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error occured whe running Epinano_Variants.py #149

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Seongmin-Jang-1165 opened this issue Oct 15, 2024 · 2 comments
Open

error occured whe running Epinano_Variants.py #149

Seongmin-Jang-1165 opened this issue Oct 15, 2024 · 2 comments

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@Seongmin-Jang-1165
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hello

i ran Epinano for analyze m6a modification with oure Direct RNA seq data

but when i started, it did not work and the error message is below:

Please offer correctly path to sam2tsv.jar

how can i fix this error?

i ran Epinano under Bioconda Environment
@enovoa
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enovoa commented Oct 15, 2024 •
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Please check the version of Epinano you are using, as stated in the README and the description of Epinano_variants.py, Epinano v1.2.4 does not rely on sam2tsv.jar any more

@enovoa enovoa mentioned this issue Oct 16, 2024
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@Seongmin-Jang-1165
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@enovoa
thanks for advice!

does basecalled data for Epinano must be generated using Guppy3.1.5? my data is POD5 format and i tried to run Epinano with DORADO basecaller

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@enovoa @Seongmin-Jang-1165