--compression none' or '--compression crumble'." + } else if (!(params.compression in ['none', 'crumble'])) { + log.error "Invalid compression option specified: '${params.compression}'. Valid options are 'none' or 'crumble'." + } } // From e1492be17db2bbffa4ec492cfefca6eaf235a0d3 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 19 Aug 2024 14:56:35 +0100 Subject: [PATCH 098/111] Update CHANGELOG.md --- CHANGELOG.md | 5 ++++- 1 file changed, 4 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index d7944ac..838f3d5 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,12 +3,15 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## [[1.3.0](https://github.com/sanger-tol/readmapping/releases/tag/1.3.0)] - Antipodean Opaleye - [2024-06-XX] +## [[1.3.0](https://github.com/sanger-tol/readmapping/releases/tag/1.3.0)] - Antipodean Opaleye - [2024-08-19] ### Enhancements & fixes - Combined steps to improve the efficiency of the pipeline, especially on large genomes - "crumble" is now run on _every_ data type, not just PacBio +- Added options for output format and to turn on/off crumble compression (https://github.com/sanger-tol/readmapping/pull/107) +- Added `fastq` as possible input type for PacBio (https://github.com/sanger-tol/readmapping/pull/106) and Illumina/HiC (https://github.com/sanger-tol/readmapping/pull/96) +- Disabled running `bwa index` when no short-read data provided (https://github.com/sanger-tol/readmapping/pull/100) ### Software dependencies From 5e4ad2a4b7efb874daace92cbfcb191e3bc45eeb Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Mon, 19 Aug 2024 14:59:39 +0100 Subject: [PATCH 099/111] Update README.md --- README.md | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/README.md b/README.md index c289d56..00841b8 100644 --- a/README.md +++ b/README.md @@ -53,7 +53,9 @@ We thank the following people for their extensive assistance in the development - [Matthieu Muffato](https://github.com/muffato) for the text logo - [Guoying Qi](https://github.com/gq1) for being able to run tests using Nf-Tower and the Sanger HPC farm -- [Tyler Chafin](https://github.com/tkchafin) for maintenance and updates +- [Tyler Chafin](https://github.com/tkchafin) for maintenance, updates, and code reviews +- [Chau Duong](https://github.com/reichan1998) for updates and code reviews +- [Sandra Babirye](https://github.com/SandraBabirye) for updates and code reviews ## Contributions and Support From 0560e2860c76ad10c8176631890c205b790845c8 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Tue, 20 Aug 2024 09:15:43 +0100 Subject: [PATCH 100/111] Update CHANGELOG.md --- CHANGELOG.md | 15 ++++++++++++++- 1 file changed, 14 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 838f3d5..339afc1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -11,7 +11,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - "crumble" is now run on _every_ data type, not just PacBio - Added options for output format and to turn on/off crumble compression (https://github.com/sanger-tol/readmapping/pull/107) - Added `fastq` as possible input type for PacBio (https://github.com/sanger-tol/readmapping/pull/106) and Illumina/HiC (https://github.com/sanger-tol/readmapping/pull/96) -- Disabled running `bwa index` when no short-read data provided (https://github.com/sanger-tol/readmapping/pull/100) +- Disabled running `bwa index` when no short-read data provided (https://github.com/sanger-tol/readmapping/pull/100) +- Added support for optional custom SAM header (https://github.com/sanger-tol/readmapping/pull/95) +- Switch to `nf-validation` (https://github.com/sanger-tol/readmapping/pull/99) and further updates for nf-core v2.14 compliance (https://github.com/sanger-tol/readmapping/pull/98) ### Software dependencies @@ -19,10 +21,21 @@ Note, since the pipeline is using Nextflow DSL2, each process will be run with i | Dependency | Old version | New version | | ---------- | ------------- | ------------- | +| `blastn` | 2.13 | 2.15 | | `samtools` | 1.14 and 1.17 | 1.17 and 1.18 | > **NB:** Dependency has been **updated** if both old and new version information is present.
    **NB:** Dependency has been **added** if just the new version information is present.
    **NB:** Dependency has been **removed** if version information isn't present. +### Parameters + +| Old parameter | New parameter | +| ------------------ | ------------- | +| `--enable_conda` | | +| `--minimap2_index` | | +| `--samtools_index` | | + +> **NB:** Parameter has been **updated** if both old and new parameter information is present.
    **NB:** Parameter has been **added** if just the new parameter information is present.
    **NB:** Parameter has been **removed** if new parameter information isn't present. + ## [[1.2.2](https://github.com/sanger-tol/readmapping/releases/tag/1.2.2)] - Norwegian Ridgeback (patch 2) - [2024-05-23] ### Enhancements & fixes From 1a0d590445fdcd3669aa8965aae685664a2f33bc Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Tue, 20 Aug 2024 09:22:55 +0100 Subject: [PATCH 101/111] module updates for 1.3.0 --- modules.json | 22 +- .../nf-core/blast/blastn/tests/main.nf.test | 6 +- .../nf-core/bwamem2/mem/tests/main.nf.test | 99 ++---- .../bwamem2/mem/tests/main.nf.test.snap | 108 +++++-- modules/nf-core/fastqc/environment.yml | 7 - modules/nf-core/fastqc/main.nf | 61 ---- modules/nf-core/fastqc/meta.yml | 57 ---- modules/nf-core/fastqc/tests/main.nf.test | 212 ------------- .../nf-core/fastqc/tests/main.nf.test.snap | 88 ------ modules/nf-core/fastqc/tests/tags.yml | 2 - modules/nf-core/minimap2/align/main.nf | 15 +- .../nf-core/minimap2/align/tests/main.nf.test | 205 ++++++++++++- .../minimap2/align/tests/main.nf.test.snap | 284 +++++++++++++++++- modules/nf-core/multiqc/environment.yml | 7 - modules/nf-core/multiqc/main.nf | 55 ---- modules/nf-core/multiqc/meta.yml | 58 ---- modules/nf-core/multiqc/tests/main.nf.test | 84 ------ .../nf-core/multiqc/tests/main.nf.test.snap | 41 --- modules/nf-core/multiqc/tests/tags.yml | 2 - .../samtools/flagstat/tests/main.nf.test | 28 +- .../samtools/flagstat/tests/main.nf.test.snap | 78 +++-- .../samtools/idxstats/tests/main.nf.test | 29 +- .../samtools/idxstats/tests/main.nf.test.snap | 78 +++-- .../nf-core/samtools/stats/tests/main.nf.test | 60 +++- .../samtools/stats/tests/main.nf.test.snap | 82 ++++- 25 files changed, 901 insertions(+), 867 deletions(-) delete mode 100644 modules/nf-core/fastqc/environment.yml delete mode 100644 modules/nf-core/fastqc/main.nf delete mode 100644 modules/nf-core/fastqc/meta.yml delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap delete mode 100644 modules/nf-core/fastqc/tests/tags.yml delete mode 100644 modules/nf-core/multiqc/environment.yml delete mode 100644 modules/nf-core/multiqc/main.nf delete mode 100644 modules/nf-core/multiqc/meta.yml delete mode 100644 modules/nf-core/multiqc/tests/main.nf.test delete mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap delete mode 100644 modules/nf-core/multiqc/tests/tags.yml diff --git a/modules.json b/modules.json index 0653c8f..bcabde0 100644 --- a/modules.json +++ b/modules.json @@ -7,7 +7,7 @@ "nf-core": { "blast/blastn": { "branch": "master", - "git_sha": "4262a04142431275e54e1f4b413628a2201ed6e6", + "git_sha": "583edaf97c9373a20df05a3b7be5a6677f9cd719", "installed_by": ["modules"] }, "bwamem2/index": { @@ -17,7 +17,7 @@ }, "bwamem2/mem": { "branch": "master", - "git_sha": "3afb95b2e15fc4a2347470255a7ef654f650c8ec", + "git_sha": "2201e21b09213f083832ac58e33353d410a6fde7", "installed_by": ["modules"] }, "crumble": { @@ -30,11 +30,6 @@ "git_sha": "82024cf6325d2ee194e7f056d841ecad2f6856e9", "installed_by": ["modules"] }, - "fastqc": { - "branch": "master", - "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", - "installed_by": ["modules"] - }, "gunzip": { "branch": "master", "git_sha": "4e5f4687318f24ba944a13609d3ea6ebd890737d", @@ -42,12 +37,7 @@ }, "minimap2/align": { "branch": "master", - "git_sha": "e83b347b3e674de6bb1bb7bdf9b2674768c8e0fe", - "installed_by": ["modules"] - }, - "multiqc": { - "branch": "master", - "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", + "git_sha": "a33ef9475558c6b8da08c5f522ddaca1ec810306", "installed_by": ["modules"] }, "samtools/faidx": { @@ -62,12 +52,12 @@ }, "samtools/flagstat": { "branch": "master", - "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", + "git_sha": "46eca555142d6e597729fcb682adcc791796f514", "installed_by": ["modules"] }, "samtools/idxstats": { "branch": "master", - "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", + "git_sha": "46eca555142d6e597729fcb682adcc791796f514", "installed_by": ["modules"] }, "samtools/merge": { @@ -78,7 +68,7 @@ }, "samtools/stats": { "branch": "master", - "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", + "git_sha": "1fe379cf6e6c1e6fa5e32bcbeefea0f1e874dac6", "installed_by": ["modules"] }, "samtools/view": { diff --git a/modules/nf-core/blast/blastn/tests/main.nf.test b/modules/nf-core/blast/blastn/tests/main.nf.test index 02ecfab..aacc93c 100644 --- a/modules/nf-core/blast/blastn/tests/main.nf.test +++ b/modules/nf-core/blast/blastn/tests/main.nf.test @@ -15,7 +15,7 @@ nextflow_process { script "../../makeblastdb/main.nf" process { """ - input[0] = [ [id:'test2'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + input[0] = [ [id:'test2'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } } @@ -29,7 +29,7 @@ nextflow_process { } process { """ - input[0] = [ [id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + input[0] = [ [id:'test'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] input[1] = BLAST_MAKEBLASTDB.out.db """ } @@ -53,7 +53,7 @@ nextflow_process { } process { """ - input[0] = [ [id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta_gz'], checkIfExists: true) ] + input[0] = [ [id:'test'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) ] input[1] = BLAST_MAKEBLASTDB.out.db """ } diff --git a/modules/nf-core/bwamem2/mem/tests/main.nf.test b/modules/nf-core/bwamem2/mem/tests/main.nf.test index 5e67f70..9e0ab14 100644 --- a/modules/nf-core/bwamem2/mem/tests/main.nf.test +++ b/modules/nf-core/bwamem2/mem/tests/main.nf.test @@ -10,21 +10,21 @@ nextflow_process { tag "bwamem2/mem" tag "bwamem2/index" - test("sarscov2 - fastq, index, fasta, false") { - - setup { - run("BWAMEM2_INDEX") { - script "../../index/main.nf" - process { - """ - input[0] = Channel.of([ - [:], // meta map - [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] - ]) - """ - } + setup { + run("BWAMEM2_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) + """ } } + } + + test("sarscov2 - fastq, index, fasta, false") { when { process { @@ -44,7 +44,8 @@ nextflow_process { assertAll( { assert process.success }, { assert snapshot( - file(process.out.bam[0][1]).name, + bam(process.out.bam[0][1]).getHeaderMD5(), + bam(process.out.bam[0][1]).getReadsMD5(), process.out.versions ).match() } ) @@ -54,20 +55,6 @@ nextflow_process { test("sarscov2 - fastq, index, fasta, true") { - setup { - run("BWAMEM2_INDEX") { - script "../../index/main.nf" - process { - """ - input[0] = Channel.of([ - [:], // meta map - [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] - ]) - """ - } - } - } - when { process { """ @@ -86,7 +73,8 @@ nextflow_process { assertAll( { assert process.success }, { assert snapshot( - file(process.out.bam[0][1]).name, + bam(process.out.bam[0][1]).getHeaderMD5(), + bam(process.out.bam[0][1]).getReadsMD5(), process.out.versions ).match() } ) @@ -96,20 +84,6 @@ nextflow_process { test("sarscov2 - [fastq1, fastq2], index, fasta, false") { - setup { - run("BWAMEM2_INDEX") { - script "../../index/main.nf" - process { - """ - input[0] = Channel.of([ - [:], // meta map - [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] - ]) - """ - } - } - } - when { process { """ @@ -131,7 +105,8 @@ nextflow_process { assertAll( { assert process.success }, { assert snapshot( - file(process.out.bam[0][1]).name, + bam(process.out.bam[0][1]).getHeaderMD5(), + bam(process.out.bam[0][1]).getReadsMD5(), process.out.versions ).match() } ) @@ -141,20 +116,6 @@ nextflow_process { test("sarscov2 - [fastq1, fastq2], index, fasta, true") { - setup { - run("BWAMEM2_INDEX") { - script "../../index/main.nf" - process { - """ - input[0] = Channel.of([ - [:], // meta map - [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] - ]) - """ - } - } - } - when { process { """ @@ -176,7 +137,8 @@ nextflow_process { assertAll( { assert process.success }, { assert snapshot( - file(process.out.bam[0][1]).name, + bam(process.out.bam[0][1]).getHeaderMD5(), + bam(process.out.bam[0][1]).getReadsMD5(), process.out.versions ).match() } ) @@ -188,20 +150,6 @@ nextflow_process { options "-stub" - setup { - run("BWAMEM2_INDEX") { - script "../../index/main.nf" - process { - """ - input[0] = Channel.of([ - [:], // meta map - [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] - ]) - """ - } - } - } - when { process { """ @@ -222,10 +170,7 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - process.out.versions - ).match() } + { assert snapshot(process.out).match() } ) } diff --git a/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap b/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap index 9fb1e69..69bc361 100644 --- a/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap +++ b/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap @@ -1,67 +1,129 @@ { "sarscov2 - [fastq1, fastq2], index, fasta, false": { "content": [ - "test.bam", + "eefa0f44493fd0504e734efd2f1f4a9e", + "57aeef88ed701a8ebc8e2f0a381b2a6", [ "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-03-19T13:13:18.890289958" + "timestamp": "2024-08-02T12:23:37.929675" }, "sarscov2 - [fastq1, fastq2], index, fasta, true - stub": { "content": [ - "test.bam", - [ - "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" - ] + { + "0": [ + + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + + ], + "3": [ + + ], + "4": [ + [ + { + "id": "test", + "single_end": false + }, + "test.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "5": [ + "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" + ], + "bam": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "crai": [ + + ], + "cram": [ + + ], + "csi": [ + [ + { + "id": "test", + "single_end": false + }, + "test.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "sam": [ + + ], + "versions": [ + "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" + ] + } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-03-19T13:45:51.821633029" + "timestamp": "2024-08-02T12:12:06.693567" }, "sarscov2 - [fastq1, fastq2], index, fasta, true": { "content": [ - "test.bam", + "7aba324f82d5b4e926a5dd7b46029cb4", + "af8628d9df18b2d3d4f6fd47ef2bb872", [ "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-03-19T13:13:36.458291078" + "timestamp": "2024-08-02T12:23:53.488374" }, "sarscov2 - fastq, index, fasta, false": { "content": [ - "test.bam", + "bc02b41697b3a8f1021b02becec24052", + "798439cbd7fd81cbcc5078022dc5479d", [ "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-03-19T13:12:44.084654507" + "timestamp": "2024-08-02T12:23:05.644682" }, "sarscov2 - fastq, index, fasta, true": { "content": [ - "test.bam", + "e41d67320815d29ba5f6e9d1ae21902a", + "94fcf617f5b994584c4e8d4044e16b4f", [ "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-03-19T13:13:01.763341681" + "timestamp": "2024-08-02T12:23:21.837763" } } \ No newline at end of file diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml deleted file mode 100644 index 1787b38..0000000 --- a/modules/nf-core/fastqc/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: fastqc -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf deleted file mode 100644 index d79f1c8..0000000 --- a/modules/nf-core/fastqc/main.nf +++ /dev/null @@ -1,61 +0,0 @@ -process FASTQC { - tag "$meta.id" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : - 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" - - input: - tuple val(meta), path(reads) - - output: - tuple val(meta), path("*.html"), emit: html - tuple val(meta), path("*.zip") , emit: zip - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - // Make list of old name and new name pairs to use for renaming in the bash while loop - def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } - def rename_to = old_new_pairs*.join(' ').join(' ') - def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') - - def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') - // FastQC memory value allowed range (100 - 10000) - def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb) - - """ - printf "%s %s\\n" $rename_to | while read old_name new_name; do - [ -f "\${new_name}" ] || ln -s \$old_name \$new_name - done - - fastqc \\ - $args \\ - --threads $task.cpus \\ - --memory $fastqc_memory \\ - $renamed_files - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.html - touch ${prefix}.zip - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml deleted file mode 100644 index ee5507e..0000000 --- a/modules/nf-core/fastqc/meta.yml +++ /dev/null @@ -1,57 +0,0 @@ -name: fastqc -description: Run FastQC on sequenced reads -keywords: - - quality control - - qc - - adapters - - fastq -tools: - - fastqc: - description: | - FastQC gives general quality metrics about your reads. - It provides information about the quality score distribution - across your reads, the per base sequence content (%A/C/G/T). - You get information about adapter contamination and other - overrepresented sequences. - homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ - documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ - licence: ["GPL-2.0-only"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - html: - type: file - description: FastQC report - pattern: "*_{fastqc.html}" - - zip: - type: file - description: FastQC report archive - pattern: "*_{fastqc.zip}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" -maintainers: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test deleted file mode 100644 index 70edae4..0000000 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ /dev/null @@ -1,212 +0,0 @@ -nextflow_process { - - name "Test Process FASTQC" - script "../main.nf" - process "FASTQC" - - tag "modules" - tag "modules_nfcore" - tag "fastqc" - - test("sarscov2 single-end [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [ id: 'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. - // looks like this:
    Mon 2 Oct 2023
    test.gz
    - // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_single") } - ) - } - } - - test("sarscov2 paired-end [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_paired") } - ) - } - } - - test("sarscov2 interleaved [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } - ) - } - } - - test("sarscov2 paired-end [bam]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_bam") } - ) - } - } - - test("sarscov2 multiple [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, - { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, - { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][2]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][3]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } - ) - } - } - - test("sarscov2 custom_prefix") { - - when { - process { - """ - input[0] = Channel.of([ - [ id:'mysample', single_end:true ], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } - ) - } - } - - test("sarscov2 single-end [fastq] - stub") { - - options "-stub" - - when { - process { - """ - input[0] = Channel.of([ - [ id: 'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert snapshot(process.out.html.collect { file(it[1]).getName() } + - process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match("fastqc_stub") } - ) - } - } - -} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap deleted file mode 100644 index 86f7c31..0000000 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ /dev/null @@ -1,88 +0,0 @@ -{ - "fastqc_versions_interleaved": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:40:07.293713" - }, - "fastqc_stub": { - "content": [ - [ - "test.html", - "test.zip", - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:31:01.425198" - }, - "fastqc_versions_multiple": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:40:55.797907" - }, - "fastqc_versions_bam": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:40:26.795862" - }, - "fastqc_versions_single": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:39:27.043675" - }, - "fastqc_versions_paired": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:39:47.584191" - }, - "fastqc_versions_custom_prefix": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:41:14.576531" - } -} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml deleted file mode 100644 index 7834294..0000000 --- a/modules/nf-core/fastqc/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -fastqc: - - modules/nf-core/fastqc/** diff --git a/modules/nf-core/minimap2/align/main.nf b/modules/nf-core/minimap2/align/main.nf index 66745ef..d82dc14 100644 --- a/modules/nf-core/minimap2/align/main.nf +++ b/modules/nf-core/minimap2/align/main.nf @@ -28,17 +28,25 @@ process MINIMAP2_ALIGN { script: def args = task.ext.args ?: '' def args2 = task.ext.args2 ?: '' + def args3 = task.ext.args3 ?: '' + def args4 = task.ext.args4 ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def bam_index = bam_index_extension ? "${prefix}.bam##idx##${prefix}.bam.${bam_index_extension} --write-index" : "${prefix}.bam" def bam_output = bam_format ? "-a | samtools sort -@ ${task.cpus-1} -o ${bam_index} ${args2}" : "-o ${prefix}.paf" def cigar_paf = cigar_paf_format && !bam_format ? "-c" : '' def set_cigar_bam = cigar_bam && bam_format ? "-L" : '' + def bam_input = "${reads.extension}".matches('sam|bam|cram') + def samtools_reset_fastq = bam_input ? "samtools reset --threads ${task.cpus-1} $args3 $reads | samtools fastq --threads ${task.cpus-1} $args4 |" : '' + def query = bam_input ? "-" : reads + def target = reference ?: (bam_input ? error("BAM input requires reference") : reads) + """ + $samtools_reset_fastq \\ minimap2 \\ $args \\ -t $task.cpus \\ - ${reference ?: reads} \\ - $reads \\ + $target \\ + $query \\ $cigar_paf \\ $set_cigar_bam \\ $bam_output @@ -55,6 +63,9 @@ process MINIMAP2_ALIGN { def prefix = task.ext.prefix ?: "${meta.id}" def output_file = bam_format ? "${prefix}.bam" : "${prefix}.paf" def bam_index = bam_index_extension ? "touch ${prefix}.bam.${bam_index_extension}" : "" + def bam_input = "${reads.extension}".matches('sam|bam|cram') + def target = reference ?: (bam_input ? error("BAM input requires reference") : reads) + """ touch $output_file ${bam_index} diff --git a/modules/nf-core/minimap2/align/tests/main.nf.test b/modules/nf-core/minimap2/align/tests/main.nf.test index c93f436..4072c17 100644 --- a/modules/nf-core/minimap2/align/tests/main.nf.test +++ b/modules/nf-core/minimap2/align/tests/main.nf.test @@ -34,7 +34,8 @@ nextflow_process { assertAll( { assert process.success }, { assert snapshot( - file(process.out.bam[0][1]).name, + bam(process.out.bam[0][1]).getHeader(), + bam(process.out.bam[0][1]).getReadsMD5(), process.out.versions ).match() } ) @@ -67,7 +68,8 @@ nextflow_process { assertAll( { assert process.success }, { assert snapshot( - file(process.out.bam[0][1]).name, + bam(process.out.bam[0][1]).getHeader(), + bam(process.out.bam[0][1]).getReadsMD5(), file(process.out.index[0][1]).name, process.out.versions ).match() } @@ -104,7 +106,8 @@ nextflow_process { assertAll( { assert process.success }, { assert snapshot( - file(process.out.bam[0][1]).name, + bam(process.out.bam[0][1]).getHeader(), + bam(process.out.bam[0][1]).getReadsMD5(), process.out.versions ).match() } ) @@ -137,7 +140,8 @@ nextflow_process { assertAll( { assert process.success }, { assert snapshot( - file(process.out.bam[0][1]).name, + bam(process.out.bam[0][1]).getHeader(), + bam(process.out.bam[0][1]).getReadsMD5(), process.out.versions ).match() } ) @@ -145,6 +149,104 @@ nextflow_process { } + test("sarscov2 - bam, fasta, true, [], false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + bam(process.out.bam[0][1]).getHeader(), + bam(process.out.bam[0][1]).getReadsMD5(), + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - bam, fasta, true, 'bai', false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = 'bai' + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + bam(process.out.bam[0][1]).getHeader(), + bam(process.out.bam[0][1]).getReadsMD5(), + file(process.out.index[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - bam, [], true, false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + [] + ] + input[2] = true + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.failed } + ) + } + + } + test("sarscov2 - fastq, fasta, true, [], false, false - stub") { options "-stub" @@ -241,4 +343,99 @@ nextflow_process { } + test("sarscov2 - bam, fasta, true, [], false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - bam, fasta, true, 'bai', false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = 'bai' + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - bam, [], true, false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + [] + ] + input[2] = true + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.failed } + ) + } + + } + } \ No newline at end of file diff --git a/modules/nf-core/minimap2/align/tests/main.nf.test.snap b/modules/nf-core/minimap2/align/tests/main.nf.test.snap index ecb3e4e..12264a8 100644 --- a/modules/nf-core/minimap2/align/tests/main.nf.test.snap +++ b/modules/nf-core/minimap2/align/tests/main.nf.test.snap @@ -1,4 +1,83 @@ { + "sarscov2 - bam, fasta, true, 'bai', false, false": { + "content": [ + [ + "@HD\tVN:1.6\tSO:coordinate", + "@SQ\tSN:MT192765.1\tLN:29829", + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta -", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam##idx##test.bam.bai --write-index" + ], + "5d426b9a5f5b2c54f1d7f1e4c238ae94", + "test.bam.bai", + [ + "versions.yml:md5,3548eeba9066efbf8d78ea99f8d813fd" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-07-25T09:03:00.827260362" + }, + "sarscov2 - bam, fasta, true, 'bai', false, false - stub": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ], + "bam": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "index": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "paf": [ + + ], + "versions": [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-07-23T11:21:37.92353539" + }, "sarscov2 - fastq, fasta, true, 'bai', false, false - stub": { "content": [ { @@ -154,7 +233,13 @@ }, "sarscov2 - [fastq1, fastq2], fasta, true, false, false": { "content": [ - "test.bam", + [ + "@HD\tVN:1.6\tSO:coordinate", + "@SQ\tSN:MT192765.1\tLN:29829", + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta test_1.fastq.gz test_2.fastq.gz", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam" + ], + "1bc392244f228bf52cf0b5a8f6a654c9", [ "versions.yml:md5,3548eeba9066efbf8d78ea99f8d813fd" ] @@ -163,11 +248,17 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-03T12:21:31.582849048" + "timestamp": "2024-07-23T11:18:18.964586894" }, "sarscov2 - fastq, fasta, true, [], false, false": { "content": [ - "test.bam", + [ + "@HD\tVN:1.6\tSO:coordinate", + "@SQ\tSN:MT192765.1\tLN:29829", + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta test_1.fastq.gz", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam" + ], + "f194745c0ccfcb2a9c0aee094a08750", [ "versions.yml:md5,3548eeba9066efbf8d78ea99f8d813fd" ] @@ -176,11 +267,17 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-03T12:29:11.968586416" + "timestamp": "2024-07-23T11:17:48.667488325" }, "sarscov2 - fastq, fasta, true, 'bai', false, false": { "content": [ - "test.bam", + [ + "@HD\tVN:1.6\tSO:coordinate", + "@SQ\tSN:MT192765.1\tLN:29829", + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta test_1.fastq.gz", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam##idx##test.bam.bai --write-index" + ], + "f194745c0ccfcb2a9c0aee094a08750", "test.bam.bai", [ "versions.yml:md5,3548eeba9066efbf8d78ea99f8d813fd" @@ -190,11 +287,182 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-03T12:21:20.362186362" + "timestamp": "2024-07-23T11:18:02.517416733" + }, + "sarscov2 - bam, fasta, true, [], false, false": { + "content": [ + [ + "@HD\tVN:1.6\tSO:coordinate", + "@SQ\tSN:MT192765.1\tLN:29829", + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta -", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam" + ], + "5d426b9a5f5b2c54f1d7f1e4c238ae94", + [ + "versions.yml:md5,3548eeba9066efbf8d78ea99f8d813fd" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-07-25T09:02:49.64829488" + }, + "sarscov2 - bam, fasta, true, [], false, false - stub": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ], + "bam": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "index": [ + + ], + "paf": [ + + ], + "versions": [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-07-23T11:21:22.162291795" }, "sarscov2 - fastq, [], true, false, false": { "content": [ - "test.bam", + [ + "@HD\tVN:1.6\tSO:coordinate", + "@SQ\tSN:ERR5069949.2151832\tLN:150", + "@SQ\tSN:ERR5069949.576388\tLN:77", + "@SQ\tSN:ERR5069949.501486\tLN:146", + "@SQ\tSN:ERR5069949.1331889\tLN:132", + 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"@SQ\tSN:ERR5069949.1067032\tLN:150", + "@SQ\tSN:ERR5069949.3338256\tLN:151", + "@SQ\tSN:ERR5069949.1412839\tLN:147", + "@SQ\tSN:ERR5069949.1538968\tLN:150", + "@SQ\tSN:ERR5069949.147998\tLN:94", + "@SQ\tSN:ERR5069949.366975\tLN:106", + "@SQ\tSN:ERR5069949.1372331\tLN:151", + "@SQ\tSN:ERR5069949.1709367\tLN:129", + "@SQ\tSN:ERR5069949.2388984\tLN:150", + "@SQ\tSN:ERR5069949.1132353\tLN:150", + "@SQ\tSN:ERR5069949.1151736\tLN:151", + "@SQ\tSN:ERR5069949.479807\tLN:150", + "@SQ\tSN:ERR5069949.2176303\tLN:151", + "@SQ\tSN:ERR5069949.2772897\tLN:151", + "@SQ\tSN:ERR5069949.1020777\tLN:122", + "@SQ\tSN:ERR5069949.465452\tLN:151", + "@SQ\tSN:ERR5069949.1704586\tLN:149", + "@SQ\tSN:ERR5069949.1258508\tLN:151", + "@SQ\tSN:ERR5069949.986441\tLN:119", + "@SQ\tSN:ERR5069949.2674295\tLN:148", + "@SQ\tSN:ERR5069949.885966\tLN:79", + "@SQ\tSN:ERR5069949.2342766\tLN:151", + "@SQ\tSN:ERR5069949.3122970\tLN:127", + "@SQ\tSN:ERR5069949.3279513\tLN:72", + "@SQ\tSN:ERR5069949.309410\tLN:151", + "@SQ\tSN:ERR5069949.532979\tLN:149", + "@SQ\tSN:ERR5069949.2888794\tLN:151", + "@SQ\tSN:ERR5069949.2205229\tLN:150", + "@SQ\tSN:ERR5069949.786562\tLN:151", + "@SQ\tSN:ERR5069949.919671\tLN:151", + "@SQ\tSN:ERR5069949.1328186\tLN:151", + "@SQ\tSN:ERR5069949.870926\tLN:149", + "@SQ\tSN:ERR5069949.2257580\tLN:151", + "@SQ\tSN:ERR5069949.3249622\tLN:77", + "@SQ\tSN:ERR5069949.611123\tLN:125", + "@SQ\tSN:ERR5069949.651338\tLN:142", + "@SQ\tSN:ERR5069949.169513\tLN:92", + "@SQ\tSN:ERR5069949.155944\tLN:150", + "@SQ\tSN:ERR5069949.2033605\tLN:150", + "@SQ\tSN:ERR5069949.2730382\tLN:142", + "@SQ\tSN:ERR5069949.2125592\tLN:150", + "@SQ\tSN:ERR5069949.1062611\tLN:151", + "@SQ\tSN:ERR5069949.1778133\tLN:151", + "@SQ\tSN:ERR5069949.3057020\tLN:95", + "@SQ\tSN:ERR5069949.2972968\tLN:141", + "@SQ\tSN:ERR5069949.2734474\tLN:149", + "@SQ\tSN:ERR5069949.856527\tLN:151", + "@SQ\tSN:ERR5069949.2098070\tLN:151", + "@SQ\tSN:ERR5069949.1552198\tLN:150", + "@SQ\tSN:ERR5069949.2385514\tLN:150", + "@SQ\tSN:ERR5069949.2270078\tLN:151", + "@SQ\tSN:ERR5069949.114870\tLN:150", + "@SQ\tSN:ERR5069949.2668880\tLN:147", + "@SQ\tSN:ERR5069949.257821\tLN:139", + "@SQ\tSN:ERR5069949.2243023\tLN:150", + "@SQ\tSN:ERR5069949.2605155\tLN:146", + "@SQ\tSN:ERR5069949.1340552\tLN:151", + "@SQ\tSN:ERR5069949.1561137\tLN:150", + "@SQ\tSN:ERR5069949.2361683\tLN:149", + "@SQ\tSN:ERR5069949.2521353\tLN:150", + "@SQ\tSN:ERR5069949.1261808\tLN:149", + "@SQ\tSN:ERR5069949.2734873\tLN:98", + "@SQ\tSN:ERR5069949.3017828\tLN:107", + "@SQ\tSN:ERR5069949.573706\tLN:150", + "@SQ\tSN:ERR5069949.1980512\tLN:151", + "@SQ\tSN:ERR5069949.1014693\tLN:150", + "@SQ\tSN:ERR5069949.3184655\tLN:150", + "@SQ\tSN:ERR5069949.29668\tLN:89", + "@SQ\tSN:ERR5069949.3258358\tLN:151", + "@SQ\tSN:ERR5069949.1476386\tLN:151", + "@SQ\tSN:ERR5069949.2415814\tLN:150", + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a test_1.fastq.gz test_1.fastq.gz", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam" + ], + "16c1c651f8ec67383bcdee3c55aed94f", [ "versions.yml:md5,3548eeba9066efbf8d78ea99f8d813fd" ] @@ -203,6 +471,6 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-03T12:21:37.233709954" + "timestamp": "2024-07-23T11:18:34.246998277" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml deleted file mode 100644 index ca39fb6..0000000 --- a/modules/nf-core/multiqc/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: multiqc -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.21 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf deleted file mode 100644 index 47ac352..0000000 --- a/modules/nf-core/multiqc/main.nf +++ /dev/null @@ -1,55 +0,0 @@ -process MULTIQC { - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : - 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" - - input: - path multiqc_files, stageAs: "?/*" - path(multiqc_config) - path(extra_multiqc_config) - path(multiqc_logo) - - output: - path "*multiqc_report.html", emit: report - path "*_data" , emit: data - path "*_plots" , optional:true, emit: plots - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def config = multiqc_config ? "--config $multiqc_config" : '' - def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' - def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' - """ - multiqc \\ - --force \\ - $args \\ - $config \\ - $extra_config \\ - $logo \\ - . - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) - END_VERSIONS - """ - - stub: - """ - mkdir multiqc_data - touch multiqc_plots - touch multiqc_report.html - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml deleted file mode 100644 index 45a9bc3..0000000 --- a/modules/nf-core/multiqc/meta.yml +++ /dev/null @@ -1,58 +0,0 @@ -name: multiqc -description: Aggregate results from bioinformatics analyses across many samples into a single report -keywords: - - QC - - bioinformatics tools - - Beautiful stand-alone HTML report -tools: - - multiqc: - description: | - MultiQC searches a given directory for analysis logs and compiles a HTML report. - It's a general use tool, perfect for summarising the output from numerous bioinformatics tools. - homepage: https://multiqc.info/ - documentation: https://multiqc.info/docs/ - licence: ["GPL-3.0-or-later"] -input: - - multiqc_files: - type: file - description: | - List of reports / files recognised by MultiQC, for example the html and zip output of FastQC - - multiqc_config: - type: file - description: Optional config yml for MultiQC - pattern: "*.{yml,yaml}" - - extra_multiqc_config: - type: file - description: Second optional config yml for MultiQC. Will override common sections in multiqc_config. - pattern: "*.{yml,yaml}" - - multiqc_logo: - type: file - description: Optional logo file for MultiQC - pattern: "*.{png}" -output: - - report: - type: file - description: MultiQC report file - pattern: "multiqc_report.html" - - data: - type: directory - description: MultiQC data dir - pattern: "multiqc_data" - - plots: - type: file - description: Plots created by MultiQC - pattern: "*_data" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@abhi18av" - - "@bunop" - - "@drpatelh" - - "@jfy133" -maintainers: - - "@abhi18av" - - "@bunop" - - "@drpatelh" - - "@jfy133" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test deleted file mode 100644 index f1c4242..0000000 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ /dev/null @@ -1,84 +0,0 @@ -nextflow_process { - - name "Test Process MULTIQC" - script "../main.nf" - process "MULTIQC" - - tag "modules" - tag "modules_nfcore" - tag "multiqc" - - test("sarscov2 single-end [fastqc]") { - - when { - process { - """ - input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) - input[1] = [] - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, - { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("multiqc_versions_single") } - ) - } - - } - - test("sarscov2 single-end [fastqc] [config]") { - - when { - process { - """ - input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) - input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, - { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("multiqc_versions_config") } - ) - } - } - - test("sarscov2 single-end [fastqc] - stub") { - - options "-stub" - - when { - process { - """ - input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) - input[1] = [] - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out.report.collect { file(it).getName() } + - process.out.data.collect { file(it).getName() } + - process.out.plots.collect { file(it).getName() } + - process.out.versions ).match("multiqc_stub") } - ) - } - - } -} diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap deleted file mode 100644 index bfebd80..0000000 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ /dev/null @@ -1,41 +0,0 @@ -{ - "multiqc_versions_single": { - "content": [ - [ - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-29T08:48:55.657331" - }, - "multiqc_stub": { - "content": [ - [ - "multiqc_report.html", - "multiqc_data", - "multiqc_plots", - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-29T08:49:49.071937" - }, - "multiqc_versions_config": { - "content": [ - [ - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-29T08:49:25.457567" - } -} \ No newline at end of file diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml deleted file mode 100644 index bea6c0d..0000000 --- a/modules/nf-core/multiqc/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -multiqc: - - modules/nf-core/multiqc/** diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test b/modules/nf-core/samtools/flagstat/tests/main.nf.test index 24c3c04..3b648a3 100644 --- a/modules/nf-core/samtools/flagstat/tests/main.nf.test +++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test @@ -11,9 +11,30 @@ nextflow_process { test("BAM") { when { - params { - outdir = "$outputDir" + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true) + ]) + """ } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("BAM - stub") { + + options "-stub" + + when { process { """ input[0] = Channel.of([ @@ -28,8 +49,7 @@ nextflow_process { then { assertAll ( { assert process.success }, - { assert snapshot(process.out.flagstat).match("flagstat") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out).match() } ) } } diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap index e9f85ef..23989c6 100644 --- a/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap @@ -1,32 +1,72 @@ { - "flagstat": { + "BAM - stub": { "content": [ - [ - [ - { - "id": "test", - "single_end": false - }, - "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783" + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,f606681ef971cbb548a4d9e3fbabdbc2" + ], + "flagstat": [ + [ + { + "id": "test", + "single_end": false + }, + "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,f606681ef971cbb548a4d9e3fbabdbc2" ] - ] + } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.04.3" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-02-12T18:31:37.783927" + "timestamp": "2024-07-22T14:17:28.002887" }, - "versions": { + "BAM": { "content": [ - [ - "versions.yml:md5,f606681ef971cbb548a4d9e3fbabdbc2" - ] + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783" + ] + ], + "1": [ + "versions.yml:md5,f606681ef971cbb548a4d9e3fbabdbc2" + ], + "flagstat": [ + [ + { + "id": "test", + "single_end": false + }, + "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783" + ] + ], + "versions": [ + "versions.yml:md5,f606681ef971cbb548a4d9e3fbabdbc2" + ] + } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-05-28T15:41:52.516253882" + "timestamp": "2024-07-22T14:17:13.330971" } } \ No newline at end of file diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test b/modules/nf-core/samtools/idxstats/tests/main.nf.test index a2dcb27..5fd1fc7 100644 --- a/modules/nf-core/samtools/idxstats/tests/main.nf.test +++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test @@ -11,9 +11,6 @@ nextflow_process { test("bam") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([ @@ -28,9 +25,29 @@ nextflow_process { then { assertAll ( { assert process.success }, - { assert snapshot(process.out.idxstats).match("idxstats") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out).match() } ) } } -} + + test("bam - stub") { + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + }} diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap index 4eacdb9..a5ac810 100644 --- a/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap @@ -1,32 +1,72 @@ { - "versions": { + "bam - stub": { "content": [ - [ - "versions.yml:md5,7acbcb2a8ec6436ba7b2916d3ff13351" - ] + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,7acbcb2a8ec6436ba7b2916d3ff13351" + ], + "idxstats": [ + [ + { + "id": "test", + "single_end": false + }, + "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,7acbcb2a8ec6436ba7b2916d3ff13351" + ] + } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-05-28T15:46:46.617989517" + "timestamp": "2024-07-22T14:17:56.180093" }, - "idxstats": { + "bam": { "content": [ - [ - [ - { - "id": "test", - "single_end": false - }, - "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2" + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2" + ] + ], + "1": [ + "versions.yml:md5,7acbcb2a8ec6436ba7b2916d3ff13351" + ], + "idxstats": [ + [ + { + "id": "test", + "single_end": false + }, + "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2" + ] + ], + "versions": [ + "versions.yml:md5,7acbcb2a8ec6436ba7b2916d3ff13351" ] - ] + } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.04.3" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-02-12T18:36:41.561026" + "timestamp": "2024-07-22T14:17:41.408704" } } \ No newline at end of file diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test b/modules/nf-core/samtools/stats/tests/main.nf.test index e3d5cb1..5bc8930 100644 --- a/modules/nf-core/samtools/stats/tests/main.nf.test +++ b/modules/nf-core/samtools/stats/tests/main.nf.test @@ -3,6 +3,7 @@ nextflow_process { name "Test Process SAMTOOLS_STATS" script "../main.nf" process "SAMTOOLS_STATS" + tag "modules" tag "modules_nfcore" tag "samtools" @@ -11,9 +12,6 @@ nextflow_process { test("bam") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([ @@ -37,9 +35,59 @@ nextflow_process { test("cram") { when { - params { - outdir = "$outputDir" + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true) + ]) + """ } + } + + then { + assertAll( + {assert process.success}, + {assert snapshot(process.out).match()} + ) + } + } + + test("bam - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true) + ]) + input[1] = [[],[]] + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert snapshot(process.out).match()} + ) + } + } + + test("cram - stub") { + + options "-stub" + + when { process { """ input[0] = Channel.of([ @@ -49,7 +97,7 @@ nextflow_process { ]) input[1] = Channel.of([ [ id:'genome' ], // meta map - file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true) ]) """ } diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test.snap b/modules/nf-core/samtools/stats/tests/main.nf.test.snap index 2747fd6..3828f37 100644 --- a/modules/nf-core/samtools/stats/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/stats/tests/main.nf.test.snap @@ -29,10 +29,80 @@ } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-05-28T15:45:24.403941966" + "timestamp": "2024-07-22T14:20:24.885816" + }, + "bam - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,b3b70b126f867fdbb7dcea5e36e49d4a" + ], + "stats": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,b3b70b126f867fdbb7dcea5e36e49d4a" + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T14:20:39.310713" + }, + "cram - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,b3b70b126f867fdbb7dcea5e36e49d4a" + ], + "stats": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,b3b70b126f867fdbb7dcea5e36e49d4a" + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T14:21:04.771199" }, "bam": { "content": [ @@ -64,9 +134,9 @@ } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-05-28T15:45:06.711251947" + "timestamp": "2024-07-22T14:19:06.645466" } } \ No newline at end of file From 198de2a8bb4fc025b8018e3d62742e66d49b29fd Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Tue, 20 Aug 2024 09:28:06 +0100 Subject: [PATCH 102/111] add new dependencies and parameters --- CHANGELOG.md | 15 +++++++++------ 1 file changed, 9 insertions(+), 6 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 339afc1..80e60f5 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## [[1.3.0](https://github.com/sanger-tol/readmapping/releases/tag/1.3.0)] - Antipodean Opaleye - [2024-08-19] +## [[1.3.0](https://github.com/sanger-tol/readmapping/releases/tag/1.3.0)] - Antipodean Opaleye - [2024-08-20] ### Enhancements & fixes @@ -21,8 +21,11 @@ Note, since the pipeline is using Nextflow DSL2, each process will be run with i | Dependency | Old version | New version | | ---------- | ------------- | ------------- | -| `blastn` | 2.13 | 2.15 | -| `samtools` | 1.14 and 1.17 | 1.17 and 1.18 | +| `blastn` | 2.13 | 2.15 | +| `minimap2` | 2.24 | 2.28 | +| `samtools` | 1.14 and 1.17 | 1.20 | +| `seqkit` | | 2.8.1 | +| `seqtk` | | 1.4 | > **NB:** Dependency has been **updated** if both old and new version information is present.
    **NB:** Dependency has been **added** if just the new version information is present.
    **NB:** Dependency has been **removed** if version information isn't present. @@ -30,9 +33,9 @@ Note, since the pipeline is using Nextflow DSL2, each process will be run with i | Old parameter | New parameter | | ------------------ | ------------- | -| `--enable_conda` | | -| `--minimap2_index` | | -| `--samtools_index` | | +| | '--header' | +| | '--outfmt' | +| | '--compression| > **NB:** Parameter has been **updated** if both old and new parameter information is present.
    **NB:** Parameter has been **added** if just the new parameter information is present.
    **NB:** Parameter has been **removed** if new parameter information isn't present. From cd2c5547fdf0c21438c5bd1e827e54587d945516 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Tue, 20 Aug 2024 10:51:23 +0100 Subject: [PATCH 103/111] Vernon 1.3.0 --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 85e8051..bab0aa1 100644 --- a/nextflow.config +++ b/nextflow.config @@ -231,7 +231,7 @@ manifest { description = 'Pipeline to map reads generated using different sequencing technologies against a genome assembly.' mainScript = 'main.nf' nextflowVersion = '!>=22.10.1' - version = 'dev: 1.3.0' + version = '1.3.0' doi = '10.5281/zenodo.6563577' } From 906d6dc8a0952c339d3930b1d91b64a21216ff4d Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Tue, 20 Aug 2024 10:53:36 +0100 Subject: [PATCH 104/111] Update CITATIONS.md --- CITATIONS.md | 20 +++++++++++++------- 1 file changed, 13 insertions(+), 7 deletions(-) diff --git a/CITATIONS.md b/CITATIONS.md index 4e1340d..6c5d67a 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -1,4 +1,4 @@ -# nf-core/readmapping: Citations +# sanger-tol/readmapping: Citations ## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) @@ -10,13 +10,21 @@ ## Pipeline tools -- [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) +- [Blast](https://pubmed.ncbi.nlm.nih.gov/20003500/) - > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. + > Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K, Madden TL. BLAST+: architecture and applications. BMC Bioinformatics. 2009 Dec 15;10:421. doi: 10.1186/1471-2105-10-421. PMID: 20003500; PMCID: PMC2803857. -- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) +- [BWA-MEM2](https://ieeexplore.ieee.org/document/8820962) - > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. + > Vasimuddin Md, Misra S, Li H, Aluru S. Efficient Architecture-Aware Acceleration of BWA-MEM for Multicore Systems. 2019 IEEE International Parallel and Distributed Processing Symposium. 2019 May;314–24. doi: 10.1109/IPDPS.2019.00041. + +- [Minimap2](https://pubmed.ncbi.nlm.nih.gov/34623391/) + + > Li H. New strategies to improve minimap2 alignment accuracy. Bioinformatics. 2021 Oct 8;37(23):4572–4. doi: 10.1093/bioinformatics/btab705. Epub ahead of print. PMID: 34623391; PMCID: PMC8652018. + +- [Samtools](https://pubmed.ncbi.nlm.nih.gov/33590861/) + + > Danecek P, Bonfield JK, Liddle J, Marshall J, Ohan V, Pollard MO, Whitwham A, Keane T, McCarthy SA, Davies RM, Li H. Twelve years of SAMtools and BCFtools. Gigascience. 2021 Feb 16;10(2):giab008. doi: 10.1093/gigascience/giab008. PMID: 33590861; PMCID: PMC7931819. ## Software packaging/containerisation tools @@ -34,8 +42,6 @@ - [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241) - > Merkel, D. (2014). Docker: lightweight linux containers for consistent development and deployment. Linux Journal, 2014(239), 2. doi: 10.5555/2600239.2600241. - - [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/) > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675. From d2ed0438ad3bd642f59d9d63bc525c0ca63ef14c Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Tue, 20 Aug 2024 12:48:50 +0100 Subject: [PATCH 105/111] ignore missing tower.yml --- .nf-core.yml | 1 + 1 file changed, 1 insertion(+) diff --git a/.nf-core.yml b/.nf-core.yml index 6d9e4e4..8180bc8 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -14,6 +14,7 @@ lint: - .github/ISSUE_TEMPLATE/config.yml - .github/workflows/awstest.yml - .github/workflows/awsfulltest.yml + - tower.yml files_unchanged: - LICENSE - .github/CONTRIBUTING.md From a4ac213a3436baf4b018caf44a95af53a546b6d9 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Tue, 20 Aug 2024 12:49:00 +0100 Subject: [PATCH 106/111] remove unnedded tower.yml --- tower.yml | 5 ----- 1 file changed, 5 deletions(-) delete mode 100644 tower.yml diff --git a/tower.yml b/tower.yml deleted file mode 100644 index 787aedf..0000000 --- a/tower.yml +++ /dev/null @@ -1,5 +0,0 @@ -reports: - multiqc_report.html: - display: "MultiQC HTML report" - samplesheet.csv: - display: "Auto-created samplesheet with collated metadata and FASTQ paths" From 9e6803d430e021e687da403e7739522c97166a23 Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Tue, 20 Aug 2024 13:09:18 +0100 Subject: [PATCH 107/111] added new tool citations --- CITATIONS.md | 16 +++++++++++++++- 1 file changed, 15 insertions(+), 1 deletion(-) diff --git a/CITATIONS.md b/CITATIONS.md index 6c5d67a..4a33c7c 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -6,7 +6,7 @@ ## [Nextflow](https://pubmed.ncbi.nlm.nih.gov/28398311/) -> Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311. +> ## Pipeline tools @@ -18,6 +18,10 @@ > Vasimuddin Md, Misra S, Li H, Aluru S. Efficient Architecture-Aware Acceleration of BWA-MEM for Multicore Systems. 2019 IEEE International Parallel and Distributed Processing Symposium. 2019 May;314–24. doi: 10.1109/IPDPS.2019.00041. +- [CRUMBLE] + + > Bonfield JK, McCarthy SA, Durbin R. Crumble: reference free lossy compression of sequence quality values. Bioinformatics. 2019 Jan;35(2):337-339. doi: 10.1093/bioinformatics/bty608. PubMed PMID: 29992288; PMCID: PMC6330002. + - [Minimap2](https://pubmed.ncbi.nlm.nih.gov/34623391/) > Li H. New strategies to improve minimap2 alignment accuracy. Bioinformatics. 2021 Oct 8;37(23):4572–4. doi: 10.1093/bioinformatics/btab705. Epub ahead of print. PMID: 34623391; PMCID: PMC8652018. @@ -26,6 +30,14 @@ > Danecek P, Bonfield JK, Liddle J, Marshall J, Ohan V, Pollard MO, Whitwham A, Keane T, McCarthy SA, Davies RM, Li H. Twelve years of SAMtools and BCFtools. Gigascience. 2021 Feb 16;10(2):giab008. doi: 10.1093/gigascience/giab008. PMID: 33590861; PMCID: PMC7931819. +- [SeqKit] + + > Shen W, Le S, Li Y, Hu F. SeqKit: A cross-platform and ultrafast toolkit for FASTA/Q file manipulation. PLoS One. 2016 Oct 5;11(10):e0163962. doi: 10.1371/journal.pone.0163962. PubMed PMID: 27706213; PMCID: PMC5051824. + +- [Seqtk] + + > Li H. Toolkit for processing sequences in FASTA/Q formats. GitHub Repository. 2012. https://github.com/lh3/seqtk. Accessed August 2024. + ## Software packaging/containerisation tools - [Anaconda](https://anaconda.com) @@ -42,6 +54,8 @@ - [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241) + > Merkel, D. Docker: lightweight linux containers for consistent development and deployment. Linux Journal, 2014 Mar;2014(239):2. doi: 10.5555/2600239.2600241. + - [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/) > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675. From e189fe4796d599f82e58b842295ffd3e5d5e738f Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Tue, 20 Aug 2024 13:23:11 +0100 Subject: [PATCH 108/111] prettier linting --- CHANGELOG.md | 26 +++++++++++++------------- 1 file changed, 13 insertions(+), 13 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 80e60f5..31c9066 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,7 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Combined steps to improve the efficiency of the pipeline, especially on large genomes - "crumble" is now run on _every_ data type, not just PacBio - Added options for output format and to turn on/off crumble compression (https://github.com/sanger-tol/readmapping/pull/107) -- Added `fastq` as possible input type for PacBio (https://github.com/sanger-tol/readmapping/pull/106) and Illumina/HiC (https://github.com/sanger-tol/readmapping/pull/96) +- Added `fastq` as possible input type for PacBio (https://github.com/sanger-tol/readmapping/pull/106) and Illumina/HiC (https://github.com/sanger-tol/readmapping/pull/96) - Disabled running `bwa index` when no short-read data provided (https://github.com/sanger-tol/readmapping/pull/100) - Added support for optional custom SAM header (https://github.com/sanger-tol/readmapping/pull/95) - Switch to `nf-validation` (https://github.com/sanger-tol/readmapping/pull/99) and further updates for nf-core v2.14 compliance (https://github.com/sanger-tol/readmapping/pull/98) @@ -19,23 +19,23 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 Note, since the pipeline is using Nextflow DSL2, each process will be run with its own [Biocontainer](https://biocontainers.pro/#/registry). This means that on occasion it is entirely possible for the pipeline to be using different versions of the same tool. However, the overall software dependency changes compared to the last release have been listed below for reference. -| Dependency | Old version | New version | -| ---------- | ------------- | ------------- | -| `blastn` | 2.13 | 2.15 | -| `minimap2` | 2.24 | 2.28 | -| `samtools` | 1.14 and 1.17 | 1.20 | -| `seqkit` | | 2.8.1 | -| `seqtk` | | 1.4 | +| Dependency | Old version | New version | +| ---------- | ------------- | ----------- | +| `blastn` | 2.13 | 2.15 | +| `minimap2` | 2.24 | 2.28 | +| `samtools` | 1.14 and 1.17 | 1.20 | +| `seqkit` | | 2.8.1 | +| `seqtk` | | 1.4 | > **NB:** Dependency has been **updated** if both old and new version information is present.
    **NB:** Dependency has been **added** if just the new version information is present.
    **NB:** Dependency has been **removed** if version information isn't present. ### Parameters -| Old parameter | New parameter | -| ------------------ | ------------- | -| | '--header' | -| | '--outfmt' | -| | '--compression| +| Old parameter | New parameter | +| ------------- | -------------- | +| | '--header' | +| | '--outfmt' | +| | '--compression | > **NB:** Parameter has been **updated** if both old and new parameter information is present.
    **NB:** Parameter has been **added** if just the new parameter information is present.
    **NB:** Parameter has been **removed** if new parameter information isn't present. From a141819a27049e2c3386ed3b6ccf8fd16129c7bd Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Thu, 22 Aug 2024 09:27:20 +0100 Subject: [PATCH 109/111] new date --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 31c9066..fb82319 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## [[1.3.0](https://github.com/sanger-tol/readmapping/releases/tag/1.3.0)] - Antipodean Opaleye - [2024-08-20] +## [[1.3.0](https://github.com/sanger-tol/readmapping/releases/tag/1.3.0)] - Antipodean Opaleye - [2024-08-23] ### Enhancements & fixes From a31b253eeef58246dcd67d4336f9e78699556c9d Mon Sep 17 00:00:00 2001 From: Matthieu Muffato Date: Thu, 22 Aug 2024 08:56:42 +0100 Subject: [PATCH 110/111] Fixed a few occurrences of "nf-core" --- CHANGELOG.md | 4 ++-- assets/slackreport.json | 2 +- modules.json | 4 ++-- nextflow.config | 4 ++-- 4 files changed, 7 insertions(+), 7 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index fb82319..ca9294f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,4 +1,4 @@ -# nf-core/readmapping: Changelog +# sanger-tol/readmapping: Changelog The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). @@ -129,7 +129,7 @@ Note, since the pipeline is using Nextflow DSL2, each process will be run with i ## [[v1.0.0](https://github.com/sanger-tol/readmapping/releases/tag/1.0.0)] – Ukrainian Ironbelly - [2022-05-19] -Initial release of nf-core/readmapping, created with the [nf-core](https://nf-co.re/) template. +Initial release of sanger-tol/readmapping, created with the [nf-core](https://nf-co.re/) template. ### `Added` diff --git a/assets/slackreport.json b/assets/slackreport.json index 0eb4bdb..d3754ad 100644 --- a/assets/slackreport.json +++ b/assets/slackreport.json @@ -3,7 +3,7 @@ { "fallback": "Plain-text summary of the attachment.", "color": "<% if (success) { %>good<% } else { %>danger<%} %>", - "author_name": "nf-core/readmapping ${version} - ${runName}", + "author_name": "sanger-tol/readmapping ${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/modules.json b/modules.json index bcabde0..b7bb93d 100644 --- a/modules.json +++ b/modules.json @@ -1,6 +1,6 @@ { - "name": "nf-core/readmapping", - "homePage": "https://github.com/nf-core/readmapping", + "name": "sanger-tol/readmapping", + "homePage": "https://github.com/sanger-tol/readmapping", "repos": { "https://github.com/nf-core/modules.git": { "modules": { diff --git a/nextflow.config b/nextflow.config index bab0aa1..d9c7344 100644 --- a/nextflow.config +++ b/nextflow.config @@ -1,6 +1,6 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - nf-core/readmapping Nextflow config file + sanger-tol/readmapping Nextflow config file ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Default config options for all compute environments ---------------------------------------------------------------------------------------- @@ -72,7 +72,7 @@ try { System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") } -// Load nf-core/readmapping custom profiles from different institutions. +// Load sanger-tol/readmapping custom profiles from different institutions. profiles { debug { dumpHashes = true From f4418b7b109f362f850394e6709d8264319079fa Mon Sep 17 00:00:00 2001 From: Tyler Chafin Date: Thu, 22 Aug 2024 15:28:51 +0100 Subject: [PATCH 111/111] revert deleted profile --- nextflow.config | 1 + 1 file changed, 1 insertion(+) diff --git a/nextflow.config b/nextflow.config index d9c7344..437ff82 100644 --- a/nextflow.config +++ b/nextflow.config @@ -74,6 +74,7 @@ try { // Load sanger-tol/readmapping custom profiles from different institutions. profiles { + cleanup { cleanup = true } debug { dumpHashes = true process.beforeScript = 'echo $HOSTNAME'