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MultiQC Version History

MultiQC v1.6dev

Some of these updates are thanks to the efforts of people who attended the NASPM 2018 MultiQC hackathon session. Thanks to everyone who attended!

New Modules:

  • fastp
    • An ultra-fast all-in-one FASTQ preprocessor (QC, adapters, trimming, filtering, splitting...)
    • Module started by @florianduclot and completed by @ewels
  • Long Ranger
    • Works with data from the 10X Genomics Chromium. Performs sample demultiplexing, barcode processing, alignment, quality control, variant calling, phasing, and structural variant calling.
    • Module written by @remiolsen

Module updates:

  • BCFtools
  • BBMap
    • Support added for BBDuk kmer-based adapter/contaminant filtering summary stats (@boulund
  • FastQC
    • New read count plot, split into unique and duplicate reads if possible.
    • Help text added for all sections, mostly copied from the excellent FastQC help.
  • FastQ Screen
    • Samples in large-sample-number plot are now sorted alphabetically (@hassanfa
  • MACS2
    • Output is now more tolerant of missing data (no plot if no data)
  • Peddy
    • Background samples now shown in ancestry PCA plot (@roryk)
    • New plot showing sex checks versus het ratios, supporting unknowns (@oyvinev)
  • Picard
    • New submodule to handle ValidateSamFile reports (@cpavanrun)
    • WGSMetrics now add the mean and standard-deviation coverage to the general stats table (hidden) (@cpavanrun)
  • QUAST
    • Null values (-) in reports now handled properly. Bargraphs always shown despite varying thresholds. (@vladsaveliev)
  • RNA-SeQC
    • Don't create the report section for Gene Body Coverage if no data is given
  • Samtools
    • Fixed edge case bug where MultiQC could crash if a sample had zero count coverage with idxstats.
  • Tophat
    • Fixed bug where some samples could be given a blank sample name (@lparsons)
  • VerifyBamID
    • Change column header help text for contamination to match percentage output (@chapmanb)

New MultiQC Features:

  • Add path_filters_exclude to exclude certain files when running modules multiple times. You could previously only include certain files.
  • New exclude_* keys for file search patterns
    • Have a subset of patterns to exclude otherwise detected files with, by filename or contents
  • Command line options all now use mid-word hyphens (not a mix of hyphens and underscores)
    • Old underscore terms still maintained for backwards compatibility
  • Flag --view-tags now works without requiring an "analysis directory".
  • Removed Python dependency for enum34 (@boulund)
  • Columns can be added to General Stats table for custom content/module.
  • New --ignore-symlinks flag which will ignore symlinked directories and files.
  • New --no-megaqc-upload flag which disables automatically uploading data to MegaQC

Bug Fixes

  • Fix path_filters for top_modules/module_order configuration only selecting if all globs match. It now filters searches that match any glob.
  • Empty sample names from cleaning are now no longer allowed
  • Stop prepend_dirs set in the config from getting clobbered by an unpassed CLI option (@tsnowlan)
  • Modules running multiple times now have multiple sets of columns in the General Statistics table again, instead of overwriting one another.
  • Prevent tables from clobbering sorted row orders.
  • Fix linegraph and scatter plots data conversion (sporadically the incorrect ymax was used to drop data points) (@cpavanrun)
  • Adjusted multiple file search patterns to make them more specific
    • Prevents the wrong module from accidentally slurping up output from a different tool. By @cpavanrun (see PR #727)

MultiQC v1.5 - 2018-03-15

New Modules:

  • HiCPro - New module!
    • HiCPro: Quality controls and processing of Hi-C
    • Module written by @nservant,
  • DeDup - New module!
    • DeDup: Improved Duplicate Removal for merged/collapsed reads in ancient DNA analysis
    • Module written by @apeltzer,
  • Clip&Merge - New module!
    • Clip&Merge: Adapter clipping and read merging for ancient DNA analysis
    • Module written by @apeltzer,

Module updates:

  • bcl2fastq
    • Catch ZeroDivisionError exceptions when there are 0 reads (@aledj2)
    • Add parsing of TrimmedBases and new General Stats column for % bases trimmed (@matthdsm).
  • BUSCO
    • Fixed configuration bug that made all sample names become 'short'
  • Custom Content
    • Parsed tables now exported to multiqc_data files
  • Cutadapt
    • Refactor parsing code to collect all length trimming plots
  • FastQC
    • Fixed starting y-axis label for GC-content lineplot being incorrect.
  • HiCExplorer
    • Updated to work with v2.0 release.
  • Homer
    • Made parsing of tagInfo.txt file more resilient to variations in file format so that it works with new versions of Homer.
    • Kept order of chromosomes in coverage plot consistent.
  • Peddy
    • Switch Sex error logic to Correct sex for better highlighting (@aledj2)
  • Picard
    • Updated module and search patterns to recognise new output format from Picard version >= 2.16 and GATK output.
  • Qualimap BamQC
    • Fixed bug where start of Genome Fraction could have a step if target is 100% covered.
  • RNA-SeQC
    • Added rRNA alignment stats to summary table @Rolandde
  • RSeqC
    • Fixed read distribution plot by adding category for other_intergenic (thanks to @moxgreen)
    • Fixed a dodgy plot title (Read GC content)
  • Supernova
    • Added support for Supernova 2.0 reports. Fixed a TypeError bug when using txt reports only. Also a bug when parsing empty histogram files.

New MultiQC Features:

  • Invalid choices for --module or --exclude now list the available modules alphabetically.
  • Linting now checks for presence in config.module_order and tags.

Bug Fixes

  • Excluding modules now works in combination with using module tags.
  • Fixed edge-case bug where certain combinations of output_fn_name and data_dir_name could trigger a crash
  • Conditional formatting - values are now longer double-labelled
  • Made config option extra_series work in scatter plots the same way that it works for line plots
  • Locked the matplotlib version to v2.1.0 and below
    • Due to two bugs that appeared in v2.2.0 - will remove this constraint when there's a new release that works again.

MultiQC v1.4 - 2018-01-11

A slightly earlier-than-expected release due to a new problem with dependency packages that is breaking MultiQC installations since 2018-01-11.

New Modules:

  • Sargasso
    • Parses output from Sargasso - a tool to separate mixed-species RNA-seq reads according to their species of origin
    • Module written by @hxin
  • VerifyBAMID
    • Parses output from VerifyBAMID - a tool to detect contamination in BAM files.
    • Adds the CHIPMIX and FREEMIX columns to the general statistics table.
    • Module written by @aledj2

Module updates:

  • MACS2
    • Updated to work with output from older versions of MACS2 by @avilella
  • Peddy
    • Add het check plot to suggest potential contamination by @aledj2
  • Picard
    • Picard HsMetrics HS_PENALTY plot now has correct axis labels
    • InsertSizeMetrics switches commas for points if it can't convert floats. Should help some european users.
  • QoRTs
    • Added support for new style of output generated in the v1.3.0 release
  • Qualimap
    • New Error rate column in General Statistics table, added by @Cashalow
      • Hidden by default - customise your MultiQC config to always show this column (see docs)
  • QUAST
    • New option to customise the default display of contig count and length (eg. bp instead of Mbp).
    • See documentation. Written by @ewels and @Cashalow
  • RSeQC
    • Removed normalisation in Junction Saturation plot. Now raw counts instead of % of total junctions.

New MultiQC Features:

  • Conditional formatting / highlighting of cell contents in tables
    • If you want to make values that match a criteria stand out more, you can now write custom rules and formatting instructions for tables.
    • For instructions, see the documentation
  • New --lint option which is strict about best-practices for writing new modules
    • Useful when writing new modules and code as it throws warnings
    • Currently only implemented for bar plots and a few other places. More linting coming soon...
  • If MultiQC breaks and shows am error message, it now reports the filename of the last log it found
    • Hopefully this will help with debugging / finding dodgy input data

Bug Fixes

  • Addressed new dependency error with conflicting package requirements
    • There was a conflict between the networkx, colormath and spectra releases.
    • I previously forced certain software versions to get around this, but spectra has now updated with the unfortunate effect of introducing a new dependency clash that halts installation.
  • Fixed newly introduced bug where Custom Content MultiQC config file search patterns had been broken
  • Updated pandoc command used in --pdf to work with new releases of Pandoc
  • Made config table_columns_visible module name key matching case insensitive to make less frustrating

MultiQC v1.3 - 2017-11-03

Breaking changes - custom search patterns

Only for users with custom search patterns for the bowtie or star: you will need to update your config files - the bowtie search key is now bowtie1, star_genecounts is now star/genecounts.

For users with custom modules - search patterns must now conform to the search pattern naming convention: modulename or modulename/anything (the search pattern string beginning with the name of your module, anything you like after the first /).

New Modules:

  • 10X Supernova
    • Parses statistics from the de-novo Supernova software.
    • Module written by @remiolsen
  • BBMap
    • Plot metrics from a number of BBMap tools, a suite of DNA/RNA mapping tools and utilities
    • Module written by @boulund and @epruesse
  • deepTools - new module!
    • Parse text output from bamPEFragmentSize, estimateReadFiltering, plotCoverage, plotEnrichment, and plotFingerprint
    • Module written by @dpryan79
  • Homer Tag Directory - new submodule!
  • illumina InterOp
    • Module to parse metrics from illumina sequencing runs and demultiplexing, generated by the InterOp package
    • Module written by @matthdsm
  • RSEM - new module!
    • Parse .cnt file comming from rsem-calculate-expression and plot read repartitions (Unalignable, Unique, Multi ...)
    • Module written by @noirot
  • HiCExplorer
    • New module to parse the log files of hicBuildMatrix.
    • Module written by @joachimwolff

Module updates:

  • AfterQC
    • Handle new output format where JSON summary key changed names.
  • bcl2fastq
    • Clusters per sample plot now has tab where counts are categoried by lane.
  • GATK
    • New submodule to handle Base Recalibrator stats, written by @winni2k
  • HiSAT2
    • Fixed bug where plot title was incorrect if both SE and PE bargraphs were in one report
  • Picard HsMetrics
    • Parsing code can now handle commas for decimal places
  • Preseq
    • Updated odd file-search pattern that limited input files to 500kb
  • QoRTs
    • Added new plots, new helptext and updated the module to produce a lot more output.
  • Qualimap BamQC
    • Fixed edge-case bug where the refactored coverage plot code could raise an error from the range call.
  • Documentation and link fixes for Slamdunk, GATK, bcl2fastq, Adapter Removal, FastQC and main docs
  • Went through all modules and standardised plot titles
    • All plots should now have a title with the format Module name: Plot name

New MultiQC Features:

  • New MultiQC docker image
  • New module_order config options allow modules to be run multiple times
    • Filters mean that a module can be run twice with different sets of files (eg. before and after trimming)
    • Custom module config parameters can be passed to module for each run
  • File search refactored to only search for running modules
    • Makes search much faster when running with lots of files and limited modules
    • For example, if using -m star to only use the STAR module, all other file searches now skipped
  • File search now warns if an unrecognised search type is given
  • MultiQC now saves nearly all parsed data to a structured output file by default
    • See multiqc_data/multiqc_data.json
    • This can be turned off by setting config.data_dump_file: false
  • Verbose logging when no log files found standardised. Less duplication in code and logs easier to read!
  • New documentation section describing how to use MultiQC with Galaxy
  • Using shared_key: 'read_counts' in table header configs now applies relevant defaults

Bug Fixes

  • Installation problem caused by changes in upstream dependencies solved by stricter installation requirements
  • Minor default_dev directory creation bug squashed
  • Don't prepend the directory separator (|) to sample names with -d when there are no subdirs
  • yPlotLines now works even if you don't set width

MultiQC v1.2 - 2017-08-16

CodeFest 2017 Contributions

We had a fantastic group effort on MultiQC at the 2017 BOSC CodeFest. Many thanks to those involved!

New Modules:

  • AfterQC - New module!
    • Added parsing of the AfterQC json file data, with a plot of filtered reads.
    • Work by @raonyguimaraes
  • bcl2fastq
    • bcl2fastq can be used to both demultiplex data and convert BCL files to FASTQ file formats for downstream analysis
    • New module parses JSON output from recent versions and summarises some key statistics from the demultiplexing process.
    • Work by @iimog (with a little help from @tbooth and @ewels)
  • leeHom
    • leeHom is a program for the Bayesian reconstruction of ancient DNA
  • VCFTools
    • Added initial support for VCFTools relatedness2
    • Added support for VCFTools TsTv-by-count TsTv-by-qual TsTv-summary
    • Module written by @mwhamgenomics

Module updates:

  • FastQ Screen
    • Gracefully handle missing data from very old FastQ Screen versions.
  • RNA-SeQC
    • Add new transcript-associated reads plot.
  • Picard
    • New submodule to handle output from TargetedPcrMetrics
  • Prokka
    • Added parsing of the # CRISPR arrays data from Prokka when available (@asetGem)
  • Qualimap
    • Some code refactoring to radically improve performance and run times, especially with high coverage datasets.
    • Fixed bug where Cumulative coverage genome fraction plot could be truncated.

New MultiQC Features:

  • New module help text
    • Lots of additional help text was written to make MultiQC report plots easier to interpret.
    • Updated modules:
      • Bowtie
      • Bowtie 2
      • Prokka
      • Qualimap
      • SnpEff
    • Elite team of help-writers:
  • New config option section_comments allows you to add custom comments above specific sections in the report
  • New --tags and --view_tags command line options
    • Modules can now be given tags (keywords) and filtered by those. So running --tags RNA will only run MultiQC modules related to RNA analysis.
    • Work by @Hammarn
  • Back-end configuration options to specify the order of table columns
    • Modules and user configs can set priorities for columns to customise where they are displayed
    • Work by @tbooth
  • Added framework for proper unit testing
    • Previous start on unit tests tidied up, new blank template and tests for the clean_sample_name functionality.
    • Added to Travis and Appveyor for continuous integration testing.
    • Work by @tbooth
  • Bug fixes and refactoring of report configuration saving / loading
    • Discovered and fixed a bug where a report config could only be loaded once
    • Work by @DennisSchwartz
  • Table column row headers (sample names) can now be numeric-only.
  • Improved sample name cleaning functionality
    • Added option regex_keep to clean filenames by keeping the matching part of a pattern
    • Work by @robinandeer
  • Handle error when invalid regexes are given in reports
    • Now have a nice toast error warning you and the invalid regexes are highlighted
    • Previously this just crashed the whole report without any warning
    • Work by @robinandeer
  • Command line option --dirs-depth now sets -d to True (so now works even if -d isn't also specified).
  • New config option config.data_dump_file to export as much data as possible to multiqc_data/multiqc_data.json
  • New code to send exported JSON data to a a web server
    • This is in preparation for the upcoming MegaQC project. Stay tuned!

Bug Fixes:

  • Specifying multiple config files with -c/--config now works as expected
    • Previously this would only read the last specified
  • Fixed table rendering bug that affected Chrome v60 and IE7-11
    • Table cell background bars weren't showing up. Updated CSS to get around this rendering error.
  • HTML ID cleanup now properly cleans strings so that they work with jQuery as expected.
  • Made bar graph sample highlighting work properly again
  • Config custom_logo paths can now be relative to the config file (or absolute as before)
  • Report doesn't keep annoyingly telling you that toolbox changes haven't been applied
    • Now uses more subtle toasts and only when you close the toolbox (not every click).
  • Switching report toolbox options to regex mode now enables the Apply button as it should.
  • Sorting table columns with certain suffixes (eg. 13X) no works properly (numerically)
  • Fixed minor bug in line plot data smoothing (now works with unsorted keys)

MultiQC v1.1 - 2017-07-18

New Modules:

  • BioBloom Tools
    • Create Bloom filters for a given reference and then to categorize sequences
  • Conpair
    • Concordance and contamination estimator for tumor–normal pairs
  • Disambiguate
    • Bargraph displaying the percentage of reads aligning to two different reference genomes.
  • Flexbar
    • Flexbar is a tool for flexible barcode and adapter removal.
  • HISAT2
    • New module for the HISAT2 aligner.
    • Made possible by updates to HISAT2 logging by @infphilo (requires --new-summary HISAT2 flag).
  • HOMER
    • Support for summary statistics from the findPeaks tool.
  • Jellyfish
    • Histograms to estimate library complexity and coverage from k-mer content.
    • Module written by @vezzi
  • MACS2
    • Summary of redundant rate from MACS2 peak calling.
  • QoRTs
    • QoRTs is toolkit for analysis, QC and data management of RNA-Seq datasets.
  • THetA2
    • THeTA2 (Tumor Heterogeneity Analysis) estimates tumour purity and clonal / subclonal copy number.

Module updates:

  • BCFtools
    • Option to collapse complementary changes in substitutions plot, useful for non-strand specific experiments (thanks to @vladsaveliev)
  • Bismark
    • M-Bias plots no longer show read 2 for single-end data.
  • Custom Content
    • New option to print raw HTML content to the report.
  • FastQ Screen
    • Fixed edge-case bug where many-sample plot broke if total number of reads was less than the subsample number.
    • Fixed incorrect logic of config option fastqscreen_simpleplot (thanks to @daler)
    • Organisms now alphabetically sorted in fancy plot so that order is nonrandom (thanks to @daler)
    • Fixed bug where %No Hits was missed in logs from recent versions of FastQ Screen.
  • HTSeq Counts
    • Fixed but so that module still works when --additional-attr is specified in v0.8 HTSeq above (thanks to @nalcala)
  • Picard
    • CollectInsertSize: Fixed bug that could make the General Statistics Median Insert Size value incorrect.
    • Fixed error in sample name regex that left trailing ] characters and was generally broken (thanks to @jyh1 for spotting this)
  • Preseq
    • Improved plots display (thanks to @vladsaveliev)
  • Qualimap
    • Only calculate bases over target coverage for values in General Statistics. Should give a speed increase for very high coverage datasets.
  • QUAST
    • Module is now compatible with runs from MetaQUAST (thanks to @vladsaveliev)
  • RSeQC
    • Changed default order of sections
    • Added config option to reorder and hide module report sections

New MultiQC features:

  • If a report already exists, execution is no longer halted.
    • _1 is appended to the filename, iterating if this also exists.
    • -f/--force still overwrites existing reports as before
    • Feature written by @Hammarn
  • New ability to run modules multiple times in a single report
    • Each run can be given different configuration options, including filters for input files
    • For example, have FastQC after trimming as well as FastQC before trimming.
    • See the relevant documentation for more instructions.
  • New option to customise the order of report sections
    • This is in addition / alternative to changing the order of module execution
    • Allows one module to have sections in multiple places (eg. Custom Content)
  • Tables have new column options floor, ceiling and minRange.
  • Reports show warning if JavaScript is disabled
  • Config option custom_logo now works with file paths relative to config file directory and cwd.

Bug Fixes:

  • Table headers now sort columns again after scrolling the table
  • Fixed buggy table header tooltips
  • Base clean_s_name function now strips excess whitespace.
  • Line graphs don't smooth lines if not needed (number of points < maximum number allowed)
  • PDF output now respects custom output directory.

MultiQC v1.0 - 2017-05-17

Version 1.0! This release has been a long time coming and brings with it some fairly major improvements in speed, report filesize and report performance. There's also a bunch of new modules, more options, features and a whole lot of bug fixes.

The version number is being bumped up to 1.0 for a couple of reasons:

  1. MultiQC is now (hopefully) relatively stable. A number of facilities and users are now using it in a production setting and it's published. It feels like it probably deserves v1 status now somehow.
  2. This update brings some fairly major changes which will break backwards compatibility for plugins. As such, semantic versioning suggests a change in major version number.

Breaking Changes

For most people, you shouldn't have any problems upgrading. There are two scenarios where you may need to make changes with this update:

1. You have custom file search patterns

Search patterns have been flattened and may no longer have arbitrary depth. For example, you may need to change the following:

fastqc:
    data:
        fn: 'fastqc_data.txt'
    zip:
        fn: '*_fastqc.zip'

to this:

fastqc/data:
    fn: 'fastqc_data.txt'
fastqc/zip:
    fn: '*_fastqc.zip'

See the documentation for instructions on how to write the new file search syntax.

See search_patterns.yaml for the new module search keys and more examples.

2. You have custom plugins / modules / external code

To see what changes need to applied to your custom plugin code, please see the MultiQC docs.

New Modules:

  • Adapter Removal
    • AdapterRemoval v2 - rapid adapter trimming, identification, and read merging
  • BUSCO
    • New module for the BUSCO v2 tool, used for assessing genome assembly and annotation completeness.
  • Cluster Flow
    • Cluster Flow is a workflow tool for bioinformatics pipelines. The new module parses executed tool commands.
  • RNA-SeQC
    • New module to parse output from RNA-SeQC, a java program which computes a series of quality control metrics for RNA-seq data.
  • goleft indexcov
    • goleft indexcov uses the PED and ROC data files to create diagnostic plots of coverage per sample, helping to identify sample gender and coverage issues.
    • Thanks to @chapmanb and @brentp
  • SortMeRNA
    • New module for SortMeRNA, commonly used for removing rRNA contamination from datasets.
    • Written by @bschiffthaler

Module updates:

  • Bcftools
    • Fixed bug with display of indels when only one sample
  • Cutadapt
    • Now takes the filename if the sample name is - (stdin). Thanks to @tdido
  • FastQC
    • Data for the Sequence content plot can now be downloaded from reports as a JSON file.
  • FastQ Screen
    • Rewritten plotting method for high sample numbers plot (~ > 20 samples)
    • Now shows counts for single-species hits and bins all multi-species hits
    • Allows plot to show proper percentage view for each sample, much easier to interpret.
  • HTSeq
    • Fix bug where header lines caused module to crash
  • Picard
    • New RrbsSummaryMetrics Submodule!
    • New WgsMetrics Submodule!
    • CollectGcBiasMetrics module now prints summary statistics to multiqc_data if found. Thanks to @ahvigil
  • Preseq
    • Now trims the x axis to the point that meets 90% of min(unique molecules). Hopefully prevents ridiculous x axes without sacrificing too much useful information.
    • Allows to show estimated depth of coverage instead of less informative molecule counts (see details).
    • Plots dots with externally calculated real read counts (see details).
  • Qualimap
    • RNASeq Transcript Profile now has correct axis units. Thanks to @roryk
    • BamQC module now doesn't crash if reports don't have genome gc distributions
  • RSeQC
    • Fixed Python3 error in Junction Saturation code
    • Fixed JS error for Junction Saturation that made the single-sample combined plot only show All Junctions

Core MultiQC updates:

  • Change in module structure and import statements (see details).
  • Module file search has been rewritten (see above changes to configs)
    • Significant improvement in search speed (test dataset runs in approximately half the time)
    • More options for modules to find their logs, eg. filename and contents matching regexes (see the docs)
  • Report plot data is now compressed, significantly reducing report filesizes.
  • New --ignore-samples option to skip samples based on parsed sample name
    • Alternative to filtering by input filename, which doesn't always work
    • Also can use config vars sample_names_ignore (glob patterns) and sample_names_ignore_re (regex patterns).
  • New --sample-names command line option to give file with alternative sample names
    • Allows one-click batch renaming in reports
  • New --cl_config option to supply MultiQC config YAML directly on the command line.
  • New config option to change numeric multiplier in General Stats
    • For example, if reports have few reads, can show Thousands of Reads instead of Millions of Reads
    • Set config options read_count_multiplier, read_count_prefix and read_count_desc
  • Config options decimalPoint_format and thousandsSep_format now apply to tables as well as plots
    • By default, thosands will now be separated with a space and . used for decimal places.
  • Tables now have a maximum-height by default and scroll within this.
    • Speeds up report rendering in the web browser and makes report less stupidly long with lots of samples
    • Button beneath table toggles full length if you want a zoomed-out view
    • Refactored and removed previous code to make the table header "float"
    • Set config.collapse_tables to False to disable table maximum-heights
  • Bar graphs and heatmaps can now be zoomed in on
    • Interactive plots sometimes hide labels due to lack of space. These can now be zoomed in on to see specific samples in more detail.
  • Report plots now load sequentially instead of all at once
    • Prevents the browser from locking up when large reports load
  • Report plot and section HTML IDs are now sanitised and checked for duplicates
  • New template available (called sections) which has faster loading
    • Only shows results from one module at a time
    • Makes big reports load in the browser much more quickly, but requires more clicking
    • Try it out by specifying -t sections
  • Module sections tidied and refactored
    • New helper function self.add_section()
    • Sections hidden in nav if no title (no more need for the hacky self.intro += )
    • Content broken into description, help and plot, with automatic formatting
    • Empty module sections are now skipped in reports. No need to check if a plot function returns None!
    • Changes should be backwards-compatible
  • Report plot data export code refactored
    • Now doesn't export hidden samples (uses HighCharts export-csv plugin)
  • Handle error when git isn't installed on the system.
  • Refactored colouring of table cells
    • Was previously done in the browser using chroma.js
    • Now done at report generation time using the spectra package
    • Should helpfully speed up report rendering time in the web browser, especially for large reports
  • Docs updates (thanks to @varemo)
  • Previously hidden log file .multiqc.log renamed to multiqc.log in multiqc_data
  • Added option to load MultiQC config file from a path specified in the environment variable MULTIQC_CONFIG_PATH
  • New table configuration options
    • sortRows: False prevents table rows from being sorted alphabetically
    • col1_header allows the default first column header to be changed from "Sample Name"
  • Tables no longer show Configure Columns and Plot buttons if they only have a single column
  • Custom content updates
    • New custom_content/order config option to specify order of Custom Content sections
    • Tables now use the header for the first column instead of always having Sample Name
    • JSON + YAML tables now remember order of table columns
    • Many minor bugfixes
  • Line graphs and scatter graphs axis limits
    • If limits are specified, data exceeding this is no longer saved in report
    • Visually identical, but can make report file sizes considerable smaller in some cases
  • Creating multiple plots without a config dict now works (previously just gave grey boxes in report)
  • All changes are now tested on a Windows system, using AppVeyor
  • Fixed rare error where some reports could get empty General Statistics tables when no data present.
  • Fixed minor bug where config option force: true didn't work. Now you don't have to always specify -f!

MultiQC v0.9 - 2016-12-21

A major new feature is released in v0.9 - support for custom content. This means that MultiQC can now easily include output from custom scripts within reports without the need for a new module or plugin. For more information, please see the MultiQC documentation.

New Modules:

  • HTSeq
    • New module for the htseq-count tool, often used in RNA-seq analysis.
  • Prokka
    • Prokka is a software tool for the rapid annotation of prokaryotic genomes.
  • Slamdunk
    • Slamdunk is a software tool to analyze SLAMSeq data.
  • Peddy
    • Peddy calculates genotype :: pedigree correspondence checks, ancestry checks and sex checks using VCF files.

Module updates:

  • Cutadapt
    • Fixed bug in General Stats table number for old versions of cutadapt (pre v1.7)
    • Added support for really old cutadapt logs (eg. v.1.2)
  • FastQC
    • New plot showing total overrepresented sequence percentages.
    • New option to parse a file containing a theoretical GC curve to display in the background.
  • featureCounts
    • Added parsing checks and catch failures for when non-featureCounts files are picked up by accident
  • GATK
    • Fixed logger error in VariantEval module.
  • Picard
    • Fixed missing sample overwriting bug in RnaSeqMetrics
    • New feature to customise coverage shown from HsMetrics in General Statistics table see the docs for info).
    • Fixed compatibility problem with output from CollectMultipleMetrics for CollectAlignmentSummaryMetrics
  • Preseq
    • Module now recognises output from c_curve mode.
  • RSeQC
    • Made the gene body coverage plot show the percentage view by default
    • Made gene body coverage properly handle sample names
  • Samtools
    • New module to show duplicate stats from rmdup logs
    • Fixed a couple of niggles in the idxstats plot
  • SnpEff
    • Fixed swapped axis labels in the Variant Quality plot
  • STAR
    • Fixed crash when there are 0 unmapped reads.
    • Sample name now taken from the directory name if no file prefix found.
  • Qualimap BamQC
    • Add a line for pre-calculated reference genome GC content
    • Plot cumulative coverage for values above 50x, align with the coverage histogram.
    • New ability to customise coverage thresholds shown in General Statistics table (see the docs for info).

Core MultiQC updates:

  • Support for custom content (see top of release notes).
  • New ninja report tool: make scatter plots of any two table columns!
  • Plot data now saved in multiqc_data when 'flat' image plots are created
    • Allows you easily re-plot the data (eg. in Excel) for further downstream investigation
  • Added 'Apply' button to Highlight / Rename / Hide.
    • These tools can become slow with large reports. This means that you can enter several things without having to wait for the report to replot each change.
  • Report heatmaps can now be sorted by highlight
  • New config options decimalPoint_format and thousandsSep_format
    • Allows you to change the default 1 234.56 number formatting for plots.
  • New config option top_modules allows you to specify modules that should come at the top of the report
  • Fixed bar plot bug where missing categories could shift data between samples
  • Report title now printed in the side navigation
  • Missing plot IDs added for easier plot exporting
  • Stopped giving warnings about skipping directories (now a debug message)
  • Added warnings in report about missing functionality for flat plots (exporting and toolbox)
  • Export button has contextual text for images / data
  • Fixed a bug where user config files were loaded twice
  • Fixed bug where module order was random if --module or --exclude was used.
  • Refactored code so that the order of modules can be changed in the user config
  • Beefed up code + docs in scatter plots back end and multiple bar plots.
  • Fixed a few back end nasties for Tables
    • Shared-key columns are no longer forced to share colour schemes
    • Fixed bug in lambda modified values when format string breaks
    • Supplying just data with no header information now works as advertised
  • Improvements to back end code for bar plots
    • New tt_decimals and tt_suffix options for bar plots
    • Bar plots now support yCeiling, yFloor and yMinRange, as with line plots.
    • New option hide_zero_cats:False to force legends to be shown even when all data is 0
  • General Stats Showing x of y columns count is fixed on page load.
  • Big code whitespace cleanup

MultiQC v0.8 - 2016-09-26

New Modules:

  • GATK
    • Added support for VariantEval reports, only parsing a little of the information in there so far, but it's a start.
    • Module originally written by @robinandeer at the OBF Codefest, finished off by @ewels
  • Bcftools
  • QUAST
    • QUAST is a tool for assessing de novo assemblies against reference genomes.

Module updates:

  • Bismark now supports reports from bam2nuc, giving Cytosine coverage in General Stats.
  • Bowtie1
    • Updated to try to find bowtie command before log, handle multiple logs in one file. Same as bowtie2.
  • FastQC
    • Sample pass/warn/fail lists now display properly even with large numbers of samples
    • Sequence content heatmap display is better with many samples
  • Kallisto
    • Now supports logs from SE data.
  • Picard
    • BaseDistributionByCycle - new submodule! Written by @mlusignan
    • RnaSeqMetrics - new submodule! This one by @ewels ;)
    • AlignmentSummaryMetrics - another new submodule!
    • Fixed truncated files crash bug for Python 3 (#306)
  • Qualimap RNASeqQC
    • Fixed parsing bug affecting counts in Genomic Origin plot.
    • Module now works with European style thousand separators (1.234,56 instead of 1,234.56)
  • RSeQC
    • infer_experiment - new submodule! Written by @Hammarn
  • Samtools
    • stats submodule now has separate bar graph showing alignment scores
    • flagstat - new submodule! Written by @HLWiencko
    • idxstats - new submodule! This one by @ewels again

Core MultiQC updates:

  • New --export/-p option to generate static images plot in multiqc_plots (.png, .svg and .pdf)
    • Configurable with export_plots, plots_dir_name and export_plot_formats config options
    • --flat option no longer saves plots in multiqc_data/multiqc_plots
  • New --comment/-b flag to add a comment to the top of reports.
  • New --dirs-depth/-dd flag to specify how many directories to prepend with --dirs/-d
    • Specifying a postive number will take that many directories from the end of the path
    • A negative number will take directories from the start of the path.
  • Directory paths now appended before cleaning, so fn_clean_exts will now affect these names.
  • New custom_logo attributes to add your own logo to reports.
  • New report_header_info config option to add arbitrary information to the top of reports.
  • New --pdf option to create a PDF report
    • Depends on Pandoc being installed and is in a beta-stage currently.
    • Note that specifying this will make MultiQC use the simple template, giving a HTML report with much reduced functionality.
  • New fn_clean_sample_names config option to turn off sample name cleaning
    • This will print the full filename for samples. Less pretty reports and rows on the General Statistics table won't line up, but can prevent overwriting.
  • Table header defaults can now be set easily
  • General Statistics table now hidden if empty.
  • Some new defaults in the sample name cleaning
  • Updated the simple template.
    • Now has no toolbox or nav, no JavaScript and is better suited for printing / PDFs.
    • New config.simple_output config flag so code knows when we're trying to avoid JS.
  • Fixed some bugs with config settings (eg. template) being overwritten.
  • NFS log file deletion bug fixed by @brainstorm (#265)
  • Fixed bug in --ignore behaviour with directory names.
  • Fixed nasty bug in beeswarm dot plots where sample names were mixed up (#278)
  • Beeswarm header text is now more informative (sample count with more info on a tooltip)
  • Beeswarm plots now work when reports have > 1000 samples
  • Fixed some buggy behaviour in saving / loading report highlighting + renaming configs (#354)

Many thanks to those at the OpenBio Codefest 2016 who worked on MultiQC projects.

MultiQC v0.7 - 2016-07-04

Module updates:

  • Kallisto - new module!
  • Picard
    • Code refactored to make maintenance and additions easier.
    • Big update to HsMetrics parsing - more results shown in report, new plots (by @lpantano)
    • Updated InsertSizeMetrics to understand logs generated by CollectMultipleMetrics (#215)
    • Newlines in picard output. Fixed by @dakl
  • Samtools
    • Code refactored
    • Rewrote the samtools stats code to display more stats in report with a beeswarm plot.
  • Qualimap
    • Rewritten to use latest methods and fix bugs.
    • Added Percentage Aligned column to general stats for BamQC module.
    • Extra table thresholds added by @avilella (hidden by default)
  • General Statistics
    • Some tweaks to the display defaults (FastQC, Bismark, Qualimap, SnpEff)
    • Now possible to skip the General Statistics section of the report with --exclude general_stats
  • Cutadapt module updated to recognise logs from old versions of cutadapt (<= v1.6)
  • Trimmomatic
    • Now handles , decimal places in percentage values.
    • Can cope with line breaks in log files (see issue #212)
  • FastQC refactored
    • Now skips zip files if the sample name has already been found. Speeds up MultiQC execution.
    • Code cleaned up. Parsing and data-structures standardised.
    • New popovers on Pass / Warn / Fail status bars showing sample names. Fast highlighting and hiding.
    • New column in General Stats (hidden by default) showing percentage of FastQC modules that failed.
  • SnpEff
    • Search pattern now more generic, should match reports from others.
    • Counts by Effect plot removed (had hundreds of categories, was fairly unusable).
    • KeyError bug fixed.
  • Samblaster now gets sample name from ID instead of SM (@dakl)
  • Bowtie 2
    • Now parses overall alignment rate as intended.
    • Now depends on even less log contents to work with more inputs.
  • MethylQA now handles variable spacing in logs
  • featureCounts now splits columns on tabs instead of whitespace, can handle filenames with spaces

Core MultiQC updates:

  • Galaxy: MultiQC now available in Galax! Work by @devengineson / @yvanlebras / @cmonjeau
  • Heatmap: New plot type!
  • Scatter Plot: New plot type!
  • Download raw data behind plots in reports! Available in the Export toolbox.
    • Choose from tab-separated, comma-separated and the complete JSON.
  • Table columns can be hidden on page load (shown through Configure Columns)
    • Defaults are configurable using the table_columns_visible config option.
  • Beeswarm plot: Added missing rename / highlight / hiding functionality.
  • New -l / --file-list option: specify a file containing a list of files to search.
  • Updated HighCharts to v4.2.5. Added option to export to JPEG.
  • Can now cancel execution with a single ctrl+c rather than having to button mash
  • More granular control of skipping files during scan (filename, dirname, path matching)
    • Fixed --exclude so that it works with directories as well as files
  • New Clear button in toolbox to bulk remove highlighting / renaming / hiding filters.
  • Improved documentation about behaviour for large sample numbers.
  • Handle YAML parsing errors for the config file more gracefully
  • Removed empty columns from tables again
  • Fixed bug in changing module search patterns, reported by @lweasel
  • Added timeout parameter to version check to prevent hang on systems with long defaults
  • Fixed table display bug in Firefox
  • Fixed bug related to order in which config files are loaded
  • Fixed bug that broke the "Show only" toolbox feature with multiple names.
  • Numerous other small bugs.

MultiQC v0.6 - 2016-04-29

Module updates:

  • New Salmon module.
  • New Trimmomatic module.
  • New Bamtools stats module.
  • New beeswarm plot type. General Stats table replaced with this when many samples in report.
  • New RSeQC module: Actually a suite of 8 new modules supporting various outputs from RSeQC
  • Rewrote bowtie2 module: Now better at parsing logs and tries to scrape input from wrapper logs.
  • Made cutadapt show counts by default instead of obs/exp
  • Added percentage view to Picard insert size plot

Core MultiQC updates:

  • Dynamic plots now update their labels properly when changing datasets and to percentages
  • Config files now loaded from working directory if present
  • Started new docs describing how each module works
  • Refactored featureCounts module. Now handles summaries describing multiple samples.
  • Stopped using so many hidden files. .multiqc.log now called multiqc.log
  • New -c/--config command line option to specify a MultiQC configuration file
  • Can now load run-specific config files called multiqc_config.yaml in working directory
  • Large code refactoring - moved plotting code out of BaseModule and into new multiqc.plots submodules
  • Generalised code used to generate the General Stats table so that it can be used by modules
  • Removed interactive report tour, replaced with a link to a youtube tutorial
  • Made it possible to permanently hide the blue welcome message for all future reports
  • New option to smooth data for line plots. Avoids mega-huge plots. Applied to SnpEff, RSeQC, Picard.

Bugfixes:

  • Qualimap handles infinity symbol (thanks @chapmanb )
  • Made SnpEff less fussy about required fields for making plots
  • UTF-8 file paths handled properly in Py2.7+
  • Extending two config variables wasn't working. Now fixed.
  • Dragging the height bar of plots now works again.
  • Plots now properly change y axis limits and labels when changing datasets
  • Flat plots now have correct path in default_dev template

MultiQC v0.5 - 2016-03-29

Module updates:

Core MultiQC updates:

  • New "Flat" image plots, rendered at run time with MatPlotLib
    • By default, will use image plots if > 50 samples (set in config as plots_flat_numseries)
    • Means that very large numbers of samples can be viewed in reports. eg. single cell data.
    • Templates can now specify their own plotting functions
    • Use --flat and --interactive to override this behaviour
  • MultiQC added to bioconda (with help from @dakl)
  • New plugin hook: config_loaded
  • Plugins can now add new command line options (thanks to @robinandeer)
  • Changed default data directory name from multiqc_report_data to multiqc_data
  • Removed support for depreciated MultiQC_OSXApp
  • Updated logging so that a verbose multiqc_data/.multiqc.log file is always written
  • Now logs more stuff in verbose mode - command used, user configs and so on.
  • Added a call to multiqc.info to check for new versions. Disable with config no_version_check
  • Removed general stats manual row sorting.
  • Made filename matching use glob unix style filename match patterns
  • Everything (including the data directory) is now created in a temporary directory and moved when MultiQC is complete.
  • A handful of performance updates for large analysis directories

MultiQC v0.4 - 2016-02-16

  • New multiqc_sources.txt which identifies the paths used to collect all report data for each sample
  • Export parsed data as tab-delimited text, JSON or YAML using the new -k/--data-format command line option
  • Updated HighCharts from v4.2.2 to v4.2.3, fixes tooltip hover bug.
  • Nicer export button. Now tied to the export toolbox, hopefully more intuitive.
  • FastQC: Per base sequence content heatmap can now be clicked to show line graph for single sample
  • FastQC: No longer show adapter contamination datasets with <= 0.1% contamination.
  • Picard: Added support for CollectOxoGMetrics reports.
  • Changed command line option --name to --filename
  • --name also used for filename if --filename not specified.
  • Hide samples toolbox now has switch to show only matching samples
  • New regex help box with examples added to report
  • New button to copy general stats table to the clipboard
  • General Stats table 'floating' header now sorts properly when scrolling
  • Bugfix: MultiQC default_dev template now copies module assets properly
  • Bufgix: General Stats table floating header now resizes properly when page width changes

MultiQC v0.3.2 - 2016-02-08

  • All modules now load their log file search parameters from a config file, allowing you to overwrite them using your user config file
    • This is useful if your analysis pipeline renames program outputs
  • New Picard (sub)modules - Insert Size, GC Bias & HsMetrics
  • New Qualimap (sub)module - RNA-Seq QC
  • Made Picard MarkDups show percent by default instead of counts
  • Added M-Bias plot to Bismark
  • New option to stream report HTML to stdout
  • Files can now be specified as well as directories
  • New options to specify whether the parsed data directory should be created
    • command line flags: --data / --no-data
    • config option name: make_data_dir
  • Fixed bug with incorrect path to installation dir config YAML file
  • New toolbox drawer for bulk-exporting graph images
  • Report side navigation can now be hidden to maximise horizontal space
  • Mobile styling improved for narrow screen
  • More vibrant colours in the general stats table
  • General stats table numbers now left aligned
  • Settings now saved and loaded to named localstorage locations
    • Simplified interface - no longer global / single report saving
    • Removed static file config. Solves JS error, no-one was doing this since we have standalone reports anyway.
  • Added support for Python 3.5
  • Fixed bug with module specific CSS / JS includes in some templates
  • Made the 'ignore files' config use unix style file pattern matching
  • Fixed some bugs in the FastQ Screen module
  • Fixed some bugs in the FastQC module
  • Fixed occasional general stats table bug
  • Table sorting on sample names now works after renaming
  • Bismark module restructure
    • Each report type now handled independently (alignment / dedup / meth extraction)
    • M-Bias plot now shows R1 and R2
  • FastQC GC content plot now has option for counts or percentages
    • Allows comparison between samples with very different read counts
  • Bugfix for reports javascript
    • Caused by updated to remotely loaded HighCharts export script
    • Export script now bundled with multiqc, so does not depend on internet connection
    • Other JS errors fixed in this work
  • Bugfix for older FastQC reports - handle old style sequence dup data
  • Bugfix for varying Tophat alignment report formats
  • Bugfix for Qualimap RNA Seq reports with paired end data

MultiQC v0.3.1 - 2015-11-04

  • Hotfix patch to fix broken FastQC module (wasn't finding .zip files properly)
  • General Stats table colours now flat. Should improve browser speed.
  • Empty rows now hidden if appear due to column removal in general stats
  • FastQC Kmer plot removed until we have something better to show.

MultiQC v0.3 - 2015-11-04

  • Lots of lovely new documentation!
  • Child templates - easily customise specific parts of the default report template
  • Plugin hooks - allow other tools to execute custom code during MultiQC execution
  • New Preseq module
  • New design for general statistics table (snazzy new background bars)
  • Further development of toolbox
    • New button to clear all filters
    • Warnings when samples are hidden, plus empty plots and table cols are hidden
    • Active toolbar tab buttons are highlighted
  • Lots of refactoring by @moonso to please the Pythonic gods
    • Switched to click instead of argparse to handle command line arguments
    • Code generally conforms to best practices better now.
  • Now able to supply multiple directories to search for reports
  • Logging output improved (now controlled by -q and -v for quiet and verbose)
  • More HTML output dealt with by the base module, less left to the modules
    • Module introduction text
    • General statistics table now much easier to add to (new helper functions)
  • Images, CSS and Javascript now included in HTML, meaning that there is a single report file to make sharing easier
  • More accessible scrolling in the report - styled scrollbars and 'to top' button.
  • Modules and templates now use setuptools entry points, facilitating plugins by other packages. Allows niche extensions whilst keeping the core codebase clean.
  • The general stats table now has a sticky header row when scrolling, thanks to some new javascript wizardry...
  • General stats columns can have a shared key which allows common colour schemes and data ranges. For instance, all columns describing a read count will now share their scale across modules.
  • General stats columns can be hidden and reordered with a new modal window.
  • Plotting code refactored, reports with many samples (>50 by default) don't automatically render to avoid freezing the browser.
  • Plots with highlighted and renamed samples now honour this when exporting to different file types.

MultiQC v0.2 - 2015-09-18

  • Code restructuring for nearly all modules. Common base module functions now handle many more functions (plots, config, file import)
    • See the contributing notes for instructions on how to use these new helpers to make your own module
  • New report toolbox - sample highlighting, renaming, hiding
    • Config is autosaved by default, can also export to a file for sharing
    • Interactive tour to help users find their way around
  • New Tophat, Bowtie 2 and QualiMap modules
    • Thanks to @guillermo-carrasco for the QualiMap module
  • Bowtie module now works
  • New command line parameter -d prefixes sample names with the directory that they were found in. Allows duplicate filenames without being overwritten.
  • Introduction walkthrough helps show what can be done in the report
  • Now compatible with both Python 2 and Python 3
  • Software version number now printed on command line properly, and in reports.
  • Bugfix: FastQC doesn't break when only one report found
  • Bugfix: FastQC seq content heatmap highlighting
  • Many, many small bugfixes

MultiQC v0.1 - 2015-09-01

  • The first public release of MultiQC, after a month of development. Basic structure in place and modules for FastQC, FastQ Screen, Cutadapt, Bismark, STAR, Bowtie, Subread featureCounts and Picard MarkDuplicates. Approaching stability, though still under fairly heavy development.