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    • An m6A-aware basecalling model to detect m6A modifications at single nucleotide resolution in individual reads (Cruciani, Delgado-Tejedor, Pryszcz et al., BioRxiv 2023)
      Jupyter Notebook
      GNU General Public License v3.0
      11520Updated Dec 16, 2024Dec 16, 2024
    • SeqTagger

      Public
      Super-fast and accurate demultiplexing of direct RNA-seq runs.
      Jupyter Notebook
      Other
      1410Updated Dec 5, 2024Dec 5, 2024
    • Poly-A tail length estimation from Nano3P-seq libraries
      Jupyter Notebook
      0110Updated Nov 11, 2024Nov 11, 2024
    • Scripts used Milenkovic et al. "Epitranscriptomic rRNA fingerprinting reveals tissue-of-origin and tumor-specific signatures" (Mol Cell 2024)
      R
      0000Updated Nov 8, 2024Nov 8, 2024
    • Quantitative analysis of native tRNA populations using direct RNA nanopore sequencing (Lucas*, Pryszcz* et al., Nat Biotech 2023)
      Python
      GNU General Public License v3.0
      51420Updated Sep 19, 2024Sep 19, 2024
    • EpiNano

      Public
      Detection of RNA modifications from Oxford Nanopore direct RNA sequencing reads (Liu*, Begik* et al., Nature Comm 2019)
      Python
      GNU General Public License v2.0
      3111331Updated Jun 30, 2024Jun 30, 2024
    • Benchmarked base calling models for nanopore sequencing providing super high accuracy and improved RNA modification detection
      R
      MIT License
      0500Updated May 9, 2024May 9, 2024
    • From samtools mpileup to stats of coverage, ref_nuc, mismatches, deletions, insertions, RT-stops
      Perl
      MIT License
      0000Updated May 8, 2024May 8, 2024
    • modPhred

      Public
      modPhred is a pipeline for detection of DNA/RNA modifications from raw ONT data
      Python
      MIT License
      11620Updated Apr 29, 2024Apr 29, 2024
    • Detection of Nm sites from Nanopore DRS data
      R
      0100Updated Mar 27, 2024Mar 27, 2024
    • Direct RNA sequencing (DRS) analysis included in article: N6-methyladenosine modification is not a general trait of viral RNA genomes
      Shell
      0000Updated Mar 15, 2024Mar 15, 2024
    • Nanopore 3' end-capture sequencing (Begik et al., bioRxiv 2021)
      Python
      MIT License
      31210Updated Jul 18, 2023Jul 18, 2023
    • RPL3L

      Public
      Scripts used to produce plots in the RPL3L manuscript
      HTML
      0000Updated Mar 13, 2023Mar 13, 2023
    • R
      MIT License
      0100Updated Mar 7, 2023Mar 7, 2023
    • nanoRMS

      Public
      Prediction of RNA modifications and their stoichiometry from per-read features: current intensity, dwell time and trace (Begik*, Lucas* et al., Nature Biotech 2021)
      Jupyter Notebook
      102321Updated Dec 21, 2022Dec 21, 2022
    • Best practices for the Analysis of Oxford Nanopore Direct RNA sequencing Data (Begik*, Lucas*, et al., bioRxiv 2020)
      R
      1910Updated Dec 16, 2022Dec 16, 2022
    • original hoffmann pipeline, with changes for portability
      Perl
      0000Updated Dec 16, 2022Dec 16, 2022
    • Analyses from manuscript: Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signatures (Begik et al. Genome Biology 2020).
      R
      3200Updated Dec 16, 2022Dec 16, 2022
    • Compute codon autocorrelation from fasta CDS sequences (Novoa et al., Mol Biol Evol 2019)
      R
      MIT License
      5200Updated Dec 16, 2022Dec 16, 2022
    • Analysis of Direct RNA Sequencing of Yeast Ribosomal RNA
      R
      1310Updated Dec 16, 2022Dec 16, 2022
    • lab_website

      Public archive
      Website for NovoaLab, built by the NovoaLab team
      CSS
      MIT License
      0000Updated Jun 14, 2022Jun 14, 2022
    • 0000Updated May 5, 2022May 5, 2022
    • tombo

      Public archive
      Tombo is a suite of tools primarily for the identification of modified nucleotides from raw nanopore sequencing data.
      Python
      Other
      54000Updated Nov 11, 2021Nov 11, 2021
    • Markdown Cheatsheet for Github Readme.md
      MIT License
      6.4k000Updated Mar 22, 2021Mar 22, 2021
    • deepTools

      Public
      Tools to process and analyze deep sequencing data.
      Python
      Other
      212000Updated May 2, 2020May 2, 2020
    • Nextflow pipeline for analysis of direct RNA Nanopore reads (Cozzuto et al., Front in Genet 2020)
      HTML
      MIT License
      16100Updated Apr 6, 2020Apr 6, 2020
    • Signal based nanopore RNA demultiplexing with convolutional neural networks (Smith*, Ersavas*, Ferguson* et al., Genome Research 2020)
      Jupyter Notebook
      MIT License
      8000Updated Jan 17, 2020Jan 17, 2020
    • riboflow

      Public
      Pipeline for Ribosome Profiling Data
      Groovy
      MIT License
      9000Updated Dec 20, 2019Dec 20, 2019
    • SquiggleKit: A toolkit for manipulating nanopore signal data
      Python
      MIT License
      23100Updated Dec 2, 2019Dec 2, 2019
    • Scripts for identifying sites with differential error rates in mapped nanopore DRS data
      Python
      3100Updated Aug 13, 2019Aug 13, 2019