From 4e1e8dc80a46a84de2a08b151cbe4181f8231e83 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 21 Jul 2023 15:37:54 +0200 Subject: [PATCH 001/387] bumpversion on dev --- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 8388320b..73191ec7 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -3,9 +3,9 @@ custom_logo_url: https://github.com/nf-core/raredisease/ custom_logo_title: "nf-core/raredisease" report_comment: > - This report has been generated by the nf-core/raredisease + This report has been generated by the nf-core/raredisease analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-raredisease-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index 9faa8055..185940df 100644 --- a/nextflow.config +++ b/nextflow.config @@ -271,7 +271,7 @@ manifest { description = """call and score variants from WGS/WES of rare disease patients""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.1.0' + version = '1.2.0dev' doi = '' } From d0065c8ccabca8597d12e19fc3d573095be68a95 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 21 Jul 2023 15:42:03 +0200 Subject: [PATCH 002/387] update changelog --- CHANGELOG.md | 6 ++++++ 1 file changed, 6 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 9c92d787..6a5f5f94 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,12 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). +## v1.2.0 - Genie [xxxx-xx-xx] + +### `Added` + +### `Fixed` + ## v1.1.0 - Abu [2023-07-21] ### `Added` From b7a795cb89f57508dc3f99e006a48c9b348375d6 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 21 Jul 2023 15:42:03 +0200 Subject: [PATCH 003/387] update changelog --- CHANGELOG.md | 6 ++++++ 1 file changed, 6 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 9c92d787..6a5f5f94 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,12 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). +## v1.2.0 - Genie [xxxx-xx-xx] + +### `Added` + +### `Fixed` + ## v1.1.0 - Abu [2023-07-21] ### `Added` From a917003b921a82821c2add3918b27ee5181f8337 Mon Sep 17 00:00:00 2001 From: Anders Sune Pedersen Date: Sun, 23 Jul 2023 15:27:43 +0200 Subject: [PATCH 004/387] Updating tiddit to v3.6.1 --- modules.json | 4 ++-- modules/nf-core/tiddit/cov/main.nf | 6 +++--- modules/nf-core/tiddit/sv/main.nf | 6 +++--- 3 files changed, 8 insertions(+), 8 deletions(-) diff --git a/modules.json b/modules.json index da9ac319..ef81841f 100644 --- a/modules.json +++ b/modules.json @@ -367,12 +367,12 @@ }, "tiddit/cov": { "branch": "master", - "git_sha": "0460d316170f75f323111b4a2c0a2989f0c32013", + "git_sha": "1c90a501d102b800c27697f5ef39a6e217ab1915", "installed_by": ["modules"] }, "tiddit/sv": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "1c90a501d102b800c27697f5ef39a6e217ab1915", "installed_by": ["modules"] }, "ucsc/wigtobigwig": { diff --git a/modules/nf-core/tiddit/cov/main.nf b/modules/nf-core/tiddit/cov/main.nf index 647f35a2..f4b1d675 100644 --- a/modules/nf-core/tiddit/cov/main.nf +++ b/modules/nf-core/tiddit/cov/main.nf @@ -2,10 +2,10 @@ process TIDDIT_COV { tag "$meta.id" label 'process_low' - conda "bioconda::tiddit=3.3.2" + conda "bioconda::tiddit=3.6.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/tiddit:3.3.2--py310hc2b7f4b_0' : - 'biocontainers/tiddit:3.3.2--py310hc2b7f4b_0' }" + 'https://depot.galaxyproject.org/singularity/tiddit:3.6.1--py38h24c8ff8_0' : + 'biocontainers/tiddit:3.6.1--py38h24c8ff8_0' }" input: tuple val(meta), path(input) diff --git a/modules/nf-core/tiddit/sv/main.nf b/modules/nf-core/tiddit/sv/main.nf index 1ebc8565..67a0670d 100644 --- a/modules/nf-core/tiddit/sv/main.nf +++ b/modules/nf-core/tiddit/sv/main.nf @@ -2,10 +2,10 @@ process TIDDIT_SV { tag "$meta.id" label 'process_medium' - conda "bioconda::tiddit=3.3.2" + conda "bioconda::tiddit=3.6.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/tiddit:3.3.2--py310hc2b7f4b_0' : - 'biocontainers/tiddit:3.3.2--py310hc2b7f4b_0' }" + 'https://depot.galaxyproject.org/singularity/tiddit:3.6.1--py38h24c8ff8_0' : + 'biocontainers/tiddit:3.6.1--py38h24c8ff8_0' }" input: tuple val(meta), path(input), path(input_index) From 492f69660842f429d00fc80577b716efea7b34bb Mon Sep 17 00:00:00 2001 From: Anders Sune Pedersen Date: Sun, 23 Jul 2023 15:48:24 +0200 Subject: [PATCH 005/387] Updating tiddit to v3.6.1 --- CHANGELOG.md | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 9c92d787..2d216e05 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -16,6 +16,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - UPD+Chromograph to identify and visualize UPD sites and regions in the chromosomes [#364](https://github.com/nf-core/raredisease/pull/364) and [#366](https://github.com/nf-core/raredisease/pull/366) - Added check for presence of case id for each sample in samplesheet [#357](https://github.com/nf-core/raredisease/pull/357) +### Changed + +- [#385](https://github.com/nf-core/sarek/pull/385) - Updating tiddit to v3.6.1 + ### Fixed - Avoiding publishing uncompressed VCF-file from `HMTNOTE_ANNOTATE`. (The corresponding compressed VCF-file still gets published.) [#368](https://github.com/nf-core/raredisease/pull/368) From 515c24b102bba19e2097dc11fddfe050952e5280 Mon Sep 17 00:00:00 2001 From: Anders Sune Pedersen Date: Mon, 24 Jul 2023 11:54:50 +0200 Subject: [PATCH 006/387] Adding section for v1.2.0dev in changelog --- CHANGELOG.md | 10 ++++++---- 1 file changed, 6 insertions(+), 4 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 2d216e05..51bc1ad6 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,12 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). +## v1.2.0dev + +### Changed + +- Tiddit updated to v3.6.1 [#385](https://github.com/nf-core/sarek/pull/385) + ## v1.1.0 - Abu [2023-07-21] ### `Added` @@ -16,10 +22,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - UPD+Chromograph to identify and visualize UPD sites and regions in the chromosomes [#364](https://github.com/nf-core/raredisease/pull/364) and [#366](https://github.com/nf-core/raredisease/pull/366) - Added check for presence of case id for each sample in samplesheet [#357](https://github.com/nf-core/raredisease/pull/357) -### Changed - -- [#385](https://github.com/nf-core/sarek/pull/385) - Updating tiddit to v3.6.1 - ### Fixed - Avoiding publishing uncompressed VCF-file from `HMTNOTE_ANNOTATE`. (The corresponding compressed VCF-file still gets published.) [#368](https://github.com/nf-core/raredisease/pull/368) From b270d2c398e6b1c0e1300512a12f84ff70c04278 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 24 Jul 2023 21:49:30 +0200 Subject: [PATCH 007/387] switch to validation --- assets/schema_input.json | 13 ++++ conf/modules/raredisease.config | 8 -- modules/local/samplesheet_check.nf | 31 -------- nextflow_schema.json | 8 +- pyproject.toml | 2 +- subworkflows/local/check_input.nf | 116 ----------------------------- tower.yml | 2 - workflows/raredisease.nf | 96 +++++++++++++++++++----- 8 files changed, 99 insertions(+), 177 deletions(-) delete mode 100644 modules/local/samplesheet_check.nf delete mode 100644 subworkflows/local/check_input.nf diff --git a/assets/schema_input.json b/assets/schema_input.json index 602ec301..165a1830 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -9,11 +9,19 @@ "properties": { "sample": { "type": "string", + "exists": true, + "meta": ["id"], "pattern": "^\\S+$", "errorMessage": "Sample name must be provided and cannot contain spaces" }, + "lane": { + "type": "number", + "meta": ["lane"] + }, "fastq_1": { "type": "string", + "format": "file-path", + "exists": true, "pattern": "^\\S+\\.f(ast)?q\\.gz$", "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" }, @@ -32,16 +40,19 @@ }, "sex": { "type": "string", + "meta": ["sex"], "pattern": "^\\S+$", "errorMessage": "Sex must be provided and cannot contain spaces" }, "phenotype": { "type": "string", + "meta": ["phenotype"], "pattern": "^\\S+$", "errorMessage": "Phenotype must be provided and cannot contain spaces" }, "paternal_id": { "errorMessage": "Paternal ID cannot contain spaces", + "meta": ["paternal"], "anyOf": [ { "type": "string", @@ -55,6 +66,7 @@ }, "maternal_id": { "errorMessage": "Maternal ID cannot contain spaces", + "meta": ["maternal"], "anyOf": [ { "type": "string", @@ -68,6 +80,7 @@ }, "case_id": { "type": "string", + "meta": ["case_id"], "pattern": "^\\S+$", "errorMessage": "Case name must be provided and cannot contain spaces" } diff --git a/conf/modules/raredisease.config b/conf/modules/raredisease.config index cf5dea7b..845d5a55 100644 --- a/conf/modules/raredisease.config +++ b/conf/modules/raredisease.config @@ -17,14 +17,6 @@ process { - withName: '.*CHECK_INPUT:SAMPLESHEET_CHECK' { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: 'CUSTOM_DUMPSOFTWAREVERSIONS' { publishDir = [ path: { "${params.outdir}/pipeline_info" }, diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index 4dc269ea..00000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -process SAMPLESHEET_CHECK { - tag "$samplesheet" - label 'process_single' - - conda "conda-forge::python=3.8.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" - - input: - path samplesheet - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // This script is bundled with the pipeline, in nf-core/raredisease/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/nextflow_schema.json b/nextflow_schema.json index 5367ada4..0a4039f9 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,12 +10,13 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "outdir"], + "required": ["outdir"], "properties": { "input": { "type": "string", "format": "file-path", "exists": true, + "schema": "assets/schema_input.json", "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", "description": "Path to comma-separated file containing information about the samples in the experiment.", @@ -621,18 +622,23 @@ "multiqc_config": { "type": "string", "format": "file-path", + "exists": true, "description": "Custom config file to supply to MultiQC.", "fa_icon": "fas fa-cog", "hidden": true }, "multiqc_logo": { "type": "string", + "format": "file-path", + "exists": true, "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", "fa_icon": "fas fa-image", "hidden": true }, "multiqc_methods_description": { "type": "string", + "format": "file-path", + "exists": true, "description": "Custom MultiQC yaml file containing HTML including a methods description.", "fa_icon": "fas fa-cog", "hidden": true diff --git a/pyproject.toml b/pyproject.toml index 0d62beb6..bc01239b 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,4 +1,4 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Black. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. [tool.black] line-length = 120 diff --git a/subworkflows/local/check_input.nf b/subworkflows/local/check_input.nf deleted file mode 100644 index 3c862df2..00000000 --- a/subworkflows/local/check_input.nf +++ /dev/null @@ -1,116 +0,0 @@ -// -// Check input samplesheet and get read, sample, and case channels -// - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' - -workflow CHECK_INPUT { - take: - ch_samplesheet // channel: [mandatory] [ path(csv) ] - - main: - SAMPLESHEET_CHECK ( ch_samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .set { sheet } - - case_info = sheet - .toList() - .map {create_case_channel(it)} - - - reads = sheet.map { row -> [[row.sample.split('_')[0]], row] } - .groupTuple() - .map { meta, rows -> - [rows, rows.size()] - } - .transpose() - .map { row, numLanes -> - create_fastq_channel(row + [num_lanes:numLanes]) - } - - samples = sheet.map { create_samples_channel(it) } - - emit: - case_info // channel: [ val(case_info) ] - reads // channel: [ val(meta), [ path(reads) ] ] - samples // channel: [ val(sample_id), val(sex), val(phenotype), val(paternal_id), val(maternal_id), val(case_id) ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ path(versions.yml) ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.case_id = row.case_id - meta.sex = row.sex - meta.id = row.sample - meta.maternal = row.maternal_id - meta.paternal = row.paternal_id - meta.phenotype = row.phenotype - meta.single_end = row.single_end.toBoolean() - meta.num_lanes = row.num_lanes - meta.read_group = "\'@RG\\tID:"+ row.fastq_1.split('/')[-1] + "\\tPL:ILLUMINA\\tSM:"+row.sample.split('_')[0]+"\'" - - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - error("ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}") - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - error("ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}") - } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta -} - -// Function to get a list of metadata (e.g. pedigree, case id) from the sample; [ meta ] -def create_samples_channel(LinkedHashMap row) { - def sample = [:] - sample.id = row.sample - sample.sex = row.sex - sample.phenotype = row.phenotype - sample.maternal = row.maternal_id - sample.paternal = row.paternal_id - sample.case_id = row.case_id - - return sample -} - -// Function to get a list of metadata (e.g. case id) for the case [ meta ] -def create_case_channel(List rows) { - def case_info = [:] - def probands = [] - def upd_children = [] - def father = "" - def mother = "" - - for (item in rows) { - if (item.phenotype == "2") { - probands.add(item.sample.split("_T")[0]) - } - if ( (item.paternal_id!="0") && (item.paternal_id!="") && (item.maternal_id!="0") && (item.maternal_id!="") ) { - upd_children.add(item.sample.split("_T")[0]) - } - if ( (item.paternal_id!="0") && (item.paternal_id!="") ) { - father = item.paternal_id - } - if ( (item.maternal_id!="0") && (item.maternal_id!="") ) { - mother = item.maternal_id - } - } - - case_info.father = father - case_info.mother = mother - case_info.probands = probands - case_info.upd_children = upd_children - case_info.id = rows[0].case_id - - return case_info -} - diff --git a/tower.yml b/tower.yml index 2d5717b0..2eb333f8 100644 --- a/tower.yml +++ b/tower.yml @@ -1,8 +1,6 @@ reports: multiqc_report.html: display: "MultiQC HTML report" - samplesheet.valid.csv: - display: "Auto-created samplesheet with collated metadata and FASTQ paths" "*.ped": display: "Auto-created pedigree file" mimeType: "text" diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index e5497222..2c0bec10 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -4,7 +4,7 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryLog; paramsSummaryMap; fromSamplesheet } from 'plugin/nf-validation' def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) def citation = '\n' + WorkflowMain.citation(workflow) + '\n' @@ -76,9 +76,9 @@ if (missingParamsCount>0) { */ ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) +ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config ) : Channel.empty() +ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo ) : Channel.empty() +ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description ) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -117,7 +117,6 @@ include { ANNOTATE_STRUCTURAL_VARIANTS } from '../subworkflows/local/an include { CALL_REPEAT_EXPANSIONS } from '../subworkflows/local/call_repeat_expansions' include { CALL_SNV } from '../subworkflows/local/call_snv' include { CALL_STRUCTURAL_VARIANTS } from '../subworkflows/local/call_structural_variants' -include { CHECK_INPUT } from '../subworkflows/local/check_input' include { GENS } from '../subworkflows/local/gens' include { PREPARE_REFERENCES } from '../subworkflows/local/prepare_references' include { QC_BAM } from '../subworkflows/local/qc_bam' @@ -140,10 +139,41 @@ workflow RAREDISEASE { ch_versions = Channel.empty() - // Initialize input channels + // Initialize read, sample, and case_info channels ch_input = Channel.fromPath(params.input) - CHECK_INPUT (ch_input) - ch_versions = ch_versions.mix(CHECK_INPUT.out.versions) + Channel.fromSamplesheet("input") + .tap { ch_original_input } + .map { meta, fastq1, fastq2 -> meta.id } + .reduce([:]) { counts, sample -> //get counts of each sample in the samplesheet - for groupTuple + counts[sample] = (counts[sample] ?: 0) + 1 + counts + } + .combine( ch_original_input ) + .map { counts, meta, fastq1, fastq2 -> + new_meta = meta + [num_lanes:counts[meta.id], + read_group:"\'@RG\\tID:"+ fastq1.toString().split('/')[-1] + "\\tPL:ILLUMINA\\tSM:"+meta.id+"\'"] + if (!fastq2) { + return [ new_meta + [ single_end:true ], [ fastq1 ] ] + } else { + return [ new_meta + [ single_end:false ], [ fastq1, fastq2 ] ] + } + } + .tap{ ch_input_counts } + .map { meta, fastqs -> fastqs } + .reduce([:]) { counts, fastqs -> //get line number for each row to construct unique sample ids + counts[fastqs] = counts.size() + 1 + return counts + } + .combine( ch_input_counts ) + .map { lineno, meta, fastqs -> //append line number to sampleid + new_meta = meta + [id:meta.id+"_T"+lineno[fastqs]] + return [ new_meta, fastqs ] + } + .set { ch_reads } + + ch_samples = ch_reads.map { meta, fastqs -> meta} + ch_pedfile = ch_samples.toList().map { makePed(it) } + ch_case_info = ch_samples.toList().map { create_case_channel(it) } // Initialize file channels for PREPARE_REFERENCES subworkflow ch_genome_fasta = Channel.fromPath(params.fasta).map { it -> [[id:it[0].simpleName], it] }.collect() @@ -239,8 +269,6 @@ workflow RAREDISEASE { : Channel.value([]) ch_versions = ch_versions.mix(ch_references.versions) - // Generate pedigree file - ch_pedfile = CHECK_INPUT.out.samples.toList().map { makePed(it) } // SV caller priority if (params.skip_cnv_calling) { @@ -250,7 +278,7 @@ workflow RAREDISEASE { } // Input QC - FASTQC (CHECK_INPUT.out.reads) + FASTQC (ch_reads) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) // CREATE CHROMOSOME BED AND INTERVALS @@ -265,7 +293,7 @@ workflow RAREDISEASE { // ALIGNING READS, FETCH STATS, AND MERGE. ALIGN ( - CHECK_INPUT.out.reads, + ch_reads, ch_genome_fasta, ch_genome_fai, ch_genome_bwaindex, @@ -296,7 +324,7 @@ workflow RAREDISEASE { CALL_REPEAT_EXPANSIONS ( ch_mapped.bam_bai, ch_variant_catalog, - CHECK_INPUT.out.case_info, + ch_case_info, ch_genome_fasta, ch_genome_fai ) @@ -313,7 +341,7 @@ workflow RAREDISEASE { .toList() .set { ch_bai_list } - CHECK_INPUT.out.case_info + ch_case_info .combine(ch_bam_list) .combine(ch_bai_list) .set { ch_bams_bais } @@ -332,7 +360,7 @@ workflow RAREDISEASE { ch_dbsnp_tbi, ch_call_interval, ch_ml_model, - CHECK_INPUT.out.case_info + ch_case_info ) ch_versions = ch_versions.mix(CALL_SNV.out.versions) @@ -343,7 +371,7 @@ workflow RAREDISEASE { ch_genome_bwaindex, ch_genome_fasta, ch_genome_fai, - CHECK_INPUT.out.case_info, + ch_case_info, ch_target_bed, ch_genome_dictionary, ch_svcaller_priority, @@ -370,7 +398,7 @@ workflow RAREDISEASE { file(params.gens_interval_list), file(params.gens_pon), file(params.gens_gnomad_pos), - CHECK_INPUT.out.case_info, + ch_case_info, ch_genome_dictionary ) ch_versions = ch_versions.mix(GENS.out.versions) @@ -433,7 +461,7 @@ workflow RAREDISEASE { params.genome, params.vep_cache_version, ch_vep_cache, - CHECK_INPUT.out.case_info + ch_case_info ) ch_versions = ch_versions.mix(ANALYSE_MT.out.versions) @@ -598,6 +626,38 @@ def makePed(samples) { return outfile } +// Function to get a list of metadata (e.g. case id) for the case [ meta ] +def create_case_channel(List rows) { + def case_info = [:] + def probands = [] + def upd_children = [] + def father = "" + def mother = "" + + for (item in rows) { + if (item.phenotype == "2") { + probands.add(item.id.split("_T")[0]) + } + if ( (item.paternal!="0") && (item.paternal!="") && (item.maternal!="0") && (item.maternal!="") ) { + upd_children.add(item.id.split("_T")[0]) + } + if ( (item.paternal!="0") && (item.paternal!="") ) { + father = item.paternal + } + if ( (item.maternal!="0") && (item.maternal!="") ) { + mother = item.maternal + } + } + + case_info.father = father + case_info.mother = mother + case_info.probands = probands + case_info.upd_children = upd_children + case_info.id = rows[0].case_id + + return case_info +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END From e55102ad929a3ff628fe816a6ddf1d6b84ad7f32 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 24 Jul 2023 21:57:32 +0200 Subject: [PATCH 008/387] changelog update --- CHANGELOG.md | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 0d4b086b..8d000c78 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -7,9 +7,11 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Added` +- Use `nf-validation` plugin for parameter and samplesheet validation [#386](https://github.com/nf-core/raredisease/pull/386) + ### `Changed` -- Tiddit updated to v3.6.1 [#385](https://github.com/nf-core/sarek/pull/385) +- Tiddit updated to v3.6.1 [#385](https://github.com/nf-core/raredisease/pull/385) ### `Fixed` From 562d97bdcb75cb2da0be9577ea8a5e7ac680023f Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 24 Jul 2023 22:00:37 +0200 Subject: [PATCH 009/387] update lint --- .nf-core.yml | 1 + 1 file changed, 1 insertion(+) diff --git a/.nf-core.yml b/.nf-core.yml index 321436fe..93aadae1 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -5,4 +5,5 @@ lint: - .github/PULL_REQUEST_TEMPLATE.md - assets/multiqc_config.yaml - .github/workflows/linting.yml + - pyproject.toml repository_type: pipeline From f8a7ee8d5c1accf1ffa0f9afac7a13f0291089a2 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Marius=20Bj=C3=B8rnstad?= Date: Wed, 26 Jul 2023 10:44:37 +0200 Subject: [PATCH 010/387] Add documentation about running offline --- docs/usage.md | 30 ++++++++++++++++++++++++++++++ 1 file changed, 30 insertions(+) diff --git a/docs/usage.md b/docs/usage.md index 5b7f7aa6..96fa08a4 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -393,3 +393,33 @@ We recommend adding the following line to your environment to limit this (typica ```bash NXF_OPTS='-Xms1g -Xmx4g' ``` + +### Running the pipeline without Internet access + +The pipeline and container images can be downloaded using [nf-core tools](https://nf-co.re/docs/usage/offline). +Here is an example command to download pipeline version 1.1.0 with singularity images: + +``` +nf-core download \ + --container singularity \ + --compress none \ + --revision 1.1.0 \ + raredisease +``` +For running offline, you of course have to make all the reference data available locally, and specify `--fasta`, etc., see [above](#reference-files-and-parameters). + +Contrary to the paragraph about Nextflow on the nf-core tools page, it is not possible to use the "-all" packaged version of Nextflow. The online version of nextflow is necessary to support the nextflow plugins used for this pipeline. Download instead the file called just `nextflow`. Nextflow will download its dependencies when it is run. Additionally, you need to download the nf-validation plugin explicitly: + +``` +./nextflow plugin install nf-validation +``` + +Now you can transfer the `nextflow` binary as well as its directory `$HOME/.nextflow` to the system without Internet access, and use it there. It is necessary to use an explicit version of `nf-validation` offline, or Nextflow will check for the most recent version online. Find the version of nf-validation you downloaded in `$HOME/.nextflow/plugins`, then specify this version for `nf-validation` in your configuration file: + +``` +plugins { + // Set the plugin version explicitly, otherwise nextflow will look for the newest version online. + id 'nf-validation@0.3.1' +} +``` +This should go in your Nexflow confgiguration file, specified with `-c ` when running the pipeline. From d6e7394f209ccbdb6f62da15990a860d98687860 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Marius=20Bj=C3=B8rnstad?= Date: Wed, 26 Jul 2023 12:20:29 +0200 Subject: [PATCH 011/387] Use the correct name for new container system --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index 96fa08a4..c6ca7078 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -401,7 +401,7 @@ Here is an example command to download pipeline version 1.1.0 with singularity i ``` nf-core download \ - --container singularity \ + --container-system singularity \ --compress none \ --revision 1.1.0 \ raredisease From 7bdd5d862a0a7c7409b91d3965e24deb8c824538 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Marius=20Bj=C3=B8rnstad?= Date: Wed, 26 Jul 2023 12:29:58 +0200 Subject: [PATCH 012/387] Correct typo --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index c6ca7078..e989218f 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -422,4 +422,4 @@ plugins { id 'nf-validation@0.3.1' } ``` -This should go in your Nexflow confgiguration file, specified with `-c ` when running the pipeline. +This should go in your Nextflow confgiguration file, specified with `-c ` when running the pipeline. From f3deb8d979d915007d20106bffd1845ae6d41ae7 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Marius=20Bj=C3=B8rnstad?= Date: Wed, 26 Jul 2023 12:35:33 +0200 Subject: [PATCH 013/387] Point to the documentation to download Nextflow --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index e989218f..95ccf5ac 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -408,7 +408,7 @@ nf-core download \ ``` For running offline, you of course have to make all the reference data available locally, and specify `--fasta`, etc., see [above](#reference-files-and-parameters). -Contrary to the paragraph about Nextflow on the nf-core tools page, it is not possible to use the "-all" packaged version of Nextflow. The online version of nextflow is necessary to support the nextflow plugins used for this pipeline. Download instead the file called just `nextflow`. Nextflow will download its dependencies when it is run. Additionally, you need to download the nf-validation plugin explicitly: +Contrary to the paragraph about [Nextflow](https://nf-co.re/docs/usage/offline#nextflow) on the page linked above, it is not possible to use the "-all" packaged version of Nextflow. The online version of nextflow is necessary to support the nextflow plugins used for this pipeline. Download instead the file called just `nextflow`. Nextflow will download its dependencies when it is run. Additionally, you need to download the nf-validation plugin explicitly: ``` ./nextflow plugin install nf-validation From eaa4ca53174e02185d2f467977be4e5c8c9e7a6a Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Marius=20Bj=C3=B8rnstad?= Date: Wed, 26 Jul 2023 13:35:10 +0200 Subject: [PATCH 014/387] Removed the example and rephrased for clarity --- docs/usage.md | 14 ++------------ 1 file changed, 2 insertions(+), 12 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 95ccf5ac..06ce8cd6 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -396,19 +396,9 @@ NXF_OPTS='-Xms1g -Xmx4g' ### Running the pipeline without Internet access -The pipeline and container images can be downloaded using [nf-core tools](https://nf-co.re/docs/usage/offline). -Here is an example command to download pipeline version 1.1.0 with singularity images: +The pipeline and container images can be downloaded using [nf-core tools](https://nf-co.re/docs/usage/offline). For running offline, you of course have to make all the reference data available locally, and specify `--fasta`, etc., see [above](#reference-files-and-parameters). -``` -nf-core download \ - --container-system singularity \ - --compress none \ - --revision 1.1.0 \ - raredisease -``` -For running offline, you of course have to make all the reference data available locally, and specify `--fasta`, etc., see [above](#reference-files-and-parameters). - -Contrary to the paragraph about [Nextflow](https://nf-co.re/docs/usage/offline#nextflow) on the page linked above, it is not possible to use the "-all" packaged version of Nextflow. The online version of nextflow is necessary to support the nextflow plugins used for this pipeline. Download instead the file called just `nextflow`. Nextflow will download its dependencies when it is run. Additionally, you need to download the nf-validation plugin explicitly: +Contrary to the paragraph about [Nextflow](https://nf-co.re/docs/usage/offline#nextflow) on the page linked above, it is not possible to use the "-all" packaged version of Nextflow for this pipeline. The online version of Nextflow is necessary to support the necessary nextflow plugins. Download instead the file called just `nextflow`. Nextflow will download its dependencies when it is run. Additionally, you need to download the nf-validation plugin explicitly: ``` ./nextflow plugin install nf-validation From 6304e718d3c5d9641d6db0cdb31c3c1d9e4ab4cc Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Marius=20Bj=C3=B8rnstad?= Date: Wed, 26 Jul 2023 13:58:00 +0200 Subject: [PATCH 015/387] Spelling configuration Co-authored-by: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> --- docs/usage.md | 1 + 1 file changed, 1 insertion(+) diff --git a/docs/usage.md b/docs/usage.md index 06ce8cd6..70790df4 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -412,4 +412,5 @@ plugins { id 'nf-validation@0.3.1' } ``` + This should go in your Nextflow confgiguration file, specified with `-c ` when running the pipeline. From fd521c5639f0eab7907cc8f6162aa8339285b18b Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 26 Jul 2023 15:17:32 +0200 Subject: [PATCH 016/387] version --- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index ebb33ec8..73191ec7 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -3,9 +3,9 @@ custom_logo_url: https://github.com/nf-core/raredisease/ custom_logo_title: "nf-core/raredisease" report_comment: > - This report has been generated by the nf-core/raredisease + This report has been generated by the nf-core/raredisease analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-raredisease-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index 6ff7e6ce..1b503202 100644 --- a/nextflow.config +++ b/nextflow.config @@ -271,7 +271,7 @@ manifest { description = """call and score variants from WGS/WES of rare disease patients""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.1.1' + version = '1.2.0dev' doi = '' } From de8b2cc3d88e0059f6afce22a42fcce72fc4d2f1 Mon Sep 17 00:00:00 2001 From: Adam Talbot Date: Thu, 27 Jul 2023 11:10:53 +0100 Subject: [PATCH 017/387] Update GATK4 container to correct URI --- CHANGELOG.md | 2 ++ modules.json | 4 ++-- modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf | 2 +- modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf | 2 +- 4 files changed, 6 insertions(+), 4 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index de47d466..7399915b 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -15,6 +15,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Fixed` +- Invalid GATK4 container which caused incorrect singularity downloads with nf-core download [nf-core/modules #3668](https://github.com/nf-core/modules/issues/3668) + ## v1.1.1 - Abu (Patch) [2023-07-26] ### `Fixed` diff --git a/modules.json b/modules.json index ef81841f..38548e9b 100644 --- a/modules.json +++ b/modules.json @@ -117,7 +117,7 @@ }, "gatk4/determinegermlinecontigploidy": { "branch": "master", - "git_sha": "d25bf48327e86a7f737047a57ec264b90e22ce3d", + "git_sha": "c6257c167fb88d18f3c2797e1af331d28f7b8ae7", "installed_by": ["modules"] }, "gatk4/filtermutectcalls": { @@ -152,7 +152,7 @@ }, "gatk4/postprocessgermlinecnvcalls": { "branch": "master", - "git_sha": "39ca55cc30514169f8420162bafe4ecf673f4b9a", + "git_sha": "c6257c167fb88d18f3c2797e1af331d28f7b8ae7", "installed_by": ["modules"] }, "gatk4/preprocessintervals": { diff --git a/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf b/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf index 593c8968..407cc34c 100644 --- a/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf +++ b/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf @@ -4,7 +4,7 @@ process GATK4_DETERMINEGERMLINECONTIGPLOIDY { label 'process_single' //Conda is not supported at the moment: https://github.com/broadinstitute/gatk/issues/7811 - container "quay.io/nf-core/gatk:4.4.0.0" //Biocontainers is missing a package + container "nf-core/gatk:4.4.0.0" //Biocontainers is missing a package // Exit if running this module with -profile conda / -profile mamba if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { diff --git a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf index 8faf0121..d1a03fc1 100644 --- a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf +++ b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf @@ -3,7 +3,7 @@ process GATK4_POSTPROCESSGERMLINECNVCALLS { label 'process_single' //Conda is not supported at the moment: https://github.com/broadinstitute/gatk/issues/7811 - container "quay.io/nf-core/gatk:4.4.0.0" //Biocontainers is missing a package + container "nf-core/gatk:4.4.0.0" //Biocontainers is missing a package // Exit if running this module with -profile conda / -profile mamba if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { From a1c7ed2affc9797fe68d353db09a75296357b86c Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 31 Jul 2023 15:36:50 +0200 Subject: [PATCH 018/387] update markduplicates default prefix --- conf/modules/align.config | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/conf/modules/align.config b/conf/modules/align.config index 249dc81e..54dae858 100644 --- a/conf/modules/align.config +++ b/conf/modules/align.config @@ -19,8 +19,9 @@ process{ } withName: '.*ALIGN:SAMTOOLS_VIEW' { - ext.args = { '--output-fmt cram --write-index' } - ext.when = params.save_mapped_as_cram + ext.args = { '--output-fmt cram --write-index' } + ext.prefix = { "${meta.id}_sorted_md" } + ext.when = params.save_mapped_as_cram publishDir = [ path: { "${params.outdir}/alignment" }, mode: params.publish_dir_mode, From 7817473c5efa4ea841f8c89ac731a8e0d059b632 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 31 Jul 2023 15:49:27 +0200 Subject: [PATCH 019/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 7399915b..ab9e831b 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -16,6 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Fixed` - Invalid GATK4 container which caused incorrect singularity downloads with nf-core download [nf-core/modules #3668](https://github.com/nf-core/modules/issues/3668) +- Make the default cram prefix same as markduplicates prefix [#392](https://github.com/nf-core/raredisease/pull/392) ## v1.1.1 - Abu (Patch) [2023-07-26] From ac0e9a529de22d0401c3e965e31aff6129b84560 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 1 Aug 2023 16:14:43 +0200 Subject: [PATCH 020/387] sort SVs --- conf/modules/rank_variants.config | 14 +++++- modules.json | 10 +++++ modules/nf-core/bcftools/sort/main.nf | 60 ++++++++++++++++++++++++++ modules/nf-core/bcftools/sort/meta.yml | 43 ++++++++++++++++++ modules/nf-core/tabix/bgzip/main.nf | 54 +++++++++++++++++++++++ modules/nf-core/tabix/bgzip/meta.yml | 47 ++++++++++++++++++++ subworkflows/local/rank_variants.nf | 16 ++++--- 7 files changed, 237 insertions(+), 7 deletions(-) create mode 100644 modules/nf-core/bcftools/sort/main.nf create mode 100644 modules/nf-core/bcftools/sort/meta.yml create mode 100644 modules/nf-core/tabix/bgzip/main.nf create mode 100644 modules/nf-core/tabix/bgzip/meta.yml diff --git a/conf/modules/rank_variants.config b/conf/modules/rank_variants.config index 33a34733..1eee3385 100644 --- a/conf/modules/rank_variants.config +++ b/conf/modules/rank_variants.config @@ -37,14 +37,20 @@ process { ext.prefix = { "${meta.id}_ranked_sv" } } - withName: '.*RANK_VARIANTS_SV:TABIX_BGZIPTABIX' { + withName: '.*RANK_VARIANTS_SV:BCFTOOLS_SORT' { + ext.args = "--output-type z" ext.prefix = { "${meta.id}_ranked_sv" } + ext.when = true publishDir = [ path: { "${params.outdir}/rank_and_filter" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } + + withName: '.*RANK_VARIANTS_SV:TABIX_BGZIP' { + ext.when = false + } } // @@ -77,7 +83,11 @@ process { ext.prefix = { "${meta.id}_ranked_snv" } } - withName: '.*RANK_VARIANTS_SNV:TABIX_BGZIPTABIX' { + withName: '.*RANK_VARIANTS_SNV:BCFTOOLS_SORT' { + ext.when = false + } + + withName: '.*RANK_VARIANTS_SNV:TABIX_BGZIP' { ext.prefix = { "${meta.id}_ranked_snv" } publishDir = [ path: { "${params.outdir}/rank_and_filter" }, diff --git a/modules.json b/modules.json index 38548e9b..dbdc3daf 100644 --- a/modules.json +++ b/modules.json @@ -40,6 +40,11 @@ "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, + "bcftools/sort": { + "branch": "master", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": ["modules"] + }, "bcftools/view": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", @@ -355,6 +360,11 @@ "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, + "tabix/bgzip": { + "branch": "master", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": ["modules"] + }, "tabix/bgziptabix": { "branch": "master", "git_sha": "591b71642820933dcb3c954c934b397bd00d8e5e", diff --git a/modules/nf-core/bcftools/sort/main.nf b/modules/nf-core/bcftools/sort/main.nf new file mode 100644 index 00000000..ef41fd25 --- /dev/null +++ b/modules/nf-core/bcftools/sort/main.nf @@ -0,0 +1,60 @@ +process BCFTOOLS_SORT { + tag "$meta.id" + label 'process_medium' + + conda "bioconda::bcftools=1.17" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': + 'biocontainers/bcftools:1.17--haef29d1_0' }" + + input: + tuple val(meta), path(vcf) + + output: + tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}") , emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '--output-type z' + def prefix = task.ext.prefix ?: "${meta.id}" + def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf" + + """ + bcftools \\ + sort \\ + --output ${prefix}.${extension} \\ + $args \\ + $vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '--output-type z' + def prefix = task.ext.prefix ?: "${meta.id}" + + def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf" + + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/bcftools/sort/meta.yml b/modules/nf-core/bcftools/sort/meta.yml new file mode 100644 index 00000000..0c244a48 --- /dev/null +++ b/modules/nf-core/bcftools/sort/meta.yml @@ -0,0 +1,43 @@ +name: bcftools_sort +description: Sorts VCF files +keywords: + - sorting + - VCF + - variant calling +tools: + - sort: + description: Sort VCF files by coordinates. + homepage: http://samtools.github.io/bcftools/bcftools.html + documentation: http://www.htslib.org/doc/bcftools.html + tool_dev_url: https://github.com/samtools/bcftools + doi: "10.1093/bioinformatics/btp352" + licence: ["MIT"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: The VCF/BCF file to be sorted + pattern: "*.{vcf.gz,vcf,bcf}" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: Sorted VCF file + pattern: "*.{vcf.gz}" + +authors: + - "@Gwennid" diff --git a/modules/nf-core/tabix/bgzip/main.nf b/modules/nf-core/tabix/bgzip/main.nf new file mode 100644 index 00000000..8c47d9e2 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/main.nf @@ -0,0 +1,54 @@ +process TABIX_BGZIP { + tag "$meta.id" + label 'process_single' + + conda "bioconda::tabix=1.11" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : + 'biocontainers/tabix:1.11--hdfd78af_0' }" + + input: + tuple val(meta), path(input) + + output: + tuple val(meta), path("${output}") , emit: output + tuple val(meta), path("${output}.gzi"), emit: gzi, optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + in_bgzip = ["gz", "bgz", "bgzf"].contains(input.getExtension()) + extension = in_bgzip ? input.getBaseName().tokenize(".")[-1] : input.getExtension() + output = in_bgzip ? "${prefix}.${extension}" : "${prefix}.${extension}.gz" + command = in_bgzip ? '-d' : '' + // Name the index according to $prefix, unless a name has been requested + if ((args.matches("(^| )-i\\b") || args.matches("(^| )--index(\$| )")) && !args.matches("(^| )-I\\b") && !args.matches("(^| )--index-name\\b")) { + args = args + " -I ${output}.gzi" + } + """ + bgzip $command -c $args -@${task.cpus} $input > ${output} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + + stub: + prefix = task.ext.prefix ?: "${meta.id}" + in_bgzip = ["gz", "bgz", "bgzf"].contains(input.getExtension()) + output = in_bgzip ? input.getBaseName() : "${prefix}.${input.getExtension()}.gz" + + """ + touch ${output} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/tabix/bgzip/meta.yml b/modules/nf-core/tabix/bgzip/meta.yml new file mode 100644 index 00000000..c3ea2107 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/meta.yml @@ -0,0 +1,47 @@ +name: tabix_bgzip +description: Compresses/decompresses files +keywords: + - compress + - decompress + - bgzip + - tabix +tools: + - bgzip: + description: | + Bgzip compresses or decompresses files in a similar manner to, and compatible with, gzip. + homepage: https://www.htslib.org/doc/tabix.html + documentation: http://www.htslib.org/doc/bgzip.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input: + type: file + description: file to compress or to decompress +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - output: + type: file + description: Output compressed/decompressed file + pattern: "*." + - gzi: + type: file + description: Optional gzip index file for compressed inputs + pattern: "*.gzi" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" + - "@maxulysse" + - "@nvnieuwk" diff --git a/subworkflows/local/rank_variants.nf b/subworkflows/local/rank_variants.nf index e274de33..3049626f 100644 --- a/subworkflows/local/rank_variants.nf +++ b/subworkflows/local/rank_variants.nf @@ -6,7 +6,8 @@ include { GENMOD_ANNOTATE } from '../../modules/nf-core/genmod/annotate/main' include { GENMOD_MODELS } from '../../modules/nf-core/genmod/models/main' include { GENMOD_SCORE } from '../../modules/nf-core/genmod/score/main' include { GENMOD_COMPOUND } from '../../modules/nf-core/genmod/compound/main' -include { TABIX_BGZIPTABIX } from '../../modules/nf-core/tabix/bgziptabix/main' +include { BCFTOOLS_SORT } from '../../modules/nf-core/bcftools/sort/main' +include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip/main' workflow RANK_VARIANTS { @@ -27,15 +28,20 @@ workflow RANK_VARIANTS { GENMOD_COMPOUND(GENMOD_SCORE.out.vcf) - TABIX_BGZIPTABIX (GENMOD_COMPOUND.out.vcf) + BCFTOOLS_SORT(GENMOD_COMPOUND.out.vcf) // SV file needs to be sorted before indexing + + TABIX_BGZIP(GENMOD_COMPOUND.out.vcf) //run only for SNVs + + ch_vcf = TABIX_BGZIP.out.output.mix(BCFTOOLS_SORT.out.vcf) ch_versions = ch_versions.mix(GENMOD_ANNOTATE.out.versions) ch_versions = ch_versions.mix(GENMOD_MODELS.out.versions) ch_versions = ch_versions.mix(GENMOD_SCORE.out.versions) ch_versions = ch_versions.mix(GENMOD_COMPOUND.out.versions) - ch_versions = ch_versions.mix(TABIX_BGZIPTABIX.out.versions) + ch_versions = ch_versions.mix(BCFTOOLS_SORT.out.versions) + ch_versions = ch_versions.mix(TABIX_BGZIP.out.versions) emit: - vcf = TABIX_BGZIPTABIX.out.gz_tbi.map { meta, vcf, tbi -> return [ meta, vcf ] }.collect() // channel: [ val(meta), path(vcf) ] - versions = ch_versions // channel: [ path(versions.yml) ] + vcf = ch_vcf // channel: [ val(meta), path(vcf) ] + versions = ch_versions // channel: [ path(versions.yml) ] } From 525d80984a88f9579604c8535d2942b607904fff Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 1 Aug 2023 16:38:27 +0200 Subject: [PATCH 021/387] Update documentation --- docs/usage.md | 2 ++ nextflow_schema.json | 2 +- 2 files changed, 3 insertions(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index 5b7f7aa6..02ec085a 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -71,6 +71,8 @@ work # Directory containing the Nextflow working files Test profile runs the pipeline with a case containing three samples, but if you would like to test the pipeline with one sample, use `-profile test_one_sample,`. +> Note that the default cpu and memory configurations used in raredisease are written keeping the test profile (&dataset, which is tiny) in mind. You should override these values in configs to get it to work on larger datasets. Check the section `custom-configuration` below to know more about how to configure resources for your platform. + ### Updating the pipeline The above command downloads the pipeline from GitHub, caches it, and tests it on the test dataset. When you run the command again, it will fetch the pipeline from cache even if a more recent version of the pipeline is available. To make sure that you're running the latest version of the pipeline, update the cached version of the pipeline by including `-latest` in the command. diff --git a/nextflow_schema.json b/nextflow_schema.json index 0a4039f9..f5216d76 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -310,7 +310,7 @@ "exists": true, "format": "file-path", "description": "Path to variant catalog file", - "help_text": "Used with ExpansionHunter and if no catalogue is passed, then a default will be used.", + "help_text": "Should be Stranger's extended JSON as described at https://github.com/Clinical-Genomics/stranger/blob/master/stranger/resources/variant_catalog_grch37.json. This file is used by both ExpansionHunter and Stranger", "fa_icon": "fas fa-file" }, "vcfanno_resources": { From 8f664f8a723ee1734b885d21bf1a56f33831d456 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 1 Aug 2023 16:41:24 +0200 Subject: [PATCH 022/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index ab9e831b..f3b839b3 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -17,6 +17,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Invalid GATK4 container which caused incorrect singularity downloads with nf-core download [nf-core/modules #3668](https://github.com/nf-core/modules/issues/3668) - Make the default cram prefix same as markduplicates prefix [#392](https://github.com/nf-core/raredisease/pull/392) +- Sort ranked SV vcf before indexing with tabix [#393](https://github.com/nf-core/raredisease/pull/393) ## v1.1.1 - Abu (Patch) [2023-07-26] From 1b0773f81cdd6df6097c2602506a28890d7a3ed5 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 1 Aug 2023 18:22:17 +0200 Subject: [PATCH 023/387] update configs --- conf/modules/prepare_references.config | 10 +++++----- conf/modules/qc_bam.config | 2 +- 2 files changed, 6 insertions(+), 6 deletions(-) diff --git a/conf/modules/prepare_references.config b/conf/modules/prepare_references.config index 7d40508c..ab2b2ccf 100644 --- a/conf/modules/prepare_references.config +++ b/conf/modules/prepare_references.config @@ -86,20 +86,20 @@ process { } withName: '.*PREPARE_REFERENCES:TABIX_PT' { - ext.when = { params.target_bed && params.target_bed.endsWith(".gz") } + ext.when = { !params.target_bed.equals(null) && params.target_bed.endsWith(".gz") } } withName: '.*PREPARE_REFERENCES:TABIX_PBT' { - ext.when = { params.target_bed && !params.target_bed.endsWith(".gz") } + ext.when = { !params.target_bed.equals(null) && !params.target_bed.endsWith(".gz") } } withName: '.*PREPARE_REFERENCES:GATK_BILT' { - ext.when = { params.target_bed } + ext.when = { !params.target_bed.equals(null) } ext.prefix = { "${meta.id}_target" } } withName: '.*PREPARE_REFERENCES:GATK_ILT' { - ext.when = { params.target_bed } + ext.when = { !params.target_bed.equals(null) } ext.args = { "--PADDING ${params.bait_padding} -SUBDIVISION_MODE INTERVAL_SUBDIVISION --SCATTER_COUNT 2" } publishDir = [ enabled: false @@ -107,7 +107,7 @@ process { } withName: '.*PREPARE_REFERENCES:CAT_CAT_BAIT' { - ext.when = { params.target_bed } + ext.when = { !params.target_bed.equals("null") } ext.prefix = { "${meta.id}" } } diff --git a/conf/modules/qc_bam.config b/conf/modules/qc_bam.config index 080f988f..86780b72 100644 --- a/conf/modules/qc_bam.config +++ b/conf/modules/qc_bam.config @@ -29,7 +29,7 @@ process { } withName: '.*QC_BAM:PICARD_COLLECTHSMETRICS' { - ext.when = { params.target_bed } + ext.when = { !params.target_bed.equals(null) } ext.prefix = { "${meta.id}_hsmetrics" } } From 8a6c184bf9e465ccbc569bb87ababfff1dc19bd4 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 1 Aug 2023 18:33:09 +0200 Subject: [PATCH 024/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index ab9e831b..68eac568 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -17,6 +17,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Invalid GATK4 container which caused incorrect singularity downloads with nf-core download [nf-core/modules #3668](https://github.com/nf-core/modules/issues/3668) - Make the default cram prefix same as markduplicates prefix [#392](https://github.com/nf-core/raredisease/pull/392) +- Make target bed file optional for WGS mode (Issue [#375](https://github.com/nf-core/raredisease/issues/375)) [#395](https://github.com/nf-core/raredisease/pull/395) ## v1.1.1 - Abu (Patch) [2023-07-26] From 0b083eee887f88df4fd23c3283226fa842488cae Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 2 Aug 2023 00:53:10 +0200 Subject: [PATCH 025/387] fix typo --- conf/modules/prepare_references.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules/prepare_references.config b/conf/modules/prepare_references.config index ab2b2ccf..2532052d 100644 --- a/conf/modules/prepare_references.config +++ b/conf/modules/prepare_references.config @@ -107,7 +107,7 @@ process { } withName: '.*PREPARE_REFERENCES:CAT_CAT_BAIT' { - ext.when = { !params.target_bed.equals("null") } + ext.when = { !params.target_bed.equals(null) } ext.prefix = { "${meta.id}" } } From 783bcdac7504df66476f4e7f06b4479af41360c1 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 2 Aug 2023 17:18:49 +0200 Subject: [PATCH 026/387] update config --- conf/modules/qc_bam.config | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/conf/modules/qc_bam.config b/conf/modules/qc_bam.config index 080f988f..f0296709 100644 --- a/conf/modules/qc_bam.config +++ b/conf/modules/qc_bam.config @@ -52,22 +52,22 @@ process { } withName: '.*QC_BAM:PICARD_COLLECTWGSMETRICS' { - ext.when = params.aligner.equals("bwamem2") + ext.when = { params.analysis_type.equals("wgs") && params.aligner.equals("bwamem2") } ext.prefix = { "${meta.id}_wgsmetrics" } } withName: '.*QC_BAM:PICARD_COLLECTWGSMETRICS_Y' { - ext.when = params.aligner.equals("bwamem2") + ext.when = { params.analysis_type.equals("wgs") && params.aligner.equals("bwamem2") } ext.prefix = { "${meta.id}_wgsmetrics_y" } } withName: '.*QC_BAM:SENTIEON_WGSMETRICS' { - ext.when = params.aligner.equals("sentieon") + ext.when = { params.analysis_type.equals("wgs") && params.aligner.equals("sentieon") } ext.prefix = { "${meta.id}_wgsmetrics" } } withName: '.*QC_BAM:SENTIEON_WGSMETRICS_Y' { - ext.when = params.aligner.equals("sentieon") + ext.when = { params.analysis_type.equals("wgs") && params.aligner.equals("sentieon") } ext.prefix = { "${meta.id}_wgsmetrics_y" } } } From cb2dfafffc6adcbee260fa5e0d3020c633aef193 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 2 Aug 2023 17:20:20 +0200 Subject: [PATCH 027/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index ab9e831b..bb9bc7bf 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -17,6 +17,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Invalid GATK4 container which caused incorrect singularity downloads with nf-core download [nf-core/modules #3668](https://github.com/nf-core/modules/issues/3668) - Make the default cram prefix same as markduplicates prefix [#392](https://github.com/nf-core/raredisease/pull/392) +- Added constraints to block the pipeline from running CollectWgsMetrics on WES samples [#396](https://github.com/nf-core/raredisease/pull/396) ## v1.1.1 - Abu (Patch) [2023-07-26] From c7828d824c35c27eff043a805e5364ec1fa22a3e Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 7 Aug 2023 17:19:02 +0000 Subject: [PATCH 028/387] Updating module for sentieon bwamem --- modules.json | 5 +++ .../sentieon/bwaindex/main.nf} | 32 ++++++++++----- modules/nf-core/sentieon/bwaindex/meta.yml | 41 +++++++++++++++++++ subworkflows/local/prepare_references.nf | 5 +-- 4 files changed, 69 insertions(+), 14 deletions(-) rename modules/{local/sentieon/bwamemindex.nf => nf-core/sentieon/bwaindex/main.nf} (51%) create mode 100644 modules/nf-core/sentieon/bwaindex/meta.yml diff --git a/modules.json b/modules.json index da9ac319..e2c0bb2f 100644 --- a/modules.json +++ b/modules.json @@ -335,6 +335,11 @@ "git_sha": "3ffae3598260a99e8db3207dead9f73f87f90d1f", "installed_by": ["modules"] }, + "sentieon/bwaindex": { + "branch": "master", + "git_sha": "b9172e8c26a3db5009f7872654c44587e254f094", + "installed_by": ["modules"] + }, "smncopynumbercaller": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", diff --git a/modules/local/sentieon/bwamemindex.nf b/modules/nf-core/sentieon/bwaindex/main.nf similarity index 51% rename from modules/local/sentieon/bwamemindex.nf rename to modules/nf-core/sentieon/bwaindex/main.nf index 4b030975..0dde28d7 100644 --- a/modules/local/sentieon/bwamemindex.nf +++ b/modules/nf-core/sentieon/bwaindex/main.nf @@ -3,32 +3,32 @@ process SENTIEON_BWAINDEX { label 'process_high' label 'sentieon' - secret 'SENTIEON_LICENSE_BASE64' + container 'nf-core/sentieon:202112.06' input: tuple val(meta), path(fasta) output: - tuple val(meta), path("bwa/"), emit: index - path "versions.yml" , emit: versions + tuple val(meta), path(bwa), emit: index + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + } + def args = task.ext.args ?: '' def prefix = task.ext.prefix ? "bwa/${task.ext.prefix}" : "bwa/${fasta.baseName}" """ mkdir bwa - if [ \${SENTIEON_LICENSE_BASE64:-"unset"} != "unset" ]; then - echo "Initializing SENTIEON_LICENSE env variable" - source sentieon_init.sh SENTIEON_LICENSE_BASE64 - fi - - sentieon bwa index \\ + sentieon \\ + bwa index \\ $args \\ - -p $prefix \\ + -p bwa/${fasta.baseName} \\ $fasta cat <<-END_VERSIONS > versions.yml @@ -39,9 +39,19 @@ process SENTIEON_BWAINDEX { """ stub: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + } """ mkdir bwa + touch bwa/genome.amb + touch bwa/genome.ann + touch bwa/genome.bwt + touch bwa/genome.pac + touch bwa/genome.sa + cat <<-END_VERSIONS > versions.yml "${task.process}": sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") diff --git a/modules/nf-core/sentieon/bwaindex/meta.yml b/modules/nf-core/sentieon/bwaindex/meta.yml new file mode 100644 index 00000000..5d91e225 --- /dev/null +++ b/modules/nf-core/sentieon/bwaindex/meta.yml @@ -0,0 +1,41 @@ +name: sentieon_bwaindex +description: Create BWA index for reference genome +keywords: + - index + - fasta + - genome + - reference + - sentieon +tools: + - sentieon: + description: | + Sentieon® provides complete solutions for secondary DNA/RNA analysis for a variety of sequencing platforms, including short and long reads. + Our software improves upon BWA, STAR, Minimap2, GATK, HaplotypeCaller, Mutect, and Mutect2 based pipelines and is deployable on any generic-CPU-based computing system. + homepage: https://www.sentieon.com/ + documentation: https://www.sentieon.com/ +input: + - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - fasta: + type: file + description: Input genome fasta file +output: + - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - index: + type: file + description: BWA genome index files + pattern: "*.{amb,ann,bwt,pac,sa}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/local/prepare_references.nf b/subworkflows/local/prepare_references.nf index 45e20a24..9752b3f3 100644 --- a/subworkflows/local/prepare_references.nf +++ b/subworkflows/local/prepare_references.nf @@ -17,8 +17,8 @@ include { GET_CHROM_SIZES } from '../../modul include { SAMTOOLS_FAIDX as SAMTOOLS_EXTRACT_MT } from '../../modules/nf-core/samtools/faidx/main' include { SAMTOOLS_FAIDX as SAMTOOLS_FAIDX_GENOME } from '../../modules/nf-core/samtools/faidx/main' include { SAMTOOLS_FAIDX as SAMTOOLS_FAIDX_MT_SHIFT } from '../../modules/nf-core/samtools/faidx/main' -include { SENTIEON_BWAINDEX as SENTIEON_BWAINDEX_GENOME } from '../../modules/local/sentieon/bwamemindex' -include { SENTIEON_BWAINDEX as SENTIEON_BWAINDEX_MT_SHIFT } from '../../modules/local/sentieon/bwamemindex' +include { SENTIEON_BWAINDEX as SENTIEON_BWAINDEX_GENOME } from '../../modules/nf-core/sentieon/bwaindex/main' +include { SENTIEON_BWAINDEX as SENTIEON_BWAINDEX_MT_SHIFT } from '../../modules/nf-core/sentieon/bwaindex/main' include { TABIX_BGZIPTABIX as TABIX_PBT } from '../../modules/nf-core/tabix/bgziptabix/main' include { TABIX_TABIX as TABIX_DBSNP } from '../../modules/nf-core/tabix/tabix/main' include { TABIX_TABIX as TABIX_GNOMAD_AF } from '../../modules/nf-core/tabix/tabix/main' @@ -144,4 +144,3 @@ workflow PREPARE_REFERENCES { versions = ch_versions // channel: [ path(versions.yml) ] } - From fd75f359d543097653db851996e58aaecfd27833 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 7 Aug 2023 17:26:08 +0000 Subject: [PATCH 029/387] Setting output path using the prefix parameter --- modules/nf-core/sentieon/bwaindex/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/nf-core/sentieon/bwaindex/main.nf b/modules/nf-core/sentieon/bwaindex/main.nf index 0dde28d7..38e1d3a3 100644 --- a/modules/nf-core/sentieon/bwaindex/main.nf +++ b/modules/nf-core/sentieon/bwaindex/main.nf @@ -28,7 +28,7 @@ process SENTIEON_BWAINDEX { sentieon \\ bwa index \\ $args \\ - -p bwa/${fasta.baseName} \\ + -p $prefix \\ $fasta cat <<-END_VERSIONS > versions.yml From 6469c8aef6b8a5c5794c8aca3ba0cc3486a2f03e Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 8 Aug 2023 01:05:45 +0200 Subject: [PATCH 030/387] compressed outputs --- conf/modules/rank_variants.config | 16 ++++++++++++++++ conf/modules/raredisease.config | 8 ++++++++ modules/local/filter_vep.nf | 8 ++++---- subworkflows/local/rank_variants.nf | 4 ++++ workflows/raredisease.nf | 8 ++++++++ 5 files changed, 40 insertions(+), 4 deletions(-) diff --git a/conf/modules/rank_variants.config b/conf/modules/rank_variants.config index 1eee3385..352d618f 100644 --- a/conf/modules/rank_variants.config +++ b/conf/modules/rank_variants.config @@ -51,6 +51,14 @@ process { withName: '.*RANK_VARIANTS_SV:TABIX_BGZIP' { ext.when = false } + + withName: '.*RANK_VARIANTS_SV:TABIX_TABIX' { + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } // @@ -95,4 +103,12 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } + + withName: '.*RANK_VARIANTS_SNV:TABIX_TABIX' { + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } diff --git a/conf/modules/raredisease.config b/conf/modules/raredisease.config index db82ef14..69419687 100644 --- a/conf/modules/raredisease.config +++ b/conf/modules/raredisease.config @@ -105,6 +105,10 @@ process { process { withName: '.*FILTER_VEP_SNV' { ext.prefix = { "${meta.id}_clinical_snv" } + } + + withName: '.*BGZIPTABIX_SNV' { + ext.prefix = { "${meta.id}_clinical_snv" } publishDir = [ path: { "${params.outdir}/rank_and_filter" }, mode: params.publish_dir_mode, @@ -116,6 +120,10 @@ process { process { withName: '.*FILTER_VEP_SV' { ext.prefix = { "${meta.id}_clinical_sv" } + } + + withName: '.*BGZIPTABIX_SV' { + ext.prefix = { "${meta.id}_clinical_sv" } publishDir = [ path: { "${params.outdir}/rank_and_filter" }, mode: params.publish_dir_mode, diff --git a/modules/local/filter_vep.nf b/modules/local/filter_vep.nf index d2ffb904..3fbe8dad 100644 --- a/modules/local/filter_vep.nf +++ b/modules/local/filter_vep.nf @@ -13,8 +13,8 @@ process FILTER_VEP { path (select_feature_file) output: - tuple val(meta), path("*.ann_filter.vcf.gz"), emit: vcf - path "versions.yml" , emit: versions + tuple val(meta), path("*.ann_filter.vcf"), emit: vcf + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -26,7 +26,7 @@ process FILTER_VEP { filter_vep \\ --format vcf \\ --input_file $vcf \\ - --output_file ${prefix}.ann_filter.vcf.gz \\ + --output_file ${prefix}.ann_filter.vcf \\ --only_matched \\ --filter \"HGNC_ID in ${select_feature_file}\" @@ -39,7 +39,7 @@ process FILTER_VEP { stub: def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${prefix}.ann_filter.vcf.gz + touch ${prefix}.ann_filter.vcf cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/subworkflows/local/rank_variants.nf b/subworkflows/local/rank_variants.nf index 3049626f..cf8c6a8d 100644 --- a/subworkflows/local/rank_variants.nf +++ b/subworkflows/local/rank_variants.nf @@ -8,6 +8,7 @@ include { GENMOD_SCORE } from '../../modules/nf-core/genmod/score/main' include { GENMOD_COMPOUND } from '../../modules/nf-core/genmod/compound/main' include { BCFTOOLS_SORT } from '../../modules/nf-core/bcftools/sort/main' include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip/main' +include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix/main' workflow RANK_VARIANTS { @@ -34,12 +35,15 @@ workflow RANK_VARIANTS { ch_vcf = TABIX_BGZIP.out.output.mix(BCFTOOLS_SORT.out.vcf) + TABIX_TABIX (ch_vcf) + ch_versions = ch_versions.mix(GENMOD_ANNOTATE.out.versions) ch_versions = ch_versions.mix(GENMOD_MODELS.out.versions) ch_versions = ch_versions.mix(GENMOD_SCORE.out.versions) ch_versions = ch_versions.mix(GENMOD_COMPOUND.out.versions) ch_versions = ch_versions.mix(BCFTOOLS_SORT.out.versions) ch_versions = ch_versions.mix(TABIX_BGZIP.out.versions) + ch_versions = ch_versions.mix(TABIX_TABIX.out.versions) emit: vcf = ch_vcf // channel: [ val(meta), path(vcf) ] diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index dfdff09e..abd34e39 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -92,6 +92,8 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil include { FILTER_VEP as FILTER_VEP_SNV } from '../modules/local/filter_vep' include { FILTER_VEP as FILTER_VEP_SV } from '../modules/local/filter_vep' +include { TABIX_BGZIPTABIX as BGZIPTABIX_SNV } from '../modules/nf-core/tabix/bgziptabix' +include { TABIX_BGZIPTABIX as BGZIPTABIX_SV } from '../modules/nf-core/tabix/bgziptabix' // // MODULE: Installed directly from nf-core/modules @@ -436,6 +438,9 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(FILTER_VEP_SV.out.versions) + BGZIPTABIX_SV(FILTER_VEP_SV.out.vcf) + ch_versions = ch_versions.mix(BGZIPTABIX_SV.out.versions) + } if (!params.skip_mt_analysis) { @@ -536,6 +541,9 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(FILTER_VEP_SNV.out.versions) + BGZIPTABIX_SNV(FILTER_VEP_SNV.out.vcf) + ch_versions = ch_versions.mix(BGZIPTABIX_SNV.out.versions) + } // From 9f8ddf2b92addaebbf22b0d3a3465d22d0b95499 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 8 Aug 2023 11:45:17 +0200 Subject: [PATCH 031/387] review suggestion --- conf/modules/rank_variants.config | 1 - 1 file changed, 1 deletion(-) diff --git a/conf/modules/rank_variants.config b/conf/modules/rank_variants.config index 352d618f..64b7a53b 100644 --- a/conf/modules/rank_variants.config +++ b/conf/modules/rank_variants.config @@ -40,7 +40,6 @@ process { withName: '.*RANK_VARIANTS_SV:BCFTOOLS_SORT' { ext.args = "--output-type z" ext.prefix = { "${meta.id}_ranked_sv" } - ext.when = true publishDir = [ path: { "${params.outdir}/rank_and_filter" }, mode: params.publish_dir_mode, From afb19e7d862ed4b42e1d7021954fd4e3cf5e3f5e Mon Sep 17 00:00:00 2001 From: asp8200 Date: Tue, 8 Aug 2023 12:49:36 +0000 Subject: [PATCH 032/387] Adding sentieon/bwaindex to modules.json --- modules.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules.json b/modules.json index e2c0bb2f..30fc4d9d 100644 --- a/modules.json +++ b/modules.json @@ -337,7 +337,7 @@ }, "sentieon/bwaindex": { "branch": "master", - "git_sha": "b9172e8c26a3db5009f7872654c44587e254f094", + "git_sha": "49af8ed458e28729e483bc96e5a57c28163b9ea0", "installed_by": ["modules"] }, "smncopynumbercaller": { From c6b9ff67e99e9224ebf81b5098c469f96791dd49 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 9 Aug 2023 15:30:35 +0200 Subject: [PATCH 033/387] module update --- modules.json | 17 ++++-- modules/local/gatk4/collectreadcounts/main.nf | 56 ------------------ .../local/gatk4/collectreadcounts/meta.yml | 57 ------------------ .../local/gatk4/denoisereadcounts/meta.yml | 44 -------------- modules/nf-core/deepvariant/main.nf | 13 ++-- .../gatk4/denoisereadcounts/main.nf | 39 ++++++------ .../nf-core/gatk4/denoisereadcounts/meta.yml | 59 +++++++++++++++++++ .../determinegermlinecontigploidy/main.nf | 13 ++-- .../nf-core/gatk4/germlinecnvcaller/main.nf | 15 +++-- .../gatk4/postprocessgermlinecnvcalls/main.nf | 13 ++-- modules/nf-core/multiqc/main.nf | 6 +- modules/nf-core/untar/main.nf | 2 +- subworkflows/local/gens.nf | 4 +- 13 files changed, 131 insertions(+), 207 deletions(-) delete mode 100644 modules/local/gatk4/collectreadcounts/main.nf delete mode 100644 modules/local/gatk4/collectreadcounts/meta.yml delete mode 100644 modules/local/gatk4/denoisereadcounts/meta.yml rename modules/{local => nf-core}/gatk4/denoisereadcounts/main.nf (55%) create mode 100644 modules/nf-core/gatk4/denoisereadcounts/meta.yml diff --git a/modules.json b/modules.json index 38548e9b..5297fca0 100644 --- a/modules.json +++ b/modules.json @@ -82,7 +82,7 @@ }, "deepvariant": { "branch": "master", - "git_sha": "4b7d4863a5883b76e6bff13b6e52468fab090c5b", + "git_sha": "ed67f2fadd6d2a155b296f728e6b1f8c92ddc1a6", "installed_by": ["modules"] }, "eklipse": { @@ -115,9 +115,14 @@ "git_sha": "541811d779026c5d395925895fa5ed35e7216cc0", "installed_by": ["modules"] }, + "gatk4/denoisereadcounts": { + "branch": "master", + "git_sha": "38c559b2a8b9a8cda62fa215521f581eb979c773", + "installed_by": ["modules"] + }, "gatk4/determinegermlinecontigploidy": { "branch": "master", - "git_sha": "c6257c167fb88d18f3c2797e1af331d28f7b8ae7", + "git_sha": "8c4542e5d421c4690cf1fa6ec729e9304763fdaf", "installed_by": ["modules"] }, "gatk4/filtermutectcalls": { @@ -127,7 +132,7 @@ }, "gatk4/germlinecnvcaller": { "branch": "master", - "git_sha": "f6b848c6e1af9a9ecf4975aa8c8edad05e75e784", + "git_sha": "8c4542e5d421c4690cf1fa6ec729e9304763fdaf", "installed_by": ["modules"] }, "gatk4/intervallisttools": { @@ -152,7 +157,7 @@ }, "gatk4/postprocessgermlinecnvcalls": { "branch": "master", - "git_sha": "c6257c167fb88d18f3c2797e1af331d28f7b8ae7", + "git_sha": "8c4542e5d421c4690cf1fa6ec729e9304763fdaf", "installed_by": ["modules"] }, "gatk4/preprocessintervals": { @@ -247,7 +252,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "a6e11ac655e744f7ebc724be669dd568ffdc0e80", "installed_by": ["modules"] }, "peddy": { @@ -382,7 +387,7 @@ }, "untar": { "branch": "master", - "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", + "git_sha": "d0b4fc03af52a1cc8c6fb4493b921b57352b1dd8", "installed_by": ["modules"] }, "upd": { diff --git a/modules/local/gatk4/collectreadcounts/main.nf b/modules/local/gatk4/collectreadcounts/main.nf deleted file mode 100644 index f424d20e..00000000 --- a/modules/local/gatk4/collectreadcounts/main.nf +++ /dev/null @@ -1,56 +0,0 @@ -process GATK4_COLLECTREADCOUNTS { - tag "$meta.id" - label 'process_medium' - - conda "bioconda::gatk4=4.4.0.0" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'quay.io/biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" - - input: - tuple val(meta), path(bam), path(bai) - path fasta - path fai - path sequence_dict - path interval_list - - output: - tuple val(meta), path('*.hdf5'), emit: read_counts - path "versions.yml" , emit: versions - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def avail_mem = 12288 - if (!task.memory) { - log.info '[GATK CollectReadCounts] Available memory not known - defaulting to 12GB. Specify process memory requirements to change this.' - } else { - avail_mem = (task.memory.mega*0.8).intValue() - } - """ - gatk --java-options "-Xmx${avail_mem}M" CollectReadCounts \\ - -I $bam \\ - --read-index $bai \\ - -R $fasta \\ - -L $interval_list \\ - -O ${prefix}.hdf5 \\ - $args \\ - --tmp-dir . - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.hdf5 - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') - END_VERSIONS - """ -} diff --git a/modules/local/gatk4/collectreadcounts/meta.yml b/modules/local/gatk4/collectreadcounts/meta.yml deleted file mode 100644 index 6da91b16..00000000 --- a/modules/local/gatk4/collectreadcounts/meta.yml +++ /dev/null @@ -1,57 +0,0 @@ -name: gatk4_collectreadcounts -description: -keywords: - - bam - - interval list - - hdf5 -tools: - - gatk4: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 - licence: ["Apache-2.0"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test'] - - bam: - type: file - description: BAM/CRAM file from alignment - pattern: "*.{bam,cram}" - - bai: - type: file - description: BAI/CRAI file from alignment - pattern: "*.{bai,crai}" - - fasta: - type: file - description: The reference fasta file - pattern: "*.fasta" - - fai: - type: file - description: Index of reference fasta file - pattern: "fasta.fai" - - sequence_dict: - type: file - description: GATK sequence dictionary - pattern: "*.dict" - - interval_list: - type: file - description: Binning intervals file - pattern: "*.interval_list" -output: - - read_counts: - type: file - description: gatk read count file - pattern: "*.hdf5" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@raysloks" diff --git a/modules/local/gatk4/denoisereadcounts/meta.yml b/modules/local/gatk4/denoisereadcounts/meta.yml deleted file mode 100644 index 6e343e8e..00000000 --- a/modules/local/gatk4/denoisereadcounts/meta.yml +++ /dev/null @@ -1,44 +0,0 @@ -name: gatk4_denoisereadcounts -description: -keywords: - - hdf5 - - interval list -tools: - - gatk4: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 - licence: ["Apache-2.0"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test'] - - read_counts: - type: file - description: Input read counts file - pattern: "*.hdf5" - - read_counts_panel: - type: file - description: Panel of normals - pattern: "*.hdf5" - - interval_list: - type: file - description: Binning intervals file - pattern: "*.interval_list" -output: - - read_counts: - type: file - description: gatk read count file - pattern: "*.hdf5" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@raysloks" diff --git a/modules/nf-core/deepvariant/main.nf b/modules/nf-core/deepvariant/main.nf index 1a24ba34..2d5c480c 100644 --- a/modules/nf-core/deepvariant/main.nf +++ b/modules/nf-core/deepvariant/main.nf @@ -4,11 +4,6 @@ process DEEPVARIANT { container "nf-core/deepvariant:1.5.0" - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - exit 1, "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead." - } - input: tuple val(meta), path(input), path(index), path(intervals) tuple val(meta2), path(fasta) @@ -26,6 +21,10 @@ process DEEPVARIANT { task.ext.when == null || task.ext.when script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead." + } def args = task.ext.args ?: '' prefix = task.ext.prefix ?: "${meta.id}" def regions = intervals ? "--regions=${intervals}" : "" @@ -48,6 +47,10 @@ process DEEPVARIANT { """ stub: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead." + } prefix = task.ext.prefix ?: "${meta.id}" """ touch ${prefix}.vcf.gz diff --git a/modules/local/gatk4/denoisereadcounts/main.nf b/modules/nf-core/gatk4/denoisereadcounts/main.nf similarity index 55% rename from modules/local/gatk4/denoisereadcounts/main.nf rename to modules/nf-core/gatk4/denoisereadcounts/main.nf index c18a7763..e60aeda1 100644 --- a/modules/local/gatk4/denoisereadcounts/main.nf +++ b/modules/nf-core/gatk4/denoisereadcounts/main.nf @@ -1,38 +1,42 @@ process GATK4_DENOISEREADCOUNTS { tag "$meta.id" - label 'process_high' + label 'process_single' conda "bioconda::gatk4=4.4.0.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'quay.io/biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" input: - tuple val(meta), path(read_counts) - path panel_of_normals + tuple val(meta), path(counts) + tuple val(meta2), path(pon) output: - tuple val(meta), path('*.standardizedCR.tsv'), emit: standardized_read_counts - tuple val(meta), path('*.denoisedCR.tsv') , emit: denoised_read_counts - path "versions.yml" , emit: versions + tuple val(meta), path("*_standardizedCR.tsv"), emit: standardized + tuple val(meta), path("*_denoisedCR.tsv") , emit: denoised + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def avail_mem = 12288 + + def avail_mem = 3072 if (!task.memory) { - log.info '[GATK DenoiseReadCounts] Available memory not known - defaulting to 12GB. Specify process memory requirements to change this.' + log.info '[GATK DenoiseReadCounts] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { avail_mem = (task.memory.mega*0.8).intValue() } """ gatk --java-options "-Xmx${avail_mem}M" DenoiseReadCounts \\ - -I $read_counts \\ - --count-panel-of-normals $panel_of_normals \\ - --standardized-copy-ratios ${prefix}.standardizedCR.tsv \\ - --denoised-copy-ratios ${prefix}.denoisedCR.tsv \\ - $args \\ - --tmp-dir . + ${args} \\ + --tmp-dir . \\ + --input ${counts} \\ + --count-panel-of-normals ${pon} \\ + --standardized-copy-ratios ${prefix}_standardizedCR.tsv \\ + --denoised-copy-ratios ${prefix}_denoisedCR.tsv cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -41,10 +45,11 @@ process GATK4_DENOISEREADCOUNTS { """ stub: + def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${prefix}.standardizedCR.tsv - touch ${prefix}.denoisedCR.tsv + touch ${prefix}_standardizedCR.tsv + touch ${prefix}_denoisedCR.tsv cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/gatk4/denoisereadcounts/meta.yml b/modules/nf-core/gatk4/denoisereadcounts/meta.yml new file mode 100644 index 00000000..0bb2f389 --- /dev/null +++ b/modules/nf-core/gatk4/denoisereadcounts/meta.yml @@ -0,0 +1,59 @@ +name: "gatk4_denoisereadcounts" +description: Denoises read counts to produce denoised copy ratios +keywords: + - gatk4 + - denoisereadcounts + - copyratios +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + tool_dev_url: "https://github.com/broadinstitute/gatk" + licence: ["Apache-2.0"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - meta2: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - counts: + type: file + description: Read counts in hdf5 or tsv format. + pattern: "*.{hdf5,tsv}" + - pon: + type: file + description: Panel of normals file hdf5 or tsv format. + pattern: "*.{hdf5}" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - standardized: + type: file + description: Standardized copy ratios file. + pattern: "*.{tsv}" + - denoised: + type: file + description: Denoised copy ratios file + pattern: "*.{tsv}" + +authors: + - "@ramprasadn" diff --git a/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf b/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf index 407cc34c..3c21e743 100644 --- a/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf +++ b/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf @@ -6,11 +6,6 @@ process GATK4_DETERMINEGERMLINECONTIGPLOIDY { //Conda is not supported at the moment: https://github.com/broadinstitute/gatk/issues/7811 container "nf-core/gatk:4.4.0.0" //Biocontainers is missing a package - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - exit 1, "GATK4_DETERMINEGERMLINECONTIGPLOIDY module does not support Conda. Please use Docker / Singularity / Podman instead." - } - input: tuple val(meta), path(counts), path(bed), path(exclude_beds) tuple val(meta2), path(ploidy_model) @@ -25,6 +20,10 @@ process GATK4_DETERMINEGERMLINECONTIGPLOIDY { task.ext.when == null || task.ext.when script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "GATK4_DETERMINEGERMLINECONTIGPLOIDY module does not support Conda. Please use Docker / Singularity / Podman instead." + } def args = task.ext.args ?: '' prefix = task.ext.prefix ?: "${meta.id}" def intervals = bed ? "--intervals ${bed}" : "" @@ -58,6 +57,10 @@ process GATK4_DETERMINEGERMLINECONTIGPLOIDY { """ stub: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "GATK4_DETERMINEGERMLINECONTIGPLOIDY module does not support Conda. Please use Docker / Singularity / Podman instead." + } prefix = task.ext.prefix ?: "${meta.id}" """ touch ${prefix}-calls diff --git a/modules/nf-core/gatk4/germlinecnvcaller/main.nf b/modules/nf-core/gatk4/germlinecnvcaller/main.nf index 9b31c56d..973a0d3b 100644 --- a/modules/nf-core/gatk4/germlinecnvcaller/main.nf +++ b/modules/nf-core/gatk4/germlinecnvcaller/main.nf @@ -3,12 +3,7 @@ process GATK4_GERMLINECNVCALLER { label 'process_single' //Conda is not supported at the moment: https://github.com/broadinstitute/gatk/issues/7811 - container "quay.io/nf-core/gatk:4.4.0.0" //Biocontainers is missing a package - - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - exit 1, "GATK4_GERMLINECNVCALLER module does not support Conda. Please use Docker / Singularity / Podman instead." - } + container "nf-core/gatk:4.4.0.0" //Biocontainers is missing a package input: tuple val(meta), path(tsv), path(intervals), path(ploidy), path(model) @@ -22,6 +17,10 @@ process GATK4_GERMLINECNVCALLER { task.ext.when == null || task.ext.when script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "GATK4_GERMLINECNVCALLER module does not support Conda. Please use Docker / Singularity / Podman instead." + } def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def intervals_command = intervals ? "--intervals ${intervals}" : "" @@ -53,6 +52,10 @@ process GATK4_GERMLINECNVCALLER { """ stub: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "GATK4_GERMLINECNVCALLER module does not support Conda. Please use Docker / Singularity / Podman instead." + } def prefix = task.ext.prefix ?: "${meta.id}" """ mkdir -p ${prefix}-cnv-calls/${prefix}-calls diff --git a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf index d1a03fc1..d6223043 100644 --- a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf +++ b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf @@ -5,11 +5,6 @@ process GATK4_POSTPROCESSGERMLINECNVCALLS { //Conda is not supported at the moment: https://github.com/broadinstitute/gatk/issues/7811 container "nf-core/gatk:4.4.0.0" //Biocontainers is missing a package - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - exit 1, "GATK4_POSTPROCESSGERMLINECNVCALLS module does not support Conda. Please use Docker / Singularity / Podman instead." - } - input: tuple val(meta), path(calls), path(model), path(ploidy) @@ -23,6 +18,10 @@ process GATK4_POSTPROCESSGERMLINECNVCALLS { task.ext.when == null || task.ext.when script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "GATK4_POSTPROCESSGERMLINECNVCALLS module does not support Conda. Please use Docker / Singularity / Podman instead." + } def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def calls_command = calls ? calls.collect{"--calls-shard-path $it"}.join(' ') : "" @@ -51,6 +50,10 @@ process GATK4_POSTPROCESSGERMLINECNVCALLS { """ stub: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "GATK4_POSTPROCESSGERMLINECNVCALLS module does not support Conda. Please use Docker / Singularity / Podman instead." + } def prefix = task.ext.prefix ?: "${meta.id}" """ touch ${prefix}_genotyped_intervals.vcf.gz diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1fc387be..65d7dd0d 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -1,10 +1,10 @@ process MULTIQC { label 'process_single' - conda "bioconda::multiqc=1.14" + conda "bioconda::multiqc=1.15" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.15--pyhdfd78af_0' : + 'biocontainers/multiqc:1.15--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf index 8cd1856c..61461c39 100644 --- a/modules/nf-core/untar/main.nf +++ b/modules/nf-core/untar/main.nf @@ -2,7 +2,7 @@ process UNTAR { tag "$archive" label 'process_single' - conda "conda-forge::sed=4.7 bioconda::grep=3.4 conda-forge::tar=1.34" + conda "conda-forge::sed=4.7 conda-forge::grep=3.11 conda-forge::tar=1.34" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : 'nf-core/ubuntu:20.04' }" diff --git a/subworkflows/local/gens.nf b/subworkflows/local/gens.nf index 8e47cac7..fdee366d 100644 --- a/subworkflows/local/gens.nf +++ b/subworkflows/local/gens.nf @@ -2,8 +2,8 @@ // A preprocessing workflow for Gens // -include { GATK4_COLLECTREADCOUNTS as COLLECTREADCOUNTS } from '../../modules/local/gatk4/collectreadcounts/main' -include { GATK4_DENOISEREADCOUNTS as DENOISEREADCOUNTS } from '../../modules/local/gatk4/denoisereadcounts/main' +include { GATK4_COLLECTREADCOUNTS as COLLECTREADCOUNTS } from '../../modules/nf-core/gatk4/collectreadcounts/main' +include { GATK4_DENOISEREADCOUNTS as DENOISEREADCOUNTS } from '../../modules/nf-core/gatk4/denoisereadcounts/main' include { GENS as GENS_GENERATE } from '../../modules/local/gens/main' workflow GENS { From 90e1861d08ec947a088cab879832f945c9b60885 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Wed, 9 Aug 2023 14:39:18 +0000 Subject: [PATCH 034/387] Installing and calling nf-core/modules version of sentieon/bwamem --- conf/modules/align_sentieon.config | 5 ++ modules.json | 5 ++ modules/local/sentieon/bwamem.nf | 68 ---------------- modules/nf-core/sentieon/bwamem/main.nf | 78 +++++++++++++++++++ modules/nf-core/sentieon/bwamem/meta.yml | 63 +++++++++++++++ .../local/alignment/align_sentieon.nf | 7 +- .../local/mitochondria/align_and_call_MT.nf | 6 +- 7 files changed, 157 insertions(+), 75 deletions(-) delete mode 100644 modules/local/sentieon/bwamem.nf create mode 100644 modules/nf-core/sentieon/bwamem/main.nf create mode 100644 modules/nf-core/sentieon/bwamem/meta.yml diff --git a/conf/modules/align_sentieon.config b/conf/modules/align_sentieon.config index a882a017..b2af6b6e 100644 --- a/conf/modules/align_sentieon.config +++ b/conf/modules/align_sentieon.config @@ -16,6 +16,11 @@ // process { + withLabel: 'sentieon' { + ext.sentieon_auth_mech_base64 = secrets.SENTIEON_AUTH_MECH_BASE64 + ext.sentieon_auth_data_base64 = secrets.SENTIEON_AUTH_DATA_BASE64 + } + withName: '.*ALIGN_SENTIEON:.*' { ext.when = params.aligner.equals("sentieon") } diff --git a/modules.json b/modules.json index 0b3f5084..106948ad 100644 --- a/modules.json +++ b/modules.json @@ -345,6 +345,11 @@ "git_sha": "49af8ed458e28729e483bc96e5a57c28163b9ea0", "installed_by": ["modules"] }, + "sentieon/bwamem": { + "branch": "master", + "git_sha": "b9172e8c26a3db5009f7872654c44587e254f094", + "installed_by": ["modules"] + }, "smncopynumbercaller": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", diff --git a/modules/local/sentieon/bwamem.nf b/modules/local/sentieon/bwamem.nf deleted file mode 100644 index 60ca36d6..00000000 --- a/modules/local/sentieon/bwamem.nf +++ /dev/null @@ -1,68 +0,0 @@ -process SENTIEON_BWAMEM { - tag "$meta.id" - label 'process_high' - label 'sentieon' - - secret 'SENTIEON_LICENSE_BASE64' - - input: - tuple val(meta), path(reads) - tuple val(meta2), path(fasta) - tuple val(meta3), path(fai) - tuple val(meta4), path(index) - - output: - tuple val(meta), path('*.bam'), emit: bam - tuple val(meta), path('*.bai'), emit: bai - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - INDEX=`find -L ./ -name "*.amb" | sed 's/.amb//'` - - if [ \${SENTIEON_LICENSE_BASE64:-"unset"} != "unset" ]; then - echo "Initializing SENTIEON_LICENSE env variable" - source sentieon_init.sh SENTIEON_LICENSE_BASE64 - fi - - sentieon bwa mem \\ - -t $task.cpus \\ - \$INDEX \\ - $reads \\ - $args \\ - | sentieon \\ - util \\ - sort \\ - -r $fasta \\ - -o ${prefix}.bam \\ - -t $task.cpus \\ - $args2 \\ - --sam2bam \\ - -i - - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - bwa: \$(echo \$(sentieon bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.bam - touch ${prefix}.bai - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - bwa: \$(echo \$(sentieon bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') - END_VERSIONS - """ -} diff --git a/modules/nf-core/sentieon/bwamem/main.nf b/modules/nf-core/sentieon/bwamem/main.nf new file mode 100644 index 00000000..aeca2825 --- /dev/null +++ b/modules/nf-core/sentieon/bwamem/main.nf @@ -0,0 +1,78 @@ +process SENTIEON_BWAMEM { + tag "$meta.id" + label 'process_high' + label 'sentieon' + + secret 'SENTIEON_LICENSE_BASE64' + + container 'nf-core/sentieon:202112.06' + + input: + tuple val(meta), path(reads) + tuple val(meta2), path(index) + path(fasta) + path(fasta_fai) + + output: + tuple val(meta), path("*.bam"), path("*.bai"), emit: bam_and_bai + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + } + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: '' + def sentieon_auth_data_base64 = task.ext.sentieon_auth_data_base64 ?: '' + + """ + if [ "\${#SENTIEON_LICENSE_BASE64}" -lt "1500" ]; then # If the string SENTIEON_LICENSE_BASE64 is short, then it is an encrypted url. + export SENTIEON_LICENSE=\$(echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d) + else # Localhost license file + # The license file is stored as a nextflow variable like, for instance, this: + # nextflow secrets set SENTIEON_LICENSE_BASE64 \$(cat | base64 -w 0) + export SENTIEON_LICENSE=\$(mktemp) + echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d > \$SENTIEON_LICENSE + fi + + if [ ${sentieon_auth_mech_base64} ] && [ ${sentieon_auth_data_base64} ]; then + # If sentieon_auth_mech_base64 and sentieon_auth_data_base64 are non-empty strings, then Sentieon is mostly likely being run with some test-license. + export SENTIEON_AUTH_MECH=\$(echo -n "${sentieon_auth_mech_base64}" | base64 -d) + export SENTIEON_AUTH_DATA=\$(echo -n "${sentieon_auth_data_base64}" | base64 -d) + echo "Decoded and exported Sentieon test-license system environment variables" + fi + + INDEX=`find -L ./ -name "*.amb" | sed 's/.amb//'` + + sentieon bwa mem \\ + $args \\ + -t $task.cpus \\ + \$INDEX \\ + $reads \\ + | sentieon util sort -r $fasta -t $task.cpus -o ${prefix}.bam --sam2bam - + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") + bwa: \$(echo \$(sentieon bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bam + touch ${prefix}.bam.bai + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") + bwa: \$(echo \$(sentieon bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/sentieon/bwamem/meta.yml b/modules/nf-core/sentieon/bwamem/meta.yml new file mode 100644 index 00000000..3d1546c5 --- /dev/null +++ b/modules/nf-core/sentieon/bwamem/meta.yml @@ -0,0 +1,63 @@ +name: sentieon_bwamem +description: Performs fastq alignment to a fasta reference using Sentieon's BWA MEM +keywords: + - mem + - bwa + - alignment + - map + - fastq + - bam + - sentieon +tools: + - sentieon: + description: | + Sentieon® provides complete solutions for secondary DNA/RNA analysis for a variety of sequencing platforms, including short and long reads. + Our software improves upon BWA, STAR, Minimap2, GATK, HaplotypeCaller, Mutect, and Mutect2 based pipelines and is deployable on any generic-CPU-based computing system. + homepage: https://www.sentieon.com/ + documentation: https://www.sentieon.com/ +input: + - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: Genome fastq files (single-end or paired-end) + - meta2: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - index: + type: file + description: BWA genome index files + pattern: "*.{amb,ann,bwt,pac,sa}" + - fasta: + type: file + description: Genome fasta file + pattern: "*.{fa,fasta}" + - fasta_fai: + type: file + description: The index of the FASTA reference. + pattern: "*.fai" +output: + - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file. + pattern: "*.bam" + - bai: + type: file + description: BAI file + pattern: "*.bai" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@asp8200" diff --git a/subworkflows/local/alignment/align_sentieon.nf b/subworkflows/local/alignment/align_sentieon.nf index e4094edf..796aeaa4 100644 --- a/subworkflows/local/alignment/align_sentieon.nf +++ b/subworkflows/local/alignment/align_sentieon.nf @@ -2,7 +2,7 @@ // A subworkflow to annotate structural variants. // -include { SENTIEON_BWAMEM } from '../../../modules/local/sentieon/bwamem' +include { SENTIEON_BWAMEM } from '../../../modules/nf-core/sentieon/bwamem/main' include { SENTIEON_DATAMETRICS } from '../../../modules/local/sentieon/datametrics' include { SENTIEON_LOCUSCOLLECTOR } from '../../../modules/local/sentieon/locuscollector' include { SENTIEON_DEDUP } from '../../../modules/local/sentieon/dedup' @@ -25,11 +25,10 @@ workflow ALIGN_SENTIEON { ch_bqsr_bai = Channel.empty() ch_bqsr_csv = Channel.empty() - SENTIEON_BWAMEM ( ch_reads_input, ch_genome_fasta, ch_genome_fai, ch_bwa_index ) + SENTIEON_BWAMEM ( ch_reads_input, ch_bwa_index, ch_genome_fasta.map{ meta, fasta -> fasta }, ch_genome_fai.map{ meta, fai -> fai }) SENTIEON_BWAMEM.out - .bam - .join(SENTIEON_BWAMEM.out.bai, failOnMismatch:true, failOnDuplicate:true) + .bam_and_bai .map{ meta, bam, bai -> new_id = meta.id.split('_')[0] new_meta = meta + [id:new_id, read_group:"\'@RG\\tID:" + new_id + "\\tPL:" + val_platform + "\\tSM:" + new_id + "\'"] diff --git a/subworkflows/local/mitochondria/align_and_call_MT.nf b/subworkflows/local/mitochondria/align_and_call_MT.nf index be060a7d..fb00739a 100644 --- a/subworkflows/local/mitochondria/align_and_call_MT.nf +++ b/subworkflows/local/mitochondria/align_and_call_MT.nf @@ -2,7 +2,7 @@ // Align and call MT // -include { SENTIEON_BWAMEM as SENTIEON_BWAMEM_MT } from '../../../modules/local/sentieon/bwamem' +include { SENTIEON_BWAMEM as SENTIEON_BWAMEM_MT } from '../../../modules/nf-core/sentieon/bwamem/main' include { BWAMEM2_MEM as BWAMEM2_MEM_MT } from '../../../modules/nf-core/bwamem2/mem/main' include { GATK4_MERGEBAMALIGNMENT as GATK4_MERGEBAMALIGNMENT_MT } from '../../../modules/nf-core/gatk4/mergebamalignment/main' include { PICARD_ADDORREPLACEREADGROUPS as PICARD_ADDORREPLACEREADGROUPS_MT } from '../../../modules/nf-core/picard/addorreplacereadgroups/main' @@ -32,10 +32,10 @@ workflow ALIGN_AND_CALL_MT { BWAMEM2_MEM_MT (ch_fastq, ch_bwamem2index, true) - SENTIEON_BWAMEM_MT ( ch_fastq, ch_fasta, ch_fai, ch_bwaindex ) + SENTIEON_BWAMEM_MT ( ch_fastq, ch_bwaindex, ch_fasta.map{ meta, fasta -> fasta }, ch_fai.map{ meta, fai -> fai } ) Channel.empty() - .mix(BWAMEM2_MEM_MT.out.bam, SENTIEON_BWAMEM_MT.out.bam) + .mix(BWAMEM2_MEM_MT.out.bam, SENTIEON_BWAMEM_MT.out.bam_and_bai.map{ meta, bam, bai -> [meta, bam] }) .join(ch_ubam, failOnMismatch:true, failOnDuplicate:true) .set {ch_bam_ubam} From 78eaee681429eb3cada860854d4e61d7b672a80e Mon Sep 17 00:00:00 2001 From: asp8200 Date: Wed, 9 Aug 2023 15:30:13 +0000 Subject: [PATCH 035/387] Moving setup of sentieon-secrets to test.config --- conf/modules/align_sentieon.config | 4 ---- conf/test.config | 7 +++++++ 2 files changed, 7 insertions(+), 4 deletions(-) diff --git a/conf/modules/align_sentieon.config b/conf/modules/align_sentieon.config index b2af6b6e..5d89f69c 100644 --- a/conf/modules/align_sentieon.config +++ b/conf/modules/align_sentieon.config @@ -16,10 +16,6 @@ // process { - withLabel: 'sentieon' { - ext.sentieon_auth_mech_base64 = secrets.SENTIEON_AUTH_MECH_BASE64 - ext.sentieon_auth_data_base64 = secrets.SENTIEON_AUTH_DATA_BASE64 - } withName: '.*ALIGN_SENTIEON:.*' { ext.when = params.aligner.equals("sentieon") diff --git a/conf/test.config b/conf/test.config index 8065aa8a..9093bfb6 100644 --- a/conf/test.config +++ b/conf/test.config @@ -50,3 +50,10 @@ params { vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" vep_cache_version = 107 } + +process { + withLabel: 'sentieon' { + ext.sentieon_auth_mech_base64 = secrets.SENTIEON_AUTH_MECH_BASE64 + ext.sentieon_auth_data_base64 = secrets.SENTIEON_AUTH_DATA_BASE64 + } +} From 219fc4221aaac26d1664b3e85ec19fc1ed22b1de Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 14 Aug 2023 09:13:35 +0000 Subject: [PATCH 036/387] Replace local version of SENTIEON_READWRITER with nf-core version --- modules.json | 5 ++ modules/local/sentieon/readwriter.nf | 54 ------------ modules/nf-core/sentieon/readwriter/main.nf | 84 +++++++++++++++++++ modules/nf-core/sentieon/readwriter/meta.yml | 71 ++++++++++++++++ .../local/alignment/align_sentieon.nf | 5 +- 5 files changed, 162 insertions(+), 57 deletions(-) delete mode 100644 modules/local/sentieon/readwriter.nf create mode 100644 modules/nf-core/sentieon/readwriter/main.nf create mode 100644 modules/nf-core/sentieon/readwriter/meta.yml diff --git a/modules.json b/modules.json index 106948ad..5f9df891 100644 --- a/modules.json +++ b/modules.json @@ -350,6 +350,11 @@ "git_sha": "b9172e8c26a3db5009f7872654c44587e254f094", "installed_by": ["modules"] }, + "sentieon/readwriter": { + "branch": "master", + "git_sha": "b28e4dde755117e8dab5d6e85e292f145b8b53c3", + "installed_by": ["modules"] + }, "smncopynumbercaller": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", diff --git a/modules/local/sentieon/readwriter.nf b/modules/local/sentieon/readwriter.nf deleted file mode 100644 index cc90fd25..00000000 --- a/modules/local/sentieon/readwriter.nf +++ /dev/null @@ -1,54 +0,0 @@ -process SENTIEON_READWRITER { - tag "$meta.id" - label 'process_medium' - label 'sentieon' - - secret 'SENTIEON_LICENSE_BASE64' - - input: - tuple val(meta), path(bam), path(bai) - - output: - tuple val(meta), path('*.bam') , emit: bam - tuple val(meta), path('*.bam.bai') , emit: bai - tuple val(meta), path('*.bam'), path('*.bam.bai'), emit: bam_bai - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def input = bam.sort().collect{"-i $it"}.join(' ') - def prefix = task.ext.prefix ?: "${meta.id}" - """ - if [ \${SENTIEON_LICENSE_BASE64:-"unset"} != "unset" ]; then - echo "Initializing SENTIEON_LICENSE env variable" - source sentieon_init.sh SENTIEON_LICENSE_BASE64 - fi - - sentieon \\ - driver \\ - -t $task.cpus \\ - $input \\ - --algo ReadWriter \\ - ${prefix}.bam - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.bam - touch ${prefix}.bam.bai - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - END_VERSIONS - """ -} diff --git a/modules/nf-core/sentieon/readwriter/main.nf b/modules/nf-core/sentieon/readwriter/main.nf new file mode 100644 index 00000000..080b3ad7 --- /dev/null +++ b/modules/nf-core/sentieon/readwriter/main.nf @@ -0,0 +1,84 @@ +process SENTIEON_READWRITER { + tag "$meta.id" + label 'process_medium' + label 'sentieon' + + secret 'SENTIEON_LICENSE_BASE64' + + container 'nf-core/sentieon:202112.06' + + input: + tuple val(meta), path(input), path(index) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fai) + + output: + tuple val(meta), path("*.${format}"), emit: output + tuple val(meta), path("*.${index}") , emit: index + tuple val(meta), path("*.${format}"), path("*.${index}"), emit: output_index + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + } + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def input_str = input.sort().collect{"-i $it"}.join(' ') + def reference = fasta ? "-r $fasta" : '' + def prefix = task.ext.prefix ?: "${meta.id}" + format = input.extension == "bam" ? "bam" : "cram" + index = format == "bam" ? "bam.bai" : "cram.crai" + def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: '' + def sentieon_auth_data_base64 = task.ext.sentieon_auth_data_base64 ?: '' + """ + if [ "\${#SENTIEON_LICENSE_BASE64}" -lt "1500" ]; then # If the string SENTIEON_LICENSE_BASE64 is short, then it is an encrypted url. + export SENTIEON_LICENSE=\$(echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d) + else # Localhost license file + # The license file is stored as a nextflow variable like, for instance, this: + # nextflow secrets set SENTIEON_LICENSE_BASE64 \$(cat | base64 -w 0) + export SENTIEON_LICENSE=\$(mktemp) + echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d > \$SENTIEON_LICENSE + fi + + if [ ${sentieon_auth_mech_base64} ] && [ ${sentieon_auth_data_base64} ]; then + # If sentieon_auth_mech_base64 and sentieon_auth_data_base64 are non-empty strings, then Sentieon is mostly likely being run with some test-license. + export SENTIEON_AUTH_MECH=\$(echo -n "${sentieon_auth_mech_base64}" | base64 -d) + export SENTIEON_AUTH_DATA=\$(echo -n "${sentieon_auth_data_base64}" | base64 -d) + echo "Decoded and exported Sentieon test-license system environment variables" + fi + + sentieon \\ + driver \\ + -t $task.cpus \\ + $reference \\ + $args \\ + $input_str \\ + --algo ReadWriter \\ + $args2 \\ + ${prefix}.${format} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + format = input.extension == "bam" ? "bam" : "cram" + index = format == "bam" ? "bam.bai" : "cram.crai" + """ + touch ${prefix}.${format} + touch ${prefix}.${index} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/sentieon/readwriter/meta.yml b/modules/nf-core/sentieon/readwriter/meta.yml new file mode 100644 index 00000000..23dcfcab --- /dev/null +++ b/modules/nf-core/sentieon/readwriter/meta.yml @@ -0,0 +1,71 @@ +name: sentieon_readwriter +description: Merges BAM files, and/or convert them into cram files. Also, outputs the result of applying the Base Quality Score Recalibration to a file. +keywords: + - merge + - convert + - readwriter + - sentieon +tools: + - sentieon: + description: | + Sentieon® provides complete solutions for secondary DNA/RNA analysis for a variety of sequencing platforms, including short and long reads. + Our software improves upon BWA, STAR, Minimap2, GATK, HaplotypeCaller, Mutect, and Mutect2 based pipelines and is deployable on any generic-CPU-based computing system. + homepage: https://www.sentieon.com/ + documentation: https://www.sentieon.com/ +input: + - meta: + type: map + description: | + Groovy Map containing sample information. + e.g. [ id:'test', single_end:false ] + - meta2: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test' ] + - meta3: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test' ] + - input: + type: file + description: BAM/CRAM file. + pattern: "*.{bam,cram}" + - index: + type: file + description: BAI/CRAI file. + pattern: "*.{bai,crai}" + - fasta: + type: file + description: Genome fasta file + pattern: "*.{fa,fasta}" + - fai: + type: file + description: The index of the FASTA reference. + pattern: "*.fai" + +output: + - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - output: + type: file + description: BAM/CRAM file + pattern: "*.{bam,cram}" + - index: + type: file + description: BAM/CRAM index file + pattern: "*.{bai,crai}" + - output_index: + type: file + description: BAM/CRAM alignment and the corresponding index file + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + +authors: + - "@ramprasadn" diff --git a/subworkflows/local/alignment/align_sentieon.nf b/subworkflows/local/alignment/align_sentieon.nf index 796aeaa4..2f172714 100644 --- a/subworkflows/local/alignment/align_sentieon.nf +++ b/subworkflows/local/alignment/align_sentieon.nf @@ -7,8 +7,7 @@ include { SENTIEON_DATAMETRICS } from '../../../modules/local/sentieon/datame include { SENTIEON_LOCUSCOLLECTOR } from '../../../modules/local/sentieon/locuscollector' include { SENTIEON_DEDUP } from '../../../modules/local/sentieon/dedup' include { SENTIEON_BQSR } from '../../../modules/local/sentieon/bqsr' -include { SENTIEON_READWRITER } from '../../../modules/local/sentieon/readwriter' - +include { SENTIEON_READWRITER } from '../../../modules/nf-core/sentieon/readwriter/main' workflow ALIGN_SENTIEON { take: ch_reads_input // channel: [mandatory] [ val(meta), path(reads_input) ] @@ -41,7 +40,7 @@ workflow ALIGN_SENTIEON { } .set{ merge_bams_in } - SENTIEON_READWRITER (merge_bams_in.multiple) + SENTIEON_READWRITER ( merge_bams_in.multiple, ch_genome_fasta, ch_genome_fai ) ch_bam_bai = merge_bams_in.single.mix(SENTIEON_READWRITER.out.bam_bai) SENTIEON_DATAMETRICS (ch_bam_bai, ch_genome_fasta, ch_genome_fai ) From 82ec0b23eb9fd7b18bb6cd3ae37e116976055b6c Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 14 Aug 2023 09:23:05 +0000 Subject: [PATCH 037/387] Fixing name of output-channel from SENTIEON_READWRITER --- subworkflows/local/alignment/align_sentieon.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/alignment/align_sentieon.nf b/subworkflows/local/alignment/align_sentieon.nf index 2f172714..25673f2e 100644 --- a/subworkflows/local/alignment/align_sentieon.nf +++ b/subworkflows/local/alignment/align_sentieon.nf @@ -41,7 +41,7 @@ workflow ALIGN_SENTIEON { .set{ merge_bams_in } SENTIEON_READWRITER ( merge_bams_in.multiple, ch_genome_fasta, ch_genome_fai ) - ch_bam_bai = merge_bams_in.single.mix(SENTIEON_READWRITER.out.bam_bai) + ch_bam_bai = merge_bams_in.single.mix(SENTIEON_READWRITER.out.output_index) SENTIEON_DATAMETRICS (ch_bam_bai, ch_genome_fasta, ch_genome_fai ) From ec080027ed3dd0248e6484824b7448a07dedaa76 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 14 Aug 2023 10:51:50 +0000 Subject: [PATCH 038/387] Update changelog --- CHANGELOG.md | 3 +++ 1 file changed, 3 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index c89755f3..467e2e05 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -12,6 +12,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Changed` - Tiddit updated to v3.6.1 [#385](https://github.com/nf-core/raredisease/pull/385) +- Installed the nf-core version of the sentieon/bwamemindex module [#397](https://github.com/nf-core/raredisease/pull/397) +- Installed the nf-core version of the sentieon/bwamem module [#398](https://github.com/nf-core/raredisease/pull/398) +- Installed the nf-core version of the sentieon/readwriter module [#399](https://github.com/nf-core/raredisease/pull/399) ### `Fixed` From 719f56e65e3fa832898dc8f6bbe08b9b1fb36c52 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 14 Aug 2023 11:39:41 +0000 Subject: [PATCH 039/387] Replace local version of SENTIEON_DATAMETRICS with nf-core version --- modules.json | 5 ++ .../sentieon/datametrics/main.nf} | 29 +++++-- modules/nf-core/sentieon/datametrics/meta.yml | 84 +++++++++++++++++++ .../local/alignment/align_sentieon.nf | 2 +- 4 files changed, 114 insertions(+), 6 deletions(-) rename modules/{local/sentieon/datametrics.nf => nf-core/sentieon/datametrics/main.nf} (54%) create mode 100644 modules/nf-core/sentieon/datametrics/meta.yml diff --git a/modules.json b/modules.json index 5f9df891..2974defc 100644 --- a/modules.json +++ b/modules.json @@ -350,6 +350,11 @@ "git_sha": "b9172e8c26a3db5009f7872654c44587e254f094", "installed_by": ["modules"] }, + "sentieon/datametrics": { + "branch": "master", + "git_sha": "127edadc279e19da093fdd513926c6cdee82c306", + "installed_by": ["modules"] + }, "sentieon/readwriter": { "branch": "master", "git_sha": "b28e4dde755117e8dab5d6e85e292f145b8b53c3", diff --git a/modules/local/sentieon/datametrics.nf b/modules/nf-core/sentieon/datametrics/main.nf similarity index 54% rename from modules/local/sentieon/datametrics.nf rename to modules/nf-core/sentieon/datametrics/main.nf index 37ca6312..831a8f68 100644 --- a/modules/local/sentieon/datametrics.nf +++ b/modules/nf-core/sentieon/datametrics/main.nf @@ -1,10 +1,12 @@ process SENTIEON_DATAMETRICS { tag "$meta.id" - label 'process_high' + label 'process_medium' label 'sentieon' secret 'SENTIEON_LICENSE_BASE64' + container 'nf-core/sentieon:202112.06' + input: tuple val(meta), path(bam), path(bai) tuple val(meta2), path(fasta) @@ -23,13 +25,30 @@ process SENTIEON_DATAMETRICS { task.ext.when == null || task.ext.when script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + } def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def input = bam.sort().collect{"-i $it"}.join(' ') + def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: '' + def sentieon_auth_data_base64 = task.ext.sentieon_auth_data_base64 ?: '' """ - if [ \${SENTIEON_LICENSE_BASE64:-"unset"} != "unset" ]; then - echo "Initializing SENTIEON_LICENSE env variable" - source sentieon_init.sh SENTIEON_LICENSE_BASE64 + if [ "\${#SENTIEON_LICENSE_BASE64}" -lt "1500" ]; then # If the string SENTIEON_LICENSE_BASE64 is short, then it is an encrypted url. + export SENTIEON_LICENSE=\$(echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d) + else # Localhost license file + # The license file is stored as a nextflow variable like, for instance, this: + # nextflow secrets set SENTIEON_LICENSE_BASE64 \$(cat | base64 -w 0) + export SENTIEON_LICENSE=\$(mktemp) + echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d > \$SENTIEON_LICENSE + fi + + if [ ${sentieon_auth_mech_base64} ] && [ ${sentieon_auth_data_base64} ]; then + # If sentieon_auth_mech_base64 and sentieon_auth_data_base64 are non-empty strings, then Sentieon is mostly likely being run with some test-license. + export SENTIEON_AUTH_MECH=\$(echo -n "${sentieon_auth_mech_base64}" | base64 -d) + export SENTIEON_AUTH_DATA=\$(echo -n "${sentieon_auth_data_base64}" | base64 -d) + echo "Decoded and exported Sentieon test-license system environment variables" fi sentieon \\ @@ -51,7 +70,7 @@ process SENTIEON_DATAMETRICS { """ stub: - def prefix = task.ext.prefix ?: "${meta.id}" + def prefix = task.ext.prefix ?: "${meta.id}" """ touch ${prefix}_mq_metrics.txt touch ${prefix}_qd_metrics.txt diff --git a/modules/nf-core/sentieon/datametrics/meta.yml b/modules/nf-core/sentieon/datametrics/meta.yml new file mode 100644 index 00000000..bac58b9a --- /dev/null +++ b/modules/nf-core/sentieon/datametrics/meta.yml @@ -0,0 +1,84 @@ +name: "sentieon_datametrics" +description: Collects multiple quality metrics from a bam file +keywords: + - metrics + - bam + - sentieon +tools: + - sentieon: + description: | + Sentieon® provides complete solutions for secondary DNA/RNA analysis for a variety of sequencing platforms, including short and long reads. + Our software improves upon BWA, STAR, Minimap2, GATK, HaplotypeCaller, Mutect, and Mutect2 based pipelines and is deployable on any generic-CPU-based computing system. + homepage: https://www.sentieon.com/ + documentation: https://www.sentieon.com/ + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - meta2: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - meta3: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - bam: + type: file + description: Sorted BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" + - bai: + type: file + description: Index of th sorted BAM/CRAM/SAM file + pattern: "*.{bai,crai,sai}" + - fasta: + type: file + description: Genome fasta file + pattern: "*.{fa,fasta}" + - fai: + type: file + description: Index of the genome fasta file + pattern: "*.fai" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - mq_metrics: + type: file + description: File containing the information about mean base quality score for each sequencing cycle + pattern: "*.txt" + - qd_metrics: + type: file + description: File containing the information about number of bases with a specific base quality score + pattern: "*.txt" + - gc_summary: + type: file + description: File containing the information about GC bias in the reference and the sample + pattern: "*.txt" + - gc_metrics: + type: file + description: File containing the information about GC bias in the reference and the sample + pattern: "*.txt" + - aln_metrics: + type: file + description: File containing the statistics about the alignment of the reads + pattern: "*.txt" + - is_metrics: + type: file + description: File containing the information about statistical distribution of insert sizes + pattern: "*.txt" + +authors: + - "@ramprasadn" diff --git a/subworkflows/local/alignment/align_sentieon.nf b/subworkflows/local/alignment/align_sentieon.nf index 25673f2e..8cbc122b 100644 --- a/subworkflows/local/alignment/align_sentieon.nf +++ b/subworkflows/local/alignment/align_sentieon.nf @@ -3,7 +3,7 @@ // include { SENTIEON_BWAMEM } from '../../../modules/nf-core/sentieon/bwamem/main' -include { SENTIEON_DATAMETRICS } from '../../../modules/local/sentieon/datametrics' +include { SENTIEON_DATAMETRICS } from '../../../modules/nf-core/sentieon/datametrics/main' include { SENTIEON_LOCUSCOLLECTOR } from '../../../modules/local/sentieon/locuscollector' include { SENTIEON_DEDUP } from '../../../modules/local/sentieon/dedup' include { SENTIEON_BQSR } from '../../../modules/local/sentieon/bqsr' From ee1206a079de270fd76e018cc17d6e572ba73132 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 14 Aug 2023 11:59:48 +0000 Subject: [PATCH 040/387] Update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 467e2e05..4975544f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -15,6 +15,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Installed the nf-core version of the sentieon/bwamemindex module [#397](https://github.com/nf-core/raredisease/pull/397) - Installed the nf-core version of the sentieon/bwamem module [#398](https://github.com/nf-core/raredisease/pull/398) - Installed the nf-core version of the sentieon/readwriter module [#399](https://github.com/nf-core/raredisease/pull/399) +- Installed the nf-core version of the sentieon/datametrics module [#400](https://github.com/nf-core/raredisease/pull/400) ### `Fixed` From 864e2e9d7a94d67eb7090662262957012812e8b6 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 14 Aug 2023 12:38:00 +0000 Subject: [PATCH 041/387] Replace local version of sentieon-locuscollector and sentieon-dedup with nf-core versions --- conf/modules/align_sentieon.config | 4 -- modules.json | 5 ++ modules/local/sentieon/dedup.nf | 60 ------------------- modules/local/sentieon/locuscollector.nf | 52 ---------------- .../local/alignment/align_sentieon.nf | 10 +--- 5 files changed, 7 insertions(+), 124 deletions(-) delete mode 100644 modules/local/sentieon/dedup.nf delete mode 100644 modules/local/sentieon/locuscollector.nf diff --git a/conf/modules/align_sentieon.config b/conf/modules/align_sentieon.config index 5d89f69c..e3dd1cc2 100644 --- a/conf/modules/align_sentieon.config +++ b/conf/modules/align_sentieon.config @@ -34,10 +34,6 @@ process { ext.prefix = { "${meta.id}_merged" } } - withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_LOCUSCOLLECTOR' { - ext.prefix = { "${meta.id}_locuscollector" } - } - withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_DEDUP' { ext.args = { $params.rmdup ? "--rmdup" : '' } ext.prefix = { "${meta.id}_dedup" } diff --git a/modules.json b/modules.json index 2974defc..9e427d30 100644 --- a/modules.json +++ b/modules.json @@ -355,6 +355,11 @@ "git_sha": "127edadc279e19da093fdd513926c6cdee82c306", "installed_by": ["modules"] }, + "sentieon/dedup": { + "branch": "master", + "git_sha": "915a0b16ba3e40ef59e7b44843b3118e17a9c906", + "installed_by": ["modules"] + }, "sentieon/readwriter": { "branch": "master", "git_sha": "b28e4dde755117e8dab5d6e85e292f145b8b53c3", diff --git a/modules/local/sentieon/dedup.nf b/modules/local/sentieon/dedup.nf deleted file mode 100644 index bb738985..00000000 --- a/modules/local/sentieon/dedup.nf +++ /dev/null @@ -1,60 +0,0 @@ -process SENTIEON_DEDUP { - tag "$meta.id" - label 'process_high' - label 'sentieon' - - secret 'SENTIEON_LICENSE_BASE64' - - input: - tuple val(meta), path(bam), path(bai), path(score), path(score_idx) - tuple val(meta2), path(fasta) - tuple val(meta3), path(fai) - - output: - tuple val(meta), path('*.bam') , emit: bam - tuple val(meta), path('*.bam.bai') , emit: bai - tuple val(meta), path('*_metrics.txt'), emit: metrics_dedup - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def input = bam.sort().collect{"-i $it"}.join(' ') - """ - if [ \${SENTIEON_LICENSE_BASE64:-"unset"} != "unset" ]; then - echo "Initializing SENTIEON_LICENSE env variable" - source sentieon_init.sh SENTIEON_LICENSE_BASE64 - fi - - sentieon \\ - driver \\ - -t $task.cpus \\ - $input \\ - $args \\ - --algo Dedup \\ - --score_info $score \\ - --metrics ${prefix}_metrics.txt \\ - ${prefix}.bam - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.bam - touch ${prefix}.bam.bai - touch ${prefix}_metrics.txt - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - END_VERSIONS - """ -} diff --git a/modules/local/sentieon/locuscollector.nf b/modules/local/sentieon/locuscollector.nf deleted file mode 100644 index 9335b0ec..00000000 --- a/modules/local/sentieon/locuscollector.nf +++ /dev/null @@ -1,52 +0,0 @@ -process SENTIEON_LOCUSCOLLECTOR { - tag "$meta.id" - label 'process_high' - label 'sentieon' - - secret 'SENTIEON_LICENSE_BASE64' - - input: - tuple val(meta), path(bam), path(bai) - - output: - tuple val(meta), path('*txt.gz') , emit: score , optional: true - tuple val(meta), path('*txt.gz.tbi'), emit: score_idx, optional: true - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def input = bam.sort().collect{"-i $it"}.join(' ') - def prefix = task.ext.prefix ? "${task.ext.prefix}.txt.gz" : "${meta.id}.txt.gz" - """ - if [ \${SENTIEON_LICENSE_BASE64:-"unset"} != "unset" ]; then - echo "Initializing SENTIEON_LICENSE env variable" - source sentieon_init.sh SENTIEON_LICENSE_BASE64 - fi - - sentieon \\ - driver \\ - -t $task.cpus \\ - $input \\ - --algo LocusCollector \\ - --fun score_info $prefix - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ? "${task.ext.prefix}.txt.gz" : "${meta.id}.txt.gz" - """ - touch ${prefix}.txt.gz - touch ${prefix}.txt.gz.tbi - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - END_VERSIONS - """ -} diff --git a/subworkflows/local/alignment/align_sentieon.nf b/subworkflows/local/alignment/align_sentieon.nf index 8cbc122b..041255b2 100644 --- a/subworkflows/local/alignment/align_sentieon.nf +++ b/subworkflows/local/alignment/align_sentieon.nf @@ -5,6 +5,7 @@ include { SENTIEON_BWAMEM } from '../../../modules/nf-core/sentieon/bwamem/main' include { SENTIEON_DATAMETRICS } from '../../../modules/nf-core/sentieon/datametrics/main' include { SENTIEON_LOCUSCOLLECTOR } from '../../../modules/local/sentieon/locuscollector' +include { SENTIEON_DEDUP } from '../../../modules/nf-core/sentieon/dedup/main' include { SENTIEON_DEDUP } from '../../../modules/local/sentieon/dedup' include { SENTIEON_BQSR } from '../../../modules/local/sentieon/bqsr' include { SENTIEON_READWRITER } from '../../../modules/nf-core/sentieon/readwriter/main' @@ -45,14 +46,7 @@ workflow ALIGN_SENTIEON { SENTIEON_DATAMETRICS (ch_bam_bai, ch_genome_fasta, ch_genome_fai ) - SENTIEON_LOCUSCOLLECTOR ( ch_bam_bai ) - - ch_bam_bai - .join(SENTIEON_LOCUSCOLLECTOR.out.score, failOnMismatch:true, failOnDuplicate:true) - .join(SENTIEON_LOCUSCOLLECTOR.out.score_idx, failOnMismatch:true, failOnDuplicate:true) - .set { ch_bam_bai_score } - - SENTIEON_DEDUP ( ch_bam_bai_score, ch_genome_fasta, ch_genome_fai ) + SENTIEON_DEDUP ( ch_bam_bai, ch_genome_fasta, ch_genome_fai ) if (params.variant_caller == "sentieon") { SENTIEON_DEDUP.out.bam From e887f5143ca9a005fc8abd08cdbe4e033b08ccc3 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 14 Aug 2023 12:41:46 +0000 Subject: [PATCH 042/387] The new modules --- modules/nf-core/sentieon/dedup/main.nf | 86 ++++++++++++++++++++++++ modules/nf-core/sentieon/dedup/meta.yml | 88 +++++++++++++++++++++++++ 2 files changed, 174 insertions(+) create mode 100644 modules/nf-core/sentieon/dedup/main.nf create mode 100644 modules/nf-core/sentieon/dedup/meta.yml diff --git a/modules/nf-core/sentieon/dedup/main.nf b/modules/nf-core/sentieon/dedup/main.nf new file mode 100644 index 00000000..c83d5e55 --- /dev/null +++ b/modules/nf-core/sentieon/dedup/main.nf @@ -0,0 +1,86 @@ +process SENTIEON_DEDUP { + tag "$meta.id" + label 'process_medium' + label 'sentieon' + + secret 'SENTIEON_LICENSE_BASE64' + + container 'nf-core/sentieon:202112.06' + + input: + tuple val(meta), path(bam), path(bai) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fasta_fai) + + output: + tuple val(meta), path("*.cram") , emit: cram, optional: true + tuple val(meta), path("*.crai") , emit: crai, optional: true + tuple val(meta), path("*.bam") , emit: bam , optional: true + tuple val(meta), path("*.bai") , emit: bai + tuple val(meta), path("*.score") , emit: score + tuple val(meta), path("*.metrics") , emit: metrics + tuple val(meta), path("*.metrics.multiqc.tsv"), emit: metrics_multiqc_tsv + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + } + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def args3 = task.ext.args3 ?: '' + def args4 = task.ext.args4 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def suffix = task.ext.suffix ?: ".cram" // The suffix should be either ".cram" or ".bam". + def metrics = task.ext.metrics ?: "${prefix}${suffix}.metrics" + def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: '' + def sentieon_auth_data_base64 = task.ext.sentieon_auth_data_base64 ?: '' + def input_list = bam.collect{"-i $it"}.join(' ') + + """ + if [ "\${#SENTIEON_LICENSE_BASE64}" -lt "1500" ]; then # If the string SENTIEON_LICENSE_BASE64 is short, then it is an encrypted url. + export SENTIEON_LICENSE=\$(echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d) + else # Localhost license file + # The license file is stored as a nextflow variable like, for instance, this: + # nextflow secrets set SENTIEON_LICENSE_BASE64 \$(cat | base64 -w 0) + export SENTIEON_LICENSE=\$(mktemp) + echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d > \$SENTIEON_LICENSE + fi + + if [ ${sentieon_auth_mech_base64} ] && [ ${sentieon_auth_data_base64} ]; then + # If sentieon_auth_mech_base64 and sentieon_auth_data_base64 are non-empty strings, then Sentieon is mostly likely being run with some test-license. + export SENTIEON_AUTH_MECH=\$(echo -n "${sentieon_auth_mech_base64}" | base64 -d) + export SENTIEON_AUTH_DATA=\$(echo -n "${sentieon_auth_data_base64}" | base64 -d) + echo "Decoded and exported Sentieon test-license system environment variables" + fi + + sentieon driver $args $input_list -r ${fasta} --algo LocusCollector $args2 --fun score_info ${prefix}.score + sentieon driver $args3 -t $task.cpus $input_list -r ${fasta} --algo Dedup $args4 --score_info ${prefix}.score --metrics ${metrics} ${prefix}${suffix} + # This following tsv-file is produced in order to get a proper tsv-file with Dedup-metrics for importing in MultiQC as "custom content". + # It should be removed once MultiQC has a module for displaying Dedup-metrics. + head -3 ${metrics} > ${metrics}.multiqc.tsv + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.cram + touch ${prefix}.cram.crai + touch ${prefix}.metrics + touch ${prefix}.score + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/sentieon/dedup/meta.yml b/modules/nf-core/sentieon/dedup/meta.yml new file mode 100644 index 00000000..ec0565d9 --- /dev/null +++ b/modules/nf-core/sentieon/dedup/meta.yml @@ -0,0 +1,88 @@ +name: sentieon_dedup +description: Runs the sentieon tool LocusCollector followed by Dedup. LocusCollector collects read information that is used by Dedup which in turn marks or removes duplicate reads. +keywords: + - mem + - dedup + - map + - bam + - cram + - sentieon +tools: + - sentieon: + description: | + Sentieon® provides complete solutions for secondary DNA/RNA analysis for a variety of sequencing platforms, including short and long reads. + Our software improves upon BWA, STAR, Minimap2, GATK, HaplotypeCaller, Mutect, and Mutect2 based pipelines and is deployable on any generic-CPU-based computing system. + homepage: https://www.sentieon.com/ + documentation: https://www.sentieon.com/ +input: + - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file. + pattern: "*.bam" + - bai: + type: file + description: BAI file + pattern: "*.bai" + - meta2: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - fasta: + type: file + description: Genome fasta file + pattern: "*.{fa,fasta}" + - meta3: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - fasta_fai: + type: file + description: The index of the FASTA reference. + pattern: "*.fai" +output: + - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - cram: + type: file + description: CRAM file + pattern: "*.cram" + - crai: + type: file + description: CRAM index file + pattern: "*.crai" + - bam: + type: file + description: BAM file. + pattern: "*.bam" + - bai: + type: file + description: BAI file + pattern: "*.bai" + - score: + type: file + description: The score file indicates which reads LocusCollector finds are likely duplicates. + pattern: "*.score" + - metrics: + type: file + description: Output file containing Dedup metrics incl. histogram data. + pattern: "*.metrics" + - metrics_multiqc_tsv: + type: file + description: Output tsv-file containing Dedup metrics excl. histogram data. + pattern: "*.metrics.multiqc.tsv" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@asp8200" From 08a86ec2a2783b8771b3de6a05543e2786281765 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 14 Aug 2023 12:42:08 +0000 Subject: [PATCH 043/387] Remove includes of old modules --- subworkflows/local/alignment/align_sentieon.nf | 2 -- 1 file changed, 2 deletions(-) diff --git a/subworkflows/local/alignment/align_sentieon.nf b/subworkflows/local/alignment/align_sentieon.nf index 041255b2..a5b09ccd 100644 --- a/subworkflows/local/alignment/align_sentieon.nf +++ b/subworkflows/local/alignment/align_sentieon.nf @@ -4,9 +4,7 @@ include { SENTIEON_BWAMEM } from '../../../modules/nf-core/sentieon/bwamem/main' include { SENTIEON_DATAMETRICS } from '../../../modules/nf-core/sentieon/datametrics/main' -include { SENTIEON_LOCUSCOLLECTOR } from '../../../modules/local/sentieon/locuscollector' include { SENTIEON_DEDUP } from '../../../modules/nf-core/sentieon/dedup/main' -include { SENTIEON_DEDUP } from '../../../modules/local/sentieon/dedup' include { SENTIEON_BQSR } from '../../../modules/local/sentieon/bqsr' include { SENTIEON_READWRITER } from '../../../modules/nf-core/sentieon/readwriter/main' workflow ALIGN_SENTIEON { From 64b7a8dc5239f7d4beeb2112e42ed0ea181d75c3 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 14 Aug 2023 13:53:16 +0000 Subject: [PATCH 044/387] Delete legacy reference to SENTIEON_LOCUSCOLLECTOR.out.versions --- subworkflows/local/alignment/align_sentieon.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/subworkflows/local/alignment/align_sentieon.nf b/subworkflows/local/alignment/align_sentieon.nf index a5b09ccd..b7387a44 100644 --- a/subworkflows/local/alignment/align_sentieon.nf +++ b/subworkflows/local/alignment/align_sentieon.nf @@ -59,7 +59,6 @@ workflow ALIGN_SENTIEON { ch_versions = ch_versions.mix(SENTIEON_BWAMEM.out.versions.first()) ch_versions = ch_versions.mix(SENTIEON_DATAMETRICS.out.versions.first()) - ch_versions = ch_versions.mix(SENTIEON_LOCUSCOLLECTOR.out.versions.first()) ch_versions = ch_versions.mix(SENTIEON_DEDUP.out.versions.first()) emit: From 5e7fce73bc2a9b6f4b7a226fef89122ee4d93654 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 14 Aug 2023 14:32:49 +0000 Subject: [PATCH 045/387] Update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 4975544f..487d77c1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -16,6 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Installed the nf-core version of the sentieon/bwamem module [#398](https://github.com/nf-core/raredisease/pull/398) - Installed the nf-core version of the sentieon/readwriter module [#399](https://github.com/nf-core/raredisease/pull/399) - Installed the nf-core version of the sentieon/datametrics module [#400](https://github.com/nf-core/raredisease/pull/400) +- Installed the nf-core version of the sentieon/dedup module. The dedup module also contains a call to Sentieon's LocusCollector [#401](https://github.com/nf-core/raredisease/pull/401) ### `Fixed` From d972f6eae1c8f1e3f35b7cc07ed9ea80f7a1e593 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 14 Aug 2023 16:58:42 +0200 Subject: [PATCH 046/387] update mosdepth --- modules.json | 2 +- modules/nf-core/mosdepth/main.nf | 4 ++-- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/modules.json b/modules.json index 5297fca0..47ed9c2b 100644 --- a/modules.json +++ b/modules.json @@ -247,7 +247,7 @@ }, "mosdepth": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "ebb27711cd5f4de921244bfa81c676504072d31c", "installed_by": ["modules"] }, "multiqc": { diff --git a/modules/nf-core/mosdepth/main.nf b/modules/nf-core/mosdepth/main.nf index c17e4e65..74db3a27 100644 --- a/modules/nf-core/mosdepth/main.nf +++ b/modules/nf-core/mosdepth/main.nf @@ -35,10 +35,10 @@ process MOSDEPTH { def reference = fasta ? "--fasta ${fasta}" : "" def interval = bed ? "--by ${bed}" : "" if (bed && args.contains("--by")) { - exit 1, "'--by' can only be specified once when running mosdepth! Either remove input BED file definition or remove '--by' from 'ext.args' definition" + error "'--by' can only be specified once when running mosdepth! Either remove input BED file definition or remove '--by' from 'ext.args' definition" } if (!bed && args.contains("--thresholds")) { - exit 1, "'--thresholds' can only be specified in conjunction with '--by'" + error "'--thresholds' can only be specified in conjunction with '--by'" } """ From 6eb3284fc8eb232dfcaf920b9a49acd58e952469 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 14 Aug 2023 16:28:13 +0000 Subject: [PATCH 047/387] Remove sentieon BQSR --- conf/modules/align_sentieon.config | 3 - modules/local/sentieon/bqsr.nf | 88 ------------------- .../local/alignment/align_sentieon.nf | 20 ----- 3 files changed, 111 deletions(-) delete mode 100644 modules/local/sentieon/bqsr.nf diff --git a/conf/modules/align_sentieon.config b/conf/modules/align_sentieon.config index e3dd1cc2..b39bad3c 100644 --- a/conf/modules/align_sentieon.config +++ b/conf/modules/align_sentieon.config @@ -45,7 +45,4 @@ process { ] } - withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_BQSR' { - ext.prefix = { "${meta.id}_bqsr" } - } } diff --git a/modules/local/sentieon/bqsr.nf b/modules/local/sentieon/bqsr.nf deleted file mode 100644 index 390108e1..00000000 --- a/modules/local/sentieon/bqsr.nf +++ /dev/null @@ -1,88 +0,0 @@ -process SENTIEON_BQSR { - tag "$meta.id" - label 'process_high' - label 'sentieon' - - secret 'SENTIEON_LICENSE_BASE64' - - input: - tuple val(meta), path(bam), path(bai) - tuple val(meta2), path(fasta) - tuple val(meta3), path(fai) - tuple val(meta4), path(known_dbsnp) - tuple val(meta5), path(known_dbsnp_tbi) - - output: - tuple val(meta), path('*.bam') , emit: bam - tuple val(meta), path('*.bam.bai') , emit: bai - tuple val(meta), path('*.table') , emit: recal_pre - tuple val(meta), path('*.table_post'), emit: recal_post - tuple val(meta), path('*.csv') , emit: recal_csv - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - def args3 = task.ext.args3 ?: '' - def dbsnp = known_dbsnp ? "-k $known_dbsnp" : '' - def prefix = task.ext.prefix ?: "${meta.id}" - def input = bam.sort().collect{"-i $it"}.join(' ') - """ - if [ \${SENTIEON_LICENSE_BASE64:-"unset"} != "unset" ]; then - echo "Initializing SENTIEON_LICENSE env variable" - source sentieon_init.sh SENTIEON_LICENSE_BASE64 - fi - - sentieon driver \\ - -t ${task.cpus} \\ - -r $fasta \\ - $args \\ - $input \\ - --algo QualCal \\ - $dbsnp \\ - ${prefix}.table - - sentieon driver \\ - -t ${task.cpus} \\ - -r $fasta \\ - $args2 \\ - $input \\ - -q ${prefix}.table \\ - --algo QualCal \\ - $dbsnp \\ - ${prefix}.table_post \\ - --algo ReadWriter ${prefix}.bam - - sentieon driver \\ - -t ${task.cpus} \\ - $args3 \\ - --algo QualCal \\ - --plot \\ - --before ${prefix}.table \\ - --after ${prefix}.table_post \\ - ${prefix}.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.bam - touch ${prefix}.bam.bai - touch ${prefix}.table - touch ${prefix}.table_post - touch ${prefix}.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - END_VERSIONS - """ -} diff --git a/subworkflows/local/alignment/align_sentieon.nf b/subworkflows/local/alignment/align_sentieon.nf index b7387a44..86447e81 100644 --- a/subworkflows/local/alignment/align_sentieon.nf +++ b/subworkflows/local/alignment/align_sentieon.nf @@ -5,7 +5,6 @@ include { SENTIEON_BWAMEM } from '../../../modules/nf-core/sentieon/bwamem/main' include { SENTIEON_DATAMETRICS } from '../../../modules/nf-core/sentieon/datametrics/main' include { SENTIEON_DEDUP } from '../../../modules/nf-core/sentieon/dedup/main' -include { SENTIEON_BQSR } from '../../../modules/local/sentieon/bqsr' include { SENTIEON_READWRITER } from '../../../modules/nf-core/sentieon/readwriter/main' workflow ALIGN_SENTIEON { take: @@ -13,15 +12,10 @@ workflow ALIGN_SENTIEON { ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] ch_bwa_index // channel: [mandatory] [ val(meta), path(bwa_index) ] - ch_known_dbsnp // channel: [optional] [ path(known_dbsnp) ] - ch_known_dbsnp_tbi // channel: [optional] [ path(known_dbsnp_tbi) ] val_platform // string: [mandatory] default: illumina main: ch_versions = Channel.empty() - ch_bqsr_bam = Channel.empty() - ch_bqsr_bai = Channel.empty() - ch_bqsr_csv = Channel.empty() SENTIEON_BWAMEM ( ch_reads_input, ch_bwa_index, ch_genome_fasta.map{ meta, fasta -> fasta }, ch_genome_fai.map{ meta, fai -> fai }) @@ -46,17 +40,6 @@ workflow ALIGN_SENTIEON { SENTIEON_DEDUP ( ch_bam_bai, ch_genome_fasta, ch_genome_fai ) - if (params.variant_caller == "sentieon") { - SENTIEON_DEDUP.out.bam - .join(SENTIEON_DEDUP.out.bai, failOnMismatch:true, failOnDuplicate:true) - .set { ch_dedup_bam_bai } - SENTIEON_BQSR ( ch_dedup_bam_bai, ch_genome_fasta, ch_genome_fai, ch_known_dbsnp, ch_known_dbsnp_tbi ) - ch_bqsr_bam = SENTIEON_BQSR.out.bam - ch_bqsr_bai = SENTIEON_BQSR.out.bai - ch_bqsr_csv = SENTIEON_BQSR.out.recal_csv - ch_versions = ch_versions.mix(SENTIEON_BQSR.out.versions.first()) - } - ch_versions = ch_versions.mix(SENTIEON_BWAMEM.out.versions.first()) ch_versions = ch_versions.mix(SENTIEON_DATAMETRICS.out.versions.first()) ch_versions = ch_versions.mix(SENTIEON_DEDUP.out.versions.first()) @@ -64,9 +47,6 @@ workflow ALIGN_SENTIEON { emit: marked_bam = SENTIEON_DEDUP.out.bam // channel: [ val(meta), path(bam) ] marked_bai = SENTIEON_DEDUP.out.bai // channel: [ val(meta), path(bai) ] - recal_bam = ch_bqsr_bam.ifEmpty(null) // channel: [ val(meta), path(bam) ] - recal_bai = ch_bqsr_bai.ifEmpty(null) // channel: [ val(meta), path(bai) ] - recal_csv = ch_bqsr_csv.ifEmpty(null) // channel: [ val(meta), path(csv) ] mq_metrics = SENTIEON_DATAMETRICS.out.mq_metrics.ifEmpty(null) // channel: [ val(meta), path(mq_metrics) ] qd_metrics = SENTIEON_DATAMETRICS.out.qd_metrics.ifEmpty(null) // channel: [ val(meta), path(qd_metrics) ] gc_metrics = SENTIEON_DATAMETRICS.out.gc_metrics.ifEmpty(null) // channel: [ val(meta), path(gc_metrics) ] From ef8f621f2580f1acea51463cd685535a9b183d6c Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 14 Aug 2023 16:46:18 +0000 Subject: [PATCH 048/387] Removing dbsnp as argument to ALIGN and ALIGN_SENTIEON --- subworkflows/local/align.nf | 4 ---- workflows/raredisease.nf | 2 -- 2 files changed, 6 deletions(-) diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index bab66216..3455c3b9 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -13,8 +13,6 @@ workflow ALIGN { ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] ch_bwa_index // channel: [mandatory] [ val(meta), path(index) ] ch_bwamem2_index // channel: [mandatory] [ val(meta), path(index) ] - ch_known_dbsnp // channel: [optional; used by sentieon] [ path(known_dbsnp) ] - ch_known_dbsnp_tbi // channel: [optional; used by sentieon] [ path(known_dbsnp_tbi) ] val_platform // string: [mandatory] illumina or a different technology main: @@ -33,8 +31,6 @@ workflow ALIGN { ch_genome_fasta, ch_genome_fai, ch_bwa_index, - ch_known_dbsnp, - ch_known_dbsnp_tbi, val_platform ) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index abd34e39..0704b202 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -300,8 +300,6 @@ workflow RAREDISEASE { ch_genome_fai, ch_genome_bwaindex, ch_genome_bwamem2index, - ch_dbsnp, - ch_dbsnp_tbi, params.platform ) .set { ch_mapped } From bf5e5ea7eebff673ceab5f693fb5502d9b2b2e08 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Mon, 14 Aug 2023 17:04:48 +0000 Subject: [PATCH 049/387] Update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 487d77c1..e7e1480a 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -17,6 +17,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Installed the nf-core version of the sentieon/readwriter module [#399](https://github.com/nf-core/raredisease/pull/399) - Installed the nf-core version of the sentieon/datametrics module [#400](https://github.com/nf-core/raredisease/pull/400) - Installed the nf-core version of the sentieon/dedup module. The dedup module also contains a call to Sentieon's LocusCollector [#401](https://github.com/nf-core/raredisease/pull/401) +- Removing Sentieon-based BQSR. Recent Illumina sequencers tend to provide well-calibrated BQs, so BQSR may not provide much benefit [#402](https://github.com/nf-core/raredisease/pull/402) ### `Fixed` From 004a003d440774448275055b3933aecdc56856e5 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Tue, 15 Aug 2023 07:53:28 +0000 Subject: [PATCH 050/387] Replace local version of SENTIEON_DNAMODELAPPLY with nf-core version --- modules.json | 5 ++ modules/local/sentieon/dnamodelapply.nf | 50 ------------ .../nf-core/sentieon/dnamodelapply/main.nf | 81 +++++++++++++++++++ .../nf-core/sentieon/dnamodelapply/meta.yml | 78 ++++++++++++++++++ .../variant_calling/call_snv_sentieon.nf | 4 +- 5 files changed, 166 insertions(+), 52 deletions(-) delete mode 100644 modules/local/sentieon/dnamodelapply.nf create mode 100644 modules/nf-core/sentieon/dnamodelapply/main.nf create mode 100644 modules/nf-core/sentieon/dnamodelapply/meta.yml diff --git a/modules.json b/modules.json index 9e427d30..b4e2c848 100644 --- a/modules.json +++ b/modules.json @@ -360,6 +360,11 @@ "git_sha": "915a0b16ba3e40ef59e7b44843b3118e17a9c906", "installed_by": ["modules"] }, + "sentieon/dnamodelapply": { + "branch": "master", + "git_sha": "43ef68091a1188fd8dc4c03f9341b556803c7514", + "installed_by": ["modules"] + }, "sentieon/readwriter": { "branch": "master", "git_sha": "b28e4dde755117e8dab5d6e85e292f145b8b53c3", diff --git a/modules/local/sentieon/dnamodelapply.nf b/modules/local/sentieon/dnamodelapply.nf deleted file mode 100644 index 32582b2c..00000000 --- a/modules/local/sentieon/dnamodelapply.nf +++ /dev/null @@ -1,50 +0,0 @@ -process SENTIEON_DNAMODELAPPLY { - tag "$meta.id" - label 'process_high' - label 'sentieon' - - input: - tuple val(meta), path(vcf), path(vcf_idx) - tuple val(meta2), path(fasta) - tuple val(meta3), path(fai) - path ml_model - - output: - tuple val(meta), path("*.vcf.gz") , emit: vcf - tuple val(meta), path("*.vcf.gz.tbi") , emit: index - tuple val(meta), path("*.vcf.gz"), path("*.vcf.gz.tbi"), emit: vcf_index - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def prefix = task.ext.prefix ?: "${meta.id}" - - """ - sentieon driver \\ - -t $task.cpus \\ - -r $fasta \\ - --algo DNAModelApply \\ - --model $ml_model \\ - -v $vcf \\ - ${prefix}.vcf.gz - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.vcf.gz - touch ${prefix}.vcf.gz.tbi - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g" ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/sentieon/dnamodelapply/main.nf b/modules/nf-core/sentieon/dnamodelapply/main.nf new file mode 100644 index 00000000..3fe9a28f --- /dev/null +++ b/modules/nf-core/sentieon/dnamodelapply/main.nf @@ -0,0 +1,81 @@ +process SENTIEON_DNAMODELAPPLY { + tag "$meta.id" + label 'process_high' + label 'sentieon' + + secret 'SENTIEON_LICENSE_BASE64' + + container 'nf-core/sentieon:202112.06' + + input: + tuple val(meta), path(vcf), path(idx) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fai) + tuple val(meta4), path(ml_model) + + output: + tuple val(meta), path("*.vcf.gz") , emit: vcf + tuple val(meta), path("*.vcf.gz.tbi"), emit: index + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + } + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: '' + def sentieon_auth_data_base64 = task.ext.sentieon_auth_data_base64 ?: '' + + """ + if [ "\${#SENTIEON_LICENSE_BASE64}" -lt "1500" ]; then # If the string SENTIEON_LICENSE_BASE64 is short, then it is an encrypted url. + export SENTIEON_LICENSE=\$(echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d) + else # Localhost license file + # The license file is stored as a nextflow variable like, for instance, this: + # nextflow secrets set SENTIEON_LICENSE_BASE64 \$(cat | base64 -w 0) + export SENTIEON_LICENSE=\$(mktemp) + echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d > \$SENTIEON_LICENSE + fi + + if [ ${sentieon_auth_mech_base64} ] && [ ${sentieon_auth_data_base64} ]; then + # If sentieon_auth_mech_base64 and sentieon_auth_data_base64 are non-empty strings, then Sentieon is mostly likely being run with some test-license. + export SENTIEON_AUTH_MECH=\$(echo -n "${sentieon_auth_mech_base64}" | base64 -d) + export SENTIEON_AUTH_DATA=\$(echo -n "${sentieon_auth_data_base64}" | base64 -d) + echo "Decoded and exported Sentieon test-license system environment variables" + fi + + sentieon driver \\ + -t $task.cpus \\ + -r $fasta \\ + $args \\ + --algo DNAModelApply \\ + --model $ml_model \\ + -v $vcf \\ + ${prefix}.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + } + """ + touch ${prefix}.vcf.gz + touch ${prefix}.vcf.gz.tbi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g" ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/sentieon/dnamodelapply/meta.yml b/modules/nf-core/sentieon/dnamodelapply/meta.yml new file mode 100644 index 00000000..ec429bea --- /dev/null +++ b/modules/nf-core/sentieon/dnamodelapply/meta.yml @@ -0,0 +1,78 @@ +name: "sentieon_dnamodelapply" +description: modifies the input VCF file by adding the MLrejected FILTER to the variants +keywords: + - dnamodelapply + - vcf + - filter + - sentieon +tools: + - sentieon: + description: | + Sentieon® provides complete solutions for secondary DNA/RNA analysis for a variety of sequencing platforms, including short and long reads. + Our software improves upon BWA, STAR, Minimap2, GATK, HaplotypeCaller, Mutect, and Mutect2 based pipelines and is deployable on any generic-CPU-based computing system. + homepage: https://www.sentieon.com/ + documentation: https://www.sentieon.com/ + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. `[ id:'test' ]` + - meta3: + type: map + description: | + Groovy Map containing reference information + e.g. `[ id:'test' ]` + - meta4: + type: map + description: | + Groovy Map containing reference information + e.g. `[ id:'test' ]` + - vcf: + type: file + description: INPUT VCF file + pattern: "*.{vcf,vcf.gz}" + - idx: + type: file + description: Index of the input VCF file + pattern: "*.{tbi}" + - fasta: + type: file + description: Genome fasta file + pattern: "*.{fa,fasta}" + - fai: + type: file + description: Index of the genome fasta file + pattern: "*.fai" + - ml_model: + type: file + description: machine learning model file + pattern: "*.model" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: INPUT VCF file + pattern: "*.{vcf,vcf.gz}" + - index: + type: file + description: Index of the input VCF file + pattern: "*.{tbi}" + +authors: + - "@ramprasadn" diff --git a/subworkflows/local/variant_calling/call_snv_sentieon.nf b/subworkflows/local/variant_calling/call_snv_sentieon.nf index d1b8bbb2..bbcdbc19 100644 --- a/subworkflows/local/variant_calling/call_snv_sentieon.nf +++ b/subworkflows/local/variant_calling/call_snv_sentieon.nf @@ -3,7 +3,7 @@ // include { SENTIEON_DNASCOPE } from '../../../modules/local/sentieon/dnascope' -include { SENTIEON_DNAMODELAPPLY } from '../../../modules/local/sentieon/dnamodelapply' +include { SENTIEON_DNAMODELAPPLY } from '../../../modules/nf-core/sentieon/dnamodelapply/main' include { BCFTOOLS_MERGE } from '../../../modules/nf-core/bcftools/merge/main' include { BCFTOOLS_NORM as SPLIT_MULTIALLELICS_SEN } from '../../../modules/nf-core/bcftools/norm/main' include { BCFTOOLS_NORM as REMOVE_DUPLICATES_SEN } from '../../../modules/nf-core/bcftools/norm/main' @@ -28,7 +28,7 @@ workflow CALL_SNV_SENTIEON { SENTIEON_DNASCOPE ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_dbsnp, ch_dbsnp_index, ch_call_interval, ch_ml_model ) - SENTIEON_DNAMODELAPPLY ( SENTIEON_DNASCOPE.out.vcf_index, ch_genome_fasta, ch_genome_fai, ch_ml_model ) + SENTIEON_DNAMODELAPPLY ( SENTIEON_DNASCOPE.out.vcf_index, ch_genome_fasta, ch_genome_fai, [ [:], ch_ml_model ] ) BCF_FILTER_ONE (SENTIEON_DNAMODELAPPLY.out.vcf ) From c1600352d247ee10c0c08bb2bdb25d5401fa75e9 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Tue, 15 Aug 2023 08:22:44 +0000 Subject: [PATCH 051/387] Update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index e7e1480a..31781c81 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -18,6 +18,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Installed the nf-core version of the sentieon/datametrics module [#400](https://github.com/nf-core/raredisease/pull/400) - Installed the nf-core version of the sentieon/dedup module. The dedup module also contains a call to Sentieon's LocusCollector [#401](https://github.com/nf-core/raredisease/pull/401) - Removing Sentieon-based BQSR. Recent Illumina sequencers tend to provide well-calibrated BQs, so BQSR may not provide much benefit [#402](https://github.com/nf-core/raredisease/pull/402) +- Installed the nf-core version of the sentieon/dnamodelapply module [#403](https://github.com/nf-core/raredisease/pull/403) ### `Fixed` From 2640c83167e15a712e497b81fb835b8ccc4c63b8 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Tue, 15 Aug 2023 12:02:54 +0000 Subject: [PATCH 052/387] Replace local version of SENTIEON_WGSMETRICS with nf-core version --- modules.json | 5 ++ modules/local/sentieon/wgsmetricsalgo.nf | 57 -------------- modules/nf-core/sentieon/wgsmetrics/main.nf | 81 ++++++++++++++++++++ modules/nf-core/sentieon/wgsmetrics/meta.yml | 73 ++++++++++++++++++ subworkflows/local/qc_bam.nf | 8 +- 5 files changed, 163 insertions(+), 61 deletions(-) delete mode 100644 modules/local/sentieon/wgsmetricsalgo.nf create mode 100644 modules/nf-core/sentieon/wgsmetrics/main.nf create mode 100644 modules/nf-core/sentieon/wgsmetrics/meta.yml diff --git a/modules.json b/modules.json index b4e2c848..5e1764b9 100644 --- a/modules.json +++ b/modules.json @@ -370,6 +370,11 @@ "git_sha": "b28e4dde755117e8dab5d6e85e292f145b8b53c3", "installed_by": ["modules"] }, + "sentieon/wgsmetrics": { + "branch": "master", + "git_sha": "b1e9314b919f498e116bfc1417b7185185e99261", + "installed_by": ["modules"] + }, "smncopynumbercaller": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", diff --git a/modules/local/sentieon/wgsmetricsalgo.nf b/modules/local/sentieon/wgsmetricsalgo.nf deleted file mode 100644 index 3663947d..00000000 --- a/modules/local/sentieon/wgsmetricsalgo.nf +++ /dev/null @@ -1,57 +0,0 @@ -process SENTIEON_WGSMETRICSALGO { - tag "$meta.id" - label 'process_medium' - label 'sentieon' - - secret 'SENTIEON_LICENSE_BASE64' - - input: - tuple val(meta), path(bam), path(bai) - tuple val(meta2), path(fasta) - tuple val(meta3), path(fai) - path intervals_list - - output: - tuple val(meta), path('*.txt'), emit: wgs_metrics - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def input = bam.sort().collect{"-i $it"}.join(' ') - def prefix = task.ext.prefix ?: "${meta.id}" - def interval = intervals_list ? "--interval ${intervals_list}" : "" - """ - if [ \${SENTIEON_LICENSE_BASE64:-"unset"} != "unset" ]; then - echo "Initializing SENTIEON_LICENSE env variable" - source sentieon_init.sh SENTIEON_LICENSE_BASE64 - fi - - sentieon \\ - driver \\ - -t $task.cpus \\ - -r $fasta \\ - $input \\ - $interval \\ - $args \\ - --algo WgsMetricsAlgo ${prefix}.txt - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}_wgs_metrics.txt - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - END_VERSIONS - """ -} diff --git a/modules/nf-core/sentieon/wgsmetrics/main.nf b/modules/nf-core/sentieon/wgsmetrics/main.nf new file mode 100644 index 00000000..fd7fdae5 --- /dev/null +++ b/modules/nf-core/sentieon/wgsmetrics/main.nf @@ -0,0 +1,81 @@ +process SENTIEON_WGSMETRICS { + tag "$meta.id" + label 'process_medium' + label 'sentieon' + + secret 'SENTIEON_LICENSE_BASE64' + + container 'nf-core/sentieon:202112.06' + + input: + tuple val(meta), path(bam), path(bai) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fai) + tuple val(meta4), path(intervals_list) + + + output: + tuple val(meta), path('*.txt'), emit: wgs_metrics + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + } + def args = task.ext.args ?: '' + def input = bam.sort().collect{"-i $it"}.join(' ') + def prefix = task.ext.prefix ?: "${meta.id}" + def interval = intervals_list ? "--interval ${intervals_list}" : "" + def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: '' + def sentieon_auth_data_base64 = task.ext.sentieon_auth_data_base64 ?: '' + """ + if [ "\${#SENTIEON_LICENSE_BASE64}" -lt "1500" ]; then # If the string SENTIEON_LICENSE_BASE64 is short, then it is an encrypted url. + export SENTIEON_LICENSE=\$(echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d) + else # Localhost license file + # The license file is stored as a nextflow variable like, for instance, this: + # nextflow secrets set SENTIEON_LICENSE_BASE64 \$(cat | base64 -w 0) + export SENTIEON_LICENSE=\$(mktemp) + echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d > \$SENTIEON_LICENSE + fi + + if [ ${sentieon_auth_mech_base64} ] && [ ${sentieon_auth_data_base64} ]; then + # If sentieon_auth_mech_base64 and sentieon_auth_data_base64 are non-empty strings, then Sentieon is mostly likely being run with some test-license. + export SENTIEON_AUTH_MECH=\$(echo -n "${sentieon_auth_mech_base64}" | base64 -d) + export SENTIEON_AUTH_DATA=\$(echo -n "${sentieon_auth_data_base64}" | base64 -d) + echo "Decoded and exported Sentieon test-license system environment variables" + fi + + sentieon \\ + driver \\ + -t $task.cpus \\ + -r $fasta \\ + $input \\ + $interval \\ + $args \\ + --algo WgsMetricsAlgo ${prefix}.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") + END_VERSIONS + """ + + stub: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + } + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/sentieon/wgsmetrics/meta.yml b/modules/nf-core/sentieon/wgsmetrics/meta.yml new file mode 100644 index 00000000..09fb9eca --- /dev/null +++ b/modules/nf-core/sentieon/wgsmetrics/meta.yml @@ -0,0 +1,73 @@ +name: "sentieon_wgsmetrics" +description: Collects whole genome quality metrics from a bam file +keywords: + - metrics + - bam + - sentieon +tools: + - sentieon: + description: | + Sentieon® provides complete solutions for secondary DNA/RNA analysis for a variety of sequencing platforms, including short and long reads. + Our software improves upon BWA, STAR, Minimap2, GATK, HaplotypeCaller, Mutect, and Mutect2 based pipelines and is deployable on any generic-CPU-based computing system. + homepage: https://www.sentieon.com/ + documentation: https://www.sentieon.com/ + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. `[ id:'test' ]` + - meta3: + type: map + description: | + Groovy Map containing reference information + e.g. `[ id:'test' ]` + - meta4: + type: map + description: | + Groovy Map containing reference information + e.g. `[ id:'test' ]` + - bam: + type: file + description: Sorted BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" + - bai: + type: file + description: Index of th sorted BAM/CRAM/SAM file + pattern: "*.{bai,crai,sai}" + - fasta: + type: file + description: Genome fasta file + pattern: "*.{fa,fasta}" + - fai: + type: file + description: Index of the genome fasta file + pattern: "*.fai" + - interval_list: + type: file + description: bed or interval_list file containing interval in the reference that will be used in the analysis + pattern: "*.{bed,interval_list}" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - wgs_metrics: + type: file + description: File containing the information about mean base quality score for each sequencing cycle + pattern: "*.txt" + +authors: + - "@ramprasadn" diff --git a/subworkflows/local/qc_bam.nf b/subworkflows/local/qc_bam.nf index 06b55422..d593bac9 100644 --- a/subworkflows/local/qc_bam.nf +++ b/subworkflows/local/qc_bam.nf @@ -10,8 +10,8 @@ include { MOSDEPTH } from '../../m include { UCSC_WIGTOBIGWIG } from '../../modules/nf-core/ucsc/wigtobigwig/main' include { PICARD_COLLECTWGSMETRICS as PICARD_COLLECTWGSMETRICS } from '../../modules/nf-core/picard/collectwgsmetrics/main' include { PICARD_COLLECTWGSMETRICS as PICARD_COLLECTWGSMETRICS_Y } from '../../modules/nf-core/picard/collectwgsmetrics/main' -include { SENTIEON_WGSMETRICSALGO as SENTIEON_WGSMETRICS } from '../../modules/local/sentieon/wgsmetricsalgo' -include { SENTIEON_WGSMETRICSALGO as SENTIEON_WGSMETRICS_Y } from '../../modules/local/sentieon/wgsmetricsalgo' +include { SENTIEON_WGSMETRICSALGO as SENTIEON_WGSMETRICS } from '../../modules/nf-core/sentieon/wgsmetrics/main' +include { SENTIEON_WGSMETRICSALGO as SENTIEON_WGSMETRICS_Y } from '../../modules/nf-core/sentieon/wgsmetrics/main' workflow QC_BAM { @@ -52,8 +52,8 @@ workflow QC_BAM { PICARD_COLLECTWGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_wgs ) PICARD_COLLECTWGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_y ) - SENTIEON_WGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_wgs ) - SENTIEON_WGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_y ) + SENTIEON_WGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, [[:], ch_intervals_wgs] ) + SENTIEON_WGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, [[:], ch_intervals_y] ) ch_cov = Channel.empty().mix(PICARD_COLLECTWGSMETRICS.out.metrics, SENTIEON_WGSMETRICS.out.wgs_metrics) ch_cov_y = Channel.empty().mix(PICARD_COLLECTWGSMETRICS_Y.out.metrics, SENTIEON_WGSMETRICS_Y.out.wgs_metrics) From 50cd408ce015a7ad2088dd655c8f87382dbec154 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Tue, 15 Aug 2023 12:07:22 +0000 Subject: [PATCH 053/387] SENTIEON_WGSMETRICSALGO replaced by SENTIEON_WGSMETRICS --- subworkflows/local/qc_bam.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/qc_bam.nf b/subworkflows/local/qc_bam.nf index d593bac9..9519c2a1 100644 --- a/subworkflows/local/qc_bam.nf +++ b/subworkflows/local/qc_bam.nf @@ -10,8 +10,8 @@ include { MOSDEPTH } from '../../m include { UCSC_WIGTOBIGWIG } from '../../modules/nf-core/ucsc/wigtobigwig/main' include { PICARD_COLLECTWGSMETRICS as PICARD_COLLECTWGSMETRICS } from '../../modules/nf-core/picard/collectwgsmetrics/main' include { PICARD_COLLECTWGSMETRICS as PICARD_COLLECTWGSMETRICS_Y } from '../../modules/nf-core/picard/collectwgsmetrics/main' -include { SENTIEON_WGSMETRICSALGO as SENTIEON_WGSMETRICS } from '../../modules/nf-core/sentieon/wgsmetrics/main' -include { SENTIEON_WGSMETRICSALGO as SENTIEON_WGSMETRICS_Y } from '../../modules/nf-core/sentieon/wgsmetrics/main' +include { SENTIEON_WGSMETRICS as SENTIEON_WGSMETRICS } from '../../modules/nf-core/sentieon/wgsmetrics/main' +include { SENTIEON_WGSMETRICS as SENTIEON_WGSMETRICS_Y } from '../../modules/nf-core/sentieon/wgsmetrics/main' workflow QC_BAM { From d2e74922522b7790c36f4fdabb0261c9065dbed4 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Tue, 15 Aug 2023 12:19:00 +0000 Subject: [PATCH 054/387] Trying to fix input-channel with intervals for sentieon-wgsmetrics module --- subworkflows/local/qc_bam.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/qc_bam.nf b/subworkflows/local/qc_bam.nf index 9519c2a1..8308c221 100644 --- a/subworkflows/local/qc_bam.nf +++ b/subworkflows/local/qc_bam.nf @@ -52,8 +52,8 @@ workflow QC_BAM { PICARD_COLLECTWGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_wgs ) PICARD_COLLECTWGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_y ) - SENTIEON_WGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, [[:], ch_intervals_wgs] ) - SENTIEON_WGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, [[:], ch_intervals_y] ) + SENTIEON_WGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_wgs.map{ interval -> [[:] interval]} ) + SENTIEON_WGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_y.map{ interval -> [[:] interval]} ) ch_cov = Channel.empty().mix(PICARD_COLLECTWGSMETRICS.out.metrics, SENTIEON_WGSMETRICS.out.wgs_metrics) ch_cov_y = Channel.empty().mix(PICARD_COLLECTWGSMETRICS_Y.out.metrics, SENTIEON_WGSMETRICS_Y.out.wgs_metrics) From d8094c27bbec07c576946ef8606f9c32dac145c4 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Tue, 15 Aug 2023 12:21:36 +0000 Subject: [PATCH 055/387] Fixing typo --- subworkflows/local/qc_bam.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/qc_bam.nf b/subworkflows/local/qc_bam.nf index 8308c221..a86cd7ee 100644 --- a/subworkflows/local/qc_bam.nf +++ b/subworkflows/local/qc_bam.nf @@ -52,8 +52,8 @@ workflow QC_BAM { PICARD_COLLECTWGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_wgs ) PICARD_COLLECTWGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_y ) - SENTIEON_WGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_wgs.map{ interval -> [[:] interval]} ) - SENTIEON_WGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_y.map{ interval -> [[:] interval]} ) + SENTIEON_WGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_wgs.map{ interval -> [[:], interval]} ) + SENTIEON_WGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_y.map{ interval -> [[:], interval]} ) ch_cov = Channel.empty().mix(PICARD_COLLECTWGSMETRICS.out.metrics, SENTIEON_WGSMETRICS.out.wgs_metrics) ch_cov_y = Channel.empty().mix(PICARD_COLLECTWGSMETRICS_Y.out.metrics, SENTIEON_WGSMETRICS_Y.out.wgs_metrics) From 3c1291a606834627e8c3845c64043fd108db77a4 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Tue, 15 Aug 2023 12:59:58 +0000 Subject: [PATCH 056/387] Update changelog --- CHANGELOG.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 31781c81..2b548c69 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -19,6 +19,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Installed the nf-core version of the sentieon/dedup module. The dedup module also contains a call to Sentieon's LocusCollector [#401](https://github.com/nf-core/raredisease/pull/401) - Removing Sentieon-based BQSR. Recent Illumina sequencers tend to provide well-calibrated BQs, so BQSR may not provide much benefit [#402](https://github.com/nf-core/raredisease/pull/402) - Installed the nf-core version of the sentieon/dnamodelapply module [#403](https://github.com/nf-core/raredisease/pull/403) +- Installed the nf-core version of the sentieon/wgsmetricsalgo module [#404](https://github.com/nf-core/raredisease/pull/404) + ### `Fixed` From a9993518db2f98ebe3bccacc23c4b26731303202 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Tue, 15 Aug 2023 13:07:21 +0000 Subject: [PATCH 057/387] prettier --- CHANGELOG.md | 1 - 1 file changed, 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 2b548c69..244eda8d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -21,7 +21,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Installed the nf-core version of the sentieon/dnamodelapply module [#403](https://github.com/nf-core/raredisease/pull/403) - Installed the nf-core version of the sentieon/wgsmetricsalgo module [#404](https://github.com/nf-core/raredisease/pull/404) - ### `Fixed` - Invalid GATK4 container which caused incorrect singularity downloads with nf-core download [nf-core/modules #3668](https://github.com/nf-core/modules/issues/3668) From cc653d32fab318b59499c0c8750ada8f509f2730 Mon Sep 17 00:00:00 2001 From: Anders Sune Pedersen <37172585+asp8200@users.noreply.github.com> Date: Tue, 15 Aug 2023 15:11:44 +0200 Subject: [PATCH 058/387] Update subworkflows/local/qc_bam.nf Co-authored-by: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> --- subworkflows/local/qc_bam.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/qc_bam.nf b/subworkflows/local/qc_bam.nf index a86cd7ee..9331523e 100644 --- a/subworkflows/local/qc_bam.nf +++ b/subworkflows/local/qc_bam.nf @@ -10,7 +10,7 @@ include { MOSDEPTH } from '../../m include { UCSC_WIGTOBIGWIG } from '../../modules/nf-core/ucsc/wigtobigwig/main' include { PICARD_COLLECTWGSMETRICS as PICARD_COLLECTWGSMETRICS } from '../../modules/nf-core/picard/collectwgsmetrics/main' include { PICARD_COLLECTWGSMETRICS as PICARD_COLLECTWGSMETRICS_Y } from '../../modules/nf-core/picard/collectwgsmetrics/main' -include { SENTIEON_WGSMETRICS as SENTIEON_WGSMETRICS } from '../../modules/nf-core/sentieon/wgsmetrics/main' +include { SENTIEON_WGSMETRICS } from '../../modules/nf-core/sentieon/wgsmetrics/main' include { SENTIEON_WGSMETRICS as SENTIEON_WGSMETRICS_Y } from '../../modules/nf-core/sentieon/wgsmetrics/main' workflow QC_BAM { From dc9e4465c07e852a7201832dc7db7a1e5b547e14 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 16 Aug 2023 18:15:18 +0200 Subject: [PATCH 059/387] add filtervep --- modules.json | 7 ++++++- modules/nf-core/sentieon/bwamem/main.nf | 4 ++-- modules/nf-core/sentieon/bwamem/meta.yml | 10 ++++++++++ 3 files changed, 18 insertions(+), 3 deletions(-) diff --git a/modules.json b/modules.json index bd9dc135..ba35fbad 100644 --- a/modules.json +++ b/modules.json @@ -95,6 +95,11 @@ "git_sha": "39656f68219340420f03bd54a68e111c86e107e6", "installed_by": ["modules"] }, + "ensemblvep/filtervep": { + "branch": "master", + "git_sha": "29984d70aea47d06f0062a1785d76c357dd40ea9", + "installed_by": ["modules"] + }, "expansionhunter": { "branch": "master", "git_sha": "0260e5d22372eae434816d6970dedf3f5adc0053", @@ -352,7 +357,7 @@ }, "sentieon/bwamem": { "branch": "master", - "git_sha": "b9172e8c26a3db5009f7872654c44587e254f094", + "git_sha": "149b4746c6e16ef84f64db5bb245d5b9495fdc3f", "installed_by": ["modules"] }, "sentieon/datametrics": { diff --git a/modules/nf-core/sentieon/bwamem/main.nf b/modules/nf-core/sentieon/bwamem/main.nf index aeca2825..b58835fd 100644 --- a/modules/nf-core/sentieon/bwamem/main.nf +++ b/modules/nf-core/sentieon/bwamem/main.nf @@ -10,8 +10,8 @@ process SENTIEON_BWAMEM { input: tuple val(meta), path(reads) tuple val(meta2), path(index) - path(fasta) - path(fasta_fai) + tuple val(meta3), path(fasta) + tuple val(meta4), path(fasta_fai) output: tuple val(meta), path("*.bam"), path("*.bai"), emit: bam_and_bai diff --git a/modules/nf-core/sentieon/bwamem/meta.yml b/modules/nf-core/sentieon/bwamem/meta.yml index 3d1546c5..9987e8c9 100644 --- a/modules/nf-core/sentieon/bwamem/meta.yml +++ b/modules/nf-core/sentieon/bwamem/meta.yml @@ -33,10 +33,20 @@ input: type: file description: BWA genome index files pattern: "*.{amb,ann,bwt,pac,sa}" + - meta3: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] - fasta: type: file description: Genome fasta file pattern: "*.{fa,fasta}" + - meta4: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] - fasta_fai: type: file description: The index of the FASTA reference. From c0136b8acad08d7016b4dd3d6f0d849821b74120 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 16 Aug 2023 18:21:49 +0200 Subject: [PATCH 060/387] add filtervep2 --- modules/nf-core/ensemblvep/filtervep/main.nf | 50 +++++++++++++++++++ modules/nf-core/ensemblvep/filtervep/meta.yml | 47 +++++++++++++++++ 2 files changed, 97 insertions(+) create mode 100644 modules/nf-core/ensemblvep/filtervep/main.nf create mode 100644 modules/nf-core/ensemblvep/filtervep/meta.yml diff --git a/modules/nf-core/ensemblvep/filtervep/main.nf b/modules/nf-core/ensemblvep/filtervep/main.nf new file mode 100644 index 00000000..7fa2477a --- /dev/null +++ b/modules/nf-core/ensemblvep/filtervep/main.nf @@ -0,0 +1,50 @@ +process ENSEMBLVEP_FILTERVEP { + tag "$meta.id" + label 'process_single' + + conda "bioconda::ensembl-vep=110.0" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ensembl-vep:110.0--pl5321h2a3209d_0' : + 'biocontainers/ensembl-vep:110.0--pl5321h2a3209d_0' }" + + input: + tuple val(meta), path(input) + path (feature_file) + + output: + tuple val(meta), path("*.${extension}"), emit: output + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + extension = task.ext.suffix ?: "vcf" + """ + filter_vep \\ + $args \\ + --input_file $input \\ + --output_file ${prefix}.${extension} \\ + --only_matched + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ensemblvep: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + extension = task.ext.suffix ?: "vcf" + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ensemblvep: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//') + END_VERSIONS + """ +} + diff --git a/modules/nf-core/ensemblvep/filtervep/meta.yml b/modules/nf-core/ensemblvep/filtervep/meta.yml new file mode 100644 index 00000000..603c0040 --- /dev/null +++ b/modules/nf-core/ensemblvep/filtervep/meta.yml @@ -0,0 +1,47 @@ +name: "ensemblvep_filtervep" +description: Filter variants based on Ensembl Variant Effect Predictor (VEP) annotations. +keywords: + - annotation + - vcf + - tab + - filter +tools: + - ensemblvep: + description: | + VEP determines the effect of your variants (SNPs, insertions, deletions, CNVs + or structural variants) on genes, transcripts, and protein sequence, as well as regulatory regions. + homepage: https://www.ensembl.org/info/docs/tools/vep/index.html + documentation: https://www.ensembl.org/info/docs/tools/vep/script/index.html + licence: ["Apache-2.0"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - input: + type: file + description: VCF/TAB file annotated with vep + pattern: "*.{vcf,tab,tsv,txt}" + - feature_file: + type: file + description: File containing features on separate lines. To be used with --filter option. + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - output: + type: file + description: VCF/TAB file + pattern: "*.{vcf,tab,txt,tsv}" + +authors: + - "@ramprasadn" From 96ee2146fa375decbeb0d32cc2e53f8594214e51 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 16 Aug 2023 23:01:06 +0200 Subject: [PATCH 061/387] move filtervep --- modules/local/filter_vep.nf | 49 ------------------------------------- workflows/raredisease.nf | 24 ++++++++---------- 2 files changed, 10 insertions(+), 63 deletions(-) delete mode 100644 modules/local/filter_vep.nf diff --git a/modules/local/filter_vep.nf b/modules/local/filter_vep.nf deleted file mode 100644 index 3fbe8dad..00000000 --- a/modules/local/filter_vep.nf +++ /dev/null @@ -1,49 +0,0 @@ -process FILTER_VEP { - tag "$meta.id" - label 'process_low' - - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error("Local VEP module does not support Conda. Please use Docker / Singularity / Podman instead.") - } - container "docker.io/ensemblorg/ensembl-vep:release_107.0" - - input: - tuple val(meta), path(vcf) - path (select_feature_file) - - output: - tuple val(meta), path("*.ann_filter.vcf"), emit: vcf - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - filter_vep \\ - --format vcf \\ - --input_file $vcf \\ - --output_file ${prefix}.ann_filter.vcf \\ - --only_matched \\ - --filter \"HGNC_ID in ${select_feature_file}\" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - ensemblvep: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//') - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.ann_filter.vcf - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - ensemblvep: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//') - END_VERSIONS - """ -} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 0704b202..71cd9b8e 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -86,14 +86,6 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// MODULE: local modules -// - -include { FILTER_VEP as FILTER_VEP_SNV } from '../modules/local/filter_vep' -include { FILTER_VEP as FILTER_VEP_SV } from '../modules/local/filter_vep' -include { TABIX_BGZIPTABIX as BGZIPTABIX_SNV } from '../modules/nf-core/tabix/bgziptabix' -include { TABIX_BGZIPTABIX as BGZIPTABIX_SV } from '../modules/nf-core/tabix/bgziptabix' // // MODULE: Installed directly from nf-core/modules @@ -105,6 +97,10 @@ include { FASTQC } from '../modules/nf-core/fastq include { GATK4_SELECTVARIANTS } from '../modules/nf-core/gatk4/selectvariants/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' include { SMNCOPYNUMBERCALLER } from '../modules/nf-core/smncopynumbercaller/main' +include { ENSEMBLVEP_FILTERVEP as FILTERVEP_SNV } from '../modules/nf-core/ensemblvep/filtervep' +include { ENSEMBLVEP_FILTERVEP as FILTERVEP_SV } from '../modules/nf-core/ensemblvep/filtervep' +include { TABIX_BGZIPTABIX as BGZIPTABIX_SNV } from '../modules/nf-core/tabix/bgziptabix' +include { TABIX_BGZIPTABIX as BGZIPTABIX_SV } from '../modules/nf-core/tabix/bgziptabix' // // SUBWORKFLOWS @@ -430,13 +426,13 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(RANK_VARIANTS_SV.out.versions) - FILTER_VEP_SV( + FILTERVEP_SV( RANK_VARIANTS_SV.out.vcf, ch_vep_filters ) - ch_versions = ch_versions.mix(FILTER_VEP_SV.out.versions) + ch_versions = ch_versions.mix(FILTERVEP_SV.out.versions) - BGZIPTABIX_SV(FILTER_VEP_SV.out.vcf) + BGZIPTABIX_SV(FILTERVEP_SV.out.output) ch_versions = ch_versions.mix(BGZIPTABIX_SV.out.versions) } @@ -533,13 +529,13 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(RANK_VARIANTS_SNV.out.versions) - FILTER_VEP_SNV( + FILTERVEP_SNV( RANK_VARIANTS_SNV.out.vcf, ch_vep_filters ) - ch_versions = ch_versions.mix(FILTER_VEP_SNV.out.versions) + ch_versions = ch_versions.mix(FILTERVEP_SNV.out.versions) - BGZIPTABIX_SNV(FILTER_VEP_SNV.out.vcf) + BGZIPTABIX_SNV(FILTERVEP_SNV.out.output) ch_versions = ch_versions.mix(BGZIPTABIX_SNV.out.versions) } From b33a2f7527df090bd7686b725bc70146967a67ce Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 16 Aug 2023 23:59:10 +0200 Subject: [PATCH 062/387] fix sentieon call --- subworkflows/local/alignment/align_sentieon.nf | 2 +- subworkflows/local/mitochondria/align_and_call_MT.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/alignment/align_sentieon.nf b/subworkflows/local/alignment/align_sentieon.nf index 86447e81..044b0fb6 100644 --- a/subworkflows/local/alignment/align_sentieon.nf +++ b/subworkflows/local/alignment/align_sentieon.nf @@ -17,7 +17,7 @@ workflow ALIGN_SENTIEON { main: ch_versions = Channel.empty() - SENTIEON_BWAMEM ( ch_reads_input, ch_bwa_index, ch_genome_fasta.map{ meta, fasta -> fasta }, ch_genome_fai.map{ meta, fai -> fai }) + SENTIEON_BWAMEM ( ch_reads_input, ch_bwa_index, ch_genome_fasta, ch_genome_fai ) SENTIEON_BWAMEM.out .bam_and_bai diff --git a/subworkflows/local/mitochondria/align_and_call_MT.nf b/subworkflows/local/mitochondria/align_and_call_MT.nf index fb00739a..d857b11a 100644 --- a/subworkflows/local/mitochondria/align_and_call_MT.nf +++ b/subworkflows/local/mitochondria/align_and_call_MT.nf @@ -32,7 +32,7 @@ workflow ALIGN_AND_CALL_MT { BWAMEM2_MEM_MT (ch_fastq, ch_bwamem2index, true) - SENTIEON_BWAMEM_MT ( ch_fastq, ch_bwaindex, ch_fasta.map{ meta, fasta -> fasta }, ch_fai.map{ meta, fai -> fai } ) + SENTIEON_BWAMEM_MT ( ch_fastq, ch_bwaindex, ch_fasta, ch_fai ) Channel.empty() .mix(BWAMEM2_MEM_MT.out.bam, SENTIEON_BWAMEM_MT.out.bam_and_bai.map{ meta, bam, bai -> [meta, bam] }) From 31e72fb3c4516e16959e7a7feccddb3544e7261c Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 17 Aug 2023 11:07:24 +0200 Subject: [PATCH 063/387] add sentieon config --- conf/test.config | 7 ----- conf/test_sentieon.config | 59 +++++++++++++++++++++++++++++++++++++++ nextflow.config | 1 + 3 files changed, 60 insertions(+), 7 deletions(-) create mode 100644 conf/test_sentieon.config diff --git a/conf/test.config b/conf/test.config index 9093bfb6..8065aa8a 100644 --- a/conf/test.config +++ b/conf/test.config @@ -50,10 +50,3 @@ params { vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" vep_cache_version = 107 } - -process { - withLabel: 'sentieon' { - ext.sentieon_auth_mech_base64 = secrets.SENTIEON_AUTH_MECH_BASE64 - ext.sentieon_auth_data_base64 = secrets.SENTIEON_AUTH_DATA_BASE64 - } -} diff --git a/conf/test_sentieon.config b/conf/test_sentieon.config new file mode 100644 index 00000000..9093bfb6 --- /dev/null +++ b/conf/test_sentieon.config @@ -0,0 +1,59 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/raredisease -profile test, --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = '6.GB' + max_time = '2.h' + + // reference params + igenomes_ignore = true + mito_name = 'MT' + + // analysis params + skip_cnv_calling = true + + // Input data + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/testdata/samplesheet_trio.csv' + + // Genome references + fasta = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reference.fasta" + genome = 'GRCh37' + gnomad_af = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/gnomad_reformated.tab.gz" + intervals_wgs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/target_wgs.interval_list" + intervals_y = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/targetY.interval_list" + known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" + ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" + reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" + score_config_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" + score_config_sv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_sv.ini" + svdb_query_dbs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/svdb_querydb_files.csv" + target_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/target.bed" + variant_catalog = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/variant_catalog.json" + vcfanno_lua = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_functions.lua" + vcfanno_resources = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_resources.txt" + vcfanno_toml = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_config.toml" + vep_cache = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_cache_and_plugins.tar.gz" + vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" + vep_cache_version = 107 +} + +process { + withLabel: 'sentieon' { + ext.sentieon_auth_mech_base64 = secrets.SENTIEON_AUTH_MECH_BASE64 + ext.sentieon_auth_data_base64 = secrets.SENTIEON_AUTH_DATA_BASE64 + } +} diff --git a/nextflow.config b/nextflow.config index 185940df..2ec6a4df 100644 --- a/nextflow.config +++ b/nextflow.config @@ -196,6 +196,7 @@ profiles { test { includeConfig 'conf/test.config' } test_full { includeConfig 'conf/test_full.config' } test_one_sample { includeConfig 'conf/test_one_sample.config' } + test_sentieon { includeConfig 'conf/test_sentieon.config' } } // Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile From 6e99425ff919574b69832fe8624cb64623545b7e Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 17 Aug 2023 11:16:57 +0200 Subject: [PATCH 064/387] add dnascope --- modules.json | 353 +++++++++++++----- modules/local/sentieon/dnascope.nf | 62 --- modules/nf-core/sentieon/dnascope/main.nf | 91 +++++ modules/nf-core/sentieon/dnascope/meta.yml | 103 +++++ .../variant_calling/call_snv_sentieon.nf | 6 +- workflows/raredisease.nf | 4 +- 6 files changed, 465 insertions(+), 154 deletions(-) delete mode 100644 modules/local/sentieon/dnascope.nf create mode 100644 modules/nf-core/sentieon/dnascope/main.nf create mode 100644 modules/nf-core/sentieon/dnascope/meta.yml diff --git a/modules.json b/modules.json index b4e2c848..961708a2 100644 --- a/modules.json +++ b/modules.json @@ -8,435 +8,614 @@ "bcftools/annotate": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + 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+ "git_sha": "127edadc279e19da093fdd513926c6cdee82c306", + "installed_by": [ + "modules" + ] }, "sentieon/readwriter": { "branch": "master", "git_sha": "b28e4dde755117e8dab5d6e85e292f145b8b53c3", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "smncopynumbercaller": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "stranger": { "branch": "master", "git_sha": "0260e5d22372eae434816d6970dedf3f5adc0053", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "svdb/merge": { "branch": "master", "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "svdb/query": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "tabix/bgzip": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "tabix/bgziptabix": { "branch": "master", "git_sha": "591b71642820933dcb3c954c934b397bd00d8e5e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "tabix/tabix": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "tiddit/cov": { "branch": "master", "git_sha": "1c90a501d102b800c27697f5ef39a6e217ab1915", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "tiddit/sv": { "branch": "master", "git_sha": "1c90a501d102b800c27697f5ef39a6e217ab1915", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "ucsc/wigtobigwig": { "branch": "master", "git_sha": "66290981ab6038ea86177ade40b9449bc790b0ce", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "untar": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "upd": { "branch": "master", "git_sha": "9b159849d74f0eef251168c81c16da08215bbad5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "vcfanno": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] } } } } } -} +} \ No newline at end of file diff --git a/modules/local/sentieon/dnascope.nf b/modules/local/sentieon/dnascope.nf deleted file mode 100644 index d03fe2d4..00000000 --- a/modules/local/sentieon/dnascope.nf +++ /dev/null @@ -1,62 +0,0 @@ -process SENTIEON_DNASCOPE { - tag "$meta.id" - label 'process_high' - label 'sentieon' - - input: - tuple val(meta), path(bam), path(bai) - tuple val(meta2), path(fasta) - tuple val(meta3), path(fai) - tuple val(meta4), path(known_dbsnp) - tuple val(meta5), path(known_dbsnp_tbi) - path call_interval - path ml_model - - output: - tuple val(meta), path("*.vcf.gz") , emit: vcf - tuple val(meta), path("*.vcf.gz.tbi") , emit: index - tuple val(meta), path("*.vcf.gz"), path("*.vcf.gz.tbi"), emit: vcf_index - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - def interval = call_interval ? "--interval ${call_interval}" : '' - def dbsnp = known_dbsnp ? "-d ${known_dbsnp}" : '' - def model = ml_model ? "--model ${ml_model}" : '' - def prefix = task.ext.prefix ?: "${meta.id}" - - """ - sentieon driver \\ - -t $task.cpus \\ - -r $fasta \\ - $args \\ - -i $bam \\ - --algo DNAscope \\ - $dbsnp \\ - $interval \\ - $args2 \\ - $model \\ - ${prefix}.vcf.gz - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.vcf.gz - touch ${prefix}.vcf.gz.tbi - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g" ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/sentieon/dnascope/main.nf b/modules/nf-core/sentieon/dnascope/main.nf new file mode 100644 index 00000000..acc0ca7b --- /dev/null +++ b/modules/nf-core/sentieon/dnascope/main.nf @@ -0,0 +1,91 @@ +process SENTIEON_DNASCOPE { + tag "$meta.id" + label 'process_high' + label 'sentieon' + + secret 'SENTIEON_LICENSE_BASE64' + + container 'nf-core/sentieon:202112.06' + + input: + tuple val(meta), path(bam), path(bai) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fai) + tuple val(meta4), path(dbsnp) + tuple val(meta5), path(dbsnp_tbi) + tuple val(meta6), path(call_interval) + tuple val(meta7), path(ml_model) + + output: + tuple val(meta), path("*.vcf.gz") , emit: vcf + tuple val(meta), path("*.vcf.gz.tbi") , emit: index + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + } + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def interval = call_interval ? "--interval ${call_interval}" : '' + def dbsnp_str = dbsnp ? "-d ${dbsnp}" : '' + def model = ml_model ? "--model ${ml_model}" : '' + def prefix = task.ext.prefix ?: "${meta.id}" + def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: '' + def sentieon_auth_data_base64 = task.ext.sentieon_auth_data_base64 ?: '' + + """ + if [ "\${#SENTIEON_LICENSE_BASE64}" -lt "1500" ]; then # If the string SENTIEON_LICENSE_BASE64 is short, then it is an encrypted url. + export SENTIEON_LICENSE=\$(echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d) + else # Localhost license file + # The license file is stored as a nextflow variable like, for instance, this: + # nextflow secrets set SENTIEON_LICENSE_BASE64 \$(cat | base64 -w 0) + export SENTIEON_LICENSE=\$(mktemp) + echo -e "\$SENTIEON_LICENSE_BASE64" | base64 -d > \$SENTIEON_LICENSE + fi + + if [ ${sentieon_auth_mech_base64} ] && [ ${sentieon_auth_data_base64} ]; then + # If sentieon_auth_mech_base64 and sentieon_auth_data_base64 are non-empty strings, then Sentieon is mostly likely being run with some test-license. + export SENTIEON_AUTH_MECH=\$(echo -n "${sentieon_auth_mech_base64}" | base64 -d) + export SENTIEON_AUTH_DATA=\$(echo -n "${sentieon_auth_data_base64}" | base64 -d) + echo "Decoded and exported Sentieon test-license system environment variables" + fi + + sentieon driver \\ + -t $task.cpus \\ + -r $fasta \\ + $args \\ + $interval \\ + -i $bam \\ + --algo DNAscope \\ + $dbsnp_str \\ + $args2 \\ + $model \\ + ${prefix}.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g") + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + } + """ + touch ${prefix}.vcf.gz + touch ${prefix}.vcf.gz.tbi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sentieon: \$(echo \$(sentieon driver --version 2>&1) | sed -e "s/sentieon-genomics-//g" ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/sentieon/dnascope/meta.yml b/modules/nf-core/sentieon/dnascope/meta.yml new file mode 100644 index 00000000..82154b38 --- /dev/null +++ b/modules/nf-core/sentieon/dnascope/meta.yml @@ -0,0 +1,103 @@ +name: sentieon_dnascope +description: DNAscope algorithm performs an improved version of Haplotype variant calling. +keywords: + - dnascope + - sentieon + - variant_calling +tools: + - sentieon: + description: | + Sentieon® provides complete solutions for secondary DNA/RNA analysis for a variety of sequencing platforms, including short and long reads. + Our software improves upon BWA, STAR, Minimap2, GATK, HaplotypeCaller, Mutect, and Mutect2 based pipelines and is deployable on any generic-CPU-based computing system. + homepage: https://www.sentieon.com/ + documentation: https://www.sentieon.com/ +input: + - meta: + type: map + description: | + Groovy Map containing sample information. + e.g. [ id:'test', single_end:false ] + - meta2: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test' ] + - meta3: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test' ] + - meta4: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test' ] + - meta5: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test' ] + - meta6: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test' ] + - meta7: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test' ] + - bam: + type: file + description: BAM file. + pattern: "*.bam" + - bai: + type: file + description: BAI file + pattern: "*.bai" + - fasta: + type: file + description: Genome fasta file + pattern: "*.{fa,fasta}" + - fai: + type: file + description: Index of the genome fasta file + pattern: "*.fai" + - dbsnp: + type: file + description: Single Nucleotide Polymorphism database (dbSNP) file + pattern: "*.vcf.gz" + - dbsnp_tbi: + type: file + description: Index of the Single Nucleotide Polymorphism database (dbSNP) file + pattern: "*.vcf.gz.tbi" + - call_interval: + type: file + description: bed or interval_list file containing interval in the reference that will be used in the analysis + pattern: "*.{bed,interval_list}" + - ml_model: + type: file + description: machine learning model file + pattern: "*.model" + +output: + - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF file + pattern: "*.{vcf.gz}" + - index: + type: file + description: Index of the VCF file + pattern: "*.vcf.gz.tbi" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + +authors: + - "@ramprasadn" diff --git a/subworkflows/local/variant_calling/call_snv_sentieon.nf b/subworkflows/local/variant_calling/call_snv_sentieon.nf index bbcdbc19..b121a919 100644 --- a/subworkflows/local/variant_calling/call_snv_sentieon.nf +++ b/subworkflows/local/variant_calling/call_snv_sentieon.nf @@ -2,7 +2,7 @@ // A subworkflow to call SNVs by sentieon dnascope with a machine learning model. // -include { SENTIEON_DNASCOPE } from '../../../modules/local/sentieon/dnascope' +include { SENTIEON_DNASCOPE } from '../../../modules/nf-core/sentieon/dnascope/main' include { SENTIEON_DNAMODELAPPLY } from '../../../modules/nf-core/sentieon/dnamodelapply/main' include { BCFTOOLS_MERGE } from '../../../modules/nf-core/bcftools/merge/main' include { BCFTOOLS_NORM as SPLIT_MULTIALLELICS_SEN } from '../../../modules/nf-core/bcftools/norm/main' @@ -20,7 +20,7 @@ workflow CALL_SNV_SENTIEON { ch_dbsnp // channel: [mandatory] [ val(meta), path(vcf) ] ch_dbsnp_index // channel: [mandatory] [ val(meta), path(tbi) ] ch_call_interval // channel: [mandatory] [ path(interval) ] - ch_ml_model // channel: [mandatory] [ path(model) ] + ch_ml_model // channel: [mandatory] [ val(meta), path(model) ] ch_case_info // channel: [mandatory] [ val(case_info) ] main: @@ -28,7 +28,7 @@ workflow CALL_SNV_SENTIEON { SENTIEON_DNASCOPE ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_dbsnp, ch_dbsnp_index, ch_call_interval, ch_ml_model ) - SENTIEON_DNAMODELAPPLY ( SENTIEON_DNASCOPE.out.vcf_index, ch_genome_fasta, ch_genome_fai, [ [:], ch_ml_model ] ) + SENTIEON_DNAMODELAPPLY ( SENTIEON_DNASCOPE.out.vcf_index, ch_genome_fasta, ch_genome_fai, ch_ml_model ) BCF_FILTER_ONE (SENTIEON_DNAMODELAPPLY.out.vcf ) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 0704b202..53321013 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -234,8 +234,8 @@ workflow RAREDISEASE { : Channel.empty() ch_intervals_y = params.intervals_y ? Channel.fromPath(params.intervals_y).collect() : Channel.empty() - ch_ml_model = params.variant_caller.equals("sentieon") ? Channel.fromPath(params.ml_model).collect() - : Channel.value([]) + ch_ml_model = params.variant_caller.equals("sentieon") ? Channel.fromPath(params.ml_model).map {it -> [[id:it[0].simpleName], it]}.collect() + : Channel.value([[:],[]]) ch_mt_intervals = ch_references.mt_intervals ch_mtshift_backchain = ch_references.mtshift_backchain ch_mtshift_bwaindex = ch_references.mtshift_bwa_index From 1e2f1880f5b833750729370f4ca3b774bf37c662 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 17 Aug 2023 11:18:19 +0200 Subject: [PATCH 065/387] prettier --- modules.json | 350 +++++++++++++-------------------------------------- 1 file changed, 88 insertions(+), 262 deletions(-) diff --git a/modules.json b/modules.json index 961708a2..88716fda 100644 --- a/modules.json +++ b/modules.json @@ -8,614 +8,440 @@ "bcftools/annotate": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bcftools/concat": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - 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"installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "svdb/query": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "tabix/bgzip": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "tabix/bgziptabix": { "branch": "master", "git_sha": "591b71642820933dcb3c954c934b397bd00d8e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "tabix/tabix": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "tiddit/cov": { "branch": "master", "git_sha": "1c90a501d102b800c27697f5ef39a6e217ab1915", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "tiddit/sv": { "branch": "master", "git_sha": "1c90a501d102b800c27697f5ef39a6e217ab1915", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "ucsc/wigtobigwig": { "branch": "master", "git_sha": "66290981ab6038ea86177ade40b9449bc790b0ce", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "untar": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "upd": { "branch": "master", "git_sha": "9b159849d74f0eef251168c81c16da08215bbad5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "vcfanno": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } } } } -} \ No newline at end of file +} From 249bf0dec531aef877bbec1b410a05988209e56a Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 17 Aug 2023 11:21:46 +0200 Subject: [PATCH 066/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 244eda8d..00d4752e 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -20,6 +20,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Removing Sentieon-based BQSR. Recent Illumina sequencers tend to provide well-calibrated BQs, so BQSR may not provide much benefit [#402](https://github.com/nf-core/raredisease/pull/402) - Installed the nf-core version of the sentieon/dnamodelapply module [#403](https://github.com/nf-core/raredisease/pull/403) - Installed the nf-core version of the sentieon/wgsmetricsalgo module [#404](https://github.com/nf-core/raredisease/pull/404) +- Installed the nf-core version of the sentieon/dnascope module [#406](https://github.com/nf-core/raredisease/pull/406) ### `Fixed` From 71a6c2b27421dfbaca4a2d2c187b03993695908d Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 17 Aug 2023 11:32:28 +0200 Subject: [PATCH 067/387] fix error --- subworkflows/local/variant_calling/call_snv_sentieon.nf | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/subworkflows/local/variant_calling/call_snv_sentieon.nf b/subworkflows/local/variant_calling/call_snv_sentieon.nf index b121a919..50de505e 100644 --- a/subworkflows/local/variant_calling/call_snv_sentieon.nf +++ b/subworkflows/local/variant_calling/call_snv_sentieon.nf @@ -28,7 +28,9 @@ workflow CALL_SNV_SENTIEON { SENTIEON_DNASCOPE ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_dbsnp, ch_dbsnp_index, ch_call_interval, ch_ml_model ) - SENTIEON_DNAMODELAPPLY ( SENTIEON_DNASCOPE.out.vcf_index, ch_genome_fasta, ch_genome_fai, ch_ml_model ) + ch_dnamodelapply_in = SENTIEON_DNASCOPE.out.vcf.join(SENTIEON_DNASCOPE.out.index, failOnMismatch:true, failOnDuplicate:true) + + SENTIEON_DNAMODELAPPLY ( ch_dnamodelapply_in, ch_genome_fasta, ch_genome_fai, ch_ml_model ) BCF_FILTER_ONE (SENTIEON_DNAMODELAPPLY.out.vcf ) From 3d0b9bb8a46e6d3854319a3a61a33eb2fb2e7038 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 17 Aug 2023 17:59:09 +0200 Subject: [PATCH 068/387] update suffix --- conf/modules/align_sentieon.config | 1 + subworkflows/local/variant_calling/call_snv_sentieon.nf | 8 ++++---- workflows/raredisease.nf | 4 ++-- 3 files changed, 7 insertions(+), 6 deletions(-) diff --git a/conf/modules/align_sentieon.config b/conf/modules/align_sentieon.config index b39bad3c..3e002cb6 100644 --- a/conf/modules/align_sentieon.config +++ b/conf/modules/align_sentieon.config @@ -37,6 +37,7 @@ process { withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_DEDUP' { ext.args = { $params.rmdup ? "--rmdup" : '' } ext.prefix = { "${meta.id}_dedup" } + ext.suffix = ".bam" publishDir = [ enabled: !params.save_mapped_as_cram, path: { "${params.outdir}/alignment" }, diff --git a/subworkflows/local/variant_calling/call_snv_sentieon.nf b/subworkflows/local/variant_calling/call_snv_sentieon.nf index 50de505e..f7a36daf 100644 --- a/subworkflows/local/variant_calling/call_snv_sentieon.nf +++ b/subworkflows/local/variant_calling/call_snv_sentieon.nf @@ -15,11 +15,11 @@ include { BCFTOOLS_FILTER as BCF_FILTER_TWO } from '../../../modules/nf-c workflow CALL_SNV_SENTIEON { take: ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] - ch_genome_fasta // channel: [mandatory] [ path(fasta) ] - ch_genome_fai // channel: [mandatory] [ path(fai) ] + ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] ch_dbsnp // channel: [mandatory] [ val(meta), path(vcf) ] ch_dbsnp_index // channel: [mandatory] [ val(meta), path(tbi) ] - ch_call_interval // channel: [mandatory] [ path(interval) ] + ch_call_interval // channel: [mandatory] [ val(meta), path(interval) ] ch_ml_model // channel: [mandatory] [ val(meta), path(model) ] ch_case_info // channel: [mandatory] [ val(case_info) ] @@ -28,7 +28,7 @@ workflow CALL_SNV_SENTIEON { SENTIEON_DNASCOPE ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_dbsnp, ch_dbsnp_index, ch_call_interval, ch_ml_model ) - ch_dnamodelapply_in = SENTIEON_DNASCOPE.out.vcf.join(SENTIEON_DNASCOPE.out.index, failOnMismatch:true, failOnDuplicate:true) + ch_dnamodelapply_in = SENTIEON_DNASCOPE.out.vcf.join(SENTIEON_DNASCOPE.out.index) SENTIEON_DNAMODELAPPLY ( ch_dnamodelapply_in, ch_genome_fasta, ch_genome_fai, ch_ml_model ) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 53321013..88a657d4 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -209,8 +209,8 @@ workflow RAREDISEASE { ch_cadd_header = Channel.fromPath("$projectDir/assets/cadd_to_vcf_header_-1.0-.txt", checkIfExists: true).collect() ch_cadd_resources = params.cadd_resources ? Channel.fromPath(params.cadd_resources).collect() : Channel.value([]) - ch_call_interval = params.call_interval ? Channel.fromPath(params.call_interval).collect() - : Channel.value([]) + ch_call_interval = params.call_interval ? Channel.fromPath(params.call_interval).map {it -> [[id:it[0].simpleName], it]}.collect() + : Channel.value([[:],[]]) ch_dbsnp_tbi = params.known_dbsnp_tbi ? Channel.fromPath(params.known_dbsnp_tbi).map {it -> [[id:it[0].simpleName], it]}.collect() : ch_references.known_dbsnp_tbi.ifEmpty([[],[]]) ch_gcnvcaller_model = params.gcnvcaller_model ? Channel.fromPath(params.gcnvcaller_model).splitCsv ( header:true ) From 060f93294afc8be8db85b9d694165a2a9c9112bb Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 18 Aug 2023 16:40:53 +0200 Subject: [PATCH 069/387] fix --- assets/schema_input.json | 2 ++ 1 file changed, 2 insertions(+) diff --git a/assets/schema_input.json b/assets/schema_input.json index 165a1830..555f2c0a 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -53,6 +53,7 @@ "paternal_id": { "errorMessage": "Paternal ID cannot contain spaces", "meta": ["paternal"], + "default": "0", "anyOf": [ { "type": "string", @@ -67,6 +68,7 @@ "maternal_id": { "errorMessage": "Maternal ID cannot contain spaces", "meta": ["maternal"], + "default": "0", "anyOf": [ { "type": "string", From eda6ad7c5181bd75ba8dc50beb96b734d9519baa Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sat, 19 Aug 2023 12:14:27 +0200 Subject: [PATCH 070/387] update config --- conf/modules/raredisease.config | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/conf/modules/raredisease.config b/conf/modules/raredisease.config index 69419687..c4bab3d1 100644 --- a/conf/modules/raredisease.config +++ b/conf/modules/raredisease.config @@ -103,8 +103,9 @@ process { // process { - withName: '.*FILTER_VEP_SNV' { + withName: '.*FILTERVEP_SNV' { ext.prefix = { "${meta.id}_clinical_snv" } + ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } } withName: '.*BGZIPTABIX_SNV' { @@ -118,8 +119,9 @@ process { } process { - withName: '.*FILTER_VEP_SV' { + withName: '.*FILTERVEP_SV' { ext.prefix = { "${meta.id}_clinical_sv" } + ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } } withName: '.*BGZIPTABIX_SV' { From 94c95d31bf902b00e26c7d53667f5f6df875e355 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sat, 19 Aug 2023 14:01:53 +0200 Subject: [PATCH 071/387] fix storage error --- conf/test.config | 6 ++++++ 1 file changed, 6 insertions(+) diff --git a/conf/test.config b/conf/test.config index 8065aa8a..9a13845b 100644 --- a/conf/test.config +++ b/conf/test.config @@ -50,3 +50,9 @@ params { vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" vep_cache_version = 107 } + +process { + withName: '.*FILTERVEP.*' { + container = "docker.io/ensemblorg/ensembl-vep:release_107.0" + } +} From 087863861a112c9fc46c33e6bec4a4ac82e529c3 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 20 Aug 2023 00:43:13 +0200 Subject: [PATCH 072/387] update configs --- conf/test_one_sample.config | 6 ++++++ conf/test_sentieon.config | 4 ++++ 2 files changed, 10 insertions(+) diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index 7ffe7ba7..4fe746cc 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -50,3 +50,9 @@ params { vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" vep_cache_version = 107 } + +process { + withName: '.*FILTERVEP.*' { + container = "docker.io/ensemblorg/ensembl-vep:release_107.0" + } +} diff --git a/conf/test_sentieon.config b/conf/test_sentieon.config index 9093bfb6..f729c35e 100644 --- a/conf/test_sentieon.config +++ b/conf/test_sentieon.config @@ -56,4 +56,8 @@ process { ext.sentieon_auth_mech_base64 = secrets.SENTIEON_AUTH_MECH_BASE64 ext.sentieon_auth_data_base64 = secrets.SENTIEON_AUTH_DATA_BASE64 } + + withName: '.*FILTERVEP.*' { + container = "docker.io/ensemblorg/ensembl-vep:release_107.0" + } } From a6a5ee347d9b880695d3a62bc21f992474fa4035 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 21 Aug 2023 10:19:41 +0200 Subject: [PATCH 073/387] update configs --- conf/test.config | 2 +- conf/test_one_sample.config | 2 +- conf/test_sentieon.config | 2 +- 3 files changed, 3 insertions(+), 3 deletions(-) diff --git a/conf/test.config b/conf/test.config index 9a13845b..145c44b2 100644 --- a/conf/test.config +++ b/conf/test.config @@ -53,6 +53,6 @@ params { process { withName: '.*FILTERVEP.*' { - container = "docker.io/ensemblorg/ensembl-vep:release_107.0" + container = "docker.io/ensemblorg/ensembl-vep:release_110.1" } } diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index 4fe746cc..c83c8bd4 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -53,6 +53,6 @@ params { process { withName: '.*FILTERVEP.*' { - container = "docker.io/ensemblorg/ensembl-vep:release_107.0" + container = "docker.io/ensemblorg/ensembl-vep:release_110.1" } } diff --git a/conf/test_sentieon.config b/conf/test_sentieon.config index f729c35e..d3c4957b 100644 --- a/conf/test_sentieon.config +++ b/conf/test_sentieon.config @@ -58,6 +58,6 @@ process { } withName: '.*FILTERVEP.*' { - container = "docker.io/ensemblorg/ensembl-vep:release_107.0" + container = "docker.io/ensemblorg/ensembl-vep:release_110.1" } } From 7e3fe1bddb5c7173395ccdd379b2543b5764c1ff Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 21 Aug 2023 10:21:06 +0200 Subject: [PATCH 074/387] review suggestion --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 2ec6a4df..04b1b670 100644 --- a/nextflow.config +++ b/nextflow.config @@ -196,7 +196,7 @@ profiles { test { includeConfig 'conf/test.config' } test_full { includeConfig 'conf/test_full.config' } test_one_sample { includeConfig 'conf/test_one_sample.config' } - test_sentieon { includeConfig 'conf/test_sentieon.config' } + test_sentieon { includeConfig 'conf/test_sentieon.config' } } // Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile From c5fe695854b4d8935dbc2dc743e98d4d603de450 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 21 Aug 2023 13:04:03 +0200 Subject: [PATCH 075/387] test to 107 --- conf/test.config | 2 +- conf/test_one_sample.config | 2 +- conf/test_sentieon.config | 2 +- 3 files changed, 3 insertions(+), 3 deletions(-) diff --git a/conf/test.config b/conf/test.config index 145c44b2..9a13845b 100644 --- a/conf/test.config +++ b/conf/test.config @@ -53,6 +53,6 @@ params { process { withName: '.*FILTERVEP.*' { - container = "docker.io/ensemblorg/ensembl-vep:release_110.1" + container = "docker.io/ensemblorg/ensembl-vep:release_107.0" } } diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index c83c8bd4..4fe746cc 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -53,6 +53,6 @@ params { process { withName: '.*FILTERVEP.*' { - container = "docker.io/ensemblorg/ensembl-vep:release_110.1" + container = "docker.io/ensemblorg/ensembl-vep:release_107.0" } } diff --git a/conf/test_sentieon.config b/conf/test_sentieon.config index d3c4957b..f729c35e 100644 --- a/conf/test_sentieon.config +++ b/conf/test_sentieon.config @@ -58,6 +58,6 @@ process { } withName: '.*FILTERVEP.*' { - container = "docker.io/ensemblorg/ensembl-vep:release_110.1" + container = "docker.io/ensemblorg/ensembl-vep:release_107.0" } } From 9119dd40eae463350da04b964ffd864b6d0da544 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 21 Aug 2023 14:07:13 +0200 Subject: [PATCH 076/387] dumpsoftwareversions --- modules.json | 2 +- modules/nf-core/custom/dumpsoftwareversions/main.nf | 6 +++--- 2 files changed, 4 insertions(+), 4 deletions(-) diff --git a/modules.json b/modules.json index 8341a899..2109f33a 100644 --- a/modules.json +++ b/modules.json @@ -82,7 +82,7 @@ }, "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "05c280924b6c768d484c7c443dad5e605c4ff4b4", "installed_by": ["modules"] }, "deepvariant": { diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index ebc87273..c9d014b1 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.14" + conda "bioconda::multiqc=1.15" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.15--pyhdfd78af_0' : + 'biocontainers/multiqc:1.15--pyhdfd78af_0' }" input: path versions From 1c89bd464dc501f2c6730a41dd2783a3c3d66bf4 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 21 Aug 2023 15:42:00 +0200 Subject: [PATCH 077/387] update changelog --- CHANGELOG.md | 8 ++++++++ 1 file changed, 8 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 00d4752e..8bdca814 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -30,6 +30,14 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Make target bed file optional for WGS mode (Issue [#375](https://github.com/nf-core/raredisease/issues/375)) [#395](https://github.com/nf-core/raredisease/pull/395) - Added constraints to block the pipeline from running CollectWgsMetrics on WES samples [#396](https://github.com/nf-core/raredisease/pull/396) +### `Updated` + +| Tool | Old version | New version | +| ------------ | ----------- | ----------- | +| `filter_vep` | 107 | 110 | +| `multiqc` | 1.14 | 1.15 | +| `untar` | (grep 3.4) | (grep 3.11) | + ## v1.1.1 - Abu (Patch) [2023-07-26] ### `Fixed` From a2dfa8129fc53993cb99aa1e5b4b834a5428d866 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 21 Aug 2023 15:46:11 +0200 Subject: [PATCH 078/387] Update CHANGELOG.md Co-authored-by: Anders Sune Pedersen <37172585+asp8200@users.noreply.github.com> --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 8bdca814..4c483148 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -29,6 +29,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Sort ranked SV vcf before indexing with tabix [#393](https://github.com/nf-core/raredisease/pull/393) - Make target bed file optional for WGS mode (Issue [#375](https://github.com/nf-core/raredisease/issues/375)) [#395](https://github.com/nf-core/raredisease/pull/395) - Added constraints to block the pipeline from running CollectWgsMetrics on WES samples [#396](https://github.com/nf-core/raredisease/pull/396) +- Updated modules from nf-core [#412](https://github.com/nf-core/raredisease/pull/412) ### `Updated` From f6c17a1246cb5769aaf80ab5062b958866c4c91d Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 23 Aug 2023 13:52:41 +0200 Subject: [PATCH 079/387] add skip_vep_filter --- conf/modules/raredisease.config | 22 ++++++++++++++++++++++ nextflow.config | 1 + nextflow_schema.json | 7 ++++++- workflows/raredisease.nf | 4 ++++ 4 files changed, 33 insertions(+), 1 deletion(-) diff --git a/conf/modules/raredisease.config b/conf/modules/raredisease.config index c4bab3d1..a1b98f2a 100644 --- a/conf/modules/raredisease.config +++ b/conf/modules/raredisease.config @@ -103,12 +103,23 @@ process { // process { + withName: '.*RANK_VARIANTS_SNV' { + publishDir = [ + enabled: params.skip_vep_filter, + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + withName: '.*FILTERVEP_SNV' { + ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_clinical_snv" } ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } } withName: '.*BGZIPTABIX_SNV' { + ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_clinical_snv" } publishDir = [ path: { "${params.outdir}/rank_and_filter" }, @@ -119,12 +130,23 @@ process { } process { + withName: '.*RANK_VARIANTS_SV' { + publishDir = [ + enabled: params.skip_vep_filter, + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + withName: '.*FILTERVEP_SV' { + ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_clinical_sv" } ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } } withName: '.*BGZIPTABIX_SV' { + ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_clinical_sv" } publishDir = [ path: { "${params.outdir}/rank_and_filter" }, diff --git a/nextflow.config b/nextflow.config index 04b1b670..bd904b04 100644 --- a/nextflow.config +++ b/nextflow.config @@ -27,6 +27,7 @@ params { skip_cnv_calling = false skip_snv_annotation = false skip_sv_annotation = false + skip_vep_filter = false skip_mt_analysis = false gens_switch = false cadd_resources = null diff --git a/nextflow_schema.json b/nextflow_schema.json index f5216d76..ea742d93 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -386,7 +386,7 @@ "skip_cnv_calling": { "type": "boolean", "description": "Specifies whether or not to skip CNV calling.", - "fa_icon": "fas fa-book" + "fa_icon": "fas fa-toggle-on" }, "skip_mt_analysis": { "type": "boolean", @@ -402,6 +402,11 @@ "type": "boolean", "description": "Specifies whether or not to skip annotate structural variant subworkflow.", "fa_icon": "fas fa-toggle-on" + }, + "skip_vep_filter": { + "type": "boolean", + "description": "Specifies whether or not to skip filtering based on vep results.", + "fa_icon": "fas fa-toggle-on" } } }, diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index a2966fe7..1d1d8ddd 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -58,6 +58,10 @@ if (!params.skip_cnv_calling) { mandatoryParams += ["ploidy_model", "gcnvcaller_model"] } +if (!params.skip_vep_filter) { + mandatoryParams += ["vep_filters"] +} + def missingParamsCount = 0 for (param in mandatoryParams.unique()) { if (params[param] == null) { From f4ff764880dfbc33cb6632a1145486d1ebbf294e Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 23 Aug 2023 15:47:23 +0200 Subject: [PATCH 080/387] update docs --- docs/output.md | 2 +- docs/usage.md | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/docs/output.md b/docs/output.md index 6d07a3bd..48a63012 100644 --- a/docs/output.md +++ b/docs/output.md @@ -437,7 +437,7 @@ We recommend using vcfanno to annotate SNVs with precomputed CADD scores (files #### GENMOD -[GENMOD](https://github.com/Clinical-Genomics/genmod) is a simple to use command line tool for annotating and analyzing genomic variations in the VCF file format. GENMOD can annotate genetic patterns of inheritance in vcf:s with single or multiple families of arbitrary size. VCF file annotated by GENMOD are further filtered using [filter_vep from VEP](https://www.ensembl.org/info/docs/tools/vep/script/vep_filter.html) to separate clinically relevant variants. +[GENMOD](https://github.com/Clinical-Genomics/genmod) is a simple to use command line tool for annotating and analyzing genomic variations in the VCF file format. GENMOD can annotate genetic patterns of inheritance in vcf:s with single or multiple families of arbitrary size. VCF file annotated by GENMOD are further filtered using [filter_vep from VEP](https://www.ensembl.org/info/docs/tools/vep/script/vep_filter.html) to separate clinically relevant variants. You can skip the filtering step by setting --skip_vep_filter to true.
Output files diff --git a/docs/usage.md b/docs/usage.md index 57ad8780..24da77cd 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -224,7 +224,7 @@ See example cache [here](https://raw.githubusercontent.com/nf-core/test-datasets no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/gnomad_reformated.tab.gz).
6Used by GENMOD for ranking the variants. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/rank_model_snv.ini).
7Used by GENMOD while modeling the variants. Contains a list of loci that show [reduced penetrance](https://medlineplus.gov/genetics/understanding/inheritance/penetranceexpressivity/) in people. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/reduced_penetrance.tsv).
-8 This file contains a list of candidate genes (with [HGNC](https://www.genenames.org/) IDs) that is used to split the variants into canditate variants and research variants. Research variants contain all the variants, while candidate variants are a subset of research variants and are associated with candidate genes. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/hgnc.txt).
+8 This file contains a list of candidate genes (with [HGNC](https://www.genenames.org/) IDs) that is used to split the variants into canditate variants and research variants. Research variants contain all the variants, while candidate variants are a subset of research variants and are associated with candidate genes. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/hgnc.txt). Not required if --skip_vep_filter is set to true.
9Path to a folder containing cadd annotations. Equivalent of the data/annotations/ folder described [here](https://github.com/kircherlab/CADD-scripts/#manual-installation), and it is used to calculate CADD scores for small indels.
> NB: We use CADD only to annotate small indels. To annotate SNVs with precomputed CADD scores, pass the file containing CADD scores as a resource to vcfanno instead. Files containing the precomputed CADD scores for SNVs can be downloaded from [here](https://cadd.gs.washington.edu/download) (description: "All possible SNVs of GRCh3<7/8>/hg3<7/8>") From 53c5b9682e9523b9e47904e36c27e72015026b11 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 23 Aug 2023 15:55:07 +0200 Subject: [PATCH 081/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 4c483148..b834f2fe 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,6 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Added` - Use `nf-validation` plugin for parameter and samplesheet validation [#386](https://github.com/nf-core/raredisease/pull/386) +- A new parameter to skip filtering based on vep results [#416](https://github.com/nf-core/raredisease/pull/416) ### `Changed` From 3abdb850836d56b80f4a2f4d71b998a7d438c7f2 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 23 Aug 2023 22:13:48 +0200 Subject: [PATCH 082/387] align --- subworkflows/local/align.nf | 93 ++++++++++++++----- subworkflows/local/alignment/align_MT.nf | 56 +++++++++++ subworkflows/local/convert_mt_bam_to_fastq.nf | 36 +++++++ workflows/raredisease.nf | 30 +++--- 4 files changed, 182 insertions(+), 33 deletions(-) create mode 100644 subworkflows/local/alignment/align_MT.nf create mode 100644 subworkflows/local/convert_mt_bam_to_fastq.nf diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index 3455c3b9..f08ae7df 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -2,49 +2,100 @@ // Map to reference // -include { ALIGN_BWAMEM2 } from './alignment/align_bwamem2' -include { ALIGN_SENTIEON } from './alignment/align_sentieon' -include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main' +include { ALIGN_BWAMEM2 } from './alignment/align_bwamem2' +include { ALIGN_SENTIEON } from './alignment/align_sentieon' +include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main' +include { ALIGN_MT } from './alignment/align_MT' +include { ALIGN_MT as ALIGN_MT_SHIFT } from './alignment/align_MT' +include { CONVERT_MT_BAM_TO_FASTQ } from './convert_mt_bam_to_fastq' workflow ALIGN { take: - ch_reads_input // channel: [mandatory] [ val(meta), [path(reads)] ] - ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] - ch_bwa_index // channel: [mandatory] [ val(meta), path(index) ] - ch_bwamem2_index // channel: [mandatory] [ val(meta), path(index) ] - val_platform // string: [mandatory] illumina or a different technology + ch_reads // channel: [mandatory] [ val(meta), [path(reads)] ] + ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_genome_bwaindex // channel: [mandatory] [ val(meta), path(index) ] + ch_genome_bwamem2index // channel: [mandatory] [ val(meta), path(index) ] + ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] + ch_mtshift_bwaindex // channel: [mandatory] [ val(meta), path(index) ] + ch_mtshift_bwamem2index // channel: [mandatory] [ val(meta), path(index) ] + ch_mtshift_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_mtshift_dictionary // channel: [mandatory] [ val(meta), path(dict) ] + ch_mtshift_fai // channel: [mandatory] [ val(meta), path(fai) ] + val_platform // string: [mandatory] illumina or a different technology main: ch_versions = Channel.empty() ALIGN_BWAMEM2 ( // Triggered when params.aligner is set as bwamem2 - ch_reads_input, - ch_bwamem2_index, + ch_reads, + ch_genome_bwamem2index, ch_genome_fasta, ch_genome_fai, val_platform ) ALIGN_SENTIEON ( // Triggered when params.aligner is set as sentieon - ch_reads_input, + ch_reads, ch_genome_fasta, ch_genome_fai, - ch_bwa_index, + ch_genome_bwaindex, val_platform ) - ch_marked_bam = Channel.empty().mix(ALIGN_BWAMEM2.out.marked_bam, ALIGN_SENTIEON.out.marked_bam) - ch_marked_bai = Channel.empty().mix(ALIGN_BWAMEM2.out.marked_bai, ALIGN_SENTIEON.out.marked_bai) - ch_bam_bai = ch_marked_bam.join(ch_marked_bai, failOnMismatch:true, failOnDuplicate:true) + ch_genome_marked_bam = Channel.empty().mix(ALIGN_BWAMEM2.out.marked_bam, ALIGN_SENTIEON.out.marked_bam) + ch_genome_marked_bai = Channel.empty().mix(ALIGN_BWAMEM2.out.marked_bai, ALIGN_SENTIEON.out.marked_bai) + ch_genome_bam_bai = ch_genome_marked_bam.join(ch_genome_marked_bai, failOnMismatch:true, failOnDuplicate:true) - SAMTOOLS_VIEW( ch_bam_bai, ch_genome_fasta, [] ) + // PREPARING READS FOR MT ALIGNMENT + CONVERT_MT_BAM_TO_FASTQ ( + ch_genome_bam_bai, + ch_genome_fasta, + ch_genome_fai, + ch_genome_dictionary + ) + + ALIGN_MT ( + CONVERT_MT_BAM_TO_FASTQ.out.fastq, + CONVERT_MT_BAM_TO_FASTQ.out.bam, + ch_genome_bwaindex, + ch_genome_bwamem2index, + ch_genome_fasta, + ch_genome_dictionary, + ch_genome_fai + ) + + ALIGN_MT_SHIFT ( + CONVERT_MT_BAM_TO_FASTQ.out.fastq, + CONVERT_MT_BAM_TO_FASTQ.out.bam, + ch_mtshift_bwaindex, + ch_mtshift_bwamem2index, + ch_mtshift_fasta, + ch_mtshift_dictionary, + ch_mtshift_fai + ) + + ch_mt_marked_bam = Channel.empty().mix(ALIGN_MT.out.marked_bam, ALIGN_MT.out.marked_bam) + ch_mt_marked_bai = Channel.empty().mix(ALIGN_MT.out.marked_bai, ALIGN_MT.out.marked_bai) + ch_mt_bam_bai = ch_mt_marked_bam.join(ch_mt_marked_bai, failOnMismatch:true, failOnDuplicate:true) + + ch_mtshift_marked_bam = Channel.empty().mix(ALIGN_MT_SHIFT.out.marked_bam, ALIGN_MT_SHIFT.out.marked_bam) + ch_mtshift_marked_bai = Channel.empty().mix(ALIGN_MT_SHIFT.out.marked_bai, ALIGN_MT_SHIFT.out.marked_bai) + ch_mtshift_bam_bai = ch_mtshift_marked_bam.join(ch_mtshift_marked_bai, failOnMismatch:true, failOnDuplicate:true) + + SAMTOOLS_VIEW( ch_genome_bam_bai, ch_genome_fasta, [] ) ch_versions = Channel.empty().mix(ALIGN_BWAMEM2.out.versions, ALIGN_SENTIEON.out.versions) emit: - marked_bam = ch_marked_bam // channel: [ val(meta), path(bam) ] - marked_bai = ch_marked_bai // channel: [ val(meta), path(bai) ] - bam_bai = ch_bam_bai // channel: [ val(meta), path(bam), path(bai) ] - versions = ch_versions // channel: [ path(versions.yml) ] + genome_marked_bam = ch_genome_marked_bam // channel: [ val(meta), path(bam) ] + genome_marked_bai = ch_genome_marked_bai // channel: [ val(meta), path(bai) ] + genome_bam_bai = ch_genome_bam_bai // channel: [ val(meta), path(bam), path(bai) ] + mt_marked_bam = ch_mt_marked_bam // channel: [ val(meta), path(bam) ] + mt_marked_bai = ch_mt_marked_bai // channel: [ val(meta), path(bai) ] + mt_bam_bai = ch_mt_bam_bai // channel: [ val(meta), path(bam), path(bai) ] + mtshift_marked_bam = ch_mtshift_marked_bam // channel: [ val(meta), path(bam) ] + mtshift_marked_bai = ch_mtshift_marked_bai // channel: [ val(meta), path(bai) ] + mtshift_bam_bai = ch_mtshift_bam_bai // channel: [ val(meta), path(bam), path(bai) ] + versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/subworkflows/local/alignment/align_MT.nf b/subworkflows/local/alignment/align_MT.nf new file mode 100644 index 00000000..5ae8ace7 --- /dev/null +++ b/subworkflows/local/alignment/align_MT.nf @@ -0,0 +1,56 @@ +// +// Align and call MT +// + +include { SENTIEON_BWAMEM as SENTIEON_BWAMEM_MT } from '../../../modules/nf-core/sentieon/bwamem/main' +include { BWAMEM2_MEM as BWAMEM2_MEM_MT } from '../../../modules/nf-core/bwamem2/mem/main' +include { GATK4_MERGEBAMALIGNMENT as GATK4_MERGEBAMALIGNMENT_MT } from '../../../modules/nf-core/gatk4/mergebamalignment/main' +include { PICARD_ADDORREPLACEREADGROUPS as PICARD_ADDORREPLACEREADGROUPS_MT } from '../../../modules/nf-core/picard/addorreplacereadgroups/main' +include { PICARD_MARKDUPLICATES as PICARD_MARKDUPLICATES_MT } from '../../../modules/nf-core/picard/markduplicates/main' +include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_MT } from '../../../modules/nf-core/samtools/index/main' +include { SAMTOOLS_SORT as SAMTOOLS_SORT_MT } from '../../../modules/nf-core/samtools/sort/main' + +workflow ALIGN_MT { + take: + ch_fastq // channel: [mandatory] [ val(meta), [ path(reads) ] ] + ch_ubam // channel: [mandatory] [ val(meta), path(bam) ] + ch_bwaindex // channel: [mandatory for sentieon] [ val(meta), path(index) ] + ch_bwamem2index // channel: [mandatory for bwamem2] [ val(meta), path(index) ] + ch_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_dict // channel: [mandatory] [ val(meta), path(dict) ] + ch_fai // channel: [mandatory] [ val(meta), path(fai) ] + + main: + ch_versions = Channel.empty() + + BWAMEM2_MEM_MT (ch_fastq, ch_bwamem2index, true) + + SENTIEON_BWAMEM_MT ( ch_fastq, ch_bwaindex, ch_fasta, ch_fai ) + + Channel.empty() + .mix(BWAMEM2_MEM_MT.out.bam, SENTIEON_BWAMEM_MT.out.bam_and_bai.map{ meta, bam, bai -> [meta, bam] }) + .join(ch_ubam, failOnMismatch:true, failOnDuplicate:true) + .set {ch_bam_ubam} + + GATK4_MERGEBAMALIGNMENT_MT (ch_bam_ubam, ch_fasta, ch_dict) + + PICARD_ADDORREPLACEREADGROUPS_MT (GATK4_MERGEBAMALIGNMENT_MT.out.bam) + + PICARD_MARKDUPLICATES_MT (PICARD_ADDORREPLACEREADGROUPS_MT.out.bam, ch_fasta, ch_fai) + + SAMTOOLS_SORT_MT (PICARD_MARKDUPLICATES_MT.out.bam) + + SAMTOOLS_INDEX_MT(SAMTOOLS_SORT_MT.out.bam) + + ch_versions = ch_versions.mix(BWAMEM2_MEM_MT.out.versions.first()) + ch_versions = ch_versions.mix(GATK4_MERGEBAMALIGNMENT_MT.out.versions.first()) + ch_versions = ch_versions.mix(PICARD_ADDORREPLACEREADGROUPS_MT.out.versions.first()) + ch_versions = ch_versions.mix(PICARD_MARKDUPLICATES_MT.out.versions.first()) + ch_versions = ch_versions.mix(SAMTOOLS_SORT_MT.out.versions.first()) + ch_versions = ch_versions.mix(SAMTOOLS_INDEX_MT.out.versions.first()) + + emit: + marked_bam = SAMTOOLS_SORT_MT.out.bam // channel: [ val(meta), path(bam) ] + marked_bai = SAMTOOLS_INDEX_MT.out.bai // channel: [ val(meta), path(bai) ] + versions = ch_versions // channel: [ path(versions.yml) ] +} diff --git a/subworkflows/local/convert_mt_bam_to_fastq.nf b/subworkflows/local/convert_mt_bam_to_fastq.nf new file mode 100644 index 00000000..ca19ea82 --- /dev/null +++ b/subworkflows/local/convert_mt_bam_to_fastq.nf @@ -0,0 +1,36 @@ +// +// Prepare bam files for MT allignment +// + +include { GATK4_PRINTREADS as GATK4_PRINTREADS_MT } from '../../modules/nf-core/gatk4/printreads/main' +include { GATK4_REVERTSAM as GATK4_REVERTSAM_MT } from '../../modules/nf-core/gatk4/revertsam/main' +include { GATK4_SAMTOFASTQ as GATK4_SAMTOFASTQ_MT } from '../../modules/nf-core/gatk4/samtofastq/main' + +workflow CONVERT_MT_BAM_TO_FASTQ { + take: + ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_genome_dict // channel: [mandatory] [ val(meta), path(dict) ] + + main: + ch_versions = Channel.empty() + + // Outputs bam containing only MT + GATK4_PRINTREADS_MT ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_genome_dict ) + + // Removes alignment information + GATK4_REVERTSAM_MT ( GATK4_PRINTREADS_MT.out.bam ) + + // Outputs fastq files + GATK4_SAMTOFASTQ_MT ( GATK4_REVERTSAM_MT.out.bam ) + + ch_versions = ch_versions.mix(GATK4_PRINTREADS_MT.out.versions.first()) + ch_versions = ch_versions.mix(GATK4_REVERTSAM_MT.out.versions.first()) + ch_versions = ch_versions.mix(GATK4_SAMTOFASTQ_MT.out.versions.first()) + + emit: + fastq = GATK4_SAMTOFASTQ_MT.out.fastq // channel: [ val(meta), [ path(fastq) ] ] + bam = GATK4_REVERTSAM_MT.out.bam // channel: [ val(meta), path(bam) ] + versions = ch_versions // channel: [ path(versions.yml) ] +} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index a2966fe7..ce35c6bb 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -296,6 +296,12 @@ workflow RAREDISEASE { ch_genome_fai, ch_genome_bwaindex, ch_genome_bwamem2index, + ch_genome_dictionary, + ch_mtshift_bwaindex, + ch_mtshift_bwamem2index, + ch_mtshift_fasta, + ch_mtshift_dictionary, + ch_mtshift_fai, params.platform ) .set { ch_mapped } @@ -303,9 +309,9 @@ workflow RAREDISEASE { // BAM QUALITY CHECK QC_BAM ( - ch_mapped.marked_bam, - ch_mapped.marked_bai, - ch_mapped.bam_bai, + ch_mapped.genome_marked_bam, + ch_mapped.genome_marked_bai, + ch_mapped.genome_bam_bai, ch_genome_fasta, ch_genome_fai, ch_bait_intervals, @@ -318,7 +324,7 @@ workflow RAREDISEASE { // EXPANSIONHUNTER AND STRANGER CALL_REPEAT_EXPANSIONS ( - ch_mapped.bam_bai, + ch_mapped.genome_bam_bai, ch_variant_catalog, ch_case_info, ch_genome_fasta, @@ -327,12 +333,12 @@ workflow RAREDISEASE { ch_versions = ch_versions.mix(CALL_REPEAT_EXPANSIONS.out.versions) // STEP 1.7: SMNCOPYNUMBERCALLER - ch_mapped.bam_bai + ch_mapped.genome_bam_bai .collect{it[1]} .toList() .set { ch_bam_list } - ch_mapped.bam_bai + ch_mapped.genome_bam_bai .collect{it[2]} .toList() .set { ch_bai_list } @@ -349,7 +355,7 @@ workflow RAREDISEASE { // STEP 2: VARIANT CALLING CALL_SNV ( - ch_mapped.bam_bai, + ch_mapped.genome_bam_bai, ch_genome_fasta, ch_genome_fai, ch_dbsnp, @@ -361,9 +367,9 @@ workflow RAREDISEASE { ch_versions = ch_versions.mix(CALL_SNV.out.versions) CALL_STRUCTURAL_VARIANTS ( - ch_mapped.marked_bam, - ch_mapped.marked_bai, - ch_mapped.bam_bai, + ch_mapped.genome_marked_bam, + ch_mapped.genome_marked_bai, + ch_mapped.genome_bam_bai, ch_genome_bwaindex, ch_genome_fasta, ch_genome_fai, @@ -387,7 +393,7 @@ workflow RAREDISEASE { // GENS if (params.gens_switch) { GENS ( - ch_mapped.bam_bai, + ch_mapped.genome_bam_bai, CALL_SNV.out.vcf, ch_genome_fasta, ch_genome_fai, @@ -439,7 +445,7 @@ workflow RAREDISEASE { if (!params.skip_mt_analysis) { ANALYSE_MT ( - ch_mapped.bam_bai, + ch_mapped.genome_bam_bai, ch_cadd_header, ch_cadd_resources, ch_genome_bwaindex, From 1bdbbf68f4bbe23af14ab56e574c7530f1dd59c3 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 23 Aug 2023 22:17:05 +0200 Subject: [PATCH 083/387] comment --- subworkflows/local/alignment/align_MT.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/alignment/align_MT.nf b/subworkflows/local/alignment/align_MT.nf index 5ae8ace7..17ecc362 100644 --- a/subworkflows/local/alignment/align_MT.nf +++ b/subworkflows/local/alignment/align_MT.nf @@ -1,5 +1,5 @@ // -// Align and call MT +// Align MT // include { SENTIEON_BWAMEM as SENTIEON_BWAMEM_MT } from '../../../modules/nf-core/sentieon/bwamem/main' From 698e067bdbb2e28d8ee72973a342fcb65a0dd5c8 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 24 Aug 2023 00:37:05 +0200 Subject: [PATCH 084/387] update versions --- subworkflows/local/align.nf | 13 ++++++++----- 1 file changed, 8 insertions(+), 5 deletions(-) diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index f08ae7df..a705f16e 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -75,17 +75,20 @@ workflow ALIGN { ch_mtshift_fai ) - ch_mt_marked_bam = Channel.empty().mix(ALIGN_MT.out.marked_bam, ALIGN_MT.out.marked_bam) - ch_mt_marked_bai = Channel.empty().mix(ALIGN_MT.out.marked_bai, ALIGN_MT.out.marked_bai) + ch_mt_marked_bam = ALIGN_MT.out.marked_bam + ch_mt_marked_bai = ALIGN_MT.out.marked_bai ch_mt_bam_bai = ch_mt_marked_bam.join(ch_mt_marked_bai, failOnMismatch:true, failOnDuplicate:true) - ch_mtshift_marked_bam = Channel.empty().mix(ALIGN_MT_SHIFT.out.marked_bam, ALIGN_MT_SHIFT.out.marked_bam) - ch_mtshift_marked_bai = Channel.empty().mix(ALIGN_MT_SHIFT.out.marked_bai, ALIGN_MT_SHIFT.out.marked_bai) + ch_mtshift_marked_bam = ALIGN_MT_SHIFT.out.marked_bam + ch_mtshift_marked_bai = ALIGN_MT_SHIFT.out.marked_bai ch_mtshift_bam_bai = ch_mtshift_marked_bam.join(ch_mtshift_marked_bai, failOnMismatch:true, failOnDuplicate:true) SAMTOOLS_VIEW( ch_genome_bam_bai, ch_genome_fasta, [] ) - ch_versions = Channel.empty().mix(ALIGN_BWAMEM2.out.versions, ALIGN_SENTIEON.out.versions) + ch_versions = Channel.empty().mix(ALIGN_BWAMEM2.out.versions, + ALIGN_SENTIEON.out.versions, + ALIGN_MT.out.versions, + ALIGN_MT_SHIFT.out.versions) emit: genome_marked_bam = ch_genome_marked_bam // channel: [ val(meta), path(bam) ] From 97796fa2cf2af16d314bf84f0b4f6c988970c9d8 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 24 Aug 2023 00:38:15 +0200 Subject: [PATCH 085/387] mt snv calling --- subworkflows/local/call_snv.nf | 84 ++++++++++++++----- .../local/variant_calling/call_snv_MT.nf | 50 +++++++++++ workflows/raredisease.nf | 13 ++- 3 files changed, 124 insertions(+), 23 deletions(-) create mode 100644 subworkflows/local/variant_calling/call_snv_MT.nf diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index 7b63825e..75be4080 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -2,21 +2,31 @@ // call Single-nucleotide Varinats // -include { CALL_SNV_DEEPVARIANT } from './variant_calling/call_snv_deepvariant' -include { CALL_SNV_SENTIEON } from './variant_calling/call_snv_sentieon' -include { GATK4_SELECTVARIANTS } from '../../modules/nf-core/gatk4/selectvariants/main' - +include { CALL_SNV_DEEPVARIANT } from './variant_calling/call_snv_deepvariant' +include { CALL_SNV_SENTIEON } from './variant_calling/call_snv_sentieon' +include { CALL_SNV_MT } from './variant_calling/call_snv_MT' +include { CALL_SNV_MT as CALL_SNV_MT_SHIFT } from './variant_calling/call_snv_MT' +include { PICARD_LIFTOVERVCF } from '../../modules/nf-core/picard/liftovervcf/main' workflow CALL_SNV { take: - ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] - ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] - ch_known_dbsnp // channel: [optional] [ val(meta), path(vcf) ] - ch_known_dbsnp_tbi // channel: [optional] [ val(meta), path(tbi) ] - ch_call_interval // channel: [mandatory] [ path(intervals) ] - ch_ml_model // channel: [mandatory] [ path(model) ] - ch_case_info // channel: [mandatory] [ val(case_info) ] + ch_genome_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_mt_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_mtshift_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] + ch_mt_intervals // channel: [optional] [ path(interval_list) ] + ch_mtshift_fasta // channel: [optional] [ val(meta), path(fasta) ] + ch_mtshift_fai // channel: [optional] [ val(meta), path(fai) ] + ch_mtshift_dictionary // channel: [optional] [ val(meta), path(dict) ] + ch_mtshift_intervals // channel: [optional] [ path(interval_list) ] + ch_mtshift_backchain // channel: [mandatory] [ val(meta), path(back_chain) ] + ch_dbsnp // channel: [optional] [ val(meta), path(vcf) ] + ch_dbsnp_tbi // channel: [optional] [ val(meta), path(tbi) ] + ch_call_interval // channel: [mandatory] [ path(intervals) ] + ch_ml_model // channel: [mandatory] [ path(model) ] + ch_case_info // channel: [mandatory] [ val(case_info) ] main: ch_versions = Channel.empty() @@ -24,31 +34,63 @@ workflow CALL_SNV { ch_tabix = Channel.empty() CALL_SNV_DEEPVARIANT ( // triggered only when params.variant_caller is set as deepvariant - ch_bam_bai, + ch_genome_bam_bai, ch_genome_fasta, ch_genome_fai, ch_case_info ) CALL_SNV_SENTIEON( // triggered only when params.variant_caller is set as sentieon - ch_bam_bai, + ch_genome_bam_bai, ch_genome_fasta, ch_genome_fai, - ch_known_dbsnp, - ch_known_dbsnp_tbi, + ch_dbsnp, + ch_dbsnp_tbi, ch_call_interval, ch_ml_model, ch_case_info ) - ch_vcf = Channel.empty().mix(CALL_SNV_DEEPVARIANT.out.vcf, CALL_SNV_SENTIEON.out.vcf) - ch_tabix = Channel.empty().mix(CALL_SNV_DEEPVARIANT.out.tabix, CALL_SNV_SENTIEON.out.tabix) + CALL_SNV_MT( + ch_mt_bam_bai, + ch_genome_fasta, + ch_genome_fai, + ch_genome_dictionary, + ch_mt_intervals + ) + + CALL_SNV_MT_SHIFT( + ch_mtshift_bam_bai, + ch_mtshift_fasta, + ch_mtshift_fai, + ch_mtshift_dictionary, + ch_mtshift_intervals + ) + + // LIFTOVER VCF FROM REFERENCE MT TO SHIFTED MT + PICARD_LIFTOVERVCF ( + CALL_SNV_MT_SHIFT.out.vcf, + ch_genome_dictionary, + ch_genome_fasta, + ch_mtshift_backchain, + ) + + ch_genome_vcf = Channel.empty().mix(CALL_SNV_DEEPVARIANT.out.vcf, CALL_SNV_SENTIEON.out.vcf) + ch_genome_tabix = Channel.empty().mix(CALL_SNV_DEEPVARIANT.out.tabix, CALL_SNV_SENTIEON.out.tabix) + + ch_mt_vcf = CALL_SNV_MT.out.vcf + ch_mtshift_vcf = PICARD_LIFTOVERVCF.out.vcf_lifted ch_versions = ch_versions.mix(CALL_SNV_DEEPVARIANT.out.versions) ch_versions = ch_versions.mix(CALL_SNV_SENTIEON.out.versions) + ch_versions = ch_versions.mix(CALL_SNV_MT.out.versions) + ch_versions = ch_versions.mix(CALL_SNV_MT_SHIFT.out.versions) + ch_versions = ch_versions.mix(PICARD_LIFTOVERVCF.out.versions.first()) emit: - vcf = ch_vcf // channel: [ val(meta), path(vcf) ] - tabix = ch_tabix // channel: [ val(meta), path(tbi) ] - versions = ch_versions // channel: [ path(versions.yml) ] + genome_vcf = ch_genome_vcf // channel: [ val(meta), path(vcf) ] + genome_tabix = ch_genome_tabix // channel: [ val(meta), path(tbi) ] + mt_vcf = ch_mt_vcf // channel: [ val(meta), path(vcf) ] + mtshift_vcf = ch_mtshift_vcf // channel: [ val(meta), path(vcf) ] + versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/subworkflows/local/variant_calling/call_snv_MT.nf b/subworkflows/local/variant_calling/call_snv_MT.nf new file mode 100644 index 00000000..e91718ad --- /dev/null +++ b/subworkflows/local/variant_calling/call_snv_MT.nf @@ -0,0 +1,50 @@ +// +// Call SNV MT +// + +include { HAPLOCHECK as HAPLOCHECK_MT } from '../../../modules/nf-core/haplocheck/main' +include { GATK4_MUTECT2 as GATK4_MUTECT2_MT } from '../../../modules/nf-core/gatk4/mutect2/main' +include { GATK4_FILTERMUTECTCALLS as GATK4_FILTERMUTECTCALLS_MT } from '../../../modules/nf-core/gatk4/filtermutectcalls/main' +include { MT_DELETION } from '../../../modules/local/mt_deletion_script' +include { EKLIPSE as EKLIPSE_MT } from '../../../modules/nf-core/eklipse/main' + +workflow CALL_SNV_MT { + take: + ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_dict // channel: [mandatory] [ val(meta), path(dict) ] + ch_intervals // channel: [mandatory] [ path(interval_list) ] + + main: + ch_versions = Channel.empty() + + ch_bam_bai_int = ch_bam_bai.combine(ch_intervals) + + GATK4_MUTECT2_MT (ch_bam_bai_int, ch_fasta, ch_fai, ch_dict, [], [], [],[]) + + HAPLOCHECK_MT (GATK4_MUTECT2_MT.out.vcf) + + // Filter Mutect2 calls + ch_mutect_vcf = GATK4_MUTECT2_MT.out.vcf.join(GATK4_MUTECT2_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) + ch_mutect_out = ch_mutect_vcf.join(GATK4_MUTECT2_MT.out.stats, failOnMismatch:true, failOnDuplicate:true) + ch_to_filt = ch_mutect_out.map { + meta, vcf, tbi, stats -> + return [meta, vcf, tbi, stats, [], [], [], []] + } + + GATK4_FILTERMUTECTCALLS_MT (ch_to_filt, ch_fasta, ch_fai, ch_dict) + + ch_versions = ch_versions.mix(GATK4_MUTECT2_MT.out.versions.first()) + ch_versions = ch_versions.mix(HAPLOCHECK_MT.out.versions.first()) + ch_versions = ch_versions.mix(GATK4_FILTERMUTECTCALLS_MT.out.versions.first()) + + emit: + vcf = GATK4_FILTERMUTECTCALLS_MT.out.vcf // channel: [ val(meta), path(vcf) ] + tbi = GATK4_FILTERMUTECTCALLS_MT.out.tbi // channel: [ val(meta), path(tbi) ] + stats = GATK4_MUTECT2_MT.out.stats // channel: [ val(meta), path(stats) ] + filt_stats = GATK4_FILTERMUTECTCALLS_MT.out.stats // channel: [ val(meta), path(tsv) ] + txt = HAPLOCHECK_MT.out.txt // channel: [ val(meta), path(txt) ] + html = HAPLOCHECK_MT.out.html // channel: [ val(meta), path(html) ] + versions = ch_versions // channel: [ path(versions.yml) ] +} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index ce35c6bb..5112553b 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -356,8 +356,17 @@ workflow RAREDISEASE { // STEP 2: VARIANT CALLING CALL_SNV ( ch_mapped.genome_bam_bai, + ch_mapped.mt_bam_bai, + ch_mapped.mtshift_bam_bai, ch_genome_fasta, ch_genome_fai, + ch_genome_dictionary, + ch_mt_intervals, + ch_mtshift_fasta, + ch_mtshift_fai, + ch_mtshift_dictionary, + ch_mtshift_intervals, + ch_mtshift_backchain, ch_dbsnp, ch_dbsnp_tbi, ch_call_interval, @@ -385,7 +394,7 @@ workflow RAREDISEASE { // ped correspondence, sex check, ancestry check PEDDY_CHECK ( - CALL_SNV.out.vcf.join(CALL_SNV.out.tabix, failOnMismatch:true, failOnDuplicate:true), + CALL_SNV.out.genome_vcf.join(CALL_SNV.out.genome_tabix, failOnMismatch:true, failOnDuplicate:true), ch_pedfile ) ch_versions = ch_versions.mix(PEDDY_CHECK.out.versions) @@ -477,7 +486,7 @@ workflow RAREDISEASE { if (!params.skip_snv_annotation) { - ch_vcf = CALL_SNV.out.vcf.join(CALL_SNV.out.tabix, failOnMismatch:true, failOnDuplicate:true) + ch_vcf = CALL_SNV.out.genome_vcf.join(CALL_SNV.out.genome_tabix, failOnMismatch:true, failOnDuplicate:true) if (!params.skip_mt_analysis) { ch_vcf From 0c61ea688d12f3f9650e150f432e755d79f9af4f Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 24 Aug 2023 10:38:54 +0200 Subject: [PATCH 086/387] Update nextflow_schema.json --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index ea742d93..af85e653 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -405,7 +405,7 @@ }, "skip_vep_filter": { "type": "boolean", - "description": "Specifies whether or not to skip filtering based on vep results.", + "description": "Specifies whether or not to filter results based on a list of candidate genes specified in 'vep_filters'.", "fa_icon": "fas fa-toggle-on" } } From a4320bb64381556ec9777a7290df409fb3defa2e Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 24 Aug 2023 15:37:10 +0200 Subject: [PATCH 087/387] SV config --- conf/modules/align_MT.config | 98 +++++++++++++++++++ conf/modules/call_snv_MT.config | 43 ++++++++ conf/modules/call_sv_MT.config | 51 ++++++++++ nextflow.config | 3 + .../local/call_structural_variants.nf | 31 +++--- .../local/variant_calling/call_sv_MT.nf | 29 ++++++ workflows/raredisease.nf | 3 + 7 files changed, 247 insertions(+), 11 deletions(-) create mode 100644 conf/modules/align_MT.config create mode 100644 conf/modules/call_snv_MT.config create mode 100644 conf/modules/call_sv_MT.config create mode 100644 subworkflows/local/variant_calling/call_sv_MT.nf diff --git a/conf/modules/align_MT.config b/conf/modules/align_MT.config new file mode 100644 index 00000000..a01bfc60 --- /dev/null +++ b/conf/modules/align_MT.config @@ -0,0 +1,98 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. + ext.when = Conditional clause +---------------------------------------------------------------------------------------- +*/ + +// +// Mitochondria alignment options +// + +process { + + withName: '.*ALIGN_MT:BWAMEM2_MEM_MT' { + ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "bwamem2" } + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + } + + withName: '.*ALIGN_MT:SENTIEON_BWAMEM_MT' { + ext.args = { "-M -K 10000000 -R ${meta.read_group}" } + ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "sentieon" } + ext.prefix = { "${meta.id}.sorted" } + } + + withName: '.*ALIGN_MT:GATK4_MERGEBAMALIGNMENT_MT' { + ext.args = '--CREATE_INDEX true --MAX_GAPS -1 --SORT_ORDER queryname --INCLUDE_SECONDARY_ALIGNMENTS false --PAIRED_RUN false --VALIDATION_STRINGENCY LENIENT' + ext.prefix = { "${meta.id}_merged" } + } + + withName: '.*ALIGN_MT:PICARD_ADDORREPLACEREADGROUPS_MT' { + ext.args = { [ + "--VALIDATION_STRINGENCY LENIENT", + "--RGLB lib", + "--RGPL ILLUMINA", + "--RGPU barcode", + "--RGSM ${meta.id}" + ].join(' ').trim() } + } + + withName: '.*ALIGN_MT:PICARD_MARKDUPLICATES_MT' { + ext.args = '--VALIDATION_STRINGENCY LENIENT --CREATE_INDEX true' + ext.prefix = { "${meta.id}_markduplicates" } + } + + withName: '.*ALIGN_MT:SAMTOOLS_SORT_MT' { + ext.prefix = { "${meta.id}_sorted" } + } + +} + +// +// Shifted mitochondria alignment options +// + +process { + + withName: '.*ALIGN_MT_SHIFT:BWAMEM2_MEM_MT' { + ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "bwamem2" } + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + } + + withName: '.*ALIGN_MT_SHIFT:SENTIEON_BWAMEM_MT' { + ext.args = { "-M -K 10000000 -R ${meta.read_group}" } + ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "sentieon" } + ext.prefix = { "${meta.id}.sorted" } + } + + withName: '.*ALIGN_MT_SHIFT:GATK4_MERGEBAMALIGNMENT_MT' { + ext.args = '--CREATE_INDEX true --MAX_GAPS -1 --SORT_ORDER queryname --INCLUDE_SECONDARY_ALIGNMENTS false --PAIRED_RUN false --VALIDATION_STRINGENCY LENIENT' + ext.prefix = { "${meta.id}_merged_shifted" } + } + + withName: '.*ALIGN_MT_SHIFT:PICARD_ADDORREPLACEREADGROUPS_MT' { + ext.args = { [ + "--VALIDATION_STRINGENCY LENIENT", + "--RGLB lib", + "--RGPL ${params.platform}", + "--RGPU barcode", + "--RGSM ${meta.id}" + ].join(' ').trim() } + } + + withName: '.*ALIGN_MT_SHIFT:PICARD_MARKDUPLICATES_MT' { + ext.args = '--VALIDATION_STRINGENCY LENIENT --CREATE_INDEX true' + ext.prefix = { "${meta.id}_markduplicates_shifted" } + } + + withName: '.*ALIGN_MT_SHIFT:SAMTOOLS_SORT_MT' { + ext.prefix = { "${meta.id}_sorted_shifted" } + } + +} diff --git a/conf/modules/call_snv_MT.config b/conf/modules/call_snv_MT.config new file mode 100644 index 00000000..420929e5 --- /dev/null +++ b/conf/modules/call_snv_MT.config @@ -0,0 +1,43 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. + ext.when = Conditional clause +---------------------------------------------------------------------------------------- +*/ + +// +// Call SNVs in mitochondria +// + +process { + + withName: '.*CALL_SNV_MT:GATK4_MUTECT2_MT' { + ext.args = '--mitochondria-mode TRUE' + } + + withName: '.*CALL_SNV_MT:GATK4_FILTERMUTECTCALLS_MT' { + ext.prefix = { "${meta.id}_filtered" } + } +} + +// +// Call SNVs in shifted mitochondria +// + +process { + + withName: '.*CALL_SNV_MT_SHIFT:GATK4_MUTECT2_MT' { + ext.args = '--mitochondria-mode TRUE' + } + + withName: '.*CALL_SNV_MT_SHIFT:GATK4_FILTERMUTECTCALLS_MT' { + ext.prefix = { "${meta.id}_filtered_shifted" } + } + +} diff --git a/conf/modules/call_sv_MT.config b/conf/modules/call_sv_MT.config new file mode 100644 index 00000000..6b769e5c --- /dev/null +++ b/conf/modules/call_sv_MT.config @@ -0,0 +1,51 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. + ext.when = Conditional clause +---------------------------------------------------------------------------------------- +*/ + +// +// Call SV in mitochondria +// + +process { + + withName: '.*CALL_SV_MT:MT_DELETION' { + ext.args = '-s --insert-size 16000' + publishDir = [ + path: { "${params.outdir}/mt_sv" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*CALL_SV_MT:EKLIPSE' { + publishDir = [ + path: { "${params.outdir}/mt_sv" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } +} + +// +// Call SV in shifted mitochondria +// + +process { + + withName: '.*CALL_SV_MT_SHIFT:MT_DELETION' { + ext.when = false + } + + withName: '.*CALL_SV_MT_SHIFT:EKLIPSE_MT' { + ext.when = false + } +} diff --git a/nextflow.config b/nextflow.config index 04b1b670..1cd04d19 100644 --- a/nextflow.config +++ b/nextflow.config @@ -281,6 +281,9 @@ manifest { includeConfig 'conf/modules/raredisease.config' includeConfig 'conf/modules/align.config' includeConfig 'conf/modules/analyse_MT.config' +includeConfig 'conf/modules/align_MT.config' +includeConfig 'conf/modules/call_snv_MT.config' +includeConfig 'conf/modules/call_sv_MT.config' includeConfig 'conf/modules/call_snv.config' includeConfig 'conf/modules/call_structural_variants.config' includeConfig 'conf/modules/annotate_snvs.config' diff --git a/subworkflows/local/call_structural_variants.nf b/subworkflows/local/call_structural_variants.nf index 65016260..9e749add 100644 --- a/subworkflows/local/call_structural_variants.nf +++ b/subworkflows/local/call_structural_variants.nf @@ -2,21 +2,26 @@ // A nested subworkflow to call structural variants. // -include { CALL_SV_MANTA } from './variant_calling/call_sv_manta' -include { CALL_SV_TIDDIT } from './variant_calling/call_sv_tiddit' -include { SVDB_MERGE } from '../../modules/nf-core/svdb/merge/main' -include { CALL_SV_GERMLINECNVCALLER } from './variant_calling/call_sv_germlinecnvcaller' -include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix/main' +include { CALL_SV_MANTA } from './variant_calling/call_sv_manta' +include { CALL_SV_MT } from './variant_calling/call_sv_MT' +include { CALL_SV_MT as CALL_SV_MT_SHIFT } from './variant_calling/call_sv_MT' +include { CALL_SV_TIDDIT } from './variant_calling/call_sv_tiddit' +include { SVDB_MERGE } from '../../modules/nf-core/svdb/merge/main' +include { CALL_SV_GERMLINECNVCALLER } from './variant_calling/call_sv_germlinecnvcaller' +include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix/main' workflow CALL_STRUCTURAL_VARIANTS { take: - ch_bam // channel: [mandatory] [ val(meta), path(bam) ] - ch_bai // channel: [mandatory] [ val(meta), path(bai) ] - ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_genome_bam // channel: [mandatory] [ val(meta), path(bam) ] + ch_genome_bai // channel: [mandatory] [ val(meta), path(bai) ] + ch_genome_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_mt_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_mtshift_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] ch_bwa_index // channel: [mandatory] [ val(meta), path(index)] ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_mtshift_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_case_info // channel: [mandatory] [ val(case_info) ] ch_target_bed // channel: [mandatory for WES] [ val(meta), path(bed), path(tbi) ] ch_genome_dictionary // channel: [optional; used by mandatory for GATK's cnvcaller][ val(meta), path(dict) ] @@ -28,21 +33,25 @@ workflow CALL_STRUCTURAL_VARIANTS { main: ch_versions = Channel.empty() - CALL_SV_MANTA (ch_bam, ch_bai, ch_genome_fasta, ch_genome_fai, ch_case_info, ch_target_bed) + CALL_SV_MANTA (ch_genome_bam, ch_genome_bai, ch_genome_fasta, ch_genome_fai, ch_case_info, ch_target_bed) .diploid_sv_vcf .collect{it[1]} .set{ manta_vcf } - CALL_SV_TIDDIT (ch_bam_bai, ch_genome_fasta, ch_bwa_index, ch_case_info) + CALL_SV_TIDDIT (ch_genome_bam_bai, ch_genome_fasta, ch_bwa_index, ch_case_info) .vcf .collect{it[1]} .set { tiddit_vcf } - CALL_SV_GERMLINECNVCALLER (ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_readcount_intervals, ch_genome_dictionary, ch_ploidy_model, ch_gcnvcaller_model) + CALL_SV_GERMLINECNVCALLER (ch_genome_bam_bai, ch_genome_fasta, ch_genome_fai, ch_readcount_intervals, ch_genome_dictionary, ch_ploidy_model, ch_gcnvcaller_model) .genotyped_intervals_vcf .collect{it[1]} .set { gcnvcaller_vcf } + CALL_SV_MT (ch_mt_bam_bai, ch_genome_fasta) + + CALL_SV_MT_SHIFT (ch_mtshift_bam_bai, ch_mtshift_fasta) + //merge if (params.skip_cnv_calling) { tiddit_vcf diff --git a/subworkflows/local/variant_calling/call_sv_MT.nf b/subworkflows/local/variant_calling/call_sv_MT.nf new file mode 100644 index 00000000..c8fdff45 --- /dev/null +++ b/subworkflows/local/variant_calling/call_sv_MT.nf @@ -0,0 +1,29 @@ +// +// Call SV MT +// + +include { MT_DELETION } from '../../../modules/local/mt_deletion_script' +include { EKLIPSE } from '../../../modules/nf-core/eklipse/main' + +workflow CALL_SV_MT { + take: + ch_bam_bai // channel: [mandatory] [ val(meta), path(bam) ] + ch_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + + main: + ch_versions = Channel.empty() + + EKLIPSE(ch_bam_bai,[]) + + MT_DELETION(ch_bam_bai, ch_fasta) + + ch_versions = ch_versions.mix(EKLIPSE.out.versions.first()) + ch_versions = ch_versions.mix(MT_DELETION.out.versions.first()) + + emit: + eklipse_del = EKLIPSE.out.deletions // channel: [ val(meta), path(csv) ] + eklipse_genes = EKLIPSE.out.genes // channel: [ val(meta), path(csv) ] + eklipse_circos = EKLIPSE.out.circos // channel: [ val(meta), path(png) ] + mt_del_result = MT_DELETION.out.mt_del_result // channel: [ val(meta), path(txt) ] + versions = ch_versions // channel: [ path(versions.yml) ] +} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 5112553b..e88686e5 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -379,9 +379,12 @@ workflow RAREDISEASE { ch_mapped.genome_marked_bam, ch_mapped.genome_marked_bai, ch_mapped.genome_bam_bai, + ch_mapped.mt_bam_bai, + ch_mapped.mtshift_bam_bai, ch_genome_bwaindex, ch_genome_fasta, ch_genome_fai, + ch_mtshift_fasta, ch_case_info, ch_target_bed, ch_genome_dictionary, From 58d70e067373f37e9ae9c17233da6a9225fcb446 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 24 Aug 2023 17:23:12 +0200 Subject: [PATCH 088/387] postprocess --- ...nvs.config => annotate_genome_snvs.config} | 28 ++--- conf/modules/postptocess_MT_calls.config | 43 +++++++ nextflow.config | 4 +- ...notate_snvs.nf => annotate_genome_snvs.nf} | 5 +- subworkflows/local/annotate_mt_snvs.nf | 112 ++++++++++++++++++ subworkflows/local/call_snv.nf | 20 ++-- .../variant_calling/postprocess_MT_calls.nf | 112 ++++++++++++++++++ 7 files changed, 295 insertions(+), 29 deletions(-) rename conf/modules/{annotate_snvs.config => annotate_genome_snvs.config} (85%) create mode 100644 conf/modules/postptocess_MT_calls.config rename subworkflows/local/{annotate_snvs.nf => annotate_genome_snvs.nf} (98%) create mode 100644 subworkflows/local/annotate_mt_snvs.nf create mode 100644 subworkflows/local/variant_calling/postprocess_MT_calls.nf diff --git a/conf/modules/annotate_snvs.config b/conf/modules/annotate_genome_snvs.config similarity index 85% rename from conf/modules/annotate_snvs.config rename to conf/modules/annotate_genome_snvs.config index 093e94a1..0cbf5496 100644 --- a/conf/modules/annotate_snvs.config +++ b/conf/modules/annotate_genome_snvs.config @@ -16,43 +16,43 @@ // process { - withName: '.*ANNOTATE_SNVS:.*' { + withName: '.*ANNOTATE_GENOME_SNVS:.*' { ext.when = !params.skip_snv_annotation } - withName: '.*ANNOTATE_SNVS:VCFANNO' { + withName: '.*ANNOTATE_GENOME_SNVS:VCFANNO' { ext.prefix = { "${meta.id}_vcfanno" } publishDir = [ enabled: false ] } - withName: '.*ANNOTATE_SNVS:BCFTOOLS_ROH' { + withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_ROH' { ext.args = { "--samples ${meta.probands.join(",")} --skip-indels " } ext.prefix = { "${meta.id}_roh" } } - withName: '.*ANNOTATE_SNVS:RHOCALL_ANNOTATE' { + withName: '.*ANNOTATE_GENOME_SNVS:RHOCALL_ANNOTATE' { ext.args = { "--v14 " } ext.prefix = { "${meta.id}_rohann" } } - withName: '.*ANNOTATE_SNVS:VCFANNO' { + withName: '.*ANNOTATE_GENOME_SNVS:VCFANNO' { ext.prefix = { "${meta.id}_rohann_vcfanno" } } - withName: '.*ANNOTATE_SNVS:UPD_SITES' { + withName: '.*ANNOTATE_GENOME_SNVS:UPD_SITES' { ext.prefix = { "${meta.id}_rohann_vcfanno_upd_sites" } ext.args = {"--af-tag GNOMADAF --proband ${meta.upd_child} --mother ${meta.mother} --father ${meta.father} sites"} } - withName: '.*ANNOTATE_SNVS:UPD_REGIONS' { + withName: '.*ANNOTATE_GENOME_SNVS:UPD_REGIONS' { ext.prefix = { "${meta.id}_rohann_vcfanno_upd_regions" } ext.args = {"--af-tag GNOMADAF --proband ${meta.upd_child} --mother ${meta.mother} --father ${meta.father} regions --min-size 5 --min-sites 1"} ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun } } - withName: '.*ANNOTATE_SNVS:CHROMOGRAPH_SITES' { + withName: '.*ANNOTATE_GENOME_SNVS:CHROMOGRAPH_SITES' { ext.prefix = { "${meta7.id}_rohann_vcfanno_upd_sites_chromograph" } ext.args = { "--euploid" } tag = {"${meta7.id}"} @@ -63,7 +63,7 @@ process { ] } - withName: '.*ANNOTATE_SNVS:CHROMOGRAPH_REGIONS' { + withName: '.*ANNOTATE_GENOME_SNVS:CHROMOGRAPH_REGIONS' { ext.prefix = { "${meta6.id}_rohann_vcfanno_upd_regions_chromograph" } ext.args = { '--euploid' } ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun } @@ -75,16 +75,16 @@ process { ] } - withName: '.*ANNOTATE_SNVS:BCFTOOLS_VIEW' { + withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_VIEW' { ext.prefix = { "${meta.id}_rohann_vcfanno_filter" } ext.args = { '--output-type z --exclude "INFO/GNOMADAF > 0.70 | INFO/GNOMADAF_popmax > 0.70" ' } } - withName: '.*ANNOTATE_SNVS:GATK4_SELECTVARIANTS' { + withName: '.*ANNOTATE_GENOME_SNVS:GATK4_SELECTVARIANTS' { ext.prefix = { "${meta.id}_${intervals.simpleName}" } } - withName: '.*ANNOTATE_SNVS:ENSEMBLVEP_SNV' { + withName: '.*ANNOTATE_GENOME_SNVS:ENSEMBLVEP_SNV' { ext.prefix = { "${vcf.simpleName}_rohann_vcfanno_filter_vep" } ext.args = [ '--dir_plugins vep_cache/Plugins', @@ -103,7 +103,7 @@ process { ].join(' ') } - withName: '.*ANNOTATE_SNVS:BCFTOOLS_CONCAT' { + withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_CONCAT' { ext.prefix = { "${meta.id}_rohann_vcfanno_filter_vep" } publishDir = [ path: { "${params.outdir}/annotate_snv" }, @@ -112,7 +112,7 @@ process { ] } - withName: '.*ANNOTATE_SNVS:TABIX_BCFTOOLS_CONCAT' { + withName: '.*ANNOTATE_GENOME_SNVS:TABIX_BCFTOOLS_CONCAT' { publishDir = [ path: { "${params.outdir}/annotate_snv" }, mode: params.publish_dir_mode, diff --git a/conf/modules/postptocess_MT_calls.config b/conf/modules/postptocess_MT_calls.config new file mode 100644 index 00000000..3119012e --- /dev/null +++ b/conf/modules/postptocess_MT_calls.config @@ -0,0 +1,43 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. + ext.when = Conditional clause +---------------------------------------------------------------------------------------- +*/ + +// +// Mitochondrial annotation options +// + +process { + + withName: '.*POSTPROCESS_MT_CALLS:GATK4_MERGEVCFS_LIFT_UNLIFT_MT' { + ext.prefix = { "${meta.id}_merged" } + } + + withName: '.*POSTPROCESS_MT_CALLS:GATK4_VARIANTFILTRATION_MT' { + ext.prefix = { "${meta.id}_filt" } + } + + withName: '.*POSTPROCESS_MT_CALLS:SPLIT_MULTIALLELICS_MT' { + ext.args = '--output-type z --multiallelics -both' + ext.prefix = { "${meta.id}_split" } + } + + withName: '.*POSTPROCESS_MT_CALLS:REMOVE_DUPLICATES_MT' { + ext.args = '--output-type z --rm-dup none' + ext.prefix = { "${meta.id}_split_rmdup" } + } + + withName: '.*POSTPROCESS_MT_CALLS:BCFTOOLS_MERGE_MT' { + ext.args = '--output-type z' + ext.prefix = { "${meta.id}_merge_mt" } + } + +} diff --git a/nextflow.config b/nextflow.config index 1cd04d19..7c494c77 100644 --- a/nextflow.config +++ b/nextflow.config @@ -284,9 +284,11 @@ includeConfig 'conf/modules/analyse_MT.config' includeConfig 'conf/modules/align_MT.config' includeConfig 'conf/modules/call_snv_MT.config' includeConfig 'conf/modules/call_sv_MT.config' +includeConfig 'conf/modules/annotate_mt_snvs.config' includeConfig 'conf/modules/call_snv.config' +includeConfig 'conf/modules/postprocess_MT_calls.config' includeConfig 'conf/modules/call_structural_variants.config' -includeConfig 'conf/modules/annotate_snvs.config' +includeConfig 'conf/modules/annotate_genome_snvs.config' includeConfig 'conf/modules/annotate_structural_variants.config' includeConfig 'conf/modules/align_and_call_MT.config' includeConfig 'conf/modules/align_bwamem2.config' diff --git a/subworkflows/local/annotate_snvs.nf b/subworkflows/local/annotate_genome_snvs.nf similarity index 98% rename from subworkflows/local/annotate_snvs.nf rename to subworkflows/local/annotate_genome_snvs.nf index bd5fdfb8..06267872 100644 --- a/subworkflows/local/annotate_snvs.nf +++ b/subworkflows/local/annotate_genome_snvs.nf @@ -1,5 +1,5 @@ // -// A subworkflow to annotate snvs +// A subworkflow to annotate snvs in the genome // include { VCFANNO } from '../../modules/nf-core/vcfanno/main' @@ -20,7 +20,7 @@ include { TABIX_TABIX as TABIX_BCFTOOLS_VIEW } from '../../modules/nf-core/ta include { GATK4_SELECTVARIANTS } from '../../modules/nf-core/gatk4/selectvariants/main' include { ANNOTATE_CADD } from './annotation/annotate_cadd' -workflow ANNOTATE_SNVS { +workflow ANNOTATE_GENOME_SNVS { take: ch_vcf // channel: [mandatory] [ val(meta), path(vcf), path(tbi) ] @@ -46,7 +46,6 @@ workflow ANNOTATE_SNVS { RHOCALL_ANNOTATE (ch_vcf, BCFTOOLS_ROH.out.roh, []) - ZIP_TABIX_ROHCALL (RHOCALL_ANNOTATE.out.vcf) ZIP_TABIX_ROHCALL.out.gz_tbi diff --git a/subworkflows/local/annotate_mt_snvs.nf b/subworkflows/local/annotate_mt_snvs.nf new file mode 100644 index 00000000..400a58c1 --- /dev/null +++ b/subworkflows/local/annotate_mt_snvs.nf @@ -0,0 +1,112 @@ +// +// Merge and annotate MT +// + +include { GATK4_MERGEVCFS as GATK4_MERGEVCFS_LIFT_UNLIFT_MT } from '../../../modules/nf-core/gatk4/mergevcfs/main' +include { GATK4_VARIANTFILTRATION as GATK4_VARIANTFILTRATION_MT } from '../../../modules/nf-core/gatk4/variantfiltration/main' +include { BCFTOOLS_NORM as SPLIT_MULTIALLELICS_MT } from '../../../modules/nf-core/bcftools/norm/main' +include { TABIX_TABIX as TABIX_TABIX_MT } from '../../../modules/nf-core/tabix/tabix/main' +include { BCFTOOLS_NORM as REMOVE_DUPLICATES_MT } from '../../../modules/nf-core/bcftools/norm/main' +include { TABIX_TABIX as TABIX_TABIX_MT2 } from '../../../modules/nf-core/tabix/tabix/main' +include { BCFTOOLS_MERGE as BCFTOOLS_MERGE_MT } from '../../../modules/nf-core/bcftools/merge/main' +include { TABIX_TABIX as TABIX_TABIX_MERGE } from '../../../modules/nf-core/tabix/tabix/main' +include { TABIX_TABIX as TABIX_TABIX_MT3 } from '../../../modules/nf-core/tabix/tabix/main' +include { ENSEMBLVEP as ENSEMBLVEP_MT } from '../../../modules/local/ensemblvep/main' +include { HAPLOGREP2_CLASSIFY as HAPLOGREP2_CLASSIFY_MT } from '../../../modules/nf-core/haplogrep2/classify/main' +include { VCFANNO as VCFANNO_MT } from '../../../modules/nf-core/vcfanno/main' +include { ANNOTATE_CADD } from '../annotation/annotate_cadd' +include { TABIX_BGZIPTABIX as ZIP_TABIX_HMTNOTE } from '../../../modules/nf-core/tabix/bgziptabix/main' +include { HMTNOTE_ANNOTATE } from '../../../modules/nf-core/hmtnote/annotate/main' + +workflow ANNOTATE_MT_SNVS { + take: + ch_vcf1 // channel: [mandatory] [ val(meta), path(vcf) ] + ch_vcf2 // channel: [mandatory] [ val(meta), path(vcf) ] + ch_cadd_header // channel: [mandatory] [ path(txt) ] + ch_cadd_resources // channel: [mandatory] [ path(annotation) ] + ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_genome_dict // channel: [mandatory] [ val(meta), path(dict) ] + ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_vcfanno_resources // channel: [mandatory] [ path(resources) ] + ch_vcfanno_toml // channel: [mandatory] [ path(toml) ] + val_vep_genome // string: [mandatory] GRCh37 or GRCh38 + val_vep_cache_version // string: [mandatory] 107 + ch_vep_cache // channel: [mandatory] [ path(cache) ] + ch_case_info // channel: [mandatory] [ val(case_info) ] + + main: + ch_versions = Channel.empty() + + // Annotating with CADD + ANNOTATE_CADD ( + ch_annotation_in, + TABIX_TABIX_MERGE.out.tbi, + ch_cadd_header, + ch_cadd_resources + ) + + // Pick input for vep + ch_annotation_in + .combine(ANNOTATE_CADD.out.vcf.ifEmpty("null")) + .branch { it -> + merged: it[2].equals("null") + return [it[0], it[1]] + cadd: !(it[2].equals("null")) + return [it[2], it[3]] + } + .set { ch_for_mix } + ch_vep_in = ch_for_mix.merged.mix(ch_for_mix.cadd) + + // Annotating with ensembl Vep + ENSEMBLVEP_MT( + ch_vep_in, + ch_genome_fasta, + val_vep_genome, + "homo_sapiens", + val_vep_cache_version, + ch_vep_cache, + [] + ) + + // Running vcfanno + TABIX_TABIX_MT3(ENSEMBLVEP_MT.out.vcf_gz) + ENSEMBLVEP_MT.out.vcf_gz + .join(TABIX_TABIX_MT3.out.tbi, failOnMismatch:true, failOnDuplicate:true) + .map { meta, vcf, tbi -> return [meta, vcf, tbi, []]} + .set { ch_in_vcfanno } + + VCFANNO_MT(ch_in_vcfanno, ch_vcfanno_toml, [], ch_vcfanno_resources) + + // HMTNOTE ANNOTATE + HMTNOTE_ANNOTATE(VCFANNO_MT.out.vcf) + HMTNOTE_ANNOTATE.out.vcf.map{meta, vcf -> + return [meta, WorkflowRaredisease.replaceSpacesInInfoColumn(vcf, vcf.parent.toString(), vcf.baseName)] + } + .set { ch_hmtnote_reformatted } + ZIP_TABIX_HMTNOTE(ch_hmtnote_reformatted) + + // Prepare output + ch_vcf_out = ZIP_TABIX_HMTNOTE.out.gz_tbi.map{meta, vcf, tbi -> return [meta, vcf] } + ch_tbi_out = ZIP_TABIX_HMTNOTE.out.gz_tbi.map{meta, vcf, tbi -> return [meta, tbi] } + + // Running haplogrep2 + HAPLOGREP2_CLASSIFY_MT(ch_vep_in, "vcf.gz") + + ch_versions = ch_versions.mix(GATK4_MERGEVCFS_LIFT_UNLIFT_MT.out.versions.first()) + ch_versions = ch_versions.mix(GATK4_VARIANTFILTRATION_MT.out.versions.first()) + ch_versions = ch_versions.mix(SPLIT_MULTIALLELICS_MT.out.versions.first()) + ch_versions = ch_versions.mix(REMOVE_DUPLICATES_MT.out.versions.first()) + ch_versions = ch_versions.mix(BCFTOOLS_MERGE_MT.out.versions) + ch_versions = ch_versions.mix(ANNOTATE_CADD.out.versions) + ch_versions = ch_versions.mix(ENSEMBLVEP_MT.out.versions) + ch_versions = ch_versions.mix(VCFANNO_MT.out.versions) + ch_versions = ch_versions.mix(HMTNOTE_ANNOTATE.out.versions) + ch_versions = ch_versions.mix(HAPLOGREP2_CLASSIFY_MT.out.versions) + + emit: + haplog = HAPLOGREP2_CLASSIFY_MT.out.txt // channel: [ val(meta), path(txt) ] + vcf = ch_vcf_out // channel: [ val(meta), path(vcf) ] + tbi = ch_tbi_out // channel: [ val(meta), path(tbi) ] + report = ENSEMBLVEP_MT.out.report // channel: [ path(html) ] + versions = ch_versions // channel: [ path(versions.yml) ] +} diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index 75be4080..eb1d2ebf 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -6,7 +6,7 @@ include { CALL_SNV_DEEPVARIANT } from './variant_calling/call_snv_de include { CALL_SNV_SENTIEON } from './variant_calling/call_snv_sentieon' include { CALL_SNV_MT } from './variant_calling/call_snv_MT' include { CALL_SNV_MT as CALL_SNV_MT_SHIFT } from './variant_calling/call_snv_MT' -include { PICARD_LIFTOVERVCF } from '../../modules/nf-core/picard/liftovervcf/main' +include { POSTPROCESS_MT_CALLS } from './variant_calling/postprocess_MT_calls' workflow CALL_SNV { take: @@ -67,30 +67,28 @@ workflow CALL_SNV { ch_mtshift_intervals ) - // LIFTOVER VCF FROM REFERENCE MT TO SHIFTED MT - PICARD_LIFTOVERVCF ( - CALL_SNV_MT_SHIFT.out.vcf, - ch_genome_dictionary, + POSTPROCESS_MT_CALLS( + CALL_SNV_MT.out.vcf, + PICARD_LIFTOVERVCF.out.vcf_lifted, ch_genome_fasta, - ch_mtshift_backchain, + ch_genome_dictionary, + ch_genome_fai, + ch_case_info ) ch_genome_vcf = Channel.empty().mix(CALL_SNV_DEEPVARIANT.out.vcf, CALL_SNV_SENTIEON.out.vcf) ch_genome_tabix = Channel.empty().mix(CALL_SNV_DEEPVARIANT.out.tabix, CALL_SNV_SENTIEON.out.tabix) - - ch_mt_vcf = CALL_SNV_MT.out.vcf - ch_mtshift_vcf = PICARD_LIFTOVERVCF.out.vcf_lifted + ch_mt_vcf = POSTPROCESS_MT_CALLS.out.vcf ch_versions = ch_versions.mix(CALL_SNV_DEEPVARIANT.out.versions) ch_versions = ch_versions.mix(CALL_SNV_SENTIEON.out.versions) ch_versions = ch_versions.mix(CALL_SNV_MT.out.versions) ch_versions = ch_versions.mix(CALL_SNV_MT_SHIFT.out.versions) - ch_versions = ch_versions.mix(PICARD_LIFTOVERVCF.out.versions.first()) + ch_versions = ch_versions.mix(POSTPROCESS_MT_CALLS.out.versions) emit: genome_vcf = ch_genome_vcf // channel: [ val(meta), path(vcf) ] genome_tabix = ch_genome_tabix // channel: [ val(meta), path(tbi) ] mt_vcf = ch_mt_vcf // channel: [ val(meta), path(vcf) ] - mtshift_vcf = ch_mtshift_vcf // channel: [ val(meta), path(vcf) ] versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/subworkflows/local/variant_calling/postprocess_MT_calls.nf b/subworkflows/local/variant_calling/postprocess_MT_calls.nf new file mode 100644 index 00000000..6c9b7628 --- /dev/null +++ b/subworkflows/local/variant_calling/postprocess_MT_calls.nf @@ -0,0 +1,112 @@ +// +// Merge and annotate MT +// + +include { GATK4_MERGEVCFS as GATK4_MERGEVCFS_LIFT_UNLIFT_MT } from '../../../modules/nf-core/gatk4/mergevcfs/main' +include { GATK4_VARIANTFILTRATION as GATK4_VARIANTFILTRATION_MT } from '../../../modules/nf-core/gatk4/variantfiltration/main' +include { BCFTOOLS_NORM as SPLIT_MULTIALLELICS_MT } from '../../../modules/nf-core/bcftools/norm/main' +include { TABIX_TABIX as TABIX_TABIX_MT } from '../../../modules/nf-core/tabix/tabix/main' +include { BCFTOOLS_NORM as REMOVE_DUPLICATES_MT } from '../../../modules/nf-core/bcftools/norm/main' +include { TABIX_TABIX as TABIX_TABIX_MT2 } from '../../../modules/nf-core/tabix/tabix/main' +include { BCFTOOLS_MERGE as BCFTOOLS_MERGE_MT } from '../../../modules/nf-core/bcftools/merge/main' +include { TABIX_TABIX as TABIX_TABIX_MERGE } from '../../../modules/nf-core/tabix/tabix/main' +include { PICARD_LIFTOVERVCF } from '../../modules/nf-core/picard/liftovervcf/main' + +workflow POSTPROCESS_MT_CALLS { + take: + ch_mt_vcf // channel: [mandatory] [ val(meta), path(vcf) ] + ch_mtshift_vcf // channel: [mandatory] [ val(meta), path(vcf) ] + ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_genome_dict // channel: [mandatory] [ val(meta), path(dict) ] + ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_case_info // channel: [mandatory] [ val(case_info) ] + + main: + ch_versions = Channel.empty() + + // LIFTOVER VCF FROM REFERENCE MT TO SHIFTED MT + PICARD_LIFTOVERVCF ( + ch_mtshift_vcf, + ch_genome_dictionary, + ch_genome_fasta, + ch_mtshift_backchain, + ) + + ch_vcfs = ch_vcf1 + .join(ch_vcf2, remainder: true) + .map{ meta, vcf1, vcf2 -> + [meta, [vcf1, vcf2]] + } + GATK4_MERGEVCFS_LIFT_UNLIFT_MT( ch_vcfs, ch_genome_dict) + + // Filtering Variants + GATK4_MERGEVCFS_LIFT_UNLIFT_MT.out.vcf + .join(GATK4_MERGEVCFS_LIFT_UNLIFT_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) + .set { ch_filt_vcf } + GATK4_VARIANTFILTRATION_MT (ch_filt_vcf, ch_genome_fasta, ch_genome_fai, ch_genome_dict) + + // Spliting multiallelic calls + GATK4_VARIANTFILTRATION_MT.out.vcf + .join(GATK4_VARIANTFILTRATION_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) + .set { ch_in_split } + SPLIT_MULTIALLELICS_MT (ch_in_split, ch_genome_fasta) + TABIX_TABIX_MT(SPLIT_MULTIALLELICS_MT.out.vcf) + + // Removing duplicates and merging if there is more than one sample + SPLIT_MULTIALLELICS_MT.out.vcf + .join(TABIX_TABIX_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) + .set { ch_in_remdup } + REMOVE_DUPLICATES_MT(ch_in_remdup, ch_genome_fasta) + TABIX_TABIX_MT2(REMOVE_DUPLICATES_MT.out.vcf) + + REMOVE_DUPLICATES_MT.out.vcf + .collect{it[1]} + .ifEmpty([]) + .toList() + .set { file_list_vcf } + + TABIX_TABIX_MT2.out.tbi + .collect{it[1]} + .ifEmpty([]) + .toList() + .set { file_list_tbi } + + ch_case_info + .combine(file_list_vcf) + .combine(file_list_tbi) + .set { ch_rem_dup_vcf_tbi } + + ch_rem_dup_vcf_tbi.branch { + meta, vcf, tbi -> + single: vcf.size() == 1 + return [meta, vcf] + multiple: vcf.size() > 1 + return [meta, vcf, tbi] + }.set { ch_case_vcf } + + BCFTOOLS_MERGE_MT( ch_case_vcf.multiple, + ch_genome_fasta, + ch_genome_fai, + [] + ) + + BCFTOOLS_MERGE_MT.out.merged_variants + .mix(ch_case_vcf.single) + .set { ch_annotation_in } + + TABIX_TABIX_MERGE(ch_annotation_in) + + ch_versions = ch_versions.mix(PICARD_LIFTOVERVCF.out.versions.first()) + ch_versions = ch_versions.mix(GATK4_MERGEVCFS_LIFT_UNLIFT_MT.out.versions.first()) + ch_versions = ch_versions.mix(GATK4_VARIANTFILTRATION_MT.out.versions.first()) + ch_versions = ch_versions.mix(SPLIT_MULTIALLELICS_MT.out.versions.first()) + ch_versions = ch_versions.mix(REMOVE_DUPLICATES_MT.out.versions.first()) + ch_versions = ch_versions.mix(BCFTOOLS_MERGE_MT.out.versions) + + emit: + haplog = HAPLOGREP2_CLASSIFY_MT.out.txt // channel: [ val(meta), path(txt) ] + vcf = ch_vcf_out // channel: [ val(meta), path(vcf) ] + tbi = ch_tbi_out // channel: [ val(meta), path(tbi) ] + report = ENSEMBLVEP_MT.out.report // channel: [ path(html) ] + versions = ch_versions // channel: [ path(versions.yml) ] +} From 6bafacf60986d32d03b55dd16301d1cbee2053f2 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 24 Aug 2023 17:42:41 +0200 Subject: [PATCH 089/387] postprocess --- ...lls.config => postprocess_MT_calls.config} | 0 subworkflows/local/call_snv.nf | 3 ++- .../variant_calling/postprocess_MT_calls.nf | 19 +++++++++---------- workflows/raredisease.nf | 8 ++++---- 4 files changed, 15 insertions(+), 15 deletions(-) rename conf/modules/{postptocess_MT_calls.config => postprocess_MT_calls.config} (100%) diff --git a/conf/modules/postptocess_MT_calls.config b/conf/modules/postprocess_MT_calls.config similarity index 100% rename from conf/modules/postptocess_MT_calls.config rename to conf/modules/postprocess_MT_calls.config diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index eb1d2ebf..e11b598f 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -69,10 +69,11 @@ workflow CALL_SNV { POSTPROCESS_MT_CALLS( CALL_SNV_MT.out.vcf, - PICARD_LIFTOVERVCF.out.vcf_lifted, + CALL_SNV_MT_SHIFT.out.vcf, ch_genome_fasta, ch_genome_dictionary, ch_genome_fai, + ch_mtshift_backchain, ch_case_info ) diff --git a/subworkflows/local/variant_calling/postprocess_MT_calls.nf b/subworkflows/local/variant_calling/postprocess_MT_calls.nf index 6c9b7628..81738085 100644 --- a/subworkflows/local/variant_calling/postprocess_MT_calls.nf +++ b/subworkflows/local/variant_calling/postprocess_MT_calls.nf @@ -10,15 +10,16 @@ include { BCFTOOLS_NORM as REMOVE_DUPLICATES_MT } from '../../.. include { TABIX_TABIX as TABIX_TABIX_MT2 } from '../../../modules/nf-core/tabix/tabix/main' include { BCFTOOLS_MERGE as BCFTOOLS_MERGE_MT } from '../../../modules/nf-core/bcftools/merge/main' include { TABIX_TABIX as TABIX_TABIX_MERGE } from '../../../modules/nf-core/tabix/tabix/main' -include { PICARD_LIFTOVERVCF } from '../../modules/nf-core/picard/liftovervcf/main' +include { PICARD_LIFTOVERVCF } from '../../../modules/nf-core/picard/liftovervcf/main' workflow POSTPROCESS_MT_CALLS { take: ch_mt_vcf // channel: [mandatory] [ val(meta), path(vcf) ] ch_mtshift_vcf // channel: [mandatory] [ val(meta), path(vcf) ] ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_genome_dict // channel: [mandatory] [ val(meta), path(dict) ] + ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_mtshift_backchain // channel: [mandatory] [ val(meta), path(backchain) ] ch_case_info // channel: [mandatory] [ val(case_info) ] main: @@ -32,18 +33,18 @@ workflow POSTPROCESS_MT_CALLS { ch_mtshift_backchain, ) - ch_vcfs = ch_vcf1 - .join(ch_vcf2, remainder: true) + ch_vcfs = ch_mt_vcf + .join(PICARD_LIFTOVERVCF.out.vcf_lifted, remainder: true) .map{ meta, vcf1, vcf2 -> [meta, [vcf1, vcf2]] } - GATK4_MERGEVCFS_LIFT_UNLIFT_MT( ch_vcfs, ch_genome_dict) + GATK4_MERGEVCFS_LIFT_UNLIFT_MT( ch_vcfs, ch_genome_dictionary) // Filtering Variants GATK4_MERGEVCFS_LIFT_UNLIFT_MT.out.vcf .join(GATK4_MERGEVCFS_LIFT_UNLIFT_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) .set { ch_filt_vcf } - GATK4_VARIANTFILTRATION_MT (ch_filt_vcf, ch_genome_fasta, ch_genome_fai, ch_genome_dict) + GATK4_VARIANTFILTRATION_MT (ch_filt_vcf, ch_genome_fasta, ch_genome_fai, ch_genome_dictionary) // Spliting multiallelic calls GATK4_VARIANTFILTRATION_MT.out.vcf @@ -104,9 +105,7 @@ workflow POSTPROCESS_MT_CALLS { ch_versions = ch_versions.mix(BCFTOOLS_MERGE_MT.out.versions) emit: - haplog = HAPLOGREP2_CLASSIFY_MT.out.txt // channel: [ val(meta), path(txt) ] - vcf = ch_vcf_out // channel: [ val(meta), path(vcf) ] - tbi = ch_tbi_out // channel: [ val(meta), path(tbi) ] - report = ENSEMBLVEP_MT.out.report // channel: [ path(html) ] + vcf = ch_annotation_in // channel: [ val(meta), path(vcf) ] + tbi = TABIX_TABIX_MERGE.out.tbi // channel: [ val(meta), path(tbi) ] versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index e88686e5..70c1422a 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -110,7 +110,7 @@ include { ALIGN } from '../subworkflows/local/al include { ANALYSE_MT } from '../subworkflows/local/analyse_MT' include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SNV } from '../subworkflows/local/annotate_consequence_pli' include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SV } from '../subworkflows/local/annotate_consequence_pli' -include { ANNOTATE_SNVS } from '../subworkflows/local/annotate_snvs' +include { ANNOTATE_GENOME_SNVS } from '../subworkflows/local/annotate_genome_snvs' include { ANNOTATE_STRUCTURAL_VARIANTS } from '../subworkflows/local/annotate_structural_variants' include { CALL_REPEAT_EXPANSIONS } from '../subworkflows/local/call_repeat_expansions' include { CALL_SNV } from '../subworkflows/local/call_snv' @@ -502,7 +502,7 @@ workflow RAREDISEASE { ch_versions = ch_versions.mix(GATK4_SELECTVARIANTS.out.versions) } - ANNOTATE_SNVS ( + ANNOTATE_GENOME_SNVS ( ch_vcf, params.analysis_type, ch_cadd_header, @@ -519,11 +519,11 @@ workflow RAREDISEASE { ).set {ch_snv_annotate} ch_versions = ch_versions.mix(ch_snv_annotate.versions) - ch_snv_annotate = ANNOTATE_SNVS.out.vcf_ann + ch_snv_annotate = ANNOTATE_GENOME_SNVS.out.vcf_ann if (!params.skip_mt_analysis) { - ANNOTATE_SNVS.out.vcf_ann + ANNOTATE_GENOME_SNVS.out.vcf_ann .concat(ANALYSE_MT.out.vcf) .groupTuple() .set { ch_merged_vcf } From 7c5909d63428aafe85e93d1bbf5289fd386bcae1 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 24 Aug 2023 20:43:31 +0200 Subject: [PATCH 090/387] move annotate --- conf/modules/annotate_mt_snvs.config | 66 ++++++ conf/modules/call_sv_MT.config | 14 -- subworkflows/local/annotate_mt_snvs.nf | 50 ++-- subworkflows/local/call_snv.nf | 29 ++- .../local/call_structural_variants.nf | 2 +- workflows/raredisease.nf | 216 +++++++++--------- 6 files changed, 221 insertions(+), 156 deletions(-) create mode 100644 conf/modules/annotate_mt_snvs.config diff --git a/conf/modules/annotate_mt_snvs.config b/conf/modules/annotate_mt_snvs.config new file mode 100644 index 00000000..68e01af1 --- /dev/null +++ b/conf/modules/annotate_mt_snvs.config @@ -0,0 +1,66 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. + ext.when = Conditional clause +---------------------------------------------------------------------------------------- +*/ + +// +// Mitochondrial annotation options +// + +process { + + + withName: '.*ANNOTATE_MT_SNVS:ENSEMBLVEP_MT' { + ext.args = [ + '--dir_plugins vep_cache/Plugins', + '--plugin LoFtool,vep_cache/LoFtool_scores.txt', + '--plugin pLI,vep_cache/pLI_values_107.txt', + '--plugin SpliceAI,snv=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', + '--plugin MaxEntScan,vep_cache/fordownload,SWA,NCSS', + '--distance 0', + '--buffer_size 20000', + '--format vcf --fork 4 --max_sv_size 248956422', + '--appris --biotype --cache --canonical --ccds --compress_output bgzip', + '--domains --exclude_predicted --force_overwrite', + '--hgvs --humdiv --no_progress --no_stats --numbers', + '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl --vcf', + '--uniprot' + ].join(' ') + } + + withName: '.*ANNOTATE_MT_SNVS:ZIP_TABIX_HMTNOTE' { + ext.prefix = { "${meta.id}_vep_vcfanno_hmtnote_mt" } + publishDir = [ + path: { "${params.outdir}/annotate_mt" }, + mode: params.publish_dir_mode, + pattern: "*{vcf.gz,vcf.gz.tbi}", + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*ANNOTATE_MT_SNVS:HMTNOTE_ANNOTATE' { + ext.prefix = { "${meta.id}_vep_vcfanno_hmtnote_mt" } + ext.args = '--offline' + publishDir = [ + enabled: false + ] + } + + withName: '.*ANNOTATE_MT_SNVS:HAPLOGREP2_CLASSIFY_MT' { + ext.prefix = { "${meta.id}_haplogrep" } + publishDir = [ + path: { "${params.outdir}/annotate_mt" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + +} diff --git a/conf/modules/call_sv_MT.config b/conf/modules/call_sv_MT.config index 6b769e5c..288ca425 100644 --- a/conf/modules/call_sv_MT.config +++ b/conf/modules/call_sv_MT.config @@ -35,17 +35,3 @@ process { } } -// -// Call SV in shifted mitochondria -// - -process { - - withName: '.*CALL_SV_MT_SHIFT:MT_DELETION' { - ext.when = false - } - - withName: '.*CALL_SV_MT_SHIFT:EKLIPSE_MT' { - ext.when = false - } -} diff --git a/subworkflows/local/annotate_mt_snvs.nf b/subworkflows/local/annotate_mt_snvs.nf index 400a58c1..d6ce5659 100644 --- a/subworkflows/local/annotate_mt_snvs.nf +++ b/subworkflows/local/annotate_mt_snvs.nf @@ -2,51 +2,48 @@ // Merge and annotate MT // -include { GATK4_MERGEVCFS as GATK4_MERGEVCFS_LIFT_UNLIFT_MT } from '../../../modules/nf-core/gatk4/mergevcfs/main' -include { GATK4_VARIANTFILTRATION as GATK4_VARIANTFILTRATION_MT } from '../../../modules/nf-core/gatk4/variantfiltration/main' -include { BCFTOOLS_NORM as SPLIT_MULTIALLELICS_MT } from '../../../modules/nf-core/bcftools/norm/main' -include { TABIX_TABIX as TABIX_TABIX_MT } from '../../../modules/nf-core/tabix/tabix/main' -include { BCFTOOLS_NORM as REMOVE_DUPLICATES_MT } from '../../../modules/nf-core/bcftools/norm/main' -include { TABIX_TABIX as TABIX_TABIX_MT2 } from '../../../modules/nf-core/tabix/tabix/main' -include { BCFTOOLS_MERGE as BCFTOOLS_MERGE_MT } from '../../../modules/nf-core/bcftools/merge/main' -include { TABIX_TABIX as TABIX_TABIX_MERGE } from '../../../modules/nf-core/tabix/tabix/main' -include { TABIX_TABIX as TABIX_TABIX_MT3 } from '../../../modules/nf-core/tabix/tabix/main' -include { ENSEMBLVEP as ENSEMBLVEP_MT } from '../../../modules/local/ensemblvep/main' -include { HAPLOGREP2_CLASSIFY as HAPLOGREP2_CLASSIFY_MT } from '../../../modules/nf-core/haplogrep2/classify/main' -include { VCFANNO as VCFANNO_MT } from '../../../modules/nf-core/vcfanno/main' -include { ANNOTATE_CADD } from '../annotation/annotate_cadd' -include { TABIX_BGZIPTABIX as ZIP_TABIX_HMTNOTE } from '../../../modules/nf-core/tabix/bgziptabix/main' -include { HMTNOTE_ANNOTATE } from '../../../modules/nf-core/hmtnote/annotate/main' +include { GATK4_MERGEVCFS as GATK4_MERGEVCFS_LIFT_UNLIFT_MT } from '../../modules/nf-core/gatk4/mergevcfs/main' +include { GATK4_VARIANTFILTRATION as GATK4_VARIANTFILTRATION_MT } from '../../modules/nf-core/gatk4/variantfiltration/main' +include { BCFTOOLS_NORM as SPLIT_MULTIALLELICS_MT } from '../../modules/nf-core/bcftools/norm/main' +include { TABIX_TABIX as TABIX_TABIX_MT } from '../../modules/nf-core/tabix/tabix/main' +include { BCFTOOLS_NORM as REMOVE_DUPLICATES_MT } from '../../modules/nf-core/bcftools/norm/main' +include { TABIX_TABIX as TABIX_TABIX_MT2 } from '../../modules/nf-core/tabix/tabix/main' +include { BCFTOOLS_MERGE as BCFTOOLS_MERGE_MT } from '../../modules/nf-core/bcftools/merge/main' +include { TABIX_TABIX as TABIX_TABIX_MERGE } from '../../modules/nf-core/tabix/tabix/main' +include { TABIX_TABIX as TABIX_TABIX_MT3 } from '../../modules/nf-core/tabix/tabix/main' +include { ENSEMBLVEP as ENSEMBLVEP_MT } from '../../modules/local/ensemblvep/main' +include { HAPLOGREP2_CLASSIFY as HAPLOGREP2_CLASSIFY_MT } from '../../modules/nf-core/haplogrep2/classify/main' +include { VCFANNO as VCFANNO_MT } from '../../modules/nf-core/vcfanno/main' +include { ANNOTATE_CADD } from './annotation/annotate_cadd' +include { TABIX_BGZIPTABIX as ZIP_TABIX_HMTNOTE } from '../../modules/nf-core/tabix/bgziptabix/main' +include { HMTNOTE_ANNOTATE } from '../../modules/nf-core/hmtnote/annotate/main' workflow ANNOTATE_MT_SNVS { take: - ch_vcf1 // channel: [mandatory] [ val(meta), path(vcf) ] - ch_vcf2 // channel: [mandatory] [ val(meta), path(vcf) ] + ch_mt_vcf // channel: [mandatory] [ val(meta), path(vcf) ] + ch_mt_tbi // channel: [mandatory] [ val(meta), path(tbi) ] ch_cadd_header // channel: [mandatory] [ path(txt) ] ch_cadd_resources // channel: [mandatory] [ path(annotation) ] ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_genome_dict // channel: [mandatory] [ val(meta), path(dict) ] - ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] ch_vcfanno_resources // channel: [mandatory] [ path(resources) ] ch_vcfanno_toml // channel: [mandatory] [ path(toml) ] val_vep_genome // string: [mandatory] GRCh37 or GRCh38 val_vep_cache_version // string: [mandatory] 107 ch_vep_cache // channel: [mandatory] [ path(cache) ] - ch_case_info // channel: [mandatory] [ val(case_info) ] main: ch_versions = Channel.empty() // Annotating with CADD ANNOTATE_CADD ( - ch_annotation_in, - TABIX_TABIX_MERGE.out.tbi, + ch_mt_vcf, + ch_mt_tbi, ch_cadd_header, ch_cadd_resources ) // Pick input for vep - ch_annotation_in + ch_mt_vcf .combine(ANNOTATE_CADD.out.vcf.ifEmpty("null")) .branch { it -> merged: it[2].equals("null") @@ -92,11 +89,6 @@ workflow ANNOTATE_MT_SNVS { // Running haplogrep2 HAPLOGREP2_CLASSIFY_MT(ch_vep_in, "vcf.gz") - ch_versions = ch_versions.mix(GATK4_MERGEVCFS_LIFT_UNLIFT_MT.out.versions.first()) - ch_versions = ch_versions.mix(GATK4_VARIANTFILTRATION_MT.out.versions.first()) - ch_versions = ch_versions.mix(SPLIT_MULTIALLELICS_MT.out.versions.first()) - ch_versions = ch_versions.mix(REMOVE_DUPLICATES_MT.out.versions.first()) - ch_versions = ch_versions.mix(BCFTOOLS_MERGE_MT.out.versions) ch_versions = ch_versions.mix(ANNOTATE_CADD.out.versions) ch_versions = ch_versions.mix(ENSEMBLVEP_MT.out.versions) ch_versions = ch_versions.mix(VCFANNO_MT.out.versions) @@ -105,7 +97,7 @@ workflow ANNOTATE_MT_SNVS { emit: haplog = HAPLOGREP2_CLASSIFY_MT.out.txt // channel: [ val(meta), path(txt) ] - vcf = ch_vcf_out // channel: [ val(meta), path(vcf) ] + vcf_ann = ch_vcf_out // channel: [ val(meta), path(vcf) ] tbi = ch_tbi_out // channel: [ val(meta), path(tbi) ] report = ENSEMBLVEP_MT.out.report // channel: [ path(html) ] versions = ch_versions // channel: [ path(versions.yml) ] diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index e11b598f..d8cb9744 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -7,6 +7,7 @@ include { CALL_SNV_SENTIEON } from './variant_calling/call_snv_se include { CALL_SNV_MT } from './variant_calling/call_snv_MT' include { CALL_SNV_MT as CALL_SNV_MT_SHIFT } from './variant_calling/call_snv_MT' include { POSTPROCESS_MT_CALLS } from './variant_calling/postprocess_MT_calls' +include { GATK4_SELECTVARIANTS } from '../../modules/nf-core/gatk4/selectvariants/main' workflow CALL_SNV { take: @@ -51,6 +52,19 @@ workflow CALL_SNV { ch_case_info ) + ch_vcf = Channel.empty().mix(CALL_SNV_DEEPVARIANT.out.vcf, CALL_SNV_SENTIEON.out.vcf) + ch_tabix = Channel.empty().mix(CALL_SNV_DEEPVARIANT.out.tabix, CALL_SNV_SENTIEON.out.tabix) + + ch_vcf + .join(ch_tabix, failOnMismatch:true, failOnDuplicate:true) + .map { meta, vcf, tbi -> return [meta, vcf, tbi, []]} + .set {ch_selvar_in} + GATK4_SELECTVARIANTS(ch_selvar_in) // remove mitochondrial variants + + ch_genome_vcf = GATK4_SELECTVARIANTS.out.vcf + ch_genome_tabix = GATK4_SELECTVARIANTS.out.tbi + ch_genome_vcf_tabix = ch_genome_vcf.join(ch_genome_tabix, failOnMismatch:true, failOnDuplicate:true) + CALL_SNV_MT( ch_mt_bam_bai, ch_genome_fasta, @@ -77,19 +91,18 @@ workflow CALL_SNV { ch_case_info ) - ch_genome_vcf = Channel.empty().mix(CALL_SNV_DEEPVARIANT.out.vcf, CALL_SNV_SENTIEON.out.vcf) - ch_genome_tabix = Channel.empty().mix(CALL_SNV_DEEPVARIANT.out.tabix, CALL_SNV_SENTIEON.out.tabix) - ch_mt_vcf = POSTPROCESS_MT_CALLS.out.vcf - ch_versions = ch_versions.mix(CALL_SNV_DEEPVARIANT.out.versions) ch_versions = ch_versions.mix(CALL_SNV_SENTIEON.out.versions) ch_versions = ch_versions.mix(CALL_SNV_MT.out.versions) ch_versions = ch_versions.mix(CALL_SNV_MT_SHIFT.out.versions) ch_versions = ch_versions.mix(POSTPROCESS_MT_CALLS.out.versions) + ch_versions = ch_versions.mix(GATK4_SELECTVARIANTS.out.versions) emit: - genome_vcf = ch_genome_vcf // channel: [ val(meta), path(vcf) ] - genome_tabix = ch_genome_tabix // channel: [ val(meta), path(tbi) ] - mt_vcf = ch_mt_vcf // channel: [ val(meta), path(vcf) ] - versions = ch_versions // channel: [ path(versions.yml) ] + genome_vcf = ch_genome_vcf // channel: [ val(meta), path(vcf) ] + genome_tabix = ch_genome_tabix // channel: [ val(meta), path(tbi) ] + genome_vcf_tabix = ch_genome_vcf_tabix // channel: [ val(meta), path(vcf), path(tbi) ] + mt_vcf = POSTPROCESS_MT_CALLS.out.vcf // channel: [ val(meta), path(vcf) ] + mt_tabix = POSTPROCESS_MT_CALLS.out.tbi // channel: [ val(meta), path(vcf) ] + versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/subworkflows/local/call_structural_variants.nf b/subworkflows/local/call_structural_variants.nf index 9e749add..d614ef7c 100644 --- a/subworkflows/local/call_structural_variants.nf +++ b/subworkflows/local/call_structural_variants.nf @@ -50,7 +50,7 @@ workflow CALL_STRUCTURAL_VARIANTS { CALL_SV_MT (ch_mt_bam_bai, ch_genome_fasta) - CALL_SV_MT_SHIFT (ch_mtshift_bam_bai, ch_mtshift_fasta) +// CALL_SV_MT_SHIFT (ch_mtshift_bam_bai, ch_mtshift_fasta) //merge if (params.skip_cnv_calling) { diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 70c1422a..2a5fb036 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -94,11 +94,12 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil include { GATK4_MERGEVCFS } from '../modules/nf-core/gatk4/mergevcfs/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' include { FASTQC } from '../modules/nf-core/fastqc/main' -include { GATK4_SELECTVARIANTS } from '../modules/nf-core/gatk4/selectvariants/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' include { SMNCOPYNUMBERCALLER } from '../modules/nf-core/smncopynumbercaller/main' +include { ENSEMBLVEP_FILTERVEP as FILTERVEP_MT } from '../modules/nf-core/ensemblvep/filtervep' include { ENSEMBLVEP_FILTERVEP as FILTERVEP_SNV } from '../modules/nf-core/ensemblvep/filtervep' include { ENSEMBLVEP_FILTERVEP as FILTERVEP_SV } from '../modules/nf-core/ensemblvep/filtervep' +include { TABIX_BGZIPTABIX as BGZIPTABIX_MT } from '../modules/nf-core/tabix/bgziptabix' include { TABIX_BGZIPTABIX as BGZIPTABIX_SNV } from '../modules/nf-core/tabix/bgziptabix' include { TABIX_BGZIPTABIX as BGZIPTABIX_SV } from '../modules/nf-core/tabix/bgziptabix' @@ -108,9 +109,11 @@ include { TABIX_BGZIPTABIX as BGZIPTABIX_SV } from '../modules/nf-core/tabix include { ALIGN } from '../subworkflows/local/align' include { ANALYSE_MT } from '../subworkflows/local/analyse_MT' +include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_MT } from '../subworkflows/local/annotate_consequence_pli' include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SNV } from '../subworkflows/local/annotate_consequence_pli' include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SV } from '../subworkflows/local/annotate_consequence_pli' include { ANNOTATE_GENOME_SNVS } from '../subworkflows/local/annotate_genome_snvs' +include { ANNOTATE_MT_SNVS } from '../subworkflows/local/annotate_mt_snvs' include { ANNOTATE_STRUCTURAL_VARIANTS } from '../subworkflows/local/annotate_structural_variants' include { CALL_REPEAT_EXPANSIONS } from '../subworkflows/local/call_repeat_expansions' include { CALL_SNV } from '../subworkflows/local/call_snv' @@ -118,6 +121,7 @@ include { CALL_STRUCTURAL_VARIANTS } from '../subworkflows/local/ca include { GENS } from '../subworkflows/local/gens' include { PREPARE_REFERENCES } from '../subworkflows/local/prepare_references' include { QC_BAM } from '../subworkflows/local/qc_bam' +include { RANK_VARIANTS as RANK_VARIANTS_MT } from '../subworkflows/local/rank_variants' include { RANK_VARIANTS as RANK_VARIANTS_SNV } from '../subworkflows/local/rank_variants' include { RANK_VARIANTS as RANK_VARIANTS_SV } from '../subworkflows/local/rank_variants' include { SCATTER_GENOME } from '../subworkflows/local/scatter_genome' @@ -289,7 +293,9 @@ workflow RAREDISEASE { ch_scatter_split_intervals = ch_scatter.split_intervals ?: Channel.empty() + // // ALIGNING READS, FETCH STATS, AND MERGE. + // ALIGN ( ch_reads, ch_genome_fasta, @@ -307,7 +313,9 @@ workflow RAREDISEASE { .set { ch_mapped } ch_versions = ch_versions.mix(ALIGN.out.versions) + // // BAM QUALITY CHECK + // QC_BAM ( ch_mapped.genome_marked_bam, ch_mapped.genome_marked_bai, @@ -322,7 +330,9 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(QC_BAM.out.versions) + // // EXPANSIONHUNTER AND STRANGER + // CALL_REPEAT_EXPANSIONS ( ch_mapped.genome_bam_bai, ch_variant_catalog, @@ -332,28 +342,9 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(CALL_REPEAT_EXPANSIONS.out.versions) - // STEP 1.7: SMNCOPYNUMBERCALLER - ch_mapped.genome_bam_bai - .collect{it[1]} - .toList() - .set { ch_bam_list } - - ch_mapped.genome_bam_bai - .collect{it[2]} - .toList() - .set { ch_bai_list } - - ch_case_info - .combine(ch_bam_list) - .combine(ch_bai_list) - .set { ch_bams_bais } - - SMNCOPYNUMBERCALLER ( - ch_bams_bais - ) - ch_versions = ch_versions.mix(SMNCOPYNUMBERCALLER.out.versions) - - // STEP 2: VARIANT CALLING + // + // SNV CALLING + // CALL_SNV ( ch_mapped.genome_bam_bai, ch_mapped.mt_bam_bai, @@ -375,6 +366,9 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(CALL_SNV.out.versions) + // + // SV CALLING + // CALL_STRUCTURAL_VARIANTS ( ch_mapped.genome_marked_bam, ch_mapped.genome_marked_bai, @@ -395,29 +389,9 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(CALL_STRUCTURAL_VARIANTS.out.versions) - // ped correspondence, sex check, ancestry check - PEDDY_CHECK ( - CALL_SNV.out.genome_vcf.join(CALL_SNV.out.genome_tabix, failOnMismatch:true, failOnDuplicate:true), - ch_pedfile - ) - ch_versions = ch_versions.mix(PEDDY_CHECK.out.versions) - - // GENS - if (params.gens_switch) { - GENS ( - ch_mapped.genome_bam_bai, - CALL_SNV.out.vcf, - ch_genome_fasta, - ch_genome_fai, - file(params.gens_interval_list), - file(params.gens_pon), - file(params.gens_gnomad_pos), - ch_case_info, - ch_genome_dictionary - ) - ch_versions = ch_versions.mix(GENS.out.versions) - } - + // + // ANNOTATE STRUCTURAL VARIANTS + // if (!params.skip_sv_annotation) { ANNOTATE_STRUCTURAL_VARIANTS ( CALL_STRUCTURAL_VARIANTS.out.vcf, @@ -455,55 +429,13 @@ workflow RAREDISEASE { } - if (!params.skip_mt_analysis) { - ANALYSE_MT ( - ch_mapped.genome_bam_bai, - ch_cadd_header, - ch_cadd_resources, - ch_genome_bwaindex, - ch_genome_bwamem2index, - ch_genome_fasta, - ch_genome_fai, - ch_genome_dictionary, - ch_mt_intervals, - ch_mtshift_bwaindex, - ch_mtshift_bwamem2index, - ch_mtshift_fasta, - ch_mtshift_dictionary, - ch_mtshift_fai, - ch_mtshift_intervals, - ch_mtshift_backchain, - ch_vcfanno_resources, - ch_vcfanno_toml, - params.genome, - params.vep_cache_version, - ch_vep_cache, - ch_case_info - ) - - ch_versions = ch_versions.mix(ANALYSE_MT.out.versions) - - } - - // VARIANT ANNOTATION - + // + // ANNOTATE GENOME SNVs + // if (!params.skip_snv_annotation) { - ch_vcf = CALL_SNV.out.genome_vcf.join(CALL_SNV.out.genome_tabix, failOnMismatch:true, failOnDuplicate:true) - - if (!params.skip_mt_analysis) { - ch_vcf - .map { meta, vcf, tbi -> return [meta, vcf, tbi, []]} - .set { ch_selvar_in } - - GATK4_SELECTVARIANTS(ch_selvar_in) // remove mitochondrial variants - - ch_vcf = GATK4_SELECTVARIANTS.out.vcf.join(GATK4_SELECTVARIANTS.out.tbi, failOnMismatch:true, failOnDuplicate:true) - ch_versions = ch_versions.mix(GATK4_SELECTVARIANTS.out.versions) - } - ANNOTATE_GENOME_SNVS ( - ch_vcf, + CALL_SNV.out.genome_vcf_tabix, params.analysis_type, ch_cadd_header, ch_cadd_resources, @@ -521,18 +453,6 @@ workflow RAREDISEASE { ch_snv_annotate = ANNOTATE_GENOME_SNVS.out.vcf_ann - if (!params.skip_mt_analysis) { - - ANNOTATE_GENOME_SNVS.out.vcf_ann - .concat(ANALYSE_MT.out.vcf) - .groupTuple() - .set { ch_merged_vcf } - - GATK4_MERGEVCFS (ch_merged_vcf, ch_genome_dictionary) - ch_snv_annotate = GATK4_MERGEVCFS.out.vcf - ch_versions = ch_versions.mix(GATK4_MERGEVCFS.out.versions) - } - ANN_CSQ_PLI_SNV ( ch_snv_annotate, ch_variant_consequences @@ -558,6 +478,94 @@ workflow RAREDISEASE { } + // + // ANNOTATE MT SNVs + // + if (!params.skip_mt_annotation) { + + ANNOTATE_MT_SNVS ( + CALL_SNV.out.mt_vcf, + CALL_SNV.out.mt_tabix, + ch_cadd_header, + ch_cadd_resources, + ch_genome_fasta, + ch_vcfanno_resources, + ch_vcfanno_toml, + params.genome, + params.vep_cache_version, + ch_vep_cache, + ).set {ch_mt_annotate} + ch_versions = ch_versions.mix(ch_mt_annotate.versions) + + ANN_CSQ_PLI_MT ( + ch_mt_annotate.vcf_ann, + ch_variant_consequences + ) + ch_versions = ch_versions.mix(ANN_CSQ_PLI_MT.out.versions) + + RANK_VARIANTS_MT ( + ANN_CSQ_PLI_MT.out.vcf_ann, + ch_pedfile, + ch_reduced_penetrance, + ch_score_config_snv + ) + ch_versions = ch_versions.mix(RANK_VARIANTS_MT.out.versions) + + FILTERVEP_MT( + RANK_VARIANTS_MT.out.vcf, + ch_vep_filters + ) + ch_versions = ch_versions.mix(FILTERVEP_MT.out.versions) + + BGZIPTABIX_MT(FILTERVEP_MT.out.output) + ch_versions = ch_versions.mix(BGZIPTABIX_MT.out.versions) + + } + + // STEP 1.7: SMNCOPYNUMBERCALLER + ch_mapped.genome_bam_bai + .collect{it[1]} + .toList() + .set { ch_bam_list } + + ch_mapped.genome_bam_bai + .collect{it[2]} + .toList() + .set { ch_bai_list } + + ch_case_info + .combine(ch_bam_list) + .combine(ch_bai_list) + .set { ch_bams_bais } + + SMNCOPYNUMBERCALLER ( + ch_bams_bais + ) + ch_versions = ch_versions.mix(SMNCOPYNUMBERCALLER.out.versions) + + // ped correspondence, sex check, ancestry check + PEDDY_CHECK ( + CALL_SNV.out.genome_vcf.join(CALL_SNV.out.genome_tabix, failOnMismatch:true, failOnDuplicate:true), + ch_pedfile + ) + ch_versions = ch_versions.mix(PEDDY_CHECK.out.versions) + + // GENS + if (params.gens_switch) { + GENS ( + ch_mapped.genome_bam_bai, + CALL_SNV.out.vcf, + ch_genome_fasta, + ch_genome_fai, + file(params.gens_interval_list), + file(params.gens_pon), + file(params.gens_gnomad_pos), + ch_case_info, + ch_genome_dictionary + ) + ch_versions = ch_versions.mix(GENS.out.versions) + } + // // MODULE: Pipeline reporting // From d711b05f7babeb25292fc9c4d2b890f2eb65b6d9 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sat, 26 Aug 2023 11:55:35 +0200 Subject: [PATCH 091/387] update configs --- conf/modules/align_MT.config | 8 +- conf/modules/align_and_call_MT.config | 126 ------------------- conf/modules/analyse_MT.config | 31 ----- conf/modules/annotate_consequence_pli.config | 32 ++++- conf/modules/annotate_genome_snvs.config | 8 +- conf/modules/annotate_mt_snvs.config | 4 +- conf/modules/call_snv.config | 10 ++ conf/modules/call_snv_deepvariant.config | 14 --- conf/modules/call_snv_sentieon.config | 14 --- conf/modules/call_structural_variants.config | 4 +- conf/modules/call_sv_MT.config | 4 +- conf/modules/convert_mt_bam_to_fastq.config | 6 +- conf/modules/merge_annotate_MT.config | 88 ------------- conf/modules/postprocess_MT_calls.config | 15 ++- conf/modules/prepare_references.config | 10 +- conf/modules/rank_variants.config | 52 ++++++++ conf/modules/raredisease.config | 33 +++-- conf/test.config | 1 + conf/test_full.config | 2 +- docs/output.md | 8 +- main.nf | 1 + nextflow.config | 5 +- nextflow_schema.json | 11 +- workflows/raredisease.nf | 6 +- 24 files changed, 162 insertions(+), 331 deletions(-) delete mode 100644 conf/modules/align_and_call_MT.config delete mode 100644 conf/modules/analyse_MT.config delete mode 100644 conf/modules/merge_annotate_MT.config diff --git a/conf/modules/align_MT.config b/conf/modules/align_MT.config index a01bfc60..35d91ee3 100644 --- a/conf/modules/align_MT.config +++ b/conf/modules/align_MT.config @@ -18,13 +18,13 @@ process { withName: '.*ALIGN_MT:BWAMEM2_MEM_MT' { - ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "bwamem2" } + ext.when = { !(params.analysis_type == "wes") && params.aligner == "bwamem2" } ext.args = { "-M -K 100000000 -R ${meta.read_group}" } } withName: '.*ALIGN_MT:SENTIEON_BWAMEM_MT' { ext.args = { "-M -K 10000000 -R ${meta.read_group}" } - ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "sentieon" } + ext.when = { !(params.analysis_type == "wes") && params.aligner == "sentieon" } ext.prefix = { "${meta.id}.sorted" } } @@ -61,13 +61,13 @@ process { process { withName: '.*ALIGN_MT_SHIFT:BWAMEM2_MEM_MT' { - ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "bwamem2" } + ext.when = { !(params.analysis_type == "wes") && params.aligner == "bwamem2" } ext.args = { "-M -K 100000000 -R ${meta.read_group}" } } withName: '.*ALIGN_MT_SHIFT:SENTIEON_BWAMEM_MT' { ext.args = { "-M -K 10000000 -R ${meta.read_group}" } - ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "sentieon" } + ext.when = { !(params.analysis_type == "wes") && params.aligner == "sentieon" } ext.prefix = { "${meta.id}.sorted" } } diff --git a/conf/modules/align_and_call_MT.config b/conf/modules/align_and_call_MT.config deleted file mode 100644 index 1a2993f5..00000000 --- a/conf/modules/align_and_call_MT.config +++ /dev/null @@ -1,126 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Config file for defining DSL2 per module options and publishing paths -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Available keys to override module options: - ext.args = Additional arguments appended to command in module. - ext.args2 = Second set of arguments appended to command in module (multi-tool modules). - ext.args3 = Third set of arguments appended to command in module (multi-tool modules). - ext.prefix = File name prefix for output files. - ext.when = Conditional clause ----------------------------------------------------------------------------------------- -*/ - -// -// ANALYSE_MT:ALIGN_AND_CALL_MT -// - -process { - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT:BWAMEM2_MEM_MT' { - ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "bwamem2" } - ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT:SENTIEON_BWAMEM_MT' { - ext.args = { "-M -K 10000000 -R ${meta.read_group}" } - ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "sentieon" } - ext.prefix = { "${meta.id}.sorted" } - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT:GATK4_MERGEBAMALIGNMENT_MT' { - ext.args = '--CREATE_INDEX true --MAX_GAPS -1 --SORT_ORDER queryname --INCLUDE_SECONDARY_ALIGNMENTS false --PAIRED_RUN false --VALIDATION_STRINGENCY LENIENT' - ext.prefix = { "${meta.id}_merged" } - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT:PICARD_ADDORREPLACEREADGROUPS_MT' { - ext.args = { [ - "--VALIDATION_STRINGENCY LENIENT", - "--RGLB lib", - "--RGPL ILLUMINA", - "--RGPU barcode", - "--RGSM ${meta.id}" - ].join(' ' ).trim() } - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT:PICARD_MARKDUPLICATES_MT' { - ext.args = '--VALIDATION_STRINGENCY LENIENT --CREATE_INDEX true' - ext.prefix = { "${meta.id}_markduplicates" } - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT:SAMTOOLS_SORT_MT' { - ext.prefix = { "${meta.id}_sorted" } - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT:MT_DELETION' { - ext.args = '-s --insert-size 16000' - publishDir = [ - path: { "${params.outdir}/mt_sv" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT:GATK4_MUTECT2_MT' { - ext.args = '--mitochondria-mode TRUE' - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT:GATK4_FILTERMUTECTCALLS_MT' { - ext.prefix = { "${meta.id}_filtered" } - } -} - -// -// ANALYSE_MT:ALIGN_AND_CALL_MT_SHIFT -// - -process { - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT_SHIFT:BWAMEM2_MEM_MT' { - ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "bwamem2" } - ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT_SHIFT:SENTIEON_BWAMEM_MT' { - ext.args = { "-M -K 10000000 -R ${meta.read_group}" } - ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "sentieon" } - ext.prefix = { "${meta.id}.sorted" } - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT_SHIFT:GATK4_MERGEBAMALIGNMENT_MT' { - ext.args = '--CREATE_INDEX true --MAX_GAPS -1 --SORT_ORDER queryname --INCLUDE_SECONDARY_ALIGNMENTS false --PAIRED_RUN false --VALIDATION_STRINGENCY LENIENT' - ext.prefix = { "${meta.id}_merged_shifted" } - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT_SHIFT:PICARD_ADDORREPLACEREADGROUPS_MT' { - ext.args = { [ - "--VALIDATION_STRINGENCY LENIENT", - "--RGLB lib", - "--RGPL ${params.platform}", - "--RGPU barcode", - "--RGSM ${meta.id}" - ].join(' ' ).trim() } - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT_SHIFT:PICARD_MARKDUPLICATES_MT' { - ext.args = '--VALIDATION_STRINGENCY LENIENT --CREATE_INDEX true' - ext.prefix = { "${meta.id}_markduplicates_shifted" } - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT_SHIFT:SAMTOOLS_SORT_MT' { - ext.prefix = { "${meta.id}_sorted_shifted" } - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT_SHIFT:GATK4_MUTECT2_MT' { - ext.args = '--mitochondria-mode TRUE' - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT_SHIFT:HAPLOCHECK_MT' { - ext.prefix = { "${meta.id}_shifted" } - } - - withName: '.*ANALYSE_MT:ALIGN_AND_CALL_MT_SHIFT:GATK4_FILTERMUTECTCALLS_MT' { - ext.prefix = { "${meta.id}_filtered_shifted" } - } - -} diff --git a/conf/modules/analyse_MT.config b/conf/modules/analyse_MT.config deleted file mode 100644 index 4ee1b693..00000000 --- a/conf/modules/analyse_MT.config +++ /dev/null @@ -1,31 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Config file for defining DSL2 per module options and publishing paths -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Available keys to override module options: - ext.args = Additional arguments appended to command in module. - ext.args2 = Second set of arguments appended to command in module (multi-tool modules). - ext.args3 = Third set of arguments appended to command in module (multi-tool modules). - ext.prefix = File name prefix for output files. - ext.when = Conditional clause ----------------------------------------------------------------------------------------- -*/ - -// -// Liftover -// - -process { - withName: '.*ANALYSE_MT:.*' { - ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") } - publishDir = [ - enabled: false - ] - } -} - -process { - withName: '.*ANALYSE_MT:PICARD_LIFTOVERVCF' { - ext.prefix = { "${meta.id}_liftover" } - } -} diff --git a/conf/modules/annotate_consequence_pli.config b/conf/modules/annotate_consequence_pli.config index ca4db826..eed623a6 100644 --- a/conf/modules/annotate_consequence_pli.config +++ b/conf/modules/annotate_consequence_pli.config @@ -21,15 +21,15 @@ process { } withName: '.*ANN_CSQ_PLI_SV:ADD_MOST_SEVERE_CSQ' { - ext.prefix = { "${meta.id}_vep_csq" } + ext.prefix = { "${meta.id}_genome_vep_csq" } } withName: '.*ANN_CSQ_PLI_SV:ADD_MOST_SEVERE_PLI' { - ext.prefix = { "${meta.id}_vep_csq_pli" } + ext.prefix = { "${meta.id}_genome_vep_csq_pli" } } withName: '.*ANN_CSQ_PLI_SV:TABIX_BGZIPTABIX' { - ext.prefix = { "${meta.id}_vep_csq_pli" } + ext.prefix = { "${meta.id}_genome_vep_csq_pli" } publishDir = [ path: { "${params.outdir}/annotate_sv" }, mode: params.publish_dir_mode, @@ -64,3 +64,29 @@ process { ] } } + +process { + withName: '.*ANN_CSQ_PLI_MT:.*' { + ext.when = !params.skip_mt_annotation + publishDir = [ + enabled: false + ] + } + + withName: '.*ANN_CSQ_PLI_MT:ADD_MOST_SEVERE_CSQ' { + ext.prefix = { "${meta.id}_mt_vep_csq" } + } + + withName: '.*ANN_CSQ_PLI_MT:ADD_MOST_SEVERE_PLI' { + ext.prefix = { "${meta.id}_mt_vep_csq_pli" } + } + + withName: '.*ANN_CSQ_PLI_MT:TABIX_BGZIPTABIX' { + ext.prefix = { "${meta.id}_mt_vep_csq_pli" } + publishDir = [ + path: { "${params.outdir}/annotate_snv" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } +} diff --git a/conf/modules/annotate_genome_snvs.config b/conf/modules/annotate_genome_snvs.config index 0cbf5496..96503d82 100644 --- a/conf/modules/annotate_genome_snvs.config +++ b/conf/modules/annotate_genome_snvs.config @@ -57,7 +57,7 @@ process { ext.args = { "--euploid" } tag = {"${meta7.id}"} publishDir = [ - path: { "${params.outdir}/annotate_snv" }, + path: { "${params.outdir}/annotate_snv/genome" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -69,7 +69,7 @@ process { ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun } tag = {"${meta6.id}"} publishDir = [ - path: { "${params.outdir}/annotate_snv" }, + path: { "${params.outdir}/annotate_snv/genome" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -106,7 +106,7 @@ process { withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_CONCAT' { ext.prefix = { "${meta.id}_rohann_vcfanno_filter_vep" } publishDir = [ - path: { "${params.outdir}/annotate_snv" }, + path: { "${params.outdir}/annotate_snv/genome" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -114,7 +114,7 @@ process { withName: '.*ANNOTATE_GENOME_SNVS:TABIX_BCFTOOLS_CONCAT' { publishDir = [ - path: { "${params.outdir}/annotate_snv" }, + path: { "${params.outdir}/annotate_snv/genome" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] diff --git a/conf/modules/annotate_mt_snvs.config b/conf/modules/annotate_mt_snvs.config index 68e01af1..40101033 100644 --- a/conf/modules/annotate_mt_snvs.config +++ b/conf/modules/annotate_mt_snvs.config @@ -39,7 +39,7 @@ process { withName: '.*ANNOTATE_MT_SNVS:ZIP_TABIX_HMTNOTE' { ext.prefix = { "${meta.id}_vep_vcfanno_hmtnote_mt" } publishDir = [ - path: { "${params.outdir}/annotate_mt" }, + path: { "${params.outdir}/annotate_snv/mitochondria" }, mode: params.publish_dir_mode, pattern: "*{vcf.gz,vcf.gz.tbi}", saveAs: { filename -> filename.equals('versions.yml') ? null : filename } @@ -57,7 +57,7 @@ process { withName: '.*ANNOTATE_MT_SNVS:HAPLOGREP2_CLASSIFY_MT' { ext.prefix = { "${meta.id}_haplogrep" } publishDir = [ - path: { "${params.outdir}/annotate_mt" }, + path: { "${params.outdir}/annotate_snv/mitochondria" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] diff --git a/conf/modules/call_snv.config b/conf/modules/call_snv.config index 53cc78b4..17926228 100644 --- a/conf/modules/call_snv.config +++ b/conf/modules/call_snv.config @@ -19,4 +19,14 @@ process { ] } + withName: '.*CALL_SNV:GATK4_SELECTVARIANTS' { + ext.args = "--exclude-intervals ${params.mito_name}" + ext.prefix = { "${meta.id}_nomito" } + ext.when = { !params.skip_snv_annotation } + publishDir = [ + path: { "${params.outdir}/call_snv/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } diff --git a/conf/modules/call_snv_deepvariant.config b/conf/modules/call_snv_deepvariant.config index 0493534e..329bc15f 100644 --- a/conf/modules/call_snv_deepvariant.config +++ b/conf/modules/call_snv_deepvariant.config @@ -37,20 +37,6 @@ process { withName: '.*CALL_SNV_DEEPVARIANT:REMOVE_DUPLICATES_GL' { ext.args = '--output-type z --rm-dup none' ext.prefix = { "${meta.id}_split_rmdup" } - publishDir = [ - enabled: params.skip_mt_analysis, - path: { "${params.outdir}/call_snv" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] } - withName: '.*CALL_SNV_DEEPVARIANT:TABIX_GL' { - publishDir = [ - enabled: params.skip_mt_analysis, - path: { "${params.outdir}/call_snv" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } } diff --git a/conf/modules/call_snv_sentieon.config b/conf/modules/call_snv_sentieon.config index ec4d4480..b4443546 100644 --- a/conf/modules/call_snv_sentieon.config +++ b/conf/modules/call_snv_sentieon.config @@ -51,20 +51,6 @@ process { withName: '.*CALL_SNV:CALL_SNV_SENTIEON:REMOVE_DUPLICATES_SEN' { ext.args = '--output-type z --rm-dup none' ext.prefix = { "${meta.id}_split_rmdup" } - publishDir = [ - enabled: params.skip_mt_analysis, - path: { "${params.outdir}/call_snv" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] } - withName: '.*CALL_SNV_SENTIEON:TABIX_SEN' { - publishDir = [ - enabled: params.skip_mt_analysis, - path: { "${params.outdir}/call_snv" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } } diff --git a/conf/modules/call_structural_variants.config b/conf/modules/call_structural_variants.config index a8c10175..3a59868d 100644 --- a/conf/modules/call_structural_variants.config +++ b/conf/modules/call_structural_variants.config @@ -26,7 +26,7 @@ process { withName: '.*CALL_STRUCTURAL_VARIANTS:SVDB_MERGE' { ext.args = '--pass_only --same_order' publishDir = [ - path: { "${params.outdir}/call_sv" }, + path: { "${params.outdir}/call_sv/genome" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -34,7 +34,7 @@ process { withName: '.*CALL_STRUCTURAL_VARIANTS:TABIX_TABIX' { publishDir = [ - path: { "${params.outdir}/call_sv" }, + path: { "${params.outdir}/call_sv/genome" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] diff --git a/conf/modules/call_sv_MT.config b/conf/modules/call_sv_MT.config index 288ca425..44b0e581 100644 --- a/conf/modules/call_sv_MT.config +++ b/conf/modules/call_sv_MT.config @@ -20,7 +20,7 @@ process { withName: '.*CALL_SV_MT:MT_DELETION' { ext.args = '-s --insert-size 16000' publishDir = [ - path: { "${params.outdir}/mt_sv" }, + path: { "${params.outdir}/call_sv/mitochondria" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -28,7 +28,7 @@ process { withName: '.*CALL_SV_MT:EKLIPSE' { publishDir = [ - path: { "${params.outdir}/mt_sv" }, + path: { "${params.outdir}/call_sv/mitochondria" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] diff --git a/conf/modules/convert_mt_bam_to_fastq.config b/conf/modules/convert_mt_bam_to_fastq.config index 9a683b6e..0365c452 100644 --- a/conf/modules/convert_mt_bam_to_fastq.config +++ b/conf/modules/convert_mt_bam_to_fastq.config @@ -17,7 +17,7 @@ process { - withName: '.*ANALYSE_MT:CONVERT_MT_BAM_TO_FASTQ:GATK4_PRINTREADS_MT' { + withName: '.*ALIGN:CONVERT_MT_BAM_TO_FASTQ:GATK4_PRINTREADS_MT' { beforeScript = {"mkdir ./tmp"} ext.args = [ "-L ${params.mito_name}", @@ -26,11 +26,11 @@ process { ].join(" ").trim() } - withName: '.*ANALYSE_MT:CONVERT_MT_BAM_TO_FASTQ:GATK4_REVERTSAM_MT' { + withName: '.*ALIGN:CONVERT_MT_BAM_TO_FASTQ:GATK4_REVERTSAM_MT' { ext.args = '--OUTPUT_BY_READGROUP false --VALIDATION_STRINGENCY LENIENT --ATTRIBUTE_TO_CLEAR FT --ATTRIBUTE_TO_CLEAR CO --SORT_ORDER queryname --RESTORE_ORIGINAL_QUALITIES false' } - withName: '.*ANALYSE_MT:CONVERT_MT_BAM_TO_FASTQ:GATK4_SAMTOFASTQ_MT' { + withName: '.*ALIGN:CONVERT_MT_BAM_TO_FASTQ:GATK4_SAMTOFASTQ_MT' { ext.args = '--VALIDATION_STRINGENCY LENIENT' } } diff --git a/conf/modules/merge_annotate_MT.config b/conf/modules/merge_annotate_MT.config deleted file mode 100644 index ae2601b6..00000000 --- a/conf/modules/merge_annotate_MT.config +++ /dev/null @@ -1,88 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Config file for defining DSL2 per module options and publishing paths -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Available keys to override module options: - ext.args = Additional arguments appended to command in module. - ext.args2 = Second set of arguments appended to command in module (multi-tool modules). - ext.args3 = Third set of arguments appended to command in module (multi-tool modules). - ext.prefix = File name prefix for output files. - ext.when = Conditional clause ----------------------------------------------------------------------------------------- -*/ - -// -// Mitochondrial annotation options -// - -process { - - withName: '.*ANALYSE_MT:MERGE_ANNOTATE_MT:GATK4_MERGEVCFS_LIFT_UNLIFT_MT' { - ext.prefix = { "${meta.id}_merged" } - } - - withName: '.*ANALYSE_MT:MERGE_ANNOTATE_MT:GATK4_VARIANTFILTRATION_MT' { - ext.prefix = { "${meta.id}_filt" } - } - - withName: '.*ANALYSE_MT:MERGE_ANNOTATE_MT:SPLIT_MULTIALLELICS_MT' { - ext.args = '--output-type z --multiallelics -both' - ext.prefix = { "${meta.id}_split" } - } - - withName: '.*ANALYSE_MT:MERGE_ANNOTATE_MT:REMOVE_DUPLICATES_MT' { - ext.args = '--output-type z --rm-dup none' - ext.prefix = { "${meta.id}_split_rmdup" } - } - - withName: '.*ANALYSE_MT:MERGE_ANNOTATE_MT:BCFTOOLS_MERGE_MT' { - ext.args = '--output-type z' - ext.prefix = { "${meta.id}_merge_mt" } - } - - withName: '.*ANALYSE_MT:MERGE_ANNOTATE_MT:ENSEMBLVEP_MT' { - ext.args = [ - '--dir_plugins vep_cache/Plugins', - '--plugin LoFtool,vep_cache/LoFtool_scores.txt', - '--plugin pLI,vep_cache/pLI_values_107.txt', - '--plugin SpliceAI,snv=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', - '--plugin MaxEntScan,vep_cache/fordownload,SWA,NCSS', - '--distance 0', - '--buffer_size 20000', - '--format vcf --fork 4 --max_sv_size 248956422', - '--appris --biotype --cache --canonical --ccds --compress_output bgzip', - '--domains --exclude_predicted --force_overwrite', - '--hgvs --humdiv --no_progress --no_stats --numbers', - '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl --vcf', - '--uniprot' - ].join(' ') - } - - withName: '.*ANALYSE_MT:MERGE_ANNOTATE_MT:ZIP_TABIX_HMTNOTE' { - ext.prefix = { "${meta.id}_vep_vcfanno_hmtnote_mt" } - publishDir = [ - path: { "${params.outdir}/annotate_mt" }, - mode: params.publish_dir_mode, - pattern: "*{vcf.gz,vcf.gz.tbi}", - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*ANALYSE_MT:MERGE_ANNOTATE_MT:HMTNOTE_ANNOTATE' { - ext.prefix = { "${meta.id}_vep_vcfanno_hmtnote_mt" } - ext.args = '--offline' - publishDir = [ - enabled: false - ] - } - - withName: '.*ANALYSE_MT:MERGE_ANNOTATE_MT:HAPLOGREP2_CLASSIFY_MT' { - ext.prefix = { "${meta.id}_haplogrep" } - publishDir = [ - path: { "${params.outdir}/annotate_mt" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - -} diff --git a/conf/modules/postprocess_MT_calls.config b/conf/modules/postprocess_MT_calls.config index 3119012e..c97ea43d 100644 --- a/conf/modules/postprocess_MT_calls.config +++ b/conf/modules/postprocess_MT_calls.config @@ -37,7 +37,20 @@ process { withName: '.*POSTPROCESS_MT_CALLS:BCFTOOLS_MERGE_MT' { ext.args = '--output-type z' - ext.prefix = { "${meta.id}_merge_mt" } + ext.prefix = { "${meta.id}_mitochondria" } + publishDir = [ + path: { "${params.outdir}/call_snv/mitochondria" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*POSTPROCESS_MT_CALLS:TABIX_TABIX_MERGE' { + publishDir = [ + path: { "${params.outdir}/call_snv/mitochondria" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } } diff --git a/conf/modules/prepare_references.config b/conf/modules/prepare_references.config index 2532052d..101824ef 100644 --- a/conf/modules/prepare_references.config +++ b/conf/modules/prepare_references.config @@ -30,7 +30,7 @@ process { } withName: '.*PREPARE_REFERENCES:BWAMEM2_INDEX_MT_SHIFT' { - ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "bwamem2"} + ext.when = { !(params.analysis_type == "wes") && params.aligner == "bwamem2"} } withName: '.*PREPARE_REFERENCES:SENTIEON_BWAINDEX_GENOME' { @@ -38,7 +38,7 @@ process { } withName: '.*PREPARE_REFERENCES:SENTIEON_BWAINDEX_MT_SHIFT' { - ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes") && params.aligner == "sentieon"} + ext.when = { !(params.analysis_type == "wes") && params.aligner == "sentieon"} } withName: '.*PREPARE_REFERENCES:BWA_INDEX_GENOME' { @@ -51,11 +51,11 @@ process { withName: '.*PREPARE_REFERENCES:SAMTOOLS_EXTRACT_MT' { ext.args = { " ${params.mito_name} -o ${meta.id}_mt.fa" } - ext.when = {!params.mt_fasta && !params.skip_mt_analysis} + ext.when = {!params.mt_fasta} } withName: '.*PREPARE_REFERENCES:SAMTOOLS_FAIDX_MT_SHIFT' { - ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes")} + ext.when = { !(params.analysis_type == "wes")} } withName: '.*PREPARE_REFERENCES:GATK_SD' { @@ -67,7 +67,7 @@ process { } withName: '.*PREPARE_REFERENCES:GATK_SD_MT_SHIFT' { - ext.when = { !params.skip_mt_analysis && !(params.analysis_type == "wes")} + ext.when = { !(params.analysis_type == "wes")} } withName: '.*PREPARE_REFERENCES:GET_CHROM_SIZES' { diff --git a/conf/modules/rank_variants.config b/conf/modules/rank_variants.config index 64b7a53b..f5882da1 100644 --- a/conf/modules/rank_variants.config +++ b/conf/modules/rank_variants.config @@ -111,3 +111,55 @@ process { ] } } + +// +// Score and rank MT SNVs +// + +process { + withName: '.*RANK_VARIANTS_MT:.*' { + ext.when = !params.skip_snv_annotation + publishDir = [ + enabled: false + ] + } + + withName: '.*RANK_VARIANTS_MT:GENMOD_ANNOTATE' { + ext.args = { + (params.genome == 'GRCh37') ? '--annotate_regions --genome-build 37' : '--annotate_regions --genome-build 38' + } + } + + withName: '.*RANK_VARIANTS_MT:GENMOD_MODELS' { + ext.args = " --whole_gene " + } + + withName: '.*RANK_VARIANTS_MT:GENMOD_SCORE' { + ext.args = " --rank_results " + } + + withName: '.*RANK_VARIANTS_MT:GENMOD_COMPOUND' { + ext.prefix = { "${meta.id}_ranked_mt" } + } + + withName: '.*RANK_VARIANTS_MT:BCFTOOLS_SORT' { + ext.when = false + } + + withName: '.*RANK_VARIANTS_MT:TABIX_BGZIP' { + ext.prefix = { "${meta.id}_ranked_mt" } + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*RANK_VARIANTS_MT:TABIX_TABIX' { + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } +} diff --git a/conf/modules/raredisease.config b/conf/modules/raredisease.config index c4bab3d1..f77e8a66 100644 --- a/conf/modules/raredisease.config +++ b/conf/modules/raredisease.config @@ -40,23 +40,6 @@ process { } } -// -// Remove mitochondrial variants -// - -process { - withName: '.*RAREDISEASE:GATK4_SELECTVARIANTS' { - ext.args = "--exclude-intervals ${params.mito_name}" - ext.prefix = { "${meta.id}_nomito" } - ext.when = { !params.skip_snv_annotation } - publishDir = [ - enabled: !params.skip_mt_analysis, - path: { "${params.outdir}/call_snv" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } -} // // Merge mitochondrial and genomic vcfs @@ -134,6 +117,22 @@ process { } } +process { + withName: '.*FILTERVEP_MT' { + ext.prefix = { "${meta.id}_clinical_snv" } + ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } + } + + withName: '.*BGZIPTABIX_MT' { + ext.prefix = { "${meta.id}_clinical_snv" } + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } +} + // // Multiqc options // diff --git a/conf/test.config b/conf/test.config index 9a13845b..83edbe24 100644 --- a/conf/test.config +++ b/conf/test.config @@ -38,6 +38,7 @@ params { known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" + score_config_mt = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" score_config_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" score_config_sv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_sv.ini" svdb_query_dbs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/svdb_querydb_files.csv" diff --git a/conf/test_full.config b/conf/test_full.config index dcf02d95..587b8600 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -25,7 +25,7 @@ params { genome = 'GRCh38' // Skip annotation - skip_mt_analysis = true + skip_mt_annotation = true skip_snv_annotation = true skip_sv_annotation = true } diff --git a/docs/output.md b/docs/output.md index 6d07a3bd..6cfac5e6 100644 --- a/docs/output.md +++ b/docs/output.md @@ -98,7 +98,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - `{outputdir}/alignment/` - `*.bam|*.cram`: Alignment file in bam/cram format. - `*.bai|*.crai`: Index of the corresponding bam/cram file. - - `*.txt`: Text file containing the dedup metrics. + - `*.metrics`: Text file containing the dedup metrics.
### Quality control and reporting @@ -222,8 +222,6 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ Output files - `call_snv/` - - `_split_rmdup.vcf.gz`: normalized vcf file containing MT variants. Only published when `--skip_mt_analysis` is set. - - `_split_rmdup.vcf.gz.tbi`: index of the normalized vcf file containing MT variants. Only published when `--skip_mt_analysis` is set. - `_nomito.selectvariants.vcf.gz`: normalized vcf file containing no MT variants. - `_nomito.selectvariants.vcf.gz.tbi`: index of the vcf file containing no MT variants. @@ -237,8 +235,6 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support. Output files - `call_snv/` - - `_split_rmdup.vcf.gz`: normalized vcf file containing MT variants. Only published when `--skip_mt_analysis` is set. - - `_split_rmdup.vcf.gz.tbi`: index of the normalized vcf file containing MT variants. Only published when `--skip_mt_analysis` is set. - `_nomito.selectvariants.vcf.gz`: normalized vcf file containing no MT variants. - `_nomito.selectvariants.vcf.gz.tbi`: index of the vcf file containing no MT variants. @@ -326,7 +322,7 @@ We recommend using vcfanno to annotate SNVs with precomputed CADD scores (files Based on VEP annotations, custom scripts used by the pipeline further annotate each record with the most severe consequence, and pli scores. -> **NB**: Output files described below include mitochondrial annotations only if --skip_mt_analysis is set to true. +> **NB**: Output files described below include mitochondrial annotations only if --skip_mt_annotation is set to true.
Output files diff --git a/main.nf b/main.nf index fcce4cc7..12cdadc0 100644 --- a/main.nf +++ b/main.nf @@ -39,6 +39,7 @@ params.ploidy_model = WorkflowMain.getGenomeAttribute(params, params.reduced_penetrance = WorkflowMain.getGenomeAttribute(params, 'reduced_penetrance') params.readcount_intervals = WorkflowMain.getGenomeAttribute(params, 'readcount_intervals') params.sequence_dictionary = WorkflowMain.getGenomeAttribute(params, 'sequence_dictionary') +params.score_config_mt = WorkflowMain.getGenomeAttribute(params, 'score_config_mt') params.score_config_snv = WorkflowMain.getGenomeAttribute(params, 'score_config_snv') params.score_config_sv = WorkflowMain.getGenomeAttribute(params, 'score_config_sv') params.svdb_query_dbs = WorkflowMain.getGenomeAttribute(params, 'svdb_query_dbs') diff --git a/nextflow.config b/nextflow.config index 7c494c77..cbcdd6a6 100644 --- a/nextflow.config +++ b/nextflow.config @@ -27,7 +27,7 @@ params { skip_cnv_calling = false skip_snv_annotation = false skip_sv_annotation = false - skip_mt_analysis = false + skip_mt_annotation = false gens_switch = false cadd_resources = null platform = 'illumina' @@ -280,7 +280,6 @@ manifest { includeConfig 'conf/modules/raredisease.config' includeConfig 'conf/modules/align.config' -includeConfig 'conf/modules/analyse_MT.config' includeConfig 'conf/modules/align_MT.config' includeConfig 'conf/modules/call_snv_MT.config' includeConfig 'conf/modules/call_sv_MT.config' @@ -290,7 +289,6 @@ includeConfig 'conf/modules/postprocess_MT_calls.config' includeConfig 'conf/modules/call_structural_variants.config' includeConfig 'conf/modules/annotate_genome_snvs.config' includeConfig 'conf/modules/annotate_structural_variants.config' -includeConfig 'conf/modules/align_and_call_MT.config' includeConfig 'conf/modules/align_bwamem2.config' includeConfig 'conf/modules/align_sentieon.config' includeConfig 'conf/modules/annotate_consequence_pli.config' @@ -301,7 +299,6 @@ includeConfig 'conf/modules/call_sv_manta.config' includeConfig 'conf/modules/call_sv_tiddit.config' includeConfig 'conf/modules/convert_mt_bam_to_fastq.config' includeConfig 'conf/modules/gens.config' -includeConfig 'conf/modules/merge_annotate_MT.config' includeConfig 'conf/modules/prepare_references.config' includeConfig 'conf/modules/qc_bam.config' includeConfig 'conf/modules/rank_variants.config' diff --git a/nextflow_schema.json b/nextflow_schema.json index f5216d76..27a3365e 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -266,6 +266,13 @@ "help_text": "The saved references can be used for future pipeline runs, reducing processing times.", "fa_icon": "fas fa-save" }, + "score_config_mt": { + "type": "string", + "exists": true, + "format": "path", + "fa_icon": "fas fa-file", + "description": "MT rank model config file for genmod." + }, "score_config_snv": { "type": "string", "exists": true, @@ -388,9 +395,9 @@ "description": "Specifies whether or not to skip CNV calling.", "fa_icon": "fas fa-book" }, - "skip_mt_analysis": { + "skip_mt_annotation": { "type": "boolean", - "description": "Specifies whether or not to skip the subworkflow that analyses mitochondrial genome separate from the nuclear genome.", + "description": "Specifies whether or not to skip annotation of mitochondrial variants.", "fa_icon": "fas fa-toggle-on" }, "skip_snv_annotation": { diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 2a5fb036..327aced4 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -42,7 +42,7 @@ if (!params.skip_sv_annotation) { mandatoryParams += ["genome", "svdb_query_dbs", "vep_cache", "vep_cache_version", "score_config_sv"] } -if (!params.skip_mt_analysis) { +if (!params.skip_mt_annotation) { mandatoryParams += ["genome", "mito_name", "vcfanno_resources", "vcfanno_toml", "vep_cache_version", "vep_cache"] } @@ -250,6 +250,8 @@ workflow RAREDISEASE { : ( ch_references.readcount_intervals ?: Channel.empty() ) ch_reduced_penetrance = params.reduced_penetrance ? Channel.fromPath(params.reduced_penetrance).collect() : Channel.value([]) + ch_score_config_mt = params.score_config_mt ? Channel.fromPath(params.score_config_mt).collect() + : Channel.value([]) ch_score_config_snv = params.score_config_snv ? Channel.fromPath(params.score_config_snv).collect() : Channel.value([]) ch_score_config_sv = params.score_config_sv ? Channel.fromPath(params.score_config_sv).collect() @@ -507,7 +509,7 @@ workflow RAREDISEASE { ANN_CSQ_PLI_MT.out.vcf_ann, ch_pedfile, ch_reduced_penetrance, - ch_score_config_snv + ch_score_config_mt ) ch_versions = ch_versions.mix(RANK_VARIANTS_MT.out.versions) From 0c8c8709ad59678b6166b78944fb2a540843c5f4 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sat, 26 Aug 2023 11:59:03 +0200 Subject: [PATCH 092/387] update raredisease config --- conf/modules/raredisease.config | 17 ++++------------- 1 file changed, 4 insertions(+), 13 deletions(-) diff --git a/conf/modules/raredisease.config b/conf/modules/raredisease.config index e2300b5c..4531294c 100644 --- a/conf/modules/raredisease.config +++ b/conf/modules/raredisease.config @@ -51,17 +51,6 @@ process { } } -// -// SENTIEON_TNSCOPE_MT_CALL -// - -process { - withName: '.*SENTIEON_TNSCOPE' { - ext.args = { (params.genome == "GRCh37") ? " --interval MT " : "--interval chrM" } - ext.args2 = " --min_init_normal_lod 0,5 --min_normal_lod 2,0 --min_init_tumor_lod 1,0 --min_tumor_lod 2,8 --trim_soft_clip " - ext.when = params.variant_caller.equals("sentieon") - } -} // // Smncopynumbercaller options @@ -141,12 +130,14 @@ process { process { withName: '.*FILTERVEP_MT' { - ext.prefix = { "${meta.id}_clinical_snv" } + ext.when = !params.skip_vep_filter + ext.prefix = { "${meta.id}_clinical_mt" } ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } } withName: '.*BGZIPTABIX_MT' { - ext.prefix = { "${meta.id}_clinical_snv" } + ext.when = !params.skip_vep_filter + ext.prefix = { "${meta.id}_clinical_mt" } publishDir = [ path: { "${params.outdir}/rank_and_filter" }, mode: params.publish_dir_mode, From 37be3a76a87f11a960ceede7f8ba98ae13fd4879 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sat, 26 Aug 2023 12:11:54 +0200 Subject: [PATCH 093/387] remove skip_mt_analysis --- nextflow.config | 1 - 1 file changed, 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index b428710e..294fdbe8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -29,7 +29,6 @@ params { skip_sv_annotation = false skip_mt_annotation = false skip_vep_filter = false - skip_mt_analysis = false gens_switch = false cadd_resources = null platform = 'illumina' From 637692ef5ff88025da858a63d218bbb53972eeae Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sat, 26 Aug 2023 17:02:49 +0200 Subject: [PATCH 094/387] update output --- docs/output.md | 44 +++++++----- nextflow.config | 30 ++++---- subworkflows/local/analyse_MT.nf | 118 ------------------------------- workflows/raredisease.nf | 1 - 4 files changed, 42 insertions(+), 151 deletions(-) delete mode 100644 subworkflows/local/analyse_MT.nf diff --git a/docs/output.md b/docs/output.md index 8cc6e4ab..2a7949dc 100644 --- a/docs/output.md +++ b/docs/output.md @@ -221,7 +221,7 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ
Output files -- `call_snv/` +- `call_snv/genome` - `_nomito.selectvariants.vcf.gz`: normalized vcf file containing no MT variants. - `_nomito.selectvariants.vcf.gz.tbi`: index of the vcf file containing no MT variants. @@ -234,7 +234,7 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support.
Output files -- `call_snv/` +- `call_snv/genome` - `_nomito.selectvariants.vcf.gz`: normalized vcf file containing no MT variants. - `_nomito.selectvariants.vcf.gz.tbi`: index of the vcf file containing no MT variants. @@ -261,7 +261,7 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support.
Output files -- `call_sv/` +- `call_sv/genome` - `_sv_merge.vcf.gz`: file containing the merged variant calls. - `_sv_merge.vcf.gz.tbi`: index of the file containing the merged variant calls. @@ -322,12 +322,12 @@ We recommend using vcfanno to annotate SNVs with precomputed CADD scores (files Based on VEP annotations, custom scripts used by the pipeline further annotate each record with the most severe consequence, and pli scores. -> **NB**: Output files described below include mitochondrial annotations only if --skip_mt_annotation is set to true. +> **NB**: Output files described below do not include mitochondrial annotations only if --skip_mt_annotation is set to true.
Output files -- `annotate_snv/` +- `annotate_snv/genome` - `_rohann_vcfanno_filter_vep.vcf.gz`: file containing bcftools roh, vcfanno, and vep annotations. - `_rohann_vcfanno_filter_vep.vcf.gz.tbi`: index of the file containing bcftools roh, vcfanno, and vep annotations. - `_vep_csq_pli.vcf.gz`: file containing bcftools roh, vcfanno, vep, consequence and pli annotations. @@ -346,9 +346,9 @@ Based on VEP annotations, custom scripts used by the pipeline further annotate e
Output files -- `annotate_snv/*sites_chromograph` +- `annotate_snv/genome/*sites_chromograph` - `_rohann_vcfanno_upd_sites_.png`: file containing a plot showing upd sites across chromosomes. -- `annotate_snv/*regions_chromograph` +- `annotate_snv/genome/*regions_chromograph` - `_rohann_vcfanno_upd_regions_.png`: file containing a plot showing upd regions across chromosomes.
@@ -376,7 +376,7 @@ Based on VEP annotations, custom scripts used by the pipeline further annotate e ### Mitochondrial analysis -Mitochondrial analysis is run by default, to turn it off set `--skip_mt_analysis` to true. +Mitochondrial analysis is run by default. If you want to turn off annotations set `--skip_mt_annotation` to true. #### Alignment and variant calling @@ -388,6 +388,10 @@ The pipeline for mitochondrial variant discovery, using Mutect2, uses a high sen [MT deletion script](https://github.com/dnil/mitosign/blob/master/run_mt_del_check.sh) lists the fraction of mitochondrially aligning read pairs (per 1000) that appear discordant, as defined by an insert size of more than 1.2 kb (and less than 15 kb due to the circular nature of the genome) using samtools. +- `call_sv/mitochondria` + - `_svdbquery_vep.vcf.gz`: file containing svdb query, and vep annotations. + - `_svdbquery_vep.vcf.gz.tbi`: index of the file containing bcftools roh, vcfanno, and vep annotations. + #### Annotation: ##### HaploGrep2 @@ -397,7 +401,7 @@ The pipeline for mitochondrial variant discovery, using Mutect2, uses a high sen
Output files -- `annotate_mt/` +- `annotate_snv/mitochondria` - `_haplogrep.txt`: file containing haplogroup information.
@@ -423,9 +427,9 @@ We recommend using vcfanno to annotate SNVs with precomputed CADD scores (files
Output files -- `annotate_mt/` - - `_vep_vcfanno_mt.vcf.gz`: file containing mitochondrial annotations. - - `_vep_vcfanno_mt.vcf.gz.tbi`: index of the file containing mitochondrial annotations. +- `annotate_snv/mitochondria` + - `_vep_vcfanno_hmtnote_mt.vcf.gz`: file containing mitochondrial annotations. + - `_vep_vcfanno_hmtnote_mt.vcf.gz.tbi`: index of the file containing mitochondrial annotations.
@@ -439,12 +443,18 @@ We recommend using vcfanno to annotate SNVs with precomputed CADD scores (files Output files - `rank_and_filter/` - - `_clinical_snv.ann_filter.vcf.gz`: file containing clinically relevant SNVs. - - `_clinical_sv.ann_filter.vcf.gz`: file containing clinically relevant SVs. + - `_clinical_mt.vcf.gz`: file containing clinically relevant mitochondrial SNVs. + - `_clinical_mt.vcf.gz.tbi`: index of the file containing clinically relevant mitochondrial SNVs. + - `_clinical_snv.vcf.gz`: file containing clinically relevant SNVs. + - `_clinical_snv.vcf.gz.tbi`: index of the file containing clinically relevant SNVs. + - `_clinical_sv.vcf.gz`: file containing clinically relevant SVs. + - `_clinical_sv.vcf.gz.tbi`: index of the file containing clinically relevant SVs. + - `_ranked_mt.vcf.gz`: file containing mitochondrial SNV annotations with their rank scores. + - `_ranked_mt.vcf.gz.tbi`: index of the file containing mitochondrial SNV annotations with their rank scores. - `_ranked_snv.vcf.gz`: file containing SNV annotations with their rank scores. - - `_ranked_snv.vcf.gz.tbi`: file containing SNV annotations with their rank scores. - - `_ranked_sv.ann_filter.vcf.gz`: file containing SV annotations with their rank scores. - - `_ranked_sv.ann_filter.vcf.gz.tbi`: file containing SV annotations with their rank scores. + - `_ranked_snv.vcf.gz.tbi`: index of the file containing SNV annotations with their rank scores. + - `_ranked_sv.vcf.gz`: file containing SV annotations with their rank scores. + - `_ranked_sv.vcf.gz.tbi`: index of the file containing SV annotations with their rank scores.
diff --git a/nextflow.config b/nextflow.config index 294fdbe8..e4554ce7 100644 --- a/nextflow.config +++ b/nextflow.config @@ -281,32 +281,32 @@ manifest { includeConfig 'conf/modules/raredisease.config' includeConfig 'conf/modules/align.config' -includeConfig 'conf/modules/align_MT.config' -includeConfig 'conf/modules/call_snv_MT.config' -includeConfig 'conf/modules/call_sv_MT.config' -includeConfig 'conf/modules/annotate_mt_snvs.config' -includeConfig 'conf/modules/call_snv.config' -includeConfig 'conf/modules/postprocess_MT_calls.config' -includeConfig 'conf/modules/call_structural_variants.config' +includeConfig 'conf/modules/annotate_consequence_pli.config' includeConfig 'conf/modules/annotate_genome_snvs.config' +includeConfig 'conf/modules/annotate_mt_snvs.config' includeConfig 'conf/modules/annotate_structural_variants.config' -includeConfig 'conf/modules/align_bwamem2.config' -includeConfig 'conf/modules/align_sentieon.config' -includeConfig 'conf/modules/annotate_consequence_pli.config' includeConfig 'conf/modules/call_repeat_expansions.config' -includeConfig 'conf/modules/call_snv_deepvariant.config' -includeConfig 'conf/modules/call_snv_sentieon.config' -includeConfig 'conf/modules/call_sv_manta.config' -includeConfig 'conf/modules/call_sv_tiddit.config' +includeConfig 'conf/modules/call_snv.config' +includeConfig 'conf/modules/call_structural_variants.config' includeConfig 'conf/modules/convert_mt_bam_to_fastq.config' includeConfig 'conf/modules/gens.config' +includeConfig 'conf/modules/peddy_check.config' includeConfig 'conf/modules/prepare_references.config' includeConfig 'conf/modules/qc_bam.config' includeConfig 'conf/modules/rank_variants.config' includeConfig 'conf/modules/scatter_genome.config' +includeConfig 'conf/modules/align_MT.config' +includeConfig 'conf/modules/align_bwamem2.config' +includeConfig 'conf/modules/align_sentieon.config' includeConfig 'conf/modules/annotate_cadd.config' -includeConfig 'conf/modules/peddy_check.config' +includeConfig 'conf/modules/call_snv_MT.config' +includeConfig 'conf/modules/call_snv_deepvariant.config' +includeConfig 'conf/modules/call_snv_sentieon.config' +includeConfig 'conf/modules/call_sv_MT.config' includeConfig 'conf/modules/call_sv_germlinecnvcaller.config' +includeConfig 'conf/modules/call_sv_manta.config' +includeConfig 'conf/modules/call_sv_tiddit.config' +includeConfig 'conf/modules/postprocess_MT_calls.config' // Function to ensure that resource requirements don't go beyond // a maximum limit diff --git a/subworkflows/local/analyse_MT.nf b/subworkflows/local/analyse_MT.nf deleted file mode 100644 index af5cebff..00000000 --- a/subworkflows/local/analyse_MT.nf +++ /dev/null @@ -1,118 +0,0 @@ -// -// Analyse MT -// -include { CONVERT_MT_BAM_TO_FASTQ } from './mitochondria/convert_mt_bam_to_fastq' -include { ALIGN_AND_CALL_MT } from './mitochondria/align_and_call_MT' -include { ALIGN_AND_CALL_MT as ALIGN_AND_CALL_MT_SHIFT } from './mitochondria/align_and_call_MT' -include { PICARD_LIFTOVERVCF } from '../../modules/nf-core/picard/liftovervcf/main' -include { MERGE_ANNOTATE_MT } from './mitochondria/merge_annotate_MT' - -workflow ANALYSE_MT { - take: - ch_bam_bai // channel: [mandatory] [ val(meta), file(bam), file(bai) ] - ch_cadd_header // channel: [mandatory] [ path(txt) ] - ch_cadd_resources // channel: [mandatory] [ path(annotation) ] - ch_genome_bwa_index // channel: [mandatory] [ val(meta), path(index) ] - ch_genome_bwamem2_index // channel: [mandatory] [ val(meta), path(index) ] - ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] - ch_genome_dict // channel: [mandatory] [ val(meta), path(dict) ] - ch_mt_intervals // channel: [mandatory] [ path(interval_list) ] - ch_mtshift_bwaindex // channel: [mandatory] [ val(meta), path(index) ] - ch_mtshift_bwamem2index // channel: [mandatory] [ val(meta), path(index) ] - ch_mtshift_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_mtshift_dict // channel: [mandatory] [ val(meta), path(dict) ] - ch_mtshift_fai // channel: [mandatory] [ val(meta), path(fai) ] - ch_mtshift_intervals // channel: [mandatory] [ path(interval_list) ] - ch_mtshift_backchain // channel: [mandatory] [ val(meta), path(back_chain) ] - ch_vcfanno_resources // channel: [mandatory] [ path(resources) ] - ch_vcfanno_toml // channel: [mandatory] [ path(toml) ] - val_vep_genome // string: [mandatory] GRCh37 or GRCh38 - val_vep_cache_version // string: [mandatory] 107 - ch_vep_cache // channel: [mandatory] [ path(cache) ] - ch_case_info // channel: [mandatory] [ val(case_info) ] - - main: - ch_versions = Channel.empty() - - // PREPARING READS FOR MT ALIGNMENT - CONVERT_MT_BAM_TO_FASTQ ( - ch_bam_bai, - ch_genome_fasta, - ch_genome_fai, - ch_genome_dict - ) - - // MT ALIGNMENT AND VARIANT CALLING - ALIGN_AND_CALL_MT ( - CONVERT_MT_BAM_TO_FASTQ.out.fastq, - CONVERT_MT_BAM_TO_FASTQ.out.bam, - ch_genome_bwa_index, - ch_genome_bwamem2_index, - ch_genome_fasta, - ch_genome_dict, - ch_genome_fai, - ch_mt_intervals - ) - - ALIGN_AND_CALL_MT_SHIFT ( - CONVERT_MT_BAM_TO_FASTQ.out.fastq, - CONVERT_MT_BAM_TO_FASTQ.out.bam, - ch_mtshift_bwaindex, - ch_mtshift_bwamem2index, - ch_mtshift_fasta, - ch_mtshift_dict, - ch_mtshift_fai, - ch_mtshift_intervals - ) - - // LIFTOVER VCF FROM REFERENCE MT TO SHIFTED MT - PICARD_LIFTOVERVCF ( - ALIGN_AND_CALL_MT_SHIFT.out.vcf, - ch_genome_dict, - ch_genome_fasta, - ch_mtshift_backchain, - ) - - // MT MERGE AND ANNOTATE VARIANTS - MERGE_ANNOTATE_MT( - ALIGN_AND_CALL_MT.out.vcf, - PICARD_LIFTOVERVCF.out.vcf_lifted, - ch_cadd_header, - ch_cadd_resources, - ch_genome_fasta, - ch_genome_dict, - ch_genome_fai, - ch_vcfanno_resources, - ch_vcfanno_toml, - val_vep_genome, - val_vep_cache_version, - ch_vep_cache, - ch_case_info - ) - - ch_versions = ch_versions.mix(CONVERT_MT_BAM_TO_FASTQ.out.versions) - ch_versions = ch_versions.mix(ALIGN_AND_CALL_MT.out.versions) - ch_versions = ch_versions.mix(ALIGN_AND_CALL_MT_SHIFT.out.versions) - ch_versions = ch_versions.mix(PICARD_LIFTOVERVCF.out.versions.first()) - ch_versions = ch_versions.mix(MERGE_ANNOTATE_MT.out.versions) - - emit: - vcf = MERGE_ANNOTATE_MT.out.vcf // channel: [ val(meta), path(vcf) ] - tbi = MERGE_ANNOTATE_MT.out.tbi // channel: [ val(meta), path(tbi) ] - stats = ALIGN_AND_CALL_MT.out.stats // channel: [ val(meta), path(stats) ] - filt_stats = ALIGN_AND_CALL_MT.out.filt_stats // channel: [ val(meta), path(tsv) ] - mt_del_result = ALIGN_AND_CALL_MT.out.mt_del_result // channel: [ val(meta), path(txt) ] - stats_sh = ALIGN_AND_CALL_MT_SHIFT.out.stats // channel: [ val(meta), path(stats) ] - filt_stats_sh = ALIGN_AND_CALL_MT_SHIFT.out.filt_stats // channel: [ val(meta), path(tsv) ] - eklipse_del = ALIGN_AND_CALL_MT.out.eklipse_del // channel: [ val(meta), path(csv) ] - eklipse_genes = ALIGN_AND_CALL_MT.out.eklipse_genes // channel: [ val(meta), path(csv) ] - eklipse_circos = ALIGN_AND_CALL_MT.out.eklipse_circos // channel: [ val(meta), path(png) ] - haplog = MERGE_ANNOTATE_MT.out.haplog // channel: [ val(meta), path(txt) ] - report = MERGE_ANNOTATE_MT.out.report // channel: [ path(html) ] - txt = ALIGN_AND_CALL_MT.out.txt // channel: [ val(meta), path(txt) ] - html = ALIGN_AND_CALL_MT.out.html // channel: [ val(meta), path(html) ] - txt_sh = ALIGN_AND_CALL_MT_SHIFT.out.txt // channel: [ val(meta), path(txt) ] - html_sh = ALIGN_AND_CALL_MT_SHIFT.out.html // channel: [ val(meta), path(html) ] - versions = ch_versions // channel: [ path(versions.yml) ] -} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index cdd26dba..b1b6afb7 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -112,7 +112,6 @@ include { TABIX_BGZIPTABIX as BGZIPTABIX_SV } from '../modules/nf-core/tabix // include { ALIGN } from '../subworkflows/local/align' -include { ANALYSE_MT } from '../subworkflows/local/analyse_MT' include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_MT } from '../subworkflows/local/annotate_consequence_pli' include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SNV } from '../subworkflows/local/annotate_consequence_pli' include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SV } from '../subworkflows/local/annotate_consequence_pli' From d71250c0352a29c8a32fd14bc1420fedc417341a Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 27 Aug 2023 01:02:57 +0200 Subject: [PATCH 095/387] warn --- conf/modules/raredisease.config | 26 -------------------------- workflows/raredisease.nf | 1 - 2 files changed, 27 deletions(-) diff --git a/conf/modules/raredisease.config b/conf/modules/raredisease.config index 4531294c..5fef3537 100644 --- a/conf/modules/raredisease.config +++ b/conf/modules/raredisease.config @@ -41,16 +41,6 @@ process { } -// -// Merge mitochondrial and genomic vcfs -// - -process { - withName: '.*RAREDISEASE:GATK4_MERGEVCFS' { - ext.prefix = { "${meta.id}_mito_genome_merged" } - } -} - // // Smncopynumbercaller options @@ -75,14 +65,6 @@ process { // process { - withName: '.*RANK_VARIANTS_SNV' { - publishDir = [ - enabled: params.skip_vep_filter, - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } withName: '.*FILTERVEP_SNV' { ext.when = !params.skip_vep_filter @@ -102,14 +84,6 @@ process { } process { - withName: '.*RANK_VARIANTS_SV' { - publishDir = [ - enabled: params.skip_vep_filter, - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } withName: '.*FILTERVEP_SV' { ext.when = !params.skip_vep_filter diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index b1b6afb7..fce42b35 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -95,7 +95,6 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // MODULE: Installed directly from nf-core/modules // -include { GATK4_MERGEVCFS } from '../modules/nf-core/gatk4/mergevcfs/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' From 12bb6ae725e46b0583db295b595c69fe12c00793 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 27 Aug 2023 01:21:28 +0200 Subject: [PATCH 096/387] update output --- conf/modules/annotate_consequence_pli.config | 4 ++-- docs/output.md | 25 +++++++++++++++----- 2 files changed, 21 insertions(+), 8 deletions(-) diff --git a/conf/modules/annotate_consequence_pli.config b/conf/modules/annotate_consequence_pli.config index eed623a6..68b11951 100644 --- a/conf/modules/annotate_consequence_pli.config +++ b/conf/modules/annotate_consequence_pli.config @@ -58,7 +58,7 @@ process { withName: '.*ANN_CSQ_PLI_SNV:TABIX_BGZIPTABIX' { ext.prefix = { "${meta.id}_vep_csq_pli" } publishDir = [ - path: { "${params.outdir}/annotate_snv" }, + path: { "${params.outdir}/annotate_snv/genome" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -84,7 +84,7 @@ process { withName: '.*ANN_CSQ_PLI_MT:TABIX_BGZIPTABIX' { ext.prefix = { "${meta.id}_mt_vep_csq_pli" } publishDir = [ - path: { "${params.outdir}/annotate_snv" }, + path: { "${params.outdir}/annotate_snv/genome" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] diff --git a/docs/output.md b/docs/output.md index 2a7949dc..c977f0db 100644 --- a/docs/output.md +++ b/docs/output.md @@ -51,6 +51,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Mitochondrial analysis](#mitochondrial-analysis) - [Alignment and variant calling](#alignment-and-variant-calling) - [MT deletion script](#mt-deletion-script) + - [eKLIPse](#eklipse) - [Annotation:](#annotation-) - [HaploGrep2](#haplogrep2) - [vcfanno](#vcfanno-1) @@ -384,13 +385,25 @@ Mitochondrial analysis is run by default. If you want to turn off annotations se The pipeline for mitochondrial variant discovery, using Mutect2, uses a high sensitivity to low AF and separate alignments using opposite genome breakpoints to allow for the tracing of lineages of rare mitochondrial variants. +- `call_snv/mitochondria` + - `_mitochondria.vcf.gz`: normalized vcf file containing MT variants. + - `_mitochondria.vcf.gz.tbi`: index of the vcf file containing MT variants. + ##### MT deletion script [MT deletion script](https://github.com/dnil/mitosign/blob/master/run_mt_del_check.sh) lists the fraction of mitochondrially aligning read pairs (per 1000) that appear discordant, as defined by an insert size of more than 1.2 kb (and less than 15 kb due to the circular nature of the genome) using samtools. - `call_sv/mitochondria` - - `_svdbquery_vep.vcf.gz`: file containing svdb query, and vep annotations. - - `_svdbquery_vep.vcf.gz.tbi`: index of the file containing bcftools roh, vcfanno, and vep annotations. + - `.txt`: file containing deletions. + +##### eKLIPse + +[eKLIPse](https://github.com/dooguypapua/eKLIPse) allows the detection and quantification of large mtDNA rearrangements. + +- `call_sv/mitochondria` + - `eKLIPse_deletions.csv`: file containing all predicted deletions. + - `eKLIPse_genes.csv`: file summarizing cumulated deletions per mtDNA gene. + - `eKLIPse_.png`: circos plot. #### Annotation: @@ -445,15 +458,15 @@ We recommend using vcfanno to annotate SNVs with precomputed CADD scores (files - `rank_and_filter/` - `_clinical_mt.vcf.gz`: file containing clinically relevant mitochondrial SNVs. - `_clinical_mt.vcf.gz.tbi`: index of the file containing clinically relevant mitochondrial SNVs. - - `_clinical_snv.vcf.gz`: file containing clinically relevant SNVs. + - `_clinical_snv.vcf.gz`: file containing clinically relevant SNVs (does not include mitochondrial variants). - `_clinical_snv.vcf.gz.tbi`: index of the file containing clinically relevant SNVs. - - `_clinical_sv.vcf.gz`: file containing clinically relevant SVs. + - `_clinical_sv.vcf.gz`: file containing clinically relevant SVs (includes mitochondrial variants). - `_clinical_sv.vcf.gz.tbi`: index of the file containing clinically relevant SVs. - `_ranked_mt.vcf.gz`: file containing mitochondrial SNV annotations with their rank scores. - `_ranked_mt.vcf.gz.tbi`: index of the file containing mitochondrial SNV annotations with their rank scores. - - `_ranked_snv.vcf.gz`: file containing SNV annotations with their rank scores. + - `_ranked_snv.vcf.gz`: file containing SNV annotations with their rank scores (does not include mitochondrial variants). - `_ranked_snv.vcf.gz.tbi`: index of the file containing SNV annotations with their rank scores. - - `_ranked_sv.vcf.gz`: file containing SV annotations with their rank scores. + - `_ranked_sv.vcf.gz`: file containing SV annotations with their rank scores (includes mitochondrial variants). - `_ranked_sv.vcf.gz.tbi`: index of the file containing SV annotations with their rank scores.
From d24123734ebe929e5347a178dbbc9e5980a16fba Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 27 Aug 2023 02:00:01 +0200 Subject: [PATCH 097/387] update usage --- docs/usage.md | 42 ++++++++++++++++++++++-------------------- 1 file changed, 22 insertions(+), 20 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 24da77cd..7d1e8f12 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -20,7 +20,7 @@ Table of contents: - [6. Copy number variant calling](#6-copy-number-variant-calling) - [7. SNV annotation & Ranking](#7-snv-annotation--ranking) - [8. SV annotation & Ranking](#8-sv-annotation--ranking) - - [9. Mitochondrial analysis](#9-mitochondrial-analysis) + - [9. Mitochondrial annotation](#9-mitochondrial-annotation) - [Run the pipeline](#run-the-pipeline) - [Direct input in CLI](#direct-input-in-cli) - [Import from a config file (recommended)](#import-from-a-config-file-recommended) @@ -133,7 +133,7 @@ nf-core/raredisease consists of several tools used for various purposes. For con 5. Variant calling - Structural variants (SV) (Tiddit & Manta) 6. SNV annotation & ranking (rohcall, vcfanno, ensembl VEP, GENMOD) 7. SV annotation & ranking (SVDB query, ensembl VEP, GENMOD) -8. Mitochondrial analysis +8. Mitochondrial annotation > We have only listed the groups that require at least one input from the user. For example, the pipeline also runs SMNCopyNumberCaller, but it does not require any input other than the bam files passed by the pipeline. Hence, it is not mentioned in the list above. To know more about the tools used in the pipeline check the [README](../README.md). @@ -143,16 +143,17 @@ The mandatory and optional parameters for each category are tabulated below. ##### 1. Alignment -| Mandatory | Optional | -| ------------------- | --------------------------- | -| aligner1 | fasta_fai2 | -| fasta | bwamem22 | -| platform | known_dbsnp3 | -| | known_dbsnp_tbi3 | +| Mandatory | Optional | +| ------------------------------ | --------------------------- | +| aligner1 | fasta_fai3 | +| fasta | bwamem23 | +| platform | known_dbsnp4 | +| mito_name/mt_fasta2 | known_dbsnp_tbi4 | 1Default value is bwamem2, but if you have a valid license for Sentieon, you have the option to use Sentieon as well.
-2fasta_fai and bwamem2, if not provided by the user, will be generated by the pipeline when necessary.
-3Used only by Sentieon.
+2f If mito_name is provided, mt_fasta can be generated by the pipeline.
+3fasta_fai and bwamem2, if not provided by the user, will be generated by the pipeline when necessary.
+4Used only by Sentieon.
##### 2. QC stats from the alignment files @@ -241,16 +242,17 @@ no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sampl 1 A CSV file that describes the databases (VCFs) used by SVDB for annotating structural variants. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/svdb_querydb_files.csv). Information about the column headers can be found [here](https://github.com/J35P312/SVDB#Query). -##### 9. Mitochondrial analysis - -| Mandatory | Optional | -| ----------------- | -------- | -| genome | | -| mito_name | | -| vcfanno_resources | | -| vcfanno_toml | | -| vep_cache_version | | -| vep_cache | | +##### 9. Mitochondrial annotation + +| Mandatory | Optional | +| ----------------- | ----------- | +| genome | vep_filters | +| mito_name | | +| vcfanno_resources | | +| vcfanno_toml | | +| vep_cache_version | | +| vep_cache | | +| score_config_mt | | #### Run the pipeline From 93b47320e3190a9e3b8c929ebb0fd8182bab8bb5 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 4 Sep 2023 09:10:55 +0200 Subject: [PATCH 098/387] remove duplicate probands and children in meta --- workflows/raredisease.nf | 7 +++++-- 1 file changed, 5 insertions(+), 2 deletions(-) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 1d1d8ddd..233699f4 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -647,13 +647,16 @@ def create_case_channel(List rows) { mother = item.maternal } } + println(probands) case_info.father = father case_info.mother = mother - case_info.probands = probands - case_info.upd_children = upd_children + case_info.probands = probands.unique() + case_info.upd_children = upd_children.unique() case_info.id = rows[0].case_id + println(case_info) + return case_info } From 06f717c526e353da3570f777b9b80f69591d6d26 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 4 Sep 2023 09:14:07 +0200 Subject: [PATCH 099/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index b834f2fe..01f4cca1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -31,6 +31,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Make target bed file optional for WGS mode (Issue [#375](https://github.com/nf-core/raredisease/issues/375)) [#395](https://github.com/nf-core/raredisease/pull/395) - Added constraints to block the pipeline from running CollectWgsMetrics on WES samples [#396](https://github.com/nf-core/raredisease/pull/396) - Updated modules from nf-core [#412](https://github.com/nf-core/raredisease/pull/412) +- If present, remove duplicate entries in probands and upd_children in the meta. [#420](https://github.com/nf-core/raredisease/pull/420) ### `Updated` From 32418865a1ee0d93fa9ccb8cad0735ed5ca189ce Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 4 Sep 2023 09:18:37 +0200 Subject: [PATCH 100/387] Update raredisease.nf --- workflows/raredisease.nf | 3 --- 1 file changed, 3 deletions(-) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 233699f4..a3e1050d 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -647,7 +647,6 @@ def create_case_channel(List rows) { mother = item.maternal } } - println(probands) case_info.father = father case_info.mother = mother @@ -655,8 +654,6 @@ def create_case_channel(List rows) { case_info.upd_children = upd_children.unique() case_info.id = rows[0].case_id - println(case_info) - return case_info } From e0d7007f7c0cf53f5cc6e1a562cef6949ca176d8 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 4 Sep 2023 13:54:42 +0200 Subject: [PATCH 101/387] switch from combine to join --- subworkflows/local/annotate_snvs.nf | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/subworkflows/local/annotate_snvs.nf b/subworkflows/local/annotate_snvs.nf index bd5fdfb8..dbf62822 100644 --- a/subworkflows/local/annotate_snvs.nf +++ b/subworkflows/local/annotate_snvs.nf @@ -102,12 +102,12 @@ workflow ANNOTATE_SNVS { // If CADD is run, pick CADD output as input for VEP else pass selectvariants output to VEP. GATK4_SELECTVARIANTS.out.vcf - .combine(ANNOTATE_CADD.out.vcf.ifEmpty("null")) // If CADD is not run then this channel will be empty, so assign a default value to allow filtering with branch operator - .branch { it -> // If CADD is run, then "it" will be [[meta],selvar.vcf,[meta],cadd.vcf], else [[meta],selvar.vcf,null] - selvar: it[2].equals("null") + .join(ANNOTATE_CADD.out.vcf, remainder: true) // If CADD is not run then the third element in this channel will be `null` + .branch { it -> // If CADD is run, then "it" will be [[meta],selvar.vcf,cadd.vcf], else [[meta],selvar.vcf,null] + selvar: it[2].equals(null) return [it[0], it[1]] - cadd: !(it[2].equals("null")) - return [it[2], it[3]] + cadd: !(it[2].equals(null)) + return [it[0], it[2]] } .set { ch_for_mix } From 1ac1a4831054195db35bb2de06ce881cb0e6ad64 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 4 Sep 2023 13:58:10 +0200 Subject: [PATCH 102/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 01f4cca1..bb277017 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -32,6 +32,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Added constraints to block the pipeline from running CollectWgsMetrics on WES samples [#396](https://github.com/nf-core/raredisease/pull/396) - Updated modules from nf-core [#412](https://github.com/nf-core/raredisease/pull/412) - If present, remove duplicate entries in probands and upd_children in the meta. [#420](https://github.com/nf-core/raredisease/pull/420) +- Fixes vep starting as many instances as the square of the number of scatters. [#405](https://github.com/nf-core/raredisease/pull/405) ### `Updated` From c1640fc151dbde9bbd7f1785899c9c3e1fa816dc Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 5 Sep 2023 09:01:47 +0200 Subject: [PATCH 103/387] update headers --- subworkflows/local/align.nf | 4 +++- subworkflows/local/alignment/align_MT.nf | 1 + subworkflows/local/annotate_mt_snvs.nf | 15 ++++----------- subworkflows/local/call_structural_variants.nf | 1 + subworkflows/local/variant_calling/call_snv_MT.nf | 2 -- 5 files changed, 9 insertions(+), 14 deletions(-) diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index a705f16e..e7314f2a 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -88,7 +88,9 @@ workflow ALIGN { ch_versions = Channel.empty().mix(ALIGN_BWAMEM2.out.versions, ALIGN_SENTIEON.out.versions, ALIGN_MT.out.versions, - ALIGN_MT_SHIFT.out.versions) + ALIGN_MT_SHIFT.out.versions, + CONVERT_MT_BAM_TO_FASTQ.out.versions, + SAMTOOLS_VIEW.out.versions) emit: genome_marked_bam = ch_genome_marked_bam // channel: [ val(meta), path(bam) ] diff --git a/subworkflows/local/alignment/align_MT.nf b/subworkflows/local/alignment/align_MT.nf index 17ecc362..dec73e67 100644 --- a/subworkflows/local/alignment/align_MT.nf +++ b/subworkflows/local/alignment/align_MT.nf @@ -43,6 +43,7 @@ workflow ALIGN_MT { SAMTOOLS_INDEX_MT(SAMTOOLS_SORT_MT.out.bam) ch_versions = ch_versions.mix(BWAMEM2_MEM_MT.out.versions.first()) + ch_versions = ch_versions.mix(SENTIEON_BWAMEM_MT.out.versions.first()) ch_versions = ch_versions.mix(GATK4_MERGEBAMALIGNMENT_MT.out.versions.first()) ch_versions = ch_versions.mix(PICARD_ADDORREPLACEREADGROUPS_MT.out.versions.first()) ch_versions = ch_versions.mix(PICARD_MARKDUPLICATES_MT.out.versions.first()) diff --git a/subworkflows/local/annotate_mt_snvs.nf b/subworkflows/local/annotate_mt_snvs.nf index d6ce5659..5a6b65b5 100644 --- a/subworkflows/local/annotate_mt_snvs.nf +++ b/subworkflows/local/annotate_mt_snvs.nf @@ -1,16 +1,8 @@ // -// Merge and annotate MT +// Annotate MT // -include { GATK4_MERGEVCFS as GATK4_MERGEVCFS_LIFT_UNLIFT_MT } from '../../modules/nf-core/gatk4/mergevcfs/main' -include { GATK4_VARIANTFILTRATION as GATK4_VARIANTFILTRATION_MT } from '../../modules/nf-core/gatk4/variantfiltration/main' -include { BCFTOOLS_NORM as SPLIT_MULTIALLELICS_MT } from '../../modules/nf-core/bcftools/norm/main' include { TABIX_TABIX as TABIX_TABIX_MT } from '../../modules/nf-core/tabix/tabix/main' -include { BCFTOOLS_NORM as REMOVE_DUPLICATES_MT } from '../../modules/nf-core/bcftools/norm/main' -include { TABIX_TABIX as TABIX_TABIX_MT2 } from '../../modules/nf-core/tabix/tabix/main' -include { BCFTOOLS_MERGE as BCFTOOLS_MERGE_MT } from '../../modules/nf-core/bcftools/merge/main' -include { TABIX_TABIX as TABIX_TABIX_MERGE } from '../../modules/nf-core/tabix/tabix/main' -include { TABIX_TABIX as TABIX_TABIX_MT3 } from '../../modules/nf-core/tabix/tabix/main' include { ENSEMBLVEP as ENSEMBLVEP_MT } from '../../modules/local/ensemblvep/main' include { HAPLOGREP2_CLASSIFY as HAPLOGREP2_CLASSIFY_MT } from '../../modules/nf-core/haplogrep2/classify/main' include { VCFANNO as VCFANNO_MT } from '../../modules/nf-core/vcfanno/main' @@ -66,9 +58,9 @@ workflow ANNOTATE_MT_SNVS { ) // Running vcfanno - TABIX_TABIX_MT3(ENSEMBLVEP_MT.out.vcf_gz) + TABIX_TABIX_MT(ENSEMBLVEP_MT.out.vcf_gz) ENSEMBLVEP_MT.out.vcf_gz - .join(TABIX_TABIX_MT3.out.tbi, failOnMismatch:true, failOnDuplicate:true) + .join(TABIX_TABIX_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) .map { meta, vcf, tbi -> return [meta, vcf, tbi, []]} .set { ch_in_vcfanno } @@ -91,6 +83,7 @@ workflow ANNOTATE_MT_SNVS { ch_versions = ch_versions.mix(ANNOTATE_CADD.out.versions) ch_versions = ch_versions.mix(ENSEMBLVEP_MT.out.versions) + ch_versions = ch_versions.mix(TABIX_TABIX_MT.out.versions) ch_versions = ch_versions.mix(VCFANNO_MT.out.versions) ch_versions = ch_versions.mix(HMTNOTE_ANNOTATE.out.versions) ch_versions = ch_versions.mix(HAPLOGREP2_CLASSIFY_MT.out.versions) diff --git a/subworkflows/local/call_structural_variants.nf b/subworkflows/local/call_structural_variants.nf index d614ef7c..d0846e6e 100644 --- a/subworkflows/local/call_structural_variants.nf +++ b/subworkflows/local/call_structural_variants.nf @@ -75,6 +75,7 @@ workflow CALL_STRUCTURAL_VARIANTS { TABIX_TABIX (SVDB_MERGE.out.vcf) ch_versions = ch_versions.mix(CALL_SV_MANTA.out.versions) + ch_versions = ch_versions.mix(CALL_SV_MT.out.versions) ch_versions = ch_versions.mix(CALL_SV_TIDDIT.out.versions) ch_versions = ch_versions.mix(CALL_SV_GERMLINECNVCALLER.out.versions) ch_versions = ch_versions.mix(TABIX_TABIX.out.versions) diff --git a/subworkflows/local/variant_calling/call_snv_MT.nf b/subworkflows/local/variant_calling/call_snv_MT.nf index e91718ad..d55d1965 100644 --- a/subworkflows/local/variant_calling/call_snv_MT.nf +++ b/subworkflows/local/variant_calling/call_snv_MT.nf @@ -5,8 +5,6 @@ include { HAPLOCHECK as HAPLOCHECK_MT } from '../../../modules/nf-core/haplocheck/main' include { GATK4_MUTECT2 as GATK4_MUTECT2_MT } from '../../../modules/nf-core/gatk4/mutect2/main' include { GATK4_FILTERMUTECTCALLS as GATK4_FILTERMUTECTCALLS_MT } from '../../../modules/nf-core/gatk4/filtermutectcalls/main' -include { MT_DELETION } from '../../../modules/local/mt_deletion_script' -include { EKLIPSE as EKLIPSE_MT } from '../../../modules/nf-core/eklipse/main' workflow CALL_SNV_MT { take: From fd9c26fadfc9075b25c5ef73b62c7c0188c13153 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 5 Sep 2023 09:10:23 +0200 Subject: [PATCH 104/387] update changelog --- CHANGELOG.md | 3 +++ 1 file changed, 3 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 01f4cca1..6ef40309 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -22,6 +22,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Installed the nf-core version of the sentieon/dnamodelapply module [#403](https://github.com/nf-core/raredisease/pull/403) - Installed the nf-core version of the sentieon/wgsmetricsalgo module [#404](https://github.com/nf-core/raredisease/pull/404) - Installed the nf-core version of the sentieon/dnascope module [#406](https://github.com/nf-core/raredisease/pull/406) +- Breaks down mitochondrial analysis workflow into smaller subworkflows that are more modular [#419](https://github.com/nf-core/raredisease/pull/419) +- Replaced the parameter skip_mt_analysis which was used to turn on/off the mitochondrial workflow [#419](https://github.com/nf-core/raredisease/pull/419) +- Adds a new parameter skip_mt_annotation which can be used to turn on/off annotation and ranking for mitochondrial SNVs [#419](https://github.com/nf-core/raredisease/pull/419) ### `Fixed` From 13eab83930e6089e3a2650ab7b62af98cb302209 Mon Sep 17 00:00:00 2001 From: asp8200 Date: Tue, 5 Sep 2023 14:50:31 +0000 Subject: [PATCH 105/387] Updating sentieon-dnascope module. Replacing parameter pcr_amplification with sentieon_dnascope_pcr_indel_model --- conf/modules/annotate_snvs.config | 2 +- conf/modules/call_snv_sentieon.config | 7 +- conf/modules/peddy_check.config | 2 +- modules.json | 2 +- modules/nf-core/sentieon/dnascope/main.nf | 59 +++++++----- modules/nf-core/sentieon/dnascope/meta.yml | 94 +++++++++++-------- nextflow.config | 12 +-- nextflow_schema.json | 11 ++- subworkflows/local/call_snv.nf | 6 +- .../variant_calling/call_snv_sentieon.nf | 37 +++++--- workflows/raredisease.nf | 3 +- 11 files changed, 140 insertions(+), 95 deletions(-) diff --git a/conf/modules/annotate_snvs.config b/conf/modules/annotate_snvs.config index 093e94a1..6eb9fdda 100644 --- a/conf/modules/annotate_snvs.config +++ b/conf/modules/annotate_snvs.config @@ -66,7 +66,7 @@ process { withName: '.*ANNOTATE_SNVS:CHROMOGRAPH_REGIONS' { ext.prefix = { "${meta6.id}_rohann_vcfanno_upd_regions_chromograph" } ext.args = { '--euploid' } - ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun } + ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample', 'test_sentieon']).size() >= 1) || workflow.stubRun } tag = {"${meta6.id}"} publishDir = [ path: { "${params.outdir}/annotate_snv" }, diff --git a/conf/modules/call_snv_sentieon.config b/conf/modules/call_snv_sentieon.config index ec4d4480..6ae12dea 100644 --- a/conf/modules/call_snv_sentieon.config +++ b/conf/modules/call_snv_sentieon.config @@ -23,10 +23,9 @@ process { withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SENTIEON_DNASCOPE' { ext.prefix = { "${meta.id}_dnascope" } - ext.args2 = { [ - params.variant_type ? "--var_type ${params.variant_type}" : '', - params.pcr_amplification ? '' : "--pcr_indel_model NONE" - ].join(" ") } + ext.args2 = { + params.variant_type ? "--var_type ${params.variant_type}" : '' + } } withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SENTIEON_DNAMODELAPPLY' { diff --git a/conf/modules/peddy_check.config b/conf/modules/peddy_check.config index fcd75a21..48917af1 100644 --- a/conf/modules/peddy_check.config +++ b/conf/modules/peddy_check.config @@ -18,7 +18,7 @@ process { withName: '.*:PEDDY_CHECK:PEDDY' { // Peddy needs a bigger test set in order to run so we skip it for the two test profiles - ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun } + ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample', 'test_sentieon']).size() >= 1) || workflow.stubRun } publishDir = [ path: { "${params.outdir}/peddy_check" }, mode: params.publish_dir_mode, diff --git a/modules.json b/modules.json index 2109f33a..6416e6a1 100644 --- a/modules.json +++ b/modules.json @@ -377,7 +377,7 @@ }, "sentieon/dnascope": { "branch": "master", - "git_sha": "127edadc279e19da093fdd513926c6cdee82c306", + "git_sha": "4fb6fdc8046ec09cd30f92a2a252e9a0ba4a6309", "installed_by": ["modules"] }, "sentieon/readwriter": { diff --git a/modules/nf-core/sentieon/dnascope/main.nf b/modules/nf-core/sentieon/dnascope/main.nf index acc0ca7b..6be42a17 100644 --- a/modules/nf-core/sentieon/dnascope/main.nf +++ b/modules/nf-core/sentieon/dnascope/main.nf @@ -8,18 +8,22 @@ process SENTIEON_DNASCOPE { container 'nf-core/sentieon:202112.06' input: - tuple val(meta), path(bam), path(bai) + tuple val(meta), path(bam), path(bai), path(intervals) tuple val(meta2), path(fasta) tuple val(meta3), path(fai) tuple val(meta4), path(dbsnp) tuple val(meta5), path(dbsnp_tbi) - tuple val(meta6), path(call_interval) - tuple val(meta7), path(ml_model) + tuple val(meta6), path(ml_model) + val(pcr_indel_model) + val(emit_vcf) + val(emit_gvcf) output: - tuple val(meta), path("*.vcf.gz") , emit: vcf - tuple val(meta), path("*.vcf.gz.tbi") , emit: index - path "versions.yml" , emit: versions + tuple val(meta), path("*.unfiltered.vcf.gz") , optional:true, emit: vcf // added the substring ".unfiltered" in the filename of the vcf-files since without that the g.vcf.gz-files were ending up in the vcf-channel + tuple val(meta), path("*.unfiltered.vcf.gz.tbi"), optional:true, emit: vcf_tbi + tuple val(meta), path("*.g.vcf.gz") , optional:true, emit: gvcf // these output-files have to have the extension ".vcf.gz", otherwise the subsequent GATK-MergeVCFs will fail. + tuple val(meta), path("*.g.vcf.gz.tbi") , optional:true, emit: gvcf_tbi + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -29,14 +33,27 @@ process SENTIEON_DNASCOPE { if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." } - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - def interval = call_interval ? "--interval ${call_interval}" : '' - def dbsnp_str = dbsnp ? "-d ${dbsnp}" : '' - def model = ml_model ? "--model ${ml_model}" : '' - def prefix = task.ext.prefix ?: "${meta.id}" + def args = task.ext.args ?: '' // options for the driver + def args2 = task.ext.args2 ?: '' // options for the vcf generation + def args3 = task.ext.args3 ?: '' // options for the gvcf generation + def interval = intervals ? "--interval ${intervals}" : '' + def dbsnp_cmd = dbsnp ? "-d ${dbsnp}" : '' + def model_cmd = ml_model ? " --model ${ml_model}" : '' + def pcr_indel_model_cmd = pcr_indel_model ? " --pcr_indel_model ${pcr_indel_model}" : '' + def prefix = task.ext.prefix ?: "${meta.id}" def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: '' def sentieon_auth_data_base64 = task.ext.sentieon_auth_data_base64 ?: '' + def vcf_cmd = "" + def gvcf_cmd = "" + def base_cmd = '--algo DNAscope ' + dbsnp_cmd + ' ' + + if (emit_vcf) { // emit_vcf can be the empty string, 'variant', 'confident' or 'all' but NOT 'gvcf' + vcf_cmd = base_cmd + args2 + ' ' + model_cmd + pcr_indel_model_cmd + ' --emit_mode ' + emit_vcf + ' ' + prefix + '.unfiltered.vcf.gz' + } + + if (emit_gvcf) { // emit_gvcf can be either true or false + gvcf_cmd = base_cmd + args3 + ' ' + model_cmd + pcr_indel_model_cmd + ' --emit_mode gvcf ' + prefix + '.g.vcf.gz' + } """ if [ "\${#SENTIEON_LICENSE_BASE64}" -lt "1500" ]; then # If the string SENTIEON_LICENSE_BASE64 is short, then it is an encrypted url. @@ -55,17 +72,7 @@ process SENTIEON_DNASCOPE { echo "Decoded and exported Sentieon test-license system environment variables" fi - sentieon driver \\ - -t $task.cpus \\ - -r $fasta \\ - $args \\ - $interval \\ - -i $bam \\ - --algo DNAscope \\ - $dbsnp_str \\ - $args2 \\ - $model \\ - ${prefix}.vcf.gz + sentieon driver $args -r $fasta -t $task.cpus -i $bam $interval $vcf_cmd $gvcf_cmd cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -80,8 +87,10 @@ process SENTIEON_DNASCOPE { error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." } """ - touch ${prefix}.vcf.gz - touch ${prefix}.vcf.gz.tbi + touch ${prefix}.unfiltered.vcf.gz + touch ${prefix}.unfiltered.vcf.gz.tbi + touch ${prefix}.g.vcf.gz + touch ${prefix}.g.vcf.gz.tbi cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/sentieon/dnascope/meta.yml b/modules/nf-core/sentieon/dnascope/meta.yml index 82154b38..34e0b97b 100644 --- a/modules/nf-core/sentieon/dnascope/meta.yml +++ b/modules/nf-core/sentieon/dnascope/meta.yml @@ -17,36 +17,6 @@ input: description: | Groovy Map containing sample information. e.g. [ id:'test', single_end:false ] - - meta2: - type: map - description: | - Groovy Map containing reference information. - e.g. [ id:'test' ] - - meta3: - type: map - description: | - Groovy Map containing reference information. - e.g. [ id:'test' ] - - meta4: - type: map - description: | - Groovy Map containing reference information. - e.g. [ id:'test' ] - - meta5: - type: map - description: | - Groovy Map containing reference information. - e.g. [ id:'test' ] - - meta6: - type: map - description: | - Groovy Map containing reference information. - e.g. [ id:'test' ] - - meta7: - type: map - description: | - Groovy Map containing reference information. - e.g. [ id:'test' ] - bam: type: file description: BAM file. @@ -55,31 +25,69 @@ input: type: file description: BAI file pattern: "*.bai" + - intervals: + type: file + description: bed or interval_list file containing interval in the reference that will be used in the analysis + pattern: "*.{bed,interval_list}" + - meta2: + type: map + description: | + Groovy Map containing meta information for fasta. - fasta: type: file description: Genome fasta file pattern: "*.{fa,fasta}" + - meta3: + type: map + description: | + Groovy Map containing meta information for fasta index. - fai: type: file description: Index of the genome fasta file pattern: "*.fai" + - meta4: + type: map + description: | + Groovy Map containing meta information for dbsnp. - dbsnp: type: file description: Single Nucleotide Polymorphism database (dbSNP) file pattern: "*.vcf.gz" + - meta5: + type: map + description: | + Groovy Map containing meta information for dbsnp_tbi. - dbsnp_tbi: type: file description: Index of the Single Nucleotide Polymorphism database (dbSNP) file pattern: "*.vcf.gz.tbi" - - call_interval: + - meta6: + type: map + description: | + Groovy Map containing meta information for machine learning model for Dnascope. + - ml_model: type: file - description: bed or interval_list file containing interval in the reference that will be used in the analysis - pattern: "*.{bed,interval_list}" + description: machine learning model file + pattern: "*.model" - ml_model: type: file description: machine learning model file pattern: "*.model" - + - pcr_indel_model: + type: string + description: | + Controls the option pcr_indel_model for Dnascope. + The possible options are "NONE" (used for PCR free samples), and "HOSTILE", "AGGRESSIVE" and "CONSERVATIVE". + See Sentieons documentation for further explanation. + - emit_vcf: + type: string + description: | + Controls the vcf output from Dnascope. + Possible options are "all", "confident" and "variant". + See Sentieons documentation for further explanation. + - emit_gvcf: + type: boolean + description: If true, the haplotyper will output a gvcf output: - meta: type: map @@ -88,12 +96,20 @@ output: e.g. [ id:'test', single_end:false ] - vcf: type: file - description: VCF file - pattern: "*.{vcf.gz}" - - index: + description: Compressed VCF file + pattern: "*.unfiltered.vcf.gz" + - vcf_tbi: type: file - description: Index of the VCF file - pattern: "*.vcf.gz.tbi" + description: Index of VCF file + pattern: "*.unfiltered.vcf.gz.tbi" + - gvcf: + type: file + description: Compressed GVCF file + pattern: "*.g.vcf.gz" + - gvcf_tbi: + type: file + description: Index of GVCF file + pattern: "*.g.vcf.gz.tbi" - versions: type: file description: File containing software versions diff --git a/nextflow.config b/nextflow.config index bd904b04..2db5bcb3 100644 --- a/nextflow.config +++ b/nextflow.config @@ -44,14 +44,14 @@ params { ml_model = '' // Dnascope SNV calling - pcr_amplification = false - variant_type = 'snp,indel' + sentieon_dnascope_pcr_indel_model = 'CONSERVATIVE' + variant_type = 'snp,indel' // MultiQC options - multiqc_config = null - multiqc_title = null - multiqc_logo = null - max_multiqc_email_size = '25.MB' + multiqc_config = null + multiqc_title = null + multiqc_logo = null + max_multiqc_email_size = '25.MB' multiqc_methods_description = null // Boilerplate options diff --git a/nextflow_schema.json b/nextflow_schema.json index af85e653..d32b393d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -442,10 +442,13 @@ "fa_icon": "fas fa-align-center", "description": "Interval in the reference that will be used in the software" }, - "pcr_amplification": { - "type": "boolean", - "description": "Indicates whether the sample library is amplified using PCR or not. Set to false for PCR Free samples.", - "fa_icon": "fas fa-toggle-on" + "sentieon_dnascope_pcr_indel_model": { + "type": "string", + "default": "CONSERVATIVE", + "fa_icon": "fas fa-bacon", + "description": "Option for selecting the PCR indel model used by Sentieon Dnascope.", + "help_text": "PCR indel model used to weed out false positive indels more or less aggressively. The possible MODELs are: NONE (used for PCR free samples), and HOSTILE, AGGRESSIVE and CONSERVATIVE, in order of decreasing aggressiveness. The default value is CONSERVATIVE.", + "pattern": "^(NONE|HOSTILE|AGGRESSIVE|CONSERVATIVE)(? [meta, bam, bai, interval] + } - ch_dnamodelapply_in = SENTIEON_DNASCOPE.out.vcf.join(SENTIEON_DNASCOPE.out.index) + SENTIEON_DNASCOPE( + bam_bai_intervals, + ch_genome_fasta, + ch_genome_fai, + ch_dbsnp, + ch_dbsnp_index, + ch_ml_model, + ch_pcr_indel_model, + 'VARIANT', + false) + + ch_dnamodelapply_in = SENTIEON_DNASCOPE.out.vcf.join(SENTIEON_DNASCOPE.out.vcf_tbi) SENTIEON_DNAMODELAPPLY ( ch_dnamodelapply_in, ch_genome_fasta, ch_genome_fai, ch_ml_model ) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index a3e1050d..3499344e 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -360,7 +360,8 @@ workflow RAREDISEASE { ch_dbsnp_tbi, ch_call_interval, ch_ml_model, - ch_case_info + ch_case_info, + Channel.value(params.sentieon_dnascope_pcr_indel_model) ) ch_versions = ch_versions.mix(CALL_SNV.out.versions) From 2a548e9b594fda268a1889905bef83aa15f834bf Mon Sep 17 00:00:00 2001 From: Anders Sune Pedersen <37172585+asp8200@users.noreply.github.com> Date: Thu, 7 Sep 2023 11:31:49 +0200 Subject: [PATCH 106/387] Update subworkflows/local/call_snv.nf Co-authored-by: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> --- subworkflows/local/call_snv.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index 1f143610..2ea9f627 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -17,7 +17,7 @@ workflow CALL_SNV { ch_call_interval // channel: [mandatory] [ path(intervals) ] ch_ml_model // channel: [mandatory] [ path(model) ] ch_case_info // channel: [mandatory] [ val(case_info) ] - ch_pcr_indel_model // channel: [mandatory] [ val(sentieon_dnascope_pcr_indel_model) ] + ch_pcr_indel_model // channel: [optional] [ val(sentieon_dnascope_pcr_indel_model) ] main: ch_versions = Channel.empty() From e86782954987e078a6ed3aa20dd87ad0c144ee2d Mon Sep 17 00:00:00 2001 From: Anders Sune Pedersen <37172585+asp8200@users.noreply.github.com> Date: Thu, 7 Sep 2023 11:31:58 +0200 Subject: [PATCH 107/387] Update subworkflows/local/variant_calling/call_snv_sentieon.nf Co-authored-by: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> --- subworkflows/local/variant_calling/call_snv_sentieon.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/subworkflows/local/variant_calling/call_snv_sentieon.nf b/subworkflows/local/variant_calling/call_snv_sentieon.nf index aa736ee9..a9f5d4f8 100644 --- a/subworkflows/local/variant_calling/call_snv_sentieon.nf +++ b/subworkflows/local/variant_calling/call_snv_sentieon.nf @@ -22,7 +22,8 @@ workflow CALL_SNV_SENTIEON { ch_call_interval // channel: [mandatory] [ val(meta), path(interval) ] ch_ml_model // channel: [mandatory] [ val(meta), path(model) ] ch_case_info // channel: [mandatory] [ val(case_info) ] - ch_pcr_indel_model // channel: [mandatory] [ val(sentieon_dnascope_pcr_indel_model) ] + ch_pcr_indel_model // channel: [optional] [ val(sentieon_dnascope_pcr_indel_model) ] + main: ch_versions = Channel.empty() From 8ca4f8d83e3ccdcec392eebd57b45af5e8faaa42 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 13 Sep 2023 13:21:03 +0200 Subject: [PATCH 108/387] Lucia's comment --- docs/usage.md | 7 ++++--- 1 file changed, 4 insertions(+), 3 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 7d1e8f12..6ce21435 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -131,9 +131,10 @@ nf-core/raredisease consists of several tools used for various purposes. For con 3. Repeat expansions (ExpansionsHunter & Stranger) 4. Variant calling - SNV (DeepVariant/Sentieon DNAscope) 5. Variant calling - Structural variants (SV) (Tiddit & Manta) -6. SNV annotation & ranking (rohcall, vcfanno, ensembl VEP, GENMOD) -7. SV annotation & ranking (SVDB query, ensembl VEP, GENMOD) -8. Mitochondrial annotation +6. Copy number variant calling (GATK's GermlineCNVCaller) +7. SNV annotation & ranking (rohcall, vcfanno, ensembl VEP, GENMOD) +8. SV annotation & ranking (SVDB query, ensembl VEP, GENMOD) +9. Mitochondrial annotation > We have only listed the groups that require at least one input from the user. For example, the pipeline also runs SMNCopyNumberCaller, but it does not require any input other than the bam files passed by the pipeline. Hence, it is not mentioned in the list above. To know more about the tools used in the pipeline check the [README](../README.md). From ee105a8b60869ad588a9a212a5193b85c25c0881 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 13 Sep 2023 13:21:22 +0200 Subject: [PATCH 109/387] Update subworkflows/local/call_snv.nf [skip ci] Co-authored-by: Anders Jemt --- subworkflows/local/call_snv.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index d8cb9744..96320986 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -103,6 +103,6 @@ workflow CALL_SNV { genome_tabix = ch_genome_tabix // channel: [ val(meta), path(tbi) ] genome_vcf_tabix = ch_genome_vcf_tabix // channel: [ val(meta), path(vcf), path(tbi) ] mt_vcf = POSTPROCESS_MT_CALLS.out.vcf // channel: [ val(meta), path(vcf) ] - mt_tabix = POSTPROCESS_MT_CALLS.out.tbi // channel: [ val(meta), path(vcf) ] + mt_tabix = POSTPROCESS_MT_CALLS.out.tbi // channel: [ val(meta), path(tbi) ] versions = ch_versions // channel: [ path(versions.yml) ] } From 4eeab25d49c56b29dc79ed82de7247cfc2661ad0 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 13 Sep 2023 13:23:01 +0200 Subject: [PATCH 110/387] Update subworkflows/local/variant_calling/postprocess_MT_calls.nf [skip ci] Co-authored-by: Anders Jemt --- subworkflows/local/variant_calling/postprocess_MT_calls.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/variant_calling/postprocess_MT_calls.nf b/subworkflows/local/variant_calling/postprocess_MT_calls.nf index 81738085..2d10cfaa 100644 --- a/subworkflows/local/variant_calling/postprocess_MT_calls.nf +++ b/subworkflows/local/variant_calling/postprocess_MT_calls.nf @@ -25,7 +25,7 @@ workflow POSTPROCESS_MT_CALLS { main: ch_versions = Channel.empty() - // LIFTOVER VCF FROM REFERENCE MT TO SHIFTED MT + // LIFTOVER SHIFTED VCF TO REFERENCE MT POSITIONS PICARD_LIFTOVERVCF ( ch_mtshift_vcf, ch_genome_dictionary, From 9c1f1c0e0e4aea525e73e7e47fe4bfc7449ac1df Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 13 Sep 2023 14:48:52 +0200 Subject: [PATCH 111/387] comments --- assets/schema_input.json | 2 +- subworkflows/local/align.nf | 2 +- subworkflows/local/annotate_mt_snvs.nf | 10 +- .../local/call_structural_variants.nf | 2 - subworkflows/local/convert_mt_bam_to_fastq.nf | 36 ---- .../local/mitochondria/align_and_call_MT.nf | 96 ---------- .../local/mitochondria/merge_annotate_MT.nf | 176 ------------------ .../variant_calling/postprocess_MT_calls.nf | 4 +- 8 files changed, 9 insertions(+), 319 deletions(-) delete mode 100644 subworkflows/local/convert_mt_bam_to_fastq.nf delete mode 100644 subworkflows/local/mitochondria/align_and_call_MT.nf delete mode 100644 subworkflows/local/mitochondria/merge_annotate_MT.nf diff --git a/assets/schema_input.json b/assets/schema_input.json index 555f2c0a..191f1394 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,7 +10,7 @@ "sample": { "type": "string", "exists": true, - "meta": ["id"], + "meta": ["id", "sample"], "pattern": "^\\S+$", "errorMessage": "Sample name must be provided and cannot contain spaces" }, diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index e7314f2a..17a6252b 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -7,7 +7,7 @@ include { ALIGN_SENTIEON } from './alignment/align_sentieon' include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main' include { ALIGN_MT } from './alignment/align_MT' include { ALIGN_MT as ALIGN_MT_SHIFT } from './alignment/align_MT' -include { CONVERT_MT_BAM_TO_FASTQ } from './convert_mt_bam_to_fastq' +include { CONVERT_MT_BAM_TO_FASTQ } from './mitochondria/convert_mt_bam_to_fastq' workflow ALIGN { take: diff --git a/subworkflows/local/annotate_mt_snvs.nf b/subworkflows/local/annotate_mt_snvs.nf index 5a6b65b5..8f449e7b 100644 --- a/subworkflows/local/annotate_mt_snvs.nf +++ b/subworkflows/local/annotate_mt_snvs.nf @@ -36,12 +36,12 @@ workflow ANNOTATE_MT_SNVS { // Pick input for vep ch_mt_vcf - .combine(ANNOTATE_CADD.out.vcf.ifEmpty("null")) - .branch { it -> - merged: it[2].equals("null") + .join(ANNOTATE_CADD.out.vcf, remainder: true) // If CADD is not run then the third element in this channel will be `null` + .branch { it -> // If CADD is run, then "it" will be [[meta],selvar.vcf,cadd.vcf], else [[meta],selvar.vcf,null] + merged: it[2].equals(null) return [it[0], it[1]] - cadd: !(it[2].equals("null")) - return [it[2], it[3]] + cadd: !(it[2].equals(null)) + return [it[0], it[2]] } .set { ch_for_mix } ch_vep_in = ch_for_mix.merged.mix(ch_for_mix.cadd) diff --git a/subworkflows/local/call_structural_variants.nf b/subworkflows/local/call_structural_variants.nf index d0846e6e..28f4e8db 100644 --- a/subworkflows/local/call_structural_variants.nf +++ b/subworkflows/local/call_structural_variants.nf @@ -50,8 +50,6 @@ workflow CALL_STRUCTURAL_VARIANTS { CALL_SV_MT (ch_mt_bam_bai, ch_genome_fasta) -// CALL_SV_MT_SHIFT (ch_mtshift_bam_bai, ch_mtshift_fasta) - //merge if (params.skip_cnv_calling) { tiddit_vcf diff --git a/subworkflows/local/convert_mt_bam_to_fastq.nf b/subworkflows/local/convert_mt_bam_to_fastq.nf deleted file mode 100644 index ca19ea82..00000000 --- a/subworkflows/local/convert_mt_bam_to_fastq.nf +++ /dev/null @@ -1,36 +0,0 @@ -// -// Prepare bam files for MT allignment -// - -include { GATK4_PRINTREADS as GATK4_PRINTREADS_MT } from '../../modules/nf-core/gatk4/printreads/main' -include { GATK4_REVERTSAM as GATK4_REVERTSAM_MT } from '../../modules/nf-core/gatk4/revertsam/main' -include { GATK4_SAMTOFASTQ as GATK4_SAMTOFASTQ_MT } from '../../modules/nf-core/gatk4/samtofastq/main' - -workflow CONVERT_MT_BAM_TO_FASTQ { - take: - ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] - ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] - ch_genome_dict // channel: [mandatory] [ val(meta), path(dict) ] - - main: - ch_versions = Channel.empty() - - // Outputs bam containing only MT - GATK4_PRINTREADS_MT ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_genome_dict ) - - // Removes alignment information - GATK4_REVERTSAM_MT ( GATK4_PRINTREADS_MT.out.bam ) - - // Outputs fastq files - GATK4_SAMTOFASTQ_MT ( GATK4_REVERTSAM_MT.out.bam ) - - ch_versions = ch_versions.mix(GATK4_PRINTREADS_MT.out.versions.first()) - ch_versions = ch_versions.mix(GATK4_REVERTSAM_MT.out.versions.first()) - ch_versions = ch_versions.mix(GATK4_SAMTOFASTQ_MT.out.versions.first()) - - emit: - fastq = GATK4_SAMTOFASTQ_MT.out.fastq // channel: [ val(meta), [ path(fastq) ] ] - bam = GATK4_REVERTSAM_MT.out.bam // channel: [ val(meta), path(bam) ] - versions = ch_versions // channel: [ path(versions.yml) ] -} diff --git a/subworkflows/local/mitochondria/align_and_call_MT.nf b/subworkflows/local/mitochondria/align_and_call_MT.nf deleted file mode 100644 index d857b11a..00000000 --- a/subworkflows/local/mitochondria/align_and_call_MT.nf +++ /dev/null @@ -1,96 +0,0 @@ -// -// Align and call MT -// - -include { SENTIEON_BWAMEM as SENTIEON_BWAMEM_MT } from '../../../modules/nf-core/sentieon/bwamem/main' -include { BWAMEM2_MEM as BWAMEM2_MEM_MT } from '../../../modules/nf-core/bwamem2/mem/main' -include { GATK4_MERGEBAMALIGNMENT as GATK4_MERGEBAMALIGNMENT_MT } from '../../../modules/nf-core/gatk4/mergebamalignment/main' -include { PICARD_ADDORREPLACEREADGROUPS as PICARD_ADDORREPLACEREADGROUPS_MT } from '../../../modules/nf-core/picard/addorreplacereadgroups/main' -include { PICARD_MARKDUPLICATES as PICARD_MARKDUPLICATES_MT } from '../../../modules/nf-core/picard/markduplicates/main' -include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_MT } from '../../../modules/nf-core/samtools/index/main' -include { SAMTOOLS_SORT as SAMTOOLS_SORT_MT } from '../../../modules/nf-core/samtools/sort/main' -include { HAPLOCHECK as HAPLOCHECK_MT } from '../../../modules/nf-core/haplocheck/main' -include { GATK4_MUTECT2 as GATK4_MUTECT2_MT } from '../../../modules/nf-core/gatk4/mutect2/main' -include { GATK4_FILTERMUTECTCALLS as GATK4_FILTERMUTECTCALLS_MT } from '../../../modules/nf-core/gatk4/filtermutectcalls/main' -include { TABIX_TABIX as TABIX_TABIX_MT } from '../../../modules/nf-core/tabix/tabix/main' -include { MT_DELETION } from '../../../modules/local/mt_deletion_script' -include { EKLIPSE as EKLIPSE_MT } from '../../../modules/nf-core/eklipse/main' - -workflow ALIGN_AND_CALL_MT { - take: - ch_fastq // channel: [mandatory] [ val(meta), [ path(reads) ] ] - ch_ubam // channel: [mandatory] [ val(meta), path(bam) ] - ch_bwaindex // channel: [mandatory for sentieon] [ val(meta), path(index) ] - ch_bwamem2index // channel: [mandatory for bwamem2] [ val(meta), path(index) ] - ch_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_dict // channel: [mandatory] [ val(meta), path(dict) ] - ch_fai // channel: [mandatory] [ val(meta), path(fai) ] - ch_intervals // channel: [mandatory] [ path(interval_list) ] - - main: - ch_versions = Channel.empty() - - BWAMEM2_MEM_MT (ch_fastq, ch_bwamem2index, true) - - SENTIEON_BWAMEM_MT ( ch_fastq, ch_bwaindex, ch_fasta, ch_fai ) - - Channel.empty() - .mix(BWAMEM2_MEM_MT.out.bam, SENTIEON_BWAMEM_MT.out.bam_and_bai.map{ meta, bam, bai -> [meta, bam] }) - .join(ch_ubam, failOnMismatch:true, failOnDuplicate:true) - .set {ch_bam_ubam} - - GATK4_MERGEBAMALIGNMENT_MT (ch_bam_ubam, ch_fasta, ch_dict) - - PICARD_ADDORREPLACEREADGROUPS_MT (GATK4_MERGEBAMALIGNMENT_MT.out.bam) - - PICARD_MARKDUPLICATES_MT (PICARD_ADDORREPLACEREADGROUPS_MT.out.bam, ch_fasta, ch_fai) - - SAMTOOLS_SORT_MT (PICARD_MARKDUPLICATES_MT.out.bam) - - SAMTOOLS_INDEX_MT(SAMTOOLS_SORT_MT.out.bam) - ch_sort_index_bam = SAMTOOLS_SORT_MT.out.bam.join(SAMTOOLS_INDEX_MT.out.bai, failOnMismatch:true, failOnDuplicate:true) - ch_sort_index_bam_int_mt = ch_sort_index_bam.combine(ch_intervals) - - EKLIPSE_MT(ch_sort_index_bam,[]) - - MT_DELETION(ch_sort_index_bam, ch_fasta) - - GATK4_MUTECT2_MT (ch_sort_index_bam_int_mt, ch_fasta, ch_fai, ch_dict, [], [], [],[]) - - HAPLOCHECK_MT (GATK4_MUTECT2_MT.out.vcf) - - // Filter Mutect2 calls - ch_mutect_vcf = GATK4_MUTECT2_MT.out.vcf.join(GATK4_MUTECT2_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) - ch_mutect_out = ch_mutect_vcf.join(GATK4_MUTECT2_MT.out.stats, failOnMismatch:true, failOnDuplicate:true) - ch_to_filt = ch_mutect_out.map { - meta, vcf, tbi, stats -> - return [meta, vcf, tbi, stats, [], [], [], []] - } - - GATK4_FILTERMUTECTCALLS_MT (ch_to_filt, ch_fasta, ch_fai, ch_dict) - - ch_versions = ch_versions.mix(BWAMEM2_MEM_MT.out.versions.first()) - ch_versions = ch_versions.mix(GATK4_MERGEBAMALIGNMENT_MT.out.versions.first()) - ch_versions = ch_versions.mix(PICARD_ADDORREPLACEREADGROUPS_MT.out.versions.first()) - ch_versions = ch_versions.mix(PICARD_MARKDUPLICATES_MT.out.versions.first()) - ch_versions = ch_versions.mix(SAMTOOLS_SORT_MT.out.versions.first()) - ch_versions = ch_versions.mix(SAMTOOLS_INDEX_MT.out.versions.first()) - ch_versions = ch_versions.mix(EKLIPSE_MT.out.versions.first()) - ch_versions = ch_versions.mix(MT_DELETION.out.versions.first()) - ch_versions = ch_versions.mix(GATK4_MUTECT2_MT.out.versions.first()) - ch_versions = ch_versions.mix(HAPLOCHECK_MT.out.versions.first()) - ch_versions = ch_versions.mix(GATK4_FILTERMUTECTCALLS_MT.out.versions.first()) - - emit: - vcf = GATK4_FILTERMUTECTCALLS_MT.out.vcf // channel: [ val(meta), path(vcf) ] - tbi = GATK4_FILTERMUTECTCALLS_MT.out.tbi // channel: [ val(meta), path(tbi) ] - stats = GATK4_MUTECT2_MT.out.stats // channel: [ val(meta), path(stats) ] - filt_stats = GATK4_FILTERMUTECTCALLS_MT.out.stats // channel: [ val(meta), path(tsv) ] - eklipse_del = EKLIPSE_MT.out.deletions // channel: [ val(meta), path(csv) ] - eklipse_genes = EKLIPSE_MT.out.genes // channel: [ val(meta), path(csv) ] - eklipse_circos = EKLIPSE_MT.out.circos // channel: [ val(meta), path(png) ] - txt = HAPLOCHECK_MT.out.txt // channel: [ val(meta), path(txt) ] - html = HAPLOCHECK_MT.out.html // channel: [ val(meta), path(html) ] - mt_del_result = MT_DELETION.out.mt_del_result // channel: [ val(meta), path(txt) ] - versions = ch_versions // channel: [ path(versions.yml) ] -} diff --git a/subworkflows/local/mitochondria/merge_annotate_MT.nf b/subworkflows/local/mitochondria/merge_annotate_MT.nf deleted file mode 100644 index 43edd93b..00000000 --- a/subworkflows/local/mitochondria/merge_annotate_MT.nf +++ /dev/null @@ -1,176 +0,0 @@ -// -// Merge and annotate MT -// - -include { GATK4_MERGEVCFS as GATK4_MERGEVCFS_LIFT_UNLIFT_MT } from '../../../modules/nf-core/gatk4/mergevcfs/main' -include { GATK4_VARIANTFILTRATION as GATK4_VARIANTFILTRATION_MT } from '../../../modules/nf-core/gatk4/variantfiltration/main' -include { BCFTOOLS_NORM as SPLIT_MULTIALLELICS_MT } from '../../../modules/nf-core/bcftools/norm/main' -include { TABIX_TABIX as TABIX_TABIX_MT } from '../../../modules/nf-core/tabix/tabix/main' -include { BCFTOOLS_NORM as REMOVE_DUPLICATES_MT } from '../../../modules/nf-core/bcftools/norm/main' -include { TABIX_TABIX as TABIX_TABIX_MT2 } from '../../../modules/nf-core/tabix/tabix/main' -include { BCFTOOLS_MERGE as BCFTOOLS_MERGE_MT } from '../../../modules/nf-core/bcftools/merge/main' -include { TABIX_TABIX as TABIX_TABIX_MERGE } from '../../../modules/nf-core/tabix/tabix/main' -include { TABIX_TABIX as TABIX_TABIX_MT3 } from '../../../modules/nf-core/tabix/tabix/main' -include { ENSEMBLVEP as ENSEMBLVEP_MT } from '../../../modules/local/ensemblvep/main' -include { HAPLOGREP2_CLASSIFY as HAPLOGREP2_CLASSIFY_MT } from '../../../modules/nf-core/haplogrep2/classify/main' -include { VCFANNO as VCFANNO_MT } from '../../../modules/nf-core/vcfanno/main' -include { ANNOTATE_CADD } from '../annotation/annotate_cadd' -include { TABIX_BGZIPTABIX as ZIP_TABIX_HMTNOTE } from '../../../modules/nf-core/tabix/bgziptabix/main' -include { HMTNOTE_ANNOTATE } from '../../../modules/nf-core/hmtnote/annotate/main' - -workflow MERGE_ANNOTATE_MT { - take: - ch_vcf1 // channel: [mandatory] [ val(meta), path(vcf) ] - ch_vcf2 // channel: [mandatory] [ val(meta), path(vcf) ] - ch_cadd_header // channel: [mandatory] [ path(txt) ] - ch_cadd_resources // channel: [mandatory] [ path(annotation) ] - ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_genome_dict // channel: [mandatory] [ val(meta), path(dict) ] - ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] - ch_vcfanno_resources // channel: [mandatory] [ path(resources) ] - ch_vcfanno_toml // channel: [mandatory] [ path(toml) ] - val_vep_genome // string: [mandatory] GRCh37 or GRCh38 - val_vep_cache_version // string: [mandatory] 107 - ch_vep_cache // channel: [mandatory] [ path(cache) ] - ch_case_info // channel: [mandatory] [ val(case_info) ] - - main: - ch_versions = Channel.empty() - - ch_vcfs = ch_vcf1 - .join(ch_vcf2, remainder: true) - .map{ meta, vcf1, vcf2 -> - [meta, [vcf1, vcf2]] - } - GATK4_MERGEVCFS_LIFT_UNLIFT_MT( ch_vcfs, ch_genome_dict) - - // Filtering Variants - GATK4_MERGEVCFS_LIFT_UNLIFT_MT.out.vcf - .join(GATK4_MERGEVCFS_LIFT_UNLIFT_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) - .set { ch_filt_vcf } - GATK4_VARIANTFILTRATION_MT (ch_filt_vcf, ch_genome_fasta, ch_genome_fai, ch_genome_dict) - - // Spliting multiallelic calls - GATK4_VARIANTFILTRATION_MT.out.vcf - .join(GATK4_VARIANTFILTRATION_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) - .set { ch_in_split } - SPLIT_MULTIALLELICS_MT (ch_in_split, ch_genome_fasta) - TABIX_TABIX_MT(SPLIT_MULTIALLELICS_MT.out.vcf) - - // Removing duplicates and merging if there is more than one sample - SPLIT_MULTIALLELICS_MT.out.vcf - .join(TABIX_TABIX_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) - .set { ch_in_remdup } - REMOVE_DUPLICATES_MT(ch_in_remdup, ch_genome_fasta) - TABIX_TABIX_MT2(REMOVE_DUPLICATES_MT.out.vcf) - - REMOVE_DUPLICATES_MT.out.vcf - .collect{it[1]} - .ifEmpty([]) - .toList() - .set { file_list_vcf } - - TABIX_TABIX_MT2.out.tbi - .collect{it[1]} - .ifEmpty([]) - .toList() - .set { file_list_tbi } - - ch_case_info - .combine(file_list_vcf) - .combine(file_list_tbi) - .set { ch_rem_dup_vcf_tbi } - - ch_rem_dup_vcf_tbi.branch { - meta, vcf, tbi -> - single: vcf.size() == 1 - return [meta, vcf] - multiple: vcf.size() > 1 - return [meta, vcf, tbi] - }.set { ch_case_vcf } - - BCFTOOLS_MERGE_MT( ch_case_vcf.multiple, - ch_genome_fasta, - ch_genome_fai, - [] - ) - - BCFTOOLS_MERGE_MT.out.merged_variants - .mix(ch_case_vcf.single) - .set { ch_annotation_in } - - TABIX_TABIX_MERGE(ch_annotation_in) - - // Annotating with CADD - ANNOTATE_CADD ( - ch_annotation_in, - TABIX_TABIX_MERGE.out.tbi, - ch_cadd_header, - ch_cadd_resources - ) - - // Pick input for vep - ch_annotation_in - .combine(ANNOTATE_CADD.out.vcf.ifEmpty("null")) - .branch { it -> - merged: it[2].equals("null") - return [it[0], it[1]] - cadd: !(it[2].equals("null")) - return [it[2], it[3]] - } - .set { ch_for_mix } - ch_vep_in = ch_for_mix.merged.mix(ch_for_mix.cadd) - - // Annotating with ensembl Vep - ENSEMBLVEP_MT( - ch_vep_in, - ch_genome_fasta, - val_vep_genome, - "homo_sapiens", - val_vep_cache_version, - ch_vep_cache, - [] - ) - - // Running vcfanno - TABIX_TABIX_MT3(ENSEMBLVEP_MT.out.vcf_gz) - ENSEMBLVEP_MT.out.vcf_gz - .join(TABIX_TABIX_MT3.out.tbi, failOnMismatch:true, failOnDuplicate:true) - .map { meta, vcf, tbi -> return [meta, vcf, tbi, []]} - .set { ch_in_vcfanno } - - VCFANNO_MT(ch_in_vcfanno, ch_vcfanno_toml, [], ch_vcfanno_resources) - - // HMTNOTE ANNOTATE - HMTNOTE_ANNOTATE(VCFANNO_MT.out.vcf) - HMTNOTE_ANNOTATE.out.vcf.map{meta, vcf -> - return [meta, WorkflowRaredisease.replaceSpacesInInfoColumn(vcf, vcf.parent.toString(), vcf.baseName)] - } - .set { ch_hmtnote_reformatted } - ZIP_TABIX_HMTNOTE(ch_hmtnote_reformatted) - - // Prepare output - ch_vcf_out = ZIP_TABIX_HMTNOTE.out.gz_tbi.map{meta, vcf, tbi -> return [meta, vcf] } - ch_tbi_out = ZIP_TABIX_HMTNOTE.out.gz_tbi.map{meta, vcf, tbi -> return [meta, tbi] } - - // Running haplogrep2 - HAPLOGREP2_CLASSIFY_MT(ch_vep_in, "vcf.gz") - - ch_versions = ch_versions.mix(GATK4_MERGEVCFS_LIFT_UNLIFT_MT.out.versions.first()) - ch_versions = ch_versions.mix(GATK4_VARIANTFILTRATION_MT.out.versions.first()) - ch_versions = ch_versions.mix(SPLIT_MULTIALLELICS_MT.out.versions.first()) - ch_versions = ch_versions.mix(REMOVE_DUPLICATES_MT.out.versions.first()) - ch_versions = ch_versions.mix(BCFTOOLS_MERGE_MT.out.versions) - ch_versions = ch_versions.mix(ANNOTATE_CADD.out.versions) - ch_versions = ch_versions.mix(ENSEMBLVEP_MT.out.versions) - ch_versions = ch_versions.mix(VCFANNO_MT.out.versions) - ch_versions = ch_versions.mix(HMTNOTE_ANNOTATE.out.versions) - ch_versions = ch_versions.mix(HAPLOGREP2_CLASSIFY_MT.out.versions) - - emit: - haplog = HAPLOGREP2_CLASSIFY_MT.out.txt // channel: [ val(meta), path(txt) ] - vcf = ch_vcf_out // channel: [ val(meta), path(vcf) ] - tbi = ch_tbi_out // channel: [ val(meta), path(tbi) ] - report = ENSEMBLVEP_MT.out.report // channel: [ path(html) ] - versions = ch_versions // channel: [ path(versions.yml) ] -} diff --git a/subworkflows/local/variant_calling/postprocess_MT_calls.nf b/subworkflows/local/variant_calling/postprocess_MT_calls.nf index 81738085..cb14c9b7 100644 --- a/subworkflows/local/variant_calling/postprocess_MT_calls.nf +++ b/subworkflows/local/variant_calling/postprocess_MT_calls.nf @@ -1,5 +1,5 @@ // -// Merge and annotate MT +// Merge and normalize MT variants // include { GATK4_MERGEVCFS as GATK4_MERGEVCFS_LIFT_UNLIFT_MT } from '../../../modules/nf-core/gatk4/mergevcfs/main' @@ -25,7 +25,7 @@ workflow POSTPROCESS_MT_CALLS { main: ch_versions = Channel.empty() - // LIFTOVER VCF FROM REFERENCE MT TO SHIFTED MT + // LIFTOVER SHIFTED VCF TO REFERENCE MT POSITIONS PICARD_LIFTOVERVCF ( ch_mtshift_vcf, ch_genome_dictionary, From 1fe33557026ded3437da8a4f7fd92da6a9836a6c Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 13 Sep 2023 17:09:35 +0200 Subject: [PATCH 112/387] add sample to meta --- assets/schema_input.json | 2 +- subworkflows/local/alignment/align_bwamem2.nf | 2 +- subworkflows/local/alignment/align_sentieon.nf | 2 +- workflows/raredisease.nf | 8 +++----- 4 files changed, 6 insertions(+), 8 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index 555f2c0a..191f1394 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,7 +10,7 @@ "sample": { "type": "string", "exists": true, - "meta": ["id"], + "meta": ["id", "sample"], "pattern": "^\\S+$", "errorMessage": "Sample name must be provided and cannot contain spaces" }, diff --git a/subworkflows/local/alignment/align_bwamem2.nf b/subworkflows/local/alignment/align_bwamem2.nf index d35fd9de..75bd723e 100644 --- a/subworkflows/local/alignment/align_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwamem2.nf @@ -33,7 +33,7 @@ workflow ALIGN_BWAMEM2 { // Merge multiple lane samples and index BWAMEM2_MEM.out.bam .map{ meta, bam -> - new_id = meta.id.split('_')[0] + new_id = meta.sample new_meta = meta + [id:new_id, read_group:"\'@RG\\tID:" + new_id + "\\tPL:" + val_platform + "\\tSM:" + new_id + "\'"] [groupKey(new_meta, new_meta.num_lanes), bam] } diff --git a/subworkflows/local/alignment/align_sentieon.nf b/subworkflows/local/alignment/align_sentieon.nf index 044b0fb6..366f6cd9 100644 --- a/subworkflows/local/alignment/align_sentieon.nf +++ b/subworkflows/local/alignment/align_sentieon.nf @@ -22,7 +22,7 @@ workflow ALIGN_SENTIEON { SENTIEON_BWAMEM.out .bam_and_bai .map{ meta, bam, bai -> - new_id = meta.id.split('_')[0] + new_id = meta.sample new_meta = meta + [id:new_id, read_group:"\'@RG\\tID:" + new_id + "\\tPL:" + val_platform + "\\tSM:" + new_id + "\'"] [groupKey(new_meta, new_meta.num_lanes), bam, bai] } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 3499344e..e85813df 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -615,9 +615,7 @@ def makePed(samples) { outfile.text = ['#family_id', 'sample_id', 'father', 'mother', 'sex', 'phenotype'].join('\t') def samples_list = [] for(int i = 0; i Date: Wed, 13 Sep 2023 17:18:10 +0200 Subject: [PATCH 113/387] update changelog [skip ci] --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index bb277017..40644917 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -33,6 +33,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Updated modules from nf-core [#412](https://github.com/nf-core/raredisease/pull/412) - If present, remove duplicate entries in probands and upd_children in the meta. [#420](https://github.com/nf-core/raredisease/pull/420) - Fixes vep starting as many instances as the square of the number of scatters. [#405](https://github.com/nf-core/raredisease/pull/405) +- Removed logic where we added an arbitrary substring to keep file names unique after alignment, and then split those string to get at the original string before merging with a simple copy operation. ### `Updated` From 416f3d5d9f74ffe83d81f1daa6ad0406fad3df65 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 13 Sep 2023 17:20:18 +0200 Subject: [PATCH 114/387] Update CHANGELOG.md --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 40644917..aad514d1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -33,7 +33,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Updated modules from nf-core [#412](https://github.com/nf-core/raredisease/pull/412) - If present, remove duplicate entries in probands and upd_children in the meta. [#420](https://github.com/nf-core/raredisease/pull/420) - Fixes vep starting as many instances as the square of the number of scatters. [#405](https://github.com/nf-core/raredisease/pull/405) -- Removed logic where we added an arbitrary substring to keep file names unique after alignment, and then split those string to get at the original string before merging with a simple copy operation. +- Removed logic where we added an arbitrary substring to keep file names unique after alignment, and then split those string to get at the original string before merging with a simple copy operation. [#425](https://github.com/nf-core/raredisease/pull/425/files) ### `Updated` From f08fab2ae7cd117bd54d2bf7dfe557bfa21269f7 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 14 Sep 2023 17:03:45 +0200 Subject: [PATCH 115/387] Update CHANGELOG.md --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index aad514d1..786e2829 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -33,7 +33,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Updated modules from nf-core [#412](https://github.com/nf-core/raredisease/pull/412) - If present, remove duplicate entries in probands and upd_children in the meta. [#420](https://github.com/nf-core/raredisease/pull/420) - Fixes vep starting as many instances as the square of the number of scatters. [#405](https://github.com/nf-core/raredisease/pull/405) -- Removed logic where we added an arbitrary substring to keep file names unique after alignment, and then split those string to get at the original string before merging with a simple copy operation. [#425](https://github.com/nf-core/raredisease/pull/425/files) +- Replaced the logic where we added an arbitrary substring to keep file names unique after alignment which we then removed using a split operator, with a simple copy operation. [#425](https://github.com/nf-core/raredisease/pull/425/files) ### `Updated` From e9bdbfcd37d88a6e0ebccddee2f5a1382e39c849 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 15 Sep 2023 14:37:40 +0200 Subject: [PATCH 116/387] skip mt analysis --- conf/test.config | 1 + 1 file changed, 1 insertion(+) diff --git a/conf/test.config b/conf/test.config index 9a13845b..652849a6 100644 --- a/conf/test.config +++ b/conf/test.config @@ -25,6 +25,7 @@ params { // analysis params skip_cnv_calling = true + skip_mt_analysis = true // Input data input = 'https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/testdata/samplesheet_trio.csv' From 29c9c85ea9daf0b83fe6437c1ae9b23055d80eb1 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 15 Sep 2023 23:36:59 +0200 Subject: [PATCH 117/387] update test config --- conf/test.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/test.config b/conf/test.config index 10e89c43..c7593f76 100644 --- a/conf/test.config +++ b/conf/test.config @@ -24,8 +24,8 @@ params { mito_name = 'MT' // analysis params - skip_cnv_calling = true - skip_mt_analysis = true + skip_cnv_calling = true + skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI // Input data input = 'https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/testdata/samplesheet_trio.csv' From e072032a9138390712bdef4069125b5a55c6e0f0 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 20 Sep 2023 15:38:32 +0200 Subject: [PATCH 118/387] add metromap --- docs/images/raredisease_metromap.png | Bin 0 -> 266073 bytes docs/images/raredisease_metromap.svg | 1441 ++++++++++++++++++++++++++ 2 files changed, 1441 insertions(+) create mode 100644 docs/images/raredisease_metromap.png create mode 100644 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z<5`|a0XW>VCoM7`W}3wYu?ZPV8D{X5w3x-R`b+Vl|J0e#f6lo({70r~dK)WzgT_gE{y=v)Rm!@c*Fk{<&U2;Vbq}@&|k)s=WX2lmGu6gk6lj z?r}~I4vquAb|ZATx=sLhIU3};em?~KjAoJlxx?Xa7WAKc&1IVZx#!Hen?=iH&{M5{ PKVW8TWmI?G + + +sentieon-bwasentieon-dedupmarkduplicatesbwamem2alignment to mitochondria (bwamem2/bwa)markduplicatesmutect2mutect2markduplicatesalignment to shifted mitochondria (bwamem2/bwa)sentieon-dnascopesentieon-dnamodelapplydeepvariantglnexusbcftools - rohstrangerupdvcfannocaddvepgenmodcaddvephmtnotegenmodmanta + tiddit + cnvnatorvepsvdb-querygenmodmanta + tiddit + cnvnator + germlinecnvcallerexpansionhuntermultiqcpicardtools+mosdepthvcfannofastqfastqbambam vcf vcf vcf vcfeklipsedefault path traversed by the pipelineusers can configure the pipeline to traverse this path instead of the defaultusers have the option to not run these tools From 6e12271c30833d6d7c48b56dea19ccc244416dfe Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 20 Sep 2023 15:56:58 +0200 Subject: [PATCH 119/387] fixes rendering issue --- docs/images/raredisease_metromap.svg | 11 +++++++++-- 1 file changed, 9 insertions(+), 2 deletions(-) diff --git a/docs/images/raredisease_metromap.svg b/docs/images/raredisease_metromap.svg index 537e3d98..dabf621f 100644 --- a/docs/images/raredisease_metromap.svg +++ b/docs/images/raredisease_metromap.svg @@ -9,7 +9,7 @@ id="svg1" xml:space="preserve" inkscape:version="1.3 (0e150ed, 2023-07-21)" - sodipodi:docname="raredisease_workflow.svg" + sodipodi:docname="drawing.svg" inkscape:export-filename="raredisease_workflow.png" inkscape:export-xdpi="96" inkscape:export-ydpi="96" @@ -1438,4 +1438,11 @@ style="font-size:4.93889px;stroke-width:0.264583" x="402.06009" y="307.42535" - id="tspan163">users have the option to not run these tools + id="tspan163">users have the option to not run these tools From fb1f95bdda6a610374cda07d36ef93d3cc857e66 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj 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germlinecnvcallerexpansionhunterexpansionhuntermultiqcmultiqcpicardtools+mosdepthvcfannovcfannoreferenceseklipseeklipseusers can configure the pipeline to traverse this users can configure the pipeline to path instead of the defaulttraverse this path instead of the default + ry="3.3517513" /> From a0d58e581a2ae1d17c99e64ef23a96acd3eb3eb2 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 26 Sep 2023 08:08:54 +0000 Subject: [PATCH 121/387] Template update for nf-core/tools version 2.10 --- .devcontainer/devcontainer.json | 1 + .github/CONTRIBUTING.md | 4 +- .github/workflows/linting.yml | 2 +- .github/workflows/release-announcments.yml | 68 +++++++++ CHANGELOG.md | 2 +- CITATIONS.md | 2 +- CODE_OF_CONDUCT.md | 133 ++++++++++++++---- README.md | 21 +-- assets/multiqc_config.yml | 4 +- conf/modules.config | 9 ++ docs/output.md | 5 +- docs/usage.md | 16 ++- lib/NfcoreTemplate.groovy | 16 +++ lib/WorkflowRaredisease.groovy | 2 +- main.nf | 3 + modules.json | 6 +- .../custom/dumpsoftwareversions/main.nf | 2 +- modules/nf-core/fastqc/main.nf | 8 +- modules/nf-core/multiqc/main.nf | 2 +- nextflow.config | 9 +- nextflow_schema.json | 15 -- workflows/raredisease.nf | 1 + 22 files changed, 253 insertions(+), 78 deletions(-) create mode 100644 .github/workflows/release-announcments.yml diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index ea27a584..4ecfbfe3 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -2,6 +2,7 @@ "name": "nfcore", "image": "nfcore/gitpod:latest", "remoteUser": "gitpod", + "runArgs": ["--privileged"], // Configure tool-specific properties. "customizations": { diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 76760152..dc147015 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,7 +9,9 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -> If you need help using or modifying nf-core/raredisease then the best place to ask is on the nf-core Slack [#raredisease](https://nfcore.slack.com/channels/raredisease) channel ([join our Slack here](https://nf-co.re/join/slack)). +:::info +If you need help using or modifying nf-core/raredisease then the best place to ask is on the nf-core Slack [#raredisease](https://nfcore.slack.com/channels/raredisease) channel ([join our Slack here](https://nf-co.re/join/slack)). +::: ## Contribution workflow diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 888cb4bc..b8bdd214 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -78,7 +78,7 @@ jobs: - uses: actions/setup-python@v4 with: - python-version: "3.8" + python-version: "3.11" architecture: "x64" - name: Install dependencies diff --git a/.github/workflows/release-announcments.yml b/.github/workflows/release-announcments.yml new file mode 100644 index 00000000..6ad33927 --- /dev/null +++ b/.github/workflows/release-announcments.yml @@ -0,0 +1,68 @@ +name: release-announcements +# Automatic release toot and tweet anouncements +on: + release: + types: [published] + workflow_dispatch: + +jobs: + toot: + runs-on: ubuntu-latest + steps: + - uses: rzr/fediverse-action@master + with: + access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} + host: "mstdn.science" # custom host if not "mastodon.social" (default) + # GitHub event payload + # https://docs.github.com/en/developers/webhooks-and-events/webhooks/webhook-events-and-payloads#release + message: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + + send-tweet: + runs-on: ubuntu-latest + + steps: + - uses: actions/setup-python@v4 + with: + python-version: "3.10" + - name: Install dependencies + run: pip install tweepy==4.14.0 + - name: Send tweet + shell: python + run: | + import os + import tweepy + + client = tweepy.Client( + access_token=os.getenv("TWITTER_ACCESS_TOKEN"), + access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"), + consumer_key=os.getenv("TWITTER_CONSUMER_KEY"), + consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"), + ) + tweet = os.getenv("TWEET") + client.create_tweet(text=tweet) + env: + TWEET: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }} + TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }} + TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }} + TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }} + + bsky-post: + runs-on: ubuntu-latest + steps: + - uses: zentered/bluesky-post-action@v0.0.2 + with: + post: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + env: + BSKY_IDENTIFIER: ${{ secrets.BSKY_IDENTIFIER }} + BSKY_PASSWORD: ${{ secrets.BSKY_PASSWORD }} + # diff --git a/CHANGELOG.md b/CHANGELOG.md index c2ea5e17..a1aaaaa1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.1.0dev - [date] +## v1.2.0dev - [date] Initial release of nf-core/raredisease, created with the [nf-core](https://nf-co.re/) template. diff --git a/CITATIONS.md b/CITATIONS.md index 5521c755..0c0d7bd5 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -12,7 +12,7 @@ - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) - > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. Available online https://www.bioinformatics.babraham.ac.uk/projects/fastqc/. + > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md index f4fd052f..c089ec78 100644 --- a/CODE_OF_CONDUCT.md +++ b/CODE_OF_CONDUCT.md @@ -1,18 +1,20 @@ -# Code of Conduct at nf-core (v1.0) +# Code of Conduct at nf-core (v1.4) ## Our Pledge -In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: +In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: - Age +- Ability - Body size +- Caste - Familial status - Gender identity and expression - Geographical location - Level of experience - Nationality and national origins - Native language -- Physical and neurological ability +- Neurodiversity - Race or ethnicity - Religion - Sexual identity and orientation @@ -22,80 +24,133 @@ Please note that the list above is alphabetised and is therefore not ranked in a ## Preamble -> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply. +:::note +This Code of Conduct (CoC) has been drafted by Renuka Kudva, Cris Tuñí, and Michael Heuer, with input from the nf-core Core Team and Susanna Marquez from the nf-core community. "We", in this document, refers to the Safety Officers and members of the nf-core Core Team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will be amended periodically to keep it up-to-date. In case of any dispute, the most current version will apply. +::: -An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva. +An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). + +Our Safety Officers are Saba Nafees, Cris Tuñí, and Michael Heuer. nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. -We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc. +We have therefore adopted this CoC, which we require all members of our community and attendees of nf-core events to adhere to in all our workspaces at all times. Workspaces include, but are not limited to, Slack, meetings on Zoom, gather.town, YouTube live etc. -Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. +Our CoC will be strictly enforced and the nf-core team reserves the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. -We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. +We ask all members of our community to help maintain supportive and productive workspaces and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. -Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re +Questions, concerns, or ideas on what we can include? Contact members of the Safety Team on Slack or email safety [at] nf-co [dot] re. ## Our Responsibilities -The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. +Members of the Safety Team (the Safety Officers) are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. -The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. +The Safety Team, in consultation with the nf-core core team, have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this CoC, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. -Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC. +Members of the core team or the Safety Team who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and will be subject to the same actions as others in violation of the CoC. -## When are where does this Code of Conduct apply? +## When and where does this Code of Conduct apply? -Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference: +Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events, such as hackathons, workshops, bytesize, and collaborative workspaces on gather.town. These guidelines include, but are not limited to, the following (listed alphabetically and therefore in no order of preference): - Communicating with an official project email address. - Communicating with community members within the nf-core Slack channel. - Participating in hackathons organised by nf-core (both online and in-person events). -- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence. -- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc. +- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence, and on the nf-core gather.town workspace. +- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, gather.town, Jitsi, YouTube live etc. - Representing nf-core on social media. This includes both official and personal accounts. ## nf-core cares 😊 -nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order): +nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include, but are not limited to, the following (listed in alphabetical order): - Ask for consent before sharing another community member’s personal information (including photographs) on social media. - Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. -- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) +- Celebrate your accomplishments! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) - Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) - Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) - Focus on what is best for the team and the community. (When in doubt, ask) -- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn. +- Accept feedback, yet be unafraid to question, deliberate, and learn. - Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) -- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**) +- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communication to be kind.**) - Take breaks when you feel like you need them. -- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.) +- Use welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack) ## nf-core frowns on 😕 -The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces. +The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this CoC. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces: - Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. - “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. - Spamming or trolling of individuals on social media. -- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention. -- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience. +- Use of sexual or discriminatory imagery, comments, jokes, or unwelcome sexual attention. +- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion, or work experience. ### Online Trolling -The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately. +The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the risk of online trolling. This is unacceptable — reports of such behaviour will be taken very seriously and perpetrators will be excluded from activities immediately. -All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls. +All community members are **required** to ask members of the group they are working with for explicit consent prior to taking screenshots of individuals during video calls. -## Procedures for Reporting CoC violations +## Procedures for reporting CoC violations If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. -You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s). +You can reach out to members of the Safety Team (Saba Nafees, Cris Tuñí, and Michael Heuer) on Slack. Alternatively, contact a member of the nf-core core team [nf-core core team](https://nf-co.re/about), and they will forward your concerns to the Safety Team. + +Issues directly concerning members of the Core Team or the Safety Team will be dealt with by other members of the core team and the safety manager — possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson and details will be shared in due course. + +All reports will be handled with the utmost discretion and confidentiality. + +You can also report any CoC violations to safety [at] nf-co [dot] re. In your email report, please do your best to include: + +- Your contact information. +- Identifying information (e.g. names, nicknames, pseudonyms) of the participant who has violated the Code of Conduct. +- The behaviour that was in violation and the circumstances surrounding the incident. +- The approximate time of the behaviour (if different than the time the report was made). +- Other people involved in the incident, if applicable. +- If you believe the incident is ongoing. +- If there is a publicly available record (e.g. mailing list record, a screenshot). +- Any additional information. + +After you file a report, one or more members of our Safety Team will contact you to follow up on your report. + +## Who will read and handle reports + +All reports will be read and handled by the members of the Safety Team at nf-core. + +If members of the Safety Team are deemed to have a conflict of interest with a report, they will be required to recuse themselves as per our Code of Conduct and will not have access to any follow-ups. + +To keep this first report confidential from any of the Safety Team members, please submit your first report by direct messaging on Slack/direct email to any of the nf-core members you are comfortable disclosing the information to, and be explicit about which member(s) you do not consent to sharing the information with. + +## Reviewing reports + +After receiving the report, members of the Safety Team will review the incident report to determine whether immediate action is required, for example, whether there is immediate threat to participants’ safety. + +The Safety Team, in consultation with members of the nf-core core team, will assess the information to determine whether the report constitutes a Code of Conduct violation, for them to decide on a course of action. + +In the case of insufficient information, one or more members of the Safety Team may contact the reporter, the reportee, or any other attendees to obtain more information. -Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course. +Once additional information is gathered, the Safety Team will collectively review and decide on the best course of action to take, if any. The Safety Team reserves the right to not act on a report. -All reports will be handled with utmost discretion and confidentially. +## Confidentiality + +All reports, and any additional information included, are only shared with the team of safety officers (and possibly members of the core team, in case the safety officer is in violation of the CoC). We will respect confidentiality requests for the purpose of protecting victims of abuse. + +We will not name harassment victims, beyond discussions between the safety officer and members of the nf-core team, without the explicit consent of the individuals involved. + +## Enforcement + +Actions taken by the nf-core’s Safety Team may include, but are not limited to: + +- Asking anyone to stop a behaviour. +- Asking anyone to leave the event and online spaces either temporarily, for the remainder of the event, or permanently. +- Removing access to the gather.town and Slack, either temporarily or permanently. +- Communicating to all participants to reinforce our expectations for conduct and remind what is unacceptable behaviour; this may be public for practical reasons. +- Communicating to all participants that an incident has taken place and how we will act or have acted — this may be for the purpose of letting event participants know we are aware of and dealing with the incident. +- Banning anyone from participating in nf-core-managed spaces, future events, and activities, either temporarily or permanently. +- No action. ## Attribution and Acknowledgements @@ -106,6 +161,22 @@ All reports will be handled with utmost discretion and confidentially. ## Changelog -### v1.0 - March 12th, 2021 +### v1.4 - February 8th, 2022 + +- Included a new member of the Safety Team. Corrected a typographical error in the text. + +### v1.3 - December 10th, 2021 + +- Added a statement that the CoC applies to nf-core gather.town workspaces. Corrected typographical errors in the text. + +### v1.2 - November 12th, 2021 + +- Removed information specific to reporting CoC violations at the Hackathon in October 2021. + +### v1.1 - October 14th, 2021 + +- Updated with names of new Safety Officers and specific information for the hackathon in October 2021. + +### v1.0 - March 15th, 2021 - Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/README.md b/README.md index 89a39c08..bad14cdf 100644 --- a/README.md +++ b/README.md @@ -1,6 +1,7 @@ # ![nf-core/raredisease](docs/images/nf-core-raredisease_logo_light.png#gh-light-mode-only) ![nf-core/raredisease](docs/images/nf-core-raredisease_logo_dark.png#gh-dark-mode-only) -[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/raredisease/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![GitHub Actions CI Status](https://github.com/nf-core/raredisease/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/raredisease/actions?query=workflow%3A%22nf-core+CI%22) +[![GitHub Actions Linting Status](https://github.com/nf-core/raredisease/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/raredisease/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/raredisease/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) @@ -29,10 +30,11 @@ ## Usage -> **Note** -> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how -> to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) -> with `-profile test` before running the workflow on actual data. +:::note +If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how +to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) +with `-profile test` before running the workflow on actual data. +::: -

- -

- Note that it is possible to include/exclude certain tools or steps. ## Usage From 52719d1c2d94f487b44e4680f78dc3f5f9a142e3 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 3 Oct 2023 14:26:44 +0200 Subject: [PATCH 125/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 745172b9..59f1b418 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -9,6 +9,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Use `nf-validation` plugin for parameter and samplesheet validation [#386](https://github.com/nf-core/raredisease/pull/386) - A new parameter to skip filtering based on vep results [#416](https://github.com/nf-core/raredisease/pull/416) +- A `metromap` representating the core parts of the pipeline [#428](https://github.com/nf-core/raredisease/pull/428) ### `Changed` From 0f9b00367c2ab01014c05046377689f6694d160c Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 3 Oct 2023 14:49:03 +0200 Subject: [PATCH 126/387] add images --- README.md | 2 +- docs/images/nf-core-raredisease_logo_dark.png | Bin 75872 -> 32132 bytes docs/images/nf-core-raredisease_logo_dark.svg | 319 +++ .../images/nf-core-raredisease_logo_light.png | Bin 75763 -> 28311 bytes .../images/nf-core-raredisease_logo_light.svg | 327 ++++ docs/images/raredisease_metromap_dark.pdf | Bin 0 -> 38296 bytes docs/images/raredisease_metromap_dark.png | Bin 0 -> 263779 bytes docs/images/raredisease_metromap_dark.svg | 1705 +++++++++++++++++ ...map.png => raredisease_metromap_light.png} | Bin ...map.svg => raredisease_metromap_light.svg} | 0 10 files changed, 2352 insertions(+), 1 deletion(-) mode change 100755 => 100644 docs/images/nf-core-raredisease_logo_dark.png create mode 100644 docs/images/nf-core-raredisease_logo_dark.svg mode change 100755 => 100644 docs/images/nf-core-raredisease_logo_light.png create mode 100644 docs/images/nf-core-raredisease_logo_light.svg create mode 100644 docs/images/raredisease_metromap_dark.pdf create mode 100644 docs/images/raredisease_metromap_dark.png create mode 100644 docs/images/raredisease_metromap_dark.svg rename docs/images/{raredisease_metromap.png => raredisease_metromap_light.png} (100%) rename docs/images/{raredisease_metromap.svg => raredisease_metromap_light.svg} (100%) diff --git a/README.md b/README.md index 2cdf97b7..7801900f 100644 --- a/README.md +++ b/README.md @@ -32,7 +32,7 @@ On release, automated continuous integration tests run the pipeline on a full-si ## Pipeline summary

- +

**1. 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zTFL7PJ>>jbUsSi!HRd@HmF$`IPkPFAbZ(?reM$I$@lVM2e{uEBGwXkS$nQ?@#gXfq z95!2)x7P!7ffFK9`eHK{SFq@|P`%s*+KU(Ee!R=!q){L}1if2zm;3wflOg~8Yrbqw zI!4o2$>Jdk3Y%F_xOhzTPOo5h3{zLBf^*NjLzx59 zgetIKw|9?w|DEpAX;Qua%QL*WjQ^T6=qy@3I3ywBiwwyix4DA?eV&6c3>8(b*IUbv z{}<5W5@%m!!E~81=T=5~^QhAcalmN%7xY|NhC4s{Hwc|U{goSNg2b>>e3Y{q_)$3k Naqoe8m6}c1{{z*k&;bAd From cf4872575bb6d44d6cccbf98a5f72537bad80f5b Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 3 Oct 2023 15:01:41 +0200 Subject: [PATCH 128/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 59f1b418..2def1d5b 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,6 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Use `nf-validation` plugin for parameter and samplesheet validation [#386](https://github.com/nf-core/raredisease/pull/386) - A new parameter to skip filtering based on vep results [#416](https://github.com/nf-core/raredisease/pull/416) - A `metromap` representating the core parts of the pipeline [#428](https://github.com/nf-core/raredisease/pull/428) +- Metromap and logos for light and dark theme [#432](https://github.com/nf-core/raredisease/pull/432) ### `Changed` From d2a3d5f2fc57620cb01a0388b55c37f2730d30d3 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 3 Oct 2023 15:08:04 +0200 Subject: [PATCH 129/387] update lintconfig --- .nf-core.yml | 2 ++ 1 file changed, 2 insertions(+) diff --git a/.nf-core.yml b/.nf-core.yml index 93aadae1..629a06e7 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -3,6 +3,8 @@ lint: - conf/modules.config files_unchanged: - .github/PULL_REQUEST_TEMPLATE.md + - docs/images/nf-core-raredisease_logo_dark.png + - docs/images/nf-core-raredisease_logo_light.png - assets/multiqc_config.yaml - .github/workflows/linting.yml - pyproject.toml From 20ff43147f2ba0840ec1a41b9a8166ddc3570e3f Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 16 Oct 2023 15:59:29 +0200 Subject: [PATCH 130/387] add modules --- modules/nf-core/cnvnator/cnvnator/main.nf | 63 +++++++++++++++ modules/nf-core/cnvnator/cnvnator/meta.yml | 77 +++++++++++++++++++ modules/nf-core/cnvnator/convert2vcf/main.nf | 46 +++++++++++ modules/nf-core/cnvnator/convert2vcf/meta.yml | 42 ++++++++++ 4 files changed, 228 insertions(+) create mode 100644 modules/nf-core/cnvnator/cnvnator/main.nf create mode 100644 modules/nf-core/cnvnator/cnvnator/meta.yml create mode 100644 modules/nf-core/cnvnator/convert2vcf/main.nf create mode 100644 modules/nf-core/cnvnator/convert2vcf/meta.yml diff --git a/modules/nf-core/cnvnator/cnvnator/main.nf b/modules/nf-core/cnvnator/cnvnator/main.nf new file mode 100644 index 00000000..6eb8a4f0 --- /dev/null +++ b/modules/nf-core/cnvnator/cnvnator/main.nf @@ -0,0 +1,63 @@ +process CNVNATOR_CNVNATOR { + tag "$meta.id" + label 'process_single' + + conda "bioconda::cnvnator=0.4.1" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/cnvnator:0.4.1--py310h2dce045_7': + 'biocontainers/cnvnator:0.4.1--py310h2dce045_7' }" + + input: + tuple val(meta), path(bam), path(bai) + tuple val(meta2), path(root) + tuple val(meta3), path(fasta) + tuple val(meta4), path(fai) + + output: + tuple val(output_meta), path("${prefix}.root"), emit: root + tuple val(output_meta), path("${prefix}.tab") , emit: tab, optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def input_cmd = bam ? "-tree ${bam}" : '' + output_meta = bam ? meta : meta2 + prefix = task.ext.prefix ?: bam ? "${meta.id}" : "${meta2.id}" + if (fasta) { + reference = fasta.isDirectory() ? "-d ${fasta}" : "-fasta ${fasta}" + } else { + reference = '' + } + calls_cmd = args.contains("-call") ? "> ${prefix}.tab" : '' + """ + cnvnator \\ + -root ${prefix}.root \\ + $args \\ + $reference \\ + $input_cmd \\ + $calls_cmd + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + CNVnator: \$(echo \$(cnvnator 2>&1 | sed -n '3p' | sed 's/CNVnator v//')) + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + prefix = task.ext.prefix ?: bam ? "${meta.id}" : "${meta2.id}" + output_meta = bam ? meta : meta2 + def calls_cmd = args.contains("-call") ? "touch ${prefix}.tab" : '' + """ + touch ${prefix}.root + $calls_cmd + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + CNVnator: \$(echo \$(cnvnator 2>&1 | sed -n '3p' | sed 's/CNVnator v//')) + END_VERSIONS + """ +} diff --git a/modules/nf-core/cnvnator/cnvnator/meta.yml b/modules/nf-core/cnvnator/cnvnator/meta.yml new file mode 100644 index 00000000..ea4f09be --- /dev/null +++ b/modules/nf-core/cnvnator/cnvnator/meta.yml @@ -0,0 +1,77 @@ +name: "cnvnator_cnvnator" +description: CNVnator is a command line tool for CNV/CNA analysis from depth-of-coverage by mapped reads. +keywords: + - cnvnator + - cnv + - cna +tools: + - "cnvnator": + description: "Tool for calling copy number variations." + homepage: "https://github.com/abyzovlab/CNVnator" + documentation: "https://github.com/abyzovlab/CNVnator/blob/master/README.md" + tool_dev_url: "https://github.com/abyzovlab/CNVnator" + licence: "['MIT']" + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - meta2: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - meta3: + type: map + description: | + Groovy Map containing reference information + e.g. `[ id:'test' ]` + - meta4: + type: map + description: | + Groovy Map containing reference information + e.g. `[ id:'test' ]` + - bam: + type: file + description: Sorted BAM/CRAM/SAM file + pattern: "*.bam" + - bai: + type: file + description: Sorted BAM/CRAM/SAM file + pattern: "*.bam" + - root: + type: file + description: ROOT file + pattern: "*.root" + - fasta: + type: file + description: Path to a directory containing fasta files or a fasta file + pattern: "*.fa" + - fai: + type: file + description: Path to a fasta file index + pattern: "*.fai" + +output: + - output_meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - root: + type: file + description: A ROOT file + pattern: "*.root" + - tab: + type: file + description: A tab file containing cnvnator calls + pattern: "*.tab" + +authors: + - "@ramprasadn" diff --git a/modules/nf-core/cnvnator/convert2vcf/main.nf b/modules/nf-core/cnvnator/convert2vcf/main.nf new file mode 100644 index 00000000..1ca05ab0 --- /dev/null +++ b/modules/nf-core/cnvnator/convert2vcf/main.nf @@ -0,0 +1,46 @@ +process CNVNATOR_CONVERT2VCF { + tag "$meta.id" + label 'process_single' + + conda "bioconda::cnvnator=0.4.1" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/cnvnator:0.4.1--py310h2dce045_7': + 'biocontainers/cnvnator:0.4.1--py310h2dce045_7' }" + + input: + tuple val(meta), path(calls) + + output: + tuple val(meta), path("*.vcf"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + cnvnator2VCF.pl \\ + ${calls} \\ + $args \\ + > ${prefix}.vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + CNVnator : \$(echo \$(cnvnator 2>&1 | sed -n '3p' | sed 's/CNVnator v//')) + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + CNVnator : \$(echo \$(cnvnator 2>&1 | sed -n '3p' | sed 's/CNVnator v//')) + END_VERSIONS + """ +} diff --git a/modules/nf-core/cnvnator/convert2vcf/meta.yml b/modules/nf-core/cnvnator/convert2vcf/meta.yml new file mode 100644 index 00000000..5df7bc65 --- /dev/null +++ b/modules/nf-core/cnvnator/convert2vcf/meta.yml @@ -0,0 +1,42 @@ +name: "cnvnator_convert2vcf" +description: convert2vcf.pl is command line tool to convert CNVnator calls to vcf format. +keywords: + - cnvnator + - cnv + - cna +tools: + - "cnvnator": + description: "Tool for calling copy number variations." + homepage: "https://github.com/abyzovlab/CNVnator" + documentation: "https://github.com/abyzovlab/CNVnator/blob/master/README.md" + tool_dev_url: "https://github.com/abyzovlab/CNVnator" + licence: "['MIT']" + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - calls: + type: file + description: A tab file containing CNVnator calls + pattern: "*.tab" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: CNVnator calls in vcf format + pattern: "*.vcf" + +authors: + - "@ramprasadn" From 83137a8b13c48cc39ff441fb914379f466395cd3 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 16 Oct 2023 15:59:50 +0200 Subject: [PATCH 131/387] modules.json --- modules.json | 10 ++++++++++ 1 file changed, 10 insertions(+) diff --git a/modules.json b/modules.json index 6416e6a1..db78a291 100644 --- a/modules.json +++ b/modules.json @@ -80,6 +80,16 @@ "git_sha": "aad210ba51500be029740d088b4b4827f6f41509", "installed_by": ["modules"] }, + "cnvnator/cnvnator": { + "branch": "master", + "git_sha": "e0fcafde1e74156695fc27e90a6ad6325065e6c5", + "installed_by": ["modules"] + }, + "cnvnator/convert2vcf": { + "branch": "master", + "git_sha": "e0fcafde1e74156695fc27e90a6ad6325065e6c5", + "installed_by": ["modules"] + }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "05c280924b6c768d484c7c443dad5e605c4ff4b4", From 01223da5d499161d02768e0093cd34eb2a3ee05e Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 16 Oct 2023 18:04:34 +0200 Subject: [PATCH 132/387] update subworkflows --- README.md | 1 + conf/modules/call_sv_cnvnator.config | 44 +++++++ conf/modules/call_sv_germlinecnvcaller.config | 2 +- conf/test.config | 2 +- conf/test_one_sample.config | 2 +- conf/test_sentieon.config | 2 +- docs/output.md | 112 ++++++++++-------- nextflow.config | 3 +- nextflow_schema.json | 4 +- .../local/call_structural_variants.nf | 10 +- .../local/variant_calling/call_sv_cnvnator.nf | 51 ++++++++ workflows/raredisease.nf | 8 +- 12 files changed, 177 insertions(+), 64 deletions(-) create mode 100644 conf/modules/call_sv_cnvnator.config create mode 100644 subworkflows/local/variant_calling/call_sv_cnvnator.nf diff --git a/README.md b/README.md index 7801900f..e9ebcbea 100644 --- a/README.md +++ b/README.md @@ -60,6 +60,7 @@ On release, automated continuous integration tests run the pipeline on a full-si - [Manta](https://github.com/Illumina/manta) - [TIDDIT's sv](https://github.com/SciLifeLab/TIDDIT) - Copy number variant calling: + - [CNVnator](https://github.com/abyzovlab/CNVnator) - [GATK GermlineCNVCaller](https://github.com/broadinstitute/gatk) **5. Annotation - SNV:** diff --git a/conf/modules/call_sv_cnvnator.config b/conf/modules/call_sv_cnvnator.config new file mode 100644 index 00000000..186bdc09 --- /dev/null +++ b/conf/modules/call_sv_cnvnator.config @@ -0,0 +1,44 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. + ext.when = Conditional clause +---------------------------------------------------------------------------------------- +*/ + +// +// cnvnator calling options +// + +process { + + withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_CNVNATOR:CNVNATOR_HIST" { + tag = { "$meta2.id" } + ext.args = '-his 1000' + } + + withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_CNVNATOR:CNVNATOR_STAT" { + tag = { "$meta2.id" } + ext.args = '-stat 1000' + } + + withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_CNVNATOR:CNVNATOR_PARTITION" { + tag = { "$meta2.id" } + ext.args = '-partition 1000' + } + + withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_CNVNATOR:CNVNATOR_CALL" { + tag = { "$meta2.id" } + ext.args = '-call 1000' + } + + withName: '.*CALL_STRUCTURAL_VARIANTS:CALL_SV_CNVNATOR:SVDB_MERGE_CNVNATOR' { + ext.args = '--notag --pass_only' + ext.prefix = { "${meta.id}_cnvnator" } + } +} diff --git a/conf/modules/call_sv_germlinecnvcaller.config b/conf/modules/call_sv_germlinecnvcaller.config index bf37b9d9..98f14d37 100644 --- a/conf/modules/call_sv_germlinecnvcaller.config +++ b/conf/modules/call_sv_germlinecnvcaller.config @@ -21,7 +21,7 @@ process { publishDir = [ enabled: false ] - ext.when = !params.skip_cnv_calling + ext.when = !params.skip_germlinecnvcaller } withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:GATK4_COLLECTREADCOUNTS" { diff --git a/conf/test.config b/conf/test.config index c7593f76..5fbde3f4 100644 --- a/conf/test.config +++ b/conf/test.config @@ -24,7 +24,7 @@ params { mito_name = 'MT' // analysis params - skip_cnv_calling = true + skip_germlinecnvcaller = true skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI // Input data diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index 51524fe7..dba0420b 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -24,7 +24,7 @@ params { mito_name = 'MT' // analysis params - skip_cnv_calling = true + skip_germlinecnvcaller = true skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI // Input data diff --git a/conf/test_sentieon.config b/conf/test_sentieon.config index f729c35e..64618d12 100644 --- a/conf/test_sentieon.config +++ b/conf/test_sentieon.config @@ -24,7 +24,7 @@ params { mito_name = 'MT' // analysis params - skip_cnv_calling = true + skip_germlinecnvcaller = true // Input data input = 'https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/testdata/samplesheet_trio.csv' diff --git a/docs/output.md b/docs/output.md index ef1c02a9..9509ef0a 100644 --- a/docs/output.md +++ b/docs/output.md @@ -10,57 +10,61 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -- [Alignment](#alignment) - - [Mapping](#mapping) - - [Bwa-mem2](#bwa-mem2) - - [Sentieon bwa mem](#sentieon-bwa-mem) - - [Duplicate marking](#duplicate-marking) - - [Picard's MarkDuplicates](#picard-s-markduplicates) - - [Sentieon dedup](#sentieon-dedup) -- [Quality control and reporting](#quality-control-and-reporting) - - [Quality control](#quality-control) - - [FastQC](#fastqc) - - [Mosdepth](#mosdepth) - - [Picard tools](#picard-tools) - - [Qualimap](#qualimap) - - [Sentieon WgsMetricsAlgo](#sention-wgsmetricsalgo) - - [TIDDIT's cov and UCSC WigToBigWig](#tiddits-cov-and-ucsc-wigtobigwig) - - [Reporting](#reporting) - - [MultiQC](#multiqc) -- [Variant calling - SNV](#variant-calling---snv) - - [DeepVariant](#deepvariant) - - [Sentieon DNAscope](#sentieon-dnascope) -- [Variant calling - SV](#variant-calling---sv) - - [Manta](#manta) - - [TIDDIT sv](#tiddit-sv) - - [GATK GermlineCNVCaller - CNV calling](#gatk-germlinecnvcaller---cnv-calling) - - [SVDB merge](#svdb-merge) -- [Variant calling - repeat expansions](#variant-calling---repeat-expansions) - - [Expansion Hunter](#expansion-hunter) - - [Stranger](#stranger) -- [Annotation - SNV](#annotation---snv) - - [bcftools roh](#bcftools-roh) - - [vcfanno](#vcfanno) - - [CADD](#cadd) - - [VEP](#vep) - - [UPD](#upd) - - [Chromograph](#chromograph) -- [Annotation - SV](#annotation---sv) - - [SVDB query](#svdb-query) - - [VEP](#vep-1) -- [Mitochondrial analysis](#mitochondrial-analysis) - - [Alignment and variant calling](#alignment-and-variant-calling) - - [MT deletion script](#mt-deletion-script) - - [eKLIPse](#eklipse) - - [Annotation:](#annotation-) - - [HaploGrep2](#haplogrep2) - - [vcfanno](#vcfanno-1) - - [CADD](#cadd-1) - - [VEP](#vep-2) - - [HmtNote](#hmtnote) -- [Rank variants and filtering](#rank-variants-and-filtering) - - [GENMOD](#genmod) -- [Pipeline information](#pipeline-information) +- [nf-core/raredisease: Output](#nf-coreraredisease-output) + - [Introduction](#introduction) + - [Pipeline overview](#pipeline-overview) + - [Alignment](#alignment) + - [Mapping](#mapping) + - [Bwa-mem2](#bwa-mem2) + - [Sentieon bwa mem](#sentieon-bwa-mem) + - [Duplicate marking](#duplicate-marking) + - [Picard's MarkDuplicates](#picards-markduplicates) + - [Sentieon Dedup](#sentieon-dedup) + - [Quality control and reporting](#quality-control-and-reporting) + - [Quality control](#quality-control) + - [FastQC](#fastqc) + - [Mosdepth](#mosdepth) + - [Picard tools](#picard-tools) + - [Qualimap](#qualimap) + - [Sention WgsMetricsAlgo](#sention-wgsmetricsalgo) + - [TIDDIT's cov and UCSC WigToBigWig](#tiddits-cov-and-ucsc-wigtobigwig) + - [Reporting](#reporting) + - [MultiQC](#multiqc) + - [Variant calling - SNV](#variant-calling---snv) + - [DeepVariant](#deepvariant) + - [Sentieon DNAscope](#sentieon-dnascope) + - [Variant calling - SV](#variant-calling---sv) + - [Manta](#manta) + - [TIDDIT sv](#tiddit-sv) + - [GATK GermlineCNVCaller - CNV calling](#gatk-germlinecnvcaller---cnv-calling) + - [CNVnator - CNV calling](#cnvnator---cnv-calling) + - [SVDB merge](#svdb-merge) + - [Variant calling - repeat expansions](#variant-calling---repeat-expansions) + - [Expansion Hunter](#expansion-hunter) + - [Stranger](#stranger) + - [Annotation - SNV](#annotation---snv) + - [bcftools roh](#bcftools-roh) + - [vcfanno](#vcfanno) + - [CADD](#cadd) + - [VEP](#vep) + - [UPD](#upd) + - [Chromograph](#chromograph) + - [Annotation - SV](#annotation---sv) + - [SVDB query](#svdb-query) + - [VEP](#vep-1) + - [Mitochondrial analysis](#mitochondrial-analysis) + - [Alignment and variant calling](#alignment-and-variant-calling) + - [MT deletion script](#mt-deletion-script) + - [eKLIPse](#eklipse) + - [Annotation:](#annotation) + - [HaploGrep2](#haplogrep2) + - [vcfanno](#vcfanno-1) + - [CADD](#cadd-1) + - [Hmtnote](#hmtnote) + - [VEP](#vep-2) + - [Rank variants and filtering](#rank-variants-and-filtering) + - [GENMOD](#genmod) + - [Pipeline information](#pipeline-information) ### Alignment @@ -259,9 +263,13 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support. [GATK GermlineCNVCaller](https://github.com/broadinstitute/gatk) is used to identify copy number variants in germline samples given their read counts and a model describing a sample's ploidy. Output vcf files are treated as intermediates and are not placed in the output folder. +#### CNVnator - CNV calling + +[CNVnator](https://github.com/abyzovlab/CNVnator) is used to identify copy number variants in germline samples given a bam file. Output vcf files are treated as intermediates and are not placed in the output folder. + #### SVDB merge -[SVDB merge](https://github.com/J35P312/SVDB#merge) is used to merge the variant calls from GATK's GermlineCNVCaller (only if skip_cnv_calling is set to false), Manta, and TIDDIT. Output files are published in the output folder. +[SVDB merge](https://github.com/J35P312/SVDB#merge) is used to merge the variant calls from GATK's GermlineCNVCaller (only if skip_germlinecnvcaller is set to false), Manta, CNVnator and TIDDIT. Output files are published in the output folder.

Output files diff --git a/nextflow.config b/nextflow.config index 9573e35c..fd3daca3 100644 --- a/nextflow.config +++ b/nextflow.config @@ -24,7 +24,7 @@ params { analysis_type = 'wgs' bait_padding = 100 save_mapped_as_cram = false - skip_cnv_calling = false + skip_germlinecnvcaller = false skip_snv_annotation = false skip_sv_annotation = false skip_mt_annotation = false @@ -304,6 +304,7 @@ includeConfig 'conf/modules/call_snv_MT.config' includeConfig 'conf/modules/call_snv_deepvariant.config' includeConfig 'conf/modules/call_snv_sentieon.config' includeConfig 'conf/modules/call_sv_MT.config' +includeConfig 'conf/modules/call_sv_cnvnator.config' includeConfig 'conf/modules/call_sv_germlinecnvcaller.config' includeConfig 'conf/modules/call_sv_manta.config' includeConfig 'conf/modules/call_sv_tiddit.config' diff --git a/nextflow_schema.json b/nextflow_schema.json index a3a95f1c..9ae8f9b0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -383,9 +383,9 @@ "description": "Specifies whether to generate and publish alignment files as cram instead of bam", "fa_icon": "fas fa-toggle-on" }, - "skip_cnv_calling": { + "skip_germlinecnvcaller": { "type": "boolean", - "description": "Specifies whether or not to skip CNV calling.", + "description": "Specifies whether or not to skip CNV calling using GATK's GermlineCNVCaller", "fa_icon": "fas fa-toggle-on" }, "skip_mt_annotation": { diff --git a/subworkflows/local/call_structural_variants.nf b/subworkflows/local/call_structural_variants.nf index 28f4e8db..88e0aa39 100644 --- a/subworkflows/local/call_structural_variants.nf +++ b/subworkflows/local/call_structural_variants.nf @@ -8,6 +8,7 @@ include { CALL_SV_MT as CALL_SV_MT_SHIFT } from './variant_calling/call_sv_MT' include { CALL_SV_TIDDIT } from './variant_calling/call_sv_tiddit' include { SVDB_MERGE } from '../../modules/nf-core/svdb/merge/main' include { CALL_SV_GERMLINECNVCALLER } from './variant_calling/call_sv_germlinecnvcaller' +include { CALL_SV_CNVNATOR } from './variant_calling/call_sv_cnvnator' include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix/main' workflow CALL_STRUCTURAL_VARIANTS { @@ -48,18 +49,25 @@ workflow CALL_STRUCTURAL_VARIANTS { .collect{it[1]} .set { gcnvcaller_vcf } + CALL_SV_CNVNATOR (ch_genome_bam_bai, ch_genome_fasta, ch_genome_fai, ch_case_info) + .vcf + .collect{it[1]} + .set { cnvnator_vcf } + CALL_SV_MT (ch_mt_bam_bai, ch_genome_fasta) //merge - if (params.skip_cnv_calling) { + if (params.skip_germlinecnvcaller) { tiddit_vcf .combine(manta_vcf) + .combine(cnvnator_vcf) .toList() .set { vcf_list } } else { tiddit_vcf .combine(manta_vcf) .combine(gcnvcaller_vcf) + .combine(cnvnator_vcf) .toList() .set { vcf_list } } diff --git a/subworkflows/local/variant_calling/call_sv_cnvnator.nf b/subworkflows/local/variant_calling/call_sv_cnvnator.nf new file mode 100644 index 00000000..2c6db643 --- /dev/null +++ b/subworkflows/local/variant_calling/call_sv_cnvnator.nf @@ -0,0 +1,51 @@ +// +// A subworkflow to call CNVs using cnvnator +// + +nextflow.enable.dsl = 2 + +include { CNVNATOR_CNVNATOR as CNVNATOR_RD } from '../../../modules/nf-core/cnvnator/cnvnator/main.nf' +include { CNVNATOR_CNVNATOR as CNVNATOR_HIST } from '../../../modules/nf-core/cnvnator/cnvnator/main.nf' +include { CNVNATOR_CNVNATOR as CNVNATOR_STAT } from '../../../modules/nf-core/cnvnator/cnvnator/main.nf' +include { CNVNATOR_CNVNATOR as CNVNATOR_PARTITION } from '../../../modules/nf-core/cnvnator/cnvnator/main.nf' +include { CNVNATOR_CNVNATOR as CNVNATOR_CALL } from '../../../modules/nf-core/cnvnator/cnvnator/main.nf' +include { CNVNATOR_CONVERT2VCF } from '../../../modules/nf-core/cnvnator/convert2vcf/main.nf' +include { SVDB_MERGE as SVDB_MERGE_CNVNATOR } from '../../../modules/nf-core/svdb/merge/main' + +workflow CALL_SV_CNVNATOR { + take: + ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_case_info // channel: [mandatory] [ val(case_info) ] + + main: + ch_versions = Channel.empty() + + CNVNATOR_RD ( ch_bam_bai, [[:],[]], [[:],[]], [[:],[]] ) + CNVNATOR_HIST ( [[:],[],[]], CNVNATOR_RD.out.root, ch_fasta, ch_fai ) + CNVNATOR_STAT ( [[:],[],[]], CNVNATOR_HIST.out.root, [[:],[]], [[:],[]] ) + CNVNATOR_PARTITION ( [[:],[],[]], CNVNATOR_STAT.out.root, [[:],[]], [[:],[]] ) + CNVNATOR_CALL ( [[:],[],[]], CNVNATOR_PARTITION.out.root, [[:],[]], [[:],[]] ) + CNVNATOR_CONVERT2VCF (CNVNATOR_CALL.out.tab).vcf + .collect{it[1]} + .toList() + .set { vcf_file_list } + + ch_case_info + .combine(vcf_file_list) + .set { merge_input_vcfs } + + SVDB_MERGE_CNVNATOR ( merge_input_vcfs, [] ) + + ch_versions = ch_versions.mix(CNVNATOR_RD.out.versions) + ch_versions = ch_versions.mix(CNVNATOR_HIST.out.versions) + ch_versions = ch_versions.mix(CNVNATOR_STAT.out.versions) + ch_versions = ch_versions.mix(CNVNATOR_PARTITION.out.versions) + ch_versions = ch_versions.mix(CNVNATOR_CALL.out.versions) + ch_versions = ch_versions.mix(CNVNATOR_CONVERT2VCF.out.versions) + + emit: + vcf = CNVNATOR_CONVERT2VCF.out.vcf // channel: [ val(meta), path(*.tar.gz) ] + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 95d95590..86e623e0 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -54,7 +54,7 @@ if (params.variant_caller.equals("sentieon")) { mandatoryParams += ["ml_model"] } -if (!params.skip_cnv_calling) { +if (!params.skip_germlinecnvcaller) { mandatoryParams += ["ploidy_model", "gcnvcaller_model"] } @@ -277,10 +277,10 @@ workflow RAREDISEASE { // SV caller priority - if (params.skip_cnv_calling) { - ch_svcaller_priority = Channel.value(["tiddit", "manta"]) + if (params.skip_germlinecnvcaller) { + ch_svcaller_priority = Channel.value(["tiddit", "manta", "cnvnator"]) } else { - ch_svcaller_priority = Channel.value(["tiddit", "manta", "gcnvcaller"]) + ch_svcaller_priority = Channel.value(["tiddit", "manta", "gcnvcaller", "cnvnator"]) } // Input QC From 5424fc5d36347ee3423c546c56f95884bea96088 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 16 Oct 2023 18:26:52 +0200 Subject: [PATCH 133/387] add param cnvnator_binsize --- conf/modules/call_sv_cnvnator.config | 8 ++++---- nextflow.config | 1 + nextflow_schema.json | 6 ++++++ 3 files changed, 11 insertions(+), 4 deletions(-) diff --git a/conf/modules/call_sv_cnvnator.config b/conf/modules/call_sv_cnvnator.config index 186bdc09..401c6968 100644 --- a/conf/modules/call_sv_cnvnator.config +++ b/conf/modules/call_sv_cnvnator.config @@ -19,22 +19,22 @@ process { withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_CNVNATOR:CNVNATOR_HIST" { tag = { "$meta2.id" } - ext.args = '-his 1000' + ext.args = { "-his ${params.cnvnator_binsize}" } } withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_CNVNATOR:CNVNATOR_STAT" { tag = { "$meta2.id" } - ext.args = '-stat 1000' + ext.args = { "-stat ${params.cnvnator_binsize}" } } withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_CNVNATOR:CNVNATOR_PARTITION" { tag = { "$meta2.id" } - ext.args = '-partition 1000' + ext.args = { "-partition ${params.cnvnator_binsize}" } } withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_CNVNATOR:CNVNATOR_CALL" { tag = { "$meta2.id" } - ext.args = '-call 1000' + ext.args = { "-call ${params.cnvnator_binsize}" } } withName: '.*CALL_STRUCTURAL_VARIANTS:CALL_SV_CNVNATOR:SVDB_MERGE_CNVNATOR' { diff --git a/nextflow.config b/nextflow.config index fd3daca3..7f5a802a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -38,6 +38,7 @@ params { rmdup = false // Variant calling + cnvnator_binsize = 1000 variant_caller = 'deepvariant' // sentieon Defaults diff --git a/nextflow_schema.json b/nextflow_schema.json index 9ae8f9b0..6b1de717 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -442,6 +442,12 @@ "fa_icon": "fas fa-align-center", "description": "Interval in the reference that will be used in the software" }, + "cnvnator_binsize": { + "type": "number", + "description": "Bin size for CNVnator", + "default": "1000", + "fa_icon": "fas fa-align-center" + }, "sentieon_dnascope_pcr_indel_model": { "type": "string", "default": "CONSERVATIVE", From 8bc53dc07b762736c6b9f6d77c5b1071c2165ecf Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 17 Oct 2023 15:42:22 +0200 Subject: [PATCH 134/387] fix warnings and clean up terminal logs --- conf/modules/align.config | 20 +- conf/modules/align_MT.config | 38 +-- conf/modules/align_bwamem2.config | 67 +++--- conf/modules/align_sentieon.config | 53 ++--- conf/modules/annotate_cadd.config | 36 +-- conf/modules/annotate_consequence_pli.config | 120 +++++----- conf/modules/annotate_genome_snvs.config | 195 ++++++++-------- conf/modules/annotate_mt_snvs.config | 85 ++++--- .../annotate_structural_variants.config | 84 +++---- conf/modules/call_snv_sentieon.config | 53 ++--- conf/modules/call_sv_germlinecnvcaller.config | 32 +-- conf/modules/gens.config | 20 +- conf/modules/rank_variants.config | 217 +++++++++--------- conf/test.config | 2 +- conf/test_one_sample.config | 2 +- conf/test_sentieon.config | 2 +- docs/output.md | 2 +- nextflow.config | 2 +- nextflow_schema.json | 2 +- subworkflows/local/align.nf | 63 ++--- subworkflows/local/alignment/align_MT.nf | 22 +- subworkflows/local/annotate_genome_snvs.nf | 20 +- subworkflows/local/annotate_mt_snvs.nf | 22 +- subworkflows/local/call_snv.nf | 58 +++-- .../local/call_structural_variants.nf | 15 +- workflows/raredisease.nf | 4 +- 26 files changed, 641 insertions(+), 595 deletions(-) diff --git a/conf/modules/align.config b/conf/modules/align.config index 54dae858..d55b2e19 100644 --- a/conf/modules/align.config +++ b/conf/modules/align.config @@ -18,15 +18,15 @@ process{ ] } - withName: '.*ALIGN:SAMTOOLS_VIEW' { - ext.args = { '--output-fmt cram --write-index' } - ext.prefix = { "${meta.id}_sorted_md" } - ext.when = params.save_mapped_as_cram - publishDir = [ - path: { "${params.outdir}/alignment" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - + if (params.save_mapped_as_cram) { + withName: '.*ALIGN:SAMTOOLS_VIEW' { + ext.args = { '--output-fmt cram --write-index' } + ext.prefix = { "${meta.id}_sorted_md" } + publishDir = [ + path: { "${params.outdir}/alignment" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } } diff --git a/conf/modules/align_MT.config b/conf/modules/align_MT.config index 35d91ee3..23ab1c8c 100644 --- a/conf/modules/align_MT.config +++ b/conf/modules/align_MT.config @@ -17,15 +17,20 @@ process { - withName: '.*ALIGN_MT:BWAMEM2_MEM_MT' { - ext.when = { !(params.analysis_type == "wes") && params.aligner == "bwamem2" } - ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + if (params.aligner.equals("bwamem2")) { + withName: '.*ALIGN_MT:BWAMEM2_MEM_MT' { + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.when = { !(params.analysis_type.equals("wes")) } + ext.prefix = { "${meta.id}.sorted" } + } } - withName: '.*ALIGN_MT:SENTIEON_BWAMEM_MT' { - ext.args = { "-M -K 10000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type == "wes") && params.aligner == "sentieon" } - ext.prefix = { "${meta.id}.sorted" } + if (params.aligner.equals("sentieon")) { + withName: '.*ALIGN_MT:SENTIEON_BWAMEM_MT' { + ext.args = { "-M -K 10000000 -R ${meta.read_group}" } + ext.when = { !(params.analysis_type.equals("wes"))} + ext.prefix = { "${meta.id}.sorted" } + } } withName: '.*ALIGN_MT:GATK4_MERGEBAMALIGNMENT_MT' { @@ -60,15 +65,20 @@ process { process { - withName: '.*ALIGN_MT_SHIFT:BWAMEM2_MEM_MT' { - ext.when = { !(params.analysis_type == "wes") && params.aligner == "bwamem2" } - ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + if (params.aligner.equals("bwamem2")) { + withName: '.*ALIGN_MT_SHIFT:BWAMEM2_MEM_MT' { + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.when = { !(params.analysis_type.equals("wes")) } + ext.prefix = { "${meta.id}.sorted" } + } } - withName: '.*ALIGN_MT_SHIFT:SENTIEON_BWAMEM_MT' { - ext.args = { "-M -K 10000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type == "wes") && params.aligner == "sentieon" } - ext.prefix = { "${meta.id}.sorted" } + if (params.aligner.equals("sentieon")) { + withName: '.*ALIGN_MT_SHIFT:SENTIEON_BWAMEM_MT' { + ext.args = { "-M -K 10000000 -R ${meta.read_group}" } + ext.when = { !(params.analysis_type.equals("wes"))} + ext.prefix = { "${meta.id}.sorted" } + } } withName: '.*ALIGN_MT_SHIFT:GATK4_MERGEBAMALIGNMENT_MT' { diff --git a/conf/modules/align_bwamem2.config b/conf/modules/align_bwamem2.config index 7d478222..ba92f738 100644 --- a/conf/modules/align_bwamem2.config +++ b/conf/modules/align_bwamem2.config @@ -16,39 +16,38 @@ // process { - withName: '.*ALIGN_BWAMEM2:.*' { - ext.when = params.aligner.equals("bwamem2") + if (params.aligner.equals("bwamem2")) { + + withName: '.*ALIGN:ALIGN_BWAMEM2:BWAMEM2_MEM' { + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.prefix = { "${meta.id}.sorted" } + } + + withName: '.*ALIGN:ALIGN_BWAMEM2:SAMTOOLS_STATS' { + ext.args = '-s --remove-overlaps' + } + + withName: '.*ALIGN:ALIGN_BWAMEM2:SAMTOOLS_MERGE' { + ext.prefix = { "${meta.id}_sorted_merged" } + } + + withName: '.*ALIGN:ALIGN_BWAMEM2:MARKDUPLICATES' { + ext.prefix = { "${meta.id}_sorted_md" } + publishDir = [ + enabled: !params.save_mapped_as_cram, + path: { "${params.outdir}/alignment" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*ALIGN:ALIGN_BWAMEM2:SAMTOOLS_INDEX_MARKDUP' { + publishDir = [ + enabled: !params.save_mapped_as_cram, + path: { "${params.outdir}/alignment" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } - - withName: '.*ALIGN:ALIGN_BWAMEM2:BWAMEM2_MEM' { - ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - } - - withName: '.*ALIGN:ALIGN_BWAMEM2:SAMTOOLS_STATS' { - ext.args = '-s --remove-overlaps' - } - - withName: '.*ALIGN:ALIGN_BWAMEM2:SAMTOOLS_MERGE' { - ext.prefix = { "${meta.id}_sorted_merged" } - } - - withName: '.*ALIGN:ALIGN_BWAMEM2:MARKDUPLICATES' { - ext.prefix = { "${meta.id}_sorted_md" } - publishDir = [ - enabled: !params.save_mapped_as_cram, - path: { "${params.outdir}/alignment" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*ALIGN:ALIGN_BWAMEM2:SAMTOOLS_INDEX_MARKDUP' { - publishDir = [ - enabled: !params.save_mapped_as_cram, - path: { "${params.outdir}/alignment" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - } diff --git a/conf/modules/align_sentieon.config b/conf/modules/align_sentieon.config index 3e002cb6..d5156e58 100644 --- a/conf/modules/align_sentieon.config +++ b/conf/modules/align_sentieon.config @@ -17,33 +17,30 @@ process { - withName: '.*ALIGN_SENTIEON:.*' { - ext.when = params.aligner.equals("sentieon") + if (params.aligner.equals("sentieon")) { + withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_BWAMEM' { + ext.args = { "-M -K 10000000 -R ${meta.read_group} " } + ext.prefix = { "${meta.id}.sorted" } + } + + withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_DATAMETRICS' { + ext.prefix = { "${meta.id}_datametrics" } + } + + withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_READWRITER' { + ext.prefix = { "${meta.id}_merged" } + } + + withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_DEDUP' { + ext.args = { $params.rmdup ? "--rmdup" : '' } + ext.prefix = { "${meta.id}_dedup" } + ext.suffix = ".bam" + publishDir = [ + enabled: !params.save_mapped_as_cram, + path: { "${params.outdir}/alignment" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } - - withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_BWAMEM' { - ext.args = { "-M -K 10000000 -R ${meta.read_group} " } - ext.prefix = { "${meta.id}.sorted" } - } - - withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_DATAMETRICS' { - ext.prefix = { "${meta.id}_datametrics" } - } - - withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_READWRITER' { - ext.prefix = { "${meta.id}_merged" } - } - - withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_DEDUP' { - ext.args = { $params.rmdup ? "--rmdup" : '' } - ext.prefix = { "${meta.id}_dedup" } - ext.suffix = ".bam" - publishDir = [ - enabled: !params.save_mapped_as_cram, - path: { "${params.outdir}/alignment" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - } diff --git a/conf/modules/annotate_cadd.config b/conf/modules/annotate_cadd.config index 2535f7f9..5cba1d6a 100644 --- a/conf/modules/annotate_cadd.config +++ b/conf/modules/annotate_cadd.config @@ -16,26 +16,28 @@ // process { - withName: '.*:ANNOTATE_CADD.*' { - ext.when = { (params.cadd_resources != null) && ( !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun) } - } + if (params.cadd_resources != null) { + withName: '.*:ANNOTATE_CADD.*' { + ext.when = { ( !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun) } + } - withName: '.*:ANNOTATE_CADD:BCFTOOLS_VIEW' { - ext.args = { "--output-type z --types indels" } - ext.prefix = { "${vcf.simpleName}_indels" } - } + withName: '.*:ANNOTATE_CADD:BCFTOOLS_VIEW' { + ext.args = { "--output-type z --types indels" } + ext.prefix = { "${vcf.simpleName}_indels" } + } - withName: '.*:ANNOTATE_CADD:CADD' { - ext.args = { "-g ${params.genome}" } - ext.prefix = { "${vcf.simpleName}_cadd" } - } + withName: '.*:ANNOTATE_CADD:CADD' { + ext.args = { "-g ${params.genome}" } + ext.prefix = { "${vcf.simpleName}_cadd" } + } - withName: '.*:ANNOTATE_CADD:TABIX_CADD' { - ext.args = { "--force --sequence 1 --begin 2 --end 2" } - } + withName: '.*:ANNOTATE_CADD:TABIX_CADD' { + ext.args = { "--force --sequence 1 --begin 2 --end 2" } + } - withName: '.*:ANNOTATE_CADD:BCFTOOLS_ANNOTATE' { - ext.args = { "--columns Chrom,Pos,Ref,Alt,-,CADD --output-type z" } - ext.prefix = { "${input.simpleName}_ann" } + withName: '.*:ANNOTATE_CADD:BCFTOOLS_ANNOTATE' { + ext.args = { "--columns Chrom,Pos,Ref,Alt,-,CADD --output-type z" } + ext.prefix = { "${input.simpleName}_ann" } + } } } diff --git a/conf/modules/annotate_consequence_pli.config b/conf/modules/annotate_consequence_pli.config index 68b11951..7fde1038 100644 --- a/conf/modules/annotate_consequence_pli.config +++ b/conf/modules/annotate_consequence_pli.config @@ -13,80 +13,84 @@ process { - withName: '.*ANN_CSQ_PLI_SV:.*' { - ext.when = !params.skip_sv_annotation - publishDir = [ - enabled: false - ] - } + if (!params.skip_sv_annotation) { + withName: '.*ANN_CSQ_PLI_SV:.*' { + publishDir = [ + enabled: false + ] + } - withName: '.*ANN_CSQ_PLI_SV:ADD_MOST_SEVERE_CSQ' { - ext.prefix = { "${meta.id}_genome_vep_csq" } - } + withName: '.*ANN_CSQ_PLI_SV:ADD_MOST_SEVERE_CSQ' { + ext.prefix = { "${meta.id}_genome_vep_csq" } + } - withName: '.*ANN_CSQ_PLI_SV:ADD_MOST_SEVERE_PLI' { - ext.prefix = { "${meta.id}_genome_vep_csq_pli" } - } + withName: '.*ANN_CSQ_PLI_SV:ADD_MOST_SEVERE_PLI' { + ext.prefix = { "${meta.id}_genome_vep_csq_pli" } + } - withName: '.*ANN_CSQ_PLI_SV:TABIX_BGZIPTABIX' { - ext.prefix = { "${meta.id}_genome_vep_csq_pli" } - publishDir = [ - path: { "${params.outdir}/annotate_sv" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + withName: '.*ANN_CSQ_PLI_SV:TABIX_BGZIPTABIX' { + ext.prefix = { "${meta.id}_genome_vep_csq_pli" } + publishDir = [ + path: { "${params.outdir}/annotate_sv" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } - } process { - withName: '.*ANN_CSQ_PLI_SNV:.*' { - ext.when = !params.skip_snv_annotation - publishDir = [ - enabled: false - ] - } + if (!params.skip_snv_annotation) { + withName: '.*ANN_CSQ_PLI_SNV:.*' { + ext.when = !params.skip_snv_annotation + publishDir = [ + enabled: false + ] + } - withName: '.*ANN_CSQ_PLI_SNV:ADD_MOST_SEVERE_CSQ' { - ext.prefix = { "${meta.id}_vep_csq" } - } + withName: '.*ANN_CSQ_PLI_SNV:ADD_MOST_SEVERE_CSQ' { + ext.prefix = { "${meta.id}_vep_csq" } + } - withName: '.*ANN_CSQ_PLI_SNV:ADD_MOST_SEVERE_PLI' { - ext.prefix = { "${meta.id}_vep_csq_pli" } - } + withName: '.*ANN_CSQ_PLI_SNV:ADD_MOST_SEVERE_PLI' { + ext.prefix = { "${meta.id}_vep_csq_pli" } + } - withName: '.*ANN_CSQ_PLI_SNV:TABIX_BGZIPTABIX' { - ext.prefix = { "${meta.id}_vep_csq_pli" } - publishDir = [ - path: { "${params.outdir}/annotate_snv/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + withName: '.*ANN_CSQ_PLI_SNV:TABIX_BGZIPTABIX' { + ext.prefix = { "${meta.id}_vep_csq_pli" } + publishDir = [ + path: { "${params.outdir}/annotate_snv/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } } process { - withName: '.*ANN_CSQ_PLI_MT:.*' { - ext.when = !params.skip_mt_annotation - publishDir = [ - enabled: false - ] - } + if (!params.skip_mt_annotation) { + withName: '.*ANN_CSQ_PLI_MT:.*' { + ext.when = !params.skip_mt_annotation + publishDir = [ + enabled: false + ] + } - withName: '.*ANN_CSQ_PLI_MT:ADD_MOST_SEVERE_CSQ' { - ext.prefix = { "${meta.id}_mt_vep_csq" } - } + withName: '.*ANN_CSQ_PLI_MT:ADD_MOST_SEVERE_CSQ' { + ext.prefix = { "${meta.id}_mt_vep_csq" } + } - withName: '.*ANN_CSQ_PLI_MT:ADD_MOST_SEVERE_PLI' { - ext.prefix = { "${meta.id}_mt_vep_csq_pli" } - } + withName: '.*ANN_CSQ_PLI_MT:ADD_MOST_SEVERE_PLI' { + ext.prefix = { "${meta.id}_mt_vep_csq_pli" } + } - withName: '.*ANN_CSQ_PLI_MT:TABIX_BGZIPTABIX' { - ext.prefix = { "${meta.id}_mt_vep_csq_pli" } - publishDir = [ - path: { "${params.outdir}/annotate_snv/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + withName: '.*ANN_CSQ_PLI_MT:TABIX_BGZIPTABIX' { + ext.prefix = { "${meta.id}_mt_vep_csq_pli" } + publishDir = [ + path: { "${params.outdir}/annotate_snv/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } } diff --git a/conf/modules/annotate_genome_snvs.config b/conf/modules/annotate_genome_snvs.config index f65d24fd..a68d83e9 100644 --- a/conf/modules/annotate_genome_snvs.config +++ b/conf/modules/annotate_genome_snvs.config @@ -16,108 +16,99 @@ // process { - withName: '.*ANNOTATE_GENOME_SNVS:.*' { - ext.when = !params.skip_snv_annotation - } - - withName: '.*ANNOTATE_GENOME_SNVS:VCFANNO' { - ext.prefix = { "${meta.id}_vcfanno" } - publishDir = [ - enabled: false - ] - } - - withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_ROH' { - ext.args = { "--samples ${meta.probands.join(",")} --skip-indels " } - ext.prefix = { "${meta.id}_roh" } - } - - withName: '.*ANNOTATE_GENOME_SNVS:RHOCALL_ANNOTATE' { - ext.args = { "--v14 " } - ext.prefix = { "${meta.id}_rohann" } - } - - withName: '.*ANNOTATE_GENOME_SNVS:VCFANNO' { - ext.prefix = { "${meta.id}_rohann_vcfanno" } - } - - withName: '.*ANNOTATE_GENOME_SNVS:UPD_SITES' { - ext.prefix = { "${meta.id}_rohann_vcfanno_upd_sites" } - ext.args = {"--af-tag GNOMADAF --proband ${meta.upd_child} --mother ${meta.mother} --father ${meta.father} sites"} - } - - withName: '.*ANNOTATE_GENOME_SNVS:UPD_REGIONS' { - ext.prefix = { "${meta.id}_rohann_vcfanno_upd_regions" } - ext.args = {"--af-tag GNOMADAF --proband ${meta.upd_child} --mother ${meta.mother} --father ${meta.father} regions --min-size 5 --min-sites 1"} - ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun } - } - withName: '.*ANNOTATE_GENOME_SNVS:CHROMOGRAPH_SITES' { - ext.prefix = { "${meta7.id}_rohann_vcfanno_upd_sites_chromograph" } - ext.args = { "--euploid" } - tag = {"${meta7.id}"} - publishDir = [ - path: { "${params.outdir}/annotate_snv/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + if (!params.skip_snv_annotation) { + withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_ROH' { + ext.args = { "--samples ${meta.probands.join(",")} --skip-indels " } + ext.prefix = { "${meta.id}_roh" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:RHOCALL_ANNOTATE' { + ext.args = { "--v14 " } + ext.prefix = { "${meta.id}_rohann" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:VCFANNO' { + ext.prefix = { "${meta.id}_rohann_vcfanno" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:UPD_SITES' { + ext.prefix = { "${meta.id}_rohann_vcfanno_upd_sites" } + ext.args = {"--af-tag GNOMADAF --proband ${meta.upd_child} --mother ${meta.mother} --father ${meta.father} sites"} + } + + withName: '.*ANNOTATE_GENOME_SNVS:UPD_REGIONS' { + ext.prefix = { "${meta.id}_rohann_vcfanno_upd_regions" } + ext.args = {"--af-tag GNOMADAF --proband ${meta.upd_child} --mother ${meta.mother} --father ${meta.father} regions --min-size 5 --min-sites 1"} + ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun } + } + + withName: '.*ANNOTATE_GENOME_SNVS:CHROMOGRAPH_SITES' { + ext.prefix = { "${meta7.id}_rohann_vcfanno_upd_sites_chromograph" } + ext.args = { "--euploid" } + tag = {"${meta7.id}"} + publishDir = [ + path: { "${params.outdir}/annotate_snv/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*ANNOTATE_GENOME_SNVS:CHROMOGRAPH_REGIONS' { + ext.prefix = { "${meta6.id}_rohann_vcfanno_upd_regions_chromograph" } + ext.args = { '--euploid' } + ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample', 'test_sentieon']).size() >= 1) || workflow.stubRun } + tag = {"${meta6.id}"} + publishDir = [ + path: { "${params.outdir}/annotate_snv/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_VIEW' { + ext.prefix = { "${meta.id}_rohann_vcfanno_filter" } + ext.args = { '--output-type z --exclude "INFO/GNOMADAF > 0.70 | INFO/GNOMADAF_popmax > 0.70" ' } + } + + withName: '.*ANNOTATE_GENOME_SNVS:GATK4_SELECTVARIANTS' { + ext.prefix = { "${meta.id}_${intervals.simpleName}" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:ENSEMBLVEP_SNV' { + ext.prefix = { "${vcf.simpleName}_rohann_vcfanno_filter_vep" } + ext.args = [ + '--dir_plugins vep_cache/Plugins', + '--plugin LoFtool,vep_cache/LoFtool_scores.txt', + '--plugin pLI,vep_cache/pLI_values_107.txt', + '--plugin SpliceAI,snv=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', + '--plugin MaxEntScan,vep_cache/fordownload,SWA,NCSS', + '--distance 5000', + '--buffer_size 20000', + '--format vcf --max_sv_size 248956422', + '--appris --biotype --cache --canonical --ccds --compress_output bgzip', + '--domains --exclude_predicted --force_overwrite', + '--hgvs --humdiv --no_progress --no_stats --numbers', + '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl', + '--uniprot --vcf' + ].join(' ') + } + + withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_CONCAT' { + ext.prefix = { "${meta.id}_rohann_vcfanno_filter_vep" } + publishDir = [ + path: { "${params.outdir}/annotate_snv/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*ANNOTATE_GENOME_SNVS:TABIX_BCFTOOLS_CONCAT' { + publishDir = [ + path: { "${params.outdir}/annotate_snv/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } - - withName: '.*ANNOTATE_GENOME_SNVS:CHROMOGRAPH_REGIONS' { - ext.prefix = { "${meta6.id}_rohann_vcfanno_upd_regions_chromograph" } - ext.args = { '--euploid' } - ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample', 'test_sentieon']).size() >= 1) || workflow.stubRun } - tag = {"${meta6.id}"} - publishDir = [ - path: { "${params.outdir}/annotate_snv/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_VIEW' { - ext.prefix = { "${meta.id}_rohann_vcfanno_filter" } - ext.args = { '--output-type z --exclude "INFO/GNOMADAF > 0.70 | INFO/GNOMADAF_popmax > 0.70" ' } - } - - withName: '.*ANNOTATE_GENOME_SNVS:GATK4_SELECTVARIANTS' { - ext.prefix = { "${meta.id}_${intervals.simpleName}" } - } - - withName: '.*ANNOTATE_GENOME_SNVS:ENSEMBLVEP_SNV' { - ext.prefix = { "${vcf.simpleName}_rohann_vcfanno_filter_vep" } - ext.args = [ - '--dir_plugins vep_cache/Plugins', - '--plugin LoFtool,vep_cache/LoFtool_scores.txt', - '--plugin pLI,vep_cache/pLI_values_107.txt', - '--plugin SpliceAI,snv=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', - '--plugin MaxEntScan,vep_cache/fordownload,SWA,NCSS', - '--distance 5000', - '--buffer_size 20000', - '--format vcf --max_sv_size 248956422', - '--appris --biotype --cache --canonical --ccds --compress_output bgzip', - '--domains --exclude_predicted --force_overwrite', - '--hgvs --humdiv --no_progress --no_stats --numbers', - '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl', - '--uniprot --vcf' - ].join(' ') - } - - withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_CONCAT' { - ext.prefix = { "${meta.id}_rohann_vcfanno_filter_vep" } - publishDir = [ - path: { "${params.outdir}/annotate_snv/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*ANNOTATE_GENOME_SNVS:TABIX_BCFTOOLS_CONCAT' { - publishDir = [ - path: { "${params.outdir}/annotate_snv/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - } diff --git a/conf/modules/annotate_mt_snvs.config b/conf/modules/annotate_mt_snvs.config index 40101033..391a3e71 100644 --- a/conf/modules/annotate_mt_snvs.config +++ b/conf/modules/annotate_mt_snvs.config @@ -16,51 +16,50 @@ // process { + if (!params.skip_mt_annotation) { + withName: '.*ANNOTATE_MT_SNVS:ENSEMBLVEP_MT' { + ext.args = [ + '--dir_plugins vep_cache/Plugins', + '--plugin LoFtool,vep_cache/LoFtool_scores.txt', + '--plugin pLI,vep_cache/pLI_values_107.txt', + '--plugin SpliceAI,snv=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', + '--plugin MaxEntScan,vep_cache/fordownload,SWA,NCSS', + '--distance 0', + '--buffer_size 20000', + '--format vcf --fork 4 --max_sv_size 248956422', + '--appris --biotype --cache --canonical --ccds --compress_output bgzip', + '--domains --exclude_predicted --force_overwrite', + '--hgvs --humdiv --no_progress --no_stats --numbers', + '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl --vcf', + '--uniprot' + ].join(' ') + } + withName: '.*ANNOTATE_MT_SNVS:ZIP_TABIX_HMTNOTE' { + ext.prefix = { "${meta.id}_vep_vcfanno_hmtnote_mt" } + publishDir = [ + path: { "${params.outdir}/annotate_snv/mitochondria" }, + mode: params.publish_dir_mode, + pattern: "*{vcf.gz,vcf.gz.tbi}", + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } - withName: '.*ANNOTATE_MT_SNVS:ENSEMBLVEP_MT' { - ext.args = [ - '--dir_plugins vep_cache/Plugins', - '--plugin LoFtool,vep_cache/LoFtool_scores.txt', - '--plugin pLI,vep_cache/pLI_values_107.txt', - '--plugin SpliceAI,snv=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', - '--plugin MaxEntScan,vep_cache/fordownload,SWA,NCSS', - '--distance 0', - '--buffer_size 20000', - '--format vcf --fork 4 --max_sv_size 248956422', - '--appris --biotype --cache --canonical --ccds --compress_output bgzip', - '--domains --exclude_predicted --force_overwrite', - '--hgvs --humdiv --no_progress --no_stats --numbers', - '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl --vcf', - '--uniprot' - ].join(' ') - } - - withName: '.*ANNOTATE_MT_SNVS:ZIP_TABIX_HMTNOTE' { - ext.prefix = { "${meta.id}_vep_vcfanno_hmtnote_mt" } - publishDir = [ - path: { "${params.outdir}/annotate_snv/mitochondria" }, - mode: params.publish_dir_mode, - pattern: "*{vcf.gz,vcf.gz.tbi}", - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*ANNOTATE_MT_SNVS:HMTNOTE_ANNOTATE' { + ext.prefix = { "${meta.id}_vep_vcfanno_hmtnote_mt" } + ext.args = '--offline' + publishDir = [ + enabled: false + ] + } - withName: '.*ANNOTATE_MT_SNVS:HMTNOTE_ANNOTATE' { - ext.prefix = { "${meta.id}_vep_vcfanno_hmtnote_mt" } - ext.args = '--offline' - publishDir = [ - enabled: false - ] + withName: '.*ANNOTATE_MT_SNVS:HAPLOGREP2_CLASSIFY_MT' { + ext.prefix = { "${meta.id}_haplogrep" } + publishDir = [ + path: { "${params.outdir}/annotate_snv/mitochondria" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } - - withName: '.*ANNOTATE_MT_SNVS:HAPLOGREP2_CLASSIFY_MT' { - ext.prefix = { "${meta.id}_haplogrep" } - publishDir = [ - path: { "${params.outdir}/annotate_snv/mitochondria" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - } diff --git a/conf/modules/annotate_structural_variants.config b/conf/modules/annotate_structural_variants.config index a951172e..4b3dfe7a 100644 --- a/conf/modules/annotate_structural_variants.config +++ b/conf/modules/annotate_structural_variants.config @@ -16,51 +16,53 @@ // process { - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:.*' { - ext.when = !params.skip_sv_annotation - publishDir = [ - enabled: false - ] - } - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:SVDB_QUERY' { - ext.prefix = { "${meta.id}_svdbquery" } - } + if (!params.skip_sv_annotation) { + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:.*' { + publishDir = [ + enabled: false + ] + } - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:PICARD_SORTVCF' { - ext.prefix = { "${meta.id}_sortvcf" } - } + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:SVDB_QUERY' { + ext.prefix = { "${meta.id}_svdbquery" } + } - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:BCFTOOLS_VIEW' { - ext.prefix = { "${meta.id}_view" } - } + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:PICARD_SORTVCF' { + ext.prefix = { "${meta.id}_sortvcf" } + } - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:ENSEMBLVEP_SV' { - ext.args = [ - '--dir_cache vep_cache', - '--dir_plugins vep_cache/Plugins', - '--plugin pLI,vep_cache/pLI_values_107.txt', - '--appris --biotype --buffer_size 100 --canonical --cache --ccds', - '--compress_output bgzip --distance 5000 --domains', - '--exclude_predicted --force_overwrite --format vcf', - '--fork 4 --hgvs --humdiv --max_sv_size 248956422 --merged', - '--no_progress --no_stats --numbers --per_gene --polyphen p', - '--protein --offline --regulatory --sift p', - '--symbol --tsl --uniprot --vcf' - ].join(' ') - ext.prefix = { "${meta.id}_svdbquery_vep" } - publishDir = [ - path: { "${params.outdir}/annotate_sv" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:BCFTOOLS_VIEW' { + ext.prefix = { "${meta.id}_view" } + } + + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:ENSEMBLVEP_SV' { + ext.args = [ + '--dir_cache vep_cache', + '--dir_plugins vep_cache/Plugins', + '--plugin pLI,vep_cache/pLI_values_107.txt', + '--appris --biotype --buffer_size 100 --canonical --cache --ccds', + '--compress_output bgzip --distance 5000 --domains', + '--exclude_predicted --force_overwrite --format vcf', + '--fork 4 --hgvs --humdiv --max_sv_size 248956422 --merged', + '--no_progress --no_stats --numbers --per_gene --polyphen p', + '--protein --offline --regulatory --sift p', + '--symbol --tsl --uniprot --vcf' + ].join(' ') + ext.prefix = { "${meta.id}_svdbquery_vep" } + publishDir = [ + path: { "${params.outdir}/annotate_sv" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:TABIX_VEP' { - publishDir = [ - path: { "${params.outdir}/annotate_sv" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:TABIX_VEP' { + publishDir = [ + path: { "${params.outdir}/annotate_sv" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } } diff --git a/conf/modules/call_snv_sentieon.config b/conf/modules/call_snv_sentieon.config index 55751d4d..3abcd31c 100644 --- a/conf/modules/call_snv_sentieon.config +++ b/conf/modules/call_snv_sentieon.config @@ -17,39 +17,36 @@ process { - withName: '.*CALL_SNV_SENTIEON:.*' { - ext.when = params.variant_caller.equals("sentieon") - } - - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SENTIEON_DNASCOPE' { - ext.prefix = { "${meta.id}_dnascope" } - ext.args2 = { - params.variant_type ? "--var_type ${params.variant_type}" : '' + if(params.variant_caller.equals("sentieon")) { + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SENTIEON_DNASCOPE' { + ext.prefix = { "${meta.id}_dnascope" } + ext.args2 = { + params.variant_type ? "--var_type ${params.variant_type}" : '' + } } - } - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SENTIEON_DNAMODELAPPLY' { - ext.prefix = { "${meta.id}_dnamodelapply" } - } + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SENTIEON_DNAMODELAPPLY' { + ext.prefix = { "${meta.id}_dnamodelapply" } + } - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:BCF_FILTER_ONE' { - ext.args = "-s 'ML_FAIL' -i 'INFO/ML_PROB <= 0.95' -m x -Oz" - ext.prefix = { "${meta.id}_mlfiltered_0.95" } - } + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:BCF_FILTER_ONE' { + ext.args = "-s 'ML_FAIL' -i 'INFO/ML_PROB <= 0.95' -m x -Oz" + ext.prefix = { "${meta.id}_mlfiltered_0.95" } + } - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:BCF_FILTER_TWO' { - ext.args = "-i FILTER='\"PASS\"' -m x -Oz" - ext.prefix = { "${meta.id}_passed" } - } + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:BCF_FILTER_TWO' { + ext.args = "-i FILTER='\"PASS\"' -m x -Oz" + ext.prefix = { "${meta.id}_passed" } + } - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SPLIT_MULTIALLELICS_SEN' { - ext.args = '--output-type z --multiallelics -both' - ext.prefix = { "${meta.id}_split" } - } + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SPLIT_MULTIALLELICS_SEN' { + ext.args = '--output-type z --multiallelics -both' + ext.prefix = { "${meta.id}_split" } + } - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:REMOVE_DUPLICATES_SEN' { - ext.args = '--output-type z --rm-dup none' - ext.prefix = { "${meta.id}_split_rmdup" } + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:REMOVE_DUPLICATES_SEN' { + ext.args = '--output-type z --rm-dup none' + ext.prefix = { "${meta.id}_split_rmdup" } + } } - } diff --git a/conf/modules/call_sv_germlinecnvcaller.config b/conf/modules/call_sv_germlinecnvcaller.config index bf37b9d9..8539e546 100644 --- a/conf/modules/call_sv_germlinecnvcaller.config +++ b/conf/modules/call_sv_germlinecnvcaller.config @@ -17,23 +17,25 @@ process { - withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER.*" { - publishDir = [ - enabled: false - ] - ext.when = !params.skip_cnv_calling - } + if (!params.skip_germlinecnvcaller) { + withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER.*" { + publishDir = [ + enabled: false + ] + ext.when = !params.skip_germlinecnvcaller + } - withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:GATK4_COLLECTREADCOUNTS" { - ext.args = "--format TSV --interval-merging-rule OVERLAPPING_ONLY" - } + withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:GATK4_COLLECTREADCOUNTS" { + ext.args = "--format TSV --interval-merging-rule OVERLAPPING_ONLY" + } - withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:GATK4_DETERMINEGERMLINECONTIGPLOIDY" { - ext.prefix = { "${meta.id}_ploidy" } - } + withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:GATK4_DETERMINEGERMLINECONTIGPLOIDY" { + ext.prefix = { "${meta.id}_ploidy" } + } - withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:GATK4_GERMLINECNVCALLER" { - ext.args = "--run-mode CASE" - ext.prefix = { "${meta.id}_${model.simpleName}" } + withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:GATK4_GERMLINECNVCALLER" { + ext.args = "--run-mode CASE" + ext.prefix = { "${meta.id}_${model.simpleName}" } + } } } diff --git a/conf/modules/gens.config b/conf/modules/gens.config index b918a6b2..3a3f6ccf 100644 --- a/conf/modules/gens.config +++ b/conf/modules/gens.config @@ -16,15 +16,17 @@ // process { - withName: '.*GENS:.*' { - publishDir = [ - path: { "${params.outdir}/gens" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + if (params.gens_switch) { + withName: '.*GENS:.*' { + publishDir = [ + path: { "${params.outdir}/gens" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } - withName: '.*GENS:COLLECTREADCOUNTS' { - ext.args = '--interval-merging-rule OVERLAPPING_ONLY' + withName: '.*GENS:COLLECTREADCOUNTS' { + ext.args = '--interval-merging-rule OVERLAPPING_ONLY' + } } } diff --git a/conf/modules/rank_variants.config b/conf/modules/rank_variants.config index f5882da1..65da5cc3 100644 --- a/conf/modules/rank_variants.config +++ b/conf/modules/rank_variants.config @@ -16,47 +16,48 @@ // process { - withName: '.*RANK_VARIANTS_SV:.*' { - ext.when = !params.skip_sv_annotation - publishDir = [ - enabled: false - ] - } + if (!params.skip_sv_annotation) { + withName: '.*RANK_VARIANTS_SV:.*' { + publishDir = [ + enabled: false + ] + } - withName: '.*RANK_VARIANTS_SV:GENMOD_ANNOTATE' { - ext.args = { - (params.genome == 'GRCh37') ? '--annotate_regions --genome-build 37' : '--annotate_regions --genome-build 38' + withName: '.*RANK_VARIANTS_SV:GENMOD_ANNOTATE' { + ext.args = { + (params.genome == 'GRCh37') ? '--annotate_regions --genome-build 37' : '--annotate_regions --genome-build 38' + } } - } - withName: '.*RANK_VARIANTS_SV:GENMOD_SCORE' { - ext.args = " --rank_results " - } + withName: '.*RANK_VARIANTS_SV:GENMOD_SCORE' { + ext.args = " --rank_results " + } - withName: '.*RANK_VARIANTS_SV:GENMOD_COMPOUND' { - ext.prefix = { "${meta.id}_ranked_sv" } - } + withName: '.*RANK_VARIANTS_SV:GENMOD_COMPOUND' { + ext.prefix = { "${meta.id}_ranked_sv" } + } - withName: '.*RANK_VARIANTS_SV:BCFTOOLS_SORT' { - ext.args = "--output-type z" - ext.prefix = { "${meta.id}_ranked_sv" } - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*RANK_VARIANTS_SV:BCFTOOLS_SORT' { + ext.args = "--output-type z" + ext.prefix = { "${meta.id}_ranked_sv" } + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } - withName: '.*RANK_VARIANTS_SV:TABIX_BGZIP' { - ext.when = false - } + withName: '.*RANK_VARIANTS_SV:TABIX_BGZIP' { + ext.when = false + } - withName: '.*RANK_VARIANTS_SV:TABIX_TABIX' { - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + withName: '.*RANK_VARIANTS_SV:TABIX_TABIX' { + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } } @@ -65,50 +66,52 @@ process { // process { - withName: '.*RANK_VARIANTS_SNV:.*' { - ext.when = !params.skip_snv_annotation - publishDir = [ - enabled: false - ] - } + if (!params.skip_snv_annotation) { + withName: '.*RANK_VARIANTS_SNV:.*' { + ext.when = !params.skip_snv_annotation + publishDir = [ + enabled: false + ] + } - withName: '.*RANK_VARIANTS_SNV:GENMOD_ANNOTATE' { - ext.args = { - (params.genome == 'GRCh37') ? '--annotate_regions --genome-build 37' : '--annotate_regions --genome-build 38' + withName: '.*RANK_VARIANTS_SNV:GENMOD_ANNOTATE' { + ext.args = { + (params.genome == 'GRCh37') ? '--annotate_regions --genome-build 37' : '--annotate_regions --genome-build 38' + } } - } - withName: '.*RANK_VARIANTS_SNV:GENMOD_MODELS' { - ext.args = " --whole_gene " - } + withName: '.*RANK_VARIANTS_SNV:GENMOD_MODELS' { + ext.args = " --whole_gene " + } - withName: '.*RANK_VARIANTS_SNV:GENMOD_SCORE' { - ext.args = " --rank_results " - } + withName: '.*RANK_VARIANTS_SNV:GENMOD_SCORE' { + ext.args = " --rank_results " + } - withName: '.*RANK_VARIANTS_SNV:GENMOD_COMPOUND' { - ext.prefix = { "${meta.id}_ranked_snv" } - } + withName: '.*RANK_VARIANTS_SNV:GENMOD_COMPOUND' { + ext.prefix = { "${meta.id}_ranked_snv" } + } - withName: '.*RANK_VARIANTS_SNV:BCFTOOLS_SORT' { - ext.when = false - } + withName: '.*RANK_VARIANTS_SNV:BCFTOOLS_SORT' { + ext.when = false + } - withName: '.*RANK_VARIANTS_SNV:TABIX_BGZIP' { - ext.prefix = { "${meta.id}_ranked_snv" } - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*RANK_VARIANTS_SNV:TABIX_BGZIP' { + ext.prefix = { "${meta.id}_ranked_snv" } + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } - withName: '.*RANK_VARIANTS_SNV:TABIX_TABIX' { - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + withName: '.*RANK_VARIANTS_SNV:TABIX_TABIX' { + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } } @@ -117,49 +120,51 @@ process { // process { - withName: '.*RANK_VARIANTS_MT:.*' { - ext.when = !params.skip_snv_annotation - publishDir = [ - enabled: false - ] - } + if (!params.skip_mt_annotation) { + withName: '.*RANK_VARIANTS_MT:.*' { + ext.when = !params.skip_snv_annotation + publishDir = [ + enabled: false + ] + } - withName: '.*RANK_VARIANTS_MT:GENMOD_ANNOTATE' { - ext.args = { - (params.genome == 'GRCh37') ? '--annotate_regions --genome-build 37' : '--annotate_regions --genome-build 38' + withName: '.*RANK_VARIANTS_MT:GENMOD_ANNOTATE' { + ext.args = { + (params.genome == 'GRCh37') ? '--annotate_regions --genome-build 37' : '--annotate_regions --genome-build 38' + } } - } - withName: '.*RANK_VARIANTS_MT:GENMOD_MODELS' { - ext.args = " --whole_gene " - } + withName: '.*RANK_VARIANTS_MT:GENMOD_MODELS' { + ext.args = " --whole_gene " + } - withName: '.*RANK_VARIANTS_MT:GENMOD_SCORE' { - ext.args = " --rank_results " - } + withName: '.*RANK_VARIANTS_MT:GENMOD_SCORE' { + ext.args = " --rank_results " + } - withName: '.*RANK_VARIANTS_MT:GENMOD_COMPOUND' { - ext.prefix = { "${meta.id}_ranked_mt" } - } + withName: '.*RANK_VARIANTS_MT:GENMOD_COMPOUND' { + ext.prefix = { "${meta.id}_ranked_mt" } + } - withName: '.*RANK_VARIANTS_MT:BCFTOOLS_SORT' { - ext.when = false - } + withName: '.*RANK_VARIANTS_MT:BCFTOOLS_SORT' { + ext.when = false + } - withName: '.*RANK_VARIANTS_MT:TABIX_BGZIP' { - ext.prefix = { "${meta.id}_ranked_mt" } - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*RANK_VARIANTS_MT:TABIX_BGZIP' { + ext.prefix = { "${meta.id}_ranked_mt" } + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } - withName: '.*RANK_VARIANTS_MT:TABIX_TABIX' { - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + withName: '.*RANK_VARIANTS_MT:TABIX_TABIX' { + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } } diff --git a/conf/test.config b/conf/test.config index c7593f76..5fbde3f4 100644 --- a/conf/test.config +++ b/conf/test.config @@ -24,7 +24,7 @@ params { mito_name = 'MT' // analysis params - skip_cnv_calling = true + skip_germlinecnvcaller = true skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI // Input data diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index 51524fe7..dba0420b 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -24,7 +24,7 @@ params { mito_name = 'MT' // analysis params - skip_cnv_calling = true + skip_germlinecnvcaller = true skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI // Input data diff --git a/conf/test_sentieon.config b/conf/test_sentieon.config index f729c35e..64618d12 100644 --- a/conf/test_sentieon.config +++ b/conf/test_sentieon.config @@ -24,7 +24,7 @@ params { mito_name = 'MT' // analysis params - skip_cnv_calling = true + skip_germlinecnvcaller = true // Input data input = 'https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/testdata/samplesheet_trio.csv' diff --git a/docs/output.md b/docs/output.md index ef1c02a9..6f3b5210 100644 --- a/docs/output.md +++ b/docs/output.md @@ -261,7 +261,7 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support. #### SVDB merge -[SVDB merge](https://github.com/J35P312/SVDB#merge) is used to merge the variant calls from GATK's GermlineCNVCaller (only if skip_cnv_calling is set to false), Manta, and TIDDIT. Output files are published in the output folder. +[SVDB merge](https://github.com/J35P312/SVDB#merge) is used to merge the variant calls from GATK's GermlineCNVCaller (only if skip_germlinecnvcaller is set to false), Manta, and TIDDIT. Output files are published in the output folder.
Output files diff --git a/nextflow.config b/nextflow.config index 9573e35c..6e05cf0d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -24,7 +24,7 @@ params { analysis_type = 'wgs' bait_padding = 100 save_mapped_as_cram = false - skip_cnv_calling = false + skip_germlinecnvcaller = false skip_snv_annotation = false skip_sv_annotation = false skip_mt_annotation = false diff --git a/nextflow_schema.json b/nextflow_schema.json index a3a95f1c..e5d2597b 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -383,7 +383,7 @@ "description": "Specifies whether to generate and publish alignment files as cram instead of bam", "fa_icon": "fas fa-toggle-on" }, - "skip_cnv_calling": { + "skip_germlinecnvcaller": { "type": "boolean", "description": "Specifies whether or not to skip CNV calling.", "fa_icon": "fas fa-toggle-on" diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index 17a6252b..8e9baa2f 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -25,26 +25,38 @@ workflow ALIGN { val_platform // string: [mandatory] illumina or a different technology main: - ch_versions = Channel.empty() + ch_versions = Channel.empty() + ch_bwamem2_bam = Channel.empty() + ch_sentieon_bam = Channel.empty() + ch_bwamem2_bai = Channel.empty() + ch_sentieon_bai = Channel.empty() - ALIGN_BWAMEM2 ( // Triggered when params.aligner is set as bwamem2 - ch_reads, - ch_genome_bwamem2index, - ch_genome_fasta, - ch_genome_fai, - val_platform - ) - - ALIGN_SENTIEON ( // Triggered when params.aligner is set as sentieon - ch_reads, - ch_genome_fasta, - ch_genome_fai, - ch_genome_bwaindex, - val_platform - ) + if (params.aligner.equals("bwamem2")) { + ALIGN_BWAMEM2 ( // Triggered when params.aligner is set as bwamem2 + ch_reads, + ch_genome_bwamem2index, + ch_genome_fasta, + ch_genome_fai, + val_platform + ) + ch_bwamem2_bam = ALIGN_BWAMEM2.out.marked_bam + ch_bwamem2_bai = ALIGN_BWAMEM2.out.marked_bai + ch_versions = ch_versions.mix(ALIGN_BWAMEM2.out.versions) + } else if (params.aligner.equals("sentieon")) { + ALIGN_SENTIEON ( // Triggered when params.aligner is set as sentieon + ch_reads, + ch_genome_fasta, + ch_genome_fai, + ch_genome_bwaindex, + val_platform + ) + ch_sentieon_bam = ALIGN_SENTIEON.out.marked_bam + ch_sentieon_bai = ALIGN_SENTIEON.out.marked_bai + ch_versions = ch_versions.mix(ALIGN_SENTIEON.out.versions) + } - ch_genome_marked_bam = Channel.empty().mix(ALIGN_BWAMEM2.out.marked_bam, ALIGN_SENTIEON.out.marked_bam) - ch_genome_marked_bai = Channel.empty().mix(ALIGN_BWAMEM2.out.marked_bai, ALIGN_SENTIEON.out.marked_bai) + ch_genome_marked_bam = Channel.empty().mix(ch_bwamem2_bam, ch_sentieon_bam) + ch_genome_marked_bai = Channel.empty().mix(ch_bwamem2_bai, ch_sentieon_bai) ch_genome_bam_bai = ch_genome_marked_bam.join(ch_genome_marked_bai, failOnMismatch:true, failOnDuplicate:true) // PREPARING READS FOR MT ALIGNMENT @@ -83,14 +95,13 @@ workflow ALIGN { ch_mtshift_marked_bai = ALIGN_MT_SHIFT.out.marked_bai ch_mtshift_bam_bai = ch_mtshift_marked_bam.join(ch_mtshift_marked_bai, failOnMismatch:true, failOnDuplicate:true) - SAMTOOLS_VIEW( ch_genome_bam_bai, ch_genome_fasta, [] ) - - ch_versions = Channel.empty().mix(ALIGN_BWAMEM2.out.versions, - ALIGN_SENTIEON.out.versions, - ALIGN_MT.out.versions, - ALIGN_MT_SHIFT.out.versions, - CONVERT_MT_BAM_TO_FASTQ.out.versions, - SAMTOOLS_VIEW.out.versions) + if (params.save_mapped_as_cram) { + SAMTOOLS_VIEW( ch_genome_bam_bai, ch_genome_fasta, [] ) + ch_versions = ch_versions.mix(SAMTOOLS_VIEW.out.versions) + } + ch_versions = ch_versions.mix(ALIGN_MT.out.versions, + ALIGN_MT_SHIFT.out.versions, + CONVERT_MT_BAM_TO_FASTQ.out.versions) emit: genome_marked_bam = ch_genome_marked_bam // channel: [ val(meta), path(bam) ] diff --git a/subworkflows/local/alignment/align_MT.nf b/subworkflows/local/alignment/align_MT.nf index dec73e67..11c51c6d 100644 --- a/subworkflows/local/alignment/align_MT.nf +++ b/subworkflows/local/alignment/align_MT.nf @@ -21,14 +21,22 @@ workflow ALIGN_MT { ch_fai // channel: [mandatory] [ val(meta), path(fai) ] main: - ch_versions = Channel.empty() - - BWAMEM2_MEM_MT (ch_fastq, ch_bwamem2index, true) - - SENTIEON_BWAMEM_MT ( ch_fastq, ch_bwaindex, ch_fasta, ch_fai ) + ch_versions = Channel.empty() + ch_bwamem2_bam = Channel.empty() + ch_sentieon_bam = Channel.empty() + + if (params.aligner.equals("bwamem2")) { + BWAMEM2_MEM_MT (ch_fastq, ch_bwamem2index, true) + ch_bwamem2_bam = BWAMEM2_MEM_MT.out.bam + ch_versions = ch_versions.mix(BWAMEM2_MEM_MT.out.versions.first()) + } else if (params.aligner.equals("sentieon")) { + SENTIEON_BWAMEM_MT ( ch_fastq, ch_bwaindex, ch_fasta, ch_fai ) + ch_sentieon_bam = SENTIEON_BWAMEM_MT.out.bam_and_bai.map{ meta, bam, bai -> [meta, bam] } + ch_versions = ch_versions.mix(BWAMEM2_MEM_MT.out.versions.first()) + } Channel.empty() - .mix(BWAMEM2_MEM_MT.out.bam, SENTIEON_BWAMEM_MT.out.bam_and_bai.map{ meta, bam, bai -> [meta, bam] }) + .mix(ch_bwamem2_bam, ch_sentieon_bam) .join(ch_ubam, failOnMismatch:true, failOnDuplicate:true) .set {ch_bam_ubam} @@ -42,8 +50,6 @@ workflow ALIGN_MT { SAMTOOLS_INDEX_MT(SAMTOOLS_SORT_MT.out.bam) - ch_versions = ch_versions.mix(BWAMEM2_MEM_MT.out.versions.first()) - ch_versions = ch_versions.mix(SENTIEON_BWAMEM_MT.out.versions.first()) ch_versions = ch_versions.mix(GATK4_MERGEBAMALIGNMENT_MT.out.versions.first()) ch_versions = ch_versions.mix(PICARD_ADDORREPLACEREADGROUPS_MT.out.versions.first()) ch_versions = ch_versions.mix(PICARD_MARKDUPLICATES_MT.out.versions.first()) diff --git a/subworkflows/local/annotate_genome_snvs.nf b/subworkflows/local/annotate_genome_snvs.nf index fe38746b..334b7d4b 100644 --- a/subworkflows/local/annotate_genome_snvs.nf +++ b/subworkflows/local/annotate_genome_snvs.nf @@ -38,6 +38,7 @@ workflow ANNOTATE_GENOME_SNVS { ch_split_intervals // channel: [mandatory] [ path(intervals) ] main: + ch_cadd_vcf = Channel.empty() ch_versions = Channel.empty() ch_vcf_scatter_in = Channel.empty() ch_vep_in = Channel.empty() @@ -92,16 +93,20 @@ workflow ANNOTATE_GENOME_SNVS { GATK4_SELECTVARIANTS (ch_vcf_scatter_in) // Annotating with CADD - ANNOTATE_CADD ( - GATK4_SELECTVARIANTS.out.vcf, - GATK4_SELECTVARIANTS.out.tbi, - ch_cadd_header, - ch_cadd_resources - ) + if (params.cadd_resources != null) { + ANNOTATE_CADD ( + GATK4_SELECTVARIANTS.out.vcf, + GATK4_SELECTVARIANTS.out.tbi, + ch_cadd_header, + ch_cadd_resources + ) + ch_cadd_vcf = ANNOTATE_CADD.out.vcf + ch_versions = ch_versions.mix(ANNOTATE_CADD.out.versions) + } // If CADD is run, pick CADD output as input for VEP else pass selectvariants output to VEP. GATK4_SELECTVARIANTS.out.vcf - .join(ANNOTATE_CADD.out.vcf, remainder: true) // If CADD is not run then the third element in this channel will be `null` + .join(ch_cadd_vcf, remainder: true) // If CADD is not run then the third element in this channel will be `null` .branch { it -> // If CADD is run, then "it" will be [[meta],selvar.vcf,cadd.vcf], else [[meta],selvar.vcf,null] selvar: it[2].equals(null) return [it[0], it[1]] @@ -158,7 +163,6 @@ workflow ANNOTATE_GENOME_SNVS { ch_versions = ch_versions.mix(BCFTOOLS_VIEW.out.versions) ch_versions = ch_versions.mix(TABIX_BCFTOOLS_VIEW.out.versions) ch_versions = ch_versions.mix(GATK4_SELECTVARIANTS.out.versions.first()) - ch_versions = ch_versions.mix(ANNOTATE_CADD.out.versions) ch_versions = ch_versions.mix(ENSEMBLVEP_SNV.out.versions.first()) ch_versions = ch_versions.mix(TABIX_VEP.out.versions.first()) ch_versions = ch_versions.mix(BCFTOOLS_CONCAT.out.versions) diff --git a/subworkflows/local/annotate_mt_snvs.nf b/subworkflows/local/annotate_mt_snvs.nf index 8f449e7b..8f7c24eb 100644 --- a/subworkflows/local/annotate_mt_snvs.nf +++ b/subworkflows/local/annotate_mt_snvs.nf @@ -24,20 +24,25 @@ workflow ANNOTATE_MT_SNVS { ch_vep_cache // channel: [mandatory] [ path(cache) ] main: + ch_cadd_vcf = Channel.empty() ch_versions = Channel.empty() // Annotating with CADD - ANNOTATE_CADD ( - ch_mt_vcf, - ch_mt_tbi, - ch_cadd_header, - ch_cadd_resources - ) + if (params.cadd_resources != null) { + ANNOTATE_CADD ( + ch_mt_vcf, + ch_mt_tbi, + ch_cadd_header, + ch_cadd_resources + ) + ch_cadd_vcf = ANNOTATE_CADD.out.vcf + ch_versions = ch_versions.mix(ANNOTATE_CADD.out.versions) + } // Pick input for vep ch_mt_vcf - .join(ANNOTATE_CADD.out.vcf, remainder: true) // If CADD is not run then the third element in this channel will be `null` - .branch { it -> // If CADD is run, then "it" will be [[meta],selvar.vcf,cadd.vcf], else [[meta],selvar.vcf,null] + .join(ch_cadd_vcf, remainder: true) // If CADD is not run then the third element in this channel will be `null` + .branch { it -> // If CADD is run, then "it" will be [[meta],selvar.vcf,cadd.vcf], else [[meta],selvar.vcf,null] merged: it[2].equals(null) return [it[0], it[1]] cadd: !(it[2].equals(null)) @@ -81,7 +86,6 @@ workflow ANNOTATE_MT_SNVS { // Running haplogrep2 HAPLOGREP2_CLASSIFY_MT(ch_vep_in, "vcf.gz") - ch_versions = ch_versions.mix(ANNOTATE_CADD.out.versions) ch_versions = ch_versions.mix(ENSEMBLVEP_MT.out.versions) ch_versions = ch_versions.mix(TABIX_TABIX_MT.out.versions) ch_versions = ch_versions.mix(VCFANNO_MT.out.versions) diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index 68ec15a2..d363d35c 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -31,31 +31,41 @@ workflow CALL_SNV { ch_pcr_indel_model // channel: [optional] [ val(sentieon_dnascope_pcr_indel_model) ] main: - ch_versions = Channel.empty() - ch_vcf = Channel.empty() - ch_tabix = Channel.empty() + ch_versions = Channel.empty() + ch_deepvar_vcf = Channel.empty() + ch_deepvar_tbi = Channel.empty() + ch_sentieon_vcf = Channel.empty() + ch_sentieon_tbi = Channel.empty() - CALL_SNV_DEEPVARIANT ( // triggered only when params.variant_caller is set as deepvariant - ch_genome_bam_bai, - ch_genome_fasta, - ch_genome_fai, - ch_case_info - ) - - CALL_SNV_SENTIEON( // triggered only when params.variant_caller is set as sentieon - ch_genome_bam_bai, - ch_genome_fasta, - ch_genome_fai, - ch_dbsnp, - ch_dbsnp_tbi, - ch_call_interval, - ch_ml_model, - ch_case_info, - ch_pcr_indel_model - ) + if (params.variant_caller.equals("deepvariant")) { + CALL_SNV_DEEPVARIANT ( // triggered only when params.variant_caller is set as deepvariant + ch_genome_bam_bai, + ch_genome_fasta, + ch_genome_fai, + ch_case_info + ) + ch_deepvar_vcf = CALL_SNV_DEEPVARIANT.out.vcf + ch_deepvar_tbi = CALL_SNV_DEEPVARIANT.out.tabix + ch_versions = ch_versions.mix(CALL_SNV_DEEPVARIANT.out.versions) + } else if (params.variant_caller.equals("sentieon")) { + CALL_SNV_SENTIEON( // triggered only when params.variant_caller is set as sentieon + ch_genome_bam_bai, + ch_genome_fasta, + ch_genome_fai, + ch_dbsnp, + ch_dbsnp_tbi, + ch_call_interval, + ch_ml_model, + ch_case_info, + ch_pcr_indel_model + ) + ch_sentieon_vcf = CALL_SNV_SENTIEON.out.vcf + ch_sentieon_tbi = CALL_SNV_SENTIEON.out.tabix + ch_versions = ch_versions.mix(CALL_SNV_DEEPVARIANT.out.versions) + } - ch_vcf = Channel.empty().mix(CALL_SNV_DEEPVARIANT.out.vcf, CALL_SNV_SENTIEON.out.vcf) - ch_tabix = Channel.empty().mix(CALL_SNV_DEEPVARIANT.out.tabix, CALL_SNV_SENTIEON.out.tabix) + ch_vcf = Channel.empty().mix(ch_deepvar_vcf, ch_sentieon_vcf) + ch_tabix = Channel.empty().mix(ch_deepvar_tbi, ch_sentieon_tbi) ch_vcf .join(ch_tabix, failOnMismatch:true, failOnDuplicate:true) @@ -93,8 +103,6 @@ workflow CALL_SNV { ch_case_info ) - ch_versions = ch_versions.mix(CALL_SNV_DEEPVARIANT.out.versions) - ch_versions = ch_versions.mix(CALL_SNV_SENTIEON.out.versions) ch_versions = ch_versions.mix(CALL_SNV_MT.out.versions) ch_versions = ch_versions.mix(CALL_SNV_MT_SHIFT.out.versions) ch_versions = ch_versions.mix(POSTPROCESS_MT_CALLS.out.versions) diff --git a/subworkflows/local/call_structural_variants.nf b/subworkflows/local/call_structural_variants.nf index 28f4e8db..961f02a4 100644 --- a/subworkflows/local/call_structural_variants.nf +++ b/subworkflows/local/call_structural_variants.nf @@ -43,15 +43,19 @@ workflow CALL_STRUCTURAL_VARIANTS { .collect{it[1]} .set { tiddit_vcf } - CALL_SV_GERMLINECNVCALLER (ch_genome_bam_bai, ch_genome_fasta, ch_genome_fai, ch_readcount_intervals, ch_genome_dictionary, ch_ploidy_model, ch_gcnvcaller_model) - .genotyped_intervals_vcf - .collect{it[1]} - .set { gcnvcaller_vcf } + if (!params.skip_germlinecnvcaller) { + CALL_SV_GERMLINECNVCALLER (ch_genome_bam_bai, ch_genome_fasta, ch_genome_fai, ch_readcount_intervals, ch_genome_dictionary, ch_ploidy_model, ch_gcnvcaller_model) + .genotyped_intervals_vcf + .collect{it[1]} + .set { gcnvcaller_vcf } + + ch_versions = ch_versions.mix(CALL_SV_GERMLINECNVCALLER.out.versions) + } CALL_SV_MT (ch_mt_bam_bai, ch_genome_fasta) //merge - if (params.skip_cnv_calling) { + if (params.skip_germlinecnvcaller) { tiddit_vcf .combine(manta_vcf) .toList() @@ -75,7 +79,6 @@ workflow CALL_STRUCTURAL_VARIANTS { ch_versions = ch_versions.mix(CALL_SV_MANTA.out.versions) ch_versions = ch_versions.mix(CALL_SV_MT.out.versions) ch_versions = ch_versions.mix(CALL_SV_TIDDIT.out.versions) - ch_versions = ch_versions.mix(CALL_SV_GERMLINECNVCALLER.out.versions) ch_versions = ch_versions.mix(TABIX_TABIX.out.versions) emit: diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 95d95590..5308b414 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -54,7 +54,7 @@ if (params.variant_caller.equals("sentieon")) { mandatoryParams += ["ml_model"] } -if (!params.skip_cnv_calling) { +if (!params.skip_germlinecnvcaller) { mandatoryParams += ["ploidy_model", "gcnvcaller_model"] } @@ -277,7 +277,7 @@ workflow RAREDISEASE { // SV caller priority - if (params.skip_cnv_calling) { + if (params.skip_germlinecnvcaller) { ch_svcaller_priority = Channel.value(["tiddit", "manta"]) } else { ch_svcaller_priority = Channel.value(["tiddit", "manta", "gcnvcaller"]) From 3a16d93f0fd4249678761475ffb22302afea0d76 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 17 Oct 2023 16:56:08 +0200 Subject: [PATCH 135/387] add skip_qualimap --- conf/modules/qc_bam.config | 6 ++++-- conf/test.config | 1 + conf/test_one_sample.config | 1 + nextflow.config | 1 + nextflow_schema.json | 5 +++++ subworkflows/local/qc_bam.nf | 9 ++++++--- 6 files changed, 18 insertions(+), 5 deletions(-) diff --git a/conf/modules/qc_bam.config b/conf/modules/qc_bam.config index eeb60b34..fe2031a9 100644 --- a/conf/modules/qc_bam.config +++ b/conf/modules/qc_bam.config @@ -33,8 +33,10 @@ process { ext.prefix = { "${meta.id}_hsmetrics" } } - withName: '.*QC_BAM:QUALIMAP_BAMQC' { - ext.prefix = { "${meta.id}_qualimap" } + if (params.skip_qualimap) { + withName: '.*QC_BAM:QUALIMAP_BAMQC' { + ext.prefix = { "${meta.id}_qualimap" } + } } withName: '.*QC_BAM:TIDDIT_COV' { diff --git a/conf/test.config b/conf/test.config index c7593f76..04c41e14 100644 --- a/conf/test.config +++ b/conf/test.config @@ -25,6 +25,7 @@ params { // analysis params skip_cnv_calling = true + skip_qualimap = true skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI // Input data diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index 51524fe7..0a017791 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -25,6 +25,7 @@ params { // analysis params skip_cnv_calling = true + skip_qualimap = true skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI // Input data diff --git a/nextflow.config b/nextflow.config index 9573e35c..348a92a6 100644 --- a/nextflow.config +++ b/nextflow.config @@ -25,6 +25,7 @@ params { bait_padding = 100 save_mapped_as_cram = false skip_cnv_calling = false + skip_qualimap = false skip_snv_annotation = false skip_sv_annotation = false skip_mt_annotation = false diff --git a/nextflow_schema.json b/nextflow_schema.json index a3a95f1c..9c336f06 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -388,6 +388,11 @@ "description": "Specifies whether or not to skip CNV calling.", "fa_icon": "fas fa-toggle-on" }, + "skip_qualimap": { + "type": "boolean", + "description": "Specifies whether or not to skip Qualimap.", + "fa_icon": "fas fa-toggle-on" + }, "skip_mt_annotation": { "type": "boolean", "description": "Specifies whether or not to skip annotation of mitochondrial variants.", diff --git a/subworkflows/local/qc_bam.nf b/subworkflows/local/qc_bam.nf index 9331523e..11439a7b 100644 --- a/subworkflows/local/qc_bam.nf +++ b/subworkflows/local/qc_bam.nf @@ -29,6 +29,7 @@ workflow QC_BAM { main: ch_versions = Channel.empty() + ch_qualimap = Channel.empty() PICARD_COLLECTMULTIPLEMETRICS (ch_bam_bai, ch_genome_fasta, ch_genome_fai) @@ -39,7 +40,10 @@ workflow QC_BAM { PICARD_COLLECTHSMETRICS (ch_hsmetrics_in, ch_genome_fasta, ch_genome_fai, [[],[]]) - QUALIMAP_BAMQC (ch_bam, []) + if (!params.skip_qualimap) { + ch_qualimap = QUALIMAP_BAMQC (ch_bam, []).results + ch_versions = ch_versions.mix(QUALIMAP_BAMQC.out.versions.first()) + } TIDDIT_COV (ch_bam, [[],[]]) // 2nd pos. arg is req. only for cram input @@ -60,7 +64,6 @@ workflow QC_BAM { ch_versions = ch_versions.mix(PICARD_COLLECTMULTIPLEMETRICS.out.versions.first()) ch_versions = ch_versions.mix(PICARD_COLLECTHSMETRICS.out.versions.first()) - ch_versions = ch_versions.mix(QUALIMAP_BAMQC.out.versions.first()) ch_versions = ch_versions.mix(TIDDIT_COV.out.versions.first()) ch_versions = ch_versions.mix(UCSC_WIGTOBIGWIG.out.versions.first()) ch_versions = ch_versions.mix(MOSDEPTH.out.versions.first()) @@ -70,7 +73,7 @@ workflow QC_BAM { emit: multiple_metrics = PICARD_COLLECTMULTIPLEMETRICS.out.metrics // channel: [ val(meta), path(metrics) ] hs_metrics = PICARD_COLLECTHSMETRICS.out.metrics // channel: [ val(meta), path(metrics) ] - qualimap_results = QUALIMAP_BAMQC.out.results // channel: [ val(meta), path(qualimap_dir) ] + qualimap_results = ch_qualimap // channel: [ val(meta), path(qualimap_dir) ] tiddit_wig = TIDDIT_COV.out.wig // channel: [ val(meta), path(wig) ] bigwig = UCSC_WIGTOBIGWIG.out.bw // channel: [ val(meta), path(bw) ] d4 = MOSDEPTH.out.per_base_d4 // channel: [ val(meta), path(d4) ] From 164a8f7a1e6587938109d91f7e9271030600dc6b Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 17 Oct 2023 16:58:34 +0200 Subject: [PATCH 136/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 2def1d5b..b05be855 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -11,6 +11,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - A new parameter to skip filtering based on vep results [#416](https://github.com/nf-core/raredisease/pull/416) - A `metromap` representating the core parts of the pipeline [#428](https://github.com/nf-core/raredisease/pull/428) - Metromap and logos for light and dark theme [#432](https://github.com/nf-core/raredisease/pull/432) +- A new parameter to skip qualimap [#436](https://github.com/nf-core/raredisease/pull/436) ### `Changed` From 24e8deb359f8885c848be0efa3c0e2006abe7473 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 17 Oct 2023 17:24:25 +0200 Subject: [PATCH 137/387] skip eklipse --- CHANGELOG.md | 2 +- conf/modules/call_sv_MT.config | 14 ++++++++------ conf/test.config | 1 + conf/test_one_sample.config | 1 + nextflow.config | 1 + nextflow_schema.json | 5 +++++ .../local/variant_calling/call_sv_MT.nf | 17 +++++++++++------ 7 files changed, 28 insertions(+), 13 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index b05be855..1623fc11 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -11,7 +11,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - A new parameter to skip filtering based on vep results [#416](https://github.com/nf-core/raredisease/pull/416) - A `metromap` representating the core parts of the pipeline [#428](https://github.com/nf-core/raredisease/pull/428) - Metromap and logos for light and dark theme [#432](https://github.com/nf-core/raredisease/pull/432) -- A new parameter to skip qualimap [#436](https://github.com/nf-core/raredisease/pull/436) +- New parameters to skip qualimap and eklipse (`--skip_qualimap` and `--skip_eklipse`) [#436](https://github.com/nf-core/raredisease/pull/436) ### `Changed` diff --git a/conf/modules/call_sv_MT.config b/conf/modules/call_sv_MT.config index 44b0e581..606ef429 100644 --- a/conf/modules/call_sv_MT.config +++ b/conf/modules/call_sv_MT.config @@ -26,12 +26,14 @@ process { ] } - withName: '.*CALL_SV_MT:EKLIPSE' { - publishDir = [ - path: { "${params.outdir}/call_sv/mitochondria" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + if (!params.skip_eklipse){ + withName: '.*CALL_SV_MT:EKLIPSE' { + publishDir = [ + path: { "${params.outdir}/call_sv/mitochondria" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } } diff --git a/conf/test.config b/conf/test.config index 04c41e14..8cb3e2af 100644 --- a/conf/test.config +++ b/conf/test.config @@ -25,6 +25,7 @@ params { // analysis params skip_cnv_calling = true + skip_eklipse = true skip_qualimap = true skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index 0a017791..4ad89508 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -25,6 +25,7 @@ params { // analysis params skip_cnv_calling = true + skip_eklipse = true skip_qualimap = true skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI diff --git a/nextflow.config b/nextflow.config index 348a92a6..ed8275fe 100644 --- a/nextflow.config +++ b/nextflow.config @@ -25,6 +25,7 @@ params { bait_padding = 100 save_mapped_as_cram = false skip_cnv_calling = false + skip_eklipse = false skip_qualimap = false skip_snv_annotation = false skip_sv_annotation = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 9c336f06..6d43a432 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -388,6 +388,11 @@ "description": "Specifies whether or not to skip CNV calling.", "fa_icon": "fas fa-toggle-on" }, + "skip_eklipse": { + "type": "boolean", + "description": "Specifies whether or not to skip eKLIPse.", + "fa_icon": "fas fa-toggle-on" + }, "skip_qualimap": { "type": "boolean", "description": "Specifies whether or not to skip Qualimap.", diff --git a/subworkflows/local/variant_calling/call_sv_MT.nf b/subworkflows/local/variant_calling/call_sv_MT.nf index c8fdff45..41b91119 100644 --- a/subworkflows/local/variant_calling/call_sv_MT.nf +++ b/subworkflows/local/variant_calling/call_sv_MT.nf @@ -11,19 +11,24 @@ workflow CALL_SV_MT { ch_fasta // channel: [mandatory] [ val(meta), path(fasta) ] main: - ch_versions = Channel.empty() + ch_versions = Channel.empty() + ch_eklipse_del = Channel.empty() + ch_eklipse_genes = Channel.empty() + ch_eklipse_circos = Channel.empty() - EKLIPSE(ch_bam_bai,[]) + if (!params.skip_eklipse){ + EKLIPSE(ch_bam_bai,[]) + ch_versions = ch_versions.mix(EKLIPSE.out.versions.first()) + } MT_DELETION(ch_bam_bai, ch_fasta) - ch_versions = ch_versions.mix(EKLIPSE.out.versions.first()) ch_versions = ch_versions.mix(MT_DELETION.out.versions.first()) emit: - eklipse_del = EKLIPSE.out.deletions // channel: [ val(meta), path(csv) ] - eklipse_genes = EKLIPSE.out.genes // channel: [ val(meta), path(csv) ] - eklipse_circos = EKLIPSE.out.circos // channel: [ val(meta), path(png) ] + eklipse_del = ch_eklipse_del // channel: [ val(meta), path(csv) ] + eklipse_genes = ch_eklipse_genes // channel: [ val(meta), path(csv) ] + eklipse_circos = ch_eklipse_circos // channel: [ val(meta), path(png) ] mt_del_result = MT_DELETION.out.mt_del_result // channel: [ val(meta), path(txt) ] versions = ch_versions // channel: [ path(versions.yml) ] } From bcbdf5d58898634d7813c81c90d53578a8a8d269 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 17 Oct 2023 18:02:50 +0200 Subject: [PATCH 138/387] update changelog --- CHANGELOG.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 1623fc11..3d64c97a 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -12,6 +12,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - A `metromap` representating the core parts of the pipeline [#428](https://github.com/nf-core/raredisease/pull/428) - Metromap and logos for light and dark theme [#432](https://github.com/nf-core/raredisease/pull/432) - New parameters to skip qualimap and eklipse (`--skip_qualimap` and `--skip_eklipse`) [#436](https://github.com/nf-core/raredisease/pull/436) +- Fix "there is no process matching config selector warnings" [#435](https://github.com/nf-core/raredisease/pull/435) ### `Changed` @@ -28,6 +29,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Breaks down mitochondrial analysis workflow into smaller subworkflows that are more modular [#419](https://github.com/nf-core/raredisease/pull/419) - Replaced the parameter skip_mt_analysis which was used to turn on/off the mitochondrial workflow [#419](https://github.com/nf-core/raredisease/pull/419) - Adds a new parameter skip_mt_annotation which can be used to turn on/off annotation and ranking for mitochondrial SNVs [#419](https://github.com/nf-core/raredisease/pull/419) +- Changed the name of the parameter from `skip_cnv_calling` to `skip_germlinecnvcaller` [#435](https://github.com/nf-core/raredisease/pull/435) ### `Fixed` From 9b1b6c6c7f581324f87f4cc219dd381c2593c744 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 18 Oct 2023 12:37:31 +0200 Subject: [PATCH 139/387] review comments --- conf/modules/qc_bam.config | 2 +- subworkflows/local/variant_calling/call_sv_MT.nf | 3 +++ 2 files changed, 4 insertions(+), 1 deletion(-) diff --git a/conf/modules/qc_bam.config b/conf/modules/qc_bam.config index fe2031a9..cc744773 100644 --- a/conf/modules/qc_bam.config +++ b/conf/modules/qc_bam.config @@ -33,7 +33,7 @@ process { ext.prefix = { "${meta.id}_hsmetrics" } } - if (params.skip_qualimap) { + if (!params.skip_qualimap) { withName: '.*QC_BAM:QUALIMAP_BAMQC' { ext.prefix = { "${meta.id}_qualimap" } } diff --git a/subworkflows/local/variant_calling/call_sv_MT.nf b/subworkflows/local/variant_calling/call_sv_MT.nf index 41b91119..7d6f4ede 100644 --- a/subworkflows/local/variant_calling/call_sv_MT.nf +++ b/subworkflows/local/variant_calling/call_sv_MT.nf @@ -18,6 +18,9 @@ workflow CALL_SV_MT { if (!params.skip_eklipse){ EKLIPSE(ch_bam_bai,[]) + ch_eklipse_del = EKLIPSE.out.deletions + ch_eklipse_genes = EKLIPSE.out.genes + ch_eklipse_circos = EKLIPSE.out.circos ch_versions = ch_versions.mix(EKLIPSE.out.versions.first()) } From 181cfa68ef34a2f6e739b8986406a2a77f02aca3 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 26 Oct 2023 08:36:54 +0200 Subject: [PATCH 140/387] update test config --- conf/test.config | 6 +++--- conf/test_one_sample.config | 6 +++--- 2 files changed, 6 insertions(+), 6 deletions(-) diff --git a/conf/test.config b/conf/test.config index 64848661..64f5fdca 100644 --- a/conf/test.config +++ b/conf/test.config @@ -24,9 +24,9 @@ params { mito_name = 'MT' // analysis params - skip_eklipse = true - skip_germlinecnvcaller = true - skip_qualimap = true + skip_eklipse = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_germlinecnvcaller = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_qualimap = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI // Input data diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index 62eaac45..67ea9f47 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -24,9 +24,9 @@ params { mito_name = 'MT' // analysis params - skip_eklipse = true - skip_germlinecnvcaller = true - skip_qualimap = true + skip_eklipse = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_germlinecnvcaller = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_qualimap = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI // Input data From bc1f6c8092ff044e7bae98bdae1216d95b1dbf24 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 26 Oct 2023 14:10:32 +0200 Subject: [PATCH 141/387] skip haplocheck and fastqc [skip ci] --- conf/modules/raredisease.config | 15 +++++++++------ conf/test.config | 2 ++ conf/test_one_sample.config | 2 ++ nextflow.config | 2 ++ nextflow_schema.json | 10 ++++++++++ .../local/variant_calling/call_snv_MT.nf | 16 +++++++++++----- workflows/raredisease.nf | 11 +++++++---- 7 files changed, 43 insertions(+), 15 deletions(-) diff --git a/conf/modules/raredisease.config b/conf/modules/raredisease.config index 154daa03..c24267d6 100644 --- a/conf/modules/raredisease.config +++ b/conf/modules/raredisease.config @@ -31,12 +31,15 @@ process { // process { - withName: '.*RAREDISEASE:FASTQC' { - ext.args = '--quiet' - publishDir = [ - path: { "${params.outdir}/fastqc/${meta.id}" }, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, - ] + + if (!params.skip_fastqc) { + withName: '.*RAREDISEASE:FASTQC' { + ext.args = '--quiet' + publishDir = [ + path: { "${params.outdir}/fastqc/${meta.id}" }, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + ] + } } } diff --git a/conf/test.config b/conf/test.config index 64848661..06ccf862 100644 --- a/conf/test.config +++ b/conf/test.config @@ -25,7 +25,9 @@ params { // analysis params skip_eklipse = true + skip_fastqc = true skip_germlinecnvcaller = true + skip_haplocheck = true skip_qualimap = true skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index 62eaac45..97441f99 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -25,7 +25,9 @@ params { // analysis params skip_eklipse = true + skip_fastqc = true skip_germlinecnvcaller = true + skip_haplocheck = true skip_qualimap = true skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI diff --git a/nextflow.config b/nextflow.config index 6bea3b50..775d5a81 100644 --- a/nextflow.config +++ b/nextflow.config @@ -25,7 +25,9 @@ params { bait_padding = 100 save_mapped_as_cram = false skip_eklipse = false + skip_fastqc = false skip_germlinecnvcaller = false + skip_haplocheck = false skip_qualimap = false skip_snv_annotation = false skip_sv_annotation = false diff --git a/nextflow_schema.json b/nextflow_schema.json index eda0202c..496baaf6 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -383,6 +383,16 @@ "description": "Specifies whether to generate and publish alignment files as cram instead of bam", "fa_icon": "fas fa-toggle-on" }, + "skip_fastqc": { + "type": "boolean", + "description": "Specifies whether or not to skip FASTQC.", + "fa_icon": "fas fa-toggle-on" + }, + "skip_haplocheck": { + "type": "boolean", + "description": "Specifies whether or not to skip haplocheck.", + "fa_icon": "fas fa-toggle-on" + }, "skip_germlinecnvcaller": { "type": "boolean", "description": "Specifies whether or not to skip CNV calling.", diff --git a/subworkflows/local/variant_calling/call_snv_MT.nf b/subworkflows/local/variant_calling/call_snv_MT.nf index d55d1965..b0a2fbae 100644 --- a/subworkflows/local/variant_calling/call_snv_MT.nf +++ b/subworkflows/local/variant_calling/call_snv_MT.nf @@ -15,13 +15,20 @@ workflow CALL_SNV_MT { ch_intervals // channel: [mandatory] [ path(interval_list) ] main: - ch_versions = Channel.empty() + ch_versions = Channel.empty() + ch_haplocheck_txt = Channel.empty() + ch_haplocheck_html = Channel.empty() ch_bam_bai_int = ch_bam_bai.combine(ch_intervals) GATK4_MUTECT2_MT (ch_bam_bai_int, ch_fasta, ch_fai, ch_dict, [], [], [],[]) - HAPLOCHECK_MT (GATK4_MUTECT2_MT.out.vcf) + if (!params.skip_haplocheck) { + HAPLOCHECK_MT (GATK4_MUTECT2_MT.out.vcf).set { ch_haplocheck } + ch_versions = ch_versions.mix(HAPLOCHECK_MT.out.versions.first()) + ch_haplocheck_txt = HAPLOCHECK_MT.out.txt + ch_haplocheck_html = HAPLOCHECK_MT.out.html + } // Filter Mutect2 calls ch_mutect_vcf = GATK4_MUTECT2_MT.out.vcf.join(GATK4_MUTECT2_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) @@ -34,7 +41,6 @@ workflow CALL_SNV_MT { GATK4_FILTERMUTECTCALLS_MT (ch_to_filt, ch_fasta, ch_fai, ch_dict) ch_versions = ch_versions.mix(GATK4_MUTECT2_MT.out.versions.first()) - ch_versions = ch_versions.mix(HAPLOCHECK_MT.out.versions.first()) ch_versions = ch_versions.mix(GATK4_FILTERMUTECTCALLS_MT.out.versions.first()) emit: @@ -42,7 +48,7 @@ workflow CALL_SNV_MT { tbi = GATK4_FILTERMUTECTCALLS_MT.out.tbi // channel: [ val(meta), path(tbi) ] stats = GATK4_MUTECT2_MT.out.stats // channel: [ val(meta), path(stats) ] filt_stats = GATK4_FILTERMUTECTCALLS_MT.out.stats // channel: [ val(meta), path(tsv) ] - txt = HAPLOCHECK_MT.out.txt // channel: [ val(meta), path(txt) ] - html = HAPLOCHECK_MT.out.html // channel: [ val(meta), path(html) ] + txt = ch_haplocheck_txt // channel: [ val(meta), path(txt) ] + html = ch_haplocheck_html // channel: [ val(meta), path(html) ] versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 5308b414..5a164a39 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -284,9 +284,10 @@ workflow RAREDISEASE { } // Input QC - FASTQC (ch_reads) - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - + if (!params.skip_fastqc) { + FASTQC (ch_reads) + ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + } // CREATE CHROMOSOME BED AND INTERVALS SCATTER_GENOME ( ch_genome_dictionary, @@ -593,7 +594,9 @@ workflow RAREDISEASE { ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + if (!params.skip_fastqc) { + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + } ch_multiqc_files = ch_multiqc_files.mix(QC_BAM.out.multiple_metrics.map{it[1]}.collect().ifEmpty([])) ch_multiqc_files = ch_multiqc_files.mix(QC_BAM.out.hs_metrics.map{it[1]}.collect().ifEmpty([])) ch_multiqc_files = ch_multiqc_files.mix(QC_BAM.out.qualimap_results.map{it[1]}.collect().ifEmpty([])) From 8480d37e427caf2b0f9d4c5839f73c5a442c4d80 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 26 Oct 2023 14:15:38 +0200 Subject: [PATCH 142/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 3d64c97a..f4d17c8e 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -13,6 +13,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Metromap and logos for light and dark theme [#432](https://github.com/nf-core/raredisease/pull/432) - New parameters to skip qualimap and eklipse (`--skip_qualimap` and `--skip_eklipse`) [#436](https://github.com/nf-core/raredisease/pull/436) - Fix "there is no process matching config selector warnings" [#435](https://github.com/nf-core/raredisease/pull/435) +- New parameters to skip fastqc and haplocheck (`--skip_fastqc` and `--skip_haplocheck`) [#438](https://github.com/nf-core/raredisease/pull/438) ### `Changed` From c34e516cf8e9640c4f1636913ec2770ab6576ab3 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 26 Oct 2023 19:37:05 +0530 Subject: [PATCH 143/387] Update test_one_sample.config [skip ci] --- conf/test_one_sample.config | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index 97441f99..f39cb40c 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -24,11 +24,11 @@ params { mito_name = 'MT' // analysis params - skip_eklipse = true - skip_fastqc = true - skip_germlinecnvcaller = true - skip_haplocheck = true - skip_qualimap = true + skip_eklipse = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_fastqc = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_germlinecnvcaller = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_haplocheck = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_qualimap = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI // Input data From a88a83b9c8ef08daf8f25670c7b03470b4b816a7 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 26 Oct 2023 19:37:59 +0530 Subject: [PATCH 144/387] Update test.config --- conf/test.config | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/conf/test.config b/conf/test.config index 06ccf862..d398d542 100644 --- a/conf/test.config +++ b/conf/test.config @@ -24,11 +24,11 @@ params { mito_name = 'MT' // analysis params - skip_eklipse = true - skip_fastqc = true - skip_germlinecnvcaller = true - skip_haplocheck = true - skip_qualimap = true + skip_eklipse = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_fastqc = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_germlinecnvcaller = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_haplocheck = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_qualimap = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI // Input data From bdea2c5089b590d08f1f3cdf2d72c4ec41666e37 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 27 Oct 2023 10:44:01 +0200 Subject: [PATCH 145/387] update emit --- subworkflows/local/variant_calling/call_sv_cnvnator.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/variant_calling/call_sv_cnvnator.nf b/subworkflows/local/variant_calling/call_sv_cnvnator.nf index 2c6db643..0d5c5423 100644 --- a/subworkflows/local/variant_calling/call_sv_cnvnator.nf +++ b/subworkflows/local/variant_calling/call_sv_cnvnator.nf @@ -46,6 +46,6 @@ workflow CALL_SV_CNVNATOR { ch_versions = ch_versions.mix(CNVNATOR_CONVERT2VCF.out.versions) emit: - vcf = CNVNATOR_CONVERT2VCF.out.vcf // channel: [ val(meta), path(*.tar.gz) ] + vcf = SVDB_MERGE_CNVNATOR.out.vcf // channel: [ val(meta), path(*.tar.gz) ] versions = ch_versions // channel: [ versions.yml ] } From efddf0b8f9593f535d41d533320bc4db1a30c7bb Mon Sep 17 00:00:00 2001 From: peterpru Date: Mon, 6 Nov 2023 13:03:25 +0100 Subject: [PATCH 146/387] initial commit to log progress --- modules/local/retroseq/call/placeholder.txt | 1 + modules/local/retroseq/discover/placeholder.txt | 1 + 2 files changed, 2 insertions(+) create mode 100644 modules/local/retroseq/call/placeholder.txt create mode 100644 modules/local/retroseq/discover/placeholder.txt diff --git a/modules/local/retroseq/call/placeholder.txt b/modules/local/retroseq/call/placeholder.txt new file mode 100644 index 00000000..b3a42524 --- /dev/null +++ b/modules/local/retroseq/call/placeholder.txt @@ -0,0 +1 @@ +placeholder \ No newline at end of file diff --git a/modules/local/retroseq/discover/placeholder.txt b/modules/local/retroseq/discover/placeholder.txt new file mode 100644 index 00000000..b3a42524 --- /dev/null +++ b/modules/local/retroseq/discover/placeholder.txt @@ -0,0 +1 @@ +placeholder \ No newline at end of file From 46041f37b849ac5a86c647a96109786f8ddc3c0b Mon Sep 17 00:00:00 2001 From: peterpru Date: Tue, 7 Nov 2023 10:43:00 +0100 Subject: [PATCH 147/387] add modules retroseq call and discover --- modules/local/retroseq/call/main.nf | 51 ++++++++++++++++++ modules/local/retroseq/call/meta.yml | 51 ++++++++++++++++++ modules/local/retroseq/call/placeholder.txt | 1 - modules/local/retroseq/discover/main.nf | 52 ++++++++++++++++++ modules/local/retroseq/discover/meta.yml | 54 +++++++++++++++++++ .../local/retroseq/discover/placeholder.txt | 1 - 6 files changed, 208 insertions(+), 2 deletions(-) create mode 100644 modules/local/retroseq/call/main.nf create mode 100644 modules/local/retroseq/call/meta.yml delete mode 100644 modules/local/retroseq/call/placeholder.txt create mode 100644 modules/local/retroseq/discover/main.nf create mode 100644 modules/local/retroseq/discover/meta.yml delete mode 100644 modules/local/retroseq/discover/placeholder.txt diff --git a/modules/local/retroseq/call/main.nf b/modules/local/retroseq/call/main.nf new file mode 100644 index 00000000..9e38e66c --- /dev/null +++ b/modules/local/retroseq/call/main.nf @@ -0,0 +1,51 @@ +process RETROSEQ_CALL { + tag "$meta.id" + label 'process_low' + + conda "bioconda::perl-retroseq=1.5=pl5321hdfd78af_1" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'docker://clinicalgenomics/retroseq:1.5_9d4f3b5' : 'docker://clinicalgenomics/retroseq:1.5_9d4f3b5' }" + + + input: + tuple val(meta), path(bam), path(bai) + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("*.vcf"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def VERSION = "1.5" + + """ + retroseq.pl \\ + -call \\ + -bam $bam \\ + -ref $fasta \\ + -output ${prefix}.tab + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + retroseq_call: $VERSION + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def VERSION = "1.5" + """ + touch ${prefix}.tab + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + retroseq_call: $VERSION + END_VERSIONS + """ +} diff --git a/modules/local/retroseq/call/meta.yml b/modules/local/retroseq/call/meta.yml new file mode 100644 index 00000000..7427491f --- /dev/null +++ b/modules/local/retroseq/call/meta.yml @@ -0,0 +1,51 @@ +name: "retroseq_call" +description: RetroSeq is a tool for discovery and genotyping of transposable element variants (TEVs) from next-gen sequencing reads aligned to a reference genome in BAM format. +keywords: + - retroseq + - transposable elements + - genomics +tools: + - "retroseq": + description: "RetroSeq: discovery and genotyping of TEVs from reads in BAM format." + homepage: "https://github.com/tk2/RetroSeq" + documentation: "https://github.com/tk2/RetroSeq" + tool_dev_url: "https://github.com/tk2/RetroSeq" + doi: "10.1093/bioinformatics/bts697" + licence: "['GPL']" + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - bam: + type: file + description: Sorted BAM file + pattern: "*.{bam}" + - bai: + type: file + description: Index of the sorted BAM file + pattern: "*.{bam}" + - fasta: + type: file + description: reference genome in fasta format + pattern: "*.{fasta}" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: Output file containing TEVs and their location in the genome. + pattern: "*.{vcf}" + +authors: + - "@peterpru" diff --git a/modules/local/retroseq/call/placeholder.txt b/modules/local/retroseq/call/placeholder.txt deleted file mode 100644 index b3a42524..00000000 --- a/modules/local/retroseq/call/placeholder.txt +++ /dev/null @@ -1 +0,0 @@ -placeholder \ No newline at end of file diff --git a/modules/local/retroseq/discover/main.nf b/modules/local/retroseq/discover/main.nf new file mode 100644 index 00000000..d71fd3fa --- /dev/null +++ b/modules/local/retroseq/discover/main.nf @@ -0,0 +1,52 @@ +process RETROSEQ_DISCOVER { + tag "$meta.id" + label 'process_low' + + conda "bioconda::perl-retroseq=1.5=pl5321hdfd78af_1" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'docker://clinicalgenomics/retroseq:1.5_9d4f3b5' : 'docker://clinicalgenomics/retroseq:1.5_9d4f3b5' }" + + + input: + tuple val(meta), path(bam), path(bai) + path(tab) + path(extra_files) + + output: + tuple val(meta), path("*.tab"), emit: tab + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def VERSION = "1.5" // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. + + """ + retroseq.pl \\ + -discover \\ + -bam $bam \\ + -refTEs $tab\\ + -output ${prefix}.tab + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + retroseq_discover: $VERSION + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def VERSION = "1.5" + """ + touch ${prefix}.tab + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + retroseq_discover: $VERSION + END_VERSIONS + """ +} diff --git a/modules/local/retroseq/discover/meta.yml b/modules/local/retroseq/discover/meta.yml new file mode 100644 index 00000000..a61f64c9 --- /dev/null +++ b/modules/local/retroseq/discover/meta.yml @@ -0,0 +1,54 @@ +name: "retroseq_discover" +description: RetroSeq is a tool for discovery and genotyping of transposable element variants (TEVs) from next-gen sequencing reads aligned to a reference genome in BAM format. +keywords: + - retroseq + - transposable elements + - genomics +tools: + - "retroseq": + description: "RetroSeq: discovery and genotyping of TEVs from reads in BAM format." + homepage: "https://github.com/tk2/RetroSeq" + documentation: "https://github.com/tk2/RetroSeq" + tool_dev_url: "https://github.com/tk2/RetroSeq" + doi: "10.1093/bioinformatics/bts697" + licence: "['GPL']" + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - bam: + type: file + description: Sorted BAM file + pattern: "*.{bam}" + - bai: + type: file + description: Index of the sorted BAM file + pattern: "*.{bam}" + - tab: + type: file + description: Tab file containing a list of files, where each line is a TE type and the path to a bed file containing its coordinates in the genome. + pattern: "*.{tab}" + - extra_files: + type: file + description: Each file contains the coordinates of transposable elements of a particular type. + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - tab: + type: file + description: Output file containing lists of read pair names per TE type + pattern: "*.{tab}" + +authors: + - "@peterpru" diff --git a/modules/local/retroseq/discover/placeholder.txt b/modules/local/retroseq/discover/placeholder.txt deleted file mode 100644 index b3a42524..00000000 --- a/modules/local/retroseq/discover/placeholder.txt +++ /dev/null @@ -1 +0,0 @@ -placeholder \ No newline at end of file From 0bcf05c7a9aadf925585f65e690983a641ff0097 Mon Sep 17 00:00:00 2001 From: peterpru Date: Tue, 7 Nov 2023 10:51:46 +0100 Subject: [PATCH 148/387] fix linting --- modules/local/retroseq/call/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/retroseq/call/main.nf b/modules/local/retroseq/call/main.nf index 9e38e66c..0fb36553 100644 --- a/modules/local/retroseq/call/main.nf +++ b/modules/local/retroseq/call/main.nf @@ -9,7 +9,7 @@ process RETROSEQ_CALL { input: tuple val(meta), path(bam), path(bai) - tuple val(meta), path(fasta) + tuple val(meta), path(fasta) output: tuple val(meta), path("*.vcf"), emit: vcf From d803ed8b9749b2b182651d5e44e208ea84455db1 Mon Sep 17 00:00:00 2001 From: peterpru Date: Tue, 7 Nov 2023 13:26:06 +0100 Subject: [PATCH 149/387] add subworkflow --- modules/local/retroseq/call/main.nf | 1 + modules/local/retroseq/discover/meta.yml | 2 +- .../local/annotate_mobile_elements.nf | 46 +++++++++++++++++++ 3 files changed, 48 insertions(+), 1 deletion(-) create mode 100644 subworkflows/local/annotate_mobile_elements.nf diff --git a/modules/local/retroseq/call/main.nf b/modules/local/retroseq/call/main.nf index 0fb36553..569d84a2 100644 --- a/modules/local/retroseq/call/main.nf +++ b/modules/local/retroseq/call/main.nf @@ -27,6 +27,7 @@ process RETROSEQ_CALL { retroseq.pl \\ -call \\ -bam $bam \\ + -input $input -ref $fasta \\ -output ${prefix}.tab diff --git a/modules/local/retroseq/discover/meta.yml b/modules/local/retroseq/discover/meta.yml index a61f64c9..80c6af6c 100644 --- a/modules/local/retroseq/discover/meta.yml +++ b/modules/local/retroseq/discover/meta.yml @@ -33,7 +33,7 @@ input: pattern: "*.{tab}" - extra_files: type: file - description: Each file contains the coordinates of transposable elements of a particular type. + description: List of files. Each file contains the coordinates of transposable elements of a particular type. output: - meta: diff --git a/subworkflows/local/annotate_mobile_elements.nf b/subworkflows/local/annotate_mobile_elements.nf new file mode 100644 index 00000000..26648434 --- /dev/null +++ b/subworkflows/local/annotate_mobile_elements.nf @@ -0,0 +1,46 @@ +// +// A subworkflow to annotate mobile elements in the genome +// + +include { RETROSEQ_CALL as RETROSEQ_CALL } from '../../modules/local/retroseq/call/main' +include { RETROSEQ_DISCOVER as RETROSEQ_DISCOVER } from '../../modules/local/retroseq/discover/main' + +workflow ANNOTATE_STRUCTURAL_VARIANTS { + + take: + ch_bam // channel: [mandatory] [ val(meta), path(bam) ] + ch_bai // channel: [mandatory] [ val(meta), path(bai) ] + ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] + + main: + ch_versions = Channel.empty() + + // Running retroseq discover: identify discordant read pairs that might support a TE insertion + RETROSEQ_DISCOVER ( + ch_bam_bai, + "tab", + "extrafiles" + ) + + // Running retroseq call: clusters reads and checks on the breakpoints to decide whether a TEV is present + RETROSEQ_CALL ( + ch_bam_bai, + ch_genome_fasta, + ) + + // Run vep to annotate + + // Run svdb to query against database + + // Filter and rank as done in findtroll? E.g. protein coding + + ch_versions = ch_versions.mix(RETROSEQ_CALL.out.versions) + ch_versions = ch_versions.mix(RETROSEQ_DISCOVER.out.versions) + + emit: + vcf_ann = RETROSEQ_CALL.out.vcf // channel: [ val(meta), path(vcf) ] + versions = ch_versions // channel: [ path(versions.yml) ] +} + From b80ed165380cabc131a5fc7cdf00990868464c24 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 13 Nov 2023 18:11:52 +0100 Subject: [PATCH 150/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index f4d17c8e..ccf9e781 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -14,6 +14,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New parameters to skip qualimap and eklipse (`--skip_qualimap` and `--skip_eklipse`) [#436](https://github.com/nf-core/raredisease/pull/436) - Fix "there is no process matching config selector warnings" [#435](https://github.com/nf-core/raredisease/pull/435) - New parameters to skip fastqc and haplocheck (`--skip_fastqc` and `--skip_haplocheck`) [#438](https://github.com/nf-core/raredisease/pull/438) +- CNVnator for copy number variant calling [#438](https://github.com/nf-core/raredisease/pull/434) ### `Changed` From 239a75c0ae27cc43850d5ce89bce889f47ef75a4 Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 14 Nov 2023 09:29:00 +0100 Subject: [PATCH 151/387] starting out --- modules.json | 5 ++ modules/nf-core/vcf2cytosure/environment.yml | 6 ++ modules/nf-core/vcf2cytosure/main.nf | 66 ++++++++++++++++ modules/nf-core/vcf2cytosure/meta.yml | 77 +++++++++++++++++++ subworkflows/local/generate_cytosure_files.nf | 34 ++++++++ 5 files changed, 188 insertions(+) create mode 100644 modules/nf-core/vcf2cytosure/environment.yml create mode 100644 modules/nf-core/vcf2cytosure/main.nf create mode 100644 modules/nf-core/vcf2cytosure/meta.yml create mode 100644 subworkflows/local/generate_cytosure_files.nf diff --git a/modules.json b/modules.json index db78a291..0b7c2a93 100644 --- a/modules.json +++ b/modules.json @@ -460,6 +460,11 @@ "git_sha": "9b159849d74f0eef251168c81c16da08215bbad5", "installed_by": ["modules"] }, + "vcf2cytosure": { + "branch": "master", + "git_sha": "2dba354d7c0a43a1e6d6ce26f408b4936d4ed619", + "installed_by": ["modules"] + }, "vcfanno": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", diff --git a/modules/nf-core/vcf2cytosure/environment.yml b/modules/nf-core/vcf2cytosure/environment.yml new file mode 100644 index 00000000..30e0542f --- /dev/null +++ b/modules/nf-core/vcf2cytosure/environment.yml @@ -0,0 +1,6 @@ +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::vcf2cytosure=0.9.1 diff --git a/modules/nf-core/vcf2cytosure/main.nf b/modules/nf-core/vcf2cytosure/main.nf new file mode 100644 index 00000000..f85dc05e --- /dev/null +++ b/modules/nf-core/vcf2cytosure/main.nf @@ -0,0 +1,66 @@ +process VCF2CYTOSURE { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/vcf2cytosure:0.9.1--pyh7cba7a3_1': + 'biocontainers/vcf2cytosure:0.9.1--pyh7cba7a3_1' }" + + input: + tuple val(meta), path(sv_vcf) + tuple val(meta2), path(coverage_bed) + tuple val(meta3), path(cns) + tuple val(meta4), path(snv_vcf) + path blacklist_bed + + output: + tuple val(meta), path("*.cgh"), emit: cgh + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def coverage = coverage_bed ? "--coverage ${coverage_bed}" : '' + def cnvkit = cns ? ( coverage_bed ? '' : "--cn ${cns}" ) : '' + def snv = snv_vcf ? ( coverage_bed ? '' : "--snv ${snv_vcf}" ) : '' + def blacklist = blacklist_bed ? "--blacklist ${blacklist_bed}" : '' + def prefix = task.ext.prefix ?: sv_vcf ? "${meta.id}" : "${meta3.id}" + + if ( cns && coverage_bed || snv_vcf && coverage_bed ) error "Coverage_bed input is not compatible with cns and snv" + + """ + vcf2cytosure \\ + --vcf $sv_vcf \\ + --out ${prefix}.cgh \\ + $coverage \\ + $cnvkit \\ + $snv \\ + $blacklist \\ + $args + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + vcf2cytosure: \$(echo \$(vcf2cytosure --version 2>&1) | sed 's/^.* cytosure //' ) + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def coverage = coverage_bed ? "--coverage ${coverage_bed}" : '' + def cnvkit = cns ? ( coverage_bed ? '' : "--cn ${cns}" ) : '' + def snv = snv_vcf ? ( coverage_bed ? '' : "--snv ${snv_vcf}" ) : '' + def blacklist = blacklist_bed ? "--blacklist ${blacklist_bed}" : '' + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + touch ${prefix}.xml + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + vcf2cytosure: \$(echo \$(vcf2cytosure --version 2>&1) | sed 's/^.* cytosure //' ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/vcf2cytosure/meta.yml b/modules/nf-core/vcf2cytosure/meta.yml new file mode 100644 index 00000000..39badd4c --- /dev/null +++ b/modules/nf-core/vcf2cytosure/meta.yml @@ -0,0 +1,77 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json +name: "vcf2cytosure" +description: Convert VCF with structural variations to CytoSure format +keywords: + - structural_variants + - array_cgh + - vcf + - cytosure +tools: + - "vcf2cytosure": + description: "Convert VCF with structural variations to CytoSure format" + homepage: "https://github.com/NBISweden/vcf2cytosure" + documentation: "https://github.com/NBISweden/vcf2cytosure" + tool_dev_url: "https://github.com/NBISweden/vcf2cytosure" + licence: "['MIT']" + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - meta2: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - meta3: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - meta4: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - sv_vcf: + type: file + description: VCF file with structural variants + pattern: "*.{vcf,vcf.gz}" + - coverage_bed: + type: file + description: Bed file with coverage data + pattern: "*.bed" + - cns: + type: file + description: CN file from CNVkit, not compatible with coverage_bed file + - snv_vcf: + type: file + description: | + VCF file with SNVs to calculate probe coverage, + not compatible with coverage_bed + pattern: "*.{vcf,vcf.gz}" + - blacklilst_bed: + type: file + description: Bed file with regions to exclude + pattern: "*.bed" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - cgh: + type: file + description: SV:s in CytoSure format + pattern: "*.cgh" + +authors: + - "@jemten" diff --git a/subworkflows/local/generate_cytosure_files.nf b/subworkflows/local/generate_cytosure_files.nf new file mode 100644 index 00000000..200acb6b --- /dev/null +++ b/subworkflows/local/generate_cytosure_files.nf @@ -0,0 +1,34 @@ +// +// Convert VCF with structural variations to the “.CGH” format used by the CytoSure Interpret Software +// + +include { TIDDIT_COV } from '../../modules/nf-core/tiddit/cov/main' +include { VCF2CYTOSURE } from '../modules/nf-core/vcf2cytosure/main' + +workflow SV_VCF_TO_CYTOSURE { + take: + ch_vcf // channel: [mandatory] [ val(meta), path(vcf), path(vcf_index) ] + ch_bam // channel: [mandatory] [ path(bam) ] + + main: + ch_versions = Channel.empty() + + TIDDIT_COV (ch_bam, [[],[]]) // 2nd pos. arg is req. only for cram input + + ch_vcf.dump(tag: 'vcf_channel', pretty: true) + + + + // Split vcf in samples combine and drop + + // run vcf2cytosure samplevise + + + [id:sample, [vcf]] + + + ch_versions = ch_versions.mix(TIDDIT_COV.out.versions.first()) + + emit: + versions = ch_versions // channel: [ versions.yml ] +} From e155b459f4ecca1640b188f486659d73e4a3cb86 Mon Sep 17 00:00:00 2001 From: Anders Jemt Date: Tue, 14 Nov 2023 10:30:32 +0100 Subject: [PATCH 152/387] fixes #439 --- docs/usage.md | 8 +++++--- 1 file changed, 5 insertions(+), 3 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 311dc349..75a8a1e5 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -176,9 +176,11 @@ The mandatory and optional parameters for each category are tabulated below. ##### 3. Repeat expansions -| Mandatory | Optional | -| --------------- | -------- | -| variant_catalog | | +| Mandatory | Optional | +| --------------------------- | -------- | +| variant_catalog1 | | + +1 We reccomend using the catalogs found [here](https://github.com/Clinical-Genomics/reference-files/tree/master/rare-disease/disease_loci/ExpansionHunter-v5.0.0). These catalogs have been extended from the illumina ones to include information on pathogenicity, which is neccesarry for the workflow. ##### 4. Variant calling - SNV From c475f3db91b07e48ff13e2172ddb8ddc3af511de Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 14 Nov 2023 13:52:15 +0100 Subject: [PATCH 153/387] addressing #429 --- CHANGELOG.md | 1 + conf/modules/annotate_genome_snvs.config | 2 +- docs/output.md | 4 ++++ 3 files changed, 6 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ccf9e781..a5d172cc 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -44,6 +44,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - If present, remove duplicate entries in probands and upd_children in the meta. [#420](https://github.com/nf-core/raredisease/pull/420) - Fixes vep starting as many instances as the square of the number of scatters. [#405](https://github.com/nf-core/raredisease/pull/405) - Replaced the logic where we added an arbitrary substring to keep file names unique after alignment which we then removed using a split operator, with a simple copy operation. [#425](https://github.com/nf-core/raredisease/pull/425/files) +- Preventing a crash of rhocall annotate in the case of running four individuals whereof two are affected. ### `Updated` diff --git a/conf/modules/annotate_genome_snvs.config b/conf/modules/annotate_genome_snvs.config index a68d83e9..6697c498 100644 --- a/conf/modules/annotate_genome_snvs.config +++ b/conf/modules/annotate_genome_snvs.config @@ -19,7 +19,7 @@ process { if (!params.skip_snv_annotation) { withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_ROH' { - ext.args = { "--samples ${meta.probands.join(",")} --skip-indels " } + ext.args = { "--samples ${meta.probands.unique().first()} --skip-indels " } ext.prefix = { "${meta.id}_roh" } } diff --git a/docs/output.md b/docs/output.md index 4da28214..53460628 100644 --- a/docs/output.md +++ b/docs/output.md @@ -314,6 +314,10 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support. [bcftools roh](https://samtools.github.io/bcftools/bcftools.html#roh) is a program for detecting runs of homo/autozygosity.from only bi-allelic sites. The output files are not published in the output folder, and is passed to vcfanno for further annotation. +:::note +In the case of running a quattro, i.e. two affected children and their parents, only one of the probands will be used for annotating regions of homozygosity. This is a know limitation that we are hoping to solve in a future release. +::: + #### vcfanno [vcfanno](https://github.com/brentp/vcfanno) allows you to quickly annotate your VCF with any number of INFO fields from any number of VCFs. It uses a simple configuration file to allow the user to specify the source annotation files and fields and how they will be added to the info of the query VCF. Values are pulled by name from the INFO field with special-cases of ID and FILTER to pull from those VCF columns. The output files are not published in the output folder, and is passed to CADD and/or VEP for further annotation. From b79abe50c6ff8752b7b99d35a184dc015bccd742 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 23 Nov 2023 11:37:38 +0100 Subject: [PATCH 154/387] updates --- modules.json | 4 +- modules/nf-core/eklipse/environment.yml | 7 ++ modules/nf-core/eklipse/main.nf | 3 +- modules/nf-core/eklipse/meta.yml | 5 +- modules/nf-core/eklipse/tests/main.nf.test | 36 ++++++++++ .../nf-core/eklipse/tests/main.nf.test.snap | 11 +++ modules/nf-core/eklipse/tests/tags.yml | 2 + modules/nf-core/svdb/query/environment.yml | 7 ++ modules/nf-core/svdb/query/main.nf | 24 ++++--- modules/nf-core/svdb/query/meta.yml | 10 ++- modules/nf-core/svdb/query/tests/main.nf.test | 36 ++++++++++ .../svdb/query/tests/main.nf.test.snap | 30 +++++++++ modules/nf-core/svdb/query/tests/tags.yml | 2 + nextflow_schema.json | 10 ++- .../local/annotate_structural_variants.nf | 67 ++++++++++++++++--- workflows/raredisease.nf | 1 + 16 files changed, 227 insertions(+), 28 deletions(-) create mode 100644 modules/nf-core/eklipse/environment.yml create mode 100644 modules/nf-core/eklipse/tests/main.nf.test create mode 100644 modules/nf-core/eklipse/tests/main.nf.test.snap create mode 100644 modules/nf-core/eklipse/tests/tags.yml create mode 100644 modules/nf-core/svdb/query/environment.yml create mode 100644 modules/nf-core/svdb/query/tests/main.nf.test create mode 100644 modules/nf-core/svdb/query/tests/main.nf.test.snap create mode 100644 modules/nf-core/svdb/query/tests/tags.yml diff --git a/modules.json b/modules.json index db78a291..e45a6fd8 100644 --- a/modules.json +++ b/modules.json @@ -102,7 +102,7 @@ }, "eklipse": { "branch": "master", - "git_sha": "39656f68219340420f03bd54a68e111c86e107e6", + "git_sha": "0031772de29283811deb989a5b42d0310d35ec22", "installed_by": ["modules"] }, "ensemblvep/filtervep": { @@ -417,7 +417,7 @@ }, "svdb/query": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "2a35cf4643135d51284236bc2835ee8b810c971d", "installed_by": ["modules"] }, "tabix/bgzip": { diff --git a/modules/nf-core/eklipse/environment.yml b/modules/nf-core/eklipse/environment.yml new file mode 100644 index 00000000..e3300709 --- /dev/null +++ b/modules/nf-core/eklipse/environment.yml @@ -0,0 +1,7 @@ +name: eklipse +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::eklipse=1.8 diff --git a/modules/nf-core/eklipse/main.nf b/modules/nf-core/eklipse/main.nf index 7b320a4b..b50df3c1 100644 --- a/modules/nf-core/eklipse/main.nf +++ b/modules/nf-core/eklipse/main.nf @@ -4,7 +4,7 @@ process EKLIPSE { label 'process_single' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. - conda "bioconda::eklipse=1.8" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/eklipse:1.8--hdfd78af_1': 'biocontainers/eklipse:1.8--hdfd78af_1' }" @@ -31,6 +31,7 @@ process EKLIPSE { echo "$bam\t${prefix}" > infile.txt eKLIPse.py \\ -in infile.txt \\ + $args \\ -ref $ref_gb mv eKLIPse_*/eKLIPse_deletions.csv eKLIPse_deletions.csv mv eKLIPse_*/eKLIPse_genes.csv eKLIPse_genes.csv diff --git a/modules/nf-core/eklipse/meta.yml b/modules/nf-core/eklipse/meta.yml index ee60ef65..0f5e88a8 100644 --- a/modules/nf-core/eklipse/meta.yml +++ b/modules/nf-core/eklipse/meta.yml @@ -17,7 +17,6 @@ tools: tool_dev_url: "https://github.com/dooguypapua/eKLIPse/tree/master" doi: "10.1038/s41436-018-0350-8" licence: ["GNU General Public v3 or later (GPL v3+)"] - input: - meta: type: map @@ -36,7 +35,6 @@ input: type: file description: mtDNA reference genome in Genbank format, optional if empty NC_012920.1.gb will be used pattern: "*.{gb}" - output: - meta: type: map @@ -55,6 +53,7 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@Lucpen" +maintainers: + - "@Lucpen" diff --git a/modules/nf-core/eklipse/tests/main.nf.test b/modules/nf-core/eklipse/tests/main.nf.test new file mode 100644 index 00000000..73cf1188 --- /dev/null +++ b/modules/nf-core/eklipse/tests/main.nf.test @@ -0,0 +1,36 @@ +nextflow_process { + + name "Test Process EKLIPSE" + script "../main.nf" + process "EKLIPSE" + tag "modules" + tag "modules_nfcore" + tag "eklipse" + + test("homo_sapiens [bam]") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_illumina_mt_bam'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_illumina_mt_bam_bai'], checkIfExists: true) + ] + input[1] = [ file(params.test_data['homo_sapiens']['genome']['genome_mt_gb'], checkIfExists: true) ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(file(process.out.deletions[0][1]), + file(process.out.genes[0][1]), + file(process.out.circos[0][1]), + file(process.out.versions[0])).match() + } + ) + } + } +} diff --git a/modules/nf-core/eklipse/tests/main.nf.test.snap b/modules/nf-core/eklipse/tests/main.nf.test.snap new file mode 100644 index 00000000..87d79dac --- /dev/null +++ b/modules/nf-core/eklipse/tests/main.nf.test.snap @@ -0,0 +1,11 @@ +{ + "eklipse": { + "content": [ + "eKLIPse_deletions.csv:md5,8bcfdb8f3e8a63dcd211c2a53b6ca0a7", + "eKLIPse_genes.csv:md5,ec1a1f31d018ca66a46d8a2d17bc8d18", + "eKLIPse_test.png:md5,d1ea2a548bc4f8d321b1128a61ea8b0a", + "versions.yml:md5,8e6ebaa9abf72ced91c1f56acd10f131" + ], + "timestamp": "2023-11-22T18:02:08.245264064" + } +} \ No newline at end of file diff --git a/modules/nf-core/eklipse/tests/tags.yml b/modules/nf-core/eklipse/tests/tags.yml new file mode 100644 index 00000000..eb91ee81 --- /dev/null +++ b/modules/nf-core/eklipse/tests/tags.yml @@ -0,0 +1,2 @@ +eklipse: + - modules/nf-core/eklipse/** diff --git a/modules/nf-core/svdb/query/environment.yml b/modules/nf-core/svdb/query/environment.yml new file mode 100644 index 00000000..bf048232 --- /dev/null +++ b/modules/nf-core/svdb/query/environment.yml @@ -0,0 +1,7 @@ +name: svdb_query +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::svdb=2.8.1 diff --git a/modules/nf-core/svdb/query/main.nf b/modules/nf-core/svdb/query/main.nf index 5dbd42ec..d516d382 100644 --- a/modules/nf-core/svdb/query/main.nf +++ b/modules/nf-core/svdb/query/main.nf @@ -2,7 +2,7 @@ process SVDB_QUERY { tag "$meta.id" label 'process_medium' - conda "bioconda::svdb=2.8.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/svdb:2.8.1--py39h5371cbf_0': 'biocontainers/svdb:2.8.1--py39h5371cbf_0' }" @@ -13,7 +13,8 @@ process SVDB_QUERY { val(in_frqs) val(out_occs) val(out_frqs) - path (vcf_dbs) + path(vcf_dbs) + path(bedpe_dbs) output: tuple val(meta), path("*_query.vcf") , emit: vcf @@ -23,12 +24,14 @@ process SVDB_QUERY { task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def in_occ = "" - def in_frq = "" - def out_occ = "" - def out_frq = "" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def in_occ = "" + def in_frq = "" + def out_occ = "" + def out_frq = "" + def dbs_argument = vcf_dbs ? "--db ${vcf_dbs.join(',')}" : '' + def bedpeds_argument = bedpe_dbs ? "--bedpedb ${bedpe_dbs.join(',')}" : '' if (in_occs) { in_occ = "--in_occ ${in_occs.join(',')}" } @@ -41,7 +44,7 @@ process SVDB_QUERY { if (out_frqs) { out_frq = "--out_frq ${out_frqs.join(',')}" } - + if ( vcf_dbs && bedpe_dbs ) error "bedpedb input is not compatible with db inputs" """ svdb \\ --query \\ @@ -50,7 +53,8 @@ process SVDB_QUERY { $out_occ \\ $out_frq \\ $args \\ - --db ${vcf_dbs.join(',')} \\ + $dbs_argument \\ + $bedpeds_argument \\ --query_vcf $vcf \\ --prefix ${prefix} diff --git a/modules/nf-core/svdb/query/meta.yml b/modules/nf-core/svdb/query/meta.yml index 57e67e15..d11c51e6 100644 --- a/modules/nf-core/svdb/query/meta.yml +++ b/modules/nf-core/svdb/query/meta.yml @@ -2,6 +2,8 @@ name: svdb_query description: Query a structural variant database, using a vcf file as query keywords: - structural variants + - query + - svdb tools: - svdb: description: structural variant database software @@ -25,10 +27,14 @@ input: type: file description: query vcf file pattern: "*.{vcf,vcf.gz}" - - vcf_db: + - vcf_dbs: type: file - description: database vcf file + description: path to a database vcf, or a comma separated list of vcfs pattern: "*.{vcf,vcf.gz}" + - bedpe_dbs: + type: file + description: path to a SV database of the following format chrA-posA-chrB-posB-type-count-frequency, or a comma separated list of files + pattern: "*.{bedpe}" output: - meta: diff --git a/modules/nf-core/svdb/query/tests/main.nf.test b/modules/nf-core/svdb/query/tests/main.nf.test new file mode 100644 index 00000000..edf801f7 --- /dev/null +++ b/modules/nf-core/svdb/query/tests/main.nf.test @@ -0,0 +1,36 @@ +nextflow_process { + + name "Test Process SVDB_QUERY" + script "modules/nf-core/svdb/query/main.nf" + process "SVDB_QUERY" + tag "modules" + tag "modules_nfcore" + tag "svdb" + tag "svdb/query" + + test("svdb query") { + + when { + process { + """ + input[0] = Channel.of([ [ id:'test' ], // meta map + [ file(params.test_data['homo_sapiens']['illumina']['test_sv_vcf'], checkIfExists: true) ] + ]) + input[1] = ['AC'] + input[2] = ['AF'] + input[3] = ['gnomad_svAC'] + input[4] = ['gnomad_svAF'] + input[5] = Channel.of([file(params.test_data['homo_sapiens']['genome']['gnomad_r2_1_1_sv_vcf_gz'], checkIfExists: true)]) + input[6] = [] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(file(process.out.vcf[0][1]).name, file(process.out.versions[0]).name).match()} + ) + } + } +} diff --git a/modules/nf-core/svdb/query/tests/main.nf.test.snap b/modules/nf-core/svdb/query/tests/main.nf.test.snap new file mode 100644 index 00000000..22021d0a --- /dev/null +++ b/modules/nf-core/svdb/query/tests/main.nf.test.snap @@ -0,0 +1,30 @@ +{ + "svdb query": { + "content": [ + "test_query.vcf", + "versions.yml" + ], + "timestamp": "2023-11-22T14:56:08.90223505" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,e10873514a6dfc4ff377d645e7b3c9f5" + ] + ], + "timestamp": "2023-11-22T14:36:48.543666873" + }, + "vcf": { + "content": [ + [ + [ + { + "id": "test" + }, + "test_query.vcf:md5,a29542606323b2430ec52ffb5c4c7f5e" + ] + ] + ], + "timestamp": "2023-11-22T14:36:48.523155905" + } +} \ No newline at end of file diff --git a/modules/nf-core/svdb/query/tests/tags.yml b/modules/nf-core/svdb/query/tests/tags.yml new file mode 100644 index 00000000..f4096378 --- /dev/null +++ b/modules/nf-core/svdb/query/tests/tags.yml @@ -0,0 +1,2 @@ +svdb/query: + - modules/nf-core/svdb/query/** diff --git a/nextflow_schema.json b/nextflow_schema.json index a03484d8..82618fca 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -288,11 +288,19 @@ "pattern": "^\\S+\\.dict$", "description": "Path to the genome dictionary file" }, + "svdb_query_bedpedbs": { + "type": "string", + "exists": true, + "format": "file-path", + "description": "Databases used for structural variant annotation in chrA-posA-chrB-posB-type-count-frequency format.", + "fa_icon": "fas fa-file-csv", + "help_text": "Path to comma-separated file containing information about the databases used for structural variant annotation." + }, "svdb_query_dbs": { "type": "string", "exists": true, "format": "file-path", - "description": "Databases used for structural variant annotation.", + "description": "Databases used for structural variant annotation in vcf format.", "fa_icon": "fas fa-file-csv", "help_text": "Path to comma-separated file containing information about the databases used for structural variant annotation." }, diff --git a/subworkflows/local/annotate_structural_variants.nf b/subworkflows/local/annotate_structural_variants.nf index be777ee2..e7046497 100644 --- a/subworkflows/local/annotate_structural_variants.nf +++ b/subworkflows/local/annotate_structural_variants.nf @@ -2,17 +2,19 @@ // A subworkflow to annotate structural variants. // -include { SVDB_QUERY } from '../../modules/nf-core/svdb/query/main' -include { PICARD_SORTVCF } from '../../modules/nf-core/picard/sortvcf/main' -include { BCFTOOLS_VIEW } from '../../modules/nf-core/bcftools/view/main' -include { ENSEMBLVEP as ENSEMBLVEP_SV } from '../../modules/local/ensemblvep/main' -include { TABIX_TABIX as TABIX_VEP } from '../../modules/nf-core/tabix/tabix/main' +include { SVDB_QUERY as SVDB_QUERY_DB } from '../../modules/nf-core/svdb/query/main' +include { SVDB_QUERY as SVDB_QUERY_BEDPE } from '../../modules/nf-core/svdb/query/main' +include { PICARD_SORTVCF } from '../../modules/nf-core/picard/sortvcf/main' +include { BCFTOOLS_VIEW } from '../../modules/nf-core/bcftools/view/main' +include { ENSEMBLVEP as ENSEMBLVEP_SV } from '../../modules/local/ensemblvep/main' +include { TABIX_TABIX as TABIX_VEP } from '../../modules/nf-core/tabix/tabix/main' workflow ANNOTATE_STRUCTURAL_VARIANTS { take: ch_vcf // channel: [mandatory] [ val(meta), path(vcf) ] ch_sv_dbs // channel: [mandatory] [ val(csv) ] + ch_sv_bedpedbs // channel: [mandatory] [ val(csv) ] val_vep_genome // string: [mandatory] GRCh37 or GRCh38 val_vep_cache_version // string: [mandatory] default: 107 ch_vep_cache // channel: [mandatory] [ path(cache) ] @@ -33,16 +35,62 @@ workflow ANNOTATE_STRUCTURAL_VARIANTS { } .set { ch_svdb_dbs } - SVDB_QUERY( + Channel.fromPath(ch_sv_bedpedbs) + .splitCsv ( header:true ) + .multiMap { row -> + bedpedbs: row.filename + in_frqs: row.in_freq_info_key + in_occs: row.in_allele_count_info_key + out_frqs: row.out_freq_info_key + out_occs: row.out_allele_count_info_key + } + .set { ch_svdb_bedpedbs } + + SVDB_QUERY_DB ( ch_vcf, ch_svdb_dbs.in_occs.toList(), ch_svdb_dbs.in_frqs.toList(), ch_svdb_dbs.out_occs.toList(), ch_svdb_dbs.out_frqs.toList(), - ch_svdb_dbs.vcf_dbs.toList() + ch_svdb_dbs.vcf_dbs.toList(), + [] ) - PICARD_SORTVCF(SVDB_QUERY.out.vcf, ch_genome_fasta, ch_genome_dictionary) + ch_vcf + .join(SVDB_QUERY_DB.out.vcf, remainder: true) + .branch { it -> + call: it[2].equals(null) + return [it[0], it[1]] + db: !(it[2].equals(null)) + return [it[0], it[2]] + } + .set { ch_for_mix_querydb } + + ch_querydb_out = ch_for_mix_querydb.call.mix(ch_for_mix_querydb.db) + + SVDB_QUERY_BEDPE ( + ch_querydb_out, + ch_svdb_bedpedbs.in_occs.toList(), + ch_svdb_bedpedbs.in_frqs.toList(), + ch_svdb_bedpedbs.out_occs.toList(), + ch_svdb_bedpedbs.out_frqs.toList(), + [], + ch_svdb_bedpedbs.bedpedbs.toList() + ) + + ch_querydb_out + .join(SVDB_QUERY_BEDPE.out.vcf, remainder: true) + .branch { it -> + db: it[2].equals(null) + return [it[0], it[1]] + bedped: !(it[2].equals(null)) + return [it[0], it[2]] + } + .set { ch_for_mix_querybedpedb } + + ch_querypedbed_out = ch_for_mix_querybedpedb.db.mix(ch_for_mix_querybedpedb.bedped) + + PICARD_SORTVCF(ch_querypedbed_out, ch_genome_fasta, ch_genome_dictionary) PICARD_SORTVCF.out.vcf.map { meta, vcf -> return [meta,vcf,[]] }.set { ch_sortvcf } @@ -60,7 +108,8 @@ workflow ANNOTATE_STRUCTURAL_VARIANTS { TABIX_VEP (ENSEMBLVEP_SV.out.vcf_gz) - ch_versions = ch_versions.mix(SVDB_QUERY.out.versions) + ch_versions = ch_versions.mix(SVDB_QUERY_DB.out.versions) + ch_versions = ch_versions.mix(SVDB_QUERY_BEDPE.out.versions) ch_versions = ch_versions.mix(PICARD_SORTVCF.out.versions) ch_versions = ch_versions.mix(BCFTOOLS_VIEW.out.versions) ch_versions = ch_versions.mix(ENSEMBLVEP_SV.out.versions) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index b528c0f7..0fec30dc 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -402,6 +402,7 @@ workflow RAREDISEASE { ANNOTATE_STRUCTURAL_VARIANTS ( CALL_STRUCTURAL_VARIANTS.out.vcf, params.svdb_query_dbs, + params.svdb_query_bedpedbs, params.genome, params.vep_cache_version, ch_vep_cache, From a3b5b9db58aed30d2a97e8d22268d33f3cbfa0d1 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 23 Nov 2023 14:03:17 +0100 Subject: [PATCH 155/387] fix errors --- conf/modules/annotate_structural_variants.config | 8 +++++++- docs/usage.md | 14 +++++++------- nextflow.config | 4 ++++ .../local/annotate_structural_variants.nf | 10 ++++++---- workflows/raredisease.nf | 16 ++++++++++++---- 5 files changed, 36 insertions(+), 16 deletions(-) diff --git a/conf/modules/annotate_structural_variants.config b/conf/modules/annotate_structural_variants.config index 4b3dfe7a..131149f9 100644 --- a/conf/modules/annotate_structural_variants.config +++ b/conf/modules/annotate_structural_variants.config @@ -24,7 +24,13 @@ process { ] } - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:SVDB_QUERY' { + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:SVDB_QUERY_BEDPE' { + ext.when = {!params.svdb_query_bedpedbs.equals(null)} + ext.prefix = { "${meta.id}_svdbquery" } + } + + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:SVDB_QUERY_DB' { + ext.when = {!params.svdb_query_dbs.equals(null)} ext.prefix = { "${meta.id}_svdbquery" } } diff --git a/docs/usage.md b/docs/usage.md index 75a8a1e5..beafeac2 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -242,13 +242,13 @@ no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sampl ##### 8. SV annotation & Ranking -| Mandatory | Optional | -| -------------------------- | ------------------ | -| genome | reduced_penetrance | -| svdb_query_dbs1 | | -| vep_cache_version | vep_filters | -| vep_cache | | -| score_config_sv | | +| Mandatory | Optional | +| ---------------------------------------------- | ------------------ | +| genome | reduced_penetrance | +| svdb_query_dbs/svdb_query_bedpedbs1 | | +| vep_cache_version | vep_filters | +| vep_cache | | +| score_config_sv | | 1 A CSV file that describes the databases (VCFs) used by SVDB for annotating structural variants. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/svdb_querydb_files.csv). Information about the column headers can be found [here](https://github.com/J35P312/SVDB#Query). diff --git a/nextflow.config b/nextflow.config index a6603a50..7309d888 100644 --- a/nextflow.config +++ b/nextflow.config @@ -37,6 +37,10 @@ params { cadd_resources = null platform = 'illumina' + // File params + svdb_query_bedpedbs = null + svdb_query_dbs = null + // Alignment aligner = 'bwamem2' rmdup = false diff --git a/subworkflows/local/annotate_structural_variants.nf b/subworkflows/local/annotate_structural_variants.nf index e7046497..43c8e97d 100644 --- a/subworkflows/local/annotate_structural_variants.nf +++ b/subworkflows/local/annotate_structural_variants.nf @@ -14,7 +14,7 @@ workflow ANNOTATE_STRUCTURAL_VARIANTS { take: ch_vcf // channel: [mandatory] [ val(meta), path(vcf) ] ch_sv_dbs // channel: [mandatory] [ val(csv) ] - ch_sv_bedpedbs // channel: [mandatory] [ val(csv) ] + ch_sv_bedpedbs // channel: [mandatory] [ val(csv) ] val_vep_genome // string: [mandatory] GRCh37 or GRCh38 val_vep_cache_version // string: [mandatory] default: 107 ch_vep_cache // channel: [mandatory] [ path(cache) ] @@ -22,9 +22,11 @@ workflow ANNOTATE_STRUCTURAL_VARIANTS { ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] main: - ch_versions = Channel.empty() + ch_versions = Channel.empty() + ch_svdb_dbs = Channel.empty() + ch_svdb_bedpedbs = Channel.empty() - Channel.fromPath(ch_sv_dbs) + ch_sv_dbs .splitCsv ( header:true ) .multiMap { row -> vcf_dbs: row.filename @@ -35,7 +37,7 @@ workflow ANNOTATE_STRUCTURAL_VARIANTS { } .set { ch_svdb_dbs } - Channel.fromPath(ch_sv_bedpedbs) + ch_sv_bedpedbs .splitCsv ( header:true ) .multiMap { row -> bedpedbs: row.filename diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 0fec30dc..4d1e51e7 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -32,6 +32,7 @@ def mandatoryParams = [ "variant_catalog", "variant_caller" ] +def missingParamsCount = 0 if (!params.skip_snv_annotation) { mandatoryParams += ["genome", "vcfanno_resources", "vcfanno_toml", "vep_cache", "vep_cache_version", @@ -39,7 +40,11 @@ if (!params.skip_snv_annotation) { } if (!params.skip_sv_annotation) { - mandatoryParams += ["genome", "svdb_query_dbs", "vep_cache", "vep_cache_version", "score_config_sv"] + mandatoryParams += ["genome", "vep_cache", "vep_cache_version", "score_config_sv"] + if (!params.svdb_query_bedpedbs && !params.svdb_query_dbs) { + println("params.svdb_query_bedpedbs or params.svdb_query_dbs should be set.") + missingParamsCount += 1 + } } if (!params.skip_mt_annotation) { @@ -62,7 +67,6 @@ if (!params.skip_vep_filter) { mandatoryParams += ["vep_filters"] } -def missingParamsCount = 0 for (param in mandatoryParams.unique()) { if (params[param] == null) { println("params." + param + " not set.") @@ -258,6 +262,10 @@ workflow RAREDISEASE { : Channel.value([]) ch_score_config_sv = params.score_config_sv ? Channel.fromPath(params.score_config_sv).collect() : Channel.value([]) + ch_sv_dbs = params.svdb_query_dbs ? Channel.fromPath(params.svdb_query_dbs) + : Channel.empty() + ch_sv_bedpedbs = params.svdb_query_bedpedbs ? Channel.fromPath(params.svdb_query_bedpedbs) + : Channel.empty() ch_target_bed = ch_references.target_bed ch_target_intervals = ch_references.target_intervals ch_variant_catalog = params.variant_catalog ? Channel.fromPath(params.variant_catalog).map { it -> [[id:it[0].simpleName],it]}.collect() @@ -401,8 +409,8 @@ workflow RAREDISEASE { if (!params.skip_sv_annotation) { ANNOTATE_STRUCTURAL_VARIANTS ( CALL_STRUCTURAL_VARIANTS.out.vcf, - params.svdb_query_dbs, - params.svdb_query_bedpedbs, + ch_sv_dbs, + ch_sv_bedpedbs, params.genome, params.vep_cache_version, ch_vep_cache, From 16ff843bf92c56854e627a182856172523aa06ac Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 23 Nov 2023 17:04:35 +0100 Subject: [PATCH 156/387] review comment --- subworkflows/local/annotate_structural_variants.nf | 12 ++++++------ 1 file changed, 6 insertions(+), 6 deletions(-) diff --git a/subworkflows/local/annotate_structural_variants.nf b/subworkflows/local/annotate_structural_variants.nf index 43c8e97d..6766a73c 100644 --- a/subworkflows/local/annotate_structural_variants.nf +++ b/subworkflows/local/annotate_structural_variants.nf @@ -61,14 +61,14 @@ workflow ANNOTATE_STRUCTURAL_VARIANTS { ch_vcf .join(SVDB_QUERY_DB.out.vcf, remainder: true) .branch { it -> - call: it[2].equals(null) + original_call: it[2].equals(null) return [it[0], it[1]] - db: !(it[2].equals(null)) + annotated_with_db: !(it[2].equals(null)) return [it[0], it[2]] } .set { ch_for_mix_querydb } - ch_querydb_out = ch_for_mix_querydb.call.mix(ch_for_mix_querydb.db) + ch_querydb_out = ch_for_mix_querydb.original_call.mix(ch_for_mix_querydb.annotated_with_db) SVDB_QUERY_BEDPE ( ch_querydb_out, @@ -83,14 +83,14 @@ workflow ANNOTATE_STRUCTURAL_VARIANTS { ch_querydb_out .join(SVDB_QUERY_BEDPE.out.vcf, remainder: true) .branch { it -> - db: it[2].equals(null) + querydb_out: it[2].equals(null) return [it[0], it[1]] - bedped: !(it[2].equals(null)) + annotated_with_bedped: !(it[2].equals(null)) return [it[0], it[2]] } .set { ch_for_mix_querybedpedb } - ch_querypedbed_out = ch_for_mix_querybedpedb.db.mix(ch_for_mix_querybedpedb.bedped) + ch_querypedbed_out = ch_for_mix_querybedpedb.querydb_out.mix(ch_for_mix_querybedpedb.annotated_with_bedped) PICARD_SORTVCF(ch_querypedbed_out, ch_genome_fasta, ch_genome_dictionary) From 3aa1e99817edbe03d406134f85a4aead195e1a9b Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 23 Nov 2023 21:35:42 +0530 Subject: [PATCH 157/387] Apply suggestions from code review Co-authored-by: Anders Jemt --- conf/modules/annotate_structural_variants.config | 4 ++-- docs/usage.md | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/conf/modules/annotate_structural_variants.config b/conf/modules/annotate_structural_variants.config index 131149f9..a7e60c69 100644 --- a/conf/modules/annotate_structural_variants.config +++ b/conf/modules/annotate_structural_variants.config @@ -25,12 +25,12 @@ process { } withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:SVDB_QUERY_BEDPE' { - ext.when = {!params.svdb_query_bedpedbs.equals(null)} + ext.when = { !params.svdb_query_bedpedbs.equals(null) } ext.prefix = { "${meta.id}_svdbquery" } } withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:SVDB_QUERY_DB' { - ext.when = {!params.svdb_query_dbs.equals(null)} + ext.when = { !params.svdb_query_dbs.equals(null) } ext.prefix = { "${meta.id}_svdbquery" } } diff --git a/docs/usage.md b/docs/usage.md index beafeac2..56d7fc6a 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -250,7 +250,7 @@ no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sampl | vep_cache | | | score_config_sv | | -1 A CSV file that describes the databases (VCFs) used by SVDB for annotating structural variants. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/svdb_querydb_files.csv). Information about the column headers can be found [here](https://github.com/J35P312/SVDB#Query). +1 A CSV file that describes the databases (VCFs or BEDPEs) used by SVDB for annotating structural variants. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/svdb_querydb_files.csv). Information about the column headers can be found [here](https://github.com/J35P312/SVDB#Query). ##### 9. Mitochondrial annotation From 0371db4e8babcc66cc76794b9cf99a0a545413de Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 24 Nov 2023 10:30:07 +0100 Subject: [PATCH 158/387] update config --- conf/modules/annotate_structural_variants.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/modules/annotate_structural_variants.config b/conf/modules/annotate_structural_variants.config index 131149f9..b2ee6218 100644 --- a/conf/modules/annotate_structural_variants.config +++ b/conf/modules/annotate_structural_variants.config @@ -26,12 +26,12 @@ process { withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:SVDB_QUERY_BEDPE' { ext.when = {!params.svdb_query_bedpedbs.equals(null)} - ext.prefix = { "${meta.id}_svdbquery" } + ext.prefix = { "${meta.id}_bedpedb" } } withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:SVDB_QUERY_DB' { ext.when = {!params.svdb_query_dbs.equals(null)} - ext.prefix = { "${meta.id}_svdbquery" } + ext.prefix = { "${meta.id}_vcfdb" } } withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:PICARD_SORTVCF' { From ec49262039ade915d66707335b93294fb4aab16a Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 24 Nov 2023 13:51:53 +0100 Subject: [PATCH 159/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index a5d172cc..90aec003 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -15,6 +15,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Fix "there is no process matching config selector warnings" [#435](https://github.com/nf-core/raredisease/pull/435) - New parameters to skip fastqc and haplocheck (`--skip_fastqc` and `--skip_haplocheck`) [#438](https://github.com/nf-core/raredisease/pull/438) - CNVnator for copy number variant calling [#438](https://github.com/nf-core/raredisease/pull/434) +- A new parameter `svdb_query_bedpedbs` to provide bedpe files as databases for SVDB query [#449](https://github.com/nf-core/raredisease/pull/449) ### `Changed` From a22602f4480ede2f6f9884a6286aae4f00df08f6 Mon Sep 17 00:00:00 2001 From: jemten Date: Fri, 1 Dec 2023 10:08:36 +0100 Subject: [PATCH 160/387] temp fix of mem allocation for gatk cnv --- modules.json | 6 ++++-- .../germlinecnvcaller/gatk4-germlinecnvcaller.diff | 14 ++++++++++++++ modules/nf-core/gatk4/germlinecnvcaller/main.nf | 2 +- .../gatk4-postprocessgermlinecnvcalls.diff | 14 ++++++++++++++ .../gatk4/postprocessgermlinecnvcalls/main.nf | 2 +- 5 files changed, 34 insertions(+), 4 deletions(-) create mode 100644 modules/nf-core/gatk4/germlinecnvcaller/gatk4-germlinecnvcaller.diff create mode 100644 modules/nf-core/gatk4/postprocessgermlinecnvcalls/gatk4-postprocessgermlinecnvcalls.diff diff --git a/modules.json b/modules.json index e45a6fd8..1c4709dd 100644 --- a/modules.json +++ b/modules.json @@ -153,7 +153,8 @@ "gatk4/germlinecnvcaller": { "branch": "master", "git_sha": "8c4542e5d421c4690cf1fa6ec729e9304763fdaf", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/gatk4/germlinecnvcaller/gatk4-germlinecnvcaller.diff" }, "gatk4/intervallisttools": { "branch": "master", @@ -178,7 +179,8 @@ "gatk4/postprocessgermlinecnvcalls": { "branch": "master", "git_sha": "8c4542e5d421c4690cf1fa6ec729e9304763fdaf", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/gatk4/postprocessgermlinecnvcalls/gatk4-postprocessgermlinecnvcalls.diff" }, "gatk4/preprocessintervals": { "branch": "master", diff --git a/modules/nf-core/gatk4/germlinecnvcaller/gatk4-germlinecnvcaller.diff b/modules/nf-core/gatk4/germlinecnvcaller/gatk4-germlinecnvcaller.diff new file mode 100644 index 00000000..c501e50e --- /dev/null +++ b/modules/nf-core/gatk4/germlinecnvcaller/gatk4-germlinecnvcaller.diff @@ -0,0 +1,14 @@ +Changes in module 'nf-core/gatk4/germlinecnvcaller' +--- modules/nf-core/gatk4/germlinecnvcaller/main.nf ++++ modules/nf-core/gatk4/germlinecnvcaller/main.nf +@@ -36,7 +36,7 @@ + avail_mem = (task.memory.mega*0.8).intValue() + } + """ +- gatk --java-options "-Xmx${avail_mem}g" GermlineCNVCaller \\ ++ gatk --java-options "-Xmx${avail_mem}M" GermlineCNVCaller \\ + $input_list \\ + $ploidy_command \\ + $output_command \\ + +************************************************************ diff --git a/modules/nf-core/gatk4/germlinecnvcaller/main.nf b/modules/nf-core/gatk4/germlinecnvcaller/main.nf index 973a0d3b..441e730f 100644 --- a/modules/nf-core/gatk4/germlinecnvcaller/main.nf +++ b/modules/nf-core/gatk4/germlinecnvcaller/main.nf @@ -36,7 +36,7 @@ process GATK4_GERMLINECNVCALLER { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}g" GermlineCNVCaller \\ + gatk --java-options "-Xmx${avail_mem}M" GermlineCNVCaller \\ $input_list \\ $ploidy_command \\ $output_command \\ diff --git a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/gatk4-postprocessgermlinecnvcalls.diff b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/gatk4-postprocessgermlinecnvcalls.diff new file mode 100644 index 00000000..b922c53d --- /dev/null +++ b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/gatk4-postprocessgermlinecnvcalls.diff @@ -0,0 +1,14 @@ +Changes in module 'nf-core/gatk4/postprocessgermlinecnvcalls' +--- modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf ++++ modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf +@@ -35,7 +35,7 @@ + avail_mem = (task.memory.mega*0.8).intValue() + } + """ +- gatk --java-options "-Xmx${avail_mem}g" PostprocessGermlineCNVCalls \\ ++ gatk --java-options "-Xmx${avail_mem}M" PostprocessGermlineCNVCalls \\ + $calls_command \\ + $model_command \\ + $ploidy_command \\ + +************************************************************ diff --git a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf index d6223043..d916c2af 100644 --- a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf +++ b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf @@ -35,7 +35,7 @@ process GATK4_POSTPROCESSGERMLINECNVCALLS { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}g" PostprocessGermlineCNVCalls \\ + gatk --java-options "-Xmx${avail_mem}M" PostprocessGermlineCNVCalls \\ $calls_command \\ $model_command \\ $ploidy_command \\ From 0d2a770ce7044f207228dd3fc525e6d8d9c8b388 Mon Sep 17 00:00:00 2001 From: jemten Date: Fri, 1 Dec 2023 10:14:34 +0100 Subject: [PATCH 161/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 90aec003..de991758 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -46,6 +46,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Fixes vep starting as many instances as the square of the number of scatters. [#405](https://github.com/nf-core/raredisease/pull/405) - Replaced the logic where we added an arbitrary substring to keep file names unique after alignment which we then removed using a split operator, with a simple copy operation. [#425](https://github.com/nf-core/raredisease/pull/425/files) - Preventing a crash of rhocall annotate in the case of running four individuals whereof two are affected. +- Fixed memory qualifier in gatk4 germlinecnvcaller and postprocessgermlinecnvcalls ### `Updated` From 6f24b3eab40da1b9ed9075ba31d600ac94ccccd6 Mon Sep 17 00:00:00 2001 From: lucpen Date: Thu, 14 Dec 2023 00:02:00 +0100 Subject: [PATCH 162/387] feat added ngsbits-samplegender --- CHANGELOG.md | 1 + CITATIONS.md | 2 + conf/modules/qc_bam.config | 10 +++ modules.json | 5 ++ .../ngsbits/samplegender/environment.yml | 7 ++ modules/nf-core/ngsbits/samplegender/main.nf | 51 ++++++++++++++ modules/nf-core/ngsbits/samplegender/meta.yml | 69 +++++++++++++++++++ subworkflows/local/qc_bam.nf | 6 ++ 8 files changed, 151 insertions(+) create mode 100644 modules/nf-core/ngsbits/samplegender/environment.yml create mode 100644 modules/nf-core/ngsbits/samplegender/main.nf create mode 100644 modules/nf-core/ngsbits/samplegender/meta.yml diff --git a/CHANGELOG.md b/CHANGELOG.md index de991758..58c9ac3b 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -16,6 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New parameters to skip fastqc and haplocheck (`--skip_fastqc` and `--skip_haplocheck`) [#438](https://github.com/nf-core/raredisease/pull/438) - CNVnator for copy number variant calling [#438](https://github.com/nf-core/raredisease/pull/434) - A new parameter `svdb_query_bedpedbs` to provide bedpe files as databases for SVDB query [#449](https://github.com/nf-core/raredisease/pull/449) +- ngsbits samplegender to check sex ### `Changed` diff --git a/CITATIONS.md b/CITATIONS.md index 5f404de8..71414414 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -80,6 +80,8 @@ > Pedersen BS, Quinlan AR. Mosdepth: quick coverage calculation for genomes and exomes. Hancock J, ed. Bioinformatics. 2018;34(5):867-868. doi:10.1093/bioinformatics/btx699 +- [ngs-bits-samplegender](https://github.com/imgag/ngs-bits/tree/master) + - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. diff --git a/conf/modules/qc_bam.config b/conf/modules/qc_bam.config index cc744773..0db1b255 100644 --- a/conf/modules/qc_bam.config +++ b/conf/modules/qc_bam.config @@ -53,6 +53,16 @@ process { ext.prefix = { "${meta.id}_mosdepth" } } + withName: '.*QC_BAM:NGSBITS_SAMPLEGENDER' { + // NGSBITS_SAMPLEGENDER needs a chrX and chrY in order to run so we skip it for the two test profiles + ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample', 'test_sentieon']).size() >= 1) || workflow.stubRun } + publishDir = [ + path: { "${params.outdir}/ngsbits_samplegender" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + withName: '.*QC_BAM:PICARD_COLLECTWGSMETRICS' { ext.when = { params.analysis_type.equals("wgs") && params.aligner.equals("bwamem2") } ext.prefix = { "${meta.id}_wgsmetrics" } diff --git a/modules.json b/modules.json index 1c4709dd..72c3e200 100644 --- a/modules.json +++ b/modules.json @@ -277,6 +277,11 @@ "git_sha": "a6e11ac655e744f7ebc724be669dd568ffdc0e80", "installed_by": ["modules"] }, + "ngsbits/samplegender": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, "peddy": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", diff --git a/modules/nf-core/ngsbits/samplegender/environment.yml b/modules/nf-core/ngsbits/samplegender/environment.yml new file mode 100644 index 00000000..486c233c --- /dev/null +++ b/modules/nf-core/ngsbits/samplegender/environment.yml @@ -0,0 +1,7 @@ +name: ngsbits_samplegender +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::ngs-bits=2023_02 diff --git a/modules/nf-core/ngsbits/samplegender/main.nf b/modules/nf-core/ngsbits/samplegender/main.nf new file mode 100644 index 00000000..3562238d --- /dev/null +++ b/modules/nf-core/ngsbits/samplegender/main.nf @@ -0,0 +1,51 @@ +process NGSBITS_SAMPLEGENDER { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ngs-bits:2023_02--py311ha0b7adc_2': + 'biocontainers/ngs-bits:2023_02--py311ha0b7adc_2' }" + + input: + tuple val(meta), path(bam), path(bai) + tuple val(meta2), path(fasta) + tuple val(meta2), path(fai) + val method + + output: + tuple val(meta), path("*.tsv"), emit: tsv + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def ref = fasta ? "-ref ${fasta}" : "" + """ + SampleGender \\ + -in ${bam} \\ + -method ${method} \\ + -out ${prefix}.tsv \\ + ${ref} \\ + ${args} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ngs-bits: \$(echo \$(SampleGender --version 2>&1) | sed 's/SampleGender //' ) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.tsv + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ngs-bits: \$(echo \$(SampleGender --version 2>&1) | sed 's/SampleGender //' ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/ngsbits/samplegender/meta.yml b/modules/nf-core/ngsbits/samplegender/meta.yml new file mode 100644 index 00000000..997bc06e --- /dev/null +++ b/modules/nf-core/ngsbits/samplegender/meta.yml @@ -0,0 +1,69 @@ +--- +name: "ngsbits_samplegender" +description: Determines the gender of a sample from the BAM/CRAM file. +keywords: + - gender + - cram + - bam + - short reads +tools: + - "ngsbits": + description: "Short-read sequencing tools" + homepage: "https://github.com/imgag/ngs-bits" + documentation: "https://github.com/imgag/ngs-bits" + tool_dev_url: "https://github.com/imgag/ngs-bits" + licence: "['MIT license']" +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: One or more BAM/CRAM files to determine the gender of + pattern: "*.{bam,cram}" + - bai: + type: file + description: The index file(s) from the input BAM/CRAM file(s) + pattern: "*.{bai,crai}" + - meta2: + type: map + description: | + Groovy Map containing reference fasta information + e.g. [ id:'test' ] + - fasta: + type: file + description: The reference FASTA to use (mandatory when CRAM files are used) + pattern: "*.{fasta,fa,fna}" + - meta3: + type: map + description: | + Groovy Map containing reference fasta index information + e.g. [ id:'test' ] + - fasta: + type: file + description: The index of the reference FASTA to use (mandatory when CRAM files are used) + pattern: "*.fai" + - method: + type: string + description: The method to use to define the gender (posibilities are 'xy', 'hetx' and 'sry') + pattern: "(xy|hetx|sry)" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - tsv: + type: file + description: An output TSV file containing the results of the gender prediction + pattern: "*.tsv" +authors: + - "@nvnieuwk" +maintainers: + - "@nvnieuwk" diff --git a/subworkflows/local/qc_bam.nf b/subworkflows/local/qc_bam.nf index 11439a7b..758ce7d7 100644 --- a/subworkflows/local/qc_bam.nf +++ b/subworkflows/local/qc_bam.nf @@ -12,6 +12,7 @@ include { PICARD_COLLECTWGSMETRICS as PICARD_COLLECTWGSMETRICS } from '../../m include { PICARD_COLLECTWGSMETRICS as PICARD_COLLECTWGSMETRICS_Y } from '../../modules/nf-core/picard/collectwgsmetrics/main' include { SENTIEON_WGSMETRICS } from '../../modules/nf-core/sentieon/wgsmetrics/main' include { SENTIEON_WGSMETRICS as SENTIEON_WGSMETRICS_Y } from '../../modules/nf-core/sentieon/wgsmetrics/main' +include { NGSBITS_SAMPLEGENDER } from '../../modules/nf-core/ngsbits/samplegender/main' workflow QC_BAM { @@ -59,6 +60,9 @@ workflow QC_BAM { SENTIEON_WGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_wgs.map{ interval -> [[:], interval]} ) SENTIEON_WGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_y.map{ interval -> [[:], interval]} ) + // Check sex + NGSBITS_SAMPLEGENDER(ch_bam_bai, ch_genome_fasta, ch_genome_fai, "xy") + ch_cov = Channel.empty().mix(PICARD_COLLECTWGSMETRICS.out.metrics, SENTIEON_WGSMETRICS.out.wgs_metrics) ch_cov_y = Channel.empty().mix(PICARD_COLLECTWGSMETRICS_Y.out.metrics, SENTIEON_WGSMETRICS_Y.out.wgs_metrics) @@ -67,6 +71,7 @@ workflow QC_BAM { ch_versions = ch_versions.mix(TIDDIT_COV.out.versions.first()) ch_versions = ch_versions.mix(UCSC_WIGTOBIGWIG.out.versions.first()) ch_versions = ch_versions.mix(MOSDEPTH.out.versions.first()) + ch_versions = ch_versions.mix(NGSBITS_SAMPLEGENDER.out.versions.first()) ch_versions = ch_versions.mix(PICARD_COLLECTWGSMETRICS.out.versions.first(), SENTIEON_WGSMETRICS.out.versions.first()) ch_versions = ch_versions.mix(PICARD_COLLECTWGSMETRICS_Y.out.versions.first(), SENTIEON_WGSMETRICS_Y.out.versions.first()) @@ -78,6 +83,7 @@ workflow QC_BAM { bigwig = UCSC_WIGTOBIGWIG.out.bw // channel: [ val(meta), path(bw) ] d4 = MOSDEPTH.out.per_base_d4 // channel: [ val(meta), path(d4) ] global_dist = MOSDEPTH.out.global_txt // channel: [ val(meta), path(txt) ] + sex_check = NGSBITS_SAMPLEGENDER.out.tsv // channel: [val(meta), path(tsv) ] cov = ch_cov // channel: [ val(meta), path(metrics) ] cov_y = ch_cov_y // channel: [ val(meta), path(metrics) ] versions = ch_versions // channel: [ path(versions.yml) ] From 4db892aae1c5958b985ab1b3491f25defc3e62db Mon Sep 17 00:00:00 2001 From: Alexander Koc Date: Fri, 15 Dec 2023 13:55:11 +0100 Subject: [PATCH 163/387] Fix wrong process name in sention BWA version output --- subworkflows/local/alignment/align_MT.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/alignment/align_MT.nf b/subworkflows/local/alignment/align_MT.nf index 11c51c6d..29903c72 100644 --- a/subworkflows/local/alignment/align_MT.nf +++ b/subworkflows/local/alignment/align_MT.nf @@ -32,7 +32,7 @@ workflow ALIGN_MT { } else if (params.aligner.equals("sentieon")) { SENTIEON_BWAMEM_MT ( ch_fastq, ch_bwaindex, ch_fasta, ch_fai ) ch_sentieon_bam = SENTIEON_BWAMEM_MT.out.bam_and_bai.map{ meta, bam, bai -> [meta, bam] } - ch_versions = ch_versions.mix(BWAMEM2_MEM_MT.out.versions.first()) + ch_versions = ch_versions.mix(SENTIEON_BWAMEM_MT.out.versions.first()) } Channel.empty() From c40c4adb5418e1e3e35e4307b45aeb7f6e219fe7 Mon Sep 17 00:00:00 2001 From: Alexander Koc Date: Fri, 15 Dec 2023 14:17:31 +0100 Subject: [PATCH 164/387] Fix wrong process name in ch_versions mix call --- subworkflows/local/call_snv.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index d363d35c..6413f896 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -61,7 +61,7 @@ workflow CALL_SNV { ) ch_sentieon_vcf = CALL_SNV_SENTIEON.out.vcf ch_sentieon_tbi = CALL_SNV_SENTIEON.out.tabix - ch_versions = ch_versions.mix(CALL_SNV_DEEPVARIANT.out.versions) + ch_versions = ch_versions.mix(CALL_SNV_SENTIEON.out.versions) } ch_vcf = Channel.empty().mix(ch_deepvar_vcf, ch_sentieon_vcf) From a4fe26cdaa40a9e802afa6247204ad5a27549fca Mon Sep 17 00:00:00 2001 From: Alexander Koc Date: Fri, 15 Dec 2023 16:19:45 +0100 Subject: [PATCH 165/387] Update CHANGELOG.md --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index de991758..bde103a2 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -47,6 +47,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Replaced the logic where we added an arbitrary substring to keep file names unique after alignment which we then removed using a split operator, with a simple copy operation. [#425](https://github.com/nf-core/raredisease/pull/425/files) - Preventing a crash of rhocall annotate in the case of running four individuals whereof two are affected. - Fixed memory qualifier in gatk4 germlinecnvcaller and postprocessgermlinecnvcalls +- Fixed wrong process names when outputting versions in `ALIGN_SENTIEON` and `CALL_SNV`. ### `Updated` From 0d952004fa3fd15fd32514ce141d861f2f058afb Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Luc=C3=ADa=20Pe=C3=B1a-P=C3=A9rez?= Date: Mon, 18 Dec 2023 09:14:23 +0100 Subject: [PATCH 166/387] Update CHANGELOG.md --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 58c9ac3b..7a0bff35 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -16,7 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New parameters to skip fastqc and haplocheck (`--skip_fastqc` and `--skip_haplocheck`) [#438](https://github.com/nf-core/raredisease/pull/438) - CNVnator for copy number variant calling [#438](https://github.com/nf-core/raredisease/pull/434) - A new parameter `svdb_query_bedpedbs` to provide bedpe files as databases for SVDB query [#449](https://github.com/nf-core/raredisease/pull/449) -- ngsbits samplegender to check sex +- ngsbits samplegender to check sex [#453] (https://github.com/nf-core/raredisease/pull/453) ### `Changed` From c12edd0bc846a0ede1cac060b05761ee210b20a3 Mon Sep 17 00:00:00 2001 From: lucpen Date: Mon, 18 Dec 2023 10:18:14 +0100 Subject: [PATCH 167/387] fix add prefix to ngs-bits output --- conf/modules/qc_bam.config | 1 + 1 file changed, 1 insertion(+) diff --git a/conf/modules/qc_bam.config b/conf/modules/qc_bam.config index 0db1b255..5140afa8 100644 --- a/conf/modules/qc_bam.config +++ b/conf/modules/qc_bam.config @@ -56,6 +56,7 @@ process { withName: '.*QC_BAM:NGSBITS_SAMPLEGENDER' { // NGSBITS_SAMPLEGENDER needs a chrX and chrY in order to run so we skip it for the two test profiles ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample', 'test_sentieon']).size() >= 1) || workflow.stubRun } + ext.prefix = { "${meta.id}_ngsbits_sex } publishDir = [ path: { "${params.outdir}/ngsbits_samplegender" }, mode: params.publish_dir_mode, From e2a6eb92fa87ceb1d4fda5c6c488e0e17195125d Mon Sep 17 00:00:00 2001 From: lucpen Date: Mon, 18 Dec 2023 10:22:34 +0100 Subject: [PATCH 168/387] fix add prefix to ngs-bits output --- conf/modules/qc_bam.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules/qc_bam.config b/conf/modules/qc_bam.config index 5140afa8..48e7d3c1 100644 --- a/conf/modules/qc_bam.config +++ b/conf/modules/qc_bam.config @@ -56,7 +56,7 @@ process { withName: '.*QC_BAM:NGSBITS_SAMPLEGENDER' { // NGSBITS_SAMPLEGENDER needs a chrX and chrY in order to run so we skip it for the two test profiles ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample', 'test_sentieon']).size() >= 1) || workflow.stubRun } - ext.prefix = { "${meta.id}_ngsbits_sex } + ext.prefix = { "${meta.id}_ngsbits_sex" } publishDir = [ path: { "${params.outdir}/ngsbits_samplegender" }, mode: params.publish_dir_mode, From 556e26f1c87ed5017d215a4d62e4bd2a33ae232f Mon Sep 17 00:00:00 2001 From: lucpen Date: Mon, 18 Dec 2023 12:04:51 +0100 Subject: [PATCH 169/387] feat added parameter for ngs-bits method --- main.nf | 1 + nextflow.config | 5 ++++- nextflow_schema.json | 9 ++++++++- subworkflows/local/qc_bam.nf | 23 ++++++++++++----------- workflows/raredisease.nf | 3 ++- 5 files changed, 27 insertions(+), 14 deletions(-) diff --git a/main.nf b/main.nf index 12cdadc0..ee02c024 100644 --- a/main.nf +++ b/main.nf @@ -35,6 +35,7 @@ params.known_dbsnp = WorkflowMain.getGenomeAttribute(params, params.known_dbsnp_tbi = WorkflowMain.getGenomeAttribute(params, 'known_dbsnp_tbi') params.ml_model = WorkflowMain.getGenomeAttribute(params, 'ml_model') params.mt_fasta = WorkflowMain.getGenomeAttribute(params, 'mt_fasta') +params.ngsbits_samplegender_method = WorkflowMain.getGenomeAttribute(params, 'ngsbits_samplegender_method') params.ploidy_model = WorkflowMain.getGenomeAttribute(params, 'ploidy_model') params.reduced_penetrance = WorkflowMain.getGenomeAttribute(params, 'reduced_penetrance') params.readcount_intervals = WorkflowMain.getGenomeAttribute(params, 'readcount_intervals') diff --git a/nextflow.config b/nextflow.config index 7309d888..6a58436a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -10,7 +10,7 @@ params { // Required options - input = null + input = null // References genome = 'GRCh38' @@ -37,6 +37,9 @@ params { cadd_resources = null platform = 'illumina' + // Bam_qc + ngsbits_samplegender_method = 'xy' + // File params svdb_query_bedpedbs = null svdb_query_dbs = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 82618fca..a5690fbb 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -386,6 +386,13 @@ "fa_icon": "fas fa-align-center", "enum": ["illumina"] }, + "ngsbits_samplegender_method": { + "type": "string", + "default": "xy", + "enum": ["xy", "hetx", "sry"], + "description": "Method selection for ngs-bits samplegender", + "fa_icon": "fas fa-align-center" + }, "save_mapped_as_cram": { "type": "boolean", "description": "Specifies whether to generate and publish alignment files as cram instead of bam", @@ -473,7 +480,7 @@ "cnvnator_binsize": { "type": "number", "description": "Bin size for CNVnator", - "default": "1000", + "default": 1000, "fa_icon": "fas fa-align-center" }, "sentieon_dnascope_pcr_indel_model": { diff --git a/subworkflows/local/qc_bam.nf b/subworkflows/local/qc_bam.nf index 758ce7d7..d4ae2842 100644 --- a/subworkflows/local/qc_bam.nf +++ b/subworkflows/local/qc_bam.nf @@ -17,16 +17,17 @@ include { NGSBITS_SAMPLEGENDER } from '../../m workflow QC_BAM { take: - ch_bam // channel: [mandatory] [ val(meta), path(bam) ] - ch_bai // channel: [mandatory] [ val(meta), path(bai) ] - ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] - ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] - ch_bait_intervals // channel: [mandatory] [ path(intervals_list) ] - ch_target_intervals // channel: [mandatory] [ path(intervals_list) ] - ch_chrom_sizes // channel: [mandatory] [ path(sizes) ] - ch_intervals_wgs // channel: [mandatory] [ path(intervals) ] - ch_intervals_y // channel: [mandatory] [ path(intervals) ] + ch_bam // channel: [mandatory] [ val(meta), path(bam) ] + ch_bai // channel: [mandatory] [ val(meta), path(bai) ] + ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_bait_intervals // channel: [mandatory] [ path(intervals_list) ] + ch_target_intervals // channel: [mandatory] [ path(intervals_list) ] + ch_chrom_sizes // channel: [mandatory] [ path(sizes) ] + ch_intervals_wgs // channel: [mandatory] [ path(intervals) ] + ch_intervals_y // channel: [mandatory] [ path(intervals) ] + ngsbits_samplegender_method // channel [val(method)] main: ch_versions = Channel.empty() @@ -61,7 +62,7 @@ workflow QC_BAM { SENTIEON_WGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_y.map{ interval -> [[:], interval]} ) // Check sex - NGSBITS_SAMPLEGENDER(ch_bam_bai, ch_genome_fasta, ch_genome_fai, "xy") + NGSBITS_SAMPLEGENDER(ch_bam_bai, ch_genome_fasta, ch_genome_fai, ngsbits_samplegender_method) ch_cov = Channel.empty().mix(PICARD_COLLECTWGSMETRICS.out.metrics, SENTIEON_WGSMETRICS.out.wgs_metrics) ch_cov_y = Channel.empty().mix(PICARD_COLLECTWGSMETRICS_Y.out.metrics, SENTIEON_WGSMETRICS_Y.out.wgs_metrics) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 4d1e51e7..c3acb0c7 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -339,7 +339,8 @@ workflow RAREDISEASE { ch_target_intervals, ch_genome_chrsizes, ch_intervals_wgs, - ch_intervals_y + ch_intervals_y, + Channel.value(params.ngsbits_samplegender_method) ) ch_versions = ch_versions.mix(QC_BAM.out.versions) From 7fa3f3a602f758dc3f49562c7eb027cecf419ef1 Mon Sep 17 00:00:00 2001 From: jemten Date: Mon, 18 Dec 2023 15:55:27 +0100 Subject: [PATCH 170/387] fixing vcf2cytosure subworkflow --- CHANGELOG.md | 1 + conf/modules/generate_cytosure_files.config | 50 +++++++++++++++++++ main.nf | 1 + modules.json | 3 +- modules/nf-core/vcf2cytosure/main.nf | 2 +- .../nf-core/vcf2cytosure/vcf2cytosure.diff | 14 ++++++ nextflow.config | 2 + nextflow_schema.json | 16 +++++- subworkflows/local/generate_cytosure_files.nf | 40 +++++++++------ workflows/raredisease.nf | 15 +++++- 10 files changed, 124 insertions(+), 20 deletions(-) create mode 100644 conf/modules/generate_cytosure_files.config create mode 100644 modules/nf-core/vcf2cytosure/vcf2cytosure.diff diff --git a/CHANGELOG.md b/CHANGELOG.md index de991758..c3f2b9c1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -16,6 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New parameters to skip fastqc and haplocheck (`--skip_fastqc` and `--skip_haplocheck`) [#438](https://github.com/nf-core/raredisease/pull/438) - CNVnator for copy number variant calling [#438](https://github.com/nf-core/raredisease/pull/434) - A new parameter `svdb_query_bedpedbs` to provide bedpe files as databases for SVDB query [#449](https://github.com/nf-core/raredisease/pull/449) +- New workflow for generating cgh files frm SV vcfs for interpretation in the CytosSure interpretation software. Turned off by default ### `Changed` diff --git a/conf/modules/generate_cytosure_files.config b/conf/modules/generate_cytosure_files.config new file mode 100644 index 00000000..e42717b1 --- /dev/null +++ b/conf/modules/generate_cytosure_files.config @@ -0,0 +1,50 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. + ext.when = Conditional clause +---------------------------------------------------------------------------------------- +*/ + +// +// VCF2Cytosure workflow options +// + +process { + + if ( params.analysis_type != "wes" && params.vcf2cytosure_switch ) { + + withName: '.*GENERATE_CYTOSURE_FILES:TIDDIT_COV_VCF2CYTOSURE' { + ext.args = { '-z 500' } + ext.prefix = { "${meta.id}_cov" } + } + + withName: '.*GENERATE_CYTOSURE_FILES:SPLIT_AND_FILTER_SV_VCF' { + ext.args = { [ + "--samples ${meta.id}", + '--output-type z', + "--exclude 'clingen_ngiAF > 0.1 || swegenAF > 0.05 || clinical_genomics_mipAF > 0.05 || decipherAF > 0.1 || clinical_genomics_loqusFrq > 0.05 || gnomad_svAF > 0.05'" + ].join(' ') } + ext.prefix = { ${meta.id} } + } + + withName: '.*GENERATE_CYTOSURE_FILES:VCF2CYTOSURE' { + ext.args = { [ + meta.sex.equals('1') ? '--sex male' : '--sex female', + '--size 5000', + '--maxbnd 5000' + ].join(' ') } + ext.prefix = { "${meta.id}" } + publishDir = [ + path: { "${params.outdir}/vcf2cytosure" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + ] + } + } +} diff --git a/main.nf b/main.nf index 12cdadc0..094fcd9c 100644 --- a/main.nf +++ b/main.nf @@ -46,6 +46,7 @@ params.svdb_query_dbs = WorkflowMain.getGenomeAttribute(params, params.target_bed = WorkflowMain.getGenomeAttribute(params, 'target_bed') params.variant_catalog = WorkflowMain.getGenomeAttribute(params, 'variant_catalog') params.vep_filters = WorkflowMain.getGenomeAttribute(params, 'vep_filters') +params.vcf2cytosure_blacklist = WorkflowMain.getGenomeAttribute(params, 'vcf2cytosure_blacklist') params.vcfanno_resources = WorkflowMain.getGenomeAttribute(params, 'vcfanno_resources') params.vcfanno_toml = WorkflowMain.getGenomeAttribute(params, 'vcfanno_toml') params.vcfanno_lua = WorkflowMain.getGenomeAttribute(params, 'vcfanno_lua') diff --git a/modules.json b/modules.json index b2dfa702..2fb1e307 100644 --- a/modules.json +++ b/modules.json @@ -465,7 +465,8 @@ "vcf2cytosure": { "branch": "master", "git_sha": "2dba354d7c0a43a1e6d6ce26f408b4936d4ed619", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/vcf2cytosure/vcf2cytosure.diff" }, "vcfanno": { "branch": "master", diff --git a/modules/nf-core/vcf2cytosure/main.nf b/modules/nf-core/vcf2cytosure/main.nf index f85dc05e..f253f979 100644 --- a/modules/nf-core/vcf2cytosure/main.nf +++ b/modules/nf-core/vcf2cytosure/main.nf @@ -12,7 +12,7 @@ process VCF2CYTOSURE { tuple val(meta2), path(coverage_bed) tuple val(meta3), path(cns) tuple val(meta4), path(snv_vcf) - path blacklist_bed + path(blacklist_bed) output: tuple val(meta), path("*.cgh"), emit: cgh diff --git a/modules/nf-core/vcf2cytosure/vcf2cytosure.diff b/modules/nf-core/vcf2cytosure/vcf2cytosure.diff new file mode 100644 index 00000000..660dfca7 --- /dev/null +++ b/modules/nf-core/vcf2cytosure/vcf2cytosure.diff @@ -0,0 +1,14 @@ +Changes in module 'nf-core/vcf2cytosure' +--- modules/nf-core/vcf2cytosure/main.nf ++++ modules/nf-core/vcf2cytosure/main.nf +@@ -12,7 +12,7 @@ + tuple val(meta2), path(coverage_bed) + tuple val(meta3), path(cns) + tuple val(meta4), path(snv_vcf) +- path blacklist_bed ++ path(blacklist_bed) + + output: + tuple val(meta), path("*.cgh"), emit: cgh + +************************************************************ diff --git a/nextflow.config b/nextflow.config index 7309d888..27543ad5 100644 --- a/nextflow.config +++ b/nextflow.config @@ -36,6 +36,7 @@ params { gens_switch = false cadd_resources = null platform = 'illumina' + vcf2cytosure_switch = false // File params svdb_query_bedpedbs = null @@ -299,6 +300,7 @@ includeConfig 'conf/modules/call_repeat_expansions.config' includeConfig 'conf/modules/call_snv.config' includeConfig 'conf/modules/call_structural_variants.config' includeConfig 'conf/modules/convert_mt_bam_to_fastq.config' +includeConfig 'conf/modules/generate_cytosure_files.config' includeConfig 'conf/modules/gens.config' includeConfig 'conf/modules/peddy_check.config' includeConfig 'conf/modules/prepare_references.config' diff --git a/nextflow_schema.json b/nextflow_schema.json index 82618fca..80cea991 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -321,6 +321,13 @@ "help_text": "Should be Stranger's extended JSON as described at https://github.com/Clinical-Genomics/stranger/blob/master/stranger/resources/variant_catalog_grch37.json. This file is used by both ExpansionHunter and Stranger", "fa_icon": "fas fa-file" }, + "vcf2cytosure_blacklist": { + "type": "string", + "help_text": "Optional file to blacklist regions for VCF2cytosure", + "pattern": "^\\S+\\.bed$", + "format": "file-path", + "fa_icon": "fas fa-file" + }, "vcfanno_resources": { "type": "string", "exists": true, @@ -435,6 +442,13 @@ "type": "boolean", "description": "Specifies whether or not to filter results based on a list of candidate genes specified in 'vep_filters'.", "fa_icon": "fas fa-toggle-on" + }, + "vcf2cytosure_switch": { + "type": "boolean", + "default": false, + "description": "Turn on the vcf2cytosure subworkflow", + "fa_icon": "fas fa-toggle-on", + "help_text": "vcf2cytosure can generate CGH files from a structural variant VCF file that can be analysed in the CytoSure interpretation software. Cut offs for allele frequencies and bin sizes can be modified in the config file. Turned off by default." } } }, @@ -473,7 +487,7 @@ "cnvnator_binsize": { "type": "number", "description": "Bin size for CNVnator", - "default": "1000", + "default": 1000, "fa_icon": "fas fa-align-center" }, "sentieon_dnascope_pcr_indel_model": { diff --git a/subworkflows/local/generate_cytosure_files.nf b/subworkflows/local/generate_cytosure_files.nf index 200acb6b..c5b4f205 100644 --- a/subworkflows/local/generate_cytosure_files.nf +++ b/subworkflows/local/generate_cytosure_files.nf @@ -2,33 +2,41 @@ // Convert VCF with structural variations to the “.CGH” format used by the CytoSure Interpret Software // -include { TIDDIT_COV } from '../../modules/nf-core/tiddit/cov/main' -include { VCF2CYTOSURE } from '../modules/nf-core/vcf2cytosure/main' +include { BCFTOOLS_VIEW as SPLIT_AND_FILTER_SV_VCF } from '../../modules/nf-core/bcftools/view/main' +include { TIDDIT_COV as TIDDIT_COV_VCF2CYTOSURE } from '../../modules/nf-core/tiddit/cov/main' +include { VCF2CYTOSURE } from '../../modules/nf-core/vcf2cytosure/main' -workflow SV_VCF_TO_CYTOSURE { +workflow GENERATE_CYTOSURE_FILES { take: ch_vcf // channel: [mandatory] [ val(meta), path(vcf), path(vcf_index) ] - ch_bam // channel: [mandatory] [ path(bam) ] + ch_bam // channel: [mandatory] [ val(meta), path(bam) ] + ch_blacklist // channel: [optional] [path(blacklist)] main: ch_versions = Channel.empty() - TIDDIT_COV (ch_bam, [[],[]]) // 2nd pos. arg is req. only for cram input + TIDDIT_COV_VCF2CYTOSURE (ch_bam, [[],[]]) - ch_vcf.dump(tag: 'vcf_channel', pretty: true) + // Build channel: [val(sample_meta). path(vcf), path(vcf_index)] + ch_bam.combine(ch_vcf).map { + meta_sample, bam, meta_case, vcf, tbi -> + return [ meta_sample, vcf, tbi ] + }.set { ch_sample_vcf } + // Split vcf into sample vcf:s and frequency filter + SPLIT_AND_FILTER_SV_VCF ( ch_sample_vcf, [], [], [] ) + VCF2CYTOSURE ( + SPLIT_AND_FILTER_SV_VCF.out.vcf, + TIDDIT_COV_VCF2CYTOSURE.out.cov, + [[], []], [[], []], + ch_blacklist + ) - // Split vcf in samples combine and drop - - // run vcf2cytosure samplevise - - - [id:sample, [vcf]] - - - ch_versions = ch_versions.mix(TIDDIT_COV.out.versions.first()) + ch_versions = ch_versions.mix(TIDDIT_COV_VCF2CYTOSURE.out.versions.first()) + ch_versions = ch_versions.mix(SPLIT_AND_FILTER_SV_VCF.out.versions.first()) + ch_versions = ch_versions.mix(VCF2CYTOSURE.out.versions.first()) emit: - versions = ch_versions // channel: [ versions.yml ] + versions = ch_versions // channel: [ versions.yml ] } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 4d1e51e7..7b21d492 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -124,14 +124,15 @@ include { ANNOTATE_STRUCTURAL_VARIANTS } from '../subworkflows/local/an include { CALL_REPEAT_EXPANSIONS } from '../subworkflows/local/call_repeat_expansions' include { CALL_SNV } from '../subworkflows/local/call_snv' include { CALL_STRUCTURAL_VARIANTS } from '../subworkflows/local/call_structural_variants' +include { GENERATE_CYTOSURE_FILES } from '../subworkflows/local/generate_cytosure_files' include { GENS } from '../subworkflows/local/gens' +include { PEDDY_CHECK } from '../subworkflows/local/peddy_check' include { PREPARE_REFERENCES } from '../subworkflows/local/prepare_references' include { QC_BAM } from '../subworkflows/local/qc_bam' include { RANK_VARIANTS as RANK_VARIANTS_MT } from '../subworkflows/local/rank_variants' include { RANK_VARIANTS as RANK_VARIANTS_SNV } from '../subworkflows/local/rank_variants' include { RANK_VARIANTS as RANK_VARIANTS_SV } from '../subworkflows/local/rank_variants' include { SCATTER_GENOME } from '../subworkflows/local/scatter_genome' -include { PEDDY_CHECK } from '../subworkflows/local/peddy_check' /* @@ -273,6 +274,8 @@ workflow RAREDISEASE { ch_variant_consequences = Channel.fromPath("$projectDir/assets/variant_consequences_v1.txt", checkIfExists: true).collect() ch_vcfanno_resources = params.vcfanno_resources ? Channel.fromPath(params.vcfanno_resources).splitText().map{it -> it.trim()}.collect() : Channel.value([]) + ch_vcf2cytosure_blacklist = params.vcf2cytosure_blacklist ? Channel.fromPath(params.vcf2cytosure_blacklist).collect() + : Channel.value([]) ch_vcfanno_lua = params.vcfanno_lua ? Channel.fromPath(params.vcfanno_lua).collect() : Channel.value([]) ch_vcfanno_toml = params.vcfanno_toml ? Channel.fromPath(params.vcfanno_toml).collect() @@ -565,6 +568,16 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(PEDDY_CHECK.out.versions) + // Generate CGH files from sequencing data, turned off by default + if ( params.vcf2cytosure_switch && params.analysis_type != "wes" ) { + GENERATE_CYTOSURE_FILES ( + BGZIPTABIX_SV.out.gz_tbi, + ch_mapped.genome_marked_bam, + ch_vcf2cytosure_blacklist + ) + ch_versions = ch_versions.mix(GENERATE_CYTOSURE_FILES.out.versions) + } + // GENS if (params.gens_switch) { GENS ( From 8b9d2014b3258ec293aad5d5a2384572e5b58418 Mon Sep 17 00:00:00 2001 From: lucpen Date: Mon, 18 Dec 2023 16:15:10 +0100 Subject: [PATCH 171/387] feat add fastp --- CHANGELOG.md | 1 + conf/modules/align.config | 15 + docs/usage.md | 12 +- main.nf | 1 + modules.json | 5 + modules/nf-core/fastp/environment.yml | 7 + modules/nf-core/fastp/main.nf | 102 ++++ modules/nf-core/fastp/meta.yml | 75 +++ modules/nf-core/fastp/tests/main.nf.test | 485 ++++++++++++++++++ modules/nf-core/fastp/tests/main.nf.test.snap | 52 ++ modules/nf-core/fastp/tests/nextflow.config | 6 + modules/nf-core/fastp/tests/tags.yml | 2 + nextflow.config | 4 +- nextflow_schema.json | 16 +- subworkflows/local/align.nf | 13 +- 15 files changed, 786 insertions(+), 10 deletions(-) create mode 100644 modules/nf-core/fastp/environment.yml create mode 100644 modules/nf-core/fastp/main.nf create mode 100644 modules/nf-core/fastp/meta.yml create mode 100644 modules/nf-core/fastp/tests/main.nf.test create mode 100644 modules/nf-core/fastp/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastp/tests/nextflow.config create mode 100644 modules/nf-core/fastp/tests/tags.yml diff --git a/CHANGELOG.md b/CHANGELOG.md index de991758..cf0089b1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -16,6 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New parameters to skip fastqc and haplocheck (`--skip_fastqc` and `--skip_haplocheck`) [#438](https://github.com/nf-core/raredisease/pull/438) - CNVnator for copy number variant calling [#438](https://github.com/nf-core/raredisease/pull/434) - A new parameter `svdb_query_bedpedbs` to provide bedpe files as databases for SVDB query [#449](https://github.com/nf-core/raredisease/pull/449) +- Fastp to do adapter trimming. It can be skipped using`--skip_fastp` ### `Changed` diff --git a/conf/modules/align.config b/conf/modules/align.config index d55b2e19..eb19e793 100644 --- a/conf/modules/align.config +++ b/conf/modules/align.config @@ -18,6 +18,21 @@ process{ ] } + if (!params.skip_fastp) { + withName: '.*ALIGN:FASTP' { + ext.args = [ + params.min_trimmed_length ? "--length_required ${params.min_trimmed_length}" : '', + '--correction', + '--overrepresentation_analysis' + ].join(' ').trim() + publishDir = [ + path: { "${params.outdir}/trimming" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + } + if (params.save_mapped_as_cram) { withName: '.*ALIGN:SAMTOOLS_VIEW' { ext.args = { '--output-fmt cram --write-index' } diff --git a/docs/usage.md b/docs/usage.md index 56d7fc6a..5e3b7fd2 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -152,16 +152,18 @@ The mandatory and optional parameters for each category are tabulated below. ##### 1. Alignment | Mandatory | Optional | -| ------------------------------ | --------------------------- | -| aligner1 | fasta_fai3 | -| fasta | bwamem23 | -| platform | known_dbsnp4 | -| mito_name/mt_fasta2 | known_dbsnp_tbi4 | +| ------------------------------ | ------------------------------ | +| aligner1 | fasta_fai3 | +| fasta | bwamem23 | +| platform | known_dbsnp4 | +| mito_name/mt_fasta2 | known_dbsnp_tbi4 | +| | min_trimmed_length5 | 1Default value is bwamem2, but if you have a valid license for Sentieon, you have the option to use Sentieon as well.
2f If mito_name is provided, mt_fasta can be generated by the pipeline.
3fasta_fai and bwamem2, if not provided by the user, will be generated by the pipeline when necessary.
4Used only by Sentieon.
+5Default value is 40. Used only by fastp.
##### 2. QC stats from the alignment files diff --git a/main.nf b/main.nf index 12cdadc0..32571801 100644 --- a/main.nf +++ b/main.nf @@ -44,6 +44,7 @@ params.score_config_snv = WorkflowMain.getGenomeAttribute(params, params.score_config_sv = WorkflowMain.getGenomeAttribute(params, 'score_config_sv') params.svdb_query_dbs = WorkflowMain.getGenomeAttribute(params, 'svdb_query_dbs') params.target_bed = WorkflowMain.getGenomeAttribute(params, 'target_bed') +params.min_trimmed_length = WorkflowMain.getGenomeAttribute(params, 'min_trimmed_length') params.variant_catalog = WorkflowMain.getGenomeAttribute(params, 'variant_catalog') params.vep_filters = WorkflowMain.getGenomeAttribute(params, 'vep_filters') params.vcfanno_resources = WorkflowMain.getGenomeAttribute(params, 'vcfanno_resources') diff --git a/modules.json b/modules.json index 1c4709dd..4a280808 100644 --- a/modules.json +++ b/modules.json @@ -115,6 +115,11 @@ "git_sha": "0260e5d22372eae434816d6970dedf3f5adc0053", "installed_by": ["modules"] }, + "fastp": { + "branch": "master", + "git_sha": "3c77ca9aac783e76c3614a06db3bfe4fef619bde", + "installed_by": ["modules"] + }, "fastqc": { "branch": "master", "git_sha": "bd8092b67b5103bdd52e300f75889442275c3117", diff --git a/modules/nf-core/fastp/environment.yml b/modules/nf-core/fastp/environment.yml new file mode 100644 index 00000000..70389e66 --- /dev/null +++ b/modules/nf-core/fastp/environment.yml @@ -0,0 +1,7 @@ +name: fastp +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastp=0.23.4 diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf new file mode 100644 index 00000000..5fac3c1a --- /dev/null +++ b/modules/nf-core/fastp/main.nf @@ -0,0 +1,102 @@ +process FASTP { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/fastp:0.23.4--h5f740d0_0' : + 'biocontainers/fastp:0.23.4--h5f740d0_0' }" + + input: + tuple val(meta), path(reads) + path adapter_fasta + val save_trimmed_fail + val save_merged + + output: + tuple val(meta), path('*.fastp.fastq.gz') , optional:true, emit: reads + tuple val(meta), path('*.json') , emit: json + tuple val(meta), path('*.html') , emit: html + tuple val(meta), path('*.log') , emit: log + path "versions.yml" , emit: versions + tuple val(meta), path('*.fail.fastq.gz') , optional:true, emit: reads_fail + tuple val(meta), path('*.merged.fastq.gz'), optional:true, emit: reads_merged + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def adapter_list = adapter_fasta ? "--adapter_fasta ${adapter_fasta}" : "" + def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : '' + // Added soft-links to original fastqs for consistent naming in MultiQC + // Use single ended for interleaved. Add --interleaved_in in config. + if ( task.ext.args?.contains('--interleaved_in') ) { + """ + [ ! -f ${prefix}.fastq.gz ] && ln -sf $reads ${prefix}.fastq.gz + + fastp \\ + --stdout \\ + --in1 ${prefix}.fastq.gz \\ + --thread $task.cpus \\ + --json ${prefix}.fastp.json \\ + --html ${prefix}.fastp.html \\ + $adapter_list \\ + $fail_fastq \\ + $args \\ + 2> >(tee ${prefix}.fastp.log >&2) \\ + | gzip -c > ${prefix}.fastp.fastq.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") + END_VERSIONS + """ + } else if (meta.single_end) { + """ + [ ! -f ${prefix}.fastq.gz ] && ln -sf $reads ${prefix}.fastq.gz + + fastp \\ + --in1 ${prefix}.fastq.gz \\ + --out1 ${prefix}.fastp.fastq.gz \\ + --thread $task.cpus \\ + --json ${prefix}.fastp.json \\ + --html ${prefix}.fastp.html \\ + $adapter_list \\ + $fail_fastq \\ + $args \\ + 2> >(tee ${prefix}.fastp.log >&2) + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") + END_VERSIONS + """ + } else { + def merge_fastq = save_merged ? "-m --merged_out ${prefix}.merged.fastq.gz" : '' + """ + [ ! -f ${prefix}_1.fastq.gz ] && ln -sf ${reads[0]} ${prefix}_1.fastq.gz + [ ! -f ${prefix}_2.fastq.gz ] && ln -sf ${reads[1]} ${prefix}_2.fastq.gz + fastp \\ + --in1 ${prefix}_1.fastq.gz \\ + --in2 ${prefix}_2.fastq.gz \\ + --out1 ${prefix}_1.fastp.fastq.gz \\ + --out2 ${prefix}_2.fastp.fastq.gz \\ + --json ${prefix}.fastp.json \\ + --html ${prefix}.fastp.html \\ + $adapter_list \\ + $fail_fastq \\ + $merge_fastq \\ + --thread $task.cpus \\ + --detect_adapter_for_pe \\ + $args \\ + 2> >(tee ${prefix}.fastp.log >&2) + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") + END_VERSIONS + """ + } +} diff --git a/modules/nf-core/fastp/meta.yml b/modules/nf-core/fastp/meta.yml new file mode 100644 index 00000000..c22a16ab --- /dev/null +++ b/modules/nf-core/fastp/meta.yml @@ -0,0 +1,75 @@ +name: fastp +description: Perform adapter/quality trimming on sequencing reads +keywords: + - trimming + - quality control + - fastq +tools: + - fastp: + description: | + A tool designed to provide fast all-in-one preprocessing for FastQ files. This tool is developed in C++ with multithreading supported to afford high performance. + documentation: https://github.com/OpenGene/fastp + doi: 10.1093/bioinformatics/bty560 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information. Use 'single_end: true' to specify single ended or interleaved FASTQs. Use 'single_end: false' for paired-end reads. + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. If you wish to run interleaved paired-end data, supply as single-end data + but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module. + - adapter_fasta: + type: file + description: File in FASTA format containing possible adapters to remove. + pattern: "*.{fasta,fna,fas,fa}" + - save_trimmed_fail: + type: boolean + description: Specify true to save files that failed to pass trimming thresholds ending in `*.fail.fastq.gz` + - save_merged: + type: boolean + description: Specify true to save all merged reads to the a file ending in `*.merged.fastq.gz` +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: The trimmed/modified/unmerged fastq reads + pattern: "*fastp.fastq.gz" + - json: + type: file + description: Results in JSON format + pattern: "*.json" + - html: + type: file + description: Results in HTML format + pattern: "*.html" + - log: + type: file + description: fastq log file + pattern: "*.log" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - reads_fail: + type: file + description: Reads the failed the preprocessing + pattern: "*fail.fastq.gz" + - reads_merged: + type: file + description: Reads that were successfully merged + pattern: "*.{merged.fastq.gz}" +authors: + - "@drpatelh" + - "@kevinmenden" +maintainers: + - "@drpatelh" + - "@kevinmenden" diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test new file mode 100644 index 00000000..f610b735 --- /dev/null +++ b/modules/nf-core/fastp/tests/main.nf.test @@ -0,0 +1,485 @@ +nextflow_process { + + name "Test Process FASTP" + script "../main.nf" + process "FASTP" + tag "modules" + tag "modules_nfcore" + tag "fastp" + + test("test_fastp_single_end") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = [ + [ id:'test', single_end:true ], + [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] + ] + + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:12.922000 K (92.984097%)", + "single end (151 cycles)" ] + def log_text = [ "Q20 bases: 12922(92.9841%)", + "reads passed filter: 99" ] + def read_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { assert snapshot(process.out.json).match("test_fastp_single_end_json") }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("test_fastp_paired_end") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] + ] + + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:25.719000 K (93.033098%)", + "The input has little adapter percentage (~0.000000%), probably it's trimmed before."] + def log_text = [ "No adapter detected for read1", + "Q30 bases: 12281(88.3716%)"] + def json_text = ['"passed_filter_reads": 198'] + def read1_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } + } + }, + { read2_lines.each { read2_line -> + { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { json_text.each { json_part -> + { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } + } + }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("fastp test_fastp_interleaved") { + config './nextflow.config' + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = [ [ id:'test', single_end:true ], // meta map + [ file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) ] + ] + + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:25.719000 K (93.033098%)", + "paired end (151 cycles + 151 cycles)"] + def log_text = [ "Q20 bases: 12922(92.9841%)", + "reads passed filter: 198"] + def read_lines = [ "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { assert snapshot(process.out.json).match("fastp test_fastp_interleaved_json") }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("test_fastp_single_end_trim_fail") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = true + save_merged = false + + input[0] = [ [ id:'test', single_end:true ], // meta map + [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] + ] + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:12.922000 K (92.984097%)", + "single end (151 cycles)"] + def log_text = [ "Q20 bases: 12922(92.9841%)", + "reads passed filter: 99" ] + def read_lines = [ "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) } + } + }, + { failed_read_lines.each { failed_read_line -> + { assert path(process.out.reads_fail.get(0).get(1)).linesGzip.contains(failed_read_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { assert snapshot(process.out.json).match("test_fastp_single_end_trim_fail_json") }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("test_fastp_paired_end_trim_fail") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = true + save_merged = false + + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:25.719000 K (93.033098%)", + "The input has little adapter percentage (~0.000000%), probably it's trimmed before."] + def log_text = [ "No adapter detected for read1", + "Q30 bases: 12281(88.3716%)"] + def json_text = ['"passed_filter_reads": 198'] + def read1_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } + } + }, + { read2_lines.each { read2_line -> + { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } + } + }, + { failed_read2_lines.each { failed_read2_line -> + { assert path(process.out.reads_fail.get(0).get(1).get(1)).linesGzip.contains(failed_read2_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { json_text.each { json_part -> + { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } + } + }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("test_fastp_paired_end_merged") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = true + + input[0] = [ [ id:'test', single_end:false ], // meta map + [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] + ] + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "
"] + def log_text = [ "Merged and filtered:", + "total reads: 75", + "total bases: 13683"] + def json_text = ['"merged_and_filtered": {', '"total_reads": 75', '"total_bases": 13683'] + def read1_lines = [ "@ERR5069949.1066259 NS500628:121:HK3MMAFX2:1:11312:18369:8333/1", + "CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC", + "AAAAAEAEEAEEEEEEEEEEEEEEEEAEEEEAEEEEEEEEAEEEEEEEEEEEEEEEEE/EAEEEEEE/6EEEEEEEEEEAEEAEEE/EE/AEEAEEEEEAEEEA/EEAAEAE + { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } + } + }, + { read2_lines.each { read2_line -> + { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } + } + }, + { read_merged_lines.each { read_merged_line -> + { assert path(process.out.reads_merged.get(0).get(1)).linesGzip.contains(read_merged_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { json_text.each { json_part -> + { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } + } + }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("test_fastp_paired_end_merged_adapterlist") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = file("https://github.com/nf-core/test-datasets/raw/modules/data/delete_me/fastp/adapters.fasta", checkIfExists: true) + save_trimmed_fail = false + save_merged = true + + input[0] = [ [ id:'test', single_end:false ], // meta map + [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] + ] + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "
"] + def log_text = [ "Merged and filtered:", + "total reads: 75", + "total bases: 13683"] + def json_text = ['"merged_and_filtered": {', '"total_reads": 75', '"total_bases": 13683',"--adapter_fasta"] + def read1_lines = ["@ERR5069949.1066259 NS500628:121:HK3MMAFX2:1:11312:18369:8333/1", + "CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC", + "AAAAAEAEEAEEEEEEEEEEEEEEEEAEEEEAEEEEEEEEAEEEEEEEEEEEEEEEEE/EAEEEEEE/6EEEEEEEEEEAEEAEEE/EE/AEEAEEEEEAEEEA/EEAAEAE + { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } + } + }, + { read2_lines.each { read2_line -> + { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } + } + }, + { read_merged_lines.each { read_merged_line -> + { assert path(process.out.reads_merged.get(0).get(1)).linesGzip.contains(read_merged_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { json_text.each { json_part -> + { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } + } + }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } +} diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap new file mode 100644 index 00000000..0fa68c7d --- /dev/null +++ b/modules/nf-core/fastp/tests/main.nf.test.snap @@ -0,0 +1,52 @@ +{ + "fastp test_fastp_interleaved_json": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastp.json:md5,168f516f7bd4b7b6c32da7cba87299a4" + ] + ] + ], + "timestamp": "2023-10-17T11:04:45.794175881" + }, + "test_fastp_single_end_json": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastp.json:md5,c852d7a6dba5819e4ac8d9673bedcacc" + ] + ] + ], + "timestamp": "2023-10-17T11:04:10.566343705" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "timestamp": "2023-10-17T11:04:10.582076024" + }, + "test_fastp_single_end_trim_fail_json": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastp.json:md5,9a7ee180f000e8d00c7fb67f06293eb5" + ] + ] + ], + "timestamp": "2023-10-17T11:05:00.379878948" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastp/tests/nextflow.config b/modules/nf-core/fastp/tests/nextflow.config new file mode 100644 index 00000000..0f7849ad --- /dev/null +++ b/modules/nf-core/fastp/tests/nextflow.config @@ -0,0 +1,6 @@ +process { + + withName: FASTP { + ext.args = "--interleaved_in" + } +} diff --git a/modules/nf-core/fastp/tests/tags.yml b/modules/nf-core/fastp/tests/tags.yml new file mode 100644 index 00000000..c1afcce7 --- /dev/null +++ b/modules/nf-core/fastp/tests/tags.yml @@ -0,0 +1,2 @@ +fastp: + - modules/nf-core/fastp/** diff --git a/nextflow.config b/nextflow.config index 7309d888..f0d7b751 100644 --- a/nextflow.config +++ b/nextflow.config @@ -25,6 +25,7 @@ params { bait_padding = 100 save_mapped_as_cram = false skip_eklipse = false + skip_fastp = false skip_fastqc = false skip_germlinecnvcaller = false skip_haplocheck = false @@ -43,6 +44,7 @@ params { // Alignment aligner = 'bwamem2' + min_trimmed_length = rmdup = false // Variant calling @@ -50,7 +52,7 @@ params { variant_caller = 'deepvariant' // sentieon Defaults - ml_model = '' + ml_model = 40 // Dnascope SNV calling sentieon_dnascope_pcr_indel_model = 'CONSERVATIVE' diff --git a/nextflow_schema.json b/nextflow_schema.json index 82618fca..9bfd4381 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -53,7 +53,7 @@ "properties": { "bait_padding": { "type": "number", - "default": 100, + "default": 100.0, "fa_icon": "fas fa-align-center", "pattern": "^\\S+\\.bed(\\.gz)?$", "description": "The amount to pad each end of the target intervals to create bait intervals." @@ -396,6 +396,11 @@ "description": "Specifies whether or not to skip FASTQC.", "fa_icon": "fas fa-toggle-on" }, + "skip_fastp": { + "type": "boolean", + "description": "Specifies whether or not to skip trimming with fastp.", + "fa_icon": "fas fa-toggle-on" + }, "skip_haplocheck": { "type": "boolean", "description": "Specifies whether or not to skip haplocheck.", @@ -451,6 +456,13 @@ "fa_icon": "fas fa-align-center", "enum": ["bwamem2", "sentieon"] }, + "min_trimmed_length": { + "type": "integer", + "default": 40, + "description": "Discard trimmed reads shorter than this", + "help_text": "Minimum length of reads after adapter trimming. Shorter reads are discarded. The program default is 15 bp. ", + "fa_icon": "fas fa-less-than" + }, "rmdup": { "type": "boolean", "description": "Specifies whether duplicates reads should be removed prior to variant calling.", @@ -473,7 +485,7 @@ "cnvnator_binsize": { "type": "number", "description": "Bin size for CNVnator", - "default": "1000", + "default": 1000.0, "fa_icon": "fas fa-align-center" }, "sentieon_dnascope_pcr_indel_model": { diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index 8e9baa2f..fa819926 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -2,6 +2,7 @@ // Map to reference // +include { FASTP } from '../../modules/nf-core/fastp/main' include { ALIGN_BWAMEM2 } from './alignment/align_bwamem2' include { ALIGN_SENTIEON } from './alignment/align_sentieon' include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main' @@ -31,9 +32,17 @@ workflow ALIGN { ch_bwamem2_bai = Channel.empty() ch_sentieon_bai = Channel.empty() + if (!params.skip_germlinecnvcaller) { + FASTP(ch_reads, [], false, false) + ch_input_reads = FASTP.out.reads + ch_versions = ch_versions.mix(FASTP.out.versions) + } else { ch_input_reads = ch_reads } + + //ch_input_reads.view() + if (params.aligner.equals("bwamem2")) { ALIGN_BWAMEM2 ( // Triggered when params.aligner is set as bwamem2 - ch_reads, + ch_input_reads, ch_genome_bwamem2index, ch_genome_fasta, ch_genome_fai, @@ -44,7 +53,7 @@ workflow ALIGN { ch_versions = ch_versions.mix(ALIGN_BWAMEM2.out.versions) } else if (params.aligner.equals("sentieon")) { ALIGN_SENTIEON ( // Triggered when params.aligner is set as sentieon - ch_reads, + ch_input_reads, ch_genome_fasta, ch_genome_fai, ch_genome_bwaindex, From 797cb17b2fb18bc2a7661d31d8f1efd2cf866ce9 Mon Sep 17 00:00:00 2001 From: lucpen Date: Mon, 18 Dec 2023 16:21:23 +0100 Subject: [PATCH 172/387] feat add fastp --- CHANGELOG.md | 2 +- CITATIONS.md | 3 +++ 2 files changed, 4 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index cf0089b1..53630e49 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -16,7 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New parameters to skip fastqc and haplocheck (`--skip_fastqc` and `--skip_haplocheck`) [#438](https://github.com/nf-core/raredisease/pull/438) - CNVnator for copy number variant calling [#438](https://github.com/nf-core/raredisease/pull/434) - A new parameter `svdb_query_bedpedbs` to provide bedpe files as databases for SVDB query [#449](https://github.com/nf-core/raredisease/pull/449) -- Fastp to do adapter trimming. It can be skipped using`--skip_fastp` +- Fastp to do adapter trimming. It can be skipped using`--skip_fastp`[#457](https://github.com/nf-core/raredisease/pull/457) ### `Changed` diff --git a/CITATIONS.md b/CITATIONS.md index 5f404de8..187405e5 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -48,6 +48,9 @@ > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. +- [Fastp](https://github.com/OpenGene/fastp) + > Shifu, C. (2023). Ultrafast one-pass FASTQ data preprocessing, quality control, and deduplication using fastp. iMeta 2: e107. https://doi.org/10.1002/imt2.107 + - [GATK](https://genome.cshlp.org/content/20/9/1297) > McKenna A, Hanna M, Banks E, et al. The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res. 2010;20(9):1297-1303. doi:10.1101/gr.107524.110 From 2791f1fefa03e4c41913c8af9970df54cbd78622 Mon Sep 17 00:00:00 2001 From: jemten Date: Mon, 18 Dec 2023 16:27:10 +0100 Subject: [PATCH 173/387] updates changelog and conf files --- CHANGELOG.md | 4 ++-- conf/modules/generate_cytosure_files.config | 2 +- nextflow_schema.json | 4 ++-- 3 files changed, 5 insertions(+), 5 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 127c9e89..bfd2c315 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -16,8 +16,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New parameters to skip fastqc and haplocheck (`--skip_fastqc` and `--skip_haplocheck`) [#438](https://github.com/nf-core/raredisease/pull/438) - CNVnator for copy number variant calling [#438](https://github.com/nf-core/raredisease/pull/434) - A new parameter `svdb_query_bedpedbs` to provide bedpe files as databases for SVDB query [#449](https://github.com/nf-core/raredisease/pull/449) -- ngsbits samplegender to check sex [#453] (https://github.com/nf-core/raredisease/pull/453) -- New workflow for generating cgh files frm SV vcfs for interpretation in the CytosSure interpretation software. Turned off by default +- ngsbits samplegender to check sex [#453](https://github.com/nf-core/raredisease/pull/453) +- New workflow for generating cgh files frm SV vcfs for interpretation in the CytosSure interpretation software. Turned off by default [#456](https://github.com/nf-core/raredisease/pull/456/) ### `Changed` diff --git a/conf/modules/generate_cytosure_files.config b/conf/modules/generate_cytosure_files.config index e42717b1..d8b96859 100644 --- a/conf/modules/generate_cytosure_files.config +++ b/conf/modules/generate_cytosure_files.config @@ -28,7 +28,7 @@ process { ext.args = { [ "--samples ${meta.id}", '--output-type z', - "--exclude 'clingen_ngiAF > 0.1 || swegenAF > 0.05 || clinical_genomics_mipAF > 0.05 || decipherAF > 0.1 || clinical_genomics_loqusFrq > 0.05 || gnomad_svAF > 0.05'" + "--exclude 'gnomad_svAF > 0.05'" ].join(' ') } ext.prefix = { ${meta.id} } } diff --git a/nextflow_schema.json b/nextflow_schema.json index 6429fd88..ed0f7fd0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -326,7 +326,8 @@ "help_text": "Optional file to blacklist regions for VCF2cytosure", "pattern": "^\\S+\\.bed$", "format": "file-path", - "fa_icon": "fas fa-file" + "fa_icon": "fas fa-file", + "description": "Path to vcf2cytosure blacklist file" }, "vcfanno_resources": { "type": "string", @@ -452,7 +453,6 @@ }, "vcf2cytosure_switch": { "type": "boolean", - "default": false, "description": "Turn on the vcf2cytosure subworkflow", "fa_icon": "fas fa-toggle-on", "help_text": "vcf2cytosure can generate CGH files from a structural variant VCF file that can be analysed in the CytoSure interpretation software. Cut offs for allele frequencies and bin sizes can be modified in the config file. Turned off by default." From 1a283f77a5611219dd6d07a4cc123ebd3c594a7c Mon Sep 17 00:00:00 2001 From: lucpen Date: Mon, 18 Dec 2023 16:30:17 +0100 Subject: [PATCH 174/387] fix run prettier --- CITATIONS.md | 1 + docs/usage.md | 2 +- 2 files changed, 2 insertions(+), 1 deletion(-) diff --git a/CITATIONS.md b/CITATIONS.md index c6252716..a302993e 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -49,6 +49,7 @@ > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. - [Fastp](https://github.com/OpenGene/fastp) + > Shifu, C. (2023). Ultrafast one-pass FASTQ data preprocessing, quality control, and deduplication using fastp. iMeta 2: e107. https://doi.org/10.1002/imt2.107 - [GATK](https://genome.cshlp.org/content/20/9/1297) diff --git a/docs/usage.md b/docs/usage.md index 5e3b7fd2..b4b500ce 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -151,7 +151,7 @@ The mandatory and optional parameters for each category are tabulated below. ##### 1. Alignment -| Mandatory | Optional | +| Mandatory | Optional | | ------------------------------ | ------------------------------ | | aligner1 | fasta_fai3 | | fasta | bwamem23 | From 1157f9649ade65df271c7de148d7119d26eca9aa Mon Sep 17 00:00:00 2001 From: lucpen Date: Mon, 18 Dec 2023 16:34:29 +0100 Subject: [PATCH 175/387] fix white space --- conf/modules/align.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules/align.config b/conf/modules/align.config index eb19e793..b3836566 100644 --- a/conf/modules/align.config +++ b/conf/modules/align.config @@ -28,7 +28,7 @@ process{ publishDir = [ path: { "${params.outdir}/trimming" }, mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } } From 36db2c7b757dec7373bef3bbd32e3a72d59aaeee Mon Sep 17 00:00:00 2001 From: jemten Date: Mon, 18 Dec 2023 16:34:33 +0100 Subject: [PATCH 176/387] updating citations --- CITATIONS.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/CITATIONS.md b/CITATIONS.md index 71414414..7dfc712e 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -130,6 +130,8 @@ > Kent WJ, Zweig AS, Barber G, Hinrichs AS, Karolchik D. BigWig and BigBed: enabling browsing of large distributed datasets. Bioinformatics. 2010;26(17):2204-2207. doi:10.1093/bioinformatics/btq351 +- [vcf2cytosure](https://github.com/NBISweden/vcf2cytosure) + - [Vcfanno](https://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-0973-5) > Pedersen BS, Layer RM, Quinlan AR. Vcfanno: fast, flexible annotation of genetic variants. Genome Biol. 2016;17(1):118. doi:10.1186/s13059-016-0973-5 From 3d12274b5104274128e8a19b9ba77058d5d86896 Mon Sep 17 00:00:00 2001 From: Anders Jemt Date: Mon, 18 Dec 2023 17:02:04 +0100 Subject: [PATCH 177/387] Update CHANGELOG.md MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Lucía Peña-Pérez --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index bfd2c315..47bbe9c1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -17,7 +17,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - CNVnator for copy number variant calling [#438](https://github.com/nf-core/raredisease/pull/434) - A new parameter `svdb_query_bedpedbs` to provide bedpe files as databases for SVDB query [#449](https://github.com/nf-core/raredisease/pull/449) - ngsbits samplegender to check sex [#453](https://github.com/nf-core/raredisease/pull/453) -- New workflow for generating cgh files frm SV vcfs for interpretation in the CytosSure interpretation software. Turned off by default [#456](https://github.com/nf-core/raredisease/pull/456/) +- New workflow for generating cgh files from SV vcfs for interpretation in the CytosSure interpretation software. Turned off by default [#456](https://github.com/nf-core/raredisease/pull/456/) ### `Changed` From e701fe267f2f9fd68ab4605c7b50fe6e86c36cfc Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Luc=C3=ADa=20Pe=C3=B1a-P=C3=A9rez?= Date: Mon, 18 Dec 2023 17:42:46 +0100 Subject: [PATCH 178/387] Apply suggestions from code review Co-authored-by: Anders Jemt --- CHANGELOG.md | 2 +- conf/modules/align.config | 4 ++-- nextflow.config | 4 ++-- nextflow_schema.json | 4 ++-- subworkflows/local/align.nf | 6 ++---- 5 files changed, 9 insertions(+), 11 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 08967e15..91d9c17c 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -16,7 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New parameters to skip fastqc and haplocheck (`--skip_fastqc` and `--skip_haplocheck`) [#438](https://github.com/nf-core/raredisease/pull/438) - CNVnator for copy number variant calling [#438](https://github.com/nf-core/raredisease/pull/434) - A new parameter `svdb_query_bedpedbs` to provide bedpe files as databases for SVDB query [#449](https://github.com/nf-core/raredisease/pull/449) -- Fastp to do adapter trimming. It can be skipped using`--skip_fastp`[#457](https://github.com/nf-core/raredisease/pull/457) +- Fastp to do adapter trimming. It can be skipped using `--skip_fastp` [#457](https://github.com/nf-core/raredisease/pull/457) - ngsbits samplegender to check sex [#453] (https://github.com/nf-core/raredisease/pull/453) ### `Changed` diff --git a/conf/modules/align.config b/conf/modules/align.config index b3836566..29fda646 100644 --- a/conf/modules/align.config +++ b/conf/modules/align.config @@ -20,11 +20,11 @@ process{ if (!params.skip_fastp) { withName: '.*ALIGN:FASTP' { - ext.args = [ + ext.args = { [ params.min_trimmed_length ? "--length_required ${params.min_trimmed_length}" : '', '--correction', '--overrepresentation_analysis' - ].join(' ').trim() + ].join(' ').trim() } publishDir = [ path: { "${params.outdir}/trimming" }, mode: params.publish_dir_mode, diff --git a/nextflow.config b/nextflow.config index 7c9a83b9..acc045de 100644 --- a/nextflow.config +++ b/nextflow.config @@ -47,7 +47,7 @@ params { // Alignment aligner = 'bwamem2' - min_trimmed_length = + min_trimmed_length = 40 rmdup = false // Variant calling @@ -55,7 +55,7 @@ params { variant_caller = 'deepvariant' // sentieon Defaults - ml_model = 40 + ml_model = '' // Dnascope SNV calling sentieon_dnascope_pcr_indel_model = 'CONSERVATIVE' diff --git a/nextflow_schema.json b/nextflow_schema.json index bb96cd29..9d268f2d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -52,8 +52,8 @@ "description": "Reference genome related files and options required for the workflow.", "properties": { "bait_padding": { - "type": "number", - "default": 100.0, + "type": "integer", + "default": 100, "fa_icon": "fas fa-align-center", "pattern": "^\\S+\\.bed(\\.gz)?$", "description": "The amount to pad each end of the target intervals to create bait intervals." diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index fa819926..e3c21b5b 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -32,14 +32,12 @@ workflow ALIGN { ch_bwamem2_bai = Channel.empty() ch_sentieon_bai = Channel.empty() - if (!params.skip_germlinecnvcaller) { - FASTP(ch_reads, [], false, false) + if (!params.skip_fastp) { + FASTP (ch_reads, [], false, false) ch_input_reads = FASTP.out.reads ch_versions = ch_versions.mix(FASTP.out.versions) } else { ch_input_reads = ch_reads } - //ch_input_reads.view() - if (params.aligner.equals("bwamem2")) { ALIGN_BWAMEM2 ( // Triggered when params.aligner is set as bwamem2 ch_input_reads, From 06b660667af9589e129369db88e83eef6d332e81 Mon Sep 17 00:00:00 2001 From: lucpen Date: Mon, 18 Dec 2023 18:19:12 +0100 Subject: [PATCH 179/387] feat added reviewers suggestion --- subworkflows/local/align.nf | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index e3c21b5b..07f5911d 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -34,13 +34,13 @@ workflow ALIGN { if (!params.skip_fastp) { FASTP (ch_reads, [], false, false) - ch_input_reads = FASTP.out.reads + ch_reads = FASTP.out.reads ch_versions = ch_versions.mix(FASTP.out.versions) - } else { ch_input_reads = ch_reads } + } if (params.aligner.equals("bwamem2")) { ALIGN_BWAMEM2 ( // Triggered when params.aligner is set as bwamem2 - ch_input_reads, + ch_reads, ch_genome_bwamem2index, ch_genome_fasta, ch_genome_fai, @@ -51,7 +51,7 @@ workflow ALIGN { ch_versions = ch_versions.mix(ALIGN_BWAMEM2.out.versions) } else if (params.aligner.equals("sentieon")) { ALIGN_SENTIEON ( // Triggered when params.aligner is set as sentieon - ch_input_reads, + ch_reads, ch_genome_fasta, ch_genome_fai, ch_genome_bwaindex, From 4be3219f85c77405f217429834011a2a858934f1 Mon Sep 17 00:00:00 2001 From: lucpen Date: Mon, 18 Dec 2023 20:37:50 +0100 Subject: [PATCH 180/387] feat add json to output --- subworkflows/local/align.nf | 5 ++++- 1 file changed, 4 insertions(+), 1 deletion(-) diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index 07f5911d..e7240f2c 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -27,6 +27,7 @@ workflow ALIGN { main: ch_versions = Channel.empty() + ch_fastp_json = Channel.empty() ch_bwamem2_bam = Channel.empty() ch_sentieon_bam = Channel.empty() ch_bwamem2_bai = Channel.empty() @@ -35,7 +36,8 @@ workflow ALIGN { if (!params.skip_fastp) { FASTP (ch_reads, [], false, false) ch_reads = FASTP.out.reads - ch_versions = ch_versions.mix(FASTP.out.versions) + ch_versions = ch_versions.mix(FASTP.out.versions) + ch_fastp_json = FASTP.out.json } if (params.aligner.equals("bwamem2")) { @@ -111,6 +113,7 @@ workflow ALIGN { CONVERT_MT_BAM_TO_FASTQ.out.versions) emit: + fastp_json = ch_fastp_json // channel: [ val(meta), path(json) ] genome_marked_bam = ch_genome_marked_bam // channel: [ val(meta), path(bam) ] genome_marked_bai = ch_genome_marked_bai // channel: [ val(meta), path(bai) ] genome_bam_bai = ch_genome_bam_bai // channel: [ val(meta), path(bam), path(bai) ] From 3027fb445f86454e3ee0b2c1fcb10705bf5b2e5f Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 19 Dec 2023 18:20:34 +0100 Subject: [PATCH 181/387] applying suggestion from code review --- conf/modules/generate_cytosure_files.config | 2 +- modules/nf-core/tiddit/cov/main.nf | 2 +- modules/nf-core/tiddit/cov/meta.yml | 2 +- modules/nf-core/vcf2cytosure/main.nf | 2 +- modules/nf-core/vcf2cytosure/vcf2cytosure.diff | 14 -------------- nextflow.config | 2 +- nextflow_schema.json | 13 +++++++------ subworkflows/local/generate_cytosure_files.nf | 6 +++--- workflows/raredisease.nf | 2 +- 9 files changed, 16 insertions(+), 29 deletions(-) delete mode 100644 modules/nf-core/vcf2cytosure/vcf2cytosure.diff diff --git a/conf/modules/generate_cytosure_files.config b/conf/modules/generate_cytosure_files.config index d8b96859..a1600d97 100644 --- a/conf/modules/generate_cytosure_files.config +++ b/conf/modules/generate_cytosure_files.config @@ -17,7 +17,7 @@ process { - if ( params.analysis_type != "wes" && params.vcf2cytosure_switch ) { + if ( params.analysis_type != "wes" && !params.skip_vcf2cytosure ) { withName: '.*GENERATE_CYTOSURE_FILES:TIDDIT_COV_VCF2CYTOSURE' { ext.args = { '-z 500' } diff --git a/modules/nf-core/tiddit/cov/main.nf b/modules/nf-core/tiddit/cov/main.nf index f4b1d675..e8cafaa5 100644 --- a/modules/nf-core/tiddit/cov/main.nf +++ b/modules/nf-core/tiddit/cov/main.nf @@ -41,7 +41,7 @@ process TIDDIT_COV { def prefix = task.ext.prefix ?: "${meta.id}" """ touch ${prefix}.wig - touch ${prefix}.tab + touch ${prefix}.bed cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/tiddit/cov/meta.yml b/modules/nf-core/tiddit/cov/meta.yml index 10171166..9cc586a9 100644 --- a/modules/nf-core/tiddit/cov/meta.yml +++ b/modules/nf-core/tiddit/cov/meta.yml @@ -44,7 +44,7 @@ output: - cov: type: file description: The coverage of different regions. Optional. - pattern: "*.tab" + pattern: "*.bed" - wig: type: file description: The coverage of different regions in WIG format. Optional. diff --git a/modules/nf-core/vcf2cytosure/main.nf b/modules/nf-core/vcf2cytosure/main.nf index f253f979..92351337 100644 --- a/modules/nf-core/vcf2cytosure/main.nf +++ b/modules/nf-core/vcf2cytosure/main.nf @@ -56,7 +56,7 @@ process VCF2CYTOSURE { def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${prefix}.xml + touch ${prefix}.cgh cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/vcf2cytosure/vcf2cytosure.diff b/modules/nf-core/vcf2cytosure/vcf2cytosure.diff deleted file mode 100644 index 660dfca7..00000000 --- a/modules/nf-core/vcf2cytosure/vcf2cytosure.diff +++ /dev/null @@ -1,14 +0,0 @@ -Changes in module 'nf-core/vcf2cytosure' ---- modules/nf-core/vcf2cytosure/main.nf -+++ modules/nf-core/vcf2cytosure/main.nf -@@ -12,7 +12,7 @@ - tuple val(meta2), path(coverage_bed) - tuple val(meta3), path(cns) - tuple val(meta4), path(snv_vcf) -- path blacklist_bed -+ path(blacklist_bed) - - output: - tuple val(meta), path("*.cgh"), emit: cgh - -************************************************************ diff --git a/nextflow.config b/nextflow.config index f2a67666..988c3c67 100644 --- a/nextflow.config +++ b/nextflow.config @@ -32,11 +32,11 @@ params { skip_snv_annotation = false skip_sv_annotation = false skip_mt_annotation = false + skip_vcf2cytosure = true skip_vep_filter = false gens_switch = false cadd_resources = null platform = 'illumina' - vcf2cytosure_switch = false // Bam_qc ngsbits_samplegender_method = 'xy' diff --git a/nextflow_schema.json b/nextflow_schema.json index ed0f7fd0..faca90b9 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -446,16 +446,17 @@ "description": "Specifies whether or not to skip annotate structural variant subworkflow.", "fa_icon": "fas fa-toggle-on" }, - "skip_vep_filter": { + "skip_vcf2cytosure": { "type": "boolean", - "description": "Specifies whether or not to filter results based on a list of candidate genes specified in 'vep_filters'.", + "default": true, + "description": "Specifies whether or not to skip the vcf2cytosure subworkflow", + "help_text": "vcf2cytosure can generate CGH files from a structural variant VCF file that can be analysed in the CytoSure interpretation software. Cut-offs for allele frequencies and bin sizes can be modified in the config file. Turned off by default.", "fa_icon": "fas fa-toggle-on" }, - "vcf2cytosure_switch": { + "skip_vep_filter": { "type": "boolean", - "description": "Turn on the vcf2cytosure subworkflow", - "fa_icon": "fas fa-toggle-on", - "help_text": "vcf2cytosure can generate CGH files from a structural variant VCF file that can be analysed in the CytoSure interpretation software. Cut offs for allele frequencies and bin sizes can be modified in the config file. Turned off by default." + "description": "Specifies whether or not to filter results based on a list of candidate genes specified in 'vep_filters'.", + "fa_icon": "fas fa-toggle-on" } } }, diff --git a/subworkflows/local/generate_cytosure_files.nf b/subworkflows/local/generate_cytosure_files.nf index c5b4f205..3d716f92 100644 --- a/subworkflows/local/generate_cytosure_files.nf +++ b/subworkflows/local/generate_cytosure_files.nf @@ -8,8 +8,8 @@ include { VCF2CYTOSURE } from '../../modules/nf-core workflow GENERATE_CYTOSURE_FILES { take: - ch_vcf // channel: [mandatory] [ val(meta), path(vcf), path(vcf_index) ] - ch_bam // channel: [mandatory] [ val(meta), path(bam) ] + ch_vcf // channel: [mandatory] [ val(meta), path(vcf), path(vcf_index) ] + ch_bam // channel: [mandatory] [ val(meta), path(bam) ] ch_blacklist // channel: [optional] [path(blacklist)] main: @@ -29,7 +29,7 @@ workflow GENERATE_CYTOSURE_FILES { VCF2CYTOSURE ( SPLIT_AND_FILTER_SV_VCF.out.vcf, TIDDIT_COV_VCF2CYTOSURE.out.cov, - [[], []], [[], []], + [[:], []], [[:], []], ch_blacklist ) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 4293d0b6..c1b12cd4 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -570,7 +570,7 @@ workflow RAREDISEASE { ch_versions = ch_versions.mix(PEDDY_CHECK.out.versions) // Generate CGH files from sequencing data, turned off by default - if ( params.vcf2cytosure_switch && params.analysis_type != "wes" ) { + if ( !params.skip_vcf2cytosure && params.analysis_type != "wes" ) { GENERATE_CYTOSURE_FILES ( BGZIPTABIX_SV.out.gz_tbi, ch_mapped.genome_marked_bam, From 2acb23447bb22d5ea8b0de299fad7d72fed16795 Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 19 Dec 2023 18:28:16 +0100 Subject: [PATCH 182/387] adding patch files --- modules.json | 3 ++- modules/nf-core/tiddit/cov/tiddit-cov.diff | 26 +++++++++++++++++++ .../nf-core/vcf2cytosure/vcf2cytosure.diff | 23 ++++++++++++++++ 3 files changed, 51 insertions(+), 1 deletion(-) create mode 100644 modules/nf-core/tiddit/cov/tiddit-cov.diff create mode 100644 modules/nf-core/vcf2cytosure/vcf2cytosure.diff diff --git a/modules.json b/modules.json index cb2c5a2e..8cfbb496 100644 --- a/modules.json +++ b/modules.json @@ -450,7 +450,8 @@ "tiddit/cov": { "branch": "master", "git_sha": "1c90a501d102b800c27697f5ef39a6e217ab1915", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/tiddit/cov/tiddit-cov.diff" }, "tiddit/sv": { "branch": "master", diff --git a/modules/nf-core/tiddit/cov/tiddit-cov.diff b/modules/nf-core/tiddit/cov/tiddit-cov.diff new file mode 100644 index 00000000..acf8216f --- /dev/null +++ b/modules/nf-core/tiddit/cov/tiddit-cov.diff @@ -0,0 +1,26 @@ +Changes in module 'nf-core/tiddit/cov' +--- modules/nf-core/tiddit/cov/meta.yml ++++ modules/nf-core/tiddit/cov/meta.yml +@@ -44,7 +44,7 @@ + - cov: + type: file + description: The coverage of different regions. Optional. +- pattern: "*.tab" ++ pattern: "*.bed" + - wig: + type: file + description: The coverage of different regions in WIG format. Optional. + +--- modules/nf-core/tiddit/cov/main.nf ++++ modules/nf-core/tiddit/cov/main.nf +@@ -41,7 +41,7 @@ + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.wig +- touch ${prefix}.tab ++ touch ${prefix}.bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + +************************************************************ diff --git a/modules/nf-core/vcf2cytosure/vcf2cytosure.diff b/modules/nf-core/vcf2cytosure/vcf2cytosure.diff new file mode 100644 index 00000000..f80980da --- /dev/null +++ b/modules/nf-core/vcf2cytosure/vcf2cytosure.diff @@ -0,0 +1,23 @@ +Changes in module 'nf-core/vcf2cytosure' +--- modules/nf-core/vcf2cytosure/main.nf ++++ modules/nf-core/vcf2cytosure/main.nf +@@ -12,7 +12,7 @@ + tuple val(meta2), path(coverage_bed) + tuple val(meta3), path(cns) + tuple val(meta4), path(snv_vcf) +- path blacklist_bed ++ path(blacklist_bed) + + output: + tuple val(meta), path("*.cgh"), emit: cgh +@@ -56,7 +56,7 @@ + def prefix = task.ext.prefix ?: "${meta.id}" + + """ +- touch ${prefix}.xml ++ touch ${prefix}.cgh + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + +************************************************************ From e5a3470bd832030fc0dfb35978322d77c9334bbe Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 19 Dec 2023 19:45:46 +0000 Subject: [PATCH 183/387] Template update for nf-core/tools version 2.11 --- .github/CONTRIBUTING.md | 3 + .github/PULL_REQUEST_TEMPLATE.md | 1 + .github/workflows/ci.yml | 2 +- .github/workflows/fix-linting.yml | 4 +- .github/workflows/linting.yml | 12 +- .gitpod.yml | 4 +- README.md | 17 +-- assets/multiqc_config.yml | 2 +- assets/slackreport.json | 2 +- conf/modules.config | 2 +- docs/usage.md | 4 +- lib/NfcoreTemplate.groovy | 32 ++--- modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 7 ++ .../custom/dumpsoftwareversions/main.nf | 6 +- .../custom/dumpsoftwareversions/meta.yml | 7 +- .../dumpsoftwareversions/tests/main.nf.test | 38 ++++++ .../tests/main.nf.test.snap | 27 +++++ .../dumpsoftwareversions/tests/tags.yml | 2 + modules/nf-core/fastqc/environment.yml | 7 ++ modules/nf-core/fastqc/main.nf | 10 +- modules/nf-core/fastqc/meta.yml | 5 + modules/nf-core/fastqc/tests/main.nf.test | 109 ++++++++++++++++++ .../nf-core/fastqc/tests/main.nf.test.snap | 10 ++ modules/nf-core/fastqc/tests/tags.yml | 2 + modules/nf-core/multiqc/environment.yml | 7 ++ modules/nf-core/multiqc/main.nf | 8 +- modules/nf-core/multiqc/meta.yml | 11 +- modules/nf-core/multiqc/tests/main.nf.test | 63 ++++++++++ modules/nf-core/multiqc/tests/tags.yml | 2 + nextflow.config | 10 +- 31 files changed, 358 insertions(+), 64 deletions(-) create mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml create mode 100644 modules/nf-core/fastqc/environment.yml create mode 100644 modules/nf-core/fastqc/tests/main.nf.test create mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastqc/tests/tags.yml create mode 100644 modules/nf-core/multiqc/environment.yml create mode 100644 modules/nf-core/multiqc/tests/main.nf.test create mode 100644 modules/nf-core/multiqc/tests/tags.yml diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index dc147015..39013956 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -27,6 +27,9 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests +You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to +receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. + When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 57aa63e7..fc4dc8ad 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -19,6 +19,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rare - [ ] If necessary, also make a PR on the nf-core/raredisease _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. - [ ] `CHANGELOG.md` is updated. diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 557706b3..612fc2fb 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,7 +28,7 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 831e96ca..b7ece6b1 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -13,7 +13,7 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 with: token: ${{ secrets.nf_core_bot_auth_token }} @@ -24,7 +24,7 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install Prettier run: npm install -g prettier @prettier/plugin-php diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index b8bdd214..905c58e4 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,9 +14,9 @@ jobs: EditorConfig: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install editorconfig-checker run: npm install -g editorconfig-checker @@ -27,9 +27,9 @@ jobs: Prettier: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install Prettier run: npm install -g prettier @@ -40,7 +40,7 @@ jobs: PythonBlack: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - name: Check code lints with Black uses: psf/black@stable @@ -71,7 +71,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 diff --git a/.gitpod.yml b/.gitpod.yml index 25488dcc..acf72695 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -4,7 +4,9 @@ tasks: command: | pre-commit install --install-hooks nextflow self-update - + - name: unset JAVA_TOOL_OPTIONS + command: | + unset JAVA_TOOL_OPTIONS vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/README.md b/README.md index bad14cdf..0eb4e6d5 100644 --- a/README.md +++ b/README.md @@ -30,11 +30,8 @@ ## Usage -:::note -If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how -to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) -with `-profile test` before running the workflow on actual data. -::: +> [!NOTE] +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. - + diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 069826a9..14de2020 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,5 +1,5 @@ report_comment: > - This report has been generated by the nf-core/raredisease + This report has been generated by the nf-core/raredisease analysis pipeline. For information about how to interpret these results, please see the documentation. report_section_order: diff --git a/assets/slackreport.json b/assets/slackreport.json index 73c55ea7..84f87e9b 100644 --- a/assets/slackreport.json +++ b/assets/slackreport.json @@ -3,7 +3,7 @@ { "fallback": "Plain-text summary of the attachment.", "color": "<% if (success) { %>good<% } else { %>danger<%} %>", - "author_name": "nf-core/raredisease v${version} - ${runName}", + "author_name": "nf-core/raredisease ${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/conf/modules.config b/conf/modules.config index 39e81386..d91c6aba 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -39,7 +39,7 @@ process { } withName: 'MULTIQC' { - ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' + ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ path: { "${params.outdir}/multiqc" }, mode: params.publish_dir_mode, diff --git a/docs/usage.md b/docs/usage.md index 74744c74..e710e34e 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -20,7 +20,7 @@ You will need to create a samplesheet with information about the samples you wou The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz @@ -33,7 +33,7 @@ The pipeline will auto-detect whether a sample is single- or paired-end using th A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index 01b8653d..e248e4c3 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -4,6 +4,7 @@ import org.yaml.snakeyaml.Yaml import groovy.json.JsonOutput +import nextflow.extension.FilesEx class NfcoreTemplate { @@ -141,12 +142,14 @@ class NfcoreTemplate { try { if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } [ 'sendmail', '-t' ].execute() << sendmail_html log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" } catch (all) { // Catch failures and try with plaintext def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { mail_cmd += [ '-A', mqc_report ] } mail_cmd.execute() << email_html @@ -155,14 +158,16 @@ class NfcoreTemplate { } // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() } // @@ -227,15 +232,14 @@ class NfcoreTemplate { // Dump pipeline parameters in a json file // public static void dump_parameters(workflow, params) { - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def output_pf = new File(output_d, "params_${timestamp}.json") + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") def jsonStr = JsonOutput.toJson(params) - output_pf.text = JsonOutput.prettyPrint(jsonStr) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() } // diff --git a/modules.json b/modules.json index 65847175..9b750add 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "bd8092b67b5103bdd52e300f75889442275c3117", + "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "4ab13872435962dadc239979554d13709e20bf29", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml new file mode 100644 index 00000000..f0c63f69 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -0,0 +1,7 @@ +name: custom_dumpsoftwareversions +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.17 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index ebc87273..7685b33c 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : + 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml index c32657de..5f15a5fd 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -1,4 +1,4 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: custom_dumpsoftwareversions description: Custom module used to dump software versions within the nf-core pipeline template keywords: @@ -16,7 +16,6 @@ input: type: file description: YML file containing software versions pattern: "*.yml" - output: - yml: type: file @@ -30,7 +29,9 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@drpatelh" - "@grst" +maintainers: + - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test new file mode 100644 index 00000000..eec1db10 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -0,0 +1,38 @@ +nextflow_process { + + name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" + script "../main.nf" + process "CUSTOM_DUMPSOFTWAREVERSIONS" + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "dumpsoftwareversions" + tag "custom/dumpsoftwareversions" + + test("Should run without failures") { + when { + process { + """ + def tool1_version = ''' + TOOL1: + tool1: 0.11.9 + '''.stripIndent() + + def tool2_version = ''' + TOOL2: + tool2: 1.9 + '''.stripIndent() + + input[0] = Channel.of(tool1_version, tool2_version).collectFile() + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap new file mode 100644 index 00000000..4274ed57 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -0,0 +1,27 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ], + "1": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "2": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "mqc_yml": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "versions": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "yml": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ] + } + ], + "timestamp": "2023-11-03T14:43:22.157011" + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml new file mode 100644 index 00000000..405aa24a --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml @@ -0,0 +1,2 @@ +custom/dumpsoftwareversions: + - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml new file mode 100644 index 00000000..1787b38a --- /dev/null +++ b/modules/nf-core/fastqc/environment.yml @@ -0,0 +1,7 @@ +name: fastqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 249f9064..9e19a74c 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -2,10 +2,10 @@ process FASTQC { tag "$meta.id" label 'process_medium' - conda "bioconda::fastqc=0.11.9" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' : - 'biocontainers/fastqc:0.11.9--0' }" + 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : + 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" input: tuple val(meta), path(reads) @@ -37,7 +37,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ @@ -49,7 +49,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ } diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml index 4da5bb5a..ee5507e0 100644 --- a/modules/nf-core/fastqc/meta.yml +++ b/modules/nf-core/fastqc/meta.yml @@ -50,3 +50,8 @@ authors: - "@grst" - "@ewels" - "@FelixKrueger" +maintainers: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test new file mode 100644 index 00000000..b9e8f926 --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -0,0 +1,109 @@ +nextflow_process { + + name "Test Process FASTQC" + script "../main.nf" + process "FASTQC" + tag "modules" + tag "modules_nfcore" + tag "fastqc" + + test("Single-Read") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id: 'test', single_end:true ], + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:
Mon 2 Oct 2023
test.gz
+ // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, + { assert path(process.out.html.get(0).get(1)).getText().contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + ) + } + } +// TODO +// // +// // Test with paired-end data +// // +// workflow test_fastqc_paired_end { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with interleaved data +// // +// workflow test_fastqc_interleaved { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with bam data +// // +// workflow test_fastqc_bam { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with multiple samples +// // +// workflow test_fastqc_multiple { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with custom prefix +// // +// workflow test_fastqc_custom_prefix { +// input = [ +// [ id:'mysample', single_end:true ], // meta map +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } +} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap new file mode 100644 index 00000000..636a32ce --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -0,0 +1,10 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2023-10-09T23:40:54+0000" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml new file mode 100644 index 00000000..7834294b --- /dev/null +++ b/modules/nf-core/fastqc/tests/tags.yml @@ -0,0 +1,2 @@ +fastqc: + - modules/nf-core/fastqc/** diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml new file mode 100644 index 00000000..bc0bdb5b --- /dev/null +++ b/modules/nf-core/multiqc/environment.yml @@ -0,0 +1,7 @@ +name: multiqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.18 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1fc387be..00cc48d2 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -1,10 +1,10 @@ process MULTIQC { label 'process_single' - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : + 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -25,12 +25,14 @@ process MULTIQC { def args = task.ext.args ?: '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' + def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' """ multiqc \\ --force \\ $args \\ $config \\ $extra_config \\ + $logo \\ . cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f93b5ee5..f1aa660e 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,5 +1,5 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json -name: MultiQC +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: - QC @@ -13,7 +13,6 @@ tools: homepage: https://multiqc.info/ documentation: https://multiqc.info/docs/ licence: ["GPL-3.0-or-later"] - input: - multiqc_files: type: file @@ -31,7 +30,6 @@ input: type: file description: Optional logo file for MultiQC pattern: "*.{png}" - output: - report: type: file @@ -54,3 +52,8 @@ authors: - "@bunop" - "@drpatelh" - "@jfy133" +maintainers: + - "@abhi18av" + - "@bunop" + - "@drpatelh" + - "@jfy133" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test new file mode 100644 index 00000000..c2dad217 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -0,0 +1,63 @@ +nextflow_process { + + name "Test Process MULTIQC" + script "../main.nf" + process "MULTIQC" + tag "modules" + tag "modules_nfcore" + tag "multiqc" + + test("MULTIQC: FASTQC") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } + + test("MULTIQC: FASTQC and a config file") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } +} diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml new file mode 100644 index 00000000..bea6c0d3 --- /dev/null +++ b/modules/nf-core/multiqc/tests/tags.yml @@ -0,0 +1,2 @@ +multiqc: + - modules/nf-core/multiqc/** diff --git a/nextflow.config b/nextflow.config index e4009f33..b1c0c8ff 100644 --- a/nextflow.config +++ b/nextflow.config @@ -14,7 +14,7 @@ params { input = null // References genome = null - igenomes_base = 's3://ngi-igenomes/igenomes' + igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false @@ -82,6 +82,7 @@ profiles { dumpHashes = true process.beforeScript = 'echo $HOSTNAME' cleanup = false + nextflow.enable.configProcessNamesValidation = true } conda { conda.enabled = true @@ -104,13 +105,13 @@ profiles { } docker { docker.enabled = true - docker.userEmulation = true conda.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false + runOptions = '-u $(id -u):$(id -g)' } arm { docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' @@ -181,7 +182,7 @@ singularity.registry = 'quay.io' // Nextflow plugins plugins { - id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet } // Load igenomes.config if required @@ -204,6 +205,9 @@ env { // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] +// Disable process selector warnings by default. Use debug profile to enable warnings. +nextflow.enable.configProcessNamesValidation = false + def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true From f5594e639331e75c320dab6db086d1a1228a4281 Mon Sep 17 00:00:00 2001 From: lucpen Date: Wed, 20 Dec 2023 13:27:51 +0100 Subject: [PATCH 184/387] feat added specific values for some samplesheet params --- assets/schema_input.json | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index 191f1394..cb881db8 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -39,15 +39,17 @@ ] }, "sex": { - "type": "string", + "type": "integer", "meta": ["sex"], "pattern": "^\\S+$", + "enum": [0, 1, 2], "errorMessage": "Sex must be provided and cannot contain spaces" }, "phenotype": { - "type": "string", + "type": "integer", "meta": ["phenotype"], "pattern": "^\\S+$", + "enum": [0, 1, 2], "errorMessage": "Phenotype must be provided and cannot contain spaces" }, "paternal_id": { From ffc108cc68038465019fb9c9d77528f555ee44da Mon Sep 17 00:00:00 2001 From: jemten Date: Wed, 20 Dec 2023 15:40:33 +0100 Subject: [PATCH 185/387] fixing modules discrepancies --- modules.json | 4 +- modules/nf-core/cat/cat/environment.yml | 7 + modules/nf-core/cat/cat/main.nf | 14 +- modules/nf-core/cat/cat/meta.yml | 7 +- modules/nf-core/cat/cat/tests/main.nf.test | 179 ++++++++++++++++++ .../nf-core/cat/cat/tests/main.nf.test.snap | 121 ++++++++++++ .../cat/tests/nextflow_unzipped_zipped.config | 6 + .../cat/tests/nextflow_zipped_unzipped.config | 8 + modules/nf-core/cat/cat/tests/tags.yml | 2 + .../templates/dumpsoftwareversions.py | 101 ++++++++++ 10 files changed, 440 insertions(+), 9 deletions(-) create mode 100644 modules/nf-core/cat/cat/environment.yml create mode 100644 modules/nf-core/cat/cat/tests/main.nf.test create mode 100644 modules/nf-core/cat/cat/tests/main.nf.test.snap create mode 100644 modules/nf-core/cat/cat/tests/nextflow_unzipped_zipped.config create mode 100644 modules/nf-core/cat/cat/tests/nextflow_zipped_unzipped.config create mode 100644 modules/nf-core/cat/cat/tests/tags.yml create mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py diff --git a/modules.json b/modules.json index 161faef3..e0a92fa6 100644 --- a/modules.json +++ b/modules.json @@ -72,7 +72,7 @@ }, "cat/cat": { "branch": "master", - "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", + "git_sha": "d593e8f6b7d1bbbb2acf43a4b9efeeac8d6720f2", "installed_by": ["modules"] }, "chromograph": { @@ -92,7 +92,7 @@ }, "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "05c280924b6c768d484c7c443dad5e605c4ff4b4", + "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", "installed_by": ["modules"] }, "deepvariant": { diff --git a/modules/nf-core/cat/cat/environment.yml b/modules/nf-core/cat/cat/environment.yml new file mode 100644 index 00000000..17a04ef2 --- /dev/null +++ b/modules/nf-core/cat/cat/environment.yml @@ -0,0 +1,7 @@ +name: cat_cat +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - conda-forge::pigz=2.3.4 diff --git a/modules/nf-core/cat/cat/main.nf b/modules/nf-core/cat/cat/main.nf index 9f062219..970ab760 100644 --- a/modules/nf-core/cat/cat/main.nf +++ b/modules/nf-core/cat/cat/main.nf @@ -2,7 +2,7 @@ process CAT_CAT { tag "$meta.id" label 'process_low' - conda "conda-forge::pigz=2.3.4" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/pigz:2.3.4' : 'biocontainers/pigz:2.3.4' }" @@ -35,6 +35,10 @@ process CAT_CAT { in_zip = file_list[0].endsWith('.gz') command1 = (in_zip && !out_zip) ? 'zcat' : 'cat' command2 = (!in_zip && out_zip) ? "| pigz -c -p $task.cpus $args2" : '' + if(file_list.contains(prefix.trim())) { + error "The name of the input file can't be the same as for the output prefix in the " + + "module CAT_CAT (currently `$prefix`). Please choose a different one." + } """ $command1 \\ $args \\ @@ -49,8 +53,12 @@ process CAT_CAT { """ stub: - def file_list = files_in.collect { it.toString() } - prefix = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}" + def file_list = files_in.collect { it.toString() } + prefix = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}" + if(file_list.contains(prefix.trim())) { + error "The name of the input file can't be the same as for the output prefix in the " + + "module CAT_CAT (currently `$prefix`). Please choose a different one." + } """ touch $prefix diff --git a/modules/nf-core/cat/cat/meta.yml b/modules/nf-core/cat/cat/meta.yml index 8acc0bfa..00a8db0b 100644 --- a/modules/nf-core/cat/cat/meta.yml +++ b/modules/nf-core/cat/cat/meta.yml @@ -7,9 +7,7 @@ keywords: tools: - cat: description: Just concatenation - documentation: https://man7.org/linux/man-pages/man1/cat.1.html - licence: ["GPL-3.0-or-later"] input: - meta: @@ -21,7 +19,6 @@ input: type: file description: List of compressed / uncompressed files pattern: "*" - output: - versions: type: file @@ -31,7 +28,9 @@ output: type: file description: Concatenated file. Will be gzipped if file_out ends with ".gz" pattern: "${file_out}" - authors: - "@erikrikarddaniel" - "@FriederikeHanssen" +maintainers: + - "@erikrikarddaniel" + - "@FriederikeHanssen" diff --git a/modules/nf-core/cat/cat/tests/main.nf.test b/modules/nf-core/cat/cat/tests/main.nf.test new file mode 100644 index 00000000..ed5a4f12 --- /dev/null +++ b/modules/nf-core/cat/cat/tests/main.nf.test @@ -0,0 +1,179 @@ +nextflow_process { + + name "Test Process CAT_CAT" + script "../main.nf" + process "CAT_CAT" + tag "modules" + tag "modules_nfcore" + tag "cat" + tag "cat/cat" + + test("test_cat_name_conflict") { + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'genome', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + ] + ] + """ + } + } + then { + assertAll( + { assert !process.success }, + { assert process.stdout.toString().contains("The name of the input file can't be the same as for the output prefix") } + ) + } + } + + test("test_cat_unzipped_unzipped") { + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + ] + ] + """ + } + } + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + + test("test_cat_zipped_zipped") { + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) + ] + ] + """ + } + } + then { + def lines = path(process.out.file_out.get(0).get(1)).linesGzip + assertAll( + { assert process.success }, + { assert snapshot(lines[0..5]).match("test_cat_zipped_zipped_lines") }, + { assert snapshot(lines.size()).match("test_cat_zipped_zipped_size")} + ) + } + } + + test("test_cat_zipped_unzipped") { + config './nextflow_zipped_unzipped.config' + + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("test_cat_unzipped_zipped") { + config './nextflow_unzipped_zipped.config' + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + ] + ] + """ + } + } + then { + def lines = path(process.out.file_out.get(0).get(1)).linesGzip + assertAll( + { assert process.success }, + { assert snapshot(lines[0..5]).match("test_cat_unzipped_zipped_lines") }, + { assert snapshot(lines.size()).match("test_cat_unzipped_zipped_size")} + ) + } + } + + test("test_cat_one_file_unzipped_zipped") { + config './nextflow_unzipped_zipped.config' + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + ] + """ + } + } + then { + def lines = path(process.out.file_out.get(0).get(1)).linesGzip + assertAll( + { assert process.success }, + { assert snapshot(lines[0..5]).match("test_cat_one_file_unzipped_zipped_lines") }, + { assert snapshot(lines.size()).match("test_cat_one_file_unzipped_zipped_size")} + ) + } + } +} + diff --git a/modules/nf-core/cat/cat/tests/main.nf.test.snap b/modules/nf-core/cat/cat/tests/main.nf.test.snap new file mode 100644 index 00000000..423571ba --- /dev/null +++ b/modules/nf-core/cat/cat/tests/main.nf.test.snap @@ -0,0 +1,121 @@ +{ + "test_cat_unzipped_zipped_size": { + "content": [ + 375 + ], + "timestamp": "2023-10-16T14:33:08.049445686" + }, + "test_cat_unzipped_unzipped": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.fasta:md5,f44b33a0e441ad58b2d3700270e2dbe2" + ] + ], + "1": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ], + "file_out": [ + [ + { + "id": "test", + "single_end": true + }, + "test.fasta:md5,f44b33a0e441ad58b2d3700270e2dbe2" + ] + ], + "versions": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ] + } + ], + "timestamp": "2023-10-16T14:32:18.500464399" + }, + "test_cat_zipped_unzipped": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "cat.txt:md5,c439d3b60e7bc03e8802a451a0d9a5d9" + ] + ], + "1": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ], + "file_out": [ + [ + { + "id": "test", + "single_end": true + }, + "cat.txt:md5,c439d3b60e7bc03e8802a451a0d9a5d9" + ] + ], + "versions": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ] + } + ], + "timestamp": "2023-10-16T14:32:49.642741302" + }, + "test_cat_zipped_zipped_lines": { + "content": [ + [ + "MT192765.1\tGenbank\ttranscript\t259\t29667\t.\t+\t.\tID=unknown_transcript_1;geneID=orf1ab;gene_name=orf1ab", + "MT192765.1\tGenbank\tgene\t259\t21548\t.\t+\t.\tParent=unknown_transcript_1", + "MT192765.1\tGenbank\tCDS\t259\t13461\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1", + "MT192765.1\tGenbank\tCDS\t13461\t21548\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1", + "MT192765.1\tGenbank\tCDS\t21556\t25377\t.\t+\t0\tParent=unknown_transcript_1;gbkey=CDS;gene=S;note=\"structural protein\";product=\"surface glycoprotein\";protein_id=QIK50427.1", + "MT192765.1\tGenbank\tgene\t21556\t25377\t.\t+\t.\tParent=unknown_transcript_1" + ] + ], + "timestamp": "2023-10-16T14:32:33.629048645" + }, + "test_cat_unzipped_zipped_lines": { + "content": [ + [ + ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome", + "GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGT", + "GTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAG", + "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG", + "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT", + "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG" + ] + ], + "timestamp": "2023-10-16T14:33:08.038830506" + }, + "test_cat_one_file_unzipped_zipped_lines": { + "content": [ + [ + ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome", + "GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGT", + "GTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAG", + "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG", + "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT", + "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG" + ] + ], + "timestamp": "2023-10-16T14:33:21.39642399" + }, + "test_cat_zipped_zipped_size": { + "content": [ + 78 + ], + "timestamp": "2023-10-16T14:32:33.641869244" + }, + "test_cat_one_file_unzipped_zipped_size": { + "content": [ + 374 + ], + "timestamp": "2023-10-16T14:33:21.4094373" + } +} \ No newline at end of file diff --git a/modules/nf-core/cat/cat/tests/nextflow_unzipped_zipped.config b/modules/nf-core/cat/cat/tests/nextflow_unzipped_zipped.config new file mode 100644 index 00000000..ec26b0fd --- /dev/null +++ b/modules/nf-core/cat/cat/tests/nextflow_unzipped_zipped.config @@ -0,0 +1,6 @@ + +process { + withName: CAT_CAT { + ext.prefix = 'cat.txt.gz' + } +} diff --git a/modules/nf-core/cat/cat/tests/nextflow_zipped_unzipped.config b/modules/nf-core/cat/cat/tests/nextflow_zipped_unzipped.config new file mode 100644 index 00000000..fbc79783 --- /dev/null +++ b/modules/nf-core/cat/cat/tests/nextflow_zipped_unzipped.config @@ -0,0 +1,8 @@ + +process { + + withName: CAT_CAT { + ext.prefix = 'cat.txt' + } + +} diff --git a/modules/nf-core/cat/cat/tests/tags.yml b/modules/nf-core/cat/cat/tests/tags.yml new file mode 100644 index 00000000..37b578f5 --- /dev/null +++ b/modules/nf-core/cat/cat/tests/tags.yml @@ -0,0 +1,2 @@ +cat/cat: + - modules/nf-core/cat/cat/** diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py new file mode 100755 index 00000000..da033408 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py @@ -0,0 +1,101 @@ +#!/usr/bin/env python + + +"""Provide functions to merge multiple versions.yml files.""" + + +import yaml +import platform +from textwrap import dedent + + +def _make_versions_html(versions): + """Generate a tabular HTML output of all versions for MultiQC.""" + html = [ + dedent( + """\\ + + + + + + + + + + """ + ) + ] + for process, tmp_versions in sorted(versions.items()): + html.append("") + for i, (tool, version) in enumerate(sorted(tmp_versions.items())): + html.append( + dedent( + f"""\\ + + + + + + """ + ) + ) + html.append("") + html.append("
Process Name Software Version
{process if (i == 0) else ''}{tool}{version}
") + return "\\n".join(html) + + +def main(): + """Load all version files and generate merged output.""" + versions_this_module = {} + versions_this_module["${task.process}"] = { + "python": platform.python_version(), + "yaml": yaml.__version__, + } + + with open("$versions") as f: + versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module + + # aggregate versions by the module name (derived from fully-qualified process name) + versions_by_module = {} + for process, process_versions in versions_by_process.items(): + module = process.split(":")[-1] + try: + if versions_by_module[module] != process_versions: + raise AssertionError( + "We assume that software versions are the same between all modules. " + "If you see this error-message it means you discovered an edge-case " + "and should open an issue in nf-core/tools. " + ) + except KeyError: + versions_by_module[module] = process_versions + + versions_by_module["Workflow"] = { + "Nextflow": "$workflow.nextflow.version", + "$workflow.manifest.name": "$workflow.manifest.version", + } + + versions_mqc = { + "id": "software_versions", + "section_name": "${workflow.manifest.name} Software Versions", + "section_href": "https://github.com/${workflow.manifest.name}", + "plot_type": "html", + "description": "are collected at run time from the software output.", + "data": _make_versions_html(versions_by_module), + } + + with open("software_versions.yml", "w") as f: + yaml.dump(versions_by_module, f, default_flow_style=False) + with open("software_versions_mqc.yml", "w") as f: + yaml.dump(versions_mqc, f, default_flow_style=False) + + with open("versions.yml", "w") as f: + yaml.dump(versions_this_module, f, default_flow_style=False) + + +if __name__ == "__main__": + main() From 8cee95440f7b5b135deda116978b2a7ecf8abd31 Mon Sep 17 00:00:00 2001 From: Jakob Willforss Date: Wed, 20 Dec 2023 15:47:59 +0100 Subject: [PATCH 186/387] Add flag to skip reference output from CNVcaller --- modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf index d916c2af..98652570 100644 --- a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf +++ b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf @@ -41,7 +41,8 @@ process GATK4_POSTPROCESSGERMLINECNVCALLS { $ploidy_command \\ --output-genotyped-intervals ${prefix}_genotyped_intervals.vcf.gz \\ --output-genotyped-segments ${prefix}_genotyped_segments.vcf.gz \\ - --output-denoised-copy-ratios ${prefix}_denoised.vcf.gz + --output-denoised-copy-ratios ${prefix}_denoised.vcf.gz \\ + --sites-only-vcf-output cat <<-END_VERSIONS > versions.yml "${task.process}": From be78df4c78daac959fd1f6794195f5f857111f10 Mon Sep 17 00:00:00 2001 From: Jakob Willforss Date: Wed, 20 Dec 2023 15:48:33 +0100 Subject: [PATCH 187/387] Use segments instead of intervals in GATK CNVCaller output --- subworkflows/local/call_structural_variants.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/call_structural_variants.nf b/subworkflows/local/call_structural_variants.nf index 13827f61..e16275c9 100644 --- a/subworkflows/local/call_structural_variants.nf +++ b/subworkflows/local/call_structural_variants.nf @@ -46,7 +46,7 @@ workflow CALL_STRUCTURAL_VARIANTS { if (!params.skip_germlinecnvcaller) { CALL_SV_GERMLINECNVCALLER (ch_genome_bam_bai, ch_genome_fasta, ch_genome_fai, ch_readcount_intervals, ch_genome_dictionary, ch_ploidy_model, ch_gcnvcaller_model) - .genotyped_intervals_vcf + .genotyped_segments_vcf .collect{it[1]} .set { gcnvcaller_vcf } From bcacf2506a55bcfd79333ff40db790044ff174c9 Mon Sep 17 00:00:00 2001 From: Jakob Willforss Date: Wed, 20 Dec 2023 15:49:24 +0100 Subject: [PATCH 188/387] Retrieve the correct value for GATK CNVCaller intervals --- .../local/variant_calling/call_sv_germlinecnvcaller.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf b/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf index 029c0db5..8852a1a2 100644 --- a/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf +++ b/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf @@ -12,7 +12,7 @@ workflow CALL_SV_GERMLINECNVCALLER { ch_bam_bai // channel: [mandatory][ val(meta), path(bam), path(bai) ] ch_fasta // channel: [mandatory][ val(meta), path(ch_fasta_no_meta) ] ch_fai // channel: [mandatory][ val(meta), path(ch_fai) ] - ch_readcount_intervals // channel: [mandatory][ val(meta), path(bed), path(tbi) ] + ch_readcount_intervals // channel: [mandatory][ path(intervals) ] ch_genome_dictionary // channel: [mandatory][ val(meta), path(ch_dict) ] ch_ploidy_model // channel: [mandatory][ path(ch_ploidy_model) ] ch_gcnvcaller_model // channel: [mandatory][ path(ch_gcnvcaller_model) ] @@ -20,7 +20,7 @@ workflow CALL_SV_GERMLINECNVCALLER { main: ch_versions = Channel.empty() - input = ch_bam_bai.combine( ch_readcount_intervals.collect{ it[1] } ) + input = ch_bam_bai.combine( ch_readcount_intervals ) GATK4_COLLECTREADCOUNTS ( input, ch_fasta, ch_fai, ch_genome_dictionary ) From d39df82fa1fca8d50eedd07007eebf87e3563cfc Mon Sep 17 00:00:00 2001 From: jemten Date: Wed, 20 Dec 2023 16:27:34 +0100 Subject: [PATCH 189/387] moving the github ci test to stub for one sample --- .github/workflows/ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index a97b039d..8d463ee6 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,7 +28,7 @@ jobs: - "latest-everything" parameters: - "-profile test,docker" - - "-profile test_one_sample,docker" + - "-profile test_one_sample,docker -stub" steps: - name: Check out pipeline code uses: actions/checkout@v4 From 03eb038f6641f46b9cfdacc0537712eb0058fa55 Mon Sep 17 00:00:00 2001 From: jemten Date: Wed, 20 Dec 2023 16:44:07 +0100 Subject: [PATCH 190/387] turning of fastp for github tests --- .github/workflows/ci.yml | 2 +- conf/test.config | 1 + conf/test_one_sample.config | 1 + 3 files changed, 3 insertions(+), 1 deletion(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 8d463ee6..a97b039d 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,7 +28,7 @@ jobs: - "latest-everything" parameters: - "-profile test,docker" - - "-profile test_one_sample,docker -stub" + - "-profile test_one_sample,docker" steps: - name: Check out pipeline code uses: actions/checkout@v4 diff --git a/conf/test.config b/conf/test.config index d398d542..af349e6b 100644 --- a/conf/test.config +++ b/conf/test.config @@ -25,6 +25,7 @@ params { // analysis params skip_eklipse = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_fastp = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_fastqc = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_germlinecnvcaller = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_haplocheck = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index f39cb40c..a2f42a80 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -25,6 +25,7 @@ params { // analysis params skip_eklipse = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_fastp = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_fastqc = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_germlinecnvcaller = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_haplocheck = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI From f0aa68acdc947e2ef9d827b00f447523dc993d29 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 20 Dec 2023 15:57:46 +0000 Subject: [PATCH 191/387] Template update for nf-core/tools version 2.11.1 --- .../{release-announcments.yml => release-announcements.yml} | 0 nextflow.config | 4 ++-- 2 files changed, 2 insertions(+), 2 deletions(-) rename .github/workflows/{release-announcments.yml => release-announcements.yml} (100%) diff --git a/.github/workflows/release-announcments.yml b/.github/workflows/release-announcements.yml similarity index 100% rename from .github/workflows/release-announcments.yml rename to .github/workflows/release-announcements.yml diff --git a/nextflow.config b/nextflow.config index b1c0c8ff..9e9e442b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -111,10 +111,10 @@ profiles { shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false - runOptions = '-u $(id -u):$(id -g)' + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { singularity.enabled = true From a9926bb806a88f2e56455f1871e8305a052d54fa Mon Sep 17 00:00:00 2001 From: jemten Date: Wed, 20 Dec 2023 17:18:55 +0100 Subject: [PATCH 192/387] moving fastp back in --- conf/test.config | 1 - conf/test_one_sample.config | 1 - 2 files changed, 2 deletions(-) diff --git a/conf/test.config b/conf/test.config index af349e6b..d398d542 100644 --- a/conf/test.config +++ b/conf/test.config @@ -25,7 +25,6 @@ params { // analysis params skip_eklipse = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI - skip_fastp = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_fastqc = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_germlinecnvcaller = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_haplocheck = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index a2f42a80..f39cb40c 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -25,7 +25,6 @@ params { // analysis params skip_eklipse = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI - skip_fastp = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_fastqc = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_germlinecnvcaller = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_haplocheck = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI From 82a96cb6484234f25c986e56c8d8ee1fdda107fa Mon Sep 17 00:00:00 2001 From: lucpen Date: Thu, 21 Dec 2023 11:21:33 +0100 Subject: [PATCH 193/387] fix integer to number --- workflows/raredisease.nf | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index c1b12cd4..c4183c93 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -686,16 +686,16 @@ def create_case_channel(List rows) { def mother = "" for (item in rows) { - if (item.phenotype == "2") { + if (item.phenotype == 2) { probands.add(item.sample) } - if ( (item.paternal!="0") && (item.paternal!="") && (item.maternal!="0") && (item.maternal!="") ) { + if ( (item.paternal!=0) && (item.paternal!="") && (item.maternal!=0) && (item.maternal!="") ) { upd_children.add(item.sample) } - if ( (item.paternal!="0") && (item.paternal!="") ) { + if ( (item.paternal!=0) && (item.paternal!="") ) { father = item.paternal } - if ( (item.maternal!="0") && (item.maternal!="") ) { + if ( (item.maternal!=0) && (item.maternal!="") ) { mother = item.maternal } } From 5a2c55c5aae2b0fd89e33737be13b09ab719259e Mon Sep 17 00:00:00 2001 From: lucpen Date: Fri, 22 Dec 2023 07:18:49 +0100 Subject: [PATCH 194/387] fix --- conf/modules/call_repeat_expansions.config | 2 +- conf/modules/generate_cytosure_files.config | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/modules/call_repeat_expansions.config b/conf/modules/call_repeat_expansions.config index 0509d1e2..13a7e519 100644 --- a/conf/modules/call_repeat_expansions.config +++ b/conf/modules/call_repeat_expansions.config @@ -24,7 +24,7 @@ process { } withName: '.*CALL_REPEAT_EXPANSIONS:EXPANSIONHUNTER' { - ext.args = { ("${meta.sex}" == '1') ? '--sex male' : '--sex female' } + ext.args = { ("${meta.sex}" == 1) ? '--sex male' : '--sex female' } ext.prefix = { "${meta.id}_exphunter" } } diff --git a/conf/modules/generate_cytosure_files.config b/conf/modules/generate_cytosure_files.config index a1600d97..323b64e3 100644 --- a/conf/modules/generate_cytosure_files.config +++ b/conf/modules/generate_cytosure_files.config @@ -35,7 +35,7 @@ process { withName: '.*GENERATE_CYTOSURE_FILES:VCF2CYTOSURE' { ext.args = { [ - meta.sex.equals('1') ? '--sex male' : '--sex female', + meta.sex.equals(1) ? '--sex male' : '--sex female', '--size 5000', '--maxbnd 5000' ].join(' ') } From f48e013ff44d639827abfc07a423173d5e615d75 Mon Sep 17 00:00:00 2001 From: Jakob Willforss Date: Fri, 22 Dec 2023 10:09:35 +0100 Subject: [PATCH 195/387] Suggested filtering of the POSTPROCESSGERMLINECNVCALLS --- .../variant_calling/call_sv_germlinecnvcaller.nf | 14 ++++++++++---- 1 file changed, 10 insertions(+), 4 deletions(-) diff --git a/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf b/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf index 8852a1a2..a9420a62 100644 --- a/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf +++ b/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf @@ -6,6 +6,7 @@ include { GATK4_COLLECTREADCOUNTS } from '../../../modules/nf-core/g include { GATK4_DETERMINEGERMLINECONTIGPLOIDY } from '../../../modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf' include { GATK4_GERMLINECNVCALLER } from '../../../modules/nf-core/gatk4/germlinecnvcaller/main.nf' include { GATK4_POSTPROCESSGERMLINECNVCALLS } from '../../../modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf' +include { BCFTOOLS_VIEW } from '../../../modules/nf-core/bcftools/view/main' workflow CALL_SV_GERMLINECNVCALLER { take: @@ -47,16 +48,21 @@ workflow CALL_SV_GERMLINECNVCALLER { GATK4_POSTPROCESSGERMLINECNVCALLS ( ch_postproc_in ) + // Filter out reference only (0/0) segments + BCFTOOLS_VIEW ( GATK4_POSTPROCESSGERMLINECNVCALLS.out.segments, [], [], [] ) + ch_versions = ch_versions.mix(GATK4_COLLECTREADCOUNTS.out.versions) ch_versions = ch_versions.mix(GATK4_DETERMINEGERMLINECONTIGPLOIDY.out.versions) ch_versions = ch_versions.mix(GATK4_GERMLINECNVCALLER.out.versions) ch_versions = ch_versions.mix(GATK4_POSTPROCESSGERMLINECNVCALLS.out.versions) + ch_versions = ch_versions.mix(BCFTOOLS_VIEW.out.versions) emit: - genotyped_intervals_vcf = GATK4_POSTPROCESSGERMLINECNVCALLS.out.intervals // channel: [ val(meta), path(*.tar.gz) ] - genotyped_segments_vcf = GATK4_POSTPROCESSGERMLINECNVCALLS.out.segments // channel: [ val(meta), path(*.tar.gz) ] - denoised_vcf = GATK4_POSTPROCESSGERMLINECNVCALLS.out.denoised // channel: [ val(meta), path(*.tar.gz) ] - versions = ch_versions // channel: [ versions.yml ] + genotyped_intervals_vcf = GATK4_POSTPROCESSGERMLINECNVCALLS.out.intervals // channel: [ val(meta), path(*.tar.gz) ] + genotyped_segments_vcf = GATK4_POSTPROCESSGERMLINECNVCALLS.out.segments // channel: [ val(meta), path(*.tar.gz) ] + genotyped_filtered_segments_vcf = BCFTOOLS_VIEW.out.vcf // channel: [ val(meta), path(*.tar.gz) ] + denoised_vcf = GATK4_POSTPROCESSGERMLINECNVCALLS.out.denoised // channel: [ val(meta), path(*.tar.gz) ] + versions = ch_versions // channel: [ versions.yml ] } // This function groups calls with same meta for postprocessing. From f6db87dd790dc626a065fb9e65d293a827c2640d Mon Sep 17 00:00:00 2001 From: Jakob Willforss Date: Fri, 22 Dec 2023 10:12:36 +0100 Subject: [PATCH 196/387] Remove ref-option again, and iinclude bcftools config --- conf/modules/call_sv_germlinecnvcaller.config | 5 +++++ modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf | 3 +-- 2 files changed, 6 insertions(+), 2 deletions(-) diff --git a/conf/modules/call_sv_germlinecnvcaller.config b/conf/modules/call_sv_germlinecnvcaller.config index 8539e546..c41251ae 100644 --- a/conf/modules/call_sv_germlinecnvcaller.config +++ b/conf/modules/call_sv_germlinecnvcaller.config @@ -37,5 +37,10 @@ process { ext.args = "--run-mode CASE" ext.prefix = { "${meta.id}_${model.simpleName}" } } + + withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_VIEW' { + ext.prefix = { "${meta.id}_gatkcnv_segments_reffiltered" } + ext.args = { '--output-type z --exclude "N_ALT = 0" ' } + } } } diff --git a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf index 98652570..d916c2af 100644 --- a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf +++ b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf @@ -41,8 +41,7 @@ process GATK4_POSTPROCESSGERMLINECNVCALLS { $ploidy_command \\ --output-genotyped-intervals ${prefix}_genotyped_intervals.vcf.gz \\ --output-genotyped-segments ${prefix}_genotyped_segments.vcf.gz \\ - --output-denoised-copy-ratios ${prefix}_denoised.vcf.gz \\ - --sites-only-vcf-output + --output-denoised-copy-ratios ${prefix}_denoised.vcf.gz cat <<-END_VERSIONS > versions.yml "${task.process}": From 32e42dbf189ace74d33513aa937736de5c0d35e0 Mon Sep 17 00:00:00 2001 From: Jakob Willforss Date: Fri, 22 Dec 2023 15:32:27 +0100 Subject: [PATCH 197/387] Grab the correct segments file outside the GATK CNV caller --- subworkflows/local/call_structural_variants.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/call_structural_variants.nf b/subworkflows/local/call_structural_variants.nf index e16275c9..ad45dc29 100644 --- a/subworkflows/local/call_structural_variants.nf +++ b/subworkflows/local/call_structural_variants.nf @@ -46,7 +46,7 @@ workflow CALL_STRUCTURAL_VARIANTS { if (!params.skip_germlinecnvcaller) { CALL_SV_GERMLINECNVCALLER (ch_genome_bam_bai, ch_genome_fasta, ch_genome_fai, ch_readcount_intervals, ch_genome_dictionary, ch_ploidy_model, ch_gcnvcaller_model) - .genotyped_segments_vcf + .genotyped_filtered_segments_vcf .collect{it[1]} .set { gcnvcaller_vcf } From c38762323363186c63959fa54c4e6181c37418c4 Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 4 Jan 2024 11:49:25 +0100 Subject: [PATCH 198/387] retroseq call subworkflow --- assets/grch37_g1k_alu.bed | 0 assets/grch37_g1k_herv.bed | 0 assets/grch37_g1k_l1.bed | 0 assets/grch37_g1k_sva.bed | 0 assets/mobile_element_references.tsv | 5 ++ assets/mobile_element_references_schema.json | 26 ++++++ conf/modules/call_mobile_elements.config | 40 +++++++++ conf/test.config | 2 + main.nf | 3 +- modules/local/retroseq/call/main.nf | 8 +- modules/local/retroseq/call/meta.yml | 6 +- modules/local/retroseq/discover/main.nf | 9 +- modules/local/retroseq/discover/meta.yml | 14 +-- nextflow.config | 2 + nextflow_schema.json | 9 ++ .../local/annotate_mobile_elements.nf | 46 ---------- subworkflows/local/call_mobile_elements.nf | 86 +++++++++++++++++++ workflows/raredisease.nf | 11 +++ 18 files changed, 206 insertions(+), 61 deletions(-) create mode 100644 assets/grch37_g1k_alu.bed create mode 100644 assets/grch37_g1k_herv.bed create mode 100644 assets/grch37_g1k_l1.bed create mode 100644 assets/grch37_g1k_sva.bed create mode 100644 assets/mobile_element_references.tsv create mode 100644 assets/mobile_element_references_schema.json create mode 100644 conf/modules/call_mobile_elements.config delete mode 100644 subworkflows/local/annotate_mobile_elements.nf create mode 100644 subworkflows/local/call_mobile_elements.nf diff --git a/assets/grch37_g1k_alu.bed b/assets/grch37_g1k_alu.bed new file mode 100644 index 00000000..e69de29b diff --git a/assets/grch37_g1k_herv.bed b/assets/grch37_g1k_herv.bed new file mode 100644 index 00000000..e69de29b diff --git a/assets/grch37_g1k_l1.bed b/assets/grch37_g1k_l1.bed new file mode 100644 index 00000000..e69de29b diff --git a/assets/grch37_g1k_sva.bed b/assets/grch37_g1k_sva.bed new file mode 100644 index 00000000..e69de29b diff --git a/assets/mobile_element_references.tsv b/assets/mobile_element_references.tsv new file mode 100644 index 00000000..328e5083 --- /dev/null +++ b/assets/mobile_element_references.tsv @@ -0,0 +1,5 @@ +type path +L1 grch37_g1k_l1.bed +SVA grch37_g1k_sva.bed +ALU grch37_g1k_alu.bed +HERV grch37_g1k_herv.bed diff --git a/assets/mobile_element_references_schema.json b/assets/mobile_element_references_schema.json new file mode 100644 index 00000000..40e79825 --- /dev/null +++ b/assets/mobile_element_references_schema.json @@ -0,0 +1,26 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/raredisease/master/assets/mobile_element_references_schema.json", + "title": "Schema for mobile_element_references", + "description": "Schema for the file provided with params.mobile_element_references", + "type": "array", + "items": { + "type": "object", + "properties": { + "type": { + "type": "string", + "exists": true, + "pattern": "^\\S+$", + "errorMessage": "Mobile element type must be provided and cannot contain spaces" + }, + "path": { + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.bed$", + "errorMessage": "Bed file, cannot contain spaces and must have extension '.bed'" + } + }, + "required": ["type", "path"] + } +} diff --git a/conf/modules/call_mobile_elements.config b/conf/modules/call_mobile_elements.config new file mode 100644 index 00000000..b7a0b81f --- /dev/null +++ b/conf/modules/call_mobile_elements.config @@ -0,0 +1,40 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. + ext.when = Conditional clause +---------------------------------------------------------------------------------------- +*/ + +process { + + withName: '.*CALL_MOBILE_ELEMENTS:.*' { + publishDir = [ + enabled: false + ] + } + + withName: '.*CALL_MOBILE_ELEMENTS:ME_SPLIT_ALIGNMENT' { + ext.args = { [ + '--output-fmt bam', + '--fetch-pairs' + ].join(' ') } + ext.args2 = { "${meta.interval}" } + ext.prefix = { "${meta.id}_${meta.interval}" } + } + + withName: '.*CALL_MOBILE_ELEMENTS:RETROSEQ_DISCOVER' { + ext.prefix = { "${meta.id}_${meta.interval}_retroseq_discover" } + } + + withName: '.*CALL_MOBILE_ELEMENTS:RETROSEQ_CALL' { + ext.args = { '--soft' } + ext.prefix = { "${meta.id}_${meta.interval}_retroseq_call" } + } + +} diff --git a/conf/test.config b/conf/test.config index d398d542..e23102f8 100644 --- a/conf/test.config +++ b/conf/test.config @@ -41,6 +41,8 @@ params { intervals_wgs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/target_wgs.interval_list" intervals_y = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/targetY.interval_list" known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" + // TODO: Update path for mobile element refs + mobile_element_references = "${projectDir}//mobile_element_references.tsv" ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" score_config_mt = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" diff --git a/main.nf b/main.nf index 9d24f1ac..5b0bb182 100644 --- a/main.nf +++ b/main.nf @@ -16,7 +16,7 @@ nextflow.enable.dsl = 2 GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ - +// TODO: Are the genome config used? params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') params.fai = WorkflowMain.getGenomeAttribute(params, 'fai') params.bwa = WorkflowMain.getGenomeAttribute(params, 'bwa') @@ -33,6 +33,7 @@ params.intervals_wgs = WorkflowMain.getGenomeAttribute(params, params.intervals_y = WorkflowMain.getGenomeAttribute(params, 'intervals_y') params.known_dbsnp = WorkflowMain.getGenomeAttribute(params, 'known_dbsnp') params.known_dbsnp_tbi = WorkflowMain.getGenomeAttribute(params, 'known_dbsnp_tbi') +params.mobile_element_references = WorkflowMain.getGenomeAttribute(params, 'mobile_element_references') params.ml_model = WorkflowMain.getGenomeAttribute(params, 'ml_model') params.mt_fasta = WorkflowMain.getGenomeAttribute(params, 'mt_fasta') params.ngsbits_samplegender_method = WorkflowMain.getGenomeAttribute(params, 'ngsbits_samplegender_method') diff --git a/modules/local/retroseq/call/main.nf b/modules/local/retroseq/call/main.nf index 569d84a2..21207f9b 100644 --- a/modules/local/retroseq/call/main.nf +++ b/modules/local/retroseq/call/main.nf @@ -9,6 +9,7 @@ process RETROSEQ_CALL { input: tuple val(meta), path(bam), path(bai) + tuple val(meta), path(input) tuple val(meta), path(fasta) output: @@ -26,10 +27,11 @@ process RETROSEQ_CALL { """ retroseq.pl \\ -call \\ + $args \\ -bam $bam \\ - -input $input + -input $input \\ -ref $fasta \\ - -output ${prefix}.tab + -output ${prefix}.vcf cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -42,7 +44,7 @@ process RETROSEQ_CALL { def prefix = task.ext.prefix ?: "${meta.id}" def VERSION = "1.5" """ - touch ${prefix}.tab + touch ${prefix}.vcf cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/local/retroseq/call/meta.yml b/modules/local/retroseq/call/meta.yml index 7427491f..8147d20c 100644 --- a/modules/local/retroseq/call/meta.yml +++ b/modules/local/retroseq/call/meta.yml @@ -29,8 +29,12 @@ input: pattern: "*.{bam}" - fasta: type: file - description: reference genome in fasta format + description: Reference genome in fasta format pattern: "*.{fasta}" + - tab: + type: file + description: Output file from running retroseq -call + pattern: "*.{tab}" output: - meta: diff --git a/modules/local/retroseq/discover/main.nf b/modules/local/retroseq/discover/main.nf index d71fd3fa..b72f10c3 100644 --- a/modules/local/retroseq/discover/main.nf +++ b/modules/local/retroseq/discover/main.nf @@ -9,8 +9,8 @@ process RETROSEQ_DISCOVER { input: tuple val(meta), path(bam), path(bai) - path(tab) - path(extra_files) + path(me_references) + val(me_types) output: tuple val(meta), path("*.tab"), emit: tab @@ -25,10 +25,12 @@ process RETROSEQ_DISCOVER { def VERSION = "1.5" // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. """ + paste <(printf "%s\\n" $me_types | tr -d '[],') <(printf "%s\\n" $me_references) > me_reference_manifest.tsv retroseq.pl \\ -discover \\ + $args \\ -bam $bam \\ - -refTEs $tab\\ + -refTEs me_reference_manifest.tsv\\ -output ${prefix}.tab cat <<-END_VERSIONS > versions.yml @@ -42,6 +44,7 @@ process RETROSEQ_DISCOVER { def prefix = task.ext.prefix ?: "${meta.id}" def VERSION = "1.5" """ + paste <(printf "%s\\n" $me_types | tr -d '[],') <(printf "%s\\n" $me_references) > me_reference_manifest.tsv touch ${prefix}.tab cat <<-END_VERSIONS > versions.yml diff --git a/modules/local/retroseq/discover/meta.yml b/modules/local/retroseq/discover/meta.yml index 80c6af6c..55d6de5d 100644 --- a/modules/local/retroseq/discover/meta.yml +++ b/modules/local/retroseq/discover/meta.yml @@ -26,14 +26,14 @@ input: - bai: type: file description: Index of the sorted BAM file - pattern: "*.{bam}" - - tab: - type: file - description: Tab file containing a list of files, where each line is a TE type and the path to a bed file containing its coordinates in the genome. - pattern: "*.{tab}" - - extra_files: + pattern: "*.{bai}" + - me_references: type: file - description: List of files. Each file contains the coordinates of transposable elements of a particular type. + description: Paths to bed files containing transposable element coordinates in the genome. + pattern: "*.{bed}" + - me_types: + type: list + description: List of transposable element types to discover. Needs to be in sync with me_references. output: - meta: diff --git a/nextflow.config b/nextflow.config index e65b343d..4c4a4be8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -45,6 +45,7 @@ params { // File params svdb_query_bedpedbs = null svdb_query_dbs = null + mobile_element_references = null // Alignment aligner = 'bwamem2' @@ -329,6 +330,7 @@ includeConfig 'conf/modules/call_sv_germlinecnvcaller.config' includeConfig 'conf/modules/call_sv_manta.config' includeConfig 'conf/modules/call_sv_tiddit.config' includeConfig 'conf/modules/postprocess_MT_calls.config' +includeConfig 'conf/modules/call_mobile_elements.config' // Function to ensure that resource requirements don't go beyond // a maximum limit diff --git a/nextflow_schema.json b/nextflow_schema.json index 07988ec3..76f6a336 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -214,6 +214,15 @@ "help_text": "Used to extract relevant information from the references to analyse mitochondria", "fa_icon": "fas fa-align-center" }, + "mobile_element_references": { + "type": "string", + "fa_icon": "fas fa-file", + "description": "File with mobile element references", + "help_text": "Path to tsv file listing mobile element references. \nFormat: \\t", + "pattern": "^\\S+\\.tsv$", + "format": "file-path", + "schema": "assets/mobile_element_references_schema.json" + }, "ml_model": { "type": "string", "exists": true, diff --git a/subworkflows/local/annotate_mobile_elements.nf b/subworkflows/local/annotate_mobile_elements.nf deleted file mode 100644 index 26648434..00000000 --- a/subworkflows/local/annotate_mobile_elements.nf +++ /dev/null @@ -1,46 +0,0 @@ -// -// A subworkflow to annotate mobile elements in the genome -// - -include { RETROSEQ_CALL as RETROSEQ_CALL } from '../../modules/local/retroseq/call/main' -include { RETROSEQ_DISCOVER as RETROSEQ_DISCOVER } from '../../modules/local/retroseq/discover/main' - -workflow ANNOTATE_STRUCTURAL_VARIANTS { - - take: - ch_bam // channel: [mandatory] [ val(meta), path(bam) ] - ch_bai // channel: [mandatory] [ val(meta), path(bai) ] - ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] - ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] - - main: - ch_versions = Channel.empty() - - // Running retroseq discover: identify discordant read pairs that might support a TE insertion - RETROSEQ_DISCOVER ( - ch_bam_bai, - "tab", - "extrafiles" - ) - - // Running retroseq call: clusters reads and checks on the breakpoints to decide whether a TEV is present - RETROSEQ_CALL ( - ch_bam_bai, - ch_genome_fasta, - ) - - // Run vep to annotate - - // Run svdb to query against database - - // Filter and rank as done in findtroll? E.g. protein coding - - ch_versions = ch_versions.mix(RETROSEQ_CALL.out.versions) - ch_versions = ch_versions.mix(RETROSEQ_DISCOVER.out.versions) - - emit: - vcf_ann = RETROSEQ_CALL.out.vcf // channel: [ val(meta), path(vcf) ] - versions = ch_versions // channel: [ path(versions.yml) ] -} - diff --git a/subworkflows/local/call_mobile_elements.nf b/subworkflows/local/call_mobile_elements.nf new file mode 100644 index 00000000..32b62bac --- /dev/null +++ b/subworkflows/local/call_mobile_elements.nf @@ -0,0 +1,86 @@ +// +// A subworkflow to call mobile elements in the genome +// + +include { RETROSEQ_CALL as RETROSEQ_CALL } from '../../modules/local/retroseq/call/main' +include { RETROSEQ_DISCOVER as RETROSEQ_DISCOVER } from '../../modules/local/retroseq/discover/main' +include { SAMTOOLS_VIEW as ME_SPLIT_ALIGNMENT } from '../../modules/nf-core/samtools/view/main' +include { SAMTOOLS_INDEX as ME_INDEX_SPLIT_ALIGNMENT } from '../../modules/nf-core/samtools/index/main' + +workflow CALL_MOBILE_ELEMENTS { + + take: + ch_genome_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_me_references // channel: [mandatory] [path(tsv)] + val_genome_build // string: [mandatory] GRCh37 or GRCh38 + + main: + ch_versions = Channel.empty() + + // Building chromosome channel depending on genome version + // TODO: Check how retroseq behaves when running chrY on female samples + Channel.of(1..22, 'X', 'Y') + .branch { it -> + grch37: val_genome_build.equals('GRCh37') + return [it.toString()] + grch38: val_genome_build.equals('GRCh38') + return ['chr' + it.toString()] + }.set{ ch_chr_genome } + ch_chr = ch_chr_genome.grch37.mix(ch_chr_genome.grch38) + + // Building one bam channel per chromosome and adding interval + ch_genome_bam_bai + .combine(ch_chr) + .map { + meta, bam, bai, chr -> + return [ meta + [interval:chr], bam, bai ] + } + .set { ch_genome_bam_bai_interval } + + ME_SPLIT_ALIGNMENT( + ch_genome_bam_bai_interval, + [[:], []], + [] + ) + + ME_INDEX_SPLIT_ALIGNMENT( ME_SPLIT_ALIGNMENT.out.bam ) + + ME_SPLIT_ALIGNMENT.out.bam + .join(ME_INDEX_SPLIT_ALIGNMENT.out.bai, failOnMismatch:true) + .set { ch_retroseq_input } + + ch_me_references + .multiMap { type, path -> + type: type + path: path + } + .set { ch_me_reference_split } + + RETROSEQ_DISCOVER ( + ch_retroseq_input, + ch_me_reference_split.path.collect(), + ch_me_reference_split.type.collect() + ) + + // Running retroseq call: clusters reads and checks on the breakpoints to decide whether a TEV is present + RETROSEQ_CALL ( + ch_retroseq_input, + RETROSEQ_DISCOVER.out.tab, + ch_genome_fasta + ) + + // Run vep to annotate + + // Run svdb to query against database + + // Filter and rank as done in findtroll? E.g. protein coding + + ch_versions = ch_versions.mix(ME_SPLIT_ALIGNMENT.out.versions) + ch_versions = ch_versions.mix(ME_INDEX_SPLIT_ALIGNMENT.out.versions) + ch_versions = ch_versions.mix(RETROSEQ_DISCOVER.out.versions) + ch_versions = ch_versions.mix(RETROSEQ_CALL.out.versions) + + emit: + versions = ch_versions // channel: [ path(versions.yml) ] +} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index c4183c93..f572a651 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -133,6 +133,7 @@ include { RANK_VARIANTS as RANK_VARIANTS_MT } from '../subworkflows/local/ra include { RANK_VARIANTS as RANK_VARIANTS_SNV } from '../subworkflows/local/rank_variants' include { RANK_VARIANTS as RANK_VARIANTS_SV } from '../subworkflows/local/rank_variants' include { SCATTER_GENOME } from '../subworkflows/local/scatter_genome' +include { CALL_MOBILE_ELEMENTS } from '../subworkflows/local/call_mobile_elements' /* @@ -241,6 +242,9 @@ workflow RAREDISEASE { : Channel.empty() ch_intervals_y = params.intervals_y ? Channel.fromPath(params.intervals_y).collect() : Channel.empty() + //ch_me_references = params.mobile_element_references ? Channel.fromPath(params.mobile_element_references) + ch_me_references = params.mobile_element_references ? Channel.fromSamplesheet("mobile_element_references") + : Channel.empty() ch_ml_model = params.variant_caller.equals("sentieon") ? Channel.fromPath(params.ml_model).map {it -> [[id:it[0].simpleName], it]}.collect() : Channel.value([[:],[]]) ch_mt_intervals = ch_references.mt_intervals @@ -595,6 +599,13 @@ workflow RAREDISEASE { ch_versions = ch_versions.mix(GENS.out.versions) } + CALL_MOBILE_ELEMENTS( + ch_mapped.genome_bam_bai, + ch_genome_fasta, + ch_me_references, + params.genome + ) + // // MODULE: Pipeline reporting // From cad46c183c24594f7e2e2e50c739660022335a8f Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 4 Jan 2024 11:57:46 +0100 Subject: [PATCH 199/387] updating temporary ref_me paths --- assets/mobile_element_references.tsv | 8 ++++---- conf/test.config | 2 +- 2 files changed, 5 insertions(+), 5 deletions(-) diff --git a/assets/mobile_element_references.tsv b/assets/mobile_element_references.tsv index 328e5083..e5a804ff 100644 --- a/assets/mobile_element_references.tsv +++ b/assets/mobile_element_references.tsv @@ -1,5 +1,5 @@ type path -L1 grch37_g1k_l1.bed -SVA grch37_g1k_sva.bed -ALU grch37_g1k_alu.bed -HERV grch37_g1k_herv.bed +L1 ./assets/grch37_g1k_l1.bed +SVA ./assets/grch37_g1k_sva.bed +ALU ./assets/grch37_g1k_alu.bed +HERV ./assets/grch37_g1k_herv.bed diff --git a/conf/test.config b/conf/test.config index e23102f8..e691a70d 100644 --- a/conf/test.config +++ b/conf/test.config @@ -42,7 +42,7 @@ params { intervals_y = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/targetY.interval_list" known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" // TODO: Update path for mobile element refs - mobile_element_references = "${projectDir}//mobile_element_references.tsv" + mobile_element_references = "${projectDir}/assets/mobile_element_references.tsv" ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" score_config_mt = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" From 0f6edbdfccd6ae89140c29f7ecd666e356f074ea Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 4 Jan 2024 12:57:05 +0100 Subject: [PATCH 200/387] fixing path to retroseq docker --- modules/local/retroseq/call/main.nf | 2 +- modules/local/retroseq/discover/main.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/retroseq/call/main.nf b/modules/local/retroseq/call/main.nf index 21207f9b..33ddb1af 100644 --- a/modules/local/retroseq/call/main.nf +++ b/modules/local/retroseq/call/main.nf @@ -4,7 +4,7 @@ process RETROSEQ_CALL { conda "bioconda::perl-retroseq=1.5=pl5321hdfd78af_1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'docker://clinicalgenomics/retroseq:1.5_9d4f3b5' : 'docker://clinicalgenomics/retroseq:1.5_9d4f3b5' }" + 'docker.io/clinicalgenomics/retroseq:1.5_9d4f3b5' : 'docker.io/clinicalgenomics/retroseq:1.5_9d4f3b5' }" input: diff --git a/modules/local/retroseq/discover/main.nf b/modules/local/retroseq/discover/main.nf index b72f10c3..cf4b5fa8 100644 --- a/modules/local/retroseq/discover/main.nf +++ b/modules/local/retroseq/discover/main.nf @@ -4,7 +4,7 @@ process RETROSEQ_DISCOVER { conda "bioconda::perl-retroseq=1.5=pl5321hdfd78af_1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'docker://clinicalgenomics/retroseq:1.5_9d4f3b5' : 'docker://clinicalgenomics/retroseq:1.5_9d4f3b5' }" + 'docker.io/clinicalgenomics/retroseq:1.5_9d4f3b5' : 'docker.io/clinicalgenomics/retroseq:1.5_9d4f3b5' }" input: From bddd2fc7376cb972900855141c91190cbd3c8d11 Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 4 Jan 2024 13:02:49 +0100 Subject: [PATCH 201/387] propagating versions --- subworkflows/local/call_mobile_elements.nf | 10 +++++----- workflows/raredisease.nf | 1 + 2 files changed, 6 insertions(+), 5 deletions(-) diff --git a/subworkflows/local/call_mobile_elements.nf b/subworkflows/local/call_mobile_elements.nf index 32b62bac..9929fb7f 100644 --- a/subworkflows/local/call_mobile_elements.nf +++ b/subworkflows/local/call_mobile_elements.nf @@ -45,7 +45,7 @@ workflow CALL_MOBILE_ELEMENTS { ) ME_INDEX_SPLIT_ALIGNMENT( ME_SPLIT_ALIGNMENT.out.bam ) - + ME_SPLIT_ALIGNMENT.out.bam .join(ME_INDEX_SPLIT_ALIGNMENT.out.bai, failOnMismatch:true) .set { ch_retroseq_input } @@ -76,10 +76,10 @@ workflow CALL_MOBILE_ELEMENTS { // Filter and rank as done in findtroll? E.g. protein coding - ch_versions = ch_versions.mix(ME_SPLIT_ALIGNMENT.out.versions) - ch_versions = ch_versions.mix(ME_INDEX_SPLIT_ALIGNMENT.out.versions) - ch_versions = ch_versions.mix(RETROSEQ_DISCOVER.out.versions) - ch_versions = ch_versions.mix(RETROSEQ_CALL.out.versions) + ch_versions = ch_versions.mix(ME_SPLIT_ALIGNMENT.out.versions).first() + ch_versions = ch_versions.mix(ME_INDEX_SPLIT_ALIGNMENT.out.versions).first() + ch_versions = ch_versions.mix(RETROSEQ_DISCOVER.out.versions).first() + ch_versions = ch_versions.mix(RETROSEQ_CALL.out.versions).first() emit: versions = ch_versions // channel: [ path(versions.yml) ] diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index f572a651..6d833d08 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -605,6 +605,7 @@ workflow RAREDISEASE { ch_me_references, params.genome ) + ch_versions = ch_versions.mix(CALL_MOBILE_ELEMENTS.out.versions) // // MODULE: Pipeline reporting From 0c0fd91447508533530a6df090d050a7dad2c676 Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 4 Jan 2024 15:58:56 +0100 Subject: [PATCH 202/387] joining channels to keep them in sync --- modules/local/retroseq/call/main.nf | 8 ++++---- modules/local/retroseq/call/meta.yml | 18 +++++++++++------- modules/local/retroseq/discover/main.nf | 2 +- modules/local/retroseq/discover/meta.yml | 8 ++++---- subworkflows/local/call_mobile_elements.nf | 12 +++++++++--- subworkflows/local/call_structural_variants.nf | 11 ++++++----- workflows/raredisease.nf | 5 ++++- 7 files changed, 39 insertions(+), 25 deletions(-) diff --git a/modules/local/retroseq/call/main.nf b/modules/local/retroseq/call/main.nf index 33ddb1af..150af4ba 100644 --- a/modules/local/retroseq/call/main.nf +++ b/modules/local/retroseq/call/main.nf @@ -4,13 +4,13 @@ process RETROSEQ_CALL { conda "bioconda::perl-retroseq=1.5=pl5321hdfd78af_1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'docker.io/clinicalgenomics/retroseq:1.5_9d4f3b5' : 'docker.io/clinicalgenomics/retroseq:1.5_9d4f3b5' }" + 'docker.io/clinicalgenomics/retroseq:1.5_9d4f3b5-1' : 'docker.io/clinicalgenomics/retroseq:1.5_9d4f3b5-1' }" input: - tuple val(meta), path(bam), path(bai) - tuple val(meta), path(input) - tuple val(meta), path(fasta) + tuple val(meta), path(tab), path(bam), path(bai) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fai) output: tuple val(meta), path("*.vcf"), emit: vcf diff --git a/modules/local/retroseq/call/meta.yml b/modules/local/retroseq/call/meta.yml index 8147d20c..98aa0c04 100644 --- a/modules/local/retroseq/call/meta.yml +++ b/modules/local/retroseq/call/meta.yml @@ -19,22 +19,26 @@ input: description: | Groovy Map containing sample information e.g. `[ id:'test', single_end:false ]` + - tab: + type: file + description: Output file from running retroseq -call + pattern: "*.tab" - bam: type: file description: Sorted BAM file - pattern: "*.{bam}" + pattern: "*.bam" - bai: type: file description: Index of the sorted BAM file - pattern: "*.{bam}" + pattern: "*.bam" - fasta: type: file description: Reference genome in fasta format - pattern: "*.{fasta}" - - tab: + pattern: "*.fasta" + - fai: type: file - description: Output file from running retroseq -call - pattern: "*.{tab}" + description: Reference FASTA index + pattern: "*.fai" output: - meta: @@ -49,7 +53,7 @@ output: - vcf: type: file description: Output file containing TEVs and their location in the genome. - pattern: "*.{vcf}" + pattern: "*.vcf" authors: - "@peterpru" diff --git a/modules/local/retroseq/discover/main.nf b/modules/local/retroseq/discover/main.nf index cf4b5fa8..2ea51344 100644 --- a/modules/local/retroseq/discover/main.nf +++ b/modules/local/retroseq/discover/main.nf @@ -4,7 +4,7 @@ process RETROSEQ_DISCOVER { conda "bioconda::perl-retroseq=1.5=pl5321hdfd78af_1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'docker.io/clinicalgenomics/retroseq:1.5_9d4f3b5' : 'docker.io/clinicalgenomics/retroseq:1.5_9d4f3b5' }" + 'docker.io/clinicalgenomics/retroseq:1.5_9d4f3b5-1' : 'docker.io/clinicalgenomics/retroseq:1.5_9d4f3b5-1' }" input: diff --git a/modules/local/retroseq/discover/meta.yml b/modules/local/retroseq/discover/meta.yml index 55d6de5d..9ea47408 100644 --- a/modules/local/retroseq/discover/meta.yml +++ b/modules/local/retroseq/discover/meta.yml @@ -22,15 +22,15 @@ input: - bam: type: file description: Sorted BAM file - pattern: "*.{bam}" + pattern: "*.bam" - bai: type: file description: Index of the sorted BAM file - pattern: "*.{bai}" + pattern: "*.bai" - me_references: type: file description: Paths to bed files containing transposable element coordinates in the genome. - pattern: "*.{bed}" + pattern: "*.bed" - me_types: type: list description: List of transposable element types to discover. Needs to be in sync with me_references. @@ -48,7 +48,7 @@ output: - tab: type: file description: Output file containing lists of read pair names per TE type - pattern: "*.{tab}" + pattern: "*.tab" authors: - "@peterpru" diff --git a/subworkflows/local/call_mobile_elements.nf b/subworkflows/local/call_mobile_elements.nf index 9929fb7f..e2d34752 100644 --- a/subworkflows/local/call_mobile_elements.nf +++ b/subworkflows/local/call_mobile_elements.nf @@ -12,6 +12,7 @@ workflow CALL_MOBILE_ELEMENTS { take: ch_genome_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] ch_me_references // channel: [mandatory] [path(tsv)] val_genome_build // string: [mandatory] GRCh37 or GRCh38 @@ -63,13 +64,18 @@ workflow CALL_MOBILE_ELEMENTS { ch_me_reference_split.type.collect() ) + RETROSEQ_DISCOVER.out.tab + .join(ch_retroseq_input, failOnMismatch: true) + .set { ch_retroseq_call_input } + // Running retroseq call: clusters reads and checks on the breakpoints to decide whether a TEV is present RETROSEQ_CALL ( - ch_retroseq_input, - RETROSEQ_DISCOVER.out.tab, - ch_genome_fasta + ch_retroseq_call_input, + ch_genome_fasta, + ch_genome_fai ) + // Run vep to annotate // Run svdb to query against database diff --git a/subworkflows/local/call_structural_variants.nf b/subworkflows/local/call_structural_variants.nf index 13827f61..ee5c2cbc 100644 --- a/subworkflows/local/call_structural_variants.nf +++ b/subworkflows/local/call_structural_variants.nf @@ -53,10 +53,11 @@ workflow CALL_STRUCTURAL_VARIANTS { ch_versions = ch_versions.mix(CALL_SV_GERMLINECNVCALLER.out.versions) } - CALL_SV_CNVNATOR (ch_genome_bam_bai, ch_genome_fasta, ch_genome_fai, ch_case_info) - .vcf - .collect{it[1]} - .set { cnvnator_vcf } + // Uncomment + //CALL_SV_CNVNATOR (ch_genome_bam_bai, ch_genome_fasta, ch_genome_fai, ch_case_info) + // .vcf + // .collect{it[1]} + // .set { cnvnator_vcf } CALL_SV_MT (ch_mt_bam_bai, ch_genome_fasta) @@ -64,7 +65,7 @@ workflow CALL_STRUCTURAL_VARIANTS { if (params.skip_germlinecnvcaller) { tiddit_vcf .combine(manta_vcf) - .combine(cnvnator_vcf) + // .combine(cnvnator_vcf) .toList() .set { vcf_list } } else { diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 6d833d08..f416ec4d 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -293,7 +293,9 @@ workflow RAREDISEASE { // SV caller priority if (params.skip_germlinecnvcaller) { - ch_svcaller_priority = Channel.value(["tiddit", "manta", "cnvnator"]) + // TODO: Uncomment and remove + ch_svcaller_priority = Channel.value(["tiddit", "manta"]) + //ch_svcaller_priority = Channel.value(["tiddit", "manta", "cnvnator"]) } else { ch_svcaller_priority = Channel.value(["tiddit", "manta", "gcnvcaller", "cnvnator"]) } @@ -602,6 +604,7 @@ workflow RAREDISEASE { CALL_MOBILE_ELEMENTS( ch_mapped.genome_bam_bai, ch_genome_fasta, + ch_genome_fai, ch_me_references, params.genome ) From ad9051841d39aafd4c93be0dd93c2d0a5cb24e53 Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 4 Jan 2024 17:52:23 +0100 Subject: [PATCH 203/387] fix input name --- modules/local/retroseq/call/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/retroseq/call/main.nf b/modules/local/retroseq/call/main.nf index 150af4ba..5b13c630 100644 --- a/modules/local/retroseq/call/main.nf +++ b/modules/local/retroseq/call/main.nf @@ -29,7 +29,7 @@ process RETROSEQ_CALL { -call \\ $args \\ -bam $bam \\ - -input $input \\ + -input $tab \\ -ref $fasta \\ -output ${prefix}.vcf From caa0029cc35357f3096c6218fd0c00dd6dbf231c Mon Sep 17 00:00:00 2001 From: jemten Date: Fri, 5 Jan 2024 14:37:28 +0100 Subject: [PATCH 204/387] concatenate and merge vcfs --- conf/modules/call_mobile_elements.config | 33 ++++++++++ subworkflows/local/call_mobile_elements.nf | 73 +++++++++++++++++----- workflows/raredisease.nf | 1 + 3 files changed, 93 insertions(+), 14 deletions(-) diff --git a/conf/modules/call_mobile_elements.config b/conf/modules/call_mobile_elements.config index b7a0b81f..788404a3 100644 --- a/conf/modules/call_mobile_elements.config +++ b/conf/modules/call_mobile_elements.config @@ -37,4 +37,37 @@ process { ext.prefix = { "${meta.id}_${meta.interval}_retroseq_call" } } + withName: '.*CALL_MOBILE_ELEMENTS:BCFTOOLS_REHEADER_ME' { + ext.args2 = { '--output-type v' } + ext.prefix = { "${meta.id}_${meta.interval}_retroseq_reheader" } + } + + withName: '.*CALL_MOBILE_ELEMENTS:BCFTOOLS_SORT_ME' { + ext.args = { '--output-type z' } + ext.prefix = { "${meta.id}_${meta.interval}_retroseq_sort" } + } + + withName: '.*CALL_MOBILE_ELEMENTS:BCFTOOLS_CONCAT_ME' { + ext.args = { '--output-type z --allow-overlaps' } + ext.prefix = { "${meta.id}_mobile_elements" } + } + + withName: '.*CALL_MOBILE_ELEMENTS:SVDB_MERGE_ME' { + ext.args = { '--bnd_distance 150 --overlap 0.5' } + ext.prefix = { "${meta.id}_mobile_elements" } + publishDir = [ + path: { "${params.outdir}/call_mobile_elements" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*CALL_MOBILE_ELEMENTS:TABIX_ME' { + publishDir = [ + path: { "${params.outdir}/call_mobile_elements" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + } diff --git a/subworkflows/local/call_mobile_elements.nf b/subworkflows/local/call_mobile_elements.nf index e2d34752..7db198be 100644 --- a/subworkflows/local/call_mobile_elements.nf +++ b/subworkflows/local/call_mobile_elements.nf @@ -2,10 +2,16 @@ // A subworkflow to call mobile elements in the genome // +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_ME } from '../../modules/nf-core/bcftools/reheader/main' +include { BCFTOOLS_CONCAT as BCFTOOLS_CONCAT_ME } from '../../modules/nf-core/bcftools/concat/main' +include { BCFTOOLS_SORT as BCFTOOLS_SORT_ME } from '../../modules/nf-core/bcftools/sort/main' include { RETROSEQ_CALL as RETROSEQ_CALL } from '../../modules/local/retroseq/call/main' include { RETROSEQ_DISCOVER as RETROSEQ_DISCOVER } from '../../modules/local/retroseq/discover/main' -include { SAMTOOLS_VIEW as ME_SPLIT_ALIGNMENT } from '../../modules/nf-core/samtools/view/main' include { SAMTOOLS_INDEX as ME_INDEX_SPLIT_ALIGNMENT } from '../../modules/nf-core/samtools/index/main' +include { SAMTOOLS_VIEW as ME_SPLIT_ALIGNMENT } from '../../modules/nf-core/samtools/view/main' +include { TABIX_TABIX as TABIX_ME } from '../../modules/nf-core/tabix/tabix/main' +include { TABIX_TABIX as TABIX_ME_SPLIT } from '../../modules/nf-core/tabix/tabix/main' +include { SVDB_MERGE as SVDB_MERGE_ME } from '../../modules/nf-core/svdb/merge/main' workflow CALL_MOBILE_ELEMENTS { @@ -14,6 +20,7 @@ workflow CALL_MOBILE_ELEMENTS { ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] ch_me_references // channel: [mandatory] [path(tsv)] + ch_case_info // channel: [mandatory] [ val(case_info) ] val_genome_build // string: [mandatory] GRCh37 or GRCh38 main: @@ -28,7 +35,8 @@ workflow CALL_MOBILE_ELEMENTS { grch38: val_genome_build.equals('GRCh38') return ['chr' + it.toString()] }.set{ ch_chr_genome } - ch_chr = ch_chr_genome.grch37.mix(ch_chr_genome.grch38) + ch_chr_genome.grch37.mix(ch_chr_genome.grch38) + .set { ch_chr } // Building one bam channel per chromosome and adding interval ch_genome_bam_bai @@ -39,13 +47,9 @@ workflow CALL_MOBILE_ELEMENTS { } .set { ch_genome_bam_bai_interval } - ME_SPLIT_ALIGNMENT( - ch_genome_bam_bai_interval, - [[:], []], - [] - ) - - ME_INDEX_SPLIT_ALIGNMENT( ME_SPLIT_ALIGNMENT.out.bam ) + // Split bam file on chromosome and index + ME_SPLIT_ALIGNMENT ( ch_genome_bam_bai_interval, [[:], []], [] ) + ME_INDEX_SPLIT_ALIGNMENT ( ME_SPLIT_ALIGNMENT.out.bam ) ME_SPLIT_ALIGNMENT.out.bam .join(ME_INDEX_SPLIT_ALIGNMENT.out.bai, failOnMismatch:true) @@ -68,25 +72,66 @@ workflow CALL_MOBILE_ELEMENTS { .join(ch_retroseq_input, failOnMismatch: true) .set { ch_retroseq_call_input } - // Running retroseq call: clusters reads and checks on the breakpoints to decide whether a TEV is present RETROSEQ_CALL ( ch_retroseq_call_input, ch_genome_fasta, ch_genome_fai ) + // Fix the vcf by adding header, sorting and indexing + BCFTOOLS_REHEADER_ME ( + RETROSEQ_CALL.out.vcf.map{ meta, vcf -> [ meta, vcf, [] ] }, + ch_genome_fai + ) + BCFTOOLS_SORT_ME ( BCFTOOLS_REHEADER_ME.out.vcf ) + TABIX_ME_SPLIT ( BCFTOOLS_SORT_ME.out.vcf ) + + // Concatenate the chromosme vcfs per sample + BCFTOOLS_SORT_ME.out.vcf + .map { meta, vcf -> [ meta.findAll { !(it.key in ['interval']) }, vcf ] } + .groupTuple(size: 24) + .set { ch_vcfs } + + TABIX_ME_SPLIT.out.tbi + .map { meta, tbi -> [ meta.findAll { !(it.key in ['interval']) }, tbi ] } + .groupTuple(size: 24) + .set { ch_tbis } + + ch_vcfs.join(ch_tbis) + .set { ch_vcfs_tbis} + + BCFTOOLS_CONCAT_ME ( ch_vcfs_tbis ) + + // Merge sample vcfs to a case vcf + BCFTOOLS_CONCAT_ME.out.vcf + .collect{it[1]} + .toList() + .collect() + .set { ch_vcf_list } - // Run vep to annotate + ch_case_info + .combine(ch_vcf_list) + .set { ch_svdb_merge_me_input } - // Run svdb to query against database + SVDB_MERGE_ME ( ch_svdb_merge_me_input, [] ) + TABIX_ME ( SVDB_MERGE_ME.out.vcf ) - // Filter and rank as done in findtroll? E.g. protein coding + SVDB_MERGE_ME.out.vcf + .join(TABIX_ME.out.tbi) + .set { ch_me_vcf } ch_versions = ch_versions.mix(ME_SPLIT_ALIGNMENT.out.versions).first() ch_versions = ch_versions.mix(ME_INDEX_SPLIT_ALIGNMENT.out.versions).first() ch_versions = ch_versions.mix(RETROSEQ_DISCOVER.out.versions).first() ch_versions = ch_versions.mix(RETROSEQ_CALL.out.versions).first() + ch_versions = ch_versions.mix(BCFTOOLS_REHEADER_ME.out.versions).first() + ch_versions = ch_versions.mix(BCFTOOLS_SORT_ME.out.versions).first() + ch_versions = ch_versions.mix(TABIX_ME_SPLIT.out.versions).first() + ch_versions = ch_versions.mix(BCFTOOLS_CONCAT_ME.out.versions).first() + ch_versions = ch_versions.mix(SVDB_MERGE_ME.out.versions) + ch_versions = ch_versions.mix(TABIX_ME.out.versions) emit: - versions = ch_versions // channel: [ path(versions.yml) ] + me_vcf = ch_me_vcf // channel: [ val(meta), path(vcf), path(tbi) ] + versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index f416ec4d..0016a154 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -606,6 +606,7 @@ workflow RAREDISEASE { ch_genome_fasta, ch_genome_fai, ch_me_references, + ch_case_info, params.genome ) ch_versions = ch_versions.mix(CALL_MOBILE_ELEMENTS.out.versions) From c6c089039f28fc1ed9b108a55f8d2ba6cadb5d39 Mon Sep 17 00:00:00 2001 From: jemten Date: Mon, 8 Jan 2024 15:45:16 +0100 Subject: [PATCH 205/387] looking into joining error --- lib/CustomChannelOperators.groovy | 156 +++++++++++++++++++++ subworkflows/local/call_mobile_elements.nf | 19 ++- 2 files changed, 172 insertions(+), 3 deletions(-) create mode 100644 lib/CustomChannelOperators.groovy diff --git a/lib/CustomChannelOperators.groovy b/lib/CustomChannelOperators.groovy new file mode 100644 index 00000000..f1317a46 --- /dev/null +++ b/lib/CustomChannelOperators.groovy @@ -0,0 +1,156 @@ +// +// https://github.com/Midnighter/nextflow-utility-services/blob/1f84278c965255fb2bbe290b8d6c9f33f71da26f/lib/CustomChannelOperators.groovy +// +/** + * MIT License + * + * Copyright (c) 2022 Moritz E. Beber + * + * Permission is hereby granted, free of charge, to any person obtaining a copy + * of this software and associated documentation files (the "Software"), to deal + * in the Software without restriction, including without limitation the rights + * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the Software is + * furnished to do so, subject to the following conditions: + * + * The above copyright notice and this permission notice shall be included in all + * copies or substantial portions of the Software. + * + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR + * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, + * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE + * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER + * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, + * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE + * SOFTWARE. + */ + +import groovyx.gpars.dataflow.DataflowBroadcast + +/** + * Provide a collection of custom channel operators that go beyond the nextflow default. + * + * @author Moritz E. Beber + * @author Mahesh Binzer-Panchal + */ +class CustomChannelOperators { + + /** + * Join two channels by one or more keys from a map contained in each channel. + * + * The channel elements are assumed to be tuples whose size is at least two. + * Typically, the maps to join by are in the first position of the tuples. + * Please read https://www.nextflow.io/docs/latest/operator.html#join carefully. + * + * @param left The left-hand side channel in the join. + * @param right The right-hand side channel in the join. + * @param keys A list of strings providing the map keys to compare. + * @param leftBy The position of the map in the left channel. + * @param rightBy The position of the map in the right channel. + * @param joinArgs A map of keyword arguments that is passed on to the nextflow join call. + * @return The joined channels with the map in the original position of the left channel, + * followed by all elements of the right channel except for the map. + */ + static DataflowBroadcast joinOnKeys( + DataflowBroadcast left, + DataflowBroadcast right, + List keys, + Integer leftBy, + Integer rightBy, + Map joinArgs + ) { + // Extract desired keys from the left map, located at `leftBy`, and prepend them. + DataflowBroadcast newLeft = left.map { tuple -> + extractKeys(tuple, keys, leftBy) + tuple + } + + // Extract desired keys from the right map, located at `rightBy`, and prepend them. + // Also drop the map itself from the right. + DataflowBroadcast newRight = right.map { tuple -> + extractKeys(tuple, keys, rightBy) + removeMap(tuple, rightBy) + } + + // Set the positions to join on explicitly. + joinArgs.by = 0.. dropKeys(tuple, keys) } + } + + static DataflowBroadcast joinOnKeys( + Map joinArgs, + DataflowBroadcast left, + DataflowBroadcast right, + String key, + Integer leftBy = 0, + Integer rightBy = 0 + ) { + return joinOnKeys(left, right, [key], leftBy, rightBy, joinArgs) + } + + static DataflowBroadcast joinOnKeys( + DataflowBroadcast left, + DataflowBroadcast right, + String key, + Integer leftBy = 0, + Integer rightBy = 0 + ) { + return joinOnKeys(left, right, [key], leftBy, rightBy, [:]) + } + + static DataflowBroadcast joinOnKeys( + Map joinArgs, + DataflowBroadcast left, + DataflowBroadcast right, + List keys, + Integer leftBy = 0, + Integer rightBy = 0 + ) { + return joinOnKeys(left, right, keys, leftBy, rightBy, joinArgs) + } + + static DataflowBroadcast joinOnKeys( + DataflowBroadcast left, + DataflowBroadcast right, + List keys, + Integer leftBy = 0, + Integer rightBy = 0 + ) { + return joinOnKeys(left, right, keys, leftBy, rightBy, [:]) + } + + /** + * Extract values from a map at given position with given keys. + * + * @param tuple A tuple (`List`) channel element. + * @param keys A list of strings denoting keys in the map. + * @param index The position of the map in the tuple. + * @return The list of values extracted from the map. + */ + private static List extractKeys(List tuple, List keys, Integer index) { + return tuple[index].subMap(keys).values().toList() + } + + /** + * Return a new tuple without the map in it. + * + * @param tuple A tuple (`List`) channel element. + * @param index The position of the map in the tuple. + * @return A copy of the list without the map. + */ + private static List removeMap(List tuple, Integer index) { + return tuple[0.. keys) { + return tuple.drop(keys.size()) + } + +} diff --git a/subworkflows/local/call_mobile_elements.nf b/subworkflows/local/call_mobile_elements.nf index 7db198be..12e4085e 100644 --- a/subworkflows/local/call_mobile_elements.nf +++ b/subworkflows/local/call_mobile_elements.nf @@ -26,8 +26,11 @@ workflow CALL_MOBILE_ELEMENTS { main: ch_versions = Channel.empty() + // TODO: Fix one sample join error + // Building chromosome channel depending on genome version // TODO: Check how retroseq behaves when running chrY on female samples + // TODO: Would be nicer to read in the fai or dict Channel.of(1..22, 'X', 'Y') .branch { it -> grch37: val_genome_build.equals('GRCh37') @@ -51,8 +54,18 @@ workflow CALL_MOBILE_ELEMENTS { ME_SPLIT_ALIGNMENT ( ch_genome_bam_bai_interval, [[:], []], [] ) ME_INDEX_SPLIT_ALIGNMENT ( ME_SPLIT_ALIGNMENT.out.bam ) + ME_SPLIT_ALIGNMENT.out.bam.toList().dump(tag:'bam') + ME_INDEX_SPLIT_ALIGNMENT.out.bai.toList().dump(tag:'bai') + + //ch_retroseq_input = CustomChannelOperators.joinOnKeys( + // ME_SPLIT_ALIGNMENT.out.bam, + // ME_SPLIT_ALIGNMENT.out.bai, + // ["id", "interval"], 0,0, [failOnMismatch:true, failOnDuplicate:true] + //) + //ch_retroseq_input.dump(tag: 'join', pretty: true) + ME_SPLIT_ALIGNMENT.out.bam - .join(ME_INDEX_SPLIT_ALIGNMENT.out.bai, failOnMismatch:true) + .join(ME_INDEX_SPLIT_ALIGNMENT.out.bai, failOnMismatch:true, failOnDuplicate: true) .set { ch_retroseq_input } ch_me_references @@ -88,12 +101,12 @@ workflow CALL_MOBILE_ELEMENTS { // Concatenate the chromosme vcfs per sample BCFTOOLS_SORT_ME.out.vcf - .map { meta, vcf -> [ meta.findAll { !(it.key in ['interval']) }, vcf ] } + .map { meta, vcf -> [ meta - meta.subMap('interval'), vcf ] } .groupTuple(size: 24) .set { ch_vcfs } TABIX_ME_SPLIT.out.tbi - .map { meta, tbi -> [ meta.findAll { !(it.key in ['interval']) }, tbi ] } + .map { meta, tbi -> [ meta - meta.subMap('interval'), tbi ] } .groupTuple(size: 24) .set { ch_tbis } From 45cc753e086ded70fa73fda6a192f7e5861d6b62 Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 9 Jan 2024 11:00:28 +0100 Subject: [PATCH 206/387] adding missing test file --- conf/test_one_sample.config | 2 + lib/CustomChannelOperators.groovy | 156 --------------------- subworkflows/local/call_mobile_elements.nf | 23 +-- 3 files changed, 6 insertions(+), 175 deletions(-) delete mode 100644 lib/CustomChannelOperators.groovy diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index f39cb40c..81a3dd12 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -41,6 +41,8 @@ params { intervals_wgs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/target_wgs.interval_list" intervals_y = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/targetY.interval_list" known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" + // TODO: Update path for mobile element refs + mobile_element_references = "${projectDir}/assets/mobile_element_references.tsv" ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" score_config_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" diff --git a/lib/CustomChannelOperators.groovy b/lib/CustomChannelOperators.groovy deleted file mode 100644 index f1317a46..00000000 --- a/lib/CustomChannelOperators.groovy +++ /dev/null @@ -1,156 +0,0 @@ -// -// https://github.com/Midnighter/nextflow-utility-services/blob/1f84278c965255fb2bbe290b8d6c9f33f71da26f/lib/CustomChannelOperators.groovy -// -/** - * MIT License - * - * Copyright (c) 2022 Moritz E. Beber - * - * Permission is hereby granted, free of charge, to any person obtaining a copy - * of this software and associated documentation files (the "Software"), to deal - * in the Software without restriction, including without limitation the rights - * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell - * copies of the Software, and to permit persons to whom the Software is - * furnished to do so, subject to the following conditions: - * - * The above copyright notice and this permission notice shall be included in all - * copies or substantial portions of the Software. - * - * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR - * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, - * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE - * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER - * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, - * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE - * SOFTWARE. - */ - -import groovyx.gpars.dataflow.DataflowBroadcast - -/** - * Provide a collection of custom channel operators that go beyond the nextflow default. - * - * @author Moritz E. Beber - * @author Mahesh Binzer-Panchal - */ -class CustomChannelOperators { - - /** - * Join two channels by one or more keys from a map contained in each channel. - * - * The channel elements are assumed to be tuples whose size is at least two. - * Typically, the maps to join by are in the first position of the tuples. - * Please read https://www.nextflow.io/docs/latest/operator.html#join carefully. - * - * @param left The left-hand side channel in the join. - * @param right The right-hand side channel in the join. - * @param keys A list of strings providing the map keys to compare. - * @param leftBy The position of the map in the left channel. - * @param rightBy The position of the map in the right channel. - * @param joinArgs A map of keyword arguments that is passed on to the nextflow join call. - * @return The joined channels with the map in the original position of the left channel, - * followed by all elements of the right channel except for the map. - */ - static DataflowBroadcast joinOnKeys( - DataflowBroadcast left, - DataflowBroadcast right, - List keys, - Integer leftBy, - Integer rightBy, - Map joinArgs - ) { - // Extract desired keys from the left map, located at `leftBy`, and prepend them. - DataflowBroadcast newLeft = left.map { tuple -> - extractKeys(tuple, keys, leftBy) + tuple - } - - // Extract desired keys from the right map, located at `rightBy`, and prepend them. - // Also drop the map itself from the right. - DataflowBroadcast newRight = right.map { tuple -> - extractKeys(tuple, keys, rightBy) + removeMap(tuple, rightBy) - } - - // Set the positions to join on explicitly. - joinArgs.by = 0.. dropKeys(tuple, keys) } - } - - static DataflowBroadcast joinOnKeys( - Map joinArgs, - DataflowBroadcast left, - DataflowBroadcast right, - String key, - Integer leftBy = 0, - Integer rightBy = 0 - ) { - return joinOnKeys(left, right, [key], leftBy, rightBy, joinArgs) - } - - static DataflowBroadcast joinOnKeys( - DataflowBroadcast left, - DataflowBroadcast right, - String key, - Integer leftBy = 0, - Integer rightBy = 0 - ) { - return joinOnKeys(left, right, [key], leftBy, rightBy, [:]) - } - - static DataflowBroadcast joinOnKeys( - Map joinArgs, - DataflowBroadcast left, - DataflowBroadcast right, - List keys, - Integer leftBy = 0, - Integer rightBy = 0 - ) { - return joinOnKeys(left, right, keys, leftBy, rightBy, joinArgs) - } - - static DataflowBroadcast joinOnKeys( - DataflowBroadcast left, - DataflowBroadcast right, - List keys, - Integer leftBy = 0, - Integer rightBy = 0 - ) { - return joinOnKeys(left, right, keys, leftBy, rightBy, [:]) - } - - /** - * Extract values from a map at given position with given keys. - * - * @param tuple A tuple (`List`) channel element. - * @param keys A list of strings denoting keys in the map. - * @param index The position of the map in the tuple. - * @return The list of values extracted from the map. - */ - private static List extractKeys(List tuple, List keys, Integer index) { - return tuple[index].subMap(keys).values().toList() - } - - /** - * Return a new tuple without the map in it. - * - * @param tuple A tuple (`List`) channel element. - * @param index The position of the map in the tuple. - * @return A copy of the list without the map. - */ - private static List removeMap(List tuple, Integer index) { - return tuple[0.. keys) { - return tuple.drop(keys.size()) - } - -} diff --git a/subworkflows/local/call_mobile_elements.nf b/subworkflows/local/call_mobile_elements.nf index 12e4085e..45432ae6 100644 --- a/subworkflows/local/call_mobile_elements.nf +++ b/subworkflows/local/call_mobile_elements.nf @@ -26,8 +26,6 @@ workflow CALL_MOBILE_ELEMENTS { main: ch_versions = Channel.empty() - // TODO: Fix one sample join error - // Building chromosome channel depending on genome version // TODO: Check how retroseq behaves when running chrY on female samples // TODO: Would be nicer to read in the fai or dict @@ -54,16 +52,6 @@ workflow CALL_MOBILE_ELEMENTS { ME_SPLIT_ALIGNMENT ( ch_genome_bam_bai_interval, [[:], []], [] ) ME_INDEX_SPLIT_ALIGNMENT ( ME_SPLIT_ALIGNMENT.out.bam ) - ME_SPLIT_ALIGNMENT.out.bam.toList().dump(tag:'bam') - ME_INDEX_SPLIT_ALIGNMENT.out.bai.toList().dump(tag:'bai') - - //ch_retroseq_input = CustomChannelOperators.joinOnKeys( - // ME_SPLIT_ALIGNMENT.out.bam, - // ME_SPLIT_ALIGNMENT.out.bai, - // ["id", "interval"], 0,0, [failOnMismatch:true, failOnDuplicate:true] - //) - //ch_retroseq_input.dump(tag: 'join', pretty: true) - ME_SPLIT_ALIGNMENT.out.bam .join(ME_INDEX_SPLIT_ALIGNMENT.out.bai, failOnMismatch:true, failOnDuplicate: true) .set { ch_retroseq_input } @@ -99,7 +87,7 @@ workflow CALL_MOBILE_ELEMENTS { BCFTOOLS_SORT_ME ( BCFTOOLS_REHEADER_ME.out.vcf ) TABIX_ME_SPLIT ( BCFTOOLS_SORT_ME.out.vcf ) - // Concatenate the chromosme vcfs per sample + // Concatenate the chromosome vcfs to sample vcfs BCFTOOLS_SORT_ME.out.vcf .map { meta, vcf -> [ meta - meta.subMap('interval'), vcf ] } .groupTuple(size: 24) @@ -129,10 +117,6 @@ workflow CALL_MOBILE_ELEMENTS { SVDB_MERGE_ME ( ch_svdb_merge_me_input, [] ) TABIX_ME ( SVDB_MERGE_ME.out.vcf ) - SVDB_MERGE_ME.out.vcf - .join(TABIX_ME.out.tbi) - .set { ch_me_vcf } - ch_versions = ch_versions.mix(ME_SPLIT_ALIGNMENT.out.versions).first() ch_versions = ch_versions.mix(ME_INDEX_SPLIT_ALIGNMENT.out.versions).first() ch_versions = ch_versions.mix(RETROSEQ_DISCOVER.out.versions).first() @@ -145,6 +129,7 @@ workflow CALL_MOBILE_ELEMENTS { ch_versions = ch_versions.mix(TABIX_ME.out.versions) emit: - me_vcf = ch_me_vcf // channel: [ val(meta), path(vcf), path(tbi) ] - versions = ch_versions // channel: [ path(versions.yml) ] + vcf = SVDB_MERGE_ME.out.vcf // channel: [ val(meta), path(vcf) ] + tbi = TABIX_ME.out.tbi // channel: [ val(meta), path(tbi) ] + versions = ch_versions // channel: [ path(versions.yml) ] } From 08b7203e31d9b81833d38fdbaf5bbac89c173f04 Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 9 Jan 2024 11:06:28 +0100 Subject: [PATCH 207/387] fix config indentation --- conf/modules/call_mobile_elements.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules/call_mobile_elements.config b/conf/modules/call_mobile_elements.config index 788404a3..fabc6b94 100644 --- a/conf/modules/call_mobile_elements.config +++ b/conf/modules/call_mobile_elements.config @@ -62,7 +62,7 @@ process { ] } - withName: '.*CALL_MOBILE_ELEMENTS:TABIX_ME' { + withName: '.*CALL_MOBILE_ELEMENTS:TABIX_ME' { publishDir = [ path: { "${params.outdir}/call_mobile_elements" }, mode: params.publish_dir_mode, From 3be46420032c2018ee47842db4035e2797e908fe Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 9 Jan 2024 16:23:01 +0100 Subject: [PATCH 208/387] fixing around with test data --- CITATIONS.md | 4 ++++ README.md | 4 ++++ assets/grch37_g1k_alu.bed | 0 assets/grch37_g1k_herv.bed | 0 assets/grch37_g1k_l1.bed | 0 assets/grch37_g1k_sva.bed | 0 assets/mobile_element_references.tsv | 5 ----- conf/test.config | 4 ++-- conf/test_one_sample.config | 4 ++-- conf/test_sentieon.config | 2 ++ main.nf | 1 - subworkflows/local/call_mobile_elements.nf | 21 +++++++-------------- workflows/raredisease.nf | 20 +++++++++----------- 13 files changed, 30 insertions(+), 35 deletions(-) delete mode 100644 assets/grch37_g1k_alu.bed delete mode 100644 assets/grch37_g1k_herv.bed delete mode 100644 assets/grch37_g1k_l1.bed delete mode 100644 assets/grch37_g1k_sva.bed delete mode 100644 assets/mobile_element_references.tsv diff --git a/CITATIONS.md b/CITATIONS.md index b20f58ac..f9973ae3 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -100,6 +100,10 @@ > Okonechnikov K, Conesa A, García-Alcalde F. Qualimap 2: advanced multi-sample quality control for high-throughput sequencing data. Bioinformatics. 2016;32(2):292-294. doi:10.1093/bioinformatics/btv566 +- [RetroSeq](https://academic.oup.com/bioinformatics/article/29/3/389/257479) + + > Thomas M. Keane, Kim Wong, David J. Adams, RetroSeq: transposable element discovery from next-generation sequencing data. Bioinformatics.2013 Feb 1;29(3):389-90. doi: 10.1093/bioinformatics/bts697 + - [rhocall](https://github.com/dnil/rhocall) - [Sentieon DNAscope](https://www.biorxiv.org/content/10.1101/2022.05.20.492556v1.abstract) diff --git a/README.md b/README.md index 3c36427a..ff227da8 100644 --- a/README.md +++ b/README.md @@ -93,6 +93,10 @@ On release, automated continuous integration tests run the pipeline on a full-si - [Expansion Hunter](https://github.com/Illumina/ExpansionHunter) - [Stranger](https://github.com/Clinical-Genomics/stranger) +**9. Variant calling - mobile elements:** + +- [RetroSeq](https://github.com/tk2/RetroSeq) + **9. Rank variants - SV and SNV:** - [GENMOD](https://github.com/Clinical-Genomics/genmod) diff --git a/assets/grch37_g1k_alu.bed b/assets/grch37_g1k_alu.bed deleted file mode 100644 index e69de29b..00000000 diff --git a/assets/grch37_g1k_herv.bed b/assets/grch37_g1k_herv.bed deleted file mode 100644 index e69de29b..00000000 diff --git a/assets/grch37_g1k_l1.bed b/assets/grch37_g1k_l1.bed deleted file mode 100644 index e69de29b..00000000 diff --git a/assets/grch37_g1k_sva.bed b/assets/grch37_g1k_sva.bed deleted file mode 100644 index e69de29b..00000000 diff --git a/assets/mobile_element_references.tsv b/assets/mobile_element_references.tsv deleted file mode 100644 index e5a804ff..00000000 --- a/assets/mobile_element_references.tsv +++ /dev/null @@ -1,5 +0,0 @@ -type path -L1 ./assets/grch37_g1k_l1.bed -SVA ./assets/grch37_g1k_sva.bed -ALU ./assets/grch37_g1k_alu.bed -HERV ./assets/grch37_g1k_herv.bed diff --git a/conf/test.config b/conf/test.config index e691a70d..d43f319b 100644 --- a/conf/test.config +++ b/conf/test.config @@ -36,13 +36,13 @@ params { // Genome references fasta = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reference.fasta" + fai = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reference.fasta.fai" genome = 'GRCh37' gnomad_af = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/gnomad_reformated.tab.gz" intervals_wgs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/target_wgs.interval_list" intervals_y = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/targetY.interval_list" known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" - // TODO: Update path for mobile element refs - mobile_element_references = "${projectDir}/assets/mobile_element_references.tsv" + mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" score_config_mt = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index 81a3dd12..dc99e145 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -36,13 +36,13 @@ params { // Genome references fasta = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reference.fasta" + fai = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reference.fasta.fai" genome = 'GRCh37' gnomad_af = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/gnomad_reformated.tab.gz" intervals_wgs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/target_wgs.interval_list" intervals_y = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/targetY.interval_list" known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" - // TODO: Update path for mobile element refs - mobile_element_references = "${projectDir}/assets/mobile_element_references.tsv" + mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" score_config_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" diff --git a/conf/test_sentieon.config b/conf/test_sentieon.config index 64618d12..aca4b755 100644 --- a/conf/test_sentieon.config +++ b/conf/test_sentieon.config @@ -31,11 +31,13 @@ params { // Genome references fasta = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reference.fasta" + fai = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reference.fasta.fai" genome = 'GRCh37' gnomad_af = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/gnomad_reformated.tab.gz" intervals_wgs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/target_wgs.interval_list" intervals_y = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/targetY.interval_list" known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" + mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" score_config_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" diff --git a/main.nf b/main.nf index 5b0bb182..0ebf4e51 100644 --- a/main.nf +++ b/main.nf @@ -16,7 +16,6 @@ nextflow.enable.dsl = 2 GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// TODO: Are the genome config used? params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') params.fai = WorkflowMain.getGenomeAttribute(params, 'fai') params.bwa = WorkflowMain.getGenomeAttribute(params, 'bwa') diff --git a/subworkflows/local/call_mobile_elements.nf b/subworkflows/local/call_mobile_elements.nf index 45432ae6..c77a11d9 100644 --- a/subworkflows/local/call_mobile_elements.nf +++ b/subworkflows/local/call_mobile_elements.nf @@ -3,13 +3,13 @@ // include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_ME } from '../../modules/nf-core/bcftools/reheader/main' -include { BCFTOOLS_CONCAT as BCFTOOLS_CONCAT_ME } from '../../modules/nf-core/bcftools/concat/main' -include { BCFTOOLS_SORT as BCFTOOLS_SORT_ME } from '../../modules/nf-core/bcftools/sort/main' +include { BCFTOOLS_CONCAT as BCFTOOLS_CONCAT_ME } from '../../modules/nf-core/bcftools/concat/main' +include { BCFTOOLS_SORT as BCFTOOLS_SORT_ME } from '../../modules/nf-core/bcftools/sort/main' include { RETROSEQ_CALL as RETROSEQ_CALL } from '../../modules/local/retroseq/call/main' include { RETROSEQ_DISCOVER as RETROSEQ_DISCOVER } from '../../modules/local/retroseq/discover/main' include { SAMTOOLS_INDEX as ME_INDEX_SPLIT_ALIGNMENT } from '../../modules/nf-core/samtools/index/main' include { SAMTOOLS_VIEW as ME_SPLIT_ALIGNMENT } from '../../modules/nf-core/samtools/view/main' -include { TABIX_TABIX as TABIX_ME } from '../../modules/nf-core/tabix/tabix/main' +include { TABIX_TABIX as TABIX_ME } from '../../modules/nf-core/tabix/tabix/main' include { TABIX_TABIX as TABIX_ME_SPLIT } from '../../modules/nf-core/tabix/tabix/main' include { SVDB_MERGE as SVDB_MERGE_ME } from '../../modules/nf-core/svdb/merge/main' @@ -26,17 +26,10 @@ workflow CALL_MOBILE_ELEMENTS { main: ch_versions = Channel.empty() - // Building chromosome channel depending on genome version - // TODO: Check how retroseq behaves when running chrY on female samples - // TODO: Would be nicer to read in the fai or dict - Channel.of(1..22, 'X', 'Y') - .branch { it -> - grch37: val_genome_build.equals('GRCh37') - return [it.toString()] - grch38: val_genome_build.equals('GRCh38') - return ['chr' + it.toString()] - }.set{ ch_chr_genome } - ch_chr_genome.grch37.mix(ch_chr_genome.grch38) + // Building chromosome channels based on fasta index + ch_genome_fai + .splitCsv(sep: "\t", elem: 1, limit: 25) + .map { meta, fai -> [ fai.first() ] } .set { ch_chr } // Building one bam channel per chromosome and adding interval diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 0016a154..5fafd6a7 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -293,9 +293,7 @@ workflow RAREDISEASE { // SV caller priority if (params.skip_germlinecnvcaller) { - // TODO: Uncomment and remove - ch_svcaller_priority = Channel.value(["tiddit", "manta"]) - //ch_svcaller_priority = Channel.value(["tiddit", "manta", "cnvnator"]) + ch_svcaller_priority = Channel.value(["tiddit", "manta", "cnvnator"]) } else { ch_svcaller_priority = Channel.value(["tiddit", "manta", "gcnvcaller", "cnvnator"]) } @@ -356,14 +354,14 @@ workflow RAREDISEASE { // // EXPANSIONHUNTER AND STRANGER // - CALL_REPEAT_EXPANSIONS ( - ch_mapped.genome_bam_bai, - ch_variant_catalog, - ch_case_info, - ch_genome_fasta, - ch_genome_fai - ) - ch_versions = ch_versions.mix(CALL_REPEAT_EXPANSIONS.out.versions) + //CALL_REPEAT_EXPANSIONS ( + // ch_mapped.genome_bam_bai, + // ch_variant_catalog, + // ch_case_info, + // ch_genome_fasta, + // ch_genome_fai + //) + //ch_versions = ch_versions.mix(CALL_REPEAT_EXPANSIONS.out.versions) // // SNV CALLING From 4bbb604114b628aa1239fc41f5236b0b1fd4e5a8 Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 9 Jan 2024 16:27:43 +0100 Subject: [PATCH 209/387] update readme --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index ff227da8..ebb4b95a 100644 --- a/README.md +++ b/README.md @@ -97,7 +97,7 @@ On release, automated continuous integration tests run the pipeline on a full-si - [RetroSeq](https://github.com/tk2/RetroSeq) -**9. Rank variants - SV and SNV:** +**10. Rank variants - SV and SNV:** - [GENMOD](https://github.com/Clinical-Genomics/genmod) From e50756fde73afd0ffdb7a84b2efd5c1cac2d21bd Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 9 Jan 2024 16:29:39 +0100 Subject: [PATCH 210/387] uncommenting lines --- workflows/raredisease.nf | 16 ++++++++-------- 1 file changed, 8 insertions(+), 8 deletions(-) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 5fafd6a7..cb23fe7d 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -354,14 +354,14 @@ workflow RAREDISEASE { // // EXPANSIONHUNTER AND STRANGER // - //CALL_REPEAT_EXPANSIONS ( - // ch_mapped.genome_bam_bai, - // ch_variant_catalog, - // ch_case_info, - // ch_genome_fasta, - // ch_genome_fai - //) - //ch_versions = ch_versions.mix(CALL_REPEAT_EXPANSIONS.out.versions) + CALL_REPEAT_EXPANSIONS ( + ch_mapped.genome_bam_bai, + ch_variant_catalog, + ch_case_info, + ch_genome_fasta, + ch_genome_fai + ) + ch_versions = ch_versions.mix(CALL_REPEAT_EXPANSIONS.out.versions) // // SNV CALLING From e048f9f0976ced4521f36b4c2d0b8898ef4daa01 Mon Sep 17 00:00:00 2001 From: Jakob Willforss Date: Tue, 9 Jan 2024 16:30:55 +0100 Subject: [PATCH 211/387] Tabix prior to BCF view --- .../variant_calling/call_sv_germlinecnvcaller.nf | 12 +++++++++++- 1 file changed, 11 insertions(+), 1 deletion(-) diff --git a/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf b/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf index a9420a62..4e27b14c 100644 --- a/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf +++ b/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf @@ -7,6 +7,7 @@ include { GATK4_DETERMINEGERMLINECONTIGPLOIDY } from '../../../modules/nf-core/g include { GATK4_GERMLINECNVCALLER } from '../../../modules/nf-core/gatk4/germlinecnvcaller/main.nf' include { GATK4_POSTPROCESSGERMLINECNVCALLS } from '../../../modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf' include { BCFTOOLS_VIEW } from '../../../modules/nf-core/bcftools/view/main' +include { TABIX_TABIX } from '../../../modules/nf-core/tabix/tabix/main' workflow CALL_SV_GERMLINECNVCALLER { take: @@ -48,13 +49,22 @@ workflow CALL_SV_GERMLINECNVCALLER { GATK4_POSTPROCESSGERMLINECNVCALLS ( ch_postproc_in ) + // GATK4_POSTPROCESSGERMLINECNVCALLS.out.segments.view() + + TABIX_TABIX(GATK4_POSTPROCESSGERMLINECNVCALLS.out.segments) + TABIX_TABIX.out.tbi.view() + GATK4_POSTPROCESSGERMLINECNVCALLS.out.segments + .join(TABIX_TABIX.out.tbi, failOnMismatch:true) + .set {ch_segments_in} + ch_segments_in.view() // Filter out reference only (0/0) segments - BCFTOOLS_VIEW ( GATK4_POSTPROCESSGERMLINECNVCALLS.out.segments, [], [], [] ) + BCFTOOLS_VIEW (ch_segments_in , [], [], [] ) ch_versions = ch_versions.mix(GATK4_COLLECTREADCOUNTS.out.versions) ch_versions = ch_versions.mix(GATK4_DETERMINEGERMLINECONTIGPLOIDY.out.versions) ch_versions = ch_versions.mix(GATK4_GERMLINECNVCALLER.out.versions) ch_versions = ch_versions.mix(GATK4_POSTPROCESSGERMLINECNVCALLS.out.versions) + ch_versions = ch_versions.mix(TABIX_TABIX.out.versions) ch_versions = ch_versions.mix(BCFTOOLS_VIEW.out.versions) emit: From 9726f06cde5d0c892ea2a22565fdfd532e5d3b5b Mon Sep 17 00:00:00 2001 From: Jakob Willforss Date: Tue, 9 Jan 2024 16:45:51 +0100 Subject: [PATCH 212/387] Fix BNFTOOLS filtering config --- conf/modules/call_sv_germlinecnvcaller.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules/call_sv_germlinecnvcaller.config b/conf/modules/call_sv_germlinecnvcaller.config index c41251ae..6685dc6c 100644 --- a/conf/modules/call_sv_germlinecnvcaller.config +++ b/conf/modules/call_sv_germlinecnvcaller.config @@ -38,7 +38,7 @@ process { ext.prefix = { "${meta.id}_${model.simpleName}" } } - withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_VIEW' { + withName: '.*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:BCFTOOLS_VIEW' { ext.prefix = { "${meta.id}_gatkcnv_segments_reffiltered" } ext.args = { '--output-type z --exclude "N_ALT = 0" ' } } From 8cacd8f99960acd6040ad0b6246a81083539ade5 Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 9 Jan 2024 16:54:40 +0100 Subject: [PATCH 213/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index ce1a867b..6a0e09ce 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -19,6 +19,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - ngsbits samplegender to check sex [#453](https://github.com/nf-core/raredisease/pull/453) - New workflow for generating cgh files from SV vcfs for interpretation in the CytosSure interpretation software. Turned off by default [#456](https://github.com/nf-core/raredisease/pull/456/) - Fastp to do adapter trimming. It can be skipped using `--skip_fastp` [#457](https://github.com/nf-core/raredisease/pull/457) +- New workflow for calling insertion of mobile elements [#440](https://github.com/nf-core/raredisease/pull/440) ### `Changed` From db50a4ba1e3bf9e19ee6535793bb442476d5c72f Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 9 Jan 2024 17:40:23 +0100 Subject: [PATCH 214/387] updating and removing patch for vcf2cytosure --- modules.json | 2 +- modules/nf-core/vcf2cytosure/environment.yml | 1 + modules/nf-core/vcf2cytosure/meta.yml | 4 +- .../nf-core/vcf2cytosure/tests/main.nf.test | 73 +++++++++++++++++++ .../vcf2cytosure/tests/main.nf.test.snap | 44 +++++++++++ modules/nf-core/vcf2cytosure/tests/tags.yml | 2 + .../nf-core/vcf2cytosure/vcf2cytosure.diff | 23 ------ 7 files changed, 123 insertions(+), 26 deletions(-) create mode 100644 modules/nf-core/vcf2cytosure/tests/main.nf.test create mode 100644 modules/nf-core/vcf2cytosure/tests/main.nf.test.snap create mode 100644 modules/nf-core/vcf2cytosure/tests/tags.yml delete mode 100644 modules/nf-core/vcf2cytosure/vcf2cytosure.diff diff --git a/modules.json b/modules.json index e0a92fa6..c98929d7 100644 --- a/modules.json +++ b/modules.json @@ -475,7 +475,7 @@ }, "vcf2cytosure": { "branch": "master", - "git_sha": "2dba354d7c0a43a1e6d6ce26f408b4936d4ed619", + "git_sha": "8694793954175ddd3001ddfbc0e36782a674d8a7", "installed_by": ["modules"], "patch": "modules/nf-core/vcf2cytosure/vcf2cytosure.diff" }, diff --git a/modules/nf-core/vcf2cytosure/environment.yml b/modules/nf-core/vcf2cytosure/environment.yml index 30e0542f..d4bf9959 100644 --- a/modules/nf-core/vcf2cytosure/environment.yml +++ b/modules/nf-core/vcf2cytosure/environment.yml @@ -1,3 +1,4 @@ +name: vcf2cytosure channels: - conda-forge - bioconda diff --git a/modules/nf-core/vcf2cytosure/meta.yml b/modules/nf-core/vcf2cytosure/meta.yml index 39badd4c..2430f1ae 100644 --- a/modules/nf-core/vcf2cytosure/meta.yml +++ b/modules/nf-core/vcf2cytosure/meta.yml @@ -13,7 +13,7 @@ tools: homepage: "https://github.com/NBISweden/vcf2cytosure" documentation: "https://github.com/NBISweden/vcf2cytosure" tool_dev_url: "https://github.com/NBISweden/vcf2cytosure" - licence: "['MIT']" + licence: ["MIT"] input: - meta: @@ -53,7 +53,7 @@ input: VCF file with SNVs to calculate probe coverage, not compatible with coverage_bed pattern: "*.{vcf,vcf.gz}" - - blacklilst_bed: + - blacklist_bed: type: file description: Bed file with regions to exclude pattern: "*.bed" diff --git a/modules/nf-core/vcf2cytosure/tests/main.nf.test b/modules/nf-core/vcf2cytosure/tests/main.nf.test new file mode 100644 index 00000000..d017cb0e --- /dev/null +++ b/modules/nf-core/vcf2cytosure/tests/main.nf.test @@ -0,0 +1,73 @@ +nextflow_process { + + name "Test Process VCF2CYTOSURE" + script "../main.nf" + process "VCF2CYTOSURE" + + tag "modules" + tag "modules_nfcore" + tag "vcf2cytosure" + + test("homo sapiens - vcf - bed") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + [ file(params.test_data['homo_sapiens']['illumina']['na24385_chr22_sv_vcf'], checkIfExists: true) ] + ] + input[1] = [ + [ id:'test' ], // meta map + [ file(params.test_data['homo_sapiens']['illumina']['na24385_chr22_coverage'], checkIfExists: true) ] + ] + input[2] = [ [:], [] ] + input[3] = [ [:], [] ] + input[4] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.cgh).match("cgh") }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("homo sapiens - vcf - bed - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + [ file(params.test_data['homo_sapiens']['illumina']['na24385_chr22_sv_vcf'], checkIfExists: true) ] + ] + input[1] = [ + [ id:'test' ], // meta map + [ file(params.test_data['homo_sapiens']['illumina']['na24385_chr22_coverage'], checkIfExists: true) ] + ] + input[2] = [ [:], [] ] + input[3] = [ [:], [] ] + input[4] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.cgh).match("cgh - stub") }, + { assert snapshot(process.out.versions).match("versions - stub") } + ) + } + + } + +} diff --git a/modules/nf-core/vcf2cytosure/tests/main.nf.test.snap b/modules/nf-core/vcf2cytosure/tests/main.nf.test.snap new file mode 100644 index 00000000..0a0fae29 --- /dev/null +++ b/modules/nf-core/vcf2cytosure/tests/main.nf.test.snap @@ -0,0 +1,44 @@ +{ + "cgh": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.cgh:md5,fc3f1ffac5b797881d992994d5d56550" + ] + ] + ], + "timestamp": "2023-12-21T18:33:25.202806" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,a1850e66d57cee0b98adb056c1dc3ebb" + ] + ], + "timestamp": "2023-12-21T18:33:25.205826" + }, + "cgh - stub": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.cgh:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "timestamp": "2023-12-21T18:38:06.660655" + }, + "versions - stub": { + "content": [ + [ + "versions.yml:md5,a1850e66d57cee0b98adb056c1dc3ebb" + ] + ], + "timestamp": "2023-12-21T18:38:06.663571" + } +} \ No newline at end of file diff --git a/modules/nf-core/vcf2cytosure/tests/tags.yml b/modules/nf-core/vcf2cytosure/tests/tags.yml new file mode 100644 index 00000000..88ff6038 --- /dev/null +++ b/modules/nf-core/vcf2cytosure/tests/tags.yml @@ -0,0 +1,2 @@ +vcf2cytosure: + - "modules/nf-core/vcf2cytosure/**" diff --git a/modules/nf-core/vcf2cytosure/vcf2cytosure.diff b/modules/nf-core/vcf2cytosure/vcf2cytosure.diff deleted file mode 100644 index f80980da..00000000 --- a/modules/nf-core/vcf2cytosure/vcf2cytosure.diff +++ /dev/null @@ -1,23 +0,0 @@ -Changes in module 'nf-core/vcf2cytosure' ---- modules/nf-core/vcf2cytosure/main.nf -+++ modules/nf-core/vcf2cytosure/main.nf -@@ -12,7 +12,7 @@ - tuple val(meta2), path(coverage_bed) - tuple val(meta3), path(cns) - tuple val(meta4), path(snv_vcf) -- path blacklist_bed -+ path(blacklist_bed) - - output: - tuple val(meta), path("*.cgh"), emit: cgh -@@ -56,7 +56,7 @@ - def prefix = task.ext.prefix ?: "${meta.id}" - - """ -- touch ${prefix}.xml -+ touch ${prefix}.cgh - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - -************************************************************ From a68e499f403aa164b179239057ad6df784973dde Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 9 Jan 2024 17:49:10 +0100 Subject: [PATCH 215/387] updating and removing patch for tiddit cov --- modules.json | 2 +- modules/nf-core/tiddit/cov/environment.yml | 7 + modules/nf-core/tiddit/cov/main.nf | 2 +- modules/nf-core/tiddit/cov/meta.yml | 7 +- modules/nf-core/tiddit/cov/tests/main.nf.test | 160 ++++++++++++++++++ .../tiddit/cov/tests/main.nf.test.snap | 74 ++++++++ .../nf-core/tiddit/cov/tests/nextflow.config | 8 + modules/nf-core/tiddit/cov/tests/tags.yml | 2 + modules/nf-core/tiddit/cov/tiddit-cov.diff | 26 --- 9 files changed, 257 insertions(+), 31 deletions(-) create mode 100644 modules/nf-core/tiddit/cov/environment.yml create mode 100644 modules/nf-core/tiddit/cov/tests/main.nf.test create mode 100644 modules/nf-core/tiddit/cov/tests/main.nf.test.snap create mode 100644 modules/nf-core/tiddit/cov/tests/nextflow.config create mode 100644 modules/nf-core/tiddit/cov/tests/tags.yml delete mode 100644 modules/nf-core/tiddit/cov/tiddit-cov.diff diff --git a/modules.json b/modules.json index c98929d7..ea3913c2 100644 --- a/modules.json +++ b/modules.json @@ -449,7 +449,7 @@ }, "tiddit/cov": { "branch": "master", - "git_sha": "1c90a501d102b800c27697f5ef39a6e217ab1915", + "git_sha": "ae07bd3d3e229ca82bd94531648736d44367a391", "installed_by": ["modules"], "patch": "modules/nf-core/tiddit/cov/tiddit-cov.diff" }, diff --git a/modules/nf-core/tiddit/cov/environment.yml b/modules/nf-core/tiddit/cov/environment.yml new file mode 100644 index 00000000..6b024492 --- /dev/null +++ b/modules/nf-core/tiddit/cov/environment.yml @@ -0,0 +1,7 @@ +name: tiddit_cov +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::tiddit=3.6.1 diff --git a/modules/nf-core/tiddit/cov/main.nf b/modules/nf-core/tiddit/cov/main.nf index e8cafaa5..20d4720e 100644 --- a/modules/nf-core/tiddit/cov/main.nf +++ b/modules/nf-core/tiddit/cov/main.nf @@ -2,7 +2,7 @@ process TIDDIT_COV { tag "$meta.id" label 'process_low' - conda "bioconda::tiddit=3.6.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/tiddit:3.6.1--py38h24c8ff8_0' : 'biocontainers/tiddit:3.6.1--py38h24c8ff8_0' }" diff --git a/modules/nf-core/tiddit/cov/meta.yml b/modules/nf-core/tiddit/cov/meta.yml index 9cc586a9..c28546cc 100644 --- a/modules/nf-core/tiddit/cov/meta.yml +++ b/modules/nf-core/tiddit/cov/meta.yml @@ -12,7 +12,6 @@ tools: documentation: https://github.com/SciLifeLab/TIDDIT/blob/master/README.md doi: "10.12688/f1000research.11168.1" licence: ["GPL v3"] - input: - meta: type: map @@ -34,7 +33,6 @@ input: Reference genome file. Only needed when passing in CRAM instead of BAM. If not using CRAM, please pass an empty file instead. pattern: "*.fasta" - output: - meta: type: map @@ -43,7 +41,7 @@ output: e.g. [ id:'test', single_end:false ] - cov: type: file - description: The coverage of different regions. Optional. + description: The coverage of different regions in bed format. Optional. pattern: "*.bed" - wig: type: file @@ -56,3 +54,6 @@ output: authors: - "@projectoriented" - "@ramprasadn" +maintainers: + - "@projectoriented" + - "@ramprasadn" diff --git a/modules/nf-core/tiddit/cov/tests/main.nf.test b/modules/nf-core/tiddit/cov/tests/main.nf.test new file mode 100644 index 00000000..72746648 --- /dev/null +++ b/modules/nf-core/tiddit/cov/tests/main.nf.test @@ -0,0 +1,160 @@ +nextflow_process { + + name "Test Process TIDDIT_COV" + script "../main.nf" + process "TIDDIT_COV" + + tag "modules" + tag "modules_nfcore" + tag "tiddit" + tag "tiddit/cov" + + test("homo_sapiens - cram - bed") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true) + ] + + input[1] = [ + [:], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.cov).match("cov") }, + { assert process.out.cov[0][1] ==~ ".*/test.bed" }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("homo_sapiens - bam - bed") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) + ] + input[1] = [ [:], [] ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.cov).match("cov") }, + { assert process.out.cov[0][1] ==~ ".*/test.bed" }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("homo_sapiens - cram - wig") { + + config "./nextflow.config" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true) + ] + + input[1] = [ + [:], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.wig).match("wig") }, + { assert process.out.wig[0][1] ==~ ".*/test.wig" }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("homo_sapiens - bam - wig") { + + config "./nextflow.config" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) + ] + + input[1] = [ [:], [] ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.wig).match("wig") }, + { assert process.out.wig[0][1] ==~ ".*/test.wig" }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + + test("homo_sapiens - stub") { + + options "-stub" + + when { + process { + """ + + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true) + ] + + input[1] = [ + [:], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.cov).match("cov - stub") }, + { assert process.out.cov[0][1] ==~ ".*/test.bed" }, + { assert snapshot(process.out.wig).match("wig - stub") }, + { assert process.out.wig[0][1] ==~ ".*/test.wig" }, + { assert snapshot(process.out.versions).match("versions - stub") } + ) + } + + } + +} diff --git a/modules/nf-core/tiddit/cov/tests/main.nf.test.snap b/modules/nf-core/tiddit/cov/tests/main.nf.test.snap new file mode 100644 index 00000000..dcd24edc --- /dev/null +++ b/modules/nf-core/tiddit/cov/tests/main.nf.test.snap @@ -0,0 +1,74 @@ +{ + "wig": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.wig:md5,7c46b6ef30574acdce0ad854f40126ae" + ] + ] + ], + "timestamp": "2023-12-22T11:24:06.649602" + }, + "wig - stub": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.wig:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "timestamp": "2023-12-22T11:30:00.831686" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,49dfdb5a33d1c11db488fb02d1c95c5a" + ] + ], + "timestamp": "2023-12-22T11:23:58.227251" + }, + "cov": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.bed:md5,caf0742f2d4a6f713ceb85268dd48c14" + ] + ] + ], + "timestamp": "2023-12-22T11:23:58.220115" + }, + "versions - stub": { + "content": [ + [ + "versions.yml:md5,49dfdb5a33d1c11db488fb02d1c95c5a" + ] + ], + "timestamp": "2023-12-22T11:30:00.834854" + }, + "cov - stub": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.bed:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "timestamp": "2023-12-22T11:30:00.826974" + } +} \ No newline at end of file diff --git a/modules/nf-core/tiddit/cov/tests/nextflow.config b/modules/nf-core/tiddit/cov/tests/nextflow.config new file mode 100644 index 00000000..f83bd699 --- /dev/null +++ b/modules/nf-core/tiddit/cov/tests/nextflow.config @@ -0,0 +1,8 @@ +process { + + + withName: TIDDIT_COV { + ext.args = '-w' + } + +} diff --git a/modules/nf-core/tiddit/cov/tests/tags.yml b/modules/nf-core/tiddit/cov/tests/tags.yml new file mode 100644 index 00000000..d5831f1d --- /dev/null +++ b/modules/nf-core/tiddit/cov/tests/tags.yml @@ -0,0 +1,2 @@ +tiddit/cov: + - "modules/nf-core/tiddit/cov/**" diff --git a/modules/nf-core/tiddit/cov/tiddit-cov.diff b/modules/nf-core/tiddit/cov/tiddit-cov.diff deleted file mode 100644 index acf8216f..00000000 --- a/modules/nf-core/tiddit/cov/tiddit-cov.diff +++ /dev/null @@ -1,26 +0,0 @@ -Changes in module 'nf-core/tiddit/cov' ---- modules/nf-core/tiddit/cov/meta.yml -+++ modules/nf-core/tiddit/cov/meta.yml -@@ -44,7 +44,7 @@ - - cov: - type: file - description: The coverage of different regions. Optional. -- pattern: "*.tab" -+ pattern: "*.bed" - - wig: - type: file - description: The coverage of different regions in WIG format. Optional. - ---- modules/nf-core/tiddit/cov/main.nf -+++ modules/nf-core/tiddit/cov/main.nf -@@ -41,7 +41,7 @@ - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.wig -- touch ${prefix}.tab -+ touch ${prefix}.bed - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - -************************************************************ From 630b347348d26b3749a95e2e5e03c3eacea7f346 Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 9 Jan 2024 18:02:15 +0100 Subject: [PATCH 216/387] trigging rerun of ci - adding blank line --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index ce1a867b..3f03698a 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -5,6 +5,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## v1.2.0 - Genie [xxxx-xx-xx] + ### `Added` - Use `nf-validation` plugin for parameter and samplesheet validation [#386](https://github.com/nf-core/raredisease/pull/386) From 54a20a3ca5abf7d8d947cb2ea75ccc33c112632a Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 9 Jan 2024 18:04:18 +0100 Subject: [PATCH 217/387] trigging rerun of ci - removing blank line --- CHANGELOG.md | 1 - 1 file changed, 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 3f03698a..ce1a867b 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -5,7 +5,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## v1.2.0 - Genie [xxxx-xx-xx] - ### `Added` - Use `nf-validation` plugin for parameter and samplesheet validation [#386](https://github.com/nf-core/raredisease/pull/386) From ec5bddc363ce4da24148a26b930447c54f149933 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 10 Jan 2024 05:49:22 +0100 Subject: [PATCH 218/387] found in deepvariant --- assets/foundin.hdr | 1 + conf/modules/call_snv_deepvariant.config | 8 ++++ conf/test_one_sample.config | 1 + lib/WorkflowRaredisease.groovy | 14 ++++++ subworkflows/local/call_snv.nf | 4 +- .../variant_calling/call_snv_deepvariant.nf | 45 ++++++++++++++----- workflows/raredisease.nf | 2 + 7 files changed, 63 insertions(+), 12 deletions(-) create mode 100644 assets/foundin.hdr diff --git a/assets/foundin.hdr b/assets/foundin.hdr new file mode 100644 index 00000000..9b38b87f --- /dev/null +++ b/assets/foundin.hdr @@ -0,0 +1 @@ +##INFO= diff --git a/conf/modules/call_snv_deepvariant.config b/conf/modules/call_snv_deepvariant.config index 329bc15f..fdd7ece6 100644 --- a/conf/modules/call_snv_deepvariant.config +++ b/conf/modules/call_snv_deepvariant.config @@ -39,4 +39,12 @@ process { ext.prefix = { "${meta.id}_split_rmdup" } } + withName: '.*CALL_SNV_DEEPVARIANT:ZIP_TABIX_VARCALLERBED' { + ext.args2 = '-s 1 -b 2 -e 3' + } + + withName: '.*CALL_SNV_DEEPVARIANT:BCFTOOLS_ANNOTATE' { + ext.args = "-c CHROM,FROM,TO,FOUND_IN --output-type z" + ext.prefix = { "${meta.id}_split_rmdup_info" } + } } diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index f39cb40c..cb40d008 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -43,6 +43,7 @@ params { known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" + score_config_mt = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" score_config_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" score_config_sv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_sv.ini" svdb_query_dbs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/svdb_querydb_files.csv" diff --git a/lib/WorkflowRaredisease.groovy b/lib/WorkflowRaredisease.groovy index f45fcc7c..b6b58af6 100755 --- a/lib/WorkflowRaredisease.groovy +++ b/lib/WorkflowRaredisease.groovy @@ -35,6 +35,20 @@ class WorkflowRaredisease { return outfile } + // + // Create a bed file which includes the name of variant caller from fai + // + public static String makeBedWithVariantCallerInfo(fai, parent_dir, varcaller) { + def outfile = new File(parent_dir + '/' + varcaller +'.bed') + def writer = outfile.newWriter() + fai.eachLine { line -> + def split_str = line.tokenize("\t") + writer << [split_str[0],"1",split_str[1],varcaller].join("\t") + "\n" + } + writer.close() + return outfile + } + // // Get workflow summary for MultiQC // diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index 6413f896..fdcc4629 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -28,6 +28,7 @@ workflow CALL_SNV { ch_call_interval // channel: [mandatory] [ path(intervals) ] ch_ml_model // channel: [mandatory] [ path(model) ] ch_case_info // channel: [mandatory] [ val(case_info) ] + ch_foundin_header // channel: [mandatory] [ path(header) ] ch_pcr_indel_model // channel: [optional] [ val(sentieon_dnascope_pcr_indel_model) ] main: @@ -42,7 +43,8 @@ workflow CALL_SNV { ch_genome_bam_bai, ch_genome_fasta, ch_genome_fai, - ch_case_info + ch_case_info, + ch_foundin_header ) ch_deepvar_vcf = CALL_SNV_DEEPVARIANT.out.vcf ch_deepvar_tbi = CALL_SNV_DEEPVARIANT.out.tabix diff --git a/subworkflows/local/variant_calling/call_snv_deepvariant.nf b/subworkflows/local/variant_calling/call_snv_deepvariant.nf index 8324aa35..197869b8 100644 --- a/subworkflows/local/variant_calling/call_snv_deepvariant.nf +++ b/subworkflows/local/variant_calling/call_snv_deepvariant.nf @@ -2,18 +2,22 @@ // A variant caller workflow for deepvariant // -include { BCFTOOLS_NORM as SPLIT_MULTIALLELICS_GL } from '../../../modules/nf-core/bcftools/norm/main' -include { BCFTOOLS_NORM as REMOVE_DUPLICATES_GL } from '../../../modules/nf-core/bcftools/norm/main' -include { DEEPVARIANT } from '../../../modules/nf-core/deepvariant/main' -include { GLNEXUS } from '../../../modules/nf-core/glnexus/main' -include { TABIX_TABIX as TABIX_GL } from '../../../modules/nf-core/tabix/tabix/main' +include { BCFTOOLS_ANNOTATE } from '../../../modules/nf-core/bcftools/annotate/main' +include { BCFTOOLS_NORM as SPLIT_MULTIALLELICS_GL } from '../../../modules/nf-core/bcftools/norm/main' +include { BCFTOOLS_NORM as REMOVE_DUPLICATES_GL } from '../../../modules/nf-core/bcftools/norm/main' +include { DEEPVARIANT } from '../../../modules/nf-core/deepvariant/main' +include { GLNEXUS } from '../../../modules/nf-core/glnexus/main' +include { TABIX_BGZIPTABIX as ZIP_TABIX_VARCALLERBED } from '../../../modules/nf-core/tabix/bgziptabix/main' +include { TABIX_TABIX as TABIX_GL } from '../../../modules/nf-core/tabix/tabix/main' +include { TABIX_TABIX as TABIX_ANNOTATE } from '../../../modules/nf-core/tabix/tabix/main' workflow CALL_SNV_DEEPVARIANT { take: - ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] - ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] - ch_case_info // channel: [mandatory] [ val(case_info) ] + ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_case_info // channel: [mandatory] [ val(case_info) ] + ch_foundin_header // channel: [mandatory] [ path(header) ] main: ch_versions = Channel.empty() @@ -48,6 +52,25 @@ workflow CALL_SNV_DEEPVARIANT { TABIX_GL (REMOVE_DUPLICATES_GL.out.vcf) + ch_genome_fai.map{meta, fai -> + return [meta, WorkflowRaredisease.makeBedWithVariantCallerInfo(fai, fai.parent.toString(), "deepvariant")] + } + .set { ch_varcallerinfo } + + ZIP_TABIX_VARCALLERBED (ch_varcallerinfo).gz_tbi + .map{meta,bed,tbi -> return [bed, tbi]} + .set{ch_varcallerbed} + + REMOVE_DUPLICATES_GL.out.vcf + .join(TABIX_GL.out.tbi) + .combine(ch_varcallerbed) + .combine(ch_foundin_header) + .set { ch_annotate_in } + + BCFTOOLS_ANNOTATE(ch_annotate_in) + + TABIX_ANNOTATE(BCFTOOLS_ANNOTATE.out.vcf) + ch_versions = ch_versions.mix(DEEPVARIANT.out.versions.first()) ch_versions = ch_versions.mix(GLNEXUS.out.versions) ch_versions = ch_versions.mix(SPLIT_MULTIALLELICS_GL.out.versions) @@ -55,7 +78,7 @@ workflow CALL_SNV_DEEPVARIANT { ch_versions = ch_versions.mix(TABIX_GL.out.versions) emit: - vcf = REMOVE_DUPLICATES_GL.out.vcf // channel: [ val(meta), path(vcf) ] - tabix = TABIX_GL.out.tbi // channel: [ val(meta), path(tbi) ] + vcf = BCFTOOLS_ANNOTATE.out.vcf // channel: [ val(meta), path(vcf) ] + tabix = TABIX_ANNOTATE.out.tbi // channel: [ val(meta), path(tbi) ] versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index c4183c93..203b3b5b 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -220,6 +220,7 @@ workflow RAREDISEASE { : Channel.value([[:],[]]) ch_dbsnp_tbi = params.known_dbsnp_tbi ? Channel.fromPath(params.known_dbsnp_tbi).map {it -> [[id:it[0].simpleName], it]}.collect() : ch_references.known_dbsnp_tbi.ifEmpty([[],[]]) + ch_foundin_header = Channel.fromPath("$projectDir/assets/foundin.hdr", checkIfExists: true).collect() ch_gcnvcaller_model = params.gcnvcaller_model ? Channel.fromPath(params.gcnvcaller_model).splitCsv ( header:true ) .map { row -> return [[id:file(row.models).simpleName], row.models] @@ -380,6 +381,7 @@ workflow RAREDISEASE { ch_call_interval, ch_ml_model, ch_case_info, + ch_foundin_header, Channel.value(params.sentieon_dnascope_pcr_indel_model) ) ch_versions = ch_versions.mix(CALL_SNV.out.versions) From 7157ec5f3289ecc727a52bd1b5b4f24ccbdcf7df Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 10 Jan 2024 07:49:00 +0100 Subject: [PATCH 219/387] found in sentieon and mutect2 --- conf/modules/call_snv_sentieon.config | 9 ++++ conf/modules/postprocess_MT_calls.config | 11 +++- subworkflows/local/call_snv.nf | 6 ++- .../variant_calling/call_snv_deepvariant.nf | 3 ++ .../variant_calling/call_snv_sentieon.nf | 52 ++++++++++++++----- .../variant_calling/postprocess_MT_calls.nf | 36 +++++++++++-- 6 files changed, 96 insertions(+), 21 deletions(-) diff --git a/conf/modules/call_snv_sentieon.config b/conf/modules/call_snv_sentieon.config index 3abcd31c..2337ef4f 100644 --- a/conf/modules/call_snv_sentieon.config +++ b/conf/modules/call_snv_sentieon.config @@ -48,5 +48,14 @@ process { ext.args = '--output-type z --rm-dup none' ext.prefix = { "${meta.id}_split_rmdup" } } + + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:ZIP_TABIX_VARCALLERBED' { + ext.args2 = '-s 1 -b 2 -e 3' + } + + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:BCFTOOLS_ANNOTATE' { + ext.args = "-c CHROM,FROM,TO,FOUND_IN --output-type z" + ext.prefix = { "${meta.id}_split_rmdup_info" } + } } } diff --git a/conf/modules/postprocess_MT_calls.config b/conf/modules/postprocess_MT_calls.config index c97ea43d..07e266de 100644 --- a/conf/modules/postprocess_MT_calls.config +++ b/conf/modules/postprocess_MT_calls.config @@ -37,6 +37,15 @@ process { withName: '.*POSTPROCESS_MT_CALLS:BCFTOOLS_MERGE_MT' { ext.args = '--output-type z' + ext.prefix = { "${meta.id}_split_rmdup_merged" } + } + + withName: '.*POSTPROCESS_MT_CALLS:ZIP_TABIX_VARCALLERBED' { + ext.args2 = '-s 1 -b 2 -e 3' + } + + withName: '.*POSTPROCESS_MT_CALLS:BCFTOOLS_ANNOTATE' { + ext.args = "-c CHROM,FROM,TO,FOUND_IN --output-type z" ext.prefix = { "${meta.id}_mitochondria" } publishDir = [ path: { "${params.outdir}/call_snv/mitochondria" }, @@ -45,7 +54,7 @@ process { ] } - withName: '.*POSTPROCESS_MT_CALLS:TABIX_TABIX_MERGE' { + withName: '.*POSTPROCESS_MT_CALLS:TABIX_ANNOTATE' { publishDir = [ path: { "${params.outdir}/call_snv/mitochondria" }, mode: params.publish_dir_mode, diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index fdcc4629..0f646461 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -59,7 +59,8 @@ workflow CALL_SNV { ch_call_interval, ch_ml_model, ch_case_info, - ch_pcr_indel_model + ch_pcr_indel_model, + ch_foundin_header ) ch_sentieon_vcf = CALL_SNV_SENTIEON.out.vcf ch_sentieon_tbi = CALL_SNV_SENTIEON.out.tabix @@ -102,7 +103,8 @@ workflow CALL_SNV { ch_genome_dictionary, ch_genome_fai, ch_mtshift_backchain, - ch_case_info + ch_case_info, + ch_foundin_header ) ch_versions = ch_versions.mix(CALL_SNV_MT.out.versions) diff --git a/subworkflows/local/variant_calling/call_snv_deepvariant.nf b/subworkflows/local/variant_calling/call_snv_deepvariant.nf index 197869b8..6ffea594 100644 --- a/subworkflows/local/variant_calling/call_snv_deepvariant.nf +++ b/subworkflows/local/variant_calling/call_snv_deepvariant.nf @@ -76,6 +76,9 @@ workflow CALL_SNV_DEEPVARIANT { ch_versions = ch_versions.mix(SPLIT_MULTIALLELICS_GL.out.versions) ch_versions = ch_versions.mix(REMOVE_DUPLICATES_GL.out.versions) ch_versions = ch_versions.mix(TABIX_GL.out.versions) + ch_versions = ch_versions.mix(ZIP_TABIX_VARCALLERBED.out.versions) + ch_versions = ch_versions.mix(BCFTOOLS_ANNOTATE.out.versions) + ch_versions = ch_versions.mix(TABIX_ANNOTATE.out.versions) emit: vcf = BCFTOOLS_ANNOTATE.out.vcf // channel: [ val(meta), path(vcf) ] diff --git a/subworkflows/local/variant_calling/call_snv_sentieon.nf b/subworkflows/local/variant_calling/call_snv_sentieon.nf index a9f5d4f8..bccc830a 100644 --- a/subworkflows/local/variant_calling/call_snv_sentieon.nf +++ b/subworkflows/local/variant_calling/call_snv_sentieon.nf @@ -2,15 +2,18 @@ // A subworkflow to call SNVs by sentieon dnascope with a machine learning model. // -include { SENTIEON_DNASCOPE } from '../../../modules/nf-core/sentieon/dnascope/main' -include { SENTIEON_DNAMODELAPPLY } from '../../../modules/nf-core/sentieon/dnamodelapply/main' -include { BCFTOOLS_MERGE } from '../../../modules/nf-core/bcftools/merge/main' -include { BCFTOOLS_NORM as SPLIT_MULTIALLELICS_SEN } from '../../../modules/nf-core/bcftools/norm/main' -include { BCFTOOLS_NORM as REMOVE_DUPLICATES_SEN } from '../../../modules/nf-core/bcftools/norm/main' -include { TABIX_TABIX as TABIX_SEN } from '../../../modules/nf-core/tabix/tabix/main' -include { TABIX_TABIX as TABIX_BCFTOOLS } from '../../../modules/nf-core/tabix/tabix/main' -include { BCFTOOLS_FILTER as BCF_FILTER_ONE } from '../../../modules/nf-core/bcftools/filter/main' -include { BCFTOOLS_FILTER as BCF_FILTER_TWO } from '../../../modules/nf-core/bcftools/filter/main' +include { SENTIEON_DNASCOPE } from '../../../modules/nf-core/sentieon/dnascope/main' +include { SENTIEON_DNAMODELAPPLY } from '../../../modules/nf-core/sentieon/dnamodelapply/main' +include { BCFTOOLS_MERGE } from '../../../modules/nf-core/bcftools/merge/main' +include { BCFTOOLS_NORM as SPLIT_MULTIALLELICS_SEN } from '../../../modules/nf-core/bcftools/norm/main' +include { BCFTOOLS_NORM as REMOVE_DUPLICATES_SEN } from '../../../modules/nf-core/bcftools/norm/main' +include { TABIX_TABIX as TABIX_SEN } from '../../../modules/nf-core/tabix/tabix/main' +include { TABIX_TABIX as TABIX_BCFTOOLS } from '../../../modules/nf-core/tabix/tabix/main' +include { BCFTOOLS_FILTER as BCF_FILTER_ONE } from '../../../modules/nf-core/bcftools/filter/main' +include { BCFTOOLS_FILTER as BCF_FILTER_TWO } from '../../../modules/nf-core/bcftools/filter/main' +include { BCFTOOLS_ANNOTATE } from '../../../modules/nf-core/bcftools/annotate/main' +include { TABIX_TABIX as TABIX_ANNOTATE } from '../../../modules/nf-core/tabix/tabix/main' +include { TABIX_BGZIPTABIX as ZIP_TABIX_VARCALLERBED } from '../../../modules/nf-core/tabix/bgziptabix/main' workflow CALL_SNV_SENTIEON { take: @@ -23,7 +26,8 @@ workflow CALL_SNV_SENTIEON { ch_ml_model // channel: [mandatory] [ val(meta), path(model) ] ch_case_info // channel: [mandatory] [ val(case_info) ] ch_pcr_indel_model // channel: [optional] [ val(sentieon_dnascope_pcr_indel_model) ] - + ch_foundin_header // channel: [mandatory] [ path(header) ] + main: ch_versions = Channel.empty() @@ -85,6 +89,25 @@ workflow CALL_SNV_SENTIEON { TABIX_SEN(REMOVE_DUPLICATES_SEN.out.vcf) + ch_genome_fai.map{meta, fai -> + return [meta, WorkflowRaredisease.makeBedWithVariantCallerInfo(fai, fai.parent.toString(), "sentieon")] + } + .set { ch_varcallerinfo } + + ZIP_TABIX_VARCALLERBED (ch_varcallerinfo).gz_tbi + .map{meta,bed,tbi -> return [bed, tbi]} + .set{ch_varcallerbed} + + REMOVE_DUPLICATES_SEN.out.vcf + .join(TABIX_SEN.out.tbi) + .combine(ch_varcallerbed) + .combine(ch_foundin_header) + .set { ch_annotate_in } + + BCFTOOLS_ANNOTATE(ch_annotate_in) + + TABIX_ANNOTATE(BCFTOOLS_ANNOTATE.out.vcf) + ch_versions = ch_versions.mix(SENTIEON_DNASCOPE.out.versions.first()) ch_versions = ch_versions.mix(SENTIEON_DNAMODELAPPLY.out.versions.first()) ch_versions = ch_versions.mix(BCFTOOLS_MERGE.out.versions.first()) @@ -92,9 +115,12 @@ workflow CALL_SNV_SENTIEON { ch_versions = ch_versions.mix(REMOVE_DUPLICATES_SEN.out.versions.first()) ch_versions = ch_versions.mix(TABIX_SEN.out.versions.first()) ch_versions = ch_versions.mix(BCF_FILTER_ONE.out.versions.first()) + ch_versions = ch_versions.mix(ZIP_TABIX_VARCALLERBED.out.versions) + ch_versions = ch_versions.mix(BCFTOOLS_ANNOTATE.out.versions) + ch_versions = ch_versions.mix(TABIX_ANNOTATE.out.versions) emit: - vcf = REMOVE_DUPLICATES_SEN.out.vcf // channel: [ val(meta), path(vcf) ] - tabix = TABIX_SEN.out.tbi // channel: [ val(meta), path(tbi) ] - versions = ch_versions // channel: [ path(versions.yml) ] + vcf = BCFTOOLS_ANNOTATE.out.vcf // channel: [ val(meta), path(vcf) ] + tabix = TABIX_ANNOTATE.out.tbi // channel: [ val(meta), path(tbi) ] + versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/subworkflows/local/variant_calling/postprocess_MT_calls.nf b/subworkflows/local/variant_calling/postprocess_MT_calls.nf index cb14c9b7..304d8bd4 100644 --- a/subworkflows/local/variant_calling/postprocess_MT_calls.nf +++ b/subworkflows/local/variant_calling/postprocess_MT_calls.nf @@ -11,6 +11,9 @@ include { TABIX_TABIX as TABIX_TABIX_MT2 } from '../../.. include { BCFTOOLS_MERGE as BCFTOOLS_MERGE_MT } from '../../../modules/nf-core/bcftools/merge/main' include { TABIX_TABIX as TABIX_TABIX_MERGE } from '../../../modules/nf-core/tabix/tabix/main' include { PICARD_LIFTOVERVCF } from '../../../modules/nf-core/picard/liftovervcf/main' +include { BCFTOOLS_ANNOTATE } from '../../../modules/nf-core/bcftools/annotate/main' +include { TABIX_BGZIPTABIX as ZIP_TABIX_VARCALLERBED } from '../../../modules/nf-core/tabix/bgziptabix/main' +include { TABIX_TABIX as TABIX_ANNOTATE } from '../../../modules/nf-core/tabix/tabix/main' workflow POSTPROCESS_MT_CALLS { take: @@ -21,6 +24,7 @@ workflow POSTPROCESS_MT_CALLS { ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] ch_mtshift_backchain // channel: [mandatory] [ val(meta), path(backchain) ] ch_case_info // channel: [mandatory] [ val(case_info) ] + ch_foundin_header // channel: [mandatory] [ path(header) ] main: ch_versions = Channel.empty() @@ -93,9 +97,28 @@ workflow POSTPROCESS_MT_CALLS { BCFTOOLS_MERGE_MT.out.merged_variants .mix(ch_case_vcf.single) - .set { ch_annotation_in } + .set { ch_addfoundintag_in } - TABIX_TABIX_MERGE(ch_annotation_in) + TABIX_TABIX_MERGE(ch_addfoundintag_in) + + ch_genome_fai.map{meta, fai -> + return [meta, WorkflowRaredisease.makeBedWithVariantCallerInfo(fai, fai.parent.toString(), "mutect2")] + } + .set { ch_varcallerinfo } + + ZIP_TABIX_VARCALLERBED (ch_varcallerinfo).gz_tbi + .map{meta,bed,tbi -> return [bed, tbi]} + .set{ch_varcallerbed} + + ch_addfoundintag_in + .join(TABIX_TABIX_MERGE.out.tbi) + .combine(ch_varcallerbed) + .combine(ch_foundin_header) + .set { ch_annotate_in } + + BCFTOOLS_ANNOTATE(ch_annotate_in) + + TABIX_ANNOTATE(BCFTOOLS_ANNOTATE.out.vcf) ch_versions = ch_versions.mix(PICARD_LIFTOVERVCF.out.versions.first()) ch_versions = ch_versions.mix(GATK4_MERGEVCFS_LIFT_UNLIFT_MT.out.versions.first()) @@ -103,9 +126,12 @@ workflow POSTPROCESS_MT_CALLS { ch_versions = ch_versions.mix(SPLIT_MULTIALLELICS_MT.out.versions.first()) ch_versions = ch_versions.mix(REMOVE_DUPLICATES_MT.out.versions.first()) ch_versions = ch_versions.mix(BCFTOOLS_MERGE_MT.out.versions) + ch_versions = ch_versions.mix(ZIP_TABIX_VARCALLERBED.out.versions) + ch_versions = ch_versions.mix(BCFTOOLS_ANNOTATE.out.versions) + ch_versions = ch_versions.mix(TABIX_ANNOTATE.out.versions) emit: - vcf = ch_annotation_in // channel: [ val(meta), path(vcf) ] - tbi = TABIX_TABIX_MERGE.out.tbi // channel: [ val(meta), path(tbi) ] - versions = ch_versions // channel: [ path(versions.yml) ] + vcf = BCFTOOLS_ANNOTATE.out.vcf // channel: [ val(meta), path(vcf) ] + tbi = TABIX_ANNOTATE.out.tbi // channel: [ val(meta), path(tbi) ] + versions = ch_versions // channel: [ path(versions.yml) ] } From 9f36e558939608b971b0eca1d42ae86a8d246ce3 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 10 Jan 2024 08:23:36 +0100 Subject: [PATCH 220/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index ce1a867b..303d8c07 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -19,6 +19,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - ngsbits samplegender to check sex [#453](https://github.com/nf-core/raredisease/pull/453) - New workflow for generating cgh files from SV vcfs for interpretation in the CytosSure interpretation software. Turned off by default [#456](https://github.com/nf-core/raredisease/pull/456/) - Fastp to do adapter trimming. It can be skipped using `--skip_fastp` [#457](https://github.com/nf-core/raredisease/pull/457) +- Add FOUND_IN tag, which mentions the variant caller that found the mutation, in the INFO column of the vcf files [#471](https://github.com/nf-core/raredisease/pull/471) ### `Changed` From 084d23228888e5927e9af1ab2e2519c931c93e62 Mon Sep 17 00:00:00 2001 From: Jakob Willforss Date: Wed, 10 Jan 2024 08:44:58 +0100 Subject: [PATCH 221/387] Cleanup --- .../local/variant_calling/call_sv_germlinecnvcaller.nf | 4 ---- 1 file changed, 4 deletions(-) diff --git a/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf b/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf index 4e27b14c..5e4347c3 100644 --- a/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf +++ b/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf @@ -49,14 +49,10 @@ workflow CALL_SV_GERMLINECNVCALLER { GATK4_POSTPROCESSGERMLINECNVCALLS ( ch_postproc_in ) - // GATK4_POSTPROCESSGERMLINECNVCALLS.out.segments.view() - TABIX_TABIX(GATK4_POSTPROCESSGERMLINECNVCALLS.out.segments) - TABIX_TABIX.out.tbi.view() GATK4_POSTPROCESSGERMLINECNVCALLS.out.segments .join(TABIX_TABIX.out.tbi, failOnMismatch:true) .set {ch_segments_in} - ch_segments_in.view() // Filter out reference only (0/0) segments BCFTOOLS_VIEW (ch_segments_in , [], [], [] ) From 5972088a0bcfb0aa3b9251d1b3ae099646ed7838 Mon Sep 17 00:00:00 2001 From: Jakob Willforss Date: Wed, 10 Jan 2024 08:59:55 +0100 Subject: [PATCH 222/387] Changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index ce1a867b..da62b9db 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -19,6 +19,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - ngsbits samplegender to check sex [#453](https://github.com/nf-core/raredisease/pull/453) - New workflow for generating cgh files from SV vcfs for interpretation in the CytosSure interpretation software. Turned off by default [#456](https://github.com/nf-core/raredisease/pull/456/) - Fastp to do adapter trimming. It can be skipped using `--skip_fastp` [#457](https://github.com/nf-core/raredisease/pull/457) +- GATK CNVCaller uses segments instead of intervals, filters out "reference" segments between the calls, and fixes a bug with how `ch_readcount_intervals` was handled ### `Changed` From 938591d547701a2b93d58d435e978f94a4200ae1 Mon Sep 17 00:00:00 2001 From: Jakob Willforss Date: Wed, 10 Jan 2024 09:08:09 +0100 Subject: [PATCH 223/387] Correct documented suffixes in output data --- .../local/variant_calling/call_sv_germlinecnvcaller.nf | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf b/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf index 5e4347c3..ef2cf71e 100644 --- a/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf +++ b/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf @@ -64,10 +64,10 @@ workflow CALL_SV_GERMLINECNVCALLER { ch_versions = ch_versions.mix(BCFTOOLS_VIEW.out.versions) emit: - genotyped_intervals_vcf = GATK4_POSTPROCESSGERMLINECNVCALLS.out.intervals // channel: [ val(meta), path(*.tar.gz) ] - genotyped_segments_vcf = GATK4_POSTPROCESSGERMLINECNVCALLS.out.segments // channel: [ val(meta), path(*.tar.gz) ] - genotyped_filtered_segments_vcf = BCFTOOLS_VIEW.out.vcf // channel: [ val(meta), path(*.tar.gz) ] - denoised_vcf = GATK4_POSTPROCESSGERMLINECNVCALLS.out.denoised // channel: [ val(meta), path(*.tar.gz) ] + genotyped_intervals_vcf = GATK4_POSTPROCESSGERMLINECNVCALLS.out.intervals // channel: [ val(meta), path(*.vcf.gz) ] + genotyped_segments_vcf = GATK4_POSTPROCESSGERMLINECNVCALLS.out.segments // channel: [ val(meta), path(*.vcf.gz) ] + genotyped_filtered_segments_vcf = BCFTOOLS_VIEW.out.vcf // channel: [ val(meta), path(*.vcf.gz) ] + denoised_vcf = GATK4_POSTPROCESSGERMLINECNVCALLS.out.denoised // channel: [ val(meta), path(*.vcf.gz) ] versions = ch_versions // channel: [ versions.yml ] } From 7d413889c8f47a52b6a7eab240ecfa4690599d61 Mon Sep 17 00:00:00 2001 From: jemten Date: Wed, 10 Jan 2024 13:48:54 +0100 Subject: [PATCH 224/387] removing reference to patch in modules.json --- modules.json | 6 ++---- 1 file changed, 2 insertions(+), 4 deletions(-) diff --git a/modules.json b/modules.json index ea3913c2..0f435238 100644 --- a/modules.json +++ b/modules.json @@ -450,8 +450,7 @@ "tiddit/cov": { "branch": "master", "git_sha": "ae07bd3d3e229ca82bd94531648736d44367a391", - "installed_by": ["modules"], - "patch": "modules/nf-core/tiddit/cov/tiddit-cov.diff" + "installed_by": ["modules"] }, "tiddit/sv": { "branch": "master", @@ -476,8 +475,7 @@ "vcf2cytosure": { "branch": "master", "git_sha": "8694793954175ddd3001ddfbc0e36782a674d8a7", - "installed_by": ["modules"], - "patch": "modules/nf-core/vcf2cytosure/vcf2cytosure.diff" + "installed_by": ["modules"] }, "vcfanno": { "branch": "master", From 9bfc4c74edd074bede0c755eb5a2fb051c6030be Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 10 Jan 2024 14:36:35 +0100 Subject: [PATCH 225/387] add bwa mem --- modules/nf-core/bwa/mem/environment.yml | 9 + modules/nf-core/bwa/mem/main.nf | 55 ++++++ modules/nf-core/bwa/mem/meta.yml | 58 ++++++ modules/nf-core/bwa/mem/tests/main.nf.test | 172 ++++++++++++++++++ .../nf-core/bwa/mem/tests/main.nf.test.snap | 126 +++++++++++++ modules/nf-core/bwa/mem/tests/tags.yml | 3 + 6 files changed, 423 insertions(+) create mode 100644 modules/nf-core/bwa/mem/environment.yml create mode 100644 modules/nf-core/bwa/mem/main.nf create mode 100644 modules/nf-core/bwa/mem/meta.yml create mode 100644 modules/nf-core/bwa/mem/tests/main.nf.test create mode 100644 modules/nf-core/bwa/mem/tests/main.nf.test.snap create mode 100644 modules/nf-core/bwa/mem/tests/tags.yml diff --git a/modules/nf-core/bwa/mem/environment.yml b/modules/nf-core/bwa/mem/environment.yml new file mode 100644 index 00000000..c5b2a9ce --- /dev/null +++ b/modules/nf-core/bwa/mem/environment.yml @@ -0,0 +1,9 @@ +name: bwa_mem +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bwa=0.7.17 + # renovate: datasource=conda depName=bioconda/samtools + - samtools=1.18 diff --git a/modules/nf-core/bwa/mem/main.nf b/modules/nf-core/bwa/mem/main.nf new file mode 100644 index 00000000..a3a82994 --- /dev/null +++ b/modules/nf-core/bwa/mem/main.nf @@ -0,0 +1,55 @@ +process BWA_MEM { + tag "$meta.id" + label 'process_high' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:9c0128851101dafef65cef649826d2dbe6bedd7e-0' : + 'biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:9c0128851101dafef65cef649826d2dbe6bedd7e-0' }" + + input: + tuple val(meta), path(reads) + tuple val(meta2), path(index) + val sort_bam + + output: + tuple val(meta), path("*.bam"), emit: bam + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def samtools_command = sort_bam ? 'sort' : 'view' + """ + INDEX=`find -L ./ -name "*.amb" | sed 's/\\.amb\$//'` + + bwa mem \\ + $args \\ + -t $task.cpus \\ + \$INDEX \\ + $reads \\ + | samtools $samtools_command $args2 --threads $task.cpus -o ${prefix}.bam - + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/bwa/mem/meta.yml b/modules/nf-core/bwa/mem/meta.yml new file mode 100644 index 00000000..440fb1f9 --- /dev/null +++ b/modules/nf-core/bwa/mem/meta.yml @@ -0,0 +1,58 @@ +name: bwa_mem +description: Performs fastq alignment to a fasta reference using BWA +keywords: + - mem + - bwa + - alignment + - map + - fastq + - bam + - sam +tools: + - bwa: + description: | + BWA is a software package for mapping DNA sequences against + a large reference genome, such as the human genome. + homepage: http://bio-bwa.sourceforge.net/ + documentation: http://www.htslib.org/doc/samtools.html + arxiv: arXiv:1303.3997 + licence: ["GPL-3.0-or-later"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - meta2: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - index: + type: file + description: BWA genome index files + pattern: "Directory containing BWA index *.{amb,ann,bwt,pac,sa}" + - sort_bam: + type: boolean + description: use samtools sort (true) or samtools view (false) + pattern: "true or false" +output: + - bam: + type: file + description: Output BAM file containing read alignments + pattern: "*.{bam}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@jeremy1805" +maintainers: + - "@drpatelh" + - "@jeremy1805" diff --git a/modules/nf-core/bwa/mem/tests/main.nf.test b/modules/nf-core/bwa/mem/tests/main.nf.test new file mode 100644 index 00000000..b199bb70 --- /dev/null +++ b/modules/nf-core/bwa/mem/tests/main.nf.test @@ -0,0 +1,172 @@ +nextflow_process { + + name "Test Process BWA_MEM" + tag "modules_nfcore" + tag "modules" + tag "bwa" + tag "bwa/mem" + script "../main.nf" + process "BWA_MEM" + + test("Single-End") { + + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Single-End Sort") { + + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Paired-End") { + + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Paired-End Sort") { + + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } +} diff --git a/modules/nf-core/bwa/mem/tests/main.nf.test.snap b/modules/nf-core/bwa/mem/tests/main.nf.test.snap new file mode 100644 index 00000000..bfb55fc7 --- /dev/null +++ b/modules/nf-core/bwa/mem/tests/main.nf.test.snap @@ -0,0 +1,126 @@ +{ + "Single-End": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,df203d7c7e8fef351408a909570c7952" + ] + ], + "1": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ], + "bam": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,df203d7c7e8fef351408a909570c7952" + ] + ], + "versions": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ] + } + ], + "timestamp": "2023-12-04T11:01:22.483594641" + }, + "Single-End Sort": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,8a52bd78fdcecb994c1f63897d5b431c" + ] + ], + "1": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ], + "bam": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,8a52bd78fdcecb994c1f63897d5b431c" + ] + ], + "versions": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ] + } + ], + "timestamp": "2023-12-04T11:01:30.180783483" + }, + "Paired-End": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,9815aef9ec763a60c53c3879be2d73ae" + ] + ], + "1": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ], + "bam": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,9815aef9ec763a60c53c3879be2d73ae" + ] + ], + "versions": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ] + } + ], + "timestamp": "2023-12-04T11:01:38.761983007" + }, + "Paired-End Sort": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,0f0cda73704c4f7ba08af482edcbbe88" + ] + ], + "1": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ], + "bam": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,0f0cda73704c4f7ba08af482edcbbe88" + ] + ], + "versions": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ] + } + ], + "timestamp": "2023-12-04T11:01:46.284587802" + } +} \ No newline at end of file diff --git a/modules/nf-core/bwa/mem/tests/tags.yml b/modules/nf-core/bwa/mem/tests/tags.yml new file mode 100644 index 00000000..82992d1f --- /dev/null +++ b/modules/nf-core/bwa/mem/tests/tags.yml @@ -0,0 +1,3 @@ +bwa/mem: + - modules/nf-core/bwa/index/** + - modules/nf-core/bwa/mem/** From 9c178a1a51cf5046a503fdb60f560f5371d8c79c Mon Sep 17 00:00:00 2001 From: Anders Jemt Date: Wed, 10 Jan 2024 16:51:24 +0100 Subject: [PATCH 226/387] bring back a few lines that was commented out --- subworkflows/local/call_structural_variants.nf | 11 +++++------ workflows/raredisease.nf | 1 - 2 files changed, 5 insertions(+), 7 deletions(-) diff --git a/subworkflows/local/call_structural_variants.nf b/subworkflows/local/call_structural_variants.nf index 0007e251..ad45dc29 100644 --- a/subworkflows/local/call_structural_variants.nf +++ b/subworkflows/local/call_structural_variants.nf @@ -53,11 +53,10 @@ workflow CALL_STRUCTURAL_VARIANTS { ch_versions = ch_versions.mix(CALL_SV_GERMLINECNVCALLER.out.versions) } - // Uncomment - //CALL_SV_CNVNATOR (ch_genome_bam_bai, ch_genome_fasta, ch_genome_fai, ch_case_info) - // .vcf - // .collect{it[1]} - // .set { cnvnator_vcf } + CALL_SV_CNVNATOR (ch_genome_bam_bai, ch_genome_fasta, ch_genome_fai, ch_case_info) + .vcf + .collect{it[1]} + .set { cnvnator_vcf } CALL_SV_MT (ch_mt_bam_bai, ch_genome_fasta) @@ -65,7 +64,7 @@ workflow CALL_STRUCTURAL_VARIANTS { if (params.skip_germlinecnvcaller) { tiddit_vcf .combine(manta_vcf) - // .combine(cnvnator_vcf) + .combine(cnvnator_vcf) .toList() .set { vcf_list } } else { diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index cb23fe7d..76e37e47 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -242,7 +242,6 @@ workflow RAREDISEASE { : Channel.empty() ch_intervals_y = params.intervals_y ? Channel.fromPath(params.intervals_y).collect() : Channel.empty() - //ch_me_references = params.mobile_element_references ? Channel.fromPath(params.mobile_element_references) ch_me_references = params.mobile_element_references ? Channel.fromSamplesheet("mobile_element_references") : Channel.empty() ch_ml_model = params.variant_caller.equals("sentieon") ? Channel.fromPath(params.ml_model).map {it -> [[id:it[0].simpleName], it]}.collect() From 30c8b8311dc1a96b80f18327952f85d2cc610e4d Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 11 Jan 2024 07:44:10 +0100 Subject: [PATCH 227/387] add bwa --- conf/modules/align_MT.config | 17 +++++++ ...wamem2.config => align_bwa_bwamem2.config} | 46 +++++++++++++++++-- conf/modules/convert_mt_bam_to_fastq.config | 1 + conf/modules/prepare_references.config | 6 ++- modules.json | 5 ++ nextflow.config | 2 +- nextflow_schema.json | 2 +- subworkflows/local/align.nf | 15 +++--- subworkflows/local/alignment/align_MT.nf | 9 +++- ...{align_bwamem2.nf => align_bwa_bwamem2.nf} | 21 ++++++--- subworkflows/local/prepare_references.nf | 6 ++- 11 files changed, 106 insertions(+), 24 deletions(-) rename conf/modules/{align_bwamem2.config => align_bwa_bwamem2.config} (51%) rename subworkflows/local/alignment/{align_bwamem2.nf => align_bwa_bwamem2.nf} (79%) diff --git a/conf/modules/align_MT.config b/conf/modules/align_MT.config index 23ab1c8c..79c4e8d3 100644 --- a/conf/modules/align_MT.config +++ b/conf/modules/align_MT.config @@ -25,6 +25,14 @@ process { } } + if (params.aligner.equals("bwa")) { + withName: '.*ALIGN_MT:BWA_MEM_MT' { + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.when = { !(params.analysis_type.equals("wes")) } + ext.prefix = { "${meta.id}.sorted" } + } + } + if (params.aligner.equals("sentieon")) { withName: '.*ALIGN_MT:SENTIEON_BWAMEM_MT' { ext.args = { "-M -K 10000000 -R ${meta.read_group}" } @@ -81,6 +89,15 @@ process { } } + + if (params.aligner.equals("bwa")) { + withName: '.*ALIGN_MT_SHIFT:BWA_MEM_MT' { + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.when = { !(params.analysis_type.equals("wes")) } + ext.prefix = { "${meta.id}.sorted" } + } + } + withName: '.*ALIGN_MT_SHIFT:GATK4_MERGEBAMALIGNMENT_MT' { ext.args = '--CREATE_INDEX true --MAX_GAPS -1 --SORT_ORDER queryname --INCLUDE_SECONDARY_ALIGNMENTS false --PAIRED_RUN false --VALIDATION_STRINGENCY LENIENT' ext.prefix = { "${meta.id}_merged_shifted" } diff --git a/conf/modules/align_bwamem2.config b/conf/modules/align_bwa_bwamem2.config similarity index 51% rename from conf/modules/align_bwamem2.config rename to conf/modules/align_bwa_bwamem2.config index ba92f738..2ef55d8d 100644 --- a/conf/modules/align_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2.config @@ -18,20 +18,21 @@ process { if (params.aligner.equals("bwamem2")) { - withName: '.*ALIGN:ALIGN_BWAMEM2:BWAMEM2_MEM' { + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWAMEM2_MEM' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.prefix = { "${meta.id}.sorted" } + ext.when = { params.aligner.equals("bwamem2")} } - withName: '.*ALIGN:ALIGN_BWAMEM2:SAMTOOLS_STATS' { + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_STATS' { ext.args = '-s --remove-overlaps' } - withName: '.*ALIGN:ALIGN_BWAMEM2:SAMTOOLS_MERGE' { + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_MERGE' { ext.prefix = { "${meta.id}_sorted_merged" } } - withName: '.*ALIGN:ALIGN_BWAMEM2:MARKDUPLICATES' { + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:MARKDUPLICATES' { ext.prefix = { "${meta.id}_sorted_md" } publishDir = [ enabled: !params.save_mapped_as_cram, @@ -41,7 +42,42 @@ process { ] } - withName: '.*ALIGN:ALIGN_BWAMEM2:SAMTOOLS_INDEX_MARKDUP' { + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_INDEX_MARKDUP' { + publishDir = [ + enabled: !params.save_mapped_as_cram, + path: { "${params.outdir}/alignment" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + } + if (params.aligner.equals("bwa")) { + + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.prefix = { "${meta.id}.sorted" } + ext.when = { params.aligner.equals("bwa")} + } + + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_STATS' { + ext.args = '-s --remove-overlaps' + } + + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_MERGE' { + ext.prefix = { "${meta.id}_sorted_merged" } + } + + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:MARKDUPLICATES' { + ext.prefix = { "${meta.id}_sorted_md" } + publishDir = [ + enabled: !params.save_mapped_as_cram, + path: { "${params.outdir}/alignment" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_INDEX_MARKDUP' { publishDir = [ enabled: !params.save_mapped_as_cram, path: { "${params.outdir}/alignment" }, diff --git a/conf/modules/convert_mt_bam_to_fastq.config b/conf/modules/convert_mt_bam_to_fastq.config index 0365c452..07fb8177 100644 --- a/conf/modules/convert_mt_bam_to_fastq.config +++ b/conf/modules/convert_mt_bam_to_fastq.config @@ -19,6 +19,7 @@ process { withName: '.*ALIGN:CONVERT_MT_BAM_TO_FASTQ:GATK4_PRINTREADS_MT' { beforeScript = {"mkdir ./tmp"} + ext.prefix = { "${meta.id}.printreads" } ext.args = [ "-L ${params.mito_name}", "--read-filter MateOnSameContigOrNoMappedMateReadFilter", diff --git a/conf/modules/prepare_references.config b/conf/modules/prepare_references.config index 101824ef..39a0a807 100644 --- a/conf/modules/prepare_references.config +++ b/conf/modules/prepare_references.config @@ -41,8 +41,12 @@ process { ext.when = { !(params.analysis_type == "wes") && params.aligner == "sentieon"} } + withName: '.*PREPARE_REFERENCES:BWA_INDEX_MT_SHIFT' { + ext.when = { !(params.analysis_type == "wes") && params.aligner == "bwa"} + } + withName: '.*PREPARE_REFERENCES:BWA_INDEX_GENOME' { - ext.when = {!params.bwa && !(params.aligner == "sentieon")} + ext.when = {!params.bwa && (!(params.aligner == "sentieon") || params.aligner == "bwa")} } withName: '.*PREPARE_REFERENCES:SAMTOOLS_FAIDX_GENOME' { diff --git a/modules.json b/modules.json index e0a92fa6..049d09e5 100644 --- a/modules.json +++ b/modules.json @@ -55,6 +55,11 @@ "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, + "bwa/mem": { + "branch": "master", + "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "installed_by": ["modules"] + }, "bwamem2/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", diff --git a/nextflow.config b/nextflow.config index e65b343d..d19410d5 100644 --- a/nextflow.config +++ b/nextflow.config @@ -317,7 +317,7 @@ includeConfig 'conf/modules/qc_bam.config' includeConfig 'conf/modules/rank_variants.config' includeConfig 'conf/modules/scatter_genome.config' includeConfig 'conf/modules/align_MT.config' -includeConfig 'conf/modules/align_bwamem2.config' +includeConfig 'conf/modules/align_bwa_bwamem2.config' includeConfig 'conf/modules/align_sentieon.config' includeConfig 'conf/modules/annotate_cadd.config' includeConfig 'conf/modules/call_snv_MT.config' diff --git a/nextflow_schema.json b/nextflow_schema.json index 07988ec3..0801c5c1 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -476,7 +476,7 @@ "default": "bwamem2", "description": "Specifies the alignment algorithm to use - available options are 'bwamem2' and 'sentieon'.", "fa_icon": "fas fa-align-center", - "enum": ["bwamem2", "sentieon"] + "enum": ["bwa", "bwamem2", "sentieon"] }, "min_trimmed_length": { "type": "integer", diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index e7240f2c..51bbf3fb 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -3,7 +3,7 @@ // include { FASTP } from '../../modules/nf-core/fastp/main' -include { ALIGN_BWAMEM2 } from './alignment/align_bwamem2' +include { ALIGN_BWA_BWAMEM2 } from './alignment/align_bwa_bwamem2' include { ALIGN_SENTIEON } from './alignment/align_sentieon' include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main' include { ALIGN_MT } from './alignment/align_MT' @@ -39,18 +39,19 @@ workflow ALIGN { ch_versions = ch_versions.mix(FASTP.out.versions) ch_fastp_json = FASTP.out.json } - - if (params.aligner.equals("bwamem2")) { - ALIGN_BWAMEM2 ( // Triggered when params.aligner is set as bwamem2 + + if (params.aligner.equals("bwamem2") || params.aligner.equals("bwa")) { + ALIGN_BWA_BWAMEM2 ( // Triggered when params.aligner is set as bwamem2 or bwa ch_reads, + ch_genome_bwaindex, ch_genome_bwamem2index, ch_genome_fasta, ch_genome_fai, val_platform ) - ch_bwamem2_bam = ALIGN_BWAMEM2.out.marked_bam - ch_bwamem2_bai = ALIGN_BWAMEM2.out.marked_bai - ch_versions = ch_versions.mix(ALIGN_BWAMEM2.out.versions) + ch_bwamem2_bam = ALIGN_BWA_BWAMEM2.out.marked_bam + ch_bwamem2_bai = ALIGN_BWA_BWAMEM2.out.marked_bai + ch_versions = ch_versions.mix(ALIGN_BWA_BWAMEM2.out.versions) } else if (params.aligner.equals("sentieon")) { ALIGN_SENTIEON ( // Triggered when params.aligner is set as sentieon ch_reads, diff --git a/subworkflows/local/alignment/align_MT.nf b/subworkflows/local/alignment/align_MT.nf index 29903c72..8f7930c7 100644 --- a/subworkflows/local/alignment/align_MT.nf +++ b/subworkflows/local/alignment/align_MT.nf @@ -2,6 +2,7 @@ // Align MT // +include { BWA_MEM as BWA_MEM_MT } from '../../../modules/nf-core/bwa/mem/main' include { SENTIEON_BWAMEM as SENTIEON_BWAMEM_MT } from '../../../modules/nf-core/sentieon/bwamem/main' include { BWAMEM2_MEM as BWAMEM2_MEM_MT } from '../../../modules/nf-core/bwamem2/mem/main' include { GATK4_MERGEBAMALIGNMENT as GATK4_MERGEBAMALIGNMENT_MT } from '../../../modules/nf-core/gatk4/mergebamalignment/main' @@ -22,6 +23,7 @@ workflow ALIGN_MT { main: ch_versions = Channel.empty() + ch_bwa_bam = Channel.empty() ch_bwamem2_bam = Channel.empty() ch_sentieon_bam = Channel.empty() @@ -33,10 +35,13 @@ workflow ALIGN_MT { SENTIEON_BWAMEM_MT ( ch_fastq, ch_bwaindex, ch_fasta, ch_fai ) ch_sentieon_bam = SENTIEON_BWAMEM_MT.out.bam_and_bai.map{ meta, bam, bai -> [meta, bam] } ch_versions = ch_versions.mix(SENTIEON_BWAMEM_MT.out.versions.first()) + } else if (params.aligner.equals("bwa")) { + BWA_MEM_MT ( ch_fastq, ch_bwaindex, true ) + ch_bwa_bam = BWA_MEM_MT.out.bam + ch_versions = ch_versions.mix(BWA_MEM_MT.out.versions.first()) } - Channel.empty() - .mix(ch_bwamem2_bam, ch_sentieon_bam) + .mix(ch_bwamem2_bam, ch_sentieon_bam, ch_bwa_bam) .join(ch_ubam, failOnMismatch:true, failOnDuplicate:true) .set {ch_bam_ubam} diff --git a/subworkflows/local/alignment/align_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf similarity index 79% rename from subworkflows/local/alignment/align_bwamem2.nf rename to subworkflows/local/alignment/align_bwa_bwamem2.nf index 75bd723e..10537898 100644 --- a/subworkflows/local/alignment/align_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -2,6 +2,7 @@ // Map to reference, fetch stats for each demultiplexed read pair, merge, mark duplicates, and index. // +include { BWA_MEM } from '../../../modules/nf-core/bwa/mem/main' include { BWAMEM2_MEM } from '../../../modules/nf-core/bwamem2/mem/main' include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_ALIGN } from '../../../modules/nf-core/samtools/index/main' include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_MARKDUP } from '../../../modules/nf-core/samtools/index/main' @@ -10,9 +11,10 @@ include { SAMTOOLS_MERGE } from '../../../modules/nf-c include { PICARD_MARKDUPLICATES as MARKDUPLICATES } from '../../../modules/nf-core/picard/markduplicates/main' -workflow ALIGN_BWAMEM2 { +workflow ALIGN_BWA_BWAMEM2 { take: ch_reads_input // channel: [mandatory] [ val(meta), path(reads_input) ] + ch_bwa_index // channel: [mandatory] [ val(meta), path(bwamem2_index) ] ch_bwamem2_index // channel: [mandatory] [ val(meta), path(bwamem2_index) ] ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] @@ -22,16 +24,24 @@ workflow ALIGN_BWAMEM2 { ch_versions = Channel.empty() // Map, sort, and index - BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) + if (params.aligner.equals("bwa")) { + BWA_MEM ( ch_reads_input, ch_bwa_index, true ) + ch_align = BWA_MEM.out.bam + ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) + } else { + BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) + ch_align = BWAMEM2_MEM.out.bam + ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) + } - SAMTOOLS_INDEX_ALIGN ( BWAMEM2_MEM.out.bam ) + SAMTOOLS_INDEX_ALIGN ( ch_align ) // Get stats for each demultiplexed read pair. - bam_sorted_indexed = BWAMEM2_MEM.out.bam.join(SAMTOOLS_INDEX_ALIGN.out.bai, failOnMismatch:true, failOnDuplicate:true) + bam_sorted_indexed = ch_align.join(SAMTOOLS_INDEX_ALIGN.out.bai, failOnMismatch:true, failOnDuplicate:true) SAMTOOLS_STATS ( bam_sorted_indexed, [[],[]] ) // Merge multiple lane samples and index - BWAMEM2_MEM.out.bam + ch_align .map{ meta, bam -> new_id = meta.sample new_meta = meta + [id:new_id, read_group:"\'@RG\\tID:" + new_id + "\\tPL:" + val_platform + "\\tSM:" + new_id + "\'"] @@ -52,7 +62,6 @@ workflow ALIGN_BWAMEM2 { MARKDUPLICATES ( prepared_bam , ch_genome_fasta, ch_genome_fai ) SAMTOOLS_INDEX_MARKDUP ( MARKDUPLICATES.out.bam ) - ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) ch_versions = ch_versions.mix(SAMTOOLS_INDEX_ALIGN.out.versions.first()) ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions.first()) ch_versions = ch_versions.mix(SAMTOOLS_MERGE.out.versions.first()) diff --git a/subworkflows/local/prepare_references.nf b/subworkflows/local/prepare_references.nf index 9752b3f3..0e56251c 100644 --- a/subworkflows/local/prepare_references.nf +++ b/subworkflows/local/prepare_references.nf @@ -3,6 +3,7 @@ // include { BWA_INDEX as BWA_INDEX_GENOME } from '../../modules/nf-core/bwa/index/main' +include { BWA_INDEX as BWA_INDEX_MT_SHIFT } from '../../modules/nf-core/bwa/index/main' include { BWAMEM2_INDEX as BWAMEM2_INDEX_GENOME } from '../../modules/nf-core/bwamem2/index/main' include { BWAMEM2_INDEX as BWAMEM2_INDEX_MT_SHIFT } from '../../modules/nf-core/bwamem2/index/main' include { CAT_CAT as CAT_CAT_BAIT } from '../../modules/nf-core/cat/cat/main' @@ -58,7 +59,9 @@ workflow PREPARE_REFERENCES { GATK_SD_MT_SHIFT(ch_mt_fasta_in) GATK_SHIFTFASTA(ch_mt_fasta_in, SAMTOOLS_FAIDX_MT_SHIFT.out.fai, GATK_SD_MT_SHIFT.out.dict) BWAMEM2_INDEX_MT_SHIFT(GATK_SHIFTFASTA.out.shift_fa) + BWA_INDEX_MT_SHIFT(GATK_SHIFTFASTA.out.shift_fa) SENTIEON_BWAINDEX_MT_SHIFT(GATK_SHIFTFASTA.out.shift_fa) + ch_bwa_mtshift = Channel.empty().mix(SENTIEON_BWAINDEX_MT_SHIFT.out.index, BWA_INDEX_MT_SHIFT.out.index).collect() GATK_SHIFTFASTA.out.intervals .multiMap{ meta, files -> shift_intervals: @@ -106,6 +109,7 @@ workflow PREPARE_REFERENCES { ch_versions = ch_versions.mix(GATK_SD_MT_SHIFT.out.versions) ch_versions = ch_versions.mix(GATK_SHIFTFASTA.out.versions) ch_versions = ch_versions.mix(BWAMEM2_INDEX_MT_SHIFT.out.versions) + ch_versions = ch_versions.mix(BWA_INDEX_MT_SHIFT.out.versions) ch_versions = ch_versions.mix(SENTIEON_BWAINDEX_MT_SHIFT.out.versions) ch_versions = ch_versions.mix(TABIX_GNOMAD_AF.out.versions) ch_versions = ch_versions.mix(TABIX_PT.out.versions) @@ -132,7 +136,7 @@ workflow PREPARE_REFERENCES { mtshift_fai = GATK_SHIFTFASTA.out.shift_fai.collect() // channel: [ val(meta), path(fai) ] mtshift_fasta = GATK_SHIFTFASTA.out.shift_fa.collect() // channel: [ val(meta), path(fai) ] mtshift_dict = GATK_SHIFTFASTA.out.dict.collect() // channel: [ path(dict) ] - mtshift_bwa_index = SENTIEON_BWAINDEX_MT_SHIFT.out.index.collect() // channel: [ val(meta), path(index) ] + mtshift_bwa_index = ch_bwa_mtshift // channel: [ val(meta), path(index) ] mtshift_bwamem2_index = BWAMEM2_INDEX_MT_SHIFT.out.index.collect() // channel: [ val(meta), path(index) ] gnomad_af_idx = TABIX_GNOMAD_AF.out.tbi.collect() // channel: [ val(meta), path(fasta) ] From 88a8e4634feed8d4b2090f4e24652e94e50afee5 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 11 Jan 2024 08:06:32 +0100 Subject: [PATCH 228/387] update config --- conf/modules/convert_mt_bam_to_fastq.config | 1 - 1 file changed, 1 deletion(-) diff --git a/conf/modules/convert_mt_bam_to_fastq.config b/conf/modules/convert_mt_bam_to_fastq.config index 07fb8177..0365c452 100644 --- a/conf/modules/convert_mt_bam_to_fastq.config +++ b/conf/modules/convert_mt_bam_to_fastq.config @@ -19,7 +19,6 @@ process { withName: '.*ALIGN:CONVERT_MT_BAM_TO_FASTQ:GATK4_PRINTREADS_MT' { beforeScript = {"mkdir ./tmp"} - ext.prefix = { "${meta.id}.printreads" } ext.args = [ "-L ${params.mito_name}", "--read-filter MateOnSameContigOrNoMappedMateReadFilter", From b0d1f1faff77028490ff145ff2027cd71faa4d36 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 11 Jan 2024 08:42:32 +0100 Subject: [PATCH 229/387] update docs --- CHANGELOG.md | 3 ++- README.md | 1 + docs/output.md | 9 +++++++-- docs/usage.md | 39 ++++++++++++++++++++------------------- 4 files changed, 30 insertions(+), 22 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index da62b9db..2e2e9792 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -19,7 +19,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - ngsbits samplegender to check sex [#453](https://github.com/nf-core/raredisease/pull/453) - New workflow for generating cgh files from SV vcfs for interpretation in the CytosSure interpretation software. Turned off by default [#456](https://github.com/nf-core/raredisease/pull/456/) - Fastp to do adapter trimming. It can be skipped using `--skip_fastp` [#457](https://github.com/nf-core/raredisease/pull/457) -- GATK CNVCaller uses segments instead of intervals, filters out "reference" segments between the calls, and fixes a bug with how `ch_readcount_intervals` was handled +- GATK CNVCaller uses segments instead of intervals, filters out "reference" segments between the calls, and fixes a bug with how `ch_readcount_intervals` was handled [#472](https://github.com/nf-core/raredisease/pull/472) +- bwa aligner [#474](https://github.com/nf-core/raredisease/pull/474) ### `Changed` diff --git a/README.md b/README.md index 3c36427a..2e37233f 100644 --- a/README.md +++ b/README.md @@ -48,6 +48,7 @@ On release, automated continuous integration tests run the pipeline on a full-si **2. Alignment:** - [Bwa-mem2](https://github.com/bwa-mem2/bwa-mem2) +- [BWA](https://github.com/lh3/bwa) - [Sentieon DNAseq](https://support.sentieon.com/manual/DNAseq_usage/dnaseq/) **3. Variant calling - SNV:** diff --git a/docs/output.md b/docs/output.md index 53460628..c80c7a79 100644 --- a/docs/output.md +++ b/docs/output.md @@ -16,6 +16,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Alignment](#alignment) - [Mapping](#mapping) - [Bwa-mem2](#bwa-mem2) + - [BWA](#bwa) - [Sentieon bwa mem](#sentieon-bwa-mem) - [Duplicate marking](#duplicate-marking) - [Picard's MarkDuplicates](#picards-markduplicates) @@ -74,15 +75,19 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d [Bwa-mem2](https://github.com/bwa-mem2/bwa-mem2) used to map the reads to a reference genome. The aligned reads are coordinate sorted with samtools sort. These files are treated as intermediates and are not placed in the output folder by default. +##### BWA + +[BWA](https://github.com/lh3/bwa) used to map the reads to a reference genome. The aligned reads are coordinate sorted with samtools sort. These files are treated as intermediates and are not placed in the output folder by default. It is not the default aligner, but it can be chosen by setting `--aligner` option to bwa. + ##### Sentieon bwa mem -[Sentieon's bwa mem](https://support.sentieon.com/manual/DNAseq_usage/dnaseq/#map-reads-to-reference) is the software accelerated version of the bwa-mem algorithm. It is used to efficiently perform the alignment using BWA. Aligned reads are then coordinate sorted using Sentieon's [sort](https://support.sentieon.com/manual/usages/general/#util-syntax) utility. These files are treated as intermediates and are not placed in the output folder by default. It is not the default aligner, but it can be chosen over bwamem2 by setting `--aligner` option to sentieon. +[Sentieon's bwa mem](https://support.sentieon.com/manual/DNAseq_usage/dnaseq/#map-reads-to-reference) is the software accelerated version of the bwa-mem algorithm. It is used to efficiently perform the alignment using BWA. Aligned reads are then coordinate sorted using Sentieon's [sort](https://support.sentieon.com/manual/usages/general/#util-syntax) utility. These files are treated as intermediates and are not placed in the output folder by default. It is not the default aligner, but it can be chosen by setting `--aligner` option to sentieon. #### Duplicate marking ##### Picard's MarkDuplicates -[Picard MarkDuplicates](https://broadinstitute.github.io/picard/command-line-overview.html#MarkDuplicates) is used for marking PCR duplicates that can occur during library amplification. This is essential as the presence of such duplicates results in false inflated coverages, which in turn can lead to overly-confident genotyping calls during variant calling. Only reads aligned by Bwa-mem2 are processed by this tool. By default, alignment files are published in bam format. If you would like to store cram files instead, set `--save_mapped_as_cram` to true. +[Picard MarkDuplicates](https://broadinstitute.github.io/picard/command-line-overview.html#MarkDuplicates) is used for marking PCR duplicates that can occur during library amplification. This is essential as the presence of such duplicates results in false inflated coverages, which in turn can lead to overly-confident genotyping calls during variant calling. Only reads aligned by Bwa-mem2 and bwa are processed by this tool. By default, alignment files are published in bam format. If you would like to store cram files instead, set `--save_mapped_as_cram` to true.
Output files from Alignment diff --git a/docs/usage.md b/docs/usage.md index b4b500ce..b84b0978 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -10,20 +10,20 @@ Table of contents: - [Run nf-core/raredisease with test data](#run-nf-coreraredisease-with-test-data) - [Updating the pipeline](#updating-the-pipeline) - [Run nf-core/raredisease with your data](#run-nf-coreraredisease-with-your-data) - - [Samplesheet](#samplesheet) - - [Reference files and parameters](#reference-files-and-parameters) - - [1. Alignment](#1-alignment) - - [2. QC stats from the alignment files](#2-qc-stats-from-the-alignment-files) - - [3. Repeat expansions](#3-repeat-expansions) - - [4. Variant calling - SNV](#4-variant-calling---snv) - - [5. Variant calling - Structural variants](#5-variant-calling---structural-variants) - - [6. Copy number variant calling](#6-copy-number-variant-calling) - - [7. SNV annotation \& Ranking](#7-snv-annotation--ranking) - - [8. SV annotation \& Ranking](#8-sv-annotation--ranking) - - [9. Mitochondrial annotation](#9-mitochondrial-annotation) - - [Run the pipeline](#run-the-pipeline) - - [Direct input in CLI](#direct-input-in-cli) - - [Import from a config file (recommended)](#import-from-a-config-file-recommended) + - [Samplesheet](#samplesheet) + - [Reference files and parameters](#reference-files-and-parameters) + - [1. Alignment](#1-alignment) + - [2. QC stats from the alignment files](#2-qc-stats-from-the-alignment-files) + - [3. Repeat expansions](#3-repeat-expansions) + - [4. Variant calling - SNV](#4-variant-calling---snv) + - [5. Variant calling - Structural variants](#5-variant-calling---structural-variants) + - [6. Copy number variant calling](#6-copy-number-variant-calling) + - [7. SNV annotation \& Ranking](#7-snv-annotation--ranking) + - [8. SV annotation \& Ranking](#8-sv-annotation--ranking) + - [9. Mitochondrial annotation](#9-mitochondrial-annotation) + - [Run the pipeline](#run-the-pipeline) + - [Direct input in CLI](#direct-input-in-cli) + - [Import from a config file (recommended)](#import-from-a-config-file-recommended) - [Best practices](#best-practices) - [Core Nextflow arguments](#core-nextflow-arguments) - [`-profile`](#-profile) @@ -129,11 +129,11 @@ Do not use `-c ` to specify parameters as this will result in errors. Cust The above pipeline run specified with a params file in yaml format: -Note that the pipeline is modular in architecture. It offers you the flexibility to choose between different tools. For example, you can align with either bwamem2 or Sentieon BWA mem and call SNVs with either DeepVariant or Sentieon DNAscope. You also have the option to turn off sections of the pipeline if you do not want to run the. For example, snv annotation can be turned off by adding `--skip_snv_annotation` flag in the command line, or by setting it to true in a parameter file. This flexibility means that in any given analysis run, a combination of tools included in the pipeline will not be executed. So the pipeline is written in a way that can account for these differences while working with reference parameters. If a tool is not going to be executed during the course of a run, parameters used only by that tool need not be provided. For example, for SNV calling if you use DeepVariant as your variant caller, you need not provide the parameter `--ml_model`, which is only used by Sentieon DNAscope. +Note that the pipeline is modular in architecture. It offers you the flexibility to choose between different tools. For example, you can align with bwamem2 or bwa or Sentieon BWA mem and call SNVs with either DeepVariant or Sentieon DNAscope. You also have the option to turn off sections of the pipeline if you do not want to run the. For example, snv annotation can be turned off by adding `--skip_snv_annotation` flag in the command line, or by setting it to true in a parameter file. This flexibility means that in any given analysis run, a combination of tools included in the pipeline will not be executed. So the pipeline is written in a way that can account for these differences while working with reference parameters. If a tool is not going to be executed during the course of a run, parameters used only by that tool need not be provided. For example, for SNV calling if you use DeepVariant as your variant caller, you need not provide the parameter `--ml_model`, which is only used by Sentieon DNAscope. nf-core/raredisease consists of several tools used for various purposes. For convenience, we have grouped those tools under the following categories: -1. Alignment (bwamem2/Sentieon BWA mem) +1. Alignment (bwamem2/bwa/Sentieon BWA mem) 2. QC stats from the alignment files 3. Repeat expansions (ExpansionsHunter & Stranger) 4. Variant calling - SNV (DeepVariant/Sentieon DNAscope) @@ -155,13 +155,14 @@ The mandatory and optional parameters for each category are tabulated below. | ------------------------------ | ------------------------------ | | aligner1 | fasta_fai3 | | fasta | bwamem23 | -| platform | known_dbsnp4 | -| mito_name/mt_fasta2 | known_dbsnp_tbi4 | +| platform | bwa3 | +| mito_name/mt_fasta2 | known_dbsnp4 | +| | known_dbsnp_tbi4 | | | min_trimmed_length5 | 1Default value is bwamem2, but if you have a valid license for Sentieon, you have the option to use Sentieon as well.
2f If mito_name is provided, mt_fasta can be generated by the pipeline.
-3fasta_fai and bwamem2, if not provided by the user, will be generated by the pipeline when necessary.
+3fasta_fai, bwa, and bwamem2, if not provided by the user, will be generated by the pipeline when necessary.
4Used only by Sentieon.
5Default value is 40. Used only by fastp.
From 0d24dbf2bef82a771c4fd061e0f28e879c47a7da Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 11 Jan 2024 08:47:06 +0100 Subject: [PATCH 230/387] fix lint --- docs/usage.md | 28 ++++++++++++++-------------- 1 file changed, 14 insertions(+), 14 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index b84b0978..65421c3c 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -10,20 +10,20 @@ Table of contents: - [Run nf-core/raredisease with test data](#run-nf-coreraredisease-with-test-data) - [Updating the pipeline](#updating-the-pipeline) - [Run nf-core/raredisease with your data](#run-nf-coreraredisease-with-your-data) - - [Samplesheet](#samplesheet) - - [Reference files and parameters](#reference-files-and-parameters) - - [1. Alignment](#1-alignment) - - [2. QC stats from the alignment files](#2-qc-stats-from-the-alignment-files) - - [3. Repeat expansions](#3-repeat-expansions) - - [4. Variant calling - SNV](#4-variant-calling---snv) - - [5. Variant calling - Structural variants](#5-variant-calling---structural-variants) - - [6. Copy number variant calling](#6-copy-number-variant-calling) - - [7. SNV annotation \& Ranking](#7-snv-annotation--ranking) - - [8. SV annotation \& Ranking](#8-sv-annotation--ranking) - - [9. Mitochondrial annotation](#9-mitochondrial-annotation) - - [Run the pipeline](#run-the-pipeline) - - [Direct input in CLI](#direct-input-in-cli) - - [Import from a config file (recommended)](#import-from-a-config-file-recommended) + - [Samplesheet](#samplesheet) + - [Reference files and parameters](#reference-files-and-parameters) + - [1. Alignment](#1-alignment) + - [2. QC stats from the alignment files](#2-qc-stats-from-the-alignment-files) + - [3. Repeat expansions](#3-repeat-expansions) + - [4. Variant calling - SNV](#4-variant-calling---snv) + - [5. Variant calling - Structural variants](#5-variant-calling---structural-variants) + - [6. Copy number variant calling](#6-copy-number-variant-calling) + - [7. SNV annotation \& Ranking](#7-snv-annotation--ranking) + - [8. SV annotation \& Ranking](#8-sv-annotation--ranking) + - [9. Mitochondrial annotation](#9-mitochondrial-annotation) + - [Run the pipeline](#run-the-pipeline) + - [Direct input in CLI](#direct-input-in-cli) + - [Import from a config file (recommended)](#import-from-a-config-file-recommended) - [Best practices](#best-practices) - [Core Nextflow arguments](#core-nextflow-arguments) - [`-profile`](#-profile) From 0809a4083e7f7e28ff2476a639f7bd0f73aa46ce Mon Sep 17 00:00:00 2001 From: Alexander Koc Date: Thu, 11 Jan 2024 09:33:57 +0100 Subject: [PATCH 231/387] Change hardcoded platform value to params.platform --- conf/modules/align_MT.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules/align_MT.config b/conf/modules/align_MT.config index 23ab1c8c..2dcacdef 100644 --- a/conf/modules/align_MT.config +++ b/conf/modules/align_MT.config @@ -42,7 +42,7 @@ process { ext.args = { [ "--VALIDATION_STRINGENCY LENIENT", "--RGLB lib", - "--RGPL ILLUMINA", + "--RGPL ${params.platform}", "--RGPU barcode", "--RGSM ${meta.id}" ].join(' ').trim() } From a8b62e9a4c251a31797926ebda3bafedc445df48 Mon Sep 17 00:00:00 2001 From: Alexander Koc Date: Thu, 11 Jan 2024 10:47:07 +0100 Subject: [PATCH 232/387] Update Changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index da62b9db..3f7e0d50 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -37,6 +37,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Replaced the parameter skip_mt_analysis which was used to turn on/off the mitochondrial workflow [#419](https://github.com/nf-core/raredisease/pull/419) - Adds a new parameter skip_mt_annotation which can be used to turn on/off annotation and ranking for mitochondrial SNVs [#419](https://github.com/nf-core/raredisease/pull/419) - Changed the name of the parameter from `skip_cnv_calling` to `skip_germlinecnvcaller` [#435](https://github.com/nf-core/raredisease/pull/435) +- Change hardcoded platform value to params.platform in align_MT.config [#475](https://github.com/nf-core/raredisease/pull/475) ### `Fixed` From 829282e3057ff711521b19dd8d30de319921bd51 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 11 Jan 2024 12:45:16 +0100 Subject: [PATCH 233/387] review suggestions and switch fai to chromsizes --- lib/WorkflowRaredisease.groovy | 7 ++++--- subworkflows/local/call_snv.nf | 12 ++++++++---- .../local/variant_calling/call_snv_deepvariant.nf | 15 ++++++++------- .../local/variant_calling/call_snv_sentieon.nf | 5 +++-- .../local/variant_calling/postprocess_MT_calls.nf | 5 +++-- workflows/raredisease.nf | 1 + 6 files changed, 27 insertions(+), 18 deletions(-) diff --git a/lib/WorkflowRaredisease.groovy b/lib/WorkflowRaredisease.groovy index b6b58af6..02e91acd 100755 --- a/lib/WorkflowRaredisease.groovy +++ b/lib/WorkflowRaredisease.groovy @@ -36,12 +36,13 @@ class WorkflowRaredisease { } // - // Create a bed file which includes the name of variant caller from fai + // Create a bed file which includes the name of variant caller from chromsizes file // - public static String makeBedWithVariantCallerInfo(fai, parent_dir, varcaller) { + public static String makeBedWithVariantCallerInfo(chromsizes, varcaller) { + def parent_dir = chromsizes.parent.toString() def outfile = new File(parent_dir + '/' + varcaller +'.bed') def writer = outfile.newWriter() - fai.eachLine { line -> + chromsizes.eachLine { line -> def split_str = line.tokenize("\t") writer << [split_str[0],"1",split_str[1],varcaller].join("\t") + "\n" } diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index 0f646461..8d926f0e 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -13,7 +13,8 @@ workflow CALL_SNV { take: ch_genome_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] ch_mt_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] - ch_mtshift_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_mtshift_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_genome_chrsizes // channel: [mandatory] [ path(sizes) ] ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] @@ -44,7 +45,8 @@ workflow CALL_SNV { ch_genome_fasta, ch_genome_fai, ch_case_info, - ch_foundin_header + ch_foundin_header, + ch_genome_chrsizes ) ch_deepvar_vcf = CALL_SNV_DEEPVARIANT.out.vcf ch_deepvar_tbi = CALL_SNV_DEEPVARIANT.out.tabix @@ -60,7 +62,8 @@ workflow CALL_SNV { ch_ml_model, ch_case_info, ch_pcr_indel_model, - ch_foundin_header + ch_foundin_header, + ch_genome_chrsizes ) ch_sentieon_vcf = CALL_SNV_SENTIEON.out.vcf ch_sentieon_tbi = CALL_SNV_SENTIEON.out.tabix @@ -104,7 +107,8 @@ workflow CALL_SNV { ch_genome_fai, ch_mtshift_backchain, ch_case_info, - ch_foundin_header + ch_foundin_header, + ch_genome_chrsizes ) ch_versions = ch_versions.mix(CALL_SNV_MT.out.versions) diff --git a/subworkflows/local/variant_calling/call_snv_deepvariant.nf b/subworkflows/local/variant_calling/call_snv_deepvariant.nf index 6ffea594..b20e19ae 100644 --- a/subworkflows/local/variant_calling/call_snv_deepvariant.nf +++ b/subworkflows/local/variant_calling/call_snv_deepvariant.nf @@ -13,11 +13,12 @@ include { TABIX_TABIX as TABIX_ANNOTATE } from '../../../modules/nf workflow CALL_SNV_DEEPVARIANT { take: - ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] - ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] - ch_case_info // channel: [mandatory] [ val(case_info) ] - ch_foundin_header // channel: [mandatory] [ path(header) ] + ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_case_info // channel: [mandatory] [ val(case_info) ] + ch_foundin_header // channel: [mandatory] [ path(header) ] + ch_genome_chrsizes // channel: [mandatory] [ path(chrsizes) ] main: ch_versions = Channel.empty() @@ -52,8 +53,8 @@ workflow CALL_SNV_DEEPVARIANT { TABIX_GL (REMOVE_DUPLICATES_GL.out.vcf) - ch_genome_fai.map{meta, fai -> - return [meta, WorkflowRaredisease.makeBedWithVariantCallerInfo(fai, fai.parent.toString(), "deepvariant")] + ch_genome_chrsizes.flatten().map{chromsizes -> + return [[id:'deepvariant'], WorkflowRaredisease.makeBedWithVariantCallerInfo(chromsizes, "deepvariant")] } .set { ch_varcallerinfo } diff --git a/subworkflows/local/variant_calling/call_snv_sentieon.nf b/subworkflows/local/variant_calling/call_snv_sentieon.nf index bccc830a..53db6095 100644 --- a/subworkflows/local/variant_calling/call_snv_sentieon.nf +++ b/subworkflows/local/variant_calling/call_snv_sentieon.nf @@ -27,6 +27,7 @@ workflow CALL_SNV_SENTIEON { ch_case_info // channel: [mandatory] [ val(case_info) ] ch_pcr_indel_model // channel: [optional] [ val(sentieon_dnascope_pcr_indel_model) ] ch_foundin_header // channel: [mandatory] [ path(header) ] + ch_genome_chrsizes // channel: [mandatory] [ path(chrsizes) ] main: ch_versions = Channel.empty() @@ -89,8 +90,8 @@ workflow CALL_SNV_SENTIEON { TABIX_SEN(REMOVE_DUPLICATES_SEN.out.vcf) - ch_genome_fai.map{meta, fai -> - return [meta, WorkflowRaredisease.makeBedWithVariantCallerInfo(fai, fai.parent.toString(), "sentieon")] + ch_genome_chrsizes.flatten().map{chromsizes -> + return [[id:'sentieon_dnascope'], WorkflowRaredisease.makeBedWithVariantCallerInfo(chromsizes, "sentieon_dnascope")] } .set { ch_varcallerinfo } diff --git a/subworkflows/local/variant_calling/postprocess_MT_calls.nf b/subworkflows/local/variant_calling/postprocess_MT_calls.nf index 304d8bd4..81279a9c 100644 --- a/subworkflows/local/variant_calling/postprocess_MT_calls.nf +++ b/subworkflows/local/variant_calling/postprocess_MT_calls.nf @@ -25,6 +25,7 @@ workflow POSTPROCESS_MT_CALLS { ch_mtshift_backchain // channel: [mandatory] [ val(meta), path(backchain) ] ch_case_info // channel: [mandatory] [ val(case_info) ] ch_foundin_header // channel: [mandatory] [ path(header) ] + ch_genome_chrsizes // channel: [mandatory] [ path(chrsizes) ] main: ch_versions = Channel.empty() @@ -101,8 +102,8 @@ workflow POSTPROCESS_MT_CALLS { TABIX_TABIX_MERGE(ch_addfoundintag_in) - ch_genome_fai.map{meta, fai -> - return [meta, WorkflowRaredisease.makeBedWithVariantCallerInfo(fai, fai.parent.toString(), "mutect2")] + ch_genome_chrsizes.flatten().map{chromsizes -> + return [[id:'mutect2'], WorkflowRaredisease.makeBedWithVariantCallerInfo(chromsizes, "mutect2")] } .set { ch_varcallerinfo } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 203b3b5b..ec1a2609 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -367,6 +367,7 @@ workflow RAREDISEASE { ch_mapped.genome_bam_bai, ch_mapped.mt_bam_bai, ch_mapped.mtshift_bam_bai, + ch_genome_chrsizes, ch_genome_fasta, ch_genome_fai, ch_genome_dictionary, From a64bd131bfc042a33d337ede93a968147357e520 Mon Sep 17 00:00:00 2001 From: Anders Jemt Date: Thu, 11 Jan 2024 13:37:27 +0100 Subject: [PATCH 234/387] Update lib/WorkflowRaredisease.groovy --- lib/WorkflowRaredisease.groovy | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/lib/WorkflowRaredisease.groovy b/lib/WorkflowRaredisease.groovy index 02e91acd..578dc6d6 100755 --- a/lib/WorkflowRaredisease.groovy +++ b/lib/WorkflowRaredisease.groovy @@ -44,7 +44,7 @@ class WorkflowRaredisease { def writer = outfile.newWriter() chromsizes.eachLine { line -> def split_str = line.tokenize("\t") - writer << [split_str[0],"1",split_str[1],varcaller].join("\t") + "\n" + writer << [split_str[0],"0",split_str[1],varcaller].join("\t") + "\n" } writer.close() return outfile From 9564c642706b9260fa86ee5f8fbae963ee0e130d Mon Sep 17 00:00:00 2001 From: Anders Jemt Date: Thu, 11 Jan 2024 14:03:46 +0100 Subject: [PATCH 235/387] add meta2 and meta3 to retroseq_call meta.yml --- modules/local/retroseq/call/meta.yml | 10 ++++++++++ 1 file changed, 10 insertions(+) diff --git a/modules/local/retroseq/call/meta.yml b/modules/local/retroseq/call/meta.yml index 98aa0c04..4c1e5b6c 100644 --- a/modules/local/retroseq/call/meta.yml +++ b/modules/local/retroseq/call/meta.yml @@ -31,10 +31,20 @@ input: type: file description: Index of the sorted BAM file pattern: "*.bam" + - meta2: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` - fasta: type: file description: Reference genome in fasta format pattern: "*.fasta" + - meta3: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` - fai: type: file description: Reference FASTA index From 9bc4827c11fddf91386a7d55e094c9357757329b Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 11 Jan 2024 14:23:46 +0100 Subject: [PATCH 236/387] adds schema for svdb query inputs --- CHANGELOG.md | 3 ++- assets/svdb_query_bedpe_schema.json | 40 +++++++++++++++++++++++++++++ assets/svdb_query_vcf_schema.json | 40 +++++++++++++++++++++++++++++ nextflow_schema.json | 10 ++++++-- 4 files changed, 90 insertions(+), 3 deletions(-) create mode 100644 assets/svdb_query_bedpe_schema.json create mode 100644 assets/svdb_query_vcf_schema.json diff --git a/CHANGELOG.md b/CHANGELOG.md index da62b9db..a19a0959 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -19,7 +19,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - ngsbits samplegender to check sex [#453](https://github.com/nf-core/raredisease/pull/453) - New workflow for generating cgh files from SV vcfs for interpretation in the CytosSure interpretation software. Turned off by default [#456](https://github.com/nf-core/raredisease/pull/456/) - Fastp to do adapter trimming. It can be skipped using `--skip_fastp` [#457](https://github.com/nf-core/raredisease/pull/457) -- GATK CNVCaller uses segments instead of intervals, filters out "reference" segments between the calls, and fixes a bug with how `ch_readcount_intervals` was handled +- GATK CNVCaller uses segments instead of intervals, filters out "reference" segments between the calls, and fixes a bug with how `ch_readcount_intervals` was handled [#472](https://github.com/nf-core/raredisease/pull/472) ### `Changed` @@ -37,6 +37,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Replaced the parameter skip_mt_analysis which was used to turn on/off the mitochondrial workflow [#419](https://github.com/nf-core/raredisease/pull/419) - Adds a new parameter skip_mt_annotation which can be used to turn on/off annotation and ranking for mitochondrial SNVs [#419](https://github.com/nf-core/raredisease/pull/419) - Changed the name of the parameter from `skip_cnv_calling` to `skip_germlinecnvcaller` [#435](https://github.com/nf-core/raredisease/pull/435) +- Check SVDB query input files for existence and correct format [#476](https://github.com/nf-core/raredisease/pull/476) ### `Fixed` diff --git a/assets/svdb_query_bedpe_schema.json b/assets/svdb_query_bedpe_schema.json new file mode 100644 index 00000000..2d4331a1 --- /dev/null +++ b/assets/svdb_query_bedpe_schema.json @@ -0,0 +1,40 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/raredisease/master/assets/mobile_element_references_schema.json", + "title": "Schema for SVDB query - BEDPE", + "description": "Schema for the SVDB query database input, bedpe version", + "type": "array", + "items": { + "type": "object", + "properties": { + "filename": { + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.bedpe$", + "errorMessage": "Path to query database cannot contain spaces and must be a bedpe file" + }, + "in_freq_info_key": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "In frequency key cannot contain spaces" + }, + "in_allele_count_info_key": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "In allele count key cannot contain spaces" + }, + "out_freq_info_key": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Out frequency key must be provided and cannot contain spaces" + }, + "out_allele_count_info_key": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Out allele count key must be provided and cannot contain spaces" + } + }, + "required": ["filename", "out_freq_info_key", "out_allele_count_info_key"] + } +} diff --git a/assets/svdb_query_vcf_schema.json b/assets/svdb_query_vcf_schema.json new file mode 100644 index 00000000..0bb8540a --- /dev/null +++ b/assets/svdb_query_vcf_schema.json @@ -0,0 +1,40 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/raredisease/master/assets/mobile_element_references_schema.json", + "title": "Schema for SVDB query - VCF", + "description": "Schema for the SVDB query database input, VCF version", + "type": "array", + "items": { + "type": "object", + "properties": { + "filename": { + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.vcf?(\\.gz)?$", + "errorMessage": "Path to query database cannot contain spaces and must be a vcf file" + }, + "in_freq_info_key": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "In frequency key cannot contain spaces" + }, + "in_allele_count_info_key": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "In allele count key cannot contain spaces" + }, + "out_freq_info_key": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Out frequency key must be provided and cannot contain spaces" + }, + "out_allele_count_info_key": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Out allele count key must be provided and cannot contain spaces" + } + }, + "required": ["filename", "out_freq_info_key", "out_allele_count_info_key"] + } +} diff --git a/nextflow_schema.json b/nextflow_schema.json index 07988ec3..99fd3253 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -294,7 +294,10 @@ "format": "file-path", "description": "Databases used for structural variant annotation in chrA-posA-chrB-posB-type-count-frequency format.", "fa_icon": "fas fa-file-csv", - "help_text": "Path to comma-separated file containing information about the databases used for structural variant annotation." + "help_text": "Path to comma-separated file containing information about the databases used for structural variant annotation.", + "pattern": "^\\S+\\.csv$", + "mimetype": "text/csv", + "schema": "assets/svdb_query_bedpe_schema.json" }, "svdb_query_dbs": { "type": "string", @@ -302,7 +305,10 @@ "format": "file-path", "description": "Databases used for structural variant annotation in vcf format.", "fa_icon": "fas fa-file-csv", - "help_text": "Path to comma-separated file containing information about the databases used for structural variant annotation." + "help_text": "Path to comma-separated file containing information about the databases used for structural variant annotation.", + "pattern": "^\\S+\\.csv$", + "mimetype": "text/csv", + "schema": "assets/svdb_query_vcf_schema.json" }, "target_bed": { "type": "string", From 01a83137ff61268e64b85138f444361f5e9f2fc3 Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 11 Jan 2024 15:35:02 +0100 Subject: [PATCH 237/387] Document order of chr in refrence genome --- docs/usage.md | 19 ++++++++++--------- 1 file changed, 10 insertions(+), 9 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index b4b500ce..8dfd0cc4 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -153,17 +153,18 @@ The mandatory and optional parameters for each category are tabulated below. | Mandatory | Optional | | ------------------------------ | ------------------------------ | -| aligner1 | fasta_fai3 | -| fasta | bwamem23 | -| platform | known_dbsnp4 | -| mito_name/mt_fasta2 | known_dbsnp_tbi4 | -| | min_trimmed_length5 | +| aligner1 | fasta_fai4 | +| fasta2 | bwamem24 | +| platform | known_dbsnp5 | +| mito_name/mt_fasta3 | known_dbsnp_tbi5 | +| | min_trimmed_length6 | 1Default value is bwamem2, but if you have a valid license for Sentieon, you have the option to use Sentieon as well.
-2f If mito_name is provided, mt_fasta can be generated by the pipeline.
-3fasta_fai and bwamem2, if not provided by the user, will be generated by the pipeline when necessary.
-4Used only by Sentieon.
-5Default value is 40. Used only by fastp.
+2Analysis set reference genome in fasta format, first 25 contigs need to be chromosome 1-22, X, Y and the mitochondria.
+3f If mito_name is provided, mt_fasta can be generated by the pipeline.
+4fasta_fai and bwamem2, if not provided by the user, will be generated by the pipeline when necessary.
+5Used only by Sentieon.
+6Default value is 40. Used only by fastp.
##### 2. QC stats from the alignment files From 97be745b715ded5625b1f8204e2924a27f3a56f6 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 11 Jan 2024 17:52:09 +0100 Subject: [PATCH 238/387] review suggestion --- conf/modules/align_bwa_bwamem2.config | 32 ++------------------------- 1 file changed, 2 insertions(+), 30 deletions(-) diff --git a/conf/modules/align_bwa_bwamem2.config b/conf/modules/align_bwa_bwamem2.config index 2ef55d8d..4d8b59e3 100644 --- a/conf/modules/align_bwa_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2.config @@ -16,7 +16,8 @@ // process { - if (params.aligner.equals("bwamem2")) { + + if (params.aligner.equals("bwamem2") || params.aligner.equals("bwa")) { withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWAMEM2_MEM' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } @@ -24,35 +25,6 @@ process { ext.when = { params.aligner.equals("bwamem2")} } - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_STATS' { - ext.args = '-s --remove-overlaps' - } - - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_MERGE' { - ext.prefix = { "${meta.id}_sorted_merged" } - } - - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:MARKDUPLICATES' { - ext.prefix = { "${meta.id}_sorted_md" } - publishDir = [ - enabled: !params.save_mapped_as_cram, - path: { "${params.outdir}/alignment" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_INDEX_MARKDUP' { - publishDir = [ - enabled: !params.save_mapped_as_cram, - path: { "${params.outdir}/alignment" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - } - if (params.aligner.equals("bwa")) { - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.prefix = { "${meta.id}.sorted" } From e1963fb4fec61c34de251f73415d8621461cef7a Mon Sep 17 00:00:00 2001 From: Anders Jemt Date: Fri, 12 Jan 2024 17:32:31 +0100 Subject: [PATCH 239/387] Fix for getting chr sizes when fai is given --- subworkflows/local/prepare_references.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/prepare_references.nf b/subworkflows/local/prepare_references.nf index 9752b3f3..e91494c1 100644 --- a/subworkflows/local/prepare_references.nf +++ b/subworkflows/local/prepare_references.nf @@ -48,10 +48,10 @@ workflow PREPARE_REFERENCES { SENTIEON_BWAINDEX_GENOME(ch_genome_fasta).index.set{ch_sentieonbwa} SAMTOOLS_FAIDX_GENOME(ch_genome_fasta, [[],[]]) GATK_SD(ch_genome_fasta) - GET_CHROM_SIZES( SAMTOOLS_FAIDX_GENOME.out.fai ) + ch_fai = Channel.empty().mix(ch_genome_fai, SAMTOOLS_FAIDX_GENOME.out.fai).collect() + GET_CHROM_SIZES( ch_fai ) // MT indices - ch_fai = Channel.empty().mix(ch_genome_fai, SAMTOOLS_FAIDX_GENOME.out.fai).collect() SAMTOOLS_EXTRACT_MT(ch_genome_fasta, ch_fai) ch_mt_fasta_in = Channel.empty().mix(ch_mt_fasta, SAMTOOLS_EXTRACT_MT.out.fa).collect() SAMTOOLS_FAIDX_MT_SHIFT(ch_mt_fasta_in, [[],[]]) From 425863d72bc9879137e72206914faccdb7a334a9 Mon Sep 17 00:00:00 2001 From: Anders Jemt Date: Mon, 15 Jan 2024 09:19:03 +0100 Subject: [PATCH 240/387] updating usage --- docs/usage.md | 9 +++++---- 1 file changed, 5 insertions(+), 4 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 8dfd0cc4..80130c19 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -155,14 +155,15 @@ The mandatory and optional parameters for each category are tabulated below. | ------------------------------ | ------------------------------ | | aligner1 | fasta_fai4 | | fasta2 | bwamem24 | -| platform | known_dbsnp5 | -| mito_name/mt_fasta3 | known_dbsnp_tbi5 | +| platform | bwa4 | +| mito_name/mt_fasta3 | known_dbsnp5 | +| | known_dbsnp_tbi5 | | | min_trimmed_length6 | -1Default value is bwamem2, but if you have a valid license for Sentieon, you have the option to use Sentieon as well.
+1Default value is bwamem2. Other alternatives are bwa and sentieon (requires valid Sentieon license ).
2Analysis set reference genome in fasta format, first 25 contigs need to be chromosome 1-22, X, Y and the mitochondria.
3f If mito_name is provided, mt_fasta can be generated by the pipeline.
-4fasta_fai and bwamem2, if not provided by the user, will be generated by the pipeline when necessary.
+4fasta_fai, bwa and bwamem2, if not provided by the user, will be generated by the pipeline when necessary.
5Used only by Sentieon.
6Default value is 40. Used only by fastp.
From 1aed730b7c244cc4c19807b92fd1233335c10c4f Mon Sep 17 00:00:00 2001 From: Anders Jemt Date: Tue, 16 Jan 2024 13:38:27 +0100 Subject: [PATCH 241/387] fix number of me channels --- subworkflows/local/call_mobile_elements.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/subworkflows/local/call_mobile_elements.nf b/subworkflows/local/call_mobile_elements.nf index c77a11d9..fd26e6d5 100644 --- a/subworkflows/local/call_mobile_elements.nf +++ b/subworkflows/local/call_mobile_elements.nf @@ -83,15 +83,15 @@ workflow CALL_MOBILE_ELEMENTS { // Concatenate the chromosome vcfs to sample vcfs BCFTOOLS_SORT_ME.out.vcf .map { meta, vcf -> [ meta - meta.subMap('interval'), vcf ] } - .groupTuple(size: 24) + .groupTuple(size: 25) .set { ch_vcfs } TABIX_ME_SPLIT.out.tbi .map { meta, tbi -> [ meta - meta.subMap('interval'), tbi ] } - .groupTuple(size: 24) + .groupTuple(size: 25) .set { ch_tbis } - ch_vcfs.join(ch_tbis) + ch_vcfs.join(ch_tbis, failOnMismatch: true) .set { ch_vcfs_tbis} BCFTOOLS_CONCAT_ME ( ch_vcfs_tbis ) From 088a94ded0c78f955c8bb4c79f5d71f1d07c2bcd Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 16 Jan 2024 19:46:27 +0100 Subject: [PATCH 242/387] update vep --- ...ces_v1.txt => variant_consequences_v2.txt} | 8 +- conf/modules/annotate_genome_snvs.config | 9 +- conf/modules/annotate_mt_snvs.config | 9 +- .../annotate_structural_variants.config | 4 +- conf/test.config | 9 +- conf/test_one_sample.config | 9 +- modules.json | 5 + .../nf-core/ensemblvep/vep/environment.yml | 7 ++ modules/nf-core/ensemblvep/vep/main.nf | 71 ++++++++++++++ modules/nf-core/ensemblvep/vep/meta.yml | 92 +++++++++++++++++++ nextflow_schema.json | 13 ++- subworkflows/local/annotate_genome_snvs.nf | 14 ++- subworkflows/local/annotate_mt_snvs.nf | 33 ++++--- .../local/annotate_structural_variants.nf | 30 +++--- workflows/raredisease.nf | 25 ++++- 15 files changed, 273 insertions(+), 65 deletions(-) rename assets/{variant_consequences_v1.txt => variant_consequences_v2.txt} (95%) create mode 100644 modules/nf-core/ensemblvep/vep/environment.yml create mode 100644 modules/nf-core/ensemblvep/vep/main.nf create mode 100644 modules/nf-core/ensemblvep/vep/meta.yml diff --git a/assets/variant_consequences_v1.txt b/assets/variant_consequences_v2.txt similarity index 95% rename from assets/variant_consequences_v1.txt rename to assets/variant_consequences_v2.txt index 0893a8b9..effe32b1 100644 --- a/assets/variant_consequences_v1.txt +++ b/assets/variant_consequences_v2.txt @@ -6,12 +6,14 @@ frameshift_variant stop_lost start_lost transcript_amplification +feature_elongation +feature_truncation inframe_insertion inframe_deletion missense_variant protein_altering_variant -splice_region_variant splice_donor_5th_base_variant +splice_region_variant splice_donor_region_variant splice_polypyrimidine_tract_variant incomplete_terminal_codon_variant @@ -26,6 +28,7 @@ non_coding_transcript_exon_variant intron_variant NMD_transcript_variant non_coding_transcript_variant +coding_transcript_variant upstream_gene_variant downstream_gene_variant TFBS_ablation @@ -33,7 +36,6 @@ TFBS_amplification TF_binding_site_variant regulatory_region_ablation regulatory_region_amplification -feature_elongation regulatory_region_variant -feature_truncation intergenic_variant +sequence_variant diff --git a/conf/modules/annotate_genome_snvs.config b/conf/modules/annotate_genome_snvs.config index 6697c498..5b1e9928 100644 --- a/conf/modules/annotate_genome_snvs.config +++ b/conf/modules/annotate_genome_snvs.config @@ -79,16 +79,15 @@ process { ext.prefix = { "${vcf.simpleName}_rohann_vcfanno_filter_vep" } ext.args = [ '--dir_plugins vep_cache/Plugins', - '--plugin LoFtool,vep_cache/LoFtool_scores.txt', - '--plugin pLI,vep_cache/pLI_values_107.txt', - '--plugin SpliceAI,snv=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', - '--plugin MaxEntScan,vep_cache/fordownload,SWA,NCSS', + '--plugin LoFtool,LoFtool_scores.txt', + '--plugin pLI,pLI_values_107.txt', + '--plugin SpliceAI,snv=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', '--distance 5000', '--buffer_size 20000', '--format vcf --max_sv_size 248956422', '--appris --biotype --cache --canonical --ccds --compress_output bgzip', '--domains --exclude_predicted --force_overwrite', - '--hgvs --humdiv --no_progress --no_stats --numbers', + '--hgvs --humdiv --no_progress --numbers', '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl', '--uniprot --vcf' ].join(' ') diff --git a/conf/modules/annotate_mt_snvs.config b/conf/modules/annotate_mt_snvs.config index 391a3e71..f0e46836 100644 --- a/conf/modules/annotate_mt_snvs.config +++ b/conf/modules/annotate_mt_snvs.config @@ -20,16 +20,15 @@ process { withName: '.*ANNOTATE_MT_SNVS:ENSEMBLVEP_MT' { ext.args = [ '--dir_plugins vep_cache/Plugins', - '--plugin LoFtool,vep_cache/LoFtool_scores.txt', - '--plugin pLI,vep_cache/pLI_values_107.txt', - '--plugin SpliceAI,snv=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=vep_cache/spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', - '--plugin MaxEntScan,vep_cache/fordownload,SWA,NCSS', + '--plugin LoFtool,LoFtool_scores.txt', + '--plugin pLI,pLI_values_107.txt', + '--plugin SpliceAI,snv=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', '--distance 0', '--buffer_size 20000', '--format vcf --fork 4 --max_sv_size 248956422', '--appris --biotype --cache --canonical --ccds --compress_output bgzip', '--domains --exclude_predicted --force_overwrite', - '--hgvs --humdiv --no_progress --no_stats --numbers', + '--hgvs --humdiv --no_progress --numbers', '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl --vcf', '--uniprot' ].join(' ') diff --git a/conf/modules/annotate_structural_variants.config b/conf/modules/annotate_structural_variants.config index b2ee6218..9f8f5f19 100644 --- a/conf/modules/annotate_structural_variants.config +++ b/conf/modules/annotate_structural_variants.config @@ -46,12 +46,12 @@ process { ext.args = [ '--dir_cache vep_cache', '--dir_plugins vep_cache/Plugins', - '--plugin pLI,vep_cache/pLI_values_107.txt', + '--plugin pLI,pLI_values_107.txt', '--appris --biotype --buffer_size 100 --canonical --cache --ccds', '--compress_output bgzip --distance 5000 --domains', '--exclude_predicted --force_overwrite --format vcf', '--fork 4 --hgvs --humdiv --max_sv_size 248956422 --merged', - '--no_progress --no_stats --numbers --per_gene --polyphen p', + '--no_progress --numbers --per_gene --polyphen p', '--protein --offline --regulatory --sift p', '--symbol --tsl --uniprot --vcf' ].join(' ') diff --git a/conf/test.config b/conf/test.config index d43f319b..5a5fdc39 100644 --- a/conf/test.config +++ b/conf/test.config @@ -56,11 +56,6 @@ params { vcfanno_toml = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_config.toml" vep_cache = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_cache_and_plugins.tar.gz" vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" - vep_cache_version = 107 -} - -process { - withName: '.*FILTERVEP.*' { - container = "docker.io/ensemblorg/ensembl-vep:release_107.0" - } + vep_cache_version = 110 + vep_plugin_files = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_files.csv" } diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index de8436c8..82795453 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -56,11 +56,6 @@ params { vcfanno_toml = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_config.toml" vep_cache = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_cache_and_plugins.tar.gz" vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" - vep_cache_version = 107 -} - -process { - withName: '.*FILTERVEP.*' { - container = "docker.io/ensemblorg/ensembl-vep:release_107.0" - } + vep_cache_version = 110 + vep_plugin_files = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_files.csv" } diff --git a/modules.json b/modules.json index 9a1324fd..eac809df 100644 --- a/modules.json +++ b/modules.json @@ -115,6 +115,11 @@ "git_sha": "29984d70aea47d06f0062a1785d76c357dd40ea9", "installed_by": ["modules"] }, + "ensemblvep/vep": { + "branch": "master", + "git_sha": "214d575774c172062924ad3564b4f66655600730", + "installed_by": ["modules"] + }, "expansionhunter": { "branch": "master", "git_sha": "0260e5d22372eae434816d6970dedf3f5adc0053", diff --git a/modules/nf-core/ensemblvep/vep/environment.yml b/modules/nf-core/ensemblvep/vep/environment.yml new file mode 100644 index 00000000..7a127746 --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/environment.yml @@ -0,0 +1,7 @@ +name: ensemblvep_vep +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::ensembl-vep=110.0 diff --git a/modules/nf-core/ensemblvep/vep/main.nf b/modules/nf-core/ensemblvep/vep/main.nf new file mode 100644 index 00000000..3a2b7423 --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/main.nf @@ -0,0 +1,71 @@ +process ENSEMBLVEP_VEP { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ensembl-vep:110.0--pl5321h2a3209d_0' : + 'biocontainers/ensembl-vep:110.0--pl5321h2a3209d_0' }" + + input: + tuple val(meta), path(vcf), path(custom_extra_files) + val genome + val species + val cache_version + path cache + tuple val(meta2), path(fasta) + path extra_files + + output: + tuple val(meta), path("*.vcf.gz") , optional:true, emit: vcf + tuple val(meta), path("*.tab.gz") , optional:true, emit: tab + tuple val(meta), path("*.json.gz") , optional:true, emit: json + path "*.summary.html" , emit: report + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def file_extension = args.contains("--vcf") ? 'vcf' : args.contains("--json")? 'json' : args.contains("--tab")? 'tab' : 'vcf' + def compress_cmd = args.contains("--compress_output") ? '' : '--compress_output bgzip' + def prefix = task.ext.prefix ?: "${meta.id}" + def dir_cache = cache ? "\${PWD}/${cache}" : "/.vep" + def reference = fasta ? "--fasta $fasta" : "" + """ + vep \\ + -i $vcf \\ + -o ${prefix}.${file_extension}.gz \\ + $args \\ + $compress_cmd \\ + $reference \\ + --assembly $genome \\ + --species $species \\ + --cache \\ + --cache_version $cache_version \\ + --dir_cache $dir_cache \\ + --fork $task.cpus \\ + --stats_file ${prefix}.summary.html \\ + + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ensemblvep: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.vcf.gz + touch ${prefix}.tab.gz + touch ${prefix}.json.gz + touch ${prefix}.summary.html + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ensemblvep: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/ensemblvep/vep/meta.yml b/modules/nf-core/ensemblvep/vep/meta.yml new file mode 100644 index 00000000..d8ff8d14 --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/meta.yml @@ -0,0 +1,92 @@ +name: ensemblvep_vep +description: Ensembl Variant Effect Predictor (VEP). The output-file-format is controlled through `task.ext.args`. +keywords: + - annotation + - vcf + - json + - tab +tools: + - ensemblvep: + description: | + VEP determines the effect of your variants (SNPs, insertions, deletions, CNVs + or structural variants) on genes, transcripts, and protein sequence, as well as regulatory regions. + homepage: https://www.ensembl.org/info/docs/tools/vep/index.html + documentation: https://www.ensembl.org/info/docs/tools/vep/script/index.html + licence: ["Apache-2.0"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: | + vcf to annotate + - custom_extra_files: + type: file + description: | + extra sample-specific files to be used with the `--custom` flag to be configured with ext.args + (optional) + - genome: + type: string + description: | + which genome to annotate with + - species: + type: string + description: | + which species to annotate with + - cache_version: + type: integer + description: | + which version of the cache to annotate with + - cache: + type: file + description: | + path to VEP cache (optional) + - meta2: + type: map + description: | + Groovy Map containing fasta reference information + e.g. [ id:'test' ] + - fasta: + type: file + description: | + reference FASTA file (optional) + pattern: "*.{fasta,fa}" + - extra_files: + type: file + description: | + path to file(s) needed for plugins (optional) +output: + - vcf: + type: file + description: | + annotated vcf (optional) + pattern: "*.ann.vcf.gz" + - tab: + type: file + description: | + tab file with annotated variants (optional) + pattern: "*.ann.tab.gz" + - json: + type: file + description: | + json file with annotated variants (optional) + pattern: "*.ann.json.gz" + - report: + type: file + description: VEP report file + pattern: "*.html" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@maxulysse" + - "@matthdsm" + - "@nvnieuwk" +maintainers: + - "@maxulysse" + - "@matthdsm" + - "@nvnieuwk" diff --git a/nextflow_schema.json b/nextflow_schema.json index 1b1b7641..d29a9320 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -374,6 +374,15 @@ "help_text": "If no directory path is passed, vcf files will not be annotated by vep.", "fa_icon": "fas fa-folder-open" }, + "vep_plugin_files": { + "type": "string", + "exists": true, + "format": "file-path", + "description": "Databases used by both named and custom plugins to annotate variants.", + "fa_icon": "fas fa-file-csv", + "help_text": "Path to a file containing the absolute paths to databases and their indices used by VEP's custom and named plugins resources defined within the vcfanno toml file. One line per resource.", + "mimetype": "text/csv" + }, "vep_filters": { "type": "string", "exists": true, @@ -557,10 +566,10 @@ "properties": { "vep_cache_version": { "type": "integer", - "default": 107, + "default": 110, "description": "Specify the version of the VEP cache provided to the `--vep_cache` option.", "fa_icon": "fas fa-align-center", - "enum": [107] + "enum": [107, 110] } } }, diff --git a/subworkflows/local/annotate_genome_snvs.nf b/subworkflows/local/annotate_genome_snvs.nf index 334b7d4b..291d3acd 100644 --- a/subworkflows/local/annotate_genome_snvs.nf +++ b/subworkflows/local/annotate_genome_snvs.nf @@ -11,7 +11,7 @@ include { UPD as UPD_SITES } from '../../modules/nf-core/up include { UPD as UPD_REGIONS } from '../../modules/nf-core/upd/main' include { CHROMOGRAPH as CHROMOGRAPH_SITES } from '../../modules/nf-core/chromograph/main' include { CHROMOGRAPH as CHROMOGRAPH_REGIONS } from '../../modules/nf-core/chromograph/main' -include { ENSEMBLVEP as ENSEMBLVEP_SNV } from '../../modules/local/ensemblvep/main' +include { ENSEMBLVEP_VEP as ENSEMBLVEP_SNV } from '../../modules/nf-core/ensemblvep/vep/main' include { TABIX_BGZIPTABIX as ZIP_TABIX_ROHCALL } from '../../modules/nf-core/tabix/bgziptabix/main' include { TABIX_BGZIPTABIX as ZIP_TABIX_VCFANNO } from '../../modules/nf-core/tabix/bgziptabix/main' include { TABIX_TABIX as TABIX_VEP } from '../../modules/nf-core/tabix/tabix/main' @@ -36,6 +36,7 @@ workflow ANNOTATE_GENOME_SNVS { ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_gnomad_af // channel: [optional] [ path(tab), path(tbi) ] ch_split_intervals // channel: [mandatory] [ path(intervals) ] + ch_vep_extra_files // channel: [mandatory] [ path(files) ] main: ch_cadd_vcf = Channel.empty() @@ -115,20 +116,23 @@ workflow ANNOTATE_GENOME_SNVS { } .set { ch_for_mix } - ch_vep_in = ch_for_mix.selvar.mix(ch_for_mix.cadd) + ch_for_mix.selvar.mix(ch_for_mix.cadd) + .map { meta, vcf -> return [meta, vcf, []]} + .set { ch_vep_in } + // Annotating with ensembl Vep ENSEMBLVEP_SNV( ch_vep_in, - ch_genome_fasta, val_vep_genome, "homo_sapiens", val_vep_cache_version, ch_vep_cache, - [] + ch_genome_fasta, + ch_vep_extra_files ) - ENSEMBLVEP_SNV.out.vcf_gz + ENSEMBLVEP_SNV.out.vcf .map { meta, vcf -> [meta - meta.subMap('scatterid'), vcf] } .set { ch_vep_out } diff --git a/subworkflows/local/annotate_mt_snvs.nf b/subworkflows/local/annotate_mt_snvs.nf index 8f7c24eb..e1ed903a 100644 --- a/subworkflows/local/annotate_mt_snvs.nf +++ b/subworkflows/local/annotate_mt_snvs.nf @@ -2,13 +2,13 @@ // Annotate MT // -include { TABIX_TABIX as TABIX_TABIX_MT } from '../../modules/nf-core/tabix/tabix/main' -include { ENSEMBLVEP as ENSEMBLVEP_MT } from '../../modules/local/ensemblvep/main' -include { HAPLOGREP2_CLASSIFY as HAPLOGREP2_CLASSIFY_MT } from '../../modules/nf-core/haplogrep2/classify/main' -include { VCFANNO as VCFANNO_MT } from '../../modules/nf-core/vcfanno/main' -include { ANNOTATE_CADD } from './annotation/annotate_cadd' -include { TABIX_BGZIPTABIX as ZIP_TABIX_HMTNOTE } from '../../modules/nf-core/tabix/bgziptabix/main' -include { HMTNOTE_ANNOTATE } from '../../modules/nf-core/hmtnote/annotate/main' +include { TABIX_TABIX as TABIX_TABIX_MT } from '../../modules/nf-core/tabix/tabix/main' +include { ENSEMBLVEP_VEP as ENSEMBLVEP_MT } from '../../modules/nf-core/ensemblvep/vep/main' +include { HAPLOGREP2_CLASSIFY as HAPLOGREP2_CLASSIFY_MT } from '../../modules/nf-core/haplogrep2/classify/main' +include { VCFANNO as VCFANNO_MT } from '../../modules/nf-core/vcfanno/main' +include { ANNOTATE_CADD } from './annotation/annotate_cadd' +include { TABIX_BGZIPTABIX as ZIP_TABIX_HMTNOTE } from '../../modules/nf-core/tabix/bgziptabix/main' +include { HMTNOTE_ANNOTATE } from '../../modules/nf-core/hmtnote/annotate/main' workflow ANNOTATE_MT_SNVS { take: @@ -22,6 +22,8 @@ workflow ANNOTATE_MT_SNVS { val_vep_genome // string: [mandatory] GRCh37 or GRCh38 val_vep_cache_version // string: [mandatory] 107 ch_vep_cache // channel: [mandatory] [ path(cache) ] + ch_vep_cache // channel: [mandatory] [ path(cache) ] + ch_vep_extra_files // channel: [mandatory] [ path(files) ] main: ch_cadd_vcf = Channel.empty() @@ -49,22 +51,27 @@ workflow ANNOTATE_MT_SNVS { return [it[0], it[2]] } .set { ch_for_mix } - ch_vep_in = ch_for_mix.merged.mix(ch_for_mix.cadd) + + ch_for_mix.merged.mix(ch_for_mix.cadd) + .tap { ch_haplogrep_in } + .map { meta, vcf -> return [meta, vcf, []]} + .set { ch_vep_in } + // Annotating with ensembl Vep ENSEMBLVEP_MT( ch_vep_in, - ch_genome_fasta, val_vep_genome, "homo_sapiens", val_vep_cache_version, ch_vep_cache, - [] + ch_genome_fasta, + ch_vep_extra_files ) // Running vcfanno - TABIX_TABIX_MT(ENSEMBLVEP_MT.out.vcf_gz) - ENSEMBLVEP_MT.out.vcf_gz + TABIX_TABIX_MT(ENSEMBLVEP_MT.out.vcf) + ENSEMBLVEP_MT.out.vcf .join(TABIX_TABIX_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) .map { meta, vcf, tbi -> return [meta, vcf, tbi, []]} .set { ch_in_vcfanno } @@ -84,7 +91,7 @@ workflow ANNOTATE_MT_SNVS { ch_tbi_out = ZIP_TABIX_HMTNOTE.out.gz_tbi.map{meta, vcf, tbi -> return [meta, tbi] } // Running haplogrep2 - HAPLOGREP2_CLASSIFY_MT(ch_vep_in, "vcf.gz") + HAPLOGREP2_CLASSIFY_MT(ch_haplogrep_in, "vcf.gz") ch_versions = ch_versions.mix(ENSEMBLVEP_MT.out.versions) ch_versions = ch_versions.mix(TABIX_TABIX_MT.out.versions) diff --git a/subworkflows/local/annotate_structural_variants.nf b/subworkflows/local/annotate_structural_variants.nf index 6766a73c..d2d42027 100644 --- a/subworkflows/local/annotate_structural_variants.nf +++ b/subworkflows/local/annotate_structural_variants.nf @@ -2,12 +2,12 @@ // A subworkflow to annotate structural variants. // -include { SVDB_QUERY as SVDB_QUERY_DB } from '../../modules/nf-core/svdb/query/main' -include { SVDB_QUERY as SVDB_QUERY_BEDPE } from '../../modules/nf-core/svdb/query/main' -include { PICARD_SORTVCF } from '../../modules/nf-core/picard/sortvcf/main' -include { BCFTOOLS_VIEW } from '../../modules/nf-core/bcftools/view/main' -include { ENSEMBLVEP as ENSEMBLVEP_SV } from '../../modules/local/ensemblvep/main' -include { TABIX_TABIX as TABIX_VEP } from '../../modules/nf-core/tabix/tabix/main' +include { SVDB_QUERY as SVDB_QUERY_DB } from '../../modules/nf-core/svdb/query/main' +include { SVDB_QUERY as SVDB_QUERY_BEDPE } from '../../modules/nf-core/svdb/query/main' +include { PICARD_SORTVCF } from '../../modules/nf-core/picard/sortvcf/main' +include { BCFTOOLS_VIEW } from '../../modules/nf-core/bcftools/view/main' +include { ENSEMBLVEP_VEP as ENSEMBLVEP_SV } from '../../modules/nf-core/ensemblvep/vep/main' +include { TABIX_TABIX as TABIX_VEP } from '../../modules/nf-core/tabix/tabix/main' workflow ANNOTATE_STRUCTURAL_VARIANTS { @@ -20,6 +20,7 @@ workflow ANNOTATE_STRUCTURAL_VARIANTS { ch_vep_cache // channel: [mandatory] [ path(cache) ] ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] + ch_vep_extra_files // channel: [mandatory] [ path(files) ] main: ch_versions = Channel.empty() @@ -97,18 +98,21 @@ workflow ANNOTATE_STRUCTURAL_VARIANTS { PICARD_SORTVCF.out.vcf.map { meta, vcf -> return [meta,vcf,[]] }.set { ch_sortvcf } BCFTOOLS_VIEW(ch_sortvcf, [], [], []) + .vcf + .map { meta, vcf -> return [meta, vcf, []]} + .set { ch_vep_in } ENSEMBLVEP_SV( - BCFTOOLS_VIEW.out.vcf, - ch_genome_fasta, + ch_vep_in, val_vep_genome, "homo_sapiens", val_vep_cache_version, ch_vep_cache, - [] + ch_genome_fasta, + ch_vep_extra_files ) - TABIX_VEP (ENSEMBLVEP_SV.out.vcf_gz) + TABIX_VEP (ENSEMBLVEP_SV.out.vcf) ch_versions = ch_versions.mix(SVDB_QUERY_DB.out.versions) ch_versions = ch_versions.mix(SVDB_QUERY_BEDPE.out.versions) @@ -118,7 +122,7 @@ workflow ANNOTATE_STRUCTURAL_VARIANTS { ch_versions = ch_versions.mix(TABIX_VEP.out.versions) emit: - vcf_ann = ENSEMBLVEP_SV.out.vcf_gz // channel: [ val(meta), path(vcf) ] - tbi = TABIX_VEP.out.tbi // channel: [ val(meta), path(tbi) ] - versions = ch_versions // channel: [ path(versions.yml) ] + vcf_ann = ENSEMBLVEP_SV.out.vcf // channel: [ val(meta), path(vcf) ] + tbi = TABIX_VEP.out.tbi // channel: [ val(meta), path(tbi) ] + versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index dac92ee9..2227303c 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -275,7 +275,7 @@ workflow RAREDISEASE { ch_target_intervals = ch_references.target_intervals ch_variant_catalog = params.variant_catalog ? Channel.fromPath(params.variant_catalog).map { it -> [[id:it[0].simpleName],it]}.collect() : Channel.value([[],[]]) - ch_variant_consequences = Channel.fromPath("$projectDir/assets/variant_consequences_v1.txt", checkIfExists: true).collect() + ch_variant_consequences = Channel.fromPath("$projectDir/assets/variant_consequences_v2.txt", checkIfExists: true).collect() ch_vcfanno_resources = params.vcfanno_resources ? Channel.fromPath(params.vcfanno_resources).splitText().map{it -> it.trim()}.collect() : Channel.value([]) ch_vcf2cytosure_blacklist = params.vcf2cytosure_blacklist ? Channel.fromPath(params.vcf2cytosure_blacklist).collect() @@ -286,6 +286,8 @@ workflow RAREDISEASE { : Channel.value([]) ch_vep_cache = ( params.vep_cache && params.vep_cache.endsWith("tar.gz") ) ? ch_references.vep_resources : ( params.vep_cache ? Channel.fromPath(params.vep_cache).collect() : Channel.value([]) ) + ch_vep_extra_files_unsplit = params.vep_plugin_files ? Channel.fromPath(params.vep_plugin_files).collect() + : Channel.value([]) ch_vep_filters = params.vep_filters ? Channel.fromPath(params.vep_filters).collect() : Channel.value([]) ch_versions = ch_versions.mix(ch_references.versions) @@ -298,11 +300,25 @@ workflow RAREDISEASE { ch_svcaller_priority = Channel.value(["tiddit", "manta", "gcnvcaller", "cnvnator"]) } + // Read and store paths in the vep_plugin_files file + ch_vep_extra_files_unsplit.splitCsv ( header:true ) + .map { row -> + f = file(row.vep_files[0]) + if(f.isFile() || f.isDirectory()){ + return [f] + } else { + error("\nVep database file ${f} does not exist.") + } + } + .collect() + .set {ch_vep_extra_files} + // Input QC if (!params.skip_fastqc) { FASTQC (ch_reads) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) } + // CREATE CHROMOSOME BED AND INTERVALS SCATTER_GENOME ( ch_genome_dictionary, @@ -425,7 +441,8 @@ workflow RAREDISEASE { params.vep_cache_version, ch_vep_cache, ch_genome_fasta, - ch_genome_dictionary + ch_genome_dictionary, + ch_vep_extra_files ).set {ch_sv_annotate} ch_versions = ch_versions.mix(ch_sv_annotate.versions) @@ -472,7 +489,8 @@ workflow RAREDISEASE { ch_vep_cache, ch_genome_fasta, ch_gnomad_af, - ch_scatter_split_intervals + ch_scatter_split_intervals, + ch_vep_extra_files ).set {ch_snv_annotate} ch_versions = ch_versions.mix(ch_snv_annotate.versions) @@ -519,6 +537,7 @@ workflow RAREDISEASE { params.genome, params.vep_cache_version, ch_vep_cache, + ch_vep_extra_files ).set {ch_mt_annotate} ch_versions = ch_versions.mix(ch_mt_annotate.versions) From 9434630d71cdb4dac710e7c03935cef0faccd2b7 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 16 Jan 2024 20:05:41 +0100 Subject: [PATCH 243/387] update usage --- docs/usage.md | 25 +++++++++++++------------ 1 file changed, 13 insertions(+), 12 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 34776196..6735eb07 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -225,7 +225,7 @@ The mandatory and optional parameters for each category are tabulated below. | vcfanno_resources2 | vcfanno_lua | | vcfanno_toml3 | vep_filters8 | | vep_cache_version | cadd_resources9 | -| vep_cache4 | | +| vep_cache4 | vep_plugin_files10 | | gnomad_af5 | | | score_config_snv6 | | @@ -233,7 +233,7 @@ The mandatory and optional parameters for each category are tabulated below. 2Path to VCF files and their indices used by vcfanno. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/vcfanno_resources.txt).
3Path to a vcfanno configuration file. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/vcfanno_config.toml).
4 VEP caches can be downloaded [here](https://www.ensembl.org/info/docs/tools/vep/script/vep_cache.html#cache). -VEP plugins and associated files may be installed in the cache directory, and the plugin pLI is mandatory to install. +VEP plugins may be installed in the cache directory, and the plugin pLI is mandatory to install. To supply files required by VEP plugins, use `vep_plugin_files` parameter. See example cache [here](https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_cache_and_plugins.tar.gz).
5 GnomAD VCF files can be downloaded from [here](https://gnomad.broadinstitute.org/downloads). The option `gnomad_af` expects a tab-delimited file with no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/gnomad_reformated.tab.gz).
@@ -241,6 +241,7 @@ no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sampl 7Used by GENMOD while modeling the variants. Contains a list of loci that show [reduced penetrance](https://medlineplus.gov/genetics/understanding/inheritance/penetranceexpressivity/) in people. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/reduced_penetrance.tsv).
8 This file contains a list of candidate genes (with [HGNC](https://www.genenames.org/) IDs) that is used to split the variants into canditate variants and research variants. Research variants contain all the variants, while candidate variants are a subset of research variants and are associated with candidate genes. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/hgnc.txt). Not required if --skip_vep_filter is set to true.
9Path to a folder containing cadd annotations. Equivalent of the data/annotations/ folder described [here](https://github.com/kircherlab/CADD-scripts/#manual-installation), and it is used to calculate CADD scores for small indels.
+10A CSV file that describes the files used by VEP's named and custom plugins. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/vep_files.csv).
> NB: We use CADD only to annotate small indels. To annotate SNVs with precomputed CADD scores, pass the file containing CADD scores as a resource to vcfanno instead. Files containing the precomputed CADD scores for SNVs can be downloaded from [here](https://cadd.gs.washington.edu/download) (description: "All possible SNVs of GRCh3<7/8>/hg3<7/8>") @@ -251,22 +252,22 @@ no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sampl | genome | reduced_penetrance | | svdb_query_dbs/svdb_query_bedpedbs1 | | | vep_cache_version | vep_filters | -| vep_cache | | +| vep_cache | vep_plugin_files | | score_config_sv | | 1 A CSV file that describes the databases (VCFs or BEDPEs) used by SVDB for annotating structural variants. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/svdb_querydb_files.csv). Information about the column headers can be found [here](https://github.com/J35P312/SVDB#Query). ##### 9. Mitochondrial annotation -| Mandatory | Optional | -| ----------------- | ----------- | -| genome | vep_filters | -| mito_name | | -| vcfanno_resources | | -| vcfanno_toml | | -| vep_cache_version | | -| vep_cache | | -| score_config_mt | | +| Mandatory | Optional | +| ----------------- | ---------------- | +| genome | vep_filters | +| mito_name | vep_plugin_files | +| vcfanno_resources | | +| vcfanno_toml | | +| vep_cache_version | | +| vep_cache | | +| score_config_mt | | #### Run the pipeline From dc4e176116c288a09b735ea7f1416d7711d71793 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 16 Jan 2024 20:21:44 +0100 Subject: [PATCH 244/387] update changelog --- CHANGELOG.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 0878e5c8..dda4ef6f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -23,6 +23,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - GATK CNVCaller uses segments instead of intervals, filters out "reference" segments between the calls, and fixes a bug with how `ch_readcount_intervals` was handled [#472](https://github.com/nf-core/raredisease/pull/472) - bwa aligner [#474](https://github.com/nf-core/raredisease/pull/474) - Add FOUND_IN tag, which mentions the variant caller that found the mutation, in the INFO column of the vcf files [#471](https://github.com/nf-core/raredisease/pull/471) +- A new parameter `vep_plugin_files` to supply files required by vep plugins [#482](https://github.com/nf-core/raredisease/pull/482) ### `Changed` @@ -42,6 +43,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Changed the name of the parameter from `skip_cnv_calling` to `skip_germlinecnvcaller` [#435](https://github.com/nf-core/raredisease/pull/435) - Check SVDB query input files for existence and correct format [#476](https://github.com/nf-core/raredisease/pull/476) - Change hardcoded platform value to params.platform in align_MT.config [#475](https://github.com/nf-core/raredisease/pull/475) +- Installed the nf-core version of ensemblvep/vep module [#482](https://github.com/nf-core/raredisease/pull/482) ### `Fixed` From de9ff795de76bedf89cec51310dff3124b43cdf9 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 16 Jan 2024 20:30:50 +0100 Subject: [PATCH 245/387] fix lint error --- nextflow.config | 3 +++ 1 file changed, 3 insertions(+) diff --git a/nextflow.config b/nextflow.config index a6551e77..0ff60379 100644 --- a/nextflow.config +++ b/nextflow.config @@ -39,6 +39,9 @@ params { cadd_resources = null platform = 'illumina' + // Annotation + vep_cache_version = 110 + // Bam_qc ngsbits_samplegender_method = 'xy' From 3ee9fed33c5c14af78c88b7e30f76d51f70d9e61 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 16 Jan 2024 20:36:42 +0100 Subject: [PATCH 246/387] fix lint error --- main.nf | 2 +- nextflow.config | 3 --- 2 files changed, 1 insertion(+), 4 deletions(-) diff --git a/main.nf b/main.nf index 0ebf4e51..d87f9e02 100644 --- a/main.nf +++ b/main.nf @@ -54,7 +54,7 @@ params.vcfanno_toml = WorkflowMain.getGenomeAttribute(params, params.vcfanno_lua = WorkflowMain.getGenomeAttribute(params, 'vcfanno_lua') params.vep_cache = WorkflowMain.getGenomeAttribute(params, 'vep_cache') params.vep_cache_version = WorkflowMain.getGenomeAttribute(params, 'vep_cache_version') - +params.vep_plugin_files = WorkflowMain.getGenomeAttribute(params, 'vep_plugin_files') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ VALIDATE & PRINT PARAMETER SUMMARY diff --git a/nextflow.config b/nextflow.config index 0ff60379..a6551e77 100644 --- a/nextflow.config +++ b/nextflow.config @@ -39,9 +39,6 @@ params { cadd_resources = null platform = 'illumina' - // Annotation - vep_cache_version = 110 - // Bam_qc ngsbits_samplegender_method = 'xy' From 098678592b697f107cc0d0e1f7ea82ca6ce961c9 Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 18 Jan 2024 11:38:55 +0100 Subject: [PATCH 247/387] adding mobil element annotation --- bin/add_most_severe_pli.py | 3 +- conf/modules/annotate_mobile_elements.config | 82 +++++++++++++ conf/modules/call_mobile_elements.config | 3 +- nextflow.config | 8 +- nextflow_schema.json | 9 ++ .../local/annotate_consequence_pli.nf | 1 + .../local/annotate_mobile_elements.nf | 109 ++++++++++++++++++ subworkflows/local/call_mobile_elements.nf | 36 ++++-- workflows/raredisease.nf | 16 +++ 9 files changed, 250 insertions(+), 17 deletions(-) create mode 100644 conf/modules/annotate_mobile_elements.config create mode 100644 subworkflows/local/annotate_mobile_elements.nf diff --git a/bin/add_most_severe_pli.py b/bin/add_most_severe_pli.py index ce36768e..b578fe28 100755 --- a/bin/add_most_severe_pli.py +++ b/bin/add_most_severe_pli.py @@ -49,6 +49,7 @@ def construct_most_severe_pli_info(line: str, pli_ind: int) -> list: for field in info_fields: if field.startswith("CSQ="): transcripts = field.split("CSQ=")[1].split(",") + break pli_values = parse_vep_transcripts(transcripts, pli_ind) try: pli_max = max(pli_values) @@ -80,7 +81,7 @@ def write_pli_annotated_vcf(file_in: TextIO, file_out: TextIO): for line in file_in: if line.startswith("#"): file_out.write(line) - if line.startswith("##INFO=\n' diff --git a/conf/modules/annotate_mobile_elements.config b/conf/modules/annotate_mobile_elements.config new file mode 100644 index 00000000..6cf5c0b4 --- /dev/null +++ b/conf/modules/annotate_mobile_elements.config @@ -0,0 +1,82 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. + ext.when = Conditional clause +---------------------------------------------------------------------------------------- +*/ + +// +// Mobile element variant annotation options +// + +process { + + withName: '.*ANNOTATE_MOBILE_ELEMENTS:.*' { + publishDir = [ + enabled: false + ] + } + + withName: '.*ANNOTATE_MOBILE_ELEMENTS:SVDB_QUERY_DB' { + ext.args = { [ + '--bnd_distance 150', + '--overlap -1' + ].join(' ') } + ext.prefix = { "${meta.id}_me_svdb" } + } + + withName: '.*ANNOTATE_MOBILE_ELEMENTS:PICARD_SORTVCF' { + ext.prefix = { "${meta.id}_sortvcf" } + } + + withName: '.*ANNOTATE_MOBILE_ELEMENTS:ENSEMBLVEP_ME' { + ext.args = { [ + '--dir_cache vep_cache', + '--dir_plugins vep_cache/Plugins', + '--plugin pLI,vep_cache/pLI_values_107.txt', + '--appris --biotype --buffer_size 100 --canonical --cache --ccds', + '--compress_output bgzip --distance 5000 --domains', + '--exclude_predicted --force_overwrite --format vcf', + '--fork 4 --hgvs --humdiv --max_sv_size 248956422 --merged', + '--no_progress --no_stats --numbers --per_gene --polyphen p', + '--protein --offline --regulatory --sift p', + '--symbol --tsl --uniprot --vcf' + ].join(' ') } + ext.prefix = { "${meta.id}_svdbquery_vep" } + } + + withName: '.*ANNOTATE_MOBILE_ELEMENTS:BCFTOOLS_VIEW_FILTER' { + // extend filter with arguments such as --exclude 'INFO/swegen_sva_FRQ > 0.1' + ext.args = { "--apply-filters PASS" } + ext.prefix = { "${meta.id}_filter" } + } + + withName: '.*ANNOTATE_MOBILE_ELEMENTS:FILTERVEP_ME' { + ext.when = !params.skip_vep_filter + ext.prefix = { "${meta.id}_me_${meta.set}" } + ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } + } + + withName: '.*ANNOTATE_MOBILE_ELEMENTS:ANNOTATE_CSQ_PLI_ME:ADD_MOST_SEVERE_CSQ' { + ext.prefix = { "${meta.id}_me_csq_${meta.set}" } + } + + withName: '.*ANNOTATE_MOBILE_ELEMENTS:ANNOTATE_CSQ_PLI_ME:ADD_MOST_SEVERE_PLI' { + ext.prefix = { "${meta.id}_me_pli_${meta.set}" } + } + + withName: '.*ANNOTATE_MOBILE_ELEMENTS:ANNOTATE_CSQ_PLI_ME:TABIX_BGZIPTABIX' { + ext.prefix = { "${meta.id}_me_annotated_${meta.set}" } + publishDir = [ + path: { "${params.outdir}/annotate_mobile_elements" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } +} diff --git a/conf/modules/call_mobile_elements.config b/conf/modules/call_mobile_elements.config index fabc6b94..f3622869 100644 --- a/conf/modules/call_mobile_elements.config +++ b/conf/modules/call_mobile_elements.config @@ -43,7 +43,7 @@ process { } withName: '.*CALL_MOBILE_ELEMENTS:BCFTOOLS_SORT_ME' { - ext.args = { '--output-type z' } + ext.args = { '--output-type z --temp-dir ./' } ext.prefix = { "${meta.id}_${meta.interval}_retroseq_sort" } } @@ -69,5 +69,4 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } - } diff --git a/nextflow.config b/nextflow.config index a6551e77..fb27d0a6 100644 --- a/nextflow.config +++ b/nextflow.config @@ -43,9 +43,10 @@ params { ngsbits_samplegender_method = 'xy' // File params - svdb_query_bedpedbs = null - svdb_query_dbs = null - mobile_element_references = null + svdb_query_bedpedbs = null + svdb_query_dbs = null + mobile_element_references = null + mobile_element_svdb_resources = null // Alignment aligner = 'bwamem2' @@ -331,6 +332,7 @@ includeConfig 'conf/modules/call_sv_manta.config' includeConfig 'conf/modules/call_sv_tiddit.config' includeConfig 'conf/modules/postprocess_MT_calls.config' includeConfig 'conf/modules/call_mobile_elements.config' +includeConfig 'conf/modules/annotate_mobile_elements.config' // Function to ensure that resource requirements don't go beyond // a maximum limit diff --git a/nextflow_schema.json b/nextflow_schema.json index 1b1b7641..41fc279b 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -223,6 +223,15 @@ "format": "file-path", "schema": "assets/mobile_element_references_schema.json" }, + "mobile_element_svdb_resources": { + "type": "string", + "description": "File with mobile element allele frequency references", + "help_text": "Path to csv file listing files containing mobile element allele frequencies in reference populations. \nFormat: ,,,,", + "fa_icon": "fas fa-file", + "pattern": "^\\S+\\.csv$", + "mimetype": "text/csv", + "schema": "assets/svdb_query_vcf_schema.json" + }, "ml_model": { "type": "string", "exists": true, diff --git a/subworkflows/local/annotate_consequence_pli.nf b/subworkflows/local/annotate_consequence_pli.nf index aa0f257e..b84d948f 100644 --- a/subworkflows/local/annotate_consequence_pli.nf +++ b/subworkflows/local/annotate_consequence_pli.nf @@ -26,5 +26,6 @@ workflow ANNOTATE_CSQ_PLI { emit: vcf_ann = TABIX_BGZIPTABIX.out.gz_tbi.map { meta, vcf, tbi -> return [ meta, vcf ] }.collect() // channel: [ val(meta), path(vcf) ] + tbi_ann = TABIX_BGZIPTABIX.out.gz_tbi.map { meta, vcf, tbi -> return [ meta, tbi ] }.collect() // channel: [ val(meta), path(tbi) ] versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/subworkflows/local/annotate_mobile_elements.nf b/subworkflows/local/annotate_mobile_elements.nf new file mode 100644 index 00000000..d07d6aaa --- /dev/null +++ b/subworkflows/local/annotate_mobile_elements.nf @@ -0,0 +1,109 @@ +// +// A subworkflow to annotate structural variants. +// + +include { SVDB_QUERY as SVDB_QUERY_DB } from '../../modules/nf-core/svdb/query/main' +include { PICARD_SORTVCF } from '../../modules/nf-core/picard/sortvcf/main' +include { ENSEMBLVEP as ENSEMBLVEP_ME } from '../../modules/local/ensemblvep/main' +include { ENSEMBLVEP_FILTERVEP as FILTERVEP_ME } from '../../modules/nf-core/ensemblvep/filtervep' +include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_FILTER } from '../../modules/nf-core/bcftools/view/main' +include { TABIX_BGZIPTABIX as BGZIP_TABIX_ME } from '../../modules/nf-core/tabix/bgziptabix/main' + +include { ANNOTATE_CSQ_PLI as ANNOTATE_CSQ_PLI_ME } from '../../subworkflows/local/annotate_consequence_pli.nf' + +workflow ANNOTATE_MOBILE_ELEMENTS { + + take: + ch_vcf // channel: [mandatory] [ val(meta), path(vcf) ] + ch_me_svdb_resources // channel: [mandatory] [ path(csv) ] + ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] + ch_vep_cache // channel: [mandatory] [ path(cache) ] + ch_variant_consequences // channel: [mandatory] [ path(consequences) ] + ch_vep_filters // channel: [mandatory] [ path(vep_filter) ] + val_vep_genome // string: [mandatory] GRCh37 or GRCh38 + val_vep_cache_version // string: [mandatory] default: 107 + + main: + ch_versions = Channel.empty() + ch_svdb_dbs = Channel.empty() + + ch_me_svdb_resources + .splitCsv ( header:true ) + .multiMap { row -> + vcf_dbs: row.filename + in_frqs: row.in_freq_info_key + in_occs: row.in_allele_count_info_key + out_frqs: row.out_freq_info_key + out_occs: row.out_allele_count_info_key + } + .set { ch_svdb_dbs } + + SVDB_QUERY_DB ( + ch_vcf, + ch_svdb_dbs.in_occs.toList(), + ch_svdb_dbs.in_frqs.toList(), + ch_svdb_dbs.out_occs.toList(), + ch_svdb_dbs.out_frqs.toList(), + ch_svdb_dbs.vcf_dbs.toList(), + [] + ) + + PICARD_SORTVCF( + SVDB_QUERY_DB.out.vcf, + ch_genome_fasta, + ch_genome_dictionary + ) + + ENSEMBLVEP_ME( + PICARD_SORTVCF.out.vcf, + ch_genome_fasta, + val_vep_genome, + "homo_sapiens", + val_vep_cache_version, + ch_vep_cache, + [] + ) + + ENSEMBLVEP_ME.out.vcf_gz + .map { meta, vcf -> + [ meta, vcf, [] ] + } + .set {ch_bcftools_filter_input } + + BCFTOOLS_VIEW_FILTER ( ch_bcftools_filter_input, [], [], [] ) + + BCFTOOLS_VIEW_FILTER.out.vcf + .multiMap { meta, vcf -> + clinical: [ meta + [ set: "clinical" ], vcf ] + research: [ meta + [ set: "research" ], vcf ] + } + .set { ch_clin_research_vcf } + + FILTERVEP_ME( + ch_clin_research_vcf.clinical, + ch_vep_filters + ) + + ch_clin_research_vcf.research + .mix( FILTERVEP_ME.out.output ) + .set { ch_annotate_csq_pli_me_input } + + + ANNOTATE_CSQ_PLI_ME ( + ch_annotate_csq_pli_me_input, + ch_variant_consequences + ) + + ch_versions = ch_versions.mix(SVDB_QUERY_DB.out.versions) + ch_versions = ch_versions.mix(PICARD_SORTVCF.out.versions) + ch_versions = ch_versions.mix(ENSEMBLVEP_ME.out.versions) + ch_versions = ch_versions.mix(BCFTOOLS_VIEW_FILTER.out.versions) + ch_versions = ch_versions.mix(FILTERVEP_ME.out.versions) + ch_versions = ch_versions.mix(ANNOTATE_CSQ_PLI_ME.out.versions) + + emit: + vcf = ANNOTATE_CSQ_PLI_ME.out.vcf_ann // channel: [ val(meta), path(vcf) ] + tbi = ANNOTATE_CSQ_PLI_ME.out.tbi_ann // channel: [ val(meta), path(tbi) ] + versions = ch_versions // channel: [ path(versions.yml) ] +} diff --git a/subworkflows/local/call_mobile_elements.nf b/subworkflows/local/call_mobile_elements.nf index fd26e6d5..172027da 100644 --- a/subworkflows/local/call_mobile_elements.nf +++ b/subworkflows/local/call_mobile_elements.nf @@ -30,14 +30,16 @@ workflow CALL_MOBILE_ELEMENTS { ch_genome_fai .splitCsv(sep: "\t", elem: 1, limit: 25) .map { meta, fai -> [ fai.first() ] } + .collect() + .map { chr -> [ chr, chr.size() ] } + .transpose() .set { ch_chr } - // Building one bam channel per chromosome and adding interval + // Building one bam channel per chromosome and adding interval and the number of intervals ch_genome_bam_bai .combine(ch_chr) - .map { - meta, bam, bai, chr -> - return [ meta + [interval:chr], bam, bai ] + .map { meta, bam, bai, chr, nr_of_chrs -> + [ meta + [interval:chr, nr_of_intervals: nr_of_chrs], bam, bai ] } .set { ch_genome_bam_bai_interval } @@ -46,7 +48,7 @@ workflow CALL_MOBILE_ELEMENTS { ME_INDEX_SPLIT_ALIGNMENT ( ME_SPLIT_ALIGNMENT.out.bam ) ME_SPLIT_ALIGNMENT.out.bam - .join(ME_INDEX_SPLIT_ALIGNMENT.out.bai, failOnMismatch:true, failOnDuplicate: true) + .join(ME_INDEX_SPLIT_ALIGNMENT.out.bai, failOnMismatch: true, failOnDuplicate: true) .set { ch_retroseq_input } ch_me_references @@ -80,20 +82,32 @@ workflow CALL_MOBILE_ELEMENTS { BCFTOOLS_SORT_ME ( BCFTOOLS_REHEADER_ME.out.vcf ) TABIX_ME_SPLIT ( BCFTOOLS_SORT_ME.out.vcf ) - // Concatenate the chromosome vcfs to sample vcfs + // Preparing channels for input to bcftools concat + // resulting channel [ meta, [ vcf_1, vcf_2, ... ], [ tbi_1, tbi_2, ... ] ] BCFTOOLS_SORT_ME.out.vcf - .map { meta, vcf -> [ meta - meta.subMap('interval'), vcf ] } - .groupTuple(size: 25) + .map { meta, vcf -> + [ groupKey( meta - meta.subMap('interval'), meta.nr_of_intervals ), vcf ] + } + .groupTuple() + .map { meta, vcf -> + [ meta - meta.subMap('nr_of_intervals'), vcf ] + } .set { ch_vcfs } TABIX_ME_SPLIT.out.tbi - .map { meta, tbi -> [ meta - meta.subMap('interval'), tbi ] } - .groupTuple(size: 25) + .map { meta, vcf -> + [ groupKey( meta - meta.subMap('interval'), meta.nr_of_intervals ), vcf ] + } + .groupTuple() + .map { meta, vcf -> + [ meta - meta.subMap('nr_of_intervals'), vcf ] + } .set { ch_tbis } ch_vcfs.join(ch_tbis, failOnMismatch: true) - .set { ch_vcfs_tbis} + .set { ch_vcfs_tbis } + // Concatenate the chromosome vcfs to sample vcfs BCFTOOLS_CONCAT_ME ( ch_vcfs_tbis ) // Merge sample vcfs to a case vcf diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index dac92ee9..881e5bb6 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -119,6 +119,7 @@ include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_MT } from '../subworkflows/local/an include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SNV } from '../subworkflows/local/annotate_consequence_pli' include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SV } from '../subworkflows/local/annotate_consequence_pli' include { ANNOTATE_GENOME_SNVS } from '../subworkflows/local/annotate_genome_snvs' +include { ANNOTATE_MOBILE_ELEMENTS } from '../subworkflows/local/annotate_mobile_elements' include { ANNOTATE_MT_SNVS } from '../subworkflows/local/annotate_mt_snvs' include { ANNOTATE_STRUCTURAL_VARIANTS } from '../subworkflows/local/annotate_structural_variants' include { CALL_REPEAT_EXPANSIONS } from '../subworkflows/local/call_repeat_expansions' @@ -245,6 +246,8 @@ workflow RAREDISEASE { : Channel.empty() ch_me_references = params.mobile_element_references ? Channel.fromSamplesheet("mobile_element_references") : Channel.empty() + ch_me_svdb_resources = params.mobile_element_svdb_resources ? Channel.fromPath(params.mobile_element_svdb_resources) + : Channel.empty() ch_ml_model = params.variant_caller.equals("sentieon") ? Channel.fromPath(params.ml_model).map {it -> [[id:it[0].simpleName], it]}.collect() : Channel.value([[:],[]]) ch_mt_intervals = ch_references.mt_intervals @@ -611,6 +614,19 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(CALL_MOBILE_ELEMENTS.out.versions) + ANNOTATE_MOBILE_ELEMENTS( + CALL_MOBILE_ELEMENTS.out.vcf, + ch_me_svdb_resources, + ch_genome_fasta, + ch_genome_dictionary, + ch_vep_cache, + ch_variant_consequences, + ch_vep_filters, + params.genome, + params.vep_cache_version + ) + ch_versions = ch_versions.mix(ANNOTATE_MOBILE_ELEMENTS.out.versions) + // // MODULE: Pipeline reporting // From 338186d2cc8b7f1ff34addb840de46a2c4751d33 Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 18 Jan 2024 12:42:11 +0100 Subject: [PATCH 248/387] adding test data and updating parameter name --- conf/test.config | 3 ++- conf/test_one_sample.config | 3 ++- conf/test_sentieon.config | 3 ++- nextflow.config | 8 ++++---- nextflow_schema.json | 2 +- workflows/raredisease.nf | 2 +- 6 files changed, 12 insertions(+), 9 deletions(-) diff --git a/conf/test.config b/conf/test.config index d43f319b..99d6a38b 100644 --- a/conf/test.config +++ b/conf/test.config @@ -42,8 +42,9 @@ params { intervals_wgs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/target_wgs.interval_list" intervals_y = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/targetY.interval_list" known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" - mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" + mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" + mobile_element_svdb_annotations = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/svdb_querydb_files.csv" reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" score_config_mt = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" score_config_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index de8436c8..3606b303 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -42,8 +42,9 @@ params { intervals_wgs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/target_wgs.interval_list" intervals_y = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/targetY.interval_list" known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" - mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" + mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" + mobile_element_svdb_annotations = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/svdb_querydb_files.csv" reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" score_config_mt = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" score_config_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" diff --git a/conf/test_sentieon.config b/conf/test_sentieon.config index aca4b755..6084f1df 100644 --- a/conf/test_sentieon.config +++ b/conf/test_sentieon.config @@ -37,8 +37,9 @@ params { intervals_wgs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/target_wgs.interval_list" intervals_y = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/targetY.interval_list" known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" - mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" + mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" + mobile_element_svdb_annotations = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/svdb_querydb_files.csv" reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" score_config_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" score_config_sv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_sv.ini" diff --git a/nextflow.config b/nextflow.config index fb27d0a6..50e0c6aa 100644 --- a/nextflow.config +++ b/nextflow.config @@ -43,10 +43,10 @@ params { ngsbits_samplegender_method = 'xy' // File params - svdb_query_bedpedbs = null - svdb_query_dbs = null - mobile_element_references = null - mobile_element_svdb_resources = null + svdb_query_bedpedbs = null + svdb_query_dbs = null + mobile_element_references = null + mobile_element_svdb_annotations = null // Alignment aligner = 'bwamem2' diff --git a/nextflow_schema.json b/nextflow_schema.json index 41fc279b..6eb22c9f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -223,7 +223,7 @@ "format": "file-path", "schema": "assets/mobile_element_references_schema.json" }, - "mobile_element_svdb_resources": { + "mobile_element_svdb_annotations": { "type": "string", "description": "File with mobile element allele frequency references", "help_text": "Path to csv file listing files containing mobile element allele frequencies in reference populations. \nFormat: ,,,,", diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 881e5bb6..c765e1b0 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -246,7 +246,7 @@ workflow RAREDISEASE { : Channel.empty() ch_me_references = params.mobile_element_references ? Channel.fromSamplesheet("mobile_element_references") : Channel.empty() - ch_me_svdb_resources = params.mobile_element_svdb_resources ? Channel.fromPath(params.mobile_element_svdb_resources) + ch_me_svdb_resources = params.mobile_element_svdb_annotations ? Channel.fromPath(params.mobile_element_svdb_annotations) : Channel.empty() ch_ml_model = params.variant_caller.equals("sentieon") ? Channel.fromPath(params.ml_model).map {it -> [[id:it[0].simpleName], it]}.collect() : Channel.value([[:],[]]) From 5ed33f6d3670ee76f39b7cd7cdb687be6cfc4cdd Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 18 Jan 2024 13:04:30 +0100 Subject: [PATCH 249/387] update changelog and add skip option --- CHANGELOG.md | 4 ++- conf/modules/annotate_mobile_elements.config | 1 + nextflow.config | 1 + nextflow_schema.json | 5 ++++ workflows/raredisease.nf | 30 ++++++++++++-------- 5 files changed, 28 insertions(+), 13 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 0878e5c8..9f2c697e 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -22,7 +22,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New workflow for calling insertion of mobile elements [#440](https://github.com/nf-core/raredisease/pull/440) - GATK CNVCaller uses segments instead of intervals, filters out "reference" segments between the calls, and fixes a bug with how `ch_readcount_intervals` was handled [#472](https://github.com/nf-core/raredisease/pull/472) - bwa aligner [#474](https://github.com/nf-core/raredisease/pull/474) -- Add FOUND_IN tag, which mentions the variant caller that found the mutation, in the INFO column of the vcf files [#471](https://github.com/nf-core/raredisease/pull/471) +- Add FOUND_IN tag, which mentions the variant caller that found the mutation, in the INFO column of the vcf files [#471] +(https://github.com/nf-core/raredisease/pull/471) +- New workflow for annotating mobile elements [#483](https://github.com/nf-core/raredisease/pull/483) ### `Changed` diff --git a/conf/modules/annotate_mobile_elements.config b/conf/modules/annotate_mobile_elements.config index 6cf5c0b4..0e04095c 100644 --- a/conf/modules/annotate_mobile_elements.config +++ b/conf/modules/annotate_mobile_elements.config @@ -18,6 +18,7 @@ process { withName: '.*ANNOTATE_MOBILE_ELEMENTS:.*' { + ext.when = !params.skip_me_annotation publishDir = [ enabled: false ] diff --git a/nextflow.config b/nextflow.config index 50e0c6aa..9634ad31 100644 --- a/nextflow.config +++ b/nextflow.config @@ -32,6 +32,7 @@ params { skip_qualimap = false skip_snv_annotation = false skip_sv_annotation = false + skip_me_annotation = false skip_mt_annotation = false skip_vcf2cytosure = true skip_vep_filter = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 6eb22c9f..853d3774 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -460,6 +460,11 @@ "description": "Specifies whether or not to skip Qualimap.", "fa_icon": "fas fa-toggle-on" }, + "skip_me_annotation": { + "type": "boolean", + "description": "Specifies whether or not to skip annotation of mobile_elements.", + "fa_icon": "fas fa-toggle-on" + }, "skip_mt_annotation": { "type": "boolean", "description": "Specifies whether or not to skip annotation of mitochondrial variants.", diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index c765e1b0..9481b930 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -67,6 +67,10 @@ if (!params.skip_vep_filter) { mandatoryParams += ["vep_filters"] } +if (!params.skip_me_annotation) { + mandatoryParams += ["mobile_element_svdb_annotations"] +} + for (param in mandatoryParams.unique()) { if (params[param] == null) { println("params." + param + " not set.") @@ -614,18 +618,20 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(CALL_MOBILE_ELEMENTS.out.versions) - ANNOTATE_MOBILE_ELEMENTS( - CALL_MOBILE_ELEMENTS.out.vcf, - ch_me_svdb_resources, - ch_genome_fasta, - ch_genome_dictionary, - ch_vep_cache, - ch_variant_consequences, - ch_vep_filters, - params.genome, - params.vep_cache_version - ) - ch_versions = ch_versions.mix(ANNOTATE_MOBILE_ELEMENTS.out.versions) + if (!params.skip_me_annotation) { + ANNOTATE_MOBILE_ELEMENTS( + CALL_MOBILE_ELEMENTS.out.vcf, + ch_me_svdb_resources, + ch_genome_fasta, + ch_genome_dictionary, + ch_vep_cache, + ch_variant_consequences, + ch_vep_filters, + params.genome, + params.vep_cache_version + ) + ch_versions = ch_versions.mix(ANNOTATE_MOBILE_ELEMENTS.out.versions) + } // // MODULE: Pipeline reporting From 33fb5e024feab5f2d33989791ff64b1c2f787721 Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 18 Jan 2024 13:10:54 +0100 Subject: [PATCH 250/387] prettier on changelog --- CHANGELOG.md | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 9f2c697e..93161c13 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -22,8 +22,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New workflow for calling insertion of mobile elements [#440](https://github.com/nf-core/raredisease/pull/440) - GATK CNVCaller uses segments instead of intervals, filters out "reference" segments between the calls, and fixes a bug with how `ch_readcount_intervals` was handled [#472](https://github.com/nf-core/raredisease/pull/472) - bwa aligner [#474](https://github.com/nf-core/raredisease/pull/474) -- Add FOUND_IN tag, which mentions the variant caller that found the mutation, in the INFO column of the vcf files [#471] -(https://github.com/nf-core/raredisease/pull/471) +- Add FOUND_IN tag, which mentions the variant caller that found the mutation, in the INFO column of the vcf files [#471](https://github.com/nf-core/raredisease/pull/471) - New workflow for annotating mobile elements [#483](https://github.com/nf-core/raredisease/pull/483) ### `Changed` From 252bb25e2cdfa43563ceec0bfd86e7179d4a9363 Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 18 Jan 2024 13:52:53 +0100 Subject: [PATCH 251/387] prettier on devcontainer.jsonwq --- .devcontainer/devcontainer.json | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe3..4a9bc5c7 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,11 +18,11 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.linting.pylintPath": "/opt/conda/bin/pylint", }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] - } - } + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], + }, + }, } From 3b096e3e5f8301937f18158e5ac0bda2e9ab5709 Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 18 Jan 2024 14:31:55 +0100 Subject: [PATCH 252/387] updating documentation --- docs/output.md | 30 ++++++++++++++++++++++++++++++ docs/usage.md | 11 +++++++++++ 2 files changed, 41 insertions(+) diff --git a/docs/output.md b/docs/output.md index c80c7a79..885c806e 100644 --- a/docs/output.md +++ b/docs/output.md @@ -492,6 +492,36 @@ We recommend using vcfanno to annotate SNVs with precomputed CADD scores (files
+### Mobile element analysis + +#### Calling mobile elements + +Mobile elements are identified from the bam file using [RetroSeq](https://github.com/tk2/RetroSeq) and the indiviual calls are merged to case VCF using SVDB. + +
+Output files + +- `call_mobile_elements/` + - `_mobile_elements.vcf.gz`: file containing mobile elements. + - `_mobile_elements.vcf.gz.tbi`: index of the file containing mobile elements. + +
+ +#### Annotating mobile elements + +The mobile elements are annotated with allele frequencies and allele counts using SVDB. These annotation files needed are preferably produced from a representative population. Further annoation is done using VEP and the resulting VCF is filtered using bcftools. The default filter is to only keep elements with `PASS` in the filter column but if no other post-processing is done we reccomend supplementing with an exclude expression based on population allele frequencies. The filtering key is dependent on the annotation files used but an example expression could look like this: `--exclude 'INFO/swegen_sva_FRQ > 0.1'`. If a list of HGNC id:s have been supplied with the option `--vep_filters`, variants matching those id:s will be presented in a seperate file using [filter_vep from VEP](https://www.ensembl.org/info/docs/tools/vep/script/vep_filter.html). This option can be disabled using the flag `--skip_vep_filter`. A VCF corresponding to the complete set of variants will also be produced. + +
+Output files + +- `rank_and_filter/` + - `_mobile_elements_annotated_research.vcf.gz`: VCF containting the complete set of annotated mobile elements. + - `_mobile_elements_annotated_research.vcf.gz.tbi`: Index for VCF containting the complete set of annotated mobile elements. + - `_mobile_elements_annotated_clinical.vcf.gz`: VCF containing selected annotated mobile elements. + - `_mobile_elements_annotated_clincial.vcf.gz.tbi`: Index for VCF containing selected annotated mobile elements. + +
+ ### Pipeline information [Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. diff --git a/docs/usage.md b/docs/usage.md index 34776196..9c27621a 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -268,6 +268,17 @@ no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sampl | vep_cache | | | score_config_mt | | +##### 10. Mobile element annotation + +| Mandatory | Optional | +| ------------------------------------------- | ----------- | +| genome | vep_filters | +| mobile_element_svdb_annotations1 | | +| vep_cache_version | | +| vep_cache | | + +1 A CSV file that describes the databases (VCFs) used by SVDB for annotating mobile elements with allele frequencies. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/svdb_querydb_files.csv). + #### Run the pipeline You can directly supply the parameters in the command line (CLI) or use a config file from which the pipeline can import the parameters. From d14976bae0bc7a76b61190e4d432edd1489ad433 Mon Sep 17 00:00:00 2001 From: Anders Jemt Date: Fri, 19 Jan 2024 11:27:01 +0100 Subject: [PATCH 253/387] Apply suggestions from code review MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Lucía Peña-Pérez --- subworkflows/local/annotate_mobile_elements.nf | 2 +- subworkflows/local/call_mobile_elements.nf | 8 ++++---- 2 files changed, 5 insertions(+), 5 deletions(-) diff --git a/subworkflows/local/annotate_mobile_elements.nf b/subworkflows/local/annotate_mobile_elements.nf index d07d6aaa..04b15ad2 100644 --- a/subworkflows/local/annotate_mobile_elements.nf +++ b/subworkflows/local/annotate_mobile_elements.nf @@ -69,7 +69,7 @@ workflow ANNOTATE_MOBILE_ELEMENTS { .map { meta, vcf -> [ meta, vcf, [] ] } - .set {ch_bcftools_filter_input } + .set { ch_bcftools_filter_input } BCFTOOLS_VIEW_FILTER ( ch_bcftools_filter_input, [], [], [] ) diff --git a/subworkflows/local/call_mobile_elements.nf b/subworkflows/local/call_mobile_elements.nf index 172027da..05e6355c 100644 --- a/subworkflows/local/call_mobile_elements.nf +++ b/subworkflows/local/call_mobile_elements.nf @@ -28,7 +28,7 @@ workflow CALL_MOBILE_ELEMENTS { // Building chromosome channels based on fasta index ch_genome_fai - .splitCsv(sep: "\t", elem: 1, limit: 25) + .splitCsv( sep: "\t", elem: 1, limit: 25 ) .map { meta, fai -> [ fai.first() ] } .collect() .map { chr -> [ chr, chr.size() ] } @@ -37,7 +37,7 @@ workflow CALL_MOBILE_ELEMENTS { // Building one bam channel per chromosome and adding interval and the number of intervals ch_genome_bam_bai - .combine(ch_chr) + .combine( ch_chr ) .map { meta, bam, bai, chr, nr_of_chrs -> [ meta + [interval:chr, nr_of_intervals: nr_of_chrs], bam, bai ] } @@ -48,7 +48,7 @@ workflow CALL_MOBILE_ELEMENTS { ME_INDEX_SPLIT_ALIGNMENT ( ME_SPLIT_ALIGNMENT.out.bam ) ME_SPLIT_ALIGNMENT.out.bam - .join(ME_INDEX_SPLIT_ALIGNMENT.out.bai, failOnMismatch: true, failOnDuplicate: true) + .join( ME_INDEX_SPLIT_ALIGNMENT.out.bai, failOnMismatch: true, failOnDuplicate: true ) .set { ch_retroseq_input } ch_me_references @@ -104,7 +104,7 @@ workflow CALL_MOBILE_ELEMENTS { } .set { ch_tbis } - ch_vcfs.join(ch_tbis, failOnMismatch: true) + ch_vcfs.join( ch_tbis, failOnMismatch: true ) .set { ch_vcfs_tbis } // Concatenate the chromosome vcfs to sample vcfs From 76fb0d438d800c9be00d721d08fea322709ff2e0 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sat, 20 Jan 2024 10:36:53 +0100 Subject: [PATCH 254/387] merge me --- conf/modules/annotate_mobile_elements.config | 4 +- conf/test.config | 2 +- conf/test_one_sample.config | 2 +- modules/local/ensemblvep/main.nf | 80 ------------------- modules/local/ensemblvep/meta.yml | 73 ----------------- .../local/annotate_mobile_elements.nf | 27 ++++--- workflows/raredisease.nf | 3 +- 7 files changed, 21 insertions(+), 170 deletions(-) delete mode 100644 modules/local/ensemblvep/main.nf delete mode 100644 modules/local/ensemblvep/meta.yml diff --git a/conf/modules/annotate_mobile_elements.config b/conf/modules/annotate_mobile_elements.config index 0e04095c..442652a3 100644 --- a/conf/modules/annotate_mobile_elements.config +++ b/conf/modules/annotate_mobile_elements.config @@ -40,12 +40,12 @@ process { ext.args = { [ '--dir_cache vep_cache', '--dir_plugins vep_cache/Plugins', - '--plugin pLI,vep_cache/pLI_values_107.txt', + '--plugin pLI,pLI_values_107.txt', '--appris --biotype --buffer_size 100 --canonical --cache --ccds', '--compress_output bgzip --distance 5000 --domains', '--exclude_predicted --force_overwrite --format vcf', '--fork 4 --hgvs --humdiv --max_sv_size 248956422 --merged', - '--no_progress --no_stats --numbers --per_gene --polyphen p', + '--no_progress --numbers --per_gene --polyphen p', '--protein --offline --regulatory --sift p', '--symbol --tsl --uniprot --vcf' ].join(' ') } diff --git a/conf/test.config b/conf/test.config index 1347680d..fd2f873e 100644 --- a/conf/test.config +++ b/conf/test.config @@ -57,6 +57,6 @@ params { vcfanno_toml = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_config.toml" vep_cache = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_cache_and_plugins.tar.gz" vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" - vep_cache_version = 110 + vep_cache_version = 107 vep_plugin_files = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_files.csv" } diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index 12eb9f39..d521a8a3 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -57,6 +57,6 @@ params { vcfanno_toml = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_config.toml" vep_cache = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_cache_and_plugins.tar.gz" vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" - vep_cache_version = 110 + vep_cache_version = 107 vep_plugin_files = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_files.csv" } diff --git a/modules/local/ensemblvep/main.nf b/modules/local/ensemblvep/main.nf deleted file mode 100644 index 81d4191f..00000000 --- a/modules/local/ensemblvep/main.nf +++ /dev/null @@ -1,80 +0,0 @@ -process ENSEMBLVEP { - tag "$meta.id" - label 'process_medium' - - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error("Local VEP module does not support Conda. Please use Docker / Singularity / Podman instead.") - } - - container "docker.io/ensemblorg/ensembl-vep:release_107.0" - - input: - tuple val(meta), path(vcf) - tuple val(meta2), path(fasta) - val genome - val species - val cache_version - path cache - path extra_files - - output: - tuple val(meta), path("*.vcf") , optional:true, emit: vcf - tuple val(meta), path("*.tab") , optional:true, emit: tab - tuple val(meta), path("*.json") , optional:true, emit: json - tuple val(meta), path("*.vcf.gz") , optional:true, emit: vcf_gz - tuple val(meta), path("*.tab.gz") , optional:true, emit: tab_gz - tuple val(meta), path("*.json.gz"), optional:true, emit: json_gz - path "*.summary.html" , optional:true, emit: report - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def file_extension = args.contains("--vcf") ? 'vcf' : args.contains("--json")? 'json' : args.contains("--tab")? 'tab' : 'vcf' - def compress_out = args.contains("--compress_output") ? '.gz' : '' - def prefix = task.ext.prefix ?: "${meta.id}" - def stats_file = args.contains("--no_stats") ? '' : "--stats_file ${prefix}.summary.html" - def dir_cache = cache ? "\${PWD}/${cache}" : "/.vep" - def reference = fasta ? "--fasta $fasta" : "" - - """ - vep \\ - -i $vcf \\ - -o ${prefix}.${file_extension}${compress_out} \\ - $args \\ - $reference \\ - --assembly $genome \\ - --species $species \\ - --cache \\ - --cache_version $cache_version \\ - --dir_cache $dir_cache \\ - --fork $task.cpus \\ - ${stats_file} - - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - ensemblvep: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//') - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.vcf - touch ${prefix}.tab - touch ${prefix}.json - touch ${prefix}.vcf.gz - touch ${prefix}.tab.gz - touch ${prefix}.json.gz - touch ${prefix}.summary.html - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - ensemblvep: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//') - END_VERSIONS - """ -} diff --git a/modules/local/ensemblvep/meta.yml b/modules/local/ensemblvep/meta.yml deleted file mode 100644 index a4dde8a6..00000000 --- a/modules/local/ensemblvep/meta.yml +++ /dev/null @@ -1,73 +0,0 @@ -name: ENSEMBLVEP -description: Ensembl Variant Effect Predictor (VEP). The output-file-format is controlled through `task.ext.args`. -keywords: - - annotation -tools: - - ensemblvep: - description: | - VEP determines the effect of your variants (SNPs, insertions, deletions, CNVs - or structural variants) on genes, transcripts, and protein sequence, as well as regulatory regions. - homepage: https://www.ensembl.org/info/docs/tools/vep/index.html - documentation: https://www.ensembl.org/info/docs/tools/vep/script/index.html - licence: ["Apache-2.0"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - vcf: - type: file - description: | - vcf to annotate - - genome: - type: value - description: | - which genome to annotate with - - species: - type: value - description: | - which species to annotate with - - cache_version: - type: value - description: | - which version of the cache to annotate with - - cache: - type: file - description: | - path to VEP cache (optional) - - fasta: - type: file - description: | - reference FASTA file (optional) - pattern: "*.{fasta,fa}" - - extra_files: - type: tuple - description: | - path to file(s) needed for plugins (optional) -output: - - vcf: - type: file - description: | - annotated vcf (optional) - pattern: "*.ann.vcf" - - tab: - type: file - description: | - tab file with annotated variants (optional) - pattern: "*.ann.tab" - - json: - type: file - description: | - json file with annotated variants (optional) - pattern: "*.ann.json" - - report: - type: file - description: VEP report file - pattern: "*.html" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@maxulysse" diff --git a/subworkflows/local/annotate_mobile_elements.nf b/subworkflows/local/annotate_mobile_elements.nf index 04b15ad2..dc6247bf 100644 --- a/subworkflows/local/annotate_mobile_elements.nf +++ b/subworkflows/local/annotate_mobile_elements.nf @@ -2,13 +2,12 @@ // A subworkflow to annotate structural variants. // -include { SVDB_QUERY as SVDB_QUERY_DB } from '../../modules/nf-core/svdb/query/main' -include { PICARD_SORTVCF } from '../../modules/nf-core/picard/sortvcf/main' -include { ENSEMBLVEP as ENSEMBLVEP_ME } from '../../modules/local/ensemblvep/main' -include { ENSEMBLVEP_FILTERVEP as FILTERVEP_ME } from '../../modules/nf-core/ensemblvep/filtervep' -include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_FILTER } from '../../modules/nf-core/bcftools/view/main' -include { TABIX_BGZIPTABIX as BGZIP_TABIX_ME } from '../../modules/nf-core/tabix/bgziptabix/main' - +include { SVDB_QUERY as SVDB_QUERY_DB } from '../../modules/nf-core/svdb/query/main' +include { PICARD_SORTVCF } from '../../modules/nf-core/picard/sortvcf/main' +include { ENSEMBLVEP_VEP as ENSEMBLVEP_ME } from '../../modules/nf-core/ensemblvep/vep/main' +include { ENSEMBLVEP_FILTERVEP as FILTERVEP_ME } from '../../modules/nf-core/ensemblvep/filtervep' +include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_FILTER } from '../../modules/nf-core/bcftools/view/main' +include { TABIX_BGZIPTABIX as BGZIP_TABIX_ME } from '../../modules/nf-core/tabix/bgziptabix/main' include { ANNOTATE_CSQ_PLI as ANNOTATE_CSQ_PLI_ME } from '../../subworkflows/local/annotate_consequence_pli.nf' workflow ANNOTATE_MOBILE_ELEMENTS { @@ -23,6 +22,7 @@ workflow ANNOTATE_MOBILE_ELEMENTS { ch_vep_filters // channel: [mandatory] [ path(vep_filter) ] val_vep_genome // string: [mandatory] GRCh37 or GRCh38 val_vep_cache_version // string: [mandatory] default: 107 + ch_vep_extra_files // channel: [mandatory] [ path(files) ] main: ch_versions = Channel.empty() @@ -54,18 +54,21 @@ workflow ANNOTATE_MOBILE_ELEMENTS { ch_genome_fasta, ch_genome_dictionary ) + .vcf + .map { meta, vcf -> return [meta, vcf, []]} + .set { ch_vep_in } ENSEMBLVEP_ME( - PICARD_SORTVCF.out.vcf, - ch_genome_fasta, + ch_vep_in, val_vep_genome, "homo_sapiens", val_vep_cache_version, ch_vep_cache, - [] + ch_genome_fasta, + ch_vep_extra_files ) - ENSEMBLVEP_ME.out.vcf_gz + ENSEMBLVEP_ME.out.vcf .map { meta, vcf -> [ meta, vcf, [] ] } @@ -76,7 +79,7 @@ workflow ANNOTATE_MOBILE_ELEMENTS { BCFTOOLS_VIEW_FILTER.out.vcf .multiMap { meta, vcf -> clinical: [ meta + [ set: "clinical" ], vcf ] - research: [ meta + [ set: "research" ], vcf ] + research: [ meta + [ set: "research" ], vcf ] } .set { ch_clin_research_vcf } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index e5781c17..0f2a4fe1 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -647,7 +647,8 @@ workflow RAREDISEASE { ch_variant_consequences, ch_vep_filters, params.genome, - params.vep_cache_version + params.vep_cache_version, + ch_vep_extra_files ) ch_versions = ch_versions.mix(ANNOTATE_MOBILE_ELEMENTS.out.versions) } From 8b14cd9c441b6b9aab4a3455e18db6755f3b6126 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sat, 20 Jan 2024 16:24:04 +0100 Subject: [PATCH 255/387] add rtgtools --- modules.json | 5 + .../nf-core/rtgtools/vcfeval/environment.yml | 7 ++ modules/nf-core/rtgtools/vcfeval/main.nf | 90 ++++++++++++++ modules/nf-core/rtgtools/vcfeval/meta.yml | 112 ++++++++++++++++++ 4 files changed, 214 insertions(+) create mode 100644 modules/nf-core/rtgtools/vcfeval/environment.yml create mode 100644 modules/nf-core/rtgtools/vcfeval/main.nf create mode 100644 modules/nf-core/rtgtools/vcfeval/meta.yml diff --git a/modules.json b/modules.json index 9a1324fd..1c7da4d9 100644 --- a/modules.json +++ b/modules.json @@ -347,6 +347,11 @@ "git_sha": "d73505dd68b27b53b4002e84eea21a2819907562", "installed_by": ["modules"] }, + "rtgtools/vcfeval": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, "samtools/faidx": { "branch": "master", "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe", diff --git a/modules/nf-core/rtgtools/vcfeval/environment.yml b/modules/nf-core/rtgtools/vcfeval/environment.yml new file mode 100644 index 00000000..e3c64996 --- /dev/null +++ b/modules/nf-core/rtgtools/vcfeval/environment.yml @@ -0,0 +1,7 @@ +name: rtgtools_vcfeval +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::rtg-tools=3.12.1 diff --git a/modules/nf-core/rtgtools/vcfeval/main.nf b/modules/nf-core/rtgtools/vcfeval/main.nf new file mode 100644 index 00000000..98f9adb1 --- /dev/null +++ b/modules/nf-core/rtgtools/vcfeval/main.nf @@ -0,0 +1,90 @@ +process RTGTOOLS_VCFEVAL { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/rtg-tools:3.12.1--hdfd78af_0': + 'biocontainers/rtg-tools:3.12.1--hdfd78af_0' }" + + input: + tuple val(meta), path(query_vcf), path(query_vcf_tbi), path(truth_vcf), path(truth_vcf_tbi), path(truth_bed), path(evaluation_bed) + tuple val(meta2), path(sdf) + + output: + tuple val(meta), path("*.tp.vcf.gz") , emit: tp_vcf + tuple val(meta), path("*.tp.vcf.gz.tbi") , emit: tp_tbi + tuple val(meta), path("*.fn.vcf.gz") , emit: fn_vcf + tuple val(meta), path("*.fn.vcf.gz.tbi") , emit: fn_tbi + tuple val(meta), path("*.fp.vcf.gz") , emit: fp_vcf + tuple val(meta), path("*.fp.vcf.gz.tbi") , emit: fp_tbi + tuple val(meta), path("*.tp-baseline.vcf.gz") , emit: baseline_vcf + tuple val(meta), path("*.tp-baseline.vcf.gz.tbi") , emit: baseline_tbi + tuple val(meta), path("*.snp_roc.tsv.gz") , emit: snp_roc + tuple val(meta), path("*.non_snp_roc.tsv.gz") , emit: non_snp_roc + tuple val(meta), path("*.weighted_roc.tsv.gz") , emit: weighted_roc + tuple val(meta), path("*.summary.txt") , emit: summary + tuple val(meta), path("*.phasing.txt") , emit: phasing + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: "" + def prefix = task.ext.prefix ?: "${meta.id}" + def bed_regions = truth_bed ? "--bed-regions=${truth_bed}" : "" + def eval_regions = evaluation_bed ? "--evaluation-regions=${evaluation_bed}" : "" + def truth_index = truth_vcf_tbi ? "" : "rtg index ${truth_vcf}" + def query_index = query_vcf_tbi ? "" : "rtg index ${query_vcf}" + def avail_mem = task.memory.toGiga() + "G" + + """ + ${truth_index} + ${query_index} + + rtg RTG_MEM=$avail_mem vcfeval \\ + ${args} \\ + --baseline=${truth_vcf} \\ + ${bed_regions} \\ + ${eval_regions} \\ + --calls=${query_vcf} \\ + --output=output \\ + --template=${sdf} \\ + --threads=${task.cpus} + + cd output/ + mv done progress .. + for f in * ; do mv "\$f" "../${prefix}.\$f" ; done + cd .. + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + rtg-tools: \$(echo \$(rtg version | head -n 1 | awk '{print \$4}')) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + touch ${prefix}.tp.vcf.gz + touch ${prefix}.tp.vcf.gz.tbi + touch ${prefix}.fn.vcf.gz + touch ${prefix}.fn.vcf.gz.tbi + touch ${prefix}.fp.vcf.gz + touch ${prefix}.fp.vcf.gz.tbi + touch ${prefix}.tp-baseline.vcf.gz + touch ${prefix}.tp-baseline.vcf.gz.tbi + touch ${prefix}.snp_roc.tsv.gz + touch ${prefix}.non_snp_roc.tsv.gz + touch ${prefix}.weighted_roc.tsv.gz + touch ${prefix}.summary.txt + touch ${prefix}.phasing.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + rtg-tools: \$(echo \$(rtg version | head -n 1 | awk '{print \$4}')) + END_VERSIONS + """ +} diff --git a/modules/nf-core/rtgtools/vcfeval/meta.yml b/modules/nf-core/rtgtools/vcfeval/meta.yml new file mode 100644 index 00000000..f67a35c0 --- /dev/null +++ b/modules/nf-core/rtgtools/vcfeval/meta.yml @@ -0,0 +1,112 @@ +name: "rtgtools_vcfeval" +description: The VCFeval tool of RTG tools. It is used to evaluate called variants for agreement with a baseline variant set +keywords: + - benchmarking + - vcf + - rtg-tools +tools: + - "rtgtools": + description: "RealTimeGenomics Tools -- Utilities for accurate VCF comparison and manipulation" + homepage: "https://www.realtimegenomics.com/products/rtg-tools" + documentation: "https://github.com/RealTimeGenomics/rtg-tools" + tool_dev_url: "https://github.com/RealTimeGenomics/rtg-tools" + licence: "['BSD']" +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - query_vcf: + type: file + description: A VCF with called variants to benchmark against the standard + pattern: "*.{vcf,vcf.gz}" + - query_vcf_index: + type: file + description: The index of the called VCF (optional) + pattern: "*.tbi" + - truth_vcf: + type: file + description: A standard VCF to compare against + pattern: "*.{vcf,vcf.gz}" + - truth_vcf_index: + type: file + description: The index of the standard VCF (optional) + pattern: "*.tbi" + - truth_bed: + type: file + description: A BED file containining the strict regions where VCFeval should only evaluate the fully overlapping variants (optional) + pattern: "*.bed" + - evaluation_bed: + type: file + description: A BED file containing the regions where VCFeval will evaluate every fully and partially overlapping variant (optional) + pattern: "*.bed" + - sdf: + type: file + description: The SDF (RTG Sequence Data File) folder of the reference genome +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - tp_vcf: + type: file + description: A VCF file for the true positive variants + pattern: "*.tp.vcf.gz" + - tp_tbi: + type: file + description: The index of the VCF file for the true positive variants + pattern: "*.tp.vcf.gz.tbi" + - fn_vcf: + type: file + description: A VCF file for the false negative variants + pattern: "*.fn.vcf.gz" + - fn_tbi: + type: file + description: The index of the VCF file for the false negative variants + pattern: "*.fn.vcf.gz.tbi" + - fp_vcf: + type: file + description: A VCF file for the false positive variants + pattern: "*.fp.vcf.gz" + - fp_tbi: + type: file + description: The index of the VCF file for the false positive variants + pattern: "*.fp.vcf.gz.tbi" + - baseline_vcf: + type: file + description: A VCF file for the true positive variants from the baseline + pattern: "*.tp-baseline.vcf.gz" + - baseline_tbi: + type: file + description: The index of the VCF file for the true positive variants from the baseline + pattern: "*.tp-baseline.vcf.gz.tbi" + - snp_roc: + type: file + description: TSV files containing ROC data for the SNPs + pattern: "*.snp_roc.tsv.gz" + - non_snp_roc: + type: file + description: TSV files containing ROC data for all variants except SNPs + pattern: "*.non_snp_roc.tsv.gz" + - weighted_roc: + type: file + description: TSV files containing weighted ROC data for all variants + pattern: "*.weighted_snp_roc.tsv.gz" + - summary: + type: file + description: A TXT file containing the summary of the evaluation + pattern: "*.summary.txt" + - phasing: + type: file + description: A TXT file containing the data on the phasing + pattern: "*.phasing.txt" +authors: + - "@nvnieuwk" +maintainers: + - "@nvnieuwk" From da1855f3e4863199f16221bd75918b58b99b8794 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 21 Jan 2024 13:48:42 +0100 Subject: [PATCH 256/387] vcfeval --- assets/rtg_truthvcfs.json | 32 ++++++++++++++++ conf/modules/call_snv.config | 1 - conf/modules/variant_evaluation.config | 30 +++++++++++++++ main.nf | 1 + nextflow.config | 2 + nextflow_schema.json | 16 ++++++++ subworkflows/local/variant_evaluation.nf | 49 ++++++++++++++++++++++++ workflows/raredisease.nf | 22 ++++++++++- 8 files changed, 150 insertions(+), 3 deletions(-) create mode 100644 assets/rtg_truthvcfs.json create mode 100644 conf/modules/variant_evaluation.config create mode 100644 subworkflows/local/variant_evaluation.nf diff --git a/assets/rtg_truthvcfs.json b/assets/rtg_truthvcfs.json new file mode 100644 index 00000000..35e1228e --- /dev/null +++ b/assets/rtg_truthvcfs.json @@ -0,0 +1,32 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/raredisease/master/assets/mobile_element_references_schema.json", + "title": "Schema for RTGTOOLS/VCFEVAL VCFs", + "description": "Schema for the vcfeval's vcf input", + "type": "array", + "items": { + "type": "object", + "properties": { + "samplename": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Samplename cannot contain spaces" + }, + "vcf": { + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.vcf(\\.gz)?$", + "errorMessage": "Path to vcf files cannot contain spaces and must be a .vcf or .vcf.gz file" + }, + "bed": { + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.bed$", + "errorMessage": "Path to bed files cannot contain spaces" + } + }, + "required": ["vcf", "samplename", "bed"] + } +} diff --git a/conf/modules/call_snv.config b/conf/modules/call_snv.config index 17926228..51f7a56f 100644 --- a/conf/modules/call_snv.config +++ b/conf/modules/call_snv.config @@ -22,7 +22,6 @@ process { withName: '.*CALL_SNV:GATK4_SELECTVARIANTS' { ext.args = "--exclude-intervals ${params.mito_name}" ext.prefix = { "${meta.id}_nomito" } - ext.when = { !params.skip_snv_annotation } publishDir = [ path: { "${params.outdir}/call_snv/genome" }, mode: params.publish_dir_mode, diff --git a/conf/modules/variant_evaluation.config b/conf/modules/variant_evaluation.config new file mode 100644 index 00000000..04ea6baa --- /dev/null +++ b/conf/modules/variant_evaluation.config @@ -0,0 +1,30 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. + ext.when = Conditional clause +---------------------------------------------------------------------------------------- +*/ + +// +// Evaluate variants +// + +process { + withName: '.*VARIANT_EVALUATION.*' { + ext.when = { params.run_rtgvcfeval } + } + + withName: '.*VARIANT_EVALUATION:BCFTOOLS_REHEADER' { + beforeScript = { "echo ${meta.id} > ${meta.id}.txt" } + ext.args = { "--samples ${meta.id}.txt" } + ext.args2 = "--output-type z" + ext.when = { params.run_rtgvcfeval } + ext.prefix = { "${meta.id}_truthvcf" } + } +} diff --git a/main.nf b/main.nf index 0ebf4e51..ef782474 100644 --- a/main.nf +++ b/main.nf @@ -39,6 +39,7 @@ params.ngsbits_samplegender_method = WorkflowMain.getGenomeAttribute(params, params.ploidy_model = WorkflowMain.getGenomeAttribute(params, 'ploidy_model') params.reduced_penetrance = WorkflowMain.getGenomeAttribute(params, 'reduced_penetrance') params.readcount_intervals = WorkflowMain.getGenomeAttribute(params, 'readcount_intervals') +params.rtg_truthvcfs = WorkflowMain.getGenomeAttribute(params, 'rtg_truthvcfs') params.sequence_dictionary = WorkflowMain.getGenomeAttribute(params, 'sequence_dictionary') params.score_config_mt = WorkflowMain.getGenomeAttribute(params, 'score_config_mt') params.score_config_snv = WorkflowMain.getGenomeAttribute(params, 'score_config_snv') diff --git a/nextflow.config b/nextflow.config index 9634ad31..ecf0291d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -23,6 +23,7 @@ params { // Main options analysis_type = 'wgs' bait_padding = 100 + run_rtgvcfeval = false save_mapped_as_cram = false skip_eklipse = false skip_fastp = false @@ -334,6 +335,7 @@ includeConfig 'conf/modules/call_sv_tiddit.config' includeConfig 'conf/modules/postprocess_MT_calls.config' includeConfig 'conf/modules/call_mobile_elements.config' includeConfig 'conf/modules/annotate_mobile_elements.config' +includeConfig 'conf/modules/variant_evaluation.config' // Function to ensure that resource requirements don't go beyond // a maximum limit diff --git a/nextflow_schema.json b/nextflow_schema.json index 853d3774..68558759 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -271,6 +271,17 @@ "fa_icon": "fas fa-file-csv", "description": "File with gene ids that have reduced penetrance. For use with genmod" }, + "rtg_truthvcfs": { + "type": "string", + "exists": true, + "format": "file-path", + "description": "Vcf used for evaluating variant calls.", + "fa_icon": "fas fa-file-csv", + "help_text": "Path to comma-separated file containing information about the truth vcf files used by vcfeval.", + "pattern": "^\\S+\\.csv$", + "mimetype": "text/csv", + "schema": "assets/rtg_truthvcfs.json" + }, "save_reference": { "type": "boolean", "description": "If generated by the pipeline save the required indices/references in the results directory.", @@ -425,6 +436,11 @@ "description": "Method selection for ngs-bits samplegender", "fa_icon": "fas fa-align-center" }, + "run_rtgvcfeval": { + "type": "boolean", + "description": "Specifies whether to run rtgtools' vcfeval", + "fa_icon": "fas fa-toggle-on" + }, "save_mapped_as_cram": { "type": "boolean", "description": "Specifies whether to generate and publish alignment files as cram instead of bam", diff --git a/subworkflows/local/variant_evaluation.nf b/subworkflows/local/variant_evaluation.nf new file mode 100644 index 00000000..f0307484 --- /dev/null +++ b/subworkflows/local/variant_evaluation.nf @@ -0,0 +1,49 @@ +// +// A subworkflow to evaluate variants using truth vcfs. +// + +include { RTGTOOLS_VCFEVAL } from '../../modules/nf-core/rtgtools/vcfeval/main' +include { BCFTOOLS_REHEADER } from '../../modules/nf-core/bcftools/reheader/main' +include { TABIX_TABIX as TABIX_TRUTHVCF } from '../../modules/nf-core/tabix/tabix/main' + +workflow VARIANT_EVALUATION { + + take: + ch_snv_vcf_tbi // channel: [mandatory] [ val(meta), path(vcf) ] + ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_rtg_truthvcfs // channel: [mandatory] [ val(meta), path(dbs) ] + + main: + ch_versions = Channel.empty() + + ch_rtg_truthvcfs + .splitCsv ( header:true ) + .map { row -> + return [[id:row.samplename[0], bed:row.bed[0]], row.vcf[0], []] + } + .set { ch_rtgvcfs_dbs } + + BCFTOOLS_REHEADER (ch_rtgvcfs_dbs, [[:],[]]) + + TABIX_TRUTHVCF (BCFTOOLS_REHEADER.out.vcf) + + BCFTOOLS_REHEADER.out.vcf + .join(TABIX_TRUTHVCF.out.tbi) + .map { meta, vcf, tbi -> return [vcf, tbi, meta.bed]} + .set { ch_truthvcf_tbi } + + ch_snv_vcf_tbi + .combine(ch_truthvcf_tbi) + .map { meta, query, qidx, truth, tidx, tbed -> + return [meta, query, qidx, truth, tidx, tbed, []] + } + .set { ch_vcfeval_in } + + RTGTOOLS_VCFEVAL ( ch_vcfeval_in ) + + // ch_versions = ch_versions.mix(BUILD_BED.out.versions) + // ch_versions = ch_versions.mix(GATK4_SPLITINTERVALS.out.versions) + + emit: + versions = ch_versions // channel: [ path(versions.yml) ] +} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 9481b930..63205007 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -34,6 +34,10 @@ def mandatoryParams = [ ] def missingParamsCount = 0 +if (params.run_rtgvcfeval) { + mandatoryParams += ["rtg_truthvcfs"] +} + if (!params.skip_snv_annotation) { mandatoryParams += ["genome", "vcfanno_resources", "vcfanno_toml", "vep_cache", "vep_cache_version", "gnomad_af", "score_config_snv"] @@ -126,6 +130,7 @@ include { ANNOTATE_GENOME_SNVS } from '../subworkflows/local/an include { ANNOTATE_MOBILE_ELEMENTS } from '../subworkflows/local/annotate_mobile_elements' include { ANNOTATE_MT_SNVS } from '../subworkflows/local/annotate_mt_snvs' include { ANNOTATE_STRUCTURAL_VARIANTS } from '../subworkflows/local/annotate_structural_variants' +include { CALL_MOBILE_ELEMENTS } from '../subworkflows/local/call_mobile_elements' include { CALL_REPEAT_EXPANSIONS } from '../subworkflows/local/call_repeat_expansions' include { CALL_SNV } from '../subworkflows/local/call_snv' include { CALL_STRUCTURAL_VARIANTS } from '../subworkflows/local/call_structural_variants' @@ -138,8 +143,7 @@ include { RANK_VARIANTS as RANK_VARIANTS_MT } from '../subworkflows/local/ra include { RANK_VARIANTS as RANK_VARIANTS_SNV } from '../subworkflows/local/rank_variants' include { RANK_VARIANTS as RANK_VARIANTS_SV } from '../subworkflows/local/rank_variants' include { SCATTER_GENOME } from '../subworkflows/local/scatter_genome' -include { CALL_MOBILE_ELEMENTS } from '../subworkflows/local/call_mobile_elements' - +include { VARIANT_EVALUATION } from '../subworkflows/local/variant_evaluation' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -268,6 +272,8 @@ workflow RAREDISEASE { : ( ch_references.readcount_intervals ?: Channel.empty() ) ch_reduced_penetrance = params.reduced_penetrance ? Channel.fromPath(params.reduced_penetrance).collect() : Channel.value([]) + ch_rtg_truthvcfs = params.rtg_truthvcfs ? Channel.fromPath(params.rtg_truthvcfs).collect() + : Channel.value([]) ch_score_config_mt = params.score_config_mt ? Channel.fromPath(params.score_config_mt).collect() : Channel.value([]) ch_score_config_snv = params.score_config_snv ? Channel.fromPath(params.score_config_snv).collect() @@ -397,6 +403,18 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(CALL_SNV.out.versions) + // + // VARIANT EVALUATION + // + CALL_SNV.out.genome_vcf_tabix.view() + if (params.run_rtgvcfeval) { + VARIANT_EVALUATION ( + CALL_SNV.out.genome_vcf_tabix, + ch_genome_fai, + ch_rtg_truthvcfs + ) + } + // // SV CALLING // From 2559c22eb955f3d71485e0c7c5ca2c91f11279f4 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 21 Jan 2024 13:54:19 +0100 Subject: [PATCH 257/387] add format --- modules.json | 5 ++ .../nf-core/rtgtools/format/environment.yml | 7 ++ modules/nf-core/rtgtools/format/main.nf | 66 +++++++++++++++++++ modules/nf-core/rtgtools/format/meta.yml | 51 ++++++++++++++ 4 files changed, 129 insertions(+) create mode 100644 modules/nf-core/rtgtools/format/environment.yml create mode 100644 modules/nf-core/rtgtools/format/main.nf create mode 100644 modules/nf-core/rtgtools/format/meta.yml diff --git a/modules.json b/modules.json index 1c7da4d9..0231c254 100644 --- a/modules.json +++ b/modules.json @@ -347,6 +347,11 @@ "git_sha": "d73505dd68b27b53b4002e84eea21a2819907562", "installed_by": ["modules"] }, + "rtgtools/format": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, "rtgtools/vcfeval": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", diff --git a/modules/nf-core/rtgtools/format/environment.yml b/modules/nf-core/rtgtools/format/environment.yml new file mode 100644 index 00000000..15b3df5b --- /dev/null +++ b/modules/nf-core/rtgtools/format/environment.yml @@ -0,0 +1,7 @@ +name: rtgtools_format +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::rtg-tools=3.12.1 diff --git a/modules/nf-core/rtgtools/format/main.nf b/modules/nf-core/rtgtools/format/main.nf new file mode 100644 index 00000000..802d3b20 --- /dev/null +++ b/modules/nf-core/rtgtools/format/main.nf @@ -0,0 +1,66 @@ +process RTGTOOLS_FORMAT { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/rtg-tools:3.12.1--hdfd78af_0': + 'biocontainers/rtg-tools:3.12.1--hdfd78af_0' }" + + input: + tuple val(meta), path(input1), path(input2), path(sam_rg) + + output: + tuple val(meta), path("*.sdf"), emit: sdf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def single = meta.containsKey("single_end") ? meta.single_end : true + + def input = single ? "${input1}" : "--left ${input1} --right ${input2}" + def rg = sam_rg ? "--sam-rg ${sam_rg}" : "" + + def avail_mem = "3G" + if (!task.memory) { + log.info '[RTG format] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = (task.memory.mega*0.8).intValue() + "M" + } + + """ + rtg RTG_MEM=${avail_mem} format \\ + ${args} \\ + ${rg} \\ + --output ${prefix}.sdf \\ + ${input} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + rtg-tools: \$(echo \$(rtg version | head -n 1 | awk '{print \$4}')) + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + def avail_mem = "3G" + if (!task.memory) { + log.info '[RTG format] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = (task.memory.mega*0.8).intValue() + "M" + } + """ + touch ${prefix}.sdf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + rtg-tools: \$(echo \$(rtg version | head -n 1 | awk '{print \$4}')) + END_VERSIONS + """ +} diff --git a/modules/nf-core/rtgtools/format/meta.yml b/modules/nf-core/rtgtools/format/meta.yml new file mode 100644 index 00000000..8ed18f54 --- /dev/null +++ b/modules/nf-core/rtgtools/format/meta.yml @@ -0,0 +1,51 @@ +name: "rtgtools_format" +description: Converts the contents of sequence data files (FASTA/FASTQ/SAM/BAM) into the RTG Sequence Data File (SDF) format. +keywords: + - rtg + - fasta + - fastq + - bam + - sam +tools: + - "rtgtools": + description: "RealTimeGenomics Tools -- Utilities for accurate VCF comparison and manipulation" + homepage: "https://www.realtimegenomics.com/products/rtg-tools" + documentation: "https://github.com/RealTimeGenomics/rtg-tools" + tool_dev_url: "https://github.com/RealTimeGenomics/rtg-tools" + licence: "['BSD']" +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input1: + type: file + description: FASTA, FASTQ, BAM or SAM file. This should be the left input file when using paired end FASTQ/FASTA data + pattern: "*.{fasta,fa,fna,fastq,fastq.gz,fq,fq.gz,bam,sam}" + - input2: + type: file + description: The right input file when using paired end FASTQ/FASTA data + pattern: "*.{fasta,fa,fna,fastq,fastq.gz,fq,fq.gz}" + - sam_rg: + type: file + description: A file containing a single readgroup header as a SAM header. This can also be supplied as a string in `task.ext.args` as `--sam-rg `. + pattern: "*.{txt,sam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - sdf: + type: directory + description: The sequence dictionary format folder created from the input file(s) + pattern: "*.sdf" +authors: + - "@nvnieuwk" +maintainers: + - "@nvnieuwk" From 681885ddcbfac349764f615c1cd6fc4ccbcb8b74 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 21 Jan 2024 16:53:01 +0100 Subject: [PATCH 258/387] rtg format --- conf/modules/prepare_references.config | 4 ++++ conf/modules/variant_evaluation.config | 18 +++++++++++++++--- subworkflows/local/prepare_references.nf | 6 +++++- subworkflows/local/variant_evaluation.nf | 12 ++++++------ workflows/raredisease.nf | 8 ++++---- 5 files changed, 34 insertions(+), 14 deletions(-) diff --git a/conf/modules/prepare_references.config b/conf/modules/prepare_references.config index 39a0a807..3721d220 100644 --- a/conf/modules/prepare_references.config +++ b/conf/modules/prepare_references.config @@ -53,6 +53,10 @@ process { ext.when = {!params.fai} } + withName: '.*PREPARE_REFERENCES:RTGTOOLS_FORMAT' { + ext.when = { !params.sdf && params.run_rtgvcfeval } + } + withName: '.*PREPARE_REFERENCES:SAMTOOLS_EXTRACT_MT' { ext.args = { " ${params.mito_name} -o ${meta.id}_mt.fa" } ext.when = {!params.mt_fasta} diff --git a/conf/modules/variant_evaluation.config b/conf/modules/variant_evaluation.config index 04ea6baa..4059b3a7 100644 --- a/conf/modules/variant_evaluation.config +++ b/conf/modules/variant_evaluation.config @@ -21,10 +21,22 @@ process { } withName: '.*VARIANT_EVALUATION:BCFTOOLS_REHEADER' { - beforeScript = { "echo ${meta.id} > ${meta.id}.txt" } - ext.args = { "--samples ${meta.id}.txt" } + beforeScript = { "echo ${meta.samplename} > ${meta.samplename}.txt" } + ext.args = { "--samples ${meta.samplename}.txt" } ext.args2 = "--output-type z" ext.when = { params.run_rtgvcfeval } - ext.prefix = { "${meta.id}_truthvcf" } + ext.prefix = { "${meta.samplename}_truthvcf" } } + + withName: '.*VARIANT_EVALUATION:RTGTOOLS_VCFEVAL' { + ext.args = { "--sample ${meta.samplename} --output-mode=split" } + ext.when = { params.run_rtgvcfeval } + ext.prefix = { "${meta.samplename}_vcfeval" } + publishDir = [ + path: { "${params.outdir}/rtgvcfeval" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + } diff --git a/subworkflows/local/prepare_references.nf b/subworkflows/local/prepare_references.nf index bacde149..b98b89d1 100644 --- a/subworkflows/local/prepare_references.nf +++ b/subworkflows/local/prepare_references.nf @@ -15,6 +15,7 @@ include { GATK4_PREPROCESSINTERVALS as GATK_PREPROCESS_WGS } from '../../modul include { GATK4_PREPROCESSINTERVALS as GATK_PREPROCESS_WES } from '../../modules/nf-core/gatk4/preprocessintervals/main.nf' include { GATK4_SHIFTFASTA as GATK_SHIFTFASTA } from '../../modules/nf-core/gatk4/shiftfasta/main' include { GET_CHROM_SIZES } from '../../modules/local/get_chrom_sizes' +include { RTGTOOLS_FORMAT } from '../../modules/nf-core/rtgtools/format/main' include { SAMTOOLS_FAIDX as SAMTOOLS_EXTRACT_MT } from '../../modules/nf-core/samtools/faidx/main' include { SAMTOOLS_FAIDX as SAMTOOLS_FAIDX_GENOME } from '../../modules/nf-core/samtools/faidx/main' include { SAMTOOLS_FAIDX as SAMTOOLS_FAIDX_MT_SHIFT } from '../../modules/nf-core/samtools/faidx/main' @@ -51,6 +52,8 @@ workflow PREPARE_REFERENCES { GATK_SD(ch_genome_fasta) ch_fai = Channel.empty().mix(ch_genome_fai, SAMTOOLS_FAIDX_GENOME.out.fai).collect() GET_CHROM_SIZES( ch_fai ) + ch_genome_fasta.map {meta, fasta -> return [meta, fasta, [], [] ]}.set {ch_rtgformat_in} + RTGTOOLS_FORMAT(ch_rtgformat_in) // MT indices SAMTOOLS_EXTRACT_MT(ch_genome_fasta, ch_fai) @@ -120,6 +123,7 @@ workflow PREPARE_REFERENCES { ch_versions = ch_versions.mix(UNTAR_VEP_CACHE.out.versions) ch_versions = ch_versions.mix(GATK_PREPROCESS_WGS.out.versions) ch_versions = ch_versions.mix(GATK_PREPROCESS_WES.out.versions) + ch_versions = ch_versions.mix(RTGTOOLS_FORMAT.out.versions) emit: genome_bwa_index = Channel.empty().mix(ch_bwa, ch_sentieonbwa).collect() // channel: [ val(meta), path(index) ] @@ -129,7 +133,7 @@ workflow PREPARE_REFERENCES { genome_dict = GATK_SD.out.dict.collect() // channel: [ path(dict) ] readcount_intervals = Channel.empty() .mix(ch_preprocwgs.interval_list,ch_preprocwes.interval_list)// channel: [ path(intervals) ] - + sdf = RTGTOOLS_FORMAT.out.sdf // channel: [ val (meta), path(intervals) ] mt_intervals = ch_shiftfasta_mtintervals.intervals.collect() // channel: [ path(intervals) ] mtshift_intervals = ch_shiftfasta_mtintervals.shift_intervals.collect() // channel: [ path(intervals) ] mtshift_backchain = GATK_SHIFTFASTA.out.shift_back_chain.collect() // channel: [ val(meta), path(backchain) ] diff --git a/subworkflows/local/variant_evaluation.nf b/subworkflows/local/variant_evaluation.nf index f0307484..f92dcd20 100644 --- a/subworkflows/local/variant_evaluation.nf +++ b/subworkflows/local/variant_evaluation.nf @@ -9,9 +9,10 @@ include { TABIX_TABIX as TABIX_TRUTHVCF } from '../../modules/nf-core/tabix/t workflow VARIANT_EVALUATION { take: - ch_snv_vcf_tbi // channel: [mandatory] [ val(meta), path(vcf) ] + ch_snv_vcf_tbi // channel: [mandatory] [ val(meta), path(vcf), path(tbi) ] ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] ch_rtg_truthvcfs // channel: [mandatory] [ val(meta), path(dbs) ] + ch_sdf // channel: [mandatory] [ val(meta), path(sdf) ] main: ch_versions = Channel.empty() @@ -19,7 +20,7 @@ workflow VARIANT_EVALUATION { ch_rtg_truthvcfs .splitCsv ( header:true ) .map { row -> - return [[id:row.samplename[0], bed:row.bed[0]], row.vcf[0], []] + return [[samplename:row.samplename[0], bed:row.bed[0]], row.vcf[0], []] } .set { ch_rtgvcfs_dbs } @@ -29,17 +30,16 @@ workflow VARIANT_EVALUATION { BCFTOOLS_REHEADER.out.vcf .join(TABIX_TRUTHVCF.out.tbi) - .map { meta, vcf, tbi -> return [vcf, tbi, meta.bed]} .set { ch_truthvcf_tbi } ch_snv_vcf_tbi .combine(ch_truthvcf_tbi) - .map { meta, query, qidx, truth, tidx, tbed -> - return [meta, query, qidx, truth, tidx, tbed, []] + .map { meta, query, qidx, meta2, truth, tidx -> + return [meta + [samplename: meta2.samplename] , query, qidx, truth, tidx, meta2.bed, []] } .set { ch_vcfeval_in } - RTGTOOLS_VCFEVAL ( ch_vcfeval_in ) + RTGTOOLS_VCFEVAL ( ch_vcfeval_in, ch_sdf ) // ch_versions = ch_versions.mix(BUILD_BED.out.versions) // ch_versions = ch_versions.mix(GATK4_SPLITINTERVALS.out.versions) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 63205007..8ddccb1f 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -254,7 +254,7 @@ workflow RAREDISEASE { : Channel.empty() ch_me_references = params.mobile_element_references ? Channel.fromSamplesheet("mobile_element_references") : Channel.empty() - ch_me_svdb_resources = params.mobile_element_svdb_annotations ? Channel.fromPath(params.mobile_element_svdb_annotations) + ch_me_svdb_resources = params.mobile_element_svdb_annotations ? Channel.fromPath(params.mobile_element_svdb_annotations) : Channel.empty() ch_ml_model = params.variant_caller.equals("sentieon") ? Channel.fromPath(params.ml_model).map {it -> [[id:it[0].simpleName], it]}.collect() : Channel.value([[:],[]]) @@ -280,6 +280,7 @@ workflow RAREDISEASE { : Channel.value([]) ch_score_config_sv = params.score_config_sv ? Channel.fromPath(params.score_config_sv).collect() : Channel.value([]) + ch_sdf = ch_references.sdf ch_sv_dbs = params.svdb_query_dbs ? Channel.fromPath(params.svdb_query_dbs) : Channel.empty() ch_sv_bedpedbs = params.svdb_query_bedpedbs ? Channel.fromPath(params.svdb_query_bedpedbs) @@ -303,7 +304,6 @@ workflow RAREDISEASE { : Channel.value([]) ch_versions = ch_versions.mix(ch_references.versions) - // SV caller priority if (params.skip_germlinecnvcaller) { ch_svcaller_priority = Channel.value(["tiddit", "manta", "cnvnator"]) @@ -406,12 +406,12 @@ workflow RAREDISEASE { // // VARIANT EVALUATION // - CALL_SNV.out.genome_vcf_tabix.view() if (params.run_rtgvcfeval) { VARIANT_EVALUATION ( CALL_SNV.out.genome_vcf_tabix, ch_genome_fai, - ch_rtg_truthvcfs + ch_rtg_truthvcfs, + ch_sdf ) } From e64f431425c869ef1441dd98b195942c10887c5e Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 21 Jan 2024 17:35:19 +0100 Subject: [PATCH 259/387] add sdf --- assets/rtg_truthvcfs.json | 4 ++-- main.nf | 1 + nextflow_schema.json | 8 ++++++++ subworkflows/local/variant_evaluation.nf | 2 +- 4 files changed, 12 insertions(+), 3 deletions(-) diff --git a/assets/rtg_truthvcfs.json b/assets/rtg_truthvcfs.json index 35e1228e..3c3fe1db 100644 --- a/assets/rtg_truthvcfs.json +++ b/assets/rtg_truthvcfs.json @@ -19,7 +19,7 @@ "pattern": "^\\S+\\.vcf(\\.gz)?$", "errorMessage": "Path to vcf files cannot contain spaces and must be a .vcf or .vcf.gz file" }, - "bed": { + "bedregions": { "type": "string", "format": "file-path", "exists": true, @@ -27,6 +27,6 @@ "errorMessage": "Path to bed files cannot contain spaces" } }, - "required": ["vcf", "samplename", "bed"] + "required": ["vcf", "samplename", "bedregions"] } } diff --git a/main.nf b/main.nf index ef782474..aebcea25 100644 --- a/main.nf +++ b/main.nf @@ -44,6 +44,7 @@ params.sequence_dictionary = WorkflowMain.getGenomeAttribute(params, params.score_config_mt = WorkflowMain.getGenomeAttribute(params, 'score_config_mt') params.score_config_snv = WorkflowMain.getGenomeAttribute(params, 'score_config_snv') params.score_config_sv = WorkflowMain.getGenomeAttribute(params, 'score_config_sv') +params.sdf = WorkflowMain.getGenomeAttribute(params, 'sdf') params.svdb_query_dbs = WorkflowMain.getGenomeAttribute(params, 'svdb_query_dbs') params.target_bed = WorkflowMain.getGenomeAttribute(params, 'target_bed') params.min_trimmed_length = WorkflowMain.getGenomeAttribute(params, 'min_trimmed_length') diff --git a/nextflow_schema.json b/nextflow_schema.json index 68558759..5c77956e 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -309,6 +309,14 @@ "fa_icon": "fas fa-file", "description": "SV rank model config file for genmod." }, + "sdf": { + "type": "string", + "exists": true, + "format": "directory-path", + "description": "Directory for pre-built sdf index. Used by rtg/vcfeval", + "help_text": "If none provided, will be generated automatically from the FASTA reference.", + "fa_icon": "fas fa-folder-open" + }, "sequence_dictionary": { "type": "string", "exists": true, diff --git a/subworkflows/local/variant_evaluation.nf b/subworkflows/local/variant_evaluation.nf index f92dcd20..8d550cdf 100644 --- a/subworkflows/local/variant_evaluation.nf +++ b/subworkflows/local/variant_evaluation.nf @@ -20,7 +20,7 @@ workflow VARIANT_EVALUATION { ch_rtg_truthvcfs .splitCsv ( header:true ) .map { row -> - return [[samplename:row.samplename[0], bed:row.bed[0]], row.vcf[0], []] + return [[samplename:row.samplename[0], bedregions:row.bedregions[0]], row.vcf[0], []] } .set { ch_rtgvcfs_dbs } From 4c6851e0b85cf9ee58e43a1767f47a935da4d243 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 22 Jan 2024 10:01:31 +0100 Subject: [PATCH 260/387] update module --- modules.json | 2 +- modules/nf-core/ensemblvep/vep/main.nf | 5 +- .../nf-core/ensemblvep/vep/tests/main.nf.test | 102 ++++++++++++++++++ .../ensemblvep/vep/tests/nextflow.config | 13 +++ .../ensemblvep/vep/tests/tab.gz.config | 5 + modules/nf-core/ensemblvep/vep/tests/tags.yml | 2 + .../nf-core/ensemblvep/vep/tests/vcf.config | 5 + 7 files changed, 130 insertions(+), 4 deletions(-) create mode 100644 modules/nf-core/ensemblvep/vep/tests/main.nf.test create mode 100644 modules/nf-core/ensemblvep/vep/tests/nextflow.config create mode 100644 modules/nf-core/ensemblvep/vep/tests/tab.gz.config create mode 100644 modules/nf-core/ensemblvep/vep/tests/tags.yml create mode 100644 modules/nf-core/ensemblvep/vep/tests/vcf.config diff --git a/modules.json b/modules.json index eac809df..03000a35 100644 --- a/modules.json +++ b/modules.json @@ -117,7 +117,7 @@ }, "ensemblvep/vep": { "branch": "master", - "git_sha": "214d575774c172062924ad3564b4f66655600730", + "git_sha": "76a0696a60c41c57fc5f6040ac31b11ce5d4d8dd", "installed_by": ["modules"] }, "expansionhunter": { diff --git a/modules/nf-core/ensemblvep/vep/main.nf b/modules/nf-core/ensemblvep/vep/main.nf index 3a2b7423..a7fc5ad1 100644 --- a/modules/nf-core/ensemblvep/vep/main.nf +++ b/modules/nf-core/ensemblvep/vep/main.nf @@ -20,7 +20,7 @@ process ENSEMBLVEP_VEP { tuple val(meta), path("*.vcf.gz") , optional:true, emit: vcf tuple val(meta), path("*.tab.gz") , optional:true, emit: tab tuple val(meta), path("*.json.gz") , optional:true, emit: json - path "*.summary.html" , emit: report + path "*.summary.html" , optional:true, emit: report path "versions.yml" , emit: versions when: @@ -45,8 +45,7 @@ process ENSEMBLVEP_VEP { --cache \\ --cache_version $cache_version \\ --dir_cache $dir_cache \\ - --fork $task.cpus \\ - --stats_file ${prefix}.summary.html \\ + --fork $task.cpus cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/ensemblvep/vep/tests/main.nf.test b/modules/nf-core/ensemblvep/vep/tests/main.nf.test new file mode 100644 index 00000000..f072dcab --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/tests/main.nf.test @@ -0,0 +1,102 @@ +nextflow_process { + + name "Test Process ENSEMBLVEP_VEP" + script "modules/nf-core/ensemblvep/vep/main.nf" + process "ENSEMBLVEP_VEP" + config "./nextflow.config" + tag "modules" + tag "modules_nfcore" + tag "ensemblvep" + tag "ensemblvep/vep" + tag "ensemblvep/download" + + + test("test_ensemblvep_vep_fasta_vcf") { + + config "./vcf.config" + + setup { + run("ENSEMBLVEP_DOWNLOAD") { + script "../../download/main.nf" + process { + """ + input[0] = Channel.of([[id:"${params.vep_cache_version}_${params.vep_genome}"], params.vep_genome, params.vep_species, params.vep_cache_version]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true), + [] + ]) + input[1] = params.vep_genome + input[2] = params.vep_species + input[3] = params.vep_cache_version + input[4] = ENSEMBLVEP_DOWNLOAD.out.cache.map{ meta, cache -> [cache] } + input[5] = Channel.value([ + [id:"fasta"], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ]) + input[6] = [] + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert path(process.out.vcf.get(0).get(1)).linesGzip.contains("##fileformat=VCFv4.2")} + ) + } + + } + + test("test_ensemblvep_vep_fasta_tab_gz") { + + config "./tab.gz.config" + + setup { + run("ENSEMBLVEP_DOWNLOAD") { + script "../../download/main.nf" + process { + """ + input[0] = Channel.of([[id:"${params.vep_cache_version}_${params.vep_genome}"], params.vep_genome, params.vep_species, params.vep_cache_version]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true), + [] + ]) + input[1] = params.vep_genome + input[2] = params.vep_species + input[3] = params.vep_cache_version + input[4] = ENSEMBLVEP_DOWNLOAD.out.cache.map{ meta, cache -> [cache] } + input[5] = Channel.value([ + [id:"fasta"], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ]) + input[6] = [] + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert path(process.out.tab.get(0).get(1)).linesGzip.contains("## ENSEMBL VARIANT EFFECT PREDICTOR v110.0")} + ) + } + } +} diff --git a/modules/nf-core/ensemblvep/vep/tests/nextflow.config b/modules/nf-core/ensemblvep/vep/tests/nextflow.config new file mode 100644 index 00000000..cfaef733 --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/tests/nextflow.config @@ -0,0 +1,13 @@ +params { + vep_cache_version = "110" + vep_genome = "WBcel235" + vep_species = "caenorhabditis_elegans" +} + +process { + + withName: ENSEMBLVEP_DOWNLOAD { + ext.args = '--AUTO c --CONVERT --NO_BIOPERL --NO_HTSLIB --NO_TEST --NO_UPDATE' + } + +} diff --git a/modules/nf-core/ensemblvep/vep/tests/tab.gz.config b/modules/nf-core/ensemblvep/vep/tests/tab.gz.config new file mode 100644 index 00000000..40eb03e5 --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/tests/tab.gz.config @@ -0,0 +1,5 @@ +process { + withName: ENSEMBLVEP_VEP { + ext.args = '--tab --compress_output bgzip' + } +} diff --git a/modules/nf-core/ensemblvep/vep/tests/tags.yml b/modules/nf-core/ensemblvep/vep/tests/tags.yml new file mode 100644 index 00000000..4aa4aa45 --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/tests/tags.yml @@ -0,0 +1,2 @@ +ensemblvep/vep: + - "modules/nf-core/ensemblvep/vep/**" diff --git a/modules/nf-core/ensemblvep/vep/tests/vcf.config b/modules/nf-core/ensemblvep/vep/tests/vcf.config new file mode 100644 index 00000000..ad8955a3 --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/tests/vcf.config @@ -0,0 +1,5 @@ +process { + withName: ENSEMBLVEP_VEP { + ext.args = '--vcf' + } +} From ab36f9bb30c31095924129413186fedafb8b1958 Mon Sep 17 00:00:00 2001 From: jemten Date: Mon, 22 Jan 2024 11:49:48 +0100 Subject: [PATCH 261/387] refactoring clinical research split --- conf/modules/annotate_consequence_pli.config | 33 ++---- conf/modules/annotate_mobile_elements.config | 7 +- conf/modules/generate_clinical_set.config | 76 ++++++++++++ conf/modules/rank_variants.config | 48 +++++--- conf/modules/raredisease.config | 62 ---------- nextflow.config | 1 + .../local/annotate_consequence_pli.nf | 4 +- .../local/annotate_mobile_elements.nf | 44 +++---- subworkflows/local/generate_clinical_set.nf | 45 ++++++++ workflows/raredisease.nf | 108 ++++++++---------- 10 files changed, 234 insertions(+), 194 deletions(-) create mode 100644 conf/modules/generate_clinical_set.config create mode 100644 subworkflows/local/generate_clinical_set.nf diff --git a/conf/modules/annotate_consequence_pli.config b/conf/modules/annotate_consequence_pli.config index 7fde1038..e9451357 100644 --- a/conf/modules/annotate_consequence_pli.config +++ b/conf/modules/annotate_consequence_pli.config @@ -21,20 +21,15 @@ process { } withName: '.*ANN_CSQ_PLI_SV:ADD_MOST_SEVERE_CSQ' { - ext.prefix = { "${meta.id}_genome_vep_csq" } + ext.prefix = { "${meta.id}_sv_csq_${meta.set}" } } withName: '.*ANN_CSQ_PLI_SV:ADD_MOST_SEVERE_PLI' { - ext.prefix = { "${meta.id}_genome_vep_csq_pli" } + ext.prefix = { "${meta.id}_sv_csq_pli_${meta.set}" } } withName: '.*ANN_CSQ_PLI_SV:TABIX_BGZIPTABIX' { - ext.prefix = { "${meta.id}_genome_vep_csq_pli" } - publishDir = [ - path: { "${params.outdir}/annotate_sv" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + ext.prefix = { "${meta.id}_sv_csq_pli_${meta.set}" } } } } @@ -49,20 +44,15 @@ process { } withName: '.*ANN_CSQ_PLI_SNV:ADD_MOST_SEVERE_CSQ' { - ext.prefix = { "${meta.id}_vep_csq" } + ext.prefix = { "${meta.id}_snv_csq_${meta.set}" } } withName: '.*ANN_CSQ_PLI_SNV:ADD_MOST_SEVERE_PLI' { - ext.prefix = { "${meta.id}_vep_csq_pli" } + ext.prefix = { "${meta.id}_snv_csq_pli_${meta.set}" } } withName: '.*ANN_CSQ_PLI_SNV:TABIX_BGZIPTABIX' { - ext.prefix = { "${meta.id}_vep_csq_pli" } - publishDir = [ - path: { "${params.outdir}/annotate_snv/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + ext.prefix = { "${meta.id}_snv_csq_pli_${meta.set}" } } } } @@ -77,20 +67,15 @@ process { } withName: '.*ANN_CSQ_PLI_MT:ADD_MOST_SEVERE_CSQ' { - ext.prefix = { "${meta.id}_mt_vep_csq" } + ext.prefix = { "${meta.id}_mt_csq_${meta.set}" } } withName: '.*ANN_CSQ_PLI_MT:ADD_MOST_SEVERE_PLI' { - ext.prefix = { "${meta.id}_mt_vep_csq_pli" } + ext.prefix = { "${meta.id}_mt_csq_pli_${meta.set}" } } withName: '.*ANN_CSQ_PLI_MT:TABIX_BGZIPTABIX' { - ext.prefix = { "${meta.id}_mt_vep_csq_pli" } - publishDir = [ - path: { "${params.outdir}/annotate_snv/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] + ext.prefix = { "${meta.id}_mt_csq_pli_${meta.set}" } } } } diff --git a/conf/modules/annotate_mobile_elements.config b/conf/modules/annotate_mobile_elements.config index 0e04095c..14992db2 100644 --- a/conf/modules/annotate_mobile_elements.config +++ b/conf/modules/annotate_mobile_elements.config @@ -58,12 +58,17 @@ process { ext.prefix = { "${meta.id}_filter" } } - withName: '.*ANNOTATE_MOBILE_ELEMENTS:FILTERVEP_ME' { + withName: '.*:ANNOTATE_MOBILE_ELEMENTS:GENERATE_CLINICAL_SET_ME:ENSEMBLVEP_FILTERVEP' { ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_me_${meta.set}" } ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } } + withName: '.*:ANNOTATE_MOBILE_ELEMENTS:GENERATE_CLINICAL_SET_ME:TABIX_BGZIP' { + ext.when = !params.skip_vep_filter + ext.prefix = { "${meta.id}_me_${meta.set}" } + } + withName: '.*ANNOTATE_MOBILE_ELEMENTS:ANNOTATE_CSQ_PLI_ME:ADD_MOST_SEVERE_CSQ' { ext.prefix = { "${meta.id}_me_csq_${meta.set}" } } diff --git a/conf/modules/generate_clinical_set.config b/conf/modules/generate_clinical_set.config new file mode 100644 index 00000000..8de8d594 --- /dev/null +++ b/conf/modules/generate_clinical_set.config @@ -0,0 +1,76 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. + ext.when = Conditional clause +---------------------------------------------------------------------------------------- +*/ + +// +// Filter out clinical and research variants +// + +// SNVs + +process { + withName: '.*:GENERATE_CLINICAL_SET_SNV:ENSEMBLVEP_FILTERVEP' { + ext.when = !params.skip_vep_filter + ext.prefix = { "${meta.id}_snv_${meta.set}" } + ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } + } + + withName: '.*:GENERATE_CLINICAL_SET_SNV:TABIX_BGZIP' { + ext.when = !params.skip_vep_filter + ext.prefix = { "${meta.id}_snv_${meta.set}" } + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } +} + +// SVs + +process { + withName: '.*:GENERATE_CLINICAL_SET_SV:ENSEMBLVEP_FILTERVEP' { + ext.when = !params.skip_vep_filter + ext.prefix = { "${meta.id}_sv_${meta.set}" } + ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } + } + + withName: '.*:GENERATE_CLINICAL_SET_SV:TABIX_BGZIP' { + ext.when = !params.skip_vep_filter + ext.prefix = { "${meta.id}_sv_${meta.set}" } + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } +} + +// MT variants + +process { + withName: '.*:GENERATE_CLINICAL_SET_MT:ENSEMBLVEP_FILTERVEP' { + ext.when = !params.skip_vep_filter + ext.prefix = { "${meta.id}_mt_${meta.set}" } + ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } + } + + withName: '.*:GENERATE_CLINICAL_SET_MT:TABIX_BGZIP' { + ext.when = !params.skip_vep_filter + ext.prefix = { "${meta.id}_mt_${meta.set}" } + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } +} diff --git a/conf/modules/rank_variants.config b/conf/modules/rank_variants.config index 65da5cc3..b65d55ab 100644 --- a/conf/modules/rank_variants.config +++ b/conf/modules/rank_variants.config @@ -24,22 +24,30 @@ process { } withName: '.*RANK_VARIANTS_SV:GENMOD_ANNOTATE' { - ext.args = { - (params.genome == 'GRCh37') ? '--annotate_regions --genome-build 37' : '--annotate_regions --genome-build 38' - } + ext.prefix = { "${meta.id}_sv_genmod_annotate_${meta.set}" } + ext.args = { [ + '--annotate_regions', + params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38' + ].join(' ') } + } + + withName: '.*RANK_VARIANTS_SNV:GENMOD_MODELS' { + ext.prefix = { "${meta.id}_snv_genmod_models_${meta.set}" } + ext.args = " --whole_gene " } withName: '.*RANK_VARIANTS_SV:GENMOD_SCORE' { + ext.prefix = { "${meta.id}_sv_genmod_score_${meta.set}" } ext.args = " --rank_results " } withName: '.*RANK_VARIANTS_SV:GENMOD_COMPOUND' { - ext.prefix = { "${meta.id}_ranked_sv" } + ext.prefix = { "${meta.id}_sv_genmod_compound_${meta.set}" } } withName: '.*RANK_VARIANTS_SV:BCFTOOLS_SORT' { ext.args = "--output-type z" - ext.prefix = { "${meta.id}_ranked_sv" } + ext.prefix = { "${meta.id}_sv_ranked_${meta.set}" } publishDir = [ path: { "${params.outdir}/rank_and_filter" }, mode: params.publish_dir_mode, @@ -75,21 +83,25 @@ process { } withName: '.*RANK_VARIANTS_SNV:GENMOD_ANNOTATE' { - ext.args = { - (params.genome == 'GRCh37') ? '--annotate_regions --genome-build 37' : '--annotate_regions --genome-build 38' - } + ext.prefix = { "${meta.id}_snv_genmod_annotate_${meta.set}" } + ext.args = { [ + '--annotate_regions', + params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38' + ].join(' ') } } withName: '.*RANK_VARIANTS_SNV:GENMOD_MODELS' { + ext.prefix = { "${meta.id}_snv_genmod_models_${meta.set}" } ext.args = " --whole_gene " } withName: '.*RANK_VARIANTS_SNV:GENMOD_SCORE' { + ext.prefix = { "${meta.id}_snv_genmod_score_${meta.set}" } ext.args = " --rank_results " } withName: '.*RANK_VARIANTS_SNV:GENMOD_COMPOUND' { - ext.prefix = { "${meta.id}_ranked_snv" } + ext.prefix = { "${meta.id}_snv_genmod_compound_${meta.set}" } } withName: '.*RANK_VARIANTS_SNV:BCFTOOLS_SORT' { @@ -97,7 +109,7 @@ process { } withName: '.*RANK_VARIANTS_SNV:TABIX_BGZIP' { - ext.prefix = { "${meta.id}_ranked_snv" } + ext.prefix = { "${meta.id}_snv_ranked_${meta.set}" } publishDir = [ path: { "${params.outdir}/rank_and_filter" }, mode: params.publish_dir_mode, @@ -122,28 +134,32 @@ process { process { if (!params.skip_mt_annotation) { withName: '.*RANK_VARIANTS_MT:.*' { - ext.when = !params.skip_snv_annotation + ext.when = !params.skip_mt_annotation publishDir = [ enabled: false ] } withName: '.*RANK_VARIANTS_MT:GENMOD_ANNOTATE' { - ext.args = { - (params.genome == 'GRCh37') ? '--annotate_regions --genome-build 37' : '--annotate_regions --genome-build 38' - } + ext.prefix = { "${meta.id}_mt_genmod_annotate_${meta.set}" } + ext.args = { [ + '--annotate_regions', + params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38' + ].join(' ') } } withName: '.*RANK_VARIANTS_MT:GENMOD_MODELS' { + ext.prefix = { "${meta.id}_mt_genmod_models_${meta.set}" } ext.args = " --whole_gene " } withName: '.*RANK_VARIANTS_MT:GENMOD_SCORE' { + ext.prefix = { "${meta.id}_mt_genmod_score_${meta.set}" } ext.args = " --rank_results " } withName: '.*RANK_VARIANTS_MT:GENMOD_COMPOUND' { - ext.prefix = { "${meta.id}_ranked_mt" } + ext.prefix = { "${meta.id}_mt_genmod_compound_${meta.set}" } } withName: '.*RANK_VARIANTS_MT:BCFTOOLS_SORT' { @@ -151,7 +167,7 @@ process { } withName: '.*RANK_VARIANTS_MT:TABIX_BGZIP' { - ext.prefix = { "${meta.id}_ranked_mt" } + ext.prefix = { "${meta.id}_mt_ranked_${meta.set}" } publishDir = [ path: { "${params.outdir}/rank_and_filter" }, mode: params.publish_dir_mode, diff --git a/conf/modules/raredisease.config b/conf/modules/raredisease.config index 6287348e..2eae1c58 100644 --- a/conf/modules/raredisease.config +++ b/conf/modules/raredisease.config @@ -43,8 +43,6 @@ process { } } - - // // Smncopynumbercaller options // @@ -63,66 +61,6 @@ process { } } -// -// Filter out clincal and research variants -// - -process { - - withName: '.*FILTERVEP_SNV' { - ext.when = !params.skip_vep_filter - ext.prefix = { "${meta.id}_clinical_snv" } - ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } - } - - withName: '.*BGZIPTABIX_SNV' { - ext.when = !params.skip_vep_filter - ext.prefix = { "${meta.id}_clinical_snv" } - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } -} - -process { - - withName: '.*FILTERVEP_SV' { - ext.when = !params.skip_vep_filter - ext.prefix = { "${meta.id}_clinical_sv" } - ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } - } - - withName: '.*BGZIPTABIX_SV' { - ext.when = !params.skip_vep_filter - ext.prefix = { "${meta.id}_clinical_sv" } - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } -} - -process { - withName: '.*FILTERVEP_MT' { - ext.when = !params.skip_vep_filter - ext.prefix = { "${meta.id}_clinical_mt" } - ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } - } - - withName: '.*BGZIPTABIX_MT' { - ext.when = !params.skip_vep_filter - ext.prefix = { "${meta.id}_clinical_mt" } - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } -} - // // Multiqc options // diff --git a/nextflow.config b/nextflow.config index 9634ad31..1348548f 100644 --- a/nextflow.config +++ b/nextflow.config @@ -334,6 +334,7 @@ includeConfig 'conf/modules/call_sv_tiddit.config' includeConfig 'conf/modules/postprocess_MT_calls.config' includeConfig 'conf/modules/call_mobile_elements.config' includeConfig 'conf/modules/annotate_mobile_elements.config' +includeConfig 'conf/modules/generate_clinical_set.config' // Function to ensure that resource requirements don't go beyond // a maximum limit diff --git a/subworkflows/local/annotate_consequence_pli.nf b/subworkflows/local/annotate_consequence_pli.nf index b84d948f..5b625a7b 100644 --- a/subworkflows/local/annotate_consequence_pli.nf +++ b/subworkflows/local/annotate_consequence_pli.nf @@ -25,7 +25,7 @@ workflow ANNOTATE_CSQ_PLI { ch_versions = ch_versions.mix(TABIX_BGZIPTABIX.out.versions) emit: - vcf_ann = TABIX_BGZIPTABIX.out.gz_tbi.map { meta, vcf, tbi -> return [ meta, vcf ] }.collect() // channel: [ val(meta), path(vcf) ] - tbi_ann = TABIX_BGZIPTABIX.out.gz_tbi.map { meta, vcf, tbi -> return [ meta, tbi ] }.collect() // channel: [ val(meta), path(tbi) ] + vcf_ann = TABIX_BGZIPTABIX.out.gz_tbi.map { meta, vcf, tbi -> return [ meta, vcf ] } // channel: [ val(meta), path(vcf) ] + tbi_ann = TABIX_BGZIPTABIX.out.gz_tbi.map { meta, vcf, tbi -> return [ meta, tbi ] } // channel: [ val(meta), path(tbi) ] versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/subworkflows/local/annotate_mobile_elements.nf b/subworkflows/local/annotate_mobile_elements.nf index 04b15ad2..bcc8662c 100644 --- a/subworkflows/local/annotate_mobile_elements.nf +++ b/subworkflows/local/annotate_mobile_elements.nf @@ -2,14 +2,14 @@ // A subworkflow to annotate structural variants. // -include { SVDB_QUERY as SVDB_QUERY_DB } from '../../modules/nf-core/svdb/query/main' -include { PICARD_SORTVCF } from '../../modules/nf-core/picard/sortvcf/main' -include { ENSEMBLVEP as ENSEMBLVEP_ME } from '../../modules/local/ensemblvep/main' -include { ENSEMBLVEP_FILTERVEP as FILTERVEP_ME } from '../../modules/nf-core/ensemblvep/filtervep' include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_FILTER } from '../../modules/nf-core/bcftools/view/main' +include { ENSEMBLVEP as ENSEMBLVEP_ME } from '../../modules/local/ensemblvep/main' +include { PICARD_SORTVCF } from '../../modules/nf-core/picard/sortvcf/main' +include { SVDB_QUERY as SVDB_QUERY_DB } from '../../modules/nf-core/svdb/query/main' include { TABIX_BGZIPTABIX as BGZIP_TABIX_ME } from '../../modules/nf-core/tabix/bgziptabix/main' -include { ANNOTATE_CSQ_PLI as ANNOTATE_CSQ_PLI_ME } from '../../subworkflows/local/annotate_consequence_pli.nf' +include { ANNOTATE_CSQ_PLI as ANNOTATE_CSQ_PLI_ME } from '../../subworkflows/local/annotate_consequence_pli.nf' +include { GENERATE_CLINICAL_SET as GENERATE_CLINICAL_SET_ME } from '../../subworkflows/local/generate_clinical_set.nf' workflow ANNOTATE_MOBILE_ELEMENTS { @@ -71,36 +71,24 @@ workflow ANNOTATE_MOBILE_ELEMENTS { } .set { ch_bcftools_filter_input } - BCFTOOLS_VIEW_FILTER ( ch_bcftools_filter_input, [], [], [] ) + BCFTOOLS_VIEW_FILTER( ch_bcftools_filter_input, [], [], [] ) - BCFTOOLS_VIEW_FILTER.out.vcf - .multiMap { meta, vcf -> - clinical: [ meta + [ set: "clinical" ], vcf ] - research: [ meta + [ set: "research" ], vcf ] - } - .set { ch_clin_research_vcf } - - FILTERVEP_ME( - ch_clin_research_vcf.clinical, + GENERATE_CLINICAL_SET_ME( + BCFTOOLS_VIEW_FILTER.out.vcf, ch_vep_filters ) - ch_clin_research_vcf.research - .mix( FILTERVEP_ME.out.output ) - .set { ch_annotate_csq_pli_me_input } - - - ANNOTATE_CSQ_PLI_ME ( - ch_annotate_csq_pli_me_input, + ANNOTATE_CSQ_PLI_ME( + GENERATE_CLINICAL_SET_ME.out.vcf, ch_variant_consequences ) - ch_versions = ch_versions.mix(SVDB_QUERY_DB.out.versions) - ch_versions = ch_versions.mix(PICARD_SORTVCF.out.versions) - ch_versions = ch_versions.mix(ENSEMBLVEP_ME.out.versions) - ch_versions = ch_versions.mix(BCFTOOLS_VIEW_FILTER.out.versions) - ch_versions = ch_versions.mix(FILTERVEP_ME.out.versions) - ch_versions = ch_versions.mix(ANNOTATE_CSQ_PLI_ME.out.versions) + ch_versions = ch_versions.mix( SVDB_QUERY_DB.out.versions ) + ch_versions = ch_versions.mix( PICARD_SORTVCF.out.versions ) + ch_versions = ch_versions.mix( ENSEMBLVEP_ME.out.versions ) + ch_versions = ch_versions.mix( BCFTOOLS_VIEW_FILTER.out.versions ) + ch_versions = ch_versions.mix( GENERATE_CLINICAL_SET_ME.out.versions ) + ch_versions = ch_versions.mix( ANNOTATE_CSQ_PLI_ME.out.versions ) emit: vcf = ANNOTATE_CSQ_PLI_ME.out.vcf_ann // channel: [ val(meta), path(vcf) ] diff --git a/subworkflows/local/generate_clinical_set.nf b/subworkflows/local/generate_clinical_set.nf new file mode 100644 index 00000000..e877c39b --- /dev/null +++ b/subworkflows/local/generate_clinical_set.nf @@ -0,0 +1,45 @@ +// +// Generarte clinical set of variants +// + +include { ENSEMBLVEP_FILTERVEP } from '../../modules/nf-core/ensemblvep/filtervep' +include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip' +include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix' + +workflow GENERATE_CLINICAL_SET { + take: + ch_vcf // channel: [mandatory] [ val(meta), path(vcf) ] + ch_vep_filters // channel: [mandatory] [ path(feature_file) ] + + main: + ch_versions = Channel.empty() + + ch_vcf + .multiMap { meta, vcf -> + clinical: [ meta + [ set: "clinical" ], vcf ] + research: [ meta + [ set: "research" ], vcf ] + } + .set { ch_clin_research_vcf } + + ENSEMBLVEP_FILTERVEP( + ch_clin_research_vcf.clinical, + ch_vep_filters + ) + + TABIX_BGZIP( ENSEMBLVEP_FILTERVEP.out.output ) + + ch_clin_research_vcf.research + .mix( TABIX_BGZIP.out.output ) + .set { ch_clin_research_split } + + TABIX_TABIX( ch_clin_research_split ) + + ch_versions = ch_versions.mix( ENSEMBLVEP_FILTERVEP.out.versions ) + ch_versions = ch_versions.mix( TABIX_BGZIP.out.versions ) + ch_versions = ch_versions.mix( TABIX_TABIX.out.versions ) + + emit: + vcf = ch_clin_research_split // channel: [ val(meta), path(vcf) ] + tbi = TABIX_TABIX.out.tbi // channel: [ val(meta), path(tbi) ] + versions = ch_versions // channel: [ path(versions.yml) ] +} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 9481b930..e3904671 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -107,38 +107,35 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custo include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' include { SMNCOPYNUMBERCALLER } from '../modules/nf-core/smncopynumbercaller/main' -include { ENSEMBLVEP_FILTERVEP as FILTERVEP_MT } from '../modules/nf-core/ensemblvep/filtervep' -include { ENSEMBLVEP_FILTERVEP as FILTERVEP_SNV } from '../modules/nf-core/ensemblvep/filtervep' -include { ENSEMBLVEP_FILTERVEP as FILTERVEP_SV } from '../modules/nf-core/ensemblvep/filtervep' -include { TABIX_BGZIPTABIX as BGZIPTABIX_MT } from '../modules/nf-core/tabix/bgziptabix' -include { TABIX_BGZIPTABIX as BGZIPTABIX_SNV } from '../modules/nf-core/tabix/bgziptabix' -include { TABIX_BGZIPTABIX as BGZIPTABIX_SV } from '../modules/nf-core/tabix/bgziptabix' // // SUBWORKFLOWS // -include { ALIGN } from '../subworkflows/local/align' -include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_MT } from '../subworkflows/local/annotate_consequence_pli' -include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SNV } from '../subworkflows/local/annotate_consequence_pli' -include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SV } from '../subworkflows/local/annotate_consequence_pli' -include { ANNOTATE_GENOME_SNVS } from '../subworkflows/local/annotate_genome_snvs' -include { ANNOTATE_MOBILE_ELEMENTS } from '../subworkflows/local/annotate_mobile_elements' -include { ANNOTATE_MT_SNVS } from '../subworkflows/local/annotate_mt_snvs' -include { ANNOTATE_STRUCTURAL_VARIANTS } from '../subworkflows/local/annotate_structural_variants' -include { CALL_REPEAT_EXPANSIONS } from '../subworkflows/local/call_repeat_expansions' -include { CALL_SNV } from '../subworkflows/local/call_snv' -include { CALL_STRUCTURAL_VARIANTS } from '../subworkflows/local/call_structural_variants' -include { GENERATE_CYTOSURE_FILES } from '../subworkflows/local/generate_cytosure_files' -include { GENS } from '../subworkflows/local/gens' -include { PEDDY_CHECK } from '../subworkflows/local/peddy_check' -include { PREPARE_REFERENCES } from '../subworkflows/local/prepare_references' -include { QC_BAM } from '../subworkflows/local/qc_bam' -include { RANK_VARIANTS as RANK_VARIANTS_MT } from '../subworkflows/local/rank_variants' -include { RANK_VARIANTS as RANK_VARIANTS_SNV } from '../subworkflows/local/rank_variants' -include { RANK_VARIANTS as RANK_VARIANTS_SV } from '../subworkflows/local/rank_variants' -include { SCATTER_GENOME } from '../subworkflows/local/scatter_genome' -include { CALL_MOBILE_ELEMENTS } from '../subworkflows/local/call_mobile_elements' +include { ALIGN } from '../subworkflows/local/align' +include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_MT } from '../subworkflows/local/annotate_consequence_pli' +include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SNV } from '../subworkflows/local/annotate_consequence_pli' +include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SV } from '../subworkflows/local/annotate_consequence_pli' +include { ANNOTATE_GENOME_SNVS } from '../subworkflows/local/annotate_genome_snvs' +include { ANNOTATE_MOBILE_ELEMENTS } from '../subworkflows/local/annotate_mobile_elements' +include { ANNOTATE_MT_SNVS } from '../subworkflows/local/annotate_mt_snvs' +include { ANNOTATE_STRUCTURAL_VARIANTS } from '../subworkflows/local/annotate_structural_variants' +include { CALL_REPEAT_EXPANSIONS } from '../subworkflows/local/call_repeat_expansions' +include { CALL_SNV } from '../subworkflows/local/call_snv' +include { CALL_STRUCTURAL_VARIANTS } from '../subworkflows/local/call_structural_variants' +include { GENERATE_CYTOSURE_FILES } from '../subworkflows/local/generate_cytosure_files' +include { GENERATE_CLINICAL_SET as GENERATE_CLINICAL_SET_MT } from '../subworkflows/local/generate_clinical_set' +include { GENERATE_CLINICAL_SET as GENERATE_CLINICAL_SET_SNV } from '../subworkflows/local/generate_clinical_set' +include { GENERATE_CLINICAL_SET as GENERATE_CLINICAL_SET_SV } from '../subworkflows/local/generate_clinical_set' +include { GENS } from '../subworkflows/local/gens' +include { PEDDY_CHECK } from '../subworkflows/local/peddy_check' +include { PREPARE_REFERENCES } from '../subworkflows/local/prepare_references' +include { QC_BAM } from '../subworkflows/local/qc_bam' +include { RANK_VARIANTS as RANK_VARIANTS_MT } from '../subworkflows/local/rank_variants' +include { RANK_VARIANTS as RANK_VARIANTS_SNV } from '../subworkflows/local/rank_variants' +include { RANK_VARIANTS as RANK_VARIANTS_SV } from '../subworkflows/local/rank_variants' +include { SCATTER_GENOME } from '../subworkflows/local/scatter_genome' +include { CALL_MOBILE_ELEMENTS } from '../subworkflows/local/call_mobile_elements' /* @@ -433,11 +430,17 @@ workflow RAREDISEASE { ch_vep_cache, ch_genome_fasta, ch_genome_dictionary - ).set {ch_sv_annotate} + ).set { ch_sv_annotate } ch_versions = ch_versions.mix(ch_sv_annotate.versions) - ANN_CSQ_PLI_SV ( + GENERATE_CLINICAL_SET_SV( ch_sv_annotate.vcf_ann, + ch_vep_filters + ) + ch_versions = ch_versions.mix(GENERATE_CLINICAL_SET_SV.out.versions) + + ANN_CSQ_PLI_SV ( + GENERATE_CLINICAL_SET_SV.out.vcf, ch_variant_consequences ) ch_versions = ch_versions.mix(ANN_CSQ_PLI_SV.out.versions) @@ -450,15 +453,6 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(RANK_VARIANTS_SV.out.versions) - FILTERVEP_SV( - RANK_VARIANTS_SV.out.vcf, - ch_vep_filters - ) - ch_versions = ch_versions.mix(FILTERVEP_SV.out.versions) - - BGZIPTABIX_SV(FILTERVEP_SV.out.output) - ch_versions = ch_versions.mix(BGZIPTABIX_SV.out.versions) - } // @@ -480,13 +474,17 @@ workflow RAREDISEASE { ch_genome_fasta, ch_gnomad_af, ch_scatter_split_intervals - ).set {ch_snv_annotate} + ).set { ch_snv_annotate } ch_versions = ch_versions.mix(ch_snv_annotate.versions) - ch_snv_annotate = ANNOTATE_GENOME_SNVS.out.vcf_ann + GENERATE_CLINICAL_SET_SNV( + ch_snv_annotate.vcf_ann, + ch_vep_filters + ) + ch_versions = ch_versions.mix(GENERATE_CLINICAL_SET_SNV.out.versions) ANN_CSQ_PLI_SNV ( - ch_snv_annotate, + GENERATE_CLINICAL_SET_SNV.out.vcf, ch_variant_consequences ) ch_versions = ch_versions.mix(ANN_CSQ_PLI_SNV.out.versions) @@ -499,15 +497,6 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(RANK_VARIANTS_SNV.out.versions) - FILTERVEP_SNV( - RANK_VARIANTS_SNV.out.vcf, - ch_vep_filters - ) - ch_versions = ch_versions.mix(FILTERVEP_SNV.out.versions) - - BGZIPTABIX_SNV(FILTERVEP_SNV.out.output) - ch_versions = ch_versions.mix(BGZIPTABIX_SNV.out.versions) - } // @@ -526,11 +515,17 @@ workflow RAREDISEASE { params.genome, params.vep_cache_version, ch_vep_cache, - ).set {ch_mt_annotate} + ).set { ch_mt_annotate } ch_versions = ch_versions.mix(ch_mt_annotate.versions) - ANN_CSQ_PLI_MT ( + GENERATE_CLINICAL_SET_MT( ch_mt_annotate.vcf_ann, + ch_vep_filters + ) + ch_versions = ch_versions.mix(GENERATE_CLINICAL_SET_MT.out.versions) + + ANN_CSQ_PLI_MT( + GENERATE_CLINICAL_SET_MT.out.vcf, ch_variant_consequences ) ch_versions = ch_versions.mix(ANN_CSQ_PLI_MT.out.versions) @@ -543,15 +538,6 @@ workflow RAREDISEASE { ) ch_versions = ch_versions.mix(RANK_VARIANTS_MT.out.versions) - FILTERVEP_MT( - RANK_VARIANTS_MT.out.vcf, - ch_vep_filters - ) - ch_versions = ch_versions.mix(FILTERVEP_MT.out.versions) - - BGZIPTABIX_MT(FILTERVEP_MT.out.output) - ch_versions = ch_versions.mix(BGZIPTABIX_MT.out.versions) - } // STEP 1.7: SMNCOPYNUMBERCALLER From a1e1950cc6848dd3edf21d067019fee20e4cae28 Mon Sep 17 00:00:00 2001 From: jemten Date: Mon, 22 Jan 2024 11:54:47 +0100 Subject: [PATCH 262/387] update changelogwq --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 93161c13..17c37e8c 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -43,6 +43,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Changed the name of the parameter from `skip_cnv_calling` to `skip_germlinecnvcaller` [#435](https://github.com/nf-core/raredisease/pull/435) - Check SVDB query input files for existence and correct format [#476](https://github.com/nf-core/raredisease/pull/476) - Change hardcoded platform value to params.platform in align_MT.config [#475](https://github.com/nf-core/raredisease/pull/475) +- The split into clincial and research VCFs is now done before ranking the varaints [#485](https://github.com/nf-core/raredisease/pull/485) ### `Fixed` From 1b1d6a125bef3a2179f35c5fb93494f54cfc43c4 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 22 Jan 2024 15:04:12 +0100 Subject: [PATCH 263/387] review suggestions --- assets/vep_plugin_files_schema.json | 19 +++++++++++++++++++ nextflow_schema.json | 4 +++- 2 files changed, 22 insertions(+), 1 deletion(-) create mode 100644 assets/vep_plugin_files_schema.json diff --git a/assets/vep_plugin_files_schema.json b/assets/vep_plugin_files_schema.json new file mode 100644 index 00000000..6f728a7b --- /dev/null +++ b/assets/vep_plugin_files_schema.json @@ -0,0 +1,19 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/raredisease/master/assets/mobile_element_references_schema.json", + "title": "Schema for VEP plugin files and their indices", + "description": "Schema for VEP plugin files and their indices", + "type": "array", + "items": { + "type": "object", + "properties": { + "vep_files": { + "type": "string", + "format": "file-path", + "exists": true, + "errorMessage": "Path to vep plugin files and their indices" + } + }, + "required": ["vep_files"] + } +} diff --git a/nextflow_schema.json b/nextflow_schema.json index 64056370..e1d952f1 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -390,7 +390,9 @@ "description": "Databases used by both named and custom plugins to annotate variants.", "fa_icon": "fas fa-file-csv", "help_text": "Path to a file containing the absolute paths to databases and their indices used by VEP's custom and named plugins resources defined within the vcfanno toml file. One line per resource.", - "mimetype": "text/csv" + "pattern": "^\\S+\\.csv$", + "mimetype": "text/csv", + "schema": "assets/vep_plugin_files_schema.json" }, "vep_filters": { "type": "string", From f1b75d4f8cceaae9968907946f968a721cf18191 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 22 Jan 2024 19:36:54 +0530 Subject: [PATCH 264/387] Apply suggestions from code review Co-authored-by: Anders Jemt --- subworkflows/local/annotate_genome_snvs.nf | 2 +- subworkflows/local/annotate_mobile_elements.nf | 2 +- subworkflows/local/annotate_mt_snvs.nf | 2 +- 3 files changed, 3 insertions(+), 3 deletions(-) diff --git a/subworkflows/local/annotate_genome_snvs.nf b/subworkflows/local/annotate_genome_snvs.nf index 291d3acd..9e3d74d6 100644 --- a/subworkflows/local/annotate_genome_snvs.nf +++ b/subworkflows/local/annotate_genome_snvs.nf @@ -117,7 +117,7 @@ workflow ANNOTATE_GENOME_SNVS { .set { ch_for_mix } ch_for_mix.selvar.mix(ch_for_mix.cadd) - .map { meta, vcf -> return [meta, vcf, []]} + .map { meta, vcf -> return [meta, vcf, []] } .set { ch_vep_in } diff --git a/subworkflows/local/annotate_mobile_elements.nf b/subworkflows/local/annotate_mobile_elements.nf index dc6247bf..265ccce0 100644 --- a/subworkflows/local/annotate_mobile_elements.nf +++ b/subworkflows/local/annotate_mobile_elements.nf @@ -55,7 +55,7 @@ workflow ANNOTATE_MOBILE_ELEMENTS { ch_genome_dictionary ) .vcf - .map { meta, vcf -> return [meta, vcf, []]} + .map { meta, vcf -> return [meta, vcf, []] } .set { ch_vep_in } ENSEMBLVEP_ME( diff --git a/subworkflows/local/annotate_mt_snvs.nf b/subworkflows/local/annotate_mt_snvs.nf index e1ed903a..e7b8ae6a 100644 --- a/subworkflows/local/annotate_mt_snvs.nf +++ b/subworkflows/local/annotate_mt_snvs.nf @@ -54,7 +54,7 @@ workflow ANNOTATE_MT_SNVS { ch_for_mix.merged.mix(ch_for_mix.cadd) .tap { ch_haplogrep_in } - .map { meta, vcf -> return [meta, vcf, []]} + .map { meta, vcf -> return [meta, vcf, []] } .set { ch_vep_in } From 778c54e8012ad433dbc25b78f40e793255df1bf3 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 24 Jan 2024 14:13:40 +0100 Subject: [PATCH 265/387] review suggestions + updates --- CITATIONS.md | 4 +++ README.md | 4 +++ ...uthvcfs.json => rtg_truthvcfs_schema.json} | 9 +++++- conf/modules/qc_bam.config | 4 +-- docs/output.md | 32 +++++++++++++++++++ docs/usage.md | 12 +++++++ nextflow_schema.json | 2 +- subworkflows/local/prepare_references.nf | 3 +- subworkflows/local/variant_evaluation.nf | 13 +++++--- workflows/raredisease.nf | 3 +- 10 files changed, 75 insertions(+), 11 deletions(-) rename assets/{rtg_truthvcfs.json => rtg_truthvcfs_schema.json} (78%) diff --git a/CITATIONS.md b/CITATIONS.md index f9973ae3..1fb40da9 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -106,6 +106,10 @@ - [rhocall](https://github.com/dnil/rhocall) +- [RTG Tools (vcfeval)](https://github.com/RealTimeGenomics/rtg-tools) + + > John G. Cleary, Ross Braithwaite, Kurt Gaastra, Brian S. Hilbush, Stuart Inglis, Sean A. Irvine, Alan Jackson, Richard Littin, Mehul Rathod, David Ware, Justin M. Zook, Len Trigg, and Francisco M. De La Vega. "Comparing Variant Call Files for Performance Benchmarking of Next-Generation Sequencing Variant Calling Pipelines." bioRxiv, 2015. doi:10.1101/023754. + - [Sentieon DNAscope](https://www.biorxiv.org/content/10.1101/2022.05.20.492556v1.abstract) > Freed D, Pan R, Chen H, Li Z, Hu J, Aldana R. DNAscope: High Accuracy Small Variant Calling Using Machine Learning. Bioinformatics; 2022. doi:10.1101/2022.05.20.492556 diff --git a/README.md b/README.md index 1e0a93ae..e0ba6cd9 100644 --- a/README.md +++ b/README.md @@ -102,6 +102,10 @@ On release, automated continuous integration tests run the pipeline on a full-si - [GENMOD](https://github.com/Clinical-Genomics/genmod) +**11. Variant evaluation:** + +- [RTG Tools](https://github.com/RealTimeGenomics/rtg-tools) + Note that it is possible to include/exclude certain tools or steps. ## Usage diff --git a/assets/rtg_truthvcfs.json b/assets/rtg_truthvcfs_schema.json similarity index 78% rename from assets/rtg_truthvcfs.json rename to assets/rtg_truthvcfs_schema.json index 3c3fe1db..b6ae4d06 100644 --- a/assets/rtg_truthvcfs.json +++ b/assets/rtg_truthvcfs_schema.json @@ -25,8 +25,15 @@ "exists": true, "pattern": "^\\S+\\.bed$", "errorMessage": "Path to bed files cannot contain spaces" + }, + "evaluationregions": { + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.bed$", + "errorMessage": "Path to bed files cannot contain spaces" } }, - "required": ["vcf", "samplename", "bedregions"] + "required": ["vcf", "samplename"] } } diff --git a/conf/modules/qc_bam.config b/conf/modules/qc_bam.config index 48e7d3c1..1982a757 100644 --- a/conf/modules/qc_bam.config +++ b/conf/modules/qc_bam.config @@ -65,12 +65,12 @@ process { } withName: '.*QC_BAM:PICARD_COLLECTWGSMETRICS' { - ext.when = { params.analysis_type.equals("wgs") && params.aligner.equals("bwamem2") } + ext.when = { params.analysis_type.equals("wgs") && !params.aligner.equals("sentieon") } ext.prefix = { "${meta.id}_wgsmetrics" } } withName: '.*QC_BAM:PICARD_COLLECTWGSMETRICS_Y' { - ext.when = { params.analysis_type.equals("wgs") && params.aligner.equals("bwamem2") } + ext.when = { params.analysis_type.equals("wgs") && !params.aligner.equals("sentieon") } ext.prefix = { "${meta.id}_wgsmetrics_y" } } diff --git a/docs/output.md b/docs/output.md index 885c806e..fd2cd30b 100644 --- a/docs/output.md +++ b/docs/output.md @@ -65,6 +65,10 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [VEP](#vep-2) - [Rank variants and filtering](#rank-variants-and-filtering) - [GENMOD](#genmod) + - [Mobile element analysis](#mobile-element-analysis) + - [Calling mobile elements](#calling-mobile-elements) + - [Annotating mobile elements](#annotating-mobile-elements) + - [Variant evaluation](#variant-evaluation) - [Pipeline information](#pipeline-information) ### Alignment @@ -522,6 +526,34 @@ The mobile elements are annotated with allele frequencies and allele counts usin
+### Variant evaluation + +Provided a truth set, SNVs can be evaluated using RTG Tools' vcfeval engine. Output files generated are listed below with a short description, but if you'd like to know more about what's in each of the files, refer to RTG Tools documentation [here](https://www.animalgenome.org/bioinfo/resources/manuals/RTGOperationsManual.pdf). + +
+Output files + +- `rtgvcfeval/` + + - `_vcfeval.fn.vcf.gz`: contains variants from the baseline VCF which were not correctly called. + - `_vcfeval.fn.vcf.gz.tbi`: index of the \*fn.vcf file + - `_vcfeval.fp.vcf.gz`: contains variants from the calls VCF which do not agree with baseline variants. + - `_vcfeval.fp.vcf.gz.tbi`: index of the \*fp.vcf file + - `_vcfeval.non_snp_roc.tsv.gz`: contains ROC data derived from those variants which were not represented as + SNPs. + - `_vcfeval.phasing.txt`: containing the data on the phasing + - `_vcfeval.snp_roc.tsv.gz`: contains ROC data derived from only those variants which were represented as SNPs. + - `_vcfeval.summary.txt`: contains the match summary statistics printed to standard output. + - `_vcfeval.tp-baseline.vcf.gz`: contains those variants from the baseline VCF which agree with variants in the + calls VCF. + - `_vcfeval.tp-baseline.vcf.gz.tbi`: index of the \*tp-baseline.vcf file + - `_vcfeval.tp.vcf.gz`: contains those variants from the calls VCF which agree with variants in the baseline VCF + - `_vcfeval.tp.vcf.gz.tbi`: index of the \*tp.vcf file + - `_vcfeval.weighted_roc.tsv.gz`: contains ROC data derived from all analyzed call variants, regardless of their + representation. + +
+ ### Pipeline information [Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. diff --git a/docs/usage.md b/docs/usage.md index 9c27621a..6dcff0b1 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -21,6 +21,8 @@ Table of contents: - [7. SNV annotation \& Ranking](#7-snv-annotation--ranking) - [8. SV annotation \& Ranking](#8-sv-annotation--ranking) - [9. Mitochondrial annotation](#9-mitochondrial-annotation) + - [10. Mobile element annoation](#10-mobile-element-annotation) + - [11. Variant evaluation](#11-variant-evaluation) - [Run the pipeline](#run-the-pipeline) - [Direct input in CLI](#direct-input-in-cli) - [Import from a config file (recommended)](#import-from-a-config-file-recommended) @@ -279,6 +281,16 @@ no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sampl 1 A CSV file that describes the databases (VCFs) used by SVDB for annotating mobile elements with allele frequencies. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/svdb_querydb_files.csv). +##### 11. Variant evaluation + +| Mandatory | Optional | +| -------------------------- | -------- | +| run_rtgvcfeval1 | sdf | +| rtg_truthvcfs2 | | + +1 This parameter is set to false by default, set it to true if if you'd like to run the evaluation subworkflow +2 A CSV file that describes the truth VCF files used by RTG Tools' vcfeval for evaluating SNVs. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/rtg_example.csv). The file contains four columns `samplename,vcf,bedregions,evaluationregions` where samplename is the user assigned samplename in the input samplesheet, vcf is the path to the truth vcf file, bedregions and evaluationregions are the path to the bed files that are supposed to be passed through --bed_regions and --evaluation_regions options of vcfeval. + #### Run the pipeline You can directly supply the parameters in the command line (CLI) or use a config file from which the pipeline can import the parameters. diff --git a/nextflow_schema.json b/nextflow_schema.json index 5c77956e..a3051514 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -280,7 +280,7 @@ "help_text": "Path to comma-separated file containing information about the truth vcf files used by vcfeval.", "pattern": "^\\S+\\.csv$", "mimetype": "text/csv", - "schema": "assets/rtg_truthvcfs.json" + "schema": "assets/rtg_truthvcfs_schema.json" }, "save_reference": { "type": "boolean", diff --git a/subworkflows/local/prepare_references.nf b/subworkflows/local/prepare_references.nf index b98b89d1..64d33248 100644 --- a/subworkflows/local/prepare_references.nf +++ b/subworkflows/local/prepare_references.nf @@ -52,7 +52,8 @@ workflow PREPARE_REFERENCES { GATK_SD(ch_genome_fasta) ch_fai = Channel.empty().mix(ch_genome_fai, SAMTOOLS_FAIDX_GENOME.out.fai).collect() GET_CHROM_SIZES( ch_fai ) - ch_genome_fasta.map {meta, fasta -> return [meta, fasta, [], [] ]}.set {ch_rtgformat_in} + ch_genome_fasta.map { meta, fasta -> return [meta, fasta, [], [] ] } + .set {ch_rtgformat_in} RTGTOOLS_FORMAT(ch_rtgformat_in) // MT indices diff --git a/subworkflows/local/variant_evaluation.nf b/subworkflows/local/variant_evaluation.nf index 8d550cdf..435b05d8 100644 --- a/subworkflows/local/variant_evaluation.nf +++ b/subworkflows/local/variant_evaluation.nf @@ -20,7 +20,9 @@ workflow VARIANT_EVALUATION { ch_rtg_truthvcfs .splitCsv ( header:true ) .map { row -> - return [[samplename:row.samplename[0], bedregions:row.bedregions[0]], row.vcf[0], []] + evregions = row.evaluationregions[0].isEmpty() ? [] : row.evaluationregions[0] + bedregions = row.bedregions[0].isEmpty() ? [] : row.bedregions[0] + return [[samplename:row.samplename[0], bedregions:bedregions, evaluationregions:evregions], row.vcf[0], []] } .set { ch_rtgvcfs_dbs } @@ -35,15 +37,16 @@ workflow VARIANT_EVALUATION { ch_snv_vcf_tbi .combine(ch_truthvcf_tbi) .map { meta, query, qidx, meta2, truth, tidx -> - return [meta + [samplename: meta2.samplename] , query, qidx, truth, tidx, meta2.bed, []] + return [meta + [samplename: meta2.samplename] , query, qidx, truth, tidx, meta2.bedregions, meta2.evaluationregions] } .set { ch_vcfeval_in } RTGTOOLS_VCFEVAL ( ch_vcfeval_in, ch_sdf ) - // ch_versions = ch_versions.mix(BUILD_BED.out.versions) - // ch_versions = ch_versions.mix(GATK4_SPLITINTERVALS.out.versions) + ch_versions = ch_versions.mix(BCFTOOLS_REHEADER.out.versions) + ch_versions = ch_versions.mix(TABIX_TRUTHVCF.out.versions) + ch_versions = ch_versions.mix(RTGTOOLS_VCFEVAL.out.versions) emit: - versions = ch_versions // channel: [ path(versions.yml) ] + versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 8ddccb1f..49a6853c 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -280,7 +280,8 @@ workflow RAREDISEASE { : Channel.value([]) ch_score_config_sv = params.score_config_sv ? Channel.fromPath(params.score_config_sv).collect() : Channel.value([]) - ch_sdf = ch_references.sdf + ch_sdf = params.sdf ? Channel.fromPath(params.sdf).map{it -> [[id:it[0].simpleName],it]}.collect() + : ch_references.sdf ch_sv_dbs = params.svdb_query_dbs ? Channel.fromPath(params.svdb_query_dbs) : Channel.empty() ch_sv_bedpedbs = params.svdb_query_bedpedbs ? Channel.fromPath(params.svdb_query_bedpedbs) From 1b7ba7f0ca60a903eb07f3319a993273a9a8ac9c Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 24 Jan 2024 14:15:53 +0100 Subject: [PATCH 266/387] add versions --- workflows/raredisease.nf | 1 + 1 file changed, 1 insertion(+) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 49a6853c..5e3ff3ef 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -415,6 +415,7 @@ workflow RAREDISEASE { ch_sdf ) } + ch_versions = ch_versions.mix(VARIANT_EVALUATION.out.versions) // // SV CALLING From db3d45a0241263857858e74a103b375e67523117 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 24 Jan 2024 14:20:35 +0100 Subject: [PATCH 267/387] fix error --- workflows/raredisease.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 73b73bf4..8f043395 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -430,8 +430,8 @@ workflow RAREDISEASE { ch_rtg_truthvcfs, ch_sdf ) + ch_versions = ch_versions.mix(VARIANT_EVALUATION.out.versions) } - ch_versions = ch_versions.mix(VARIANT_EVALUATION.out.versions) // // SV CALLING From 2427ddc6c0b8b985290f0be60f9861df30e1ba44 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 24 Jan 2024 22:16:29 +0100 Subject: [PATCH 268/387] modules updaate --- .devcontainer/devcontainer.json | 8 +- modules.json | 180 +++++------ modules/nf-core/bcftools/annotate/main.nf | 6 +- modules/nf-core/bcftools/annotate/meta.yml | 6 +- modules/nf-core/bcftools/concat/main.nf | 6 +- modules/nf-core/bcftools/concat/meta.yml | 8 +- modules/nf-core/bcftools/filter/main.nf | 6 +- modules/nf-core/bcftools/filter/meta.yml | 3 + modules/nf-core/bcftools/merge/main.nf | 6 +- modules/nf-core/bcftools/merge/meta.yml | 5 + modules/nf-core/bcftools/norm/main.nf | 6 +- modules/nf-core/bcftools/norm/meta.yml | 3 + modules/nf-core/bcftools/reheader/main.nf | 18 +- modules/nf-core/bcftools/reheader/meta.yml | 11 +- modules/nf-core/bcftools/roh/main.nf | 6 +- modules/nf-core/bcftools/roh/meta.yml | 8 +- modules/nf-core/bcftools/sort/main.nf | 7 +- modules/nf-core/bcftools/sort/meta.yml | 5 +- modules/nf-core/bcftools/view/main.nf | 6 +- modules/nf-core/bcftools/view/meta.yml | 3 +- modules/nf-core/bwa/index/main.nf | 18 +- modules/nf-core/bwa/index/meta.yml | 3 + modules/nf-core/bwamem2/index/main.nf | 17 +- modules/nf-core/bwamem2/index/meta.yml | 2 + modules/nf-core/bwamem2/mem/main.nf | 6 +- modules/nf-core/bwamem2/mem/meta.yml | 2 + modules/nf-core/cadd/main.nf | 2 +- modules/nf-core/cadd/meta.yml | 5 +- modules/nf-core/cat/cat/main.nf | 11 +- modules/nf-core/cat/cat/tests/main.nf.test | 6 +- .../nf-core/cat/cat/tests/main.nf.test.snap | 92 ++++-- modules/nf-core/chromograph/main.nf | 2 +- modules/nf-core/chromograph/meta.yml | 5 +- modules/nf-core/cnvnator/cnvnator/main.nf | 2 +- modules/nf-core/cnvnator/cnvnator/meta.yml | 5 +- modules/nf-core/cnvnator/convert2vcf/main.nf | 2 +- modules/nf-core/cnvnator/convert2vcf/meta.yml | 5 +- .../dumpsoftwareversions/environment.yml | 2 +- .../custom/dumpsoftwareversions/main.nf | 4 +- .../dumpsoftwareversions/tests/main.nf.test | 7 +- .../tests/main.nf.test.snap | 50 +-- modules/nf-core/deepvariant/meta.yml | 6 +- modules/nf-core/ensemblvep/filtervep/main.nf | 2 +- modules/nf-core/ensemblvep/filtervep/meta.yml | 7 +- modules/nf-core/expansionhunter/main.nf | 6 +- modules/nf-core/expansionhunter/meta.yml | 5 +- modules/nf-core/fastp/main.nf | 18 ++ modules/nf-core/fastp/tests/main.nf.test | 302 ++++++++++++++++-- modules/nf-core/fastp/tests/main.nf.test.snap | 63 +++- modules/nf-core/fastqc/tests/main.nf.test | 265 ++++++++++----- .../nf-core/fastqc/tests/main.nf.test.snap | 12 +- .../nf-core/gatk4/bedtointervallist/main.nf | 5 +- .../nf-core/gatk4/bedtointervallist/meta.yml | 6 +- .../nf-core/gatk4/collectreadcounts/main.nf | 5 +- .../nf-core/gatk4/collectreadcounts/meta.yml | 13 +- .../gatk4/createsequencedictionary/main.nf | 5 +- .../gatk4/createsequencedictionary/meta.yml | 7 +- .../nf-core/gatk4/denoisereadcounts/main.nf | 5 +- .../nf-core/gatk4/denoisereadcounts/meta.yml | 9 +- .../determinegermlinecontigploidy/main.nf | 3 +- .../determinegermlinecontigploidy/meta.yml | 11 +- .../nf-core/gatk4/filtermutectcalls/main.nf | 5 +- .../nf-core/gatk4/filtermutectcalls/meta.yml | 12 +- .../nf-core/gatk4/germlinecnvcaller/main.nf | 9 +- .../nf-core/gatk4/germlinecnvcaller/meta.yml | 27 +- .../nf-core/gatk4/intervallisttools/main.nf | 5 +- .../nf-core/gatk4/intervallisttools/meta.yml | 12 +- .../nf-core/gatk4/mergebamalignment/main.nf | 5 +- .../nf-core/gatk4/mergebamalignment/meta.yml | 4 + modules/nf-core/gatk4/mergevcfs/main.nf | 5 +- modules/nf-core/gatk4/mergevcfs/meta.yml | 7 +- modules/nf-core/gatk4/mutect2/main.nf | 5 +- modules/nf-core/gatk4/mutect2/meta.yml | 10 +- .../gatk4/postprocessgermlinecnvcalls/main.nf | 3 +- .../postprocessgermlinecnvcalls/meta.yml | 7 +- .../nf-core/gatk4/preprocessintervals/main.nf | 5 +- .../gatk4/preprocessintervals/meta.yml | 16 +- modules/nf-core/gatk4/printreads/main.nf | 5 +- modules/nf-core/gatk4/printreads/meta.yml | 9 +- modules/nf-core/gatk4/revertsam/main.nf | 5 +- modules/nf-core/gatk4/revertsam/meta.yml | 5 +- modules/nf-core/gatk4/samtofastq/main.nf | 5 +- modules/nf-core/gatk4/samtofastq/meta.yml | 5 +- modules/nf-core/gatk4/selectvariants/main.nf | 5 +- modules/nf-core/gatk4/selectvariants/meta.yml | 7 +- modules/nf-core/gatk4/shiftfasta/main.nf | 5 +- modules/nf-core/gatk4/shiftfasta/meta.yml | 8 +- modules/nf-core/gatk4/splitintervals/main.nf | 5 +- modules/nf-core/gatk4/splitintervals/meta.yml | 9 +- .../nf-core/gatk4/variantfiltration/main.nf | 5 +- .../nf-core/gatk4/variantfiltration/meta.yml | 6 +- modules/nf-core/genmod/annotate/main.nf | 2 +- modules/nf-core/genmod/annotate/meta.yml | 4 +- modules/nf-core/genmod/compound/main.nf | 2 +- modules/nf-core/genmod/compound/meta.yml | 4 +- modules/nf-core/genmod/models/main.nf | 2 +- modules/nf-core/genmod/models/meta.yml | 5 +- modules/nf-core/genmod/score/main.nf | 2 +- modules/nf-core/genmod/score/meta.yml | 5 +- modules/nf-core/glnexus/main.nf | 2 +- modules/nf-core/glnexus/meta.yml | 5 +- modules/nf-core/haplocheck/main.nf | 2 +- modules/nf-core/haplocheck/meta.yml | 9 +- modules/nf-core/haplogrep2/classify/main.nf | 2 +- modules/nf-core/haplogrep2/classify/meta.yml | 6 +- modules/nf-core/hmtnote/annotate/main.nf | 2 +- modules/nf-core/hmtnote/annotate/meta.yml | 6 +- modules/nf-core/manta/germline/main.nf | 27 +- modules/nf-core/manta/germline/meta.yml | 12 +- modules/nf-core/mosdepth/main.nf | 6 +- modules/nf-core/mosdepth/meta.yml | 14 +- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 6 +- modules/nf-core/multiqc/meta.yml | 1 - modules/nf-core/multiqc/tests/main.nf.test | 48 ++- modules/nf-core/peddy/main.nf | 2 +- modules/nf-core/peddy/meta.yml | 6 +- .../picard/addorreplacereadgroups/main.nf | 11 +- .../picard/addorreplacereadgroups/meta.yml | 10 +- .../nf-core/picard/collecthsmetrics/main.nf | 6 +- .../nf-core/picard/collecthsmetrics/meta.yml | 5 +- .../picard/collectmultiplemetrics/main.nf | 8 +- .../picard/collectmultiplemetrics/meta.yml | 2 + .../nf-core/picard/collectwgsmetrics/main.nf | 6 +- .../nf-core/picard/collectwgsmetrics/meta.yml | 5 + modules/nf-core/picard/liftovervcf/main.nf | 6 +- modules/nf-core/picard/liftovervcf/meta.yml | 13 +- modules/nf-core/picard/markduplicates/main.nf | 10 +- .../nf-core/picard/markduplicates/meta.yml | 4 + .../nf-core/picard/renamesampleinvcf/main.nf | 6 +- .../nf-core/picard/renamesampleinvcf/meta.yml | 9 +- modules/nf-core/picard/sortvcf/main.nf | 6 +- modules/nf-core/picard/sortvcf/meta.yml | 5 +- modules/nf-core/qualimap/bamqc/main.nf | 6 +- modules/nf-core/qualimap/bamqc/meta.yml | 4 +- modules/nf-core/rhocall/annotate/main.nf | 2 +- modules/nf-core/rhocall/annotate/meta.yml | 6 +- modules/nf-core/samtools/faidx/main.nf | 6 +- modules/nf-core/samtools/faidx/meta.yml | 4 + modules/nf-core/samtools/index/main.nf | 6 +- modules/nf-core/samtools/index/meta.yml | 4 + modules/nf-core/samtools/merge/main.nf | 11 +- modules/nf-core/samtools/merge/meta.yml | 10 + modules/nf-core/samtools/sort/main.nf | 6 +- modules/nf-core/samtools/sort/meta.yml | 3 + modules/nf-core/samtools/stats/main.nf | 6 +- modules/nf-core/samtools/stats/meta.yml | 4 + modules/nf-core/samtools/view/main.nf | 19 +- modules/nf-core/samtools/view/meta.yml | 5 + modules/nf-core/sentieon/bwaindex/main.nf | 31 +- modules/nf-core/sentieon/bwaindex/meta.yml | 3 + modules/nf-core/sentieon/bwamem/main.nf | 29 +- modules/nf-core/sentieon/bwamem/meta.yml | 2 + modules/nf-core/sentieon/datametrics/main.nf | 29 +- modules/nf-core/sentieon/datametrics/meta.yml | 7 +- modules/nf-core/sentieon/dedup/main.nf | 39 ++- modules/nf-core/sentieon/dedup/meta.yml | 2 + .../nf-core/sentieon/dnamodelapply/main.nf | 34 +- .../nf-core/sentieon/dnamodelapply/meta.yml | 7 +- modules/nf-core/sentieon/dnascope/main.nf | 34 +- modules/nf-core/sentieon/dnascope/meta.yml | 3 +- modules/nf-core/sentieon/readwriter/main.nf | 29 +- modules/nf-core/sentieon/readwriter/meta.yml | 4 +- modules/nf-core/sentieon/wgsmetrics/main.nf | 31 +- modules/nf-core/sentieon/wgsmetrics/meta.yml | 7 +- modules/nf-core/smncopynumbercaller/main.nf | 2 +- modules/nf-core/smncopynumbercaller/meta.yml | 5 +- modules/nf-core/stranger/main.nf | 2 +- modules/nf-core/stranger/meta.yml | 5 +- modules/nf-core/svdb/merge/main.nf | 2 +- modules/nf-core/svdb/merge/meta.yml | 2 + modules/nf-core/tabix/bgzip/main.nf | 2 +- modules/nf-core/tabix/bgzip/meta.yml | 5 + modules/nf-core/tabix/bgziptabix/main.nf | 2 +- modules/nf-core/tabix/bgziptabix/meta.yml | 3 + modules/nf-core/tabix/tabix/main.nf | 2 +- modules/nf-core/tabix/tabix/meta.yml | 4 + modules/nf-core/tiddit/sv/main.nf | 2 +- modules/nf-core/tiddit/sv/meta.yml | 2 + modules/nf-core/ucsc/wigtobigwig/main.nf | 2 +- modules/nf-core/ucsc/wigtobigwig/meta.yml | 6 +- modules/nf-core/untar/main.nf | 2 +- modules/nf-core/untar/meta.yml | 5 + modules/nf-core/upd/main.nf | 2 +- modules/nf-core/upd/meta.yml | 2 + modules/nf-core/vcfanno/main.nf | 6 +- modules/nf-core/vcfanno/meta.yml | 7 +- 187 files changed, 1541 insertions(+), 751 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4a9bc5c7..4ecfbfe3 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,11 +18,11 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint", + "python.linting.pylintPath": "/opt/conda/bin/pylint" }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], - }, - }, + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] + } + } } diff --git a/modules.json b/modules.json index 03000a35..c386c370 100644 --- a/modules.json +++ b/modules.json @@ -7,52 +7,52 @@ "nf-core": { "bcftools/annotate": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, "bcftools/concat": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, "bcftools/filter": { "branch": "master", - "git_sha": "bd4e0df3319c171072d09dade42e3c06fa373779", + "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, "bcftools/merge": { "branch": "master", - "git_sha": "f7219b428dc69f93aa19f219fb7ce8eae8720400", + "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, "bcftools/norm": { "branch": "master", - "git_sha": "0435e4eebc94e53721c194b2d5d06f455a79e407", + "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, "bcftools/reheader": { "branch": "master", - "git_sha": "0435e4eebc94e53721c194b2d5d06f455a79e407", + "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, "bcftools/roh": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, "bcftools/sort": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, "bcftools/view": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, "bwa/index": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "bwa/mem": { @@ -62,47 +62,47 @@ }, "bwamem2/index": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "bwamem2/mem": { "branch": "master", - "git_sha": "0460d316170f75f323111b4a2c0a2989f0c32013", + "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", "installed_by": ["modules"] }, "cadd": { "branch": "master", - "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "cat/cat": { "branch": "master", - "git_sha": "d593e8f6b7d1bbbb2acf43a4b9efeeac8d6720f2", + "git_sha": "81f27e75847087865299cc46605deb3b09b4e0a2", "installed_by": ["modules"] }, "chromograph": { "branch": "master", - "git_sha": "aad210ba51500be029740d088b4b4827f6f41509", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "cnvnator/cnvnator": { "branch": "master", - "git_sha": "e0fcafde1e74156695fc27e90a6ad6325065e6c5", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "cnvnator/convert2vcf": { "branch": "master", - "git_sha": "e0fcafde1e74156695fc27e90a6ad6325065e6c5", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] }, "deepvariant": { "branch": "master", - "git_sha": "ed67f2fadd6d2a155b296f728e6b1f8c92ddc1a6", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "eklipse": { @@ -112,7 +112,7 @@ }, "ensemblvep/filtervep": { "branch": "master", - "git_sha": "29984d70aea47d06f0062a1785d76c357dd40ea9", + "git_sha": "214d575774c172062924ad3564b4f66655600730", "installed_by": ["modules"] }, "ensemblvep/vep": { @@ -122,174 +122,174 @@ }, "expansionhunter": { "branch": "master", - "git_sha": "0260e5d22372eae434816d6970dedf3f5adc0053", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "fastp": { "branch": "master", - "git_sha": "3c77ca9aac783e76c3614a06db3bfe4fef619bde", + "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", + "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", "installed_by": ["modules"] }, "gatk4/bedtointervallist": { "branch": "master", - "git_sha": "2df2a11d5b12f2a73bca74f103691bc35d83c5fd", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/collectreadcounts": { "branch": "master", - "git_sha": "d25bf48327e86a7f737047a57ec264b90e22ce3d", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/createsequencedictionary": { "branch": "master", - "git_sha": "541811d779026c5d395925895fa5ed35e7216cc0", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/denoisereadcounts": { "branch": "master", - "git_sha": "38c559b2a8b9a8cda62fa215521f581eb979c773", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/determinegermlinecontigploidy": { "branch": "master", - "git_sha": "8c4542e5d421c4690cf1fa6ec729e9304763fdaf", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/filtermutectcalls": { "branch": "master", - "git_sha": "2df2a11d5b12f2a73bca74f103691bc35d83c5fd", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/germlinecnvcaller": { "branch": "master", - "git_sha": "8c4542e5d421c4690cf1fa6ec729e9304763fdaf", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"], "patch": "modules/nf-core/gatk4/germlinecnvcaller/gatk4-germlinecnvcaller.diff" }, "gatk4/intervallisttools": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/mergebamalignment": { "branch": "master", - "git_sha": "2df2a11d5b12f2a73bca74f103691bc35d83c5fd", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/mergevcfs": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/mutect2": { "branch": "master", - "git_sha": "2df2a11d5b12f2a73bca74f103691bc35d83c5fd", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/postprocessgermlinecnvcalls": { "branch": "master", - "git_sha": "8c4542e5d421c4690cf1fa6ec729e9304763fdaf", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"], "patch": "modules/nf-core/gatk4/postprocessgermlinecnvcalls/gatk4-postprocessgermlinecnvcalls.diff" }, "gatk4/preprocessintervals": { "branch": "master", - "git_sha": "1226419498a14d17f98d12d6488d333b0dbd0418", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/printreads": { "branch": "master", - "git_sha": "541811d779026c5d395925895fa5ed35e7216cc0", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/revertsam": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/samtofastq": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/selectvariants": { "branch": "master", - "git_sha": "541811d779026c5d395925895fa5ed35e7216cc0", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/shiftfasta": { "branch": "master", - "git_sha": "541811d779026c5d395925895fa5ed35e7216cc0", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/splitintervals": { "branch": "master", - "git_sha": "541811d779026c5d395925895fa5ed35e7216cc0", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "gatk4/variantfiltration": { "branch": "master", - "git_sha": "2df2a11d5b12f2a73bca74f103691bc35d83c5fd", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "genmod/annotate": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "genmod/compound": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "genmod/models": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "genmod/score": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "glnexus": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "haplocheck": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "haplogrep2/classify": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "hmtnote/annotate": { "branch": "master", - "git_sha": "a746b933e61f43f8932aa2f867d5ec7f0ded352b", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "manta/germline": { "branch": "master", - "git_sha": "80dbd95c558a0ebb2123d95f50c093a7f714a0d7", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "mosdepth": { "branch": "master", - "git_sha": "ebb27711cd5f4de921244bfa81c676504072d31c", + "git_sha": "69e3eb17fb31b772b18f134d6e8f8b93ee980e65", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "4ab13872435962dadc239979554d13709e20bf29", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] }, "ngsbits/samplegender": { @@ -299,142 +299,142 @@ }, "peddy": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "picard/addorreplacereadgroups": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447", "installed_by": ["modules"] }, "picard/collecthsmetrics": { "branch": "master", - "git_sha": "0ce3ab0ac301f160225b22254fa238478b4389f2", + "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447", "installed_by": ["modules"] }, "picard/collectmultiplemetrics": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447", "installed_by": ["modules"] }, "picard/collectwgsmetrics": { "branch": "master", - "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", + "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447", "installed_by": ["modules"] }, "picard/liftovervcf": { "branch": "master", - "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", + "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447", "installed_by": ["modules"] }, "picard/markduplicates": { "branch": "master", - "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", + "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447", "installed_by": ["modules"] }, "picard/renamesampleinvcf": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447", "installed_by": ["modules"] }, "picard/sortvcf": { "branch": "master", - "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", + "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447", "installed_by": ["modules"] }, "qualimap/bamqc": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "260677adfe5eecf5f64d3db726f6854d4497b49b", "installed_by": ["modules"] }, "rhocall/annotate": { "branch": "master", - "git_sha": "d73505dd68b27b53b4002e84eea21a2819907562", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "samtools/faidx": { "branch": "master", - "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe", + "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", "installed_by": ["modules"] }, "samtools/index": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", "installed_by": ["modules"] }, "samtools/merge": { "branch": "master", - "git_sha": "0460d316170f75f323111b4a2c0a2989f0c32013", + "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", "installed_by": ["modules"] }, "samtools/sort": { "branch": "master", - "git_sha": "a0f7be95788366c1923171e358da7d049eb440f9", + "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", "installed_by": ["modules"] }, "samtools/stats": { "branch": "master", - "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", + "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", "installed_by": ["modules"] }, "samtools/view": { "branch": "master", - "git_sha": "3ffae3598260a99e8db3207dead9f73f87f90d1f", + "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", "installed_by": ["modules"] }, "sentieon/bwaindex": { "branch": "master", - "git_sha": "49af8ed458e28729e483bc96e5a57c28163b9ea0", + "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", "installed_by": ["modules"] }, "sentieon/bwamem": { "branch": "master", - "git_sha": "149b4746c6e16ef84f64db5bb245d5b9495fdc3f", + "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", "installed_by": ["modules"] }, "sentieon/datametrics": { "branch": "master", - "git_sha": "127edadc279e19da093fdd513926c6cdee82c306", + "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", "installed_by": ["modules"] }, "sentieon/dedup": { "branch": "master", - "git_sha": "915a0b16ba3e40ef59e7b44843b3118e17a9c906", + "git_sha": "740119f83a28777fdd846da87196b6ebc32a0e1d", "installed_by": ["modules"] }, "sentieon/dnamodelapply": { "branch": "master", - "git_sha": "43ef68091a1188fd8dc4c03f9341b556803c7514", + "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", "installed_by": ["modules"] }, "sentieon/dnascope": { "branch": "master", - "git_sha": "4fb6fdc8046ec09cd30f92a2a252e9a0ba4a6309", + "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", "installed_by": ["modules"] }, "sentieon/readwriter": { "branch": "master", - "git_sha": "b28e4dde755117e8dab5d6e85e292f145b8b53c3", + "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", "installed_by": ["modules"] }, "sentieon/wgsmetrics": { "branch": "master", - "git_sha": "b1e9314b919f498e116bfc1417b7185185e99261", + "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", "installed_by": ["modules"] }, "smncopynumbercaller": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "stranger": { "branch": "master", - "git_sha": "0260e5d22372eae434816d6970dedf3f5adc0053", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "svdb/merge": { "branch": "master", - "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "svdb/query": { @@ -444,17 +444,17 @@ }, "tabix/bgzip": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "tabix/bgziptabix": { "branch": "master", - "git_sha": "591b71642820933dcb3c954c934b397bd00d8e5e", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "tabix/tabix": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "tiddit/cov": { @@ -464,22 +464,22 @@ }, "tiddit/sv": { "branch": "master", - "git_sha": "1c90a501d102b800c27697f5ef39a6e217ab1915", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "ucsc/wigtobigwig": { "branch": "master", - "git_sha": "66290981ab6038ea86177ade40b9449bc790b0ce", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "untar": { "branch": "master", - "git_sha": "d0b4fc03af52a1cc8c6fb4493b921b57352b1dd8", + "git_sha": "e719354ba77df0a1bd310836aa2039b45c29d620", "installed_by": ["modules"] }, "upd": { "branch": "master", - "git_sha": "9b159849d74f0eef251168c81c16da08215bbad5", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "vcf2cytosure": { @@ -489,7 +489,7 @@ }, "vcfanno": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "b558cd24f8751dcda51f957391f5f9cb83e28586", "installed_by": ["modules"] } } diff --git a/modules/nf-core/bcftools/annotate/main.nf b/modules/nf-core/bcftools/annotate/main.nf index 49eec2e8..27ebfd4a 100644 --- a/modules/nf-core/bcftools/annotate/main.nf +++ b/modules/nf-core/bcftools/annotate/main.nf @@ -2,10 +2,10 @@ process BCFTOOLS_ANNOTATE { tag "$meta.id" label 'process_low' - conda "bioconda::bcftools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': - 'biocontainers/bcftools:1.17--haef29d1_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'biocontainers/bcftools:1.18--h8b25389_0' }" input: tuple val(meta), path(input), path(index), path(annotations), path(annotations_index), path(header_lines) diff --git a/modules/nf-core/bcftools/annotate/meta.yml b/modules/nf-core/bcftools/annotate/meta.yml index 60f053ea..f3aa463b 100644 --- a/modules/nf-core/bcftools/annotate/meta.yml +++ b/modules/nf-core/bcftools/annotate/meta.yml @@ -13,7 +13,6 @@ tools: documentation: https://samtools.github.io/bcftools/bcftools.html#annotate doi: 10.1093/bioinformatics/btp352 licence: ["MIT"] - input: - meta: type: map @@ -35,7 +34,6 @@ input: - header_lines: type: file description: Contains lines to append to the output VCF header - output: - meta: type: map @@ -50,7 +48,9 @@ output: type: file description: Compressed annotated VCF file pattern: "*{vcf,vcf.gz,bcf,bcf.gz}" - authors: - "@projectoriented" - "@ramprasadn" +maintainers: + - "@projectoriented" + - "@ramprasadn" diff --git a/modules/nf-core/bcftools/concat/main.nf b/modules/nf-core/bcftools/concat/main.nf index 244a42cc..a5830a25 100644 --- a/modules/nf-core/bcftools/concat/main.nf +++ b/modules/nf-core/bcftools/concat/main.nf @@ -2,10 +2,10 @@ process BCFTOOLS_CONCAT { tag "$meta.id" label 'process_medium' - conda "bioconda::bcftools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': - 'biocontainers/bcftools:1.17--haef29d1_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'biocontainers/bcftools:1.18--h8b25389_0' }" input: tuple val(meta), path(vcfs), path(tbi) diff --git a/modules/nf-core/bcftools/concat/meta.yml b/modules/nf-core/bcftools/concat/meta.yml index e8c83cd6..91cb54d5 100644 --- a/modules/nf-core/bcftools/concat/meta.yml +++ b/modules/nf-core/bcftools/concat/meta.yml @@ -5,7 +5,6 @@ keywords: - concat - bcftools - VCF - tools: - concat: description: | @@ -21,12 +20,12 @@ input: Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - vcfs: - type: files + type: list description: | List containing 2 or more vcf files e.g. [ 'file1.vcf', 'file2.vcf' ] - tbi: - type: files + type: list description: | List containing 2 or more index files (optional) e.g. [ 'file1.tbi', 'file2.tbi' ] @@ -47,3 +46,6 @@ output: authors: - "@abhi18av" - "@nvnieuwk" +maintainers: + - "@abhi18av" + - "@nvnieuwk" diff --git a/modules/nf-core/bcftools/filter/main.nf b/modules/nf-core/bcftools/filter/main.nf index 099eedc7..1a40cb93 100644 --- a/modules/nf-core/bcftools/filter/main.nf +++ b/modules/nf-core/bcftools/filter/main.nf @@ -2,10 +2,10 @@ process BCFTOOLS_FILTER { tag "$meta.id" label 'process_medium' - conda "bioconda::bcftools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': - 'biocontainers/bcftools:1.17--haef29d1_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'biocontainers/bcftools:1.18--h8b25389_0' }" input: tuple val(meta), path(vcf) diff --git a/modules/nf-core/bcftools/filter/meta.yml b/modules/nf-core/bcftools/filter/meta.yml index 05a6d828..5b111fc3 100644 --- a/modules/nf-core/bcftools/filter/meta.yml +++ b/modules/nf-core/bcftools/filter/meta.yml @@ -39,3 +39,6 @@ output: authors: - "@joseespinosa" - "@drpatelh" +maintainers: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/bcftools/merge/main.nf b/modules/nf-core/bcftools/merge/main.nf index eec740ed..b85d3fec 100644 --- a/modules/nf-core/bcftools/merge/main.nf +++ b/modules/nf-core/bcftools/merge/main.nf @@ -2,10 +2,10 @@ process BCFTOOLS_MERGE { tag "$meta.id" label 'process_medium' - conda "bioconda::bcftools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': - 'biocontainers/bcftools:1.17--haef29d1_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'biocontainers/bcftools:1.18--h8b25389_0' }" input: tuple val(meta), path(vcfs), path(tbis) diff --git a/modules/nf-core/bcftools/merge/meta.yml b/modules/nf-core/bcftools/merge/meta.yml index 7bbe5216..87707140 100644 --- a/modules/nf-core/bcftools/merge/meta.yml +++ b/modules/nf-core/bcftools/merge/meta.yml @@ -81,3 +81,8 @@ authors: - "@drpatelh" - "@nvnieuwk" - "@ramprasadn" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@nvnieuwk" + - "@ramprasadn" diff --git a/modules/nf-core/bcftools/norm/main.nf b/modules/nf-core/bcftools/norm/main.nf index 608f20a1..47d3dab1 100644 --- a/modules/nf-core/bcftools/norm/main.nf +++ b/modules/nf-core/bcftools/norm/main.nf @@ -2,10 +2,10 @@ process BCFTOOLS_NORM { tag "$meta.id" label 'process_medium' - conda "bioconda::bcftools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': - 'biocontainers/bcftools:1.17--haef29d1_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'biocontainers/bcftools:1.18--h8b25389_0' }" input: tuple val(meta), path(vcf), path(tbi) diff --git a/modules/nf-core/bcftools/norm/meta.yml b/modules/nf-core/bcftools/norm/meta.yml index 33ebea36..1f3e1b62 100644 --- a/modules/nf-core/bcftools/norm/meta.yml +++ b/modules/nf-core/bcftools/norm/meta.yml @@ -56,3 +56,6 @@ output: authors: - "@abhi18av" - "@ramprasadn" +maintainers: + - "@abhi18av" + - "@ramprasadn" diff --git a/modules/nf-core/bcftools/reheader/main.nf b/modules/nf-core/bcftools/reheader/main.nf index 28d567a2..82527167 100644 --- a/modules/nf-core/bcftools/reheader/main.nf +++ b/modules/nf-core/bcftools/reheader/main.nf @@ -2,13 +2,13 @@ process BCFTOOLS_REHEADER { tag "$meta.id" label 'process_low' - conda "bioconda::bcftools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': - 'biocontainers/bcftools:1.17--haef29d1_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'biocontainers/bcftools:1.18--h8b25389_0' }" input: - tuple val(meta), path(vcf), path(header) + tuple val(meta), path(vcf), path(header), path(samples) tuple val(meta2), path(fai) output: @@ -21,8 +21,9 @@ process BCFTOOLS_REHEADER { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def update_sequences = fai ? "-f $fai" : "" - def new_header = header ? "-h $header" : "" + def fai_argument = fai ? "--fai $fai" : "" + def header_argument = header ? "--header $header" : "" + def samples_argument = samples ? "--samples $samples" : "" def args2 = task.ext.args2 ?: '--output-type z' def extension = args2.contains("--output-type b") || args2.contains("-Ob") ? "bcf.gz" : @@ -33,8 +34,9 @@ process BCFTOOLS_REHEADER { """ bcftools \\ reheader \\ - $update_sequences \\ - $new_header \\ + $fai_argument \\ + $header_argument \\ + $samples_argument \\ $args \\ --threads $task.cpus \\ $vcf \\ diff --git a/modules/nf-core/bcftools/reheader/meta.yml b/modules/nf-core/bcftools/reheader/meta.yml index 60704ab4..690d4ead 100644 --- a/modules/nf-core/bcftools/reheader/meta.yml +++ b/modules/nf-core/bcftools/reheader/meta.yml @@ -12,7 +12,6 @@ tools: documentation: http://samtools.github.io/bcftools/bcftools.html#reheader doi: 10.1093/gigascience/giab008 licence: ["MIT"] - input: - meta: type: map @@ -27,6 +26,10 @@ input: type: file description: New header to add to the VCF pattern: "*.{header.txt}" + - samples: + type: file + description: File containing sample names to update (one sample per line) + pattern: "*.{samples.txt}" - meta2: type: map description: | @@ -36,7 +39,6 @@ input: type: file description: Fasta index to update header sequences with pattern: "*.{fai}" - output: - meta: type: map @@ -51,8 +53,11 @@ output: type: file description: VCF with updated header, bgzipped per default pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}" - authors: - "@bjohnnyd" - "@jemten" - "@ramprasadn" +maintainers: + - "@bjohnnyd" + - "@jemten" + - "@ramprasadn" diff --git a/modules/nf-core/bcftools/roh/main.nf b/modules/nf-core/bcftools/roh/main.nf index d8a8bc79..8e03e47d 100644 --- a/modules/nf-core/bcftools/roh/main.nf +++ b/modules/nf-core/bcftools/roh/main.nf @@ -2,10 +2,10 @@ process BCFTOOLS_ROH { tag "$meta.id" label 'process_medium' - conda "bioconda::bcftools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': - 'biocontainers/bcftools:1.17--haef29d1_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'biocontainers/bcftools:1.18--h8b25389_0' }" input: tuple val(meta), path(vcf), path(tbi) diff --git a/modules/nf-core/bcftools/roh/meta.yml b/modules/nf-core/bcftools/roh/meta.yml index fa0fd088..ee928a8f 100644 --- a/modules/nf-core/bcftools/roh/meta.yml +++ b/modules/nf-core/bcftools/roh/meta.yml @@ -2,6 +2,9 @@ name: "bcftools_roh" description: A program for detecting runs of homo/autozygosity. Only bi-allelic sites are considered. keywords: - roh + - biallelic + - homozygosity + - autozygosity tools: - "roh": description: "A program for detecting runs of homo/autozygosity. Only bi-allelic sites are considered." @@ -9,7 +12,6 @@ tools: documentation: http://www.htslib.org/doc/bcftools.html doi: 10.1093/bioinformatics/btp352 licence: ["MIT"] - input: - meta: type: map @@ -38,7 +40,6 @@ input: - targets_file: type: file description: "Targets can be specified either on command line or in a VCF, BED, or tab-delimited file (the default)." - output: - meta: type: map @@ -53,6 +54,7 @@ output: type: file description: Contains site-specific and/or per-region runs of homo/autozygosity calls. pattern: "*.{roh}" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/bcftools/sort/main.nf b/modules/nf-core/bcftools/sort/main.nf index ef41fd25..c982944c 100644 --- a/modules/nf-core/bcftools/sort/main.nf +++ b/modules/nf-core/bcftools/sort/main.nf @@ -2,10 +2,10 @@ process BCFTOOLS_SORT { tag "$meta.id" label 'process_medium' - conda "bioconda::bcftools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': - 'biocontainers/bcftools:1.17--haef29d1_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'biocontainers/bcftools:1.18--h8b25389_0' }" input: tuple val(meta), path(vcf) @@ -30,6 +30,7 @@ process BCFTOOLS_SORT { bcftools \\ sort \\ --output ${prefix}.${extension} \\ + --temp-dir . \\ $args \\ $vcf diff --git a/modules/nf-core/bcftools/sort/meta.yml b/modules/nf-core/bcftools/sort/meta.yml index 0c244a48..84747c6d 100644 --- a/modules/nf-core/bcftools/sort/meta.yml +++ b/modules/nf-core/bcftools/sort/meta.yml @@ -12,7 +12,6 @@ tools: tool_dev_url: https://github.com/samtools/bcftools doi: "10.1093/bioinformatics/btp352" licence: ["MIT"] - input: - meta: type: map @@ -23,7 +22,6 @@ input: type: file description: The VCF/BCF file to be sorted pattern: "*.{vcf.gz,vcf,bcf}" - output: - meta: type: map @@ -38,6 +36,7 @@ output: type: file description: Sorted VCF file pattern: "*.{vcf.gz}" - authors: - "@Gwennid" +maintainers: + - "@Gwennid" diff --git a/modules/nf-core/bcftools/view/main.nf b/modules/nf-core/bcftools/view/main.nf index 86f807d3..a9d93fa6 100644 --- a/modules/nf-core/bcftools/view/main.nf +++ b/modules/nf-core/bcftools/view/main.nf @@ -2,10 +2,10 @@ process BCFTOOLS_VIEW { tag "$meta.id" label 'process_medium' - conda "bioconda::bcftools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': - 'biocontainers/bcftools:1.17--haef29d1_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'biocontainers/bcftools:1.18--h8b25389_0' }" input: tuple val(meta), path(vcf), path(index) diff --git a/modules/nf-core/bcftools/view/meta.yml b/modules/nf-core/bcftools/view/meta.yml index 326fd1fa..eaa12b56 100644 --- a/modules/nf-core/bcftools/view/meta.yml +++ b/modules/nf-core/bcftools/view/meta.yml @@ -5,7 +5,6 @@ keywords: - view - bcftools - VCF - tools: - view: description: | @@ -61,3 +60,5 @@ output: pattern: "versions.yml" authors: - "@abhi18av" +maintainers: + - "@abhi18av" diff --git a/modules/nf-core/bwa/index/main.nf b/modules/nf-core/bwa/index/main.nf index 8d2e56d9..24b5a2ea 100644 --- a/modules/nf-core/bwa/index/main.nf +++ b/modules/nf-core/bwa/index/main.nf @@ -2,7 +2,7 @@ process BWA_INDEX { tag "$fasta" label 'process_single' - conda "bioconda::bwa=0.7.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bwa:0.7.17--hed695b0_7' : 'biocontainers/bwa:0.7.17--hed695b0_7' }" @@ -18,13 +18,14 @@ process BWA_INDEX { task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${fasta.baseName}" + def args = task.ext.args ?: '' """ mkdir bwa bwa \\ index \\ $args \\ - -p bwa/${fasta.baseName} \\ + -p bwa/${prefix} \\ $fasta cat <<-END_VERSIONS > versions.yml @@ -34,14 +35,15 @@ process BWA_INDEX { """ stub: + def prefix = task.ext.prefix ?: "${fasta.baseName}" """ mkdir bwa - touch bwa/genome.amb - touch bwa/genome.ann - touch bwa/genome.bwt - touch bwa/genome.pac - touch bwa/genome.sa + touch bwa/${prefix}.amb + touch bwa/${prefix}.ann + touch bwa/${prefix}.bwt + touch bwa/${prefix}.pac + touch bwa/${prefix}.sa cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/bwa/index/meta.yml b/modules/nf-core/bwa/index/meta.yml index 2c6cfcd7..730628d0 100644 --- a/modules/nf-core/bwa/index/meta.yml +++ b/modules/nf-core/bwa/index/meta.yml @@ -40,3 +40,6 @@ output: authors: - "@drpatelh" - "@maxulysse" +maintainers: + - "@drpatelh" + - "@maxulysse" diff --git a/modules/nf-core/bwamem2/index/main.nf b/modules/nf-core/bwamem2/index/main.nf index 30940852..b7688285 100644 --- a/modules/nf-core/bwamem2/index/main.nf +++ b/modules/nf-core/bwamem2/index/main.nf @@ -2,7 +2,7 @@ process BWAMEM2_INDEX { tag "$fasta" label 'process_single' - conda "bioconda::bwa-mem2=2.2.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bwa-mem2:2.2.1--he513fc3_0' : 'biocontainers/bwa-mem2:2.2.1--he513fc3_0' }" @@ -18,13 +18,14 @@ process BWAMEM2_INDEX { task.ext.when == null || task.ext.when script: + def prefix = task.ext.prefix ?: "${fasta}" def args = task.ext.args ?: '' """ mkdir bwamem2 bwa-mem2 \\ index \\ $args \\ - $fasta -p bwamem2/${fasta} + $fasta -p bwamem2/${prefix} cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -33,13 +34,15 @@ process BWAMEM2_INDEX { """ stub: + def prefix = task.ext.prefix ?: "${fasta}" + """ mkdir bwamem2 - touch bwamem2/${fasta}.0123 - touch bwamem2/${fasta}.ann - touch bwamem2/${fasta}.pac - touch bwamem2/${fasta}.amb - touch bwamem2/${fasta}.bwt.2bit.64 + touch bwamem2/${prefix}.0123 + touch bwamem2/${prefix}.ann + touch bwamem2/${prefix}.pac + touch bwamem2/${prefix}.amb + touch bwamem2/${prefix}.bwt.2bit.64 cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/bwamem2/index/meta.yml b/modules/nf-core/bwamem2/index/meta.yml index 40c26c38..c14a1092 100644 --- a/modules/nf-core/bwamem2/index/meta.yml +++ b/modules/nf-core/bwamem2/index/meta.yml @@ -38,3 +38,5 @@ output: pattern: "versions.yml" authors: - "@maxulysse" +maintainers: + - "@maxulysse" diff --git a/modules/nf-core/bwamem2/mem/main.nf b/modules/nf-core/bwamem2/mem/main.nf index d427dea3..b3874fc9 100644 --- a/modules/nf-core/bwamem2/mem/main.nf +++ b/modules/nf-core/bwamem2/mem/main.nf @@ -2,10 +2,10 @@ process BWAMEM2_MEM { tag "$meta.id" label 'process_high' - conda "bioconda::bwa-mem2=2.2.1 bioconda::samtools=1.16.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:2cdf6bf1e92acbeb9b2834b1c58754167173a410-0' : - 'biocontainers/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:2cdf6bf1e92acbeb9b2834b1c58754167173a410-0' }" + 'https://depot.galaxyproject.org/singularity/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:6351200f24497efba12c219c2bea4bb0f69a9d47-0' : + 'biocontainers/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:6351200f24497efba12c219c2bea4bb0f69a9d47-0' }" input: tuple val(meta), path(reads) diff --git a/modules/nf-core/bwamem2/mem/meta.yml b/modules/nf-core/bwamem2/mem/meta.yml index bc3dfcdd..04891b26 100644 --- a/modules/nf-core/bwamem2/mem/meta.yml +++ b/modules/nf-core/bwamem2/mem/meta.yml @@ -57,3 +57,5 @@ output: pattern: "versions.yml" authors: - "@maxulysse" +maintainers: + - "@maxulysse" diff --git a/modules/nf-core/cadd/main.nf b/modules/nf-core/cadd/main.nf index 0f644811..d4fab1f4 100644 --- a/modules/nf-core/cadd/main.nf +++ b/modules/nf-core/cadd/main.nf @@ -2,7 +2,7 @@ process CADD { tag "$meta.id" label 'process_medium' - conda "bioconda::cadd-scripts=1.6 anaconda::conda=4.14.0 conda-forge::mamba=1.4.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-8d145e7b16a8ca4bf920e6ca464763df6f0a56a2:d4e457a2edecb2b10e915c01d8f46e29e236b648-0': 'biocontainers/mulled-v2-8d145e7b16a8ca4bf920e6ca464763df6f0a56a2:d4e457a2edecb2b10e915c01d8f46e29e236b648-0' }" diff --git a/modules/nf-core/cadd/meta.yml b/modules/nf-core/cadd/meta.yml index b54f5951..df84d237 100644 --- a/modules/nf-core/cadd/meta.yml +++ b/modules/nf-core/cadd/meta.yml @@ -12,7 +12,6 @@ tools: tool_dev_url: "https://github.com/kircherlab/CADD-scripts/" doi: "10.1093/nar/gky1016" licence: "['Restricted. Free for non-commercial users.']" - input: - meta: type: map @@ -29,7 +28,6 @@ input: Path to folder containing the vcf files with precomputed CADD scores. This folder contains the uncompressed files that would otherwise be in data/annotation folder as described in https://github.com/kircherlab/CADD-scripts/#manual-installation. pattern: "*.{vcf,vcf.gz}" - output: - meta: type: map @@ -44,6 +42,7 @@ output: type: file description: Annotated tsv file pattern: "*.{tsv,tsv.gz}" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/cat/cat/main.nf b/modules/nf-core/cat/cat/main.nf index 970ab760..adbdbd7b 100644 --- a/modules/nf-core/cat/cat/main.nf +++ b/modules/nf-core/cat/cat/main.nf @@ -22,6 +22,8 @@ process CAT_CAT { def args2 = task.ext.args2 ?: '' def file_list = files_in.collect { it.toString() } + // choose appropriate concatenation tool depending on input and output format + // | input | output | command1 | command2 | // |-----------|------------|----------|----------| // | gzipped | gzipped | cat | | @@ -30,7 +32,7 @@ process CAT_CAT { // | ungzipped | gzipped | cat | pigz | // Use input file ending as default - prefix = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}" + prefix = task.ext.prefix ?: "${meta.id}${getFileSuffix(file_list[0])}" out_zip = prefix.endsWith('.gz') in_zip = file_list[0].endsWith('.gz') command1 = (in_zip && !out_zip) ? 'zcat' : 'cat' @@ -68,3 +70,10 @@ process CAT_CAT { END_VERSIONS """ } + +// for .gz files also include the second to last extension if it is present. E.g., .fasta.gz +def getFileSuffix(filename) { + def match = filename =~ /^.*?((\.\w{1,5})?(\.\w{1,5}\.gz$))/ + return match ? match[0][1] : filename.substring(filename.lastIndexOf('.')) +} + diff --git a/modules/nf-core/cat/cat/tests/main.nf.test b/modules/nf-core/cat/cat/tests/main.nf.test index ed5a4f12..aaae04f9 100644 --- a/modules/nf-core/cat/cat/tests/main.nf.test +++ b/modules/nf-core/cat/cat/tests/main.nf.test @@ -83,8 +83,7 @@ nextflow_process { def lines = path(process.out.file_out.get(0).get(1)).linesGzip assertAll( { assert process.success }, - { assert snapshot(lines[0..5]).match("test_cat_zipped_zipped_lines") }, - { assert snapshot(lines.size()).match("test_cat_zipped_zipped_size")} + { assert snapshot(process.out).match() } ) } } @@ -142,8 +141,7 @@ nextflow_process { def lines = path(process.out.file_out.get(0).get(1)).linesGzip assertAll( { assert process.success }, - { assert snapshot(lines[0..5]).match("test_cat_unzipped_zipped_lines") }, - { assert snapshot(lines.size()).match("test_cat_unzipped_zipped_size")} + { assert snapshot(process.out).match() } ) } } diff --git a/modules/nf-core/cat/cat/tests/main.nf.test.snap b/modules/nf-core/cat/cat/tests/main.nf.test.snap index 423571ba..0c9bfe8d 100644 --- a/modules/nf-core/cat/cat/tests/main.nf.test.snap +++ b/modules/nf-core/cat/cat/tests/main.nf.test.snap @@ -1,10 +1,4 @@ { - "test_cat_unzipped_zipped_size": { - "content": [ - 375 - ], - "timestamp": "2023-10-16T14:33:08.049445686" - }, "test_cat_unzipped_unzipped": { "content": [ { @@ -67,31 +61,36 @@ ], "timestamp": "2023-10-16T14:32:49.642741302" }, - "test_cat_zipped_zipped_lines": { - "content": [ - [ - "MT192765.1\tGenbank\ttranscript\t259\t29667\t.\t+\t.\tID=unknown_transcript_1;geneID=orf1ab;gene_name=orf1ab", - "MT192765.1\tGenbank\tgene\t259\t21548\t.\t+\t.\tParent=unknown_transcript_1", - "MT192765.1\tGenbank\tCDS\t259\t13461\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1", - "MT192765.1\tGenbank\tCDS\t13461\t21548\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1", - "MT192765.1\tGenbank\tCDS\t21556\t25377\t.\t+\t0\tParent=unknown_transcript_1;gbkey=CDS;gene=S;note=\"structural protein\";product=\"surface glycoprotein\";protein_id=QIK50427.1", - "MT192765.1\tGenbank\tgene\t21556\t25377\t.\t+\t.\tParent=unknown_transcript_1" - ] - ], - "timestamp": "2023-10-16T14:32:33.629048645" - }, - "test_cat_unzipped_zipped_lines": { + "test_cat_zipped_zipped": { "content": [ - [ - ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome", - "GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGT", - "GTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAG", - "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG", - "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT", - "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG" - ] + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.gff3.gz:md5,c439d3b60e7bc03e8802a451a0d9a5d9" + ] + ], + "1": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ], + "file_out": [ + [ + { + "id": "test", + "single_end": true + }, + "test.gff3.gz:md5,c439d3b60e7bc03e8802a451a0d9a5d9" + ] + ], + "versions": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ] + } ], - "timestamp": "2023-10-16T14:33:08.038830506" + "timestamp": "2024-01-12T14:02:02.999254641" }, "test_cat_one_file_unzipped_zipped_lines": { "content": [ @@ -106,16 +105,41 @@ ], "timestamp": "2023-10-16T14:33:21.39642399" }, - "test_cat_zipped_zipped_size": { + "test_cat_unzipped_zipped": { "content": [ - 78 + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "cat.txt.gz:md5,f44b33a0e441ad58b2d3700270e2dbe2" + ] + ], + "1": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ], + "file_out": [ + [ + { + "id": "test", + "single_end": true + }, + "cat.txt.gz:md5,f44b33a0e441ad58b2d3700270e2dbe2" + ] + ], + "versions": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ] + } ], - "timestamp": "2023-10-16T14:32:33.641869244" + "timestamp": "2024-01-12T14:08:26.948048418" }, "test_cat_one_file_unzipped_zipped_size": { "content": [ 374 ], - "timestamp": "2023-10-16T14:33:21.4094373" + "timestamp": "2024-01-12T14:10:22.445700266" } -} \ No newline at end of file +} diff --git a/modules/nf-core/chromograph/main.nf b/modules/nf-core/chromograph/main.nf index 9049dbfa..dd67e1ba 100644 --- a/modules/nf-core/chromograph/main.nf +++ b/modules/nf-core/chromograph/main.nf @@ -2,7 +2,7 @@ process CHROMOGRAPH { tag "$meta.id" label 'process_single' - conda "bioconda::chromograph=1.3.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/chromograph:1.3.1--pyhdfd78af_1': 'biocontainers/chromograph:1.3.1--pyhdfd78af_1' }" diff --git a/modules/nf-core/chromograph/meta.yml b/modules/nf-core/chromograph/meta.yml index cac5c7aa..0fd7250f 100644 --- a/modules/nf-core/chromograph/meta.yml +++ b/modules/nf-core/chromograph/meta.yml @@ -13,7 +13,6 @@ tools: homepage: "https://github.com/Clinical-Genomics/chromograph" documentation: "https://github.com/Clinical-Genomics/chromograph/blob/master/README.md" licence: "['MIT']" - input: - meta: type: map @@ -73,7 +72,6 @@ input: - sites: type: file description: Bed file containing UPD sites - output: - meta: type: map @@ -88,6 +86,7 @@ output: type: file description: Directory containing the plots in png format pattern: "*.png" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/cnvnator/cnvnator/main.nf b/modules/nf-core/cnvnator/cnvnator/main.nf index 6eb8a4f0..07393639 100644 --- a/modules/nf-core/cnvnator/cnvnator/main.nf +++ b/modules/nf-core/cnvnator/cnvnator/main.nf @@ -2,7 +2,7 @@ process CNVNATOR_CNVNATOR { tag "$meta.id" label 'process_single' - conda "bioconda::cnvnator=0.4.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/cnvnator:0.4.1--py310h2dce045_7': 'biocontainers/cnvnator:0.4.1--py310h2dce045_7' }" diff --git a/modules/nf-core/cnvnator/cnvnator/meta.yml b/modules/nf-core/cnvnator/cnvnator/meta.yml index ea4f09be..2afbacde 100644 --- a/modules/nf-core/cnvnator/cnvnator/meta.yml +++ b/modules/nf-core/cnvnator/cnvnator/meta.yml @@ -11,7 +11,6 @@ tools: documentation: "https://github.com/abyzovlab/CNVnator/blob/master/README.md" tool_dev_url: "https://github.com/abyzovlab/CNVnator" licence: "['MIT']" - input: - meta: type: map @@ -53,7 +52,6 @@ input: type: file description: Path to a fasta file index pattern: "*.fai" - output: - output_meta: type: map @@ -72,6 +70,7 @@ output: type: file description: A tab file containing cnvnator calls pattern: "*.tab" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/cnvnator/convert2vcf/main.nf b/modules/nf-core/cnvnator/convert2vcf/main.nf index 1ca05ab0..87dd031a 100644 --- a/modules/nf-core/cnvnator/convert2vcf/main.nf +++ b/modules/nf-core/cnvnator/convert2vcf/main.nf @@ -2,7 +2,7 @@ process CNVNATOR_CONVERT2VCF { tag "$meta.id" label 'process_single' - conda "bioconda::cnvnator=0.4.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/cnvnator:0.4.1--py310h2dce045_7': 'biocontainers/cnvnator:0.4.1--py310h2dce045_7' }" diff --git a/modules/nf-core/cnvnator/convert2vcf/meta.yml b/modules/nf-core/cnvnator/convert2vcf/meta.yml index 5df7bc65..8e8c8006 100644 --- a/modules/nf-core/cnvnator/convert2vcf/meta.yml +++ b/modules/nf-core/cnvnator/convert2vcf/meta.yml @@ -11,7 +11,6 @@ tools: documentation: "https://github.com/abyzovlab/CNVnator/blob/master/README.md" tool_dev_url: "https://github.com/abyzovlab/CNVnator" licence: "['MIT']" - input: - meta: type: map @@ -22,7 +21,6 @@ input: type: file description: A tab file containing CNVnator calls pattern: "*.tab" - output: - meta: type: map @@ -37,6 +35,7 @@ output: type: file description: CNVnator calls in vcf format pattern: "*.vcf" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index f0c63f69..9b3272bc 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.17 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 7685b33c..f2187611 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test index eec1db10..b1e1630b 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -31,7 +31,12 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } ) } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap index 4274ed57..5f59a936 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -1,27 +1,33 @@ { "Should run without failures": { "content": [ - { - "0": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ], - "1": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "2": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "mqc_yml": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "versions": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "yml": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ] - } + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] ], - "timestamp": "2023-11-03T14:43:22.157011" + "timestamp": "2024-01-09T23:01:18.710682" } -} +} \ No newline at end of file diff --git a/modules/nf-core/deepvariant/meta.yml b/modules/nf-core/deepvariant/meta.yml index c7d11ae3..a50dc57d 100644 --- a/modules/nf-core/deepvariant/meta.yml +++ b/modules/nf-core/deepvariant/meta.yml @@ -12,7 +12,6 @@ tools: tool_dev_url: https://github.com/google/deepvariant doi: "10.1038/nbt.4235" licence: ["BSD-3-clause"] - input: - meta: type: map @@ -58,7 +57,6 @@ input: type: file description: GZI index of reference fasta file pattern: "*.gzi" - output: - meta: type: map @@ -77,7 +75,9 @@ output: type: file description: File containing software version pattern: "*.{version.txt}" - authors: - "@abhi18av" - "@ramprasadn" +maintainers: + - "@abhi18av" + - "@ramprasadn" diff --git a/modules/nf-core/ensemblvep/filtervep/main.nf b/modules/nf-core/ensemblvep/filtervep/main.nf index 7fa2477a..53abf772 100644 --- a/modules/nf-core/ensemblvep/filtervep/main.nf +++ b/modules/nf-core/ensemblvep/filtervep/main.nf @@ -2,7 +2,7 @@ process ENSEMBLVEP_FILTERVEP { tag "$meta.id" label 'process_single' - conda "bioconda::ensembl-vep=110.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ensembl-vep:110.0--pl5321h2a3209d_0' : 'biocontainers/ensembl-vep:110.0--pl5321h2a3209d_0' }" diff --git a/modules/nf-core/ensemblvep/filtervep/meta.yml b/modules/nf-core/ensemblvep/filtervep/meta.yml index 603c0040..bde3aa16 100644 --- a/modules/nf-core/ensemblvep/filtervep/meta.yml +++ b/modules/nf-core/ensemblvep/filtervep/meta.yml @@ -1,4 +1,4 @@ -name: "ensemblvep_filtervep" +name: ensemblvep_filtervep description: Filter variants based on Ensembl Variant Effect Predictor (VEP) annotations. keywords: - annotation @@ -13,7 +13,6 @@ tools: homepage: https://www.ensembl.org/info/docs/tools/vep/index.html documentation: https://www.ensembl.org/info/docs/tools/vep/script/index.html licence: ["Apache-2.0"] - input: - meta: type: map @@ -27,7 +26,6 @@ input: - feature_file: type: file description: File containing features on separate lines. To be used with --filter option. - output: - meta: type: map @@ -42,6 +40,7 @@ output: type: file description: VCF/TAB file pattern: "*.{vcf,tab,txt,tsv}" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/expansionhunter/main.nf b/modules/nf-core/expansionhunter/main.nf index b5339bf6..008b13ad 100644 --- a/modules/nf-core/expansionhunter/main.nf +++ b/modules/nf-core/expansionhunter/main.nf @@ -2,10 +2,10 @@ process EXPANSIONHUNTER { tag "$meta.id" label 'process_low' - conda "bioconda::expansionhunter=4.0.2" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/expansionhunter:4.0.2--he785bd8_0' : - 'biocontainers/expansionhunter:4.0.2--he785bd8_0' }" + 'https://depot.galaxyproject.org/singularity/expansionhunter:5.0.0--hf366f20_0' : + 'biocontainers/expansionhunter:5.0.0--hf366f20_0' }" input: tuple val(meta), path(bam), path(bai) diff --git a/modules/nf-core/expansionhunter/meta.yml b/modules/nf-core/expansionhunter/meta.yml index 0d2b10d6..b970ced4 100644 --- a/modules/nf-core/expansionhunter/meta.yml +++ b/modules/nf-core/expansionhunter/meta.yml @@ -14,7 +14,6 @@ tools: documentation: https://github.com/Illumina/ExpansionHunter/blob/master/docs/01_Introduction.md doi: "10.1093/bioinformatics/btz431" licence: ["Apache-2.0"] - input: - meta: type: map @@ -52,7 +51,6 @@ input: type: file description: JSON file with repeat expansion sites to genotype pattern: "*.json" - output: - meta: type: map @@ -71,6 +69,7 @@ output: type: file description: JSON with repeat expansions pattern: "*.json.gz" - authors: - "@jemten" +maintainers: + - "@jemten" diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf index 5fac3c1a..2a3b679e 100644 --- a/modules/nf-core/fastp/main.nf +++ b/modules/nf-core/fastp/main.nf @@ -99,4 +99,22 @@ process FASTP { END_VERSIONS """ } + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def is_single_output = task.ext.args?.contains('--interleaved_in') || meta.single_end + def touch_reads = is_single_output ? "${prefix}.fastp.fastq.gz" : "${prefix}_1.fastp.fastq.gz ${prefix}_2.fastp.fastq.gz" + def touch_merged = (!is_single_output && save_merged) ? "touch ${prefix}.merged.fastq.gz" : "" + """ + touch $touch_reads + touch "${prefix}.fastp.json" + touch "${prefix}.fastp.html" + touch "${prefix}.fastp.log" + $touch_merged + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") + END_VERSIONS + """ } diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test index f610b735..fa7e5b4d 100644 --- a/modules/nf-core/fastp/tests/main.nf.test +++ b/modules/nf-core/fastp/tests/main.nf.test @@ -19,11 +19,10 @@ nextflow_process { save_trimmed_fail = false save_merged = false - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:true ], - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] - ] - + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -57,6 +56,66 @@ nextflow_process { { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } } }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_single_end-for_stub_match") + }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("test_fastp_single_end-stub") { + + options '-stub' + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + + assertAll( + { assert process.success }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_single_end-for_stub_match") + }, { assert snapshot(process.out.versions).match("versions") } ) } @@ -74,12 +133,11 @@ nextflow_process { save_trimmed_fail = false save_merged = false - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] - ] - + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -127,6 +185,66 @@ nextflow_process { { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } } }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_paired_end-for_stub_match") + }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("test_fastp_paired_end-stub") { + + options '-stub' + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + assertAll( + { assert process.success }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_paired_end-for_stub_match") + }, { assert snapshot(process.out.versions).match("versions") } ) } @@ -144,10 +262,10 @@ nextflow_process { save_trimmed_fail = false save_merged = false - input[0] = [ [ id:'test', single_end:true ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) ] - ] - + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -181,6 +299,66 @@ nextflow_process { { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } } }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_interleaved-for_stub_match") + }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("fastp test_fastp_interleaved-stub") { + + options '-stub' + + config './nextflow.config' + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + assertAll( + { assert process.success }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_interleaved-for_stub_match") + }, { assert snapshot(process.out.versions).match("versions") } ) } @@ -198,9 +376,10 @@ nextflow_process { save_trimmed_fail = true save_merged = false - input[0] = [ [ id:'test', single_end:true ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] - ] + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -258,13 +437,11 @@ nextflow_process { save_trimmed_fail = true save_merged = false - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) - ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -337,11 +514,11 @@ nextflow_process { adapter_fasta = [] save_trimmed_fail = false save_merged = true - - input[0] = [ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -399,6 +576,66 @@ nextflow_process { { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } } }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_paired_end_merged-for_stub_match") + }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("test_fastp_paired_end_merged-stub") { + + options '-stub' + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = true + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + assertAll( + { assert process.success }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_paired_end_merged-for_stub_match") + }, { assert snapshot(process.out.versions).match("versions") } ) } @@ -412,14 +649,15 @@ nextflow_process { } process { """ - adapter_fasta = file("https://github.com/nf-core/test-datasets/raw/modules/data/delete_me/fastp/adapters.fasta", checkIfExists: true) + adapter_fasta = Channel.of([ file(params.modules_testdata_base_path + 'delete_me/fastp/adapters.fasta', checkIfExists: true) ]) save_trimmed_fail = false save_merged = true - input[0] = [ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap index 0fa68c7d..6a71b680 100644 --- a/modules/nf-core/fastp/tests/main.nf.test.snap +++ b/modules/nf-core/fastp/tests/main.nf.test.snap @@ -1,4 +1,19 @@ { + "test_fastp_paired_end-for_stub_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=false}" + ] + ], + "timestamp": "2024-01-17T18:07:15.398827" + }, "fastp test_fastp_interleaved_json": { "content": [ [ @@ -11,7 +26,23 @@ ] ] ], - "timestamp": "2023-10-17T11:04:45.794175881" + "timestamp": "2024-01-17T18:08:06.123035" + }, + "test_fastp_paired_end_merged-for_stub_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "test.merged.fastq.gz", + "{id=test, single_end=false}" + ] + ], + "timestamp": "2024-01-17T18:10:13.467574" }, "test_fastp_single_end_json": { "content": [ @@ -25,7 +56,7 @@ ] ] ], - "timestamp": "2023-10-17T11:04:10.566343705" + "timestamp": "2024-01-17T18:06:00.223817" }, "versions": { "content": [ @@ -33,7 +64,31 @@ "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" ] ], - "timestamp": "2023-10-17T11:04:10.582076024" + "timestamp": "2024-01-17T18:06:00.248422" + }, + "test_fastp_interleaved-for_stub_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "timestamp": "2024-01-17T18:08:06.127974" + }, + "test_fastp_single_end-for_stub_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "timestamp": "2024-01-17T18:06:00.244202" }, "test_fastp_single_end_trim_fail_json": { "content": [ @@ -47,6 +102,6 @@ ] ] ], - "timestamp": "2023-10-17T11:05:00.379878948" + "timestamp": "2024-01-17T18:08:41.942317" } } \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index b9e8f926..1f21c664 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -3,24 +3,20 @@ nextflow_process { name "Test Process FASTQC" script "../main.nf" process "FASTQC" + tag "modules" tag "modules_nfcore" tag "fastqc" - test("Single-Read") { + test("sarscov2 single-end [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ - input[0] = [ + input[0] = Channel.of([ [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) """ } } @@ -28,82 +24,189 @@ nextflow_process { then { assertAll ( { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. // looks like this:
Mon 2 Oct 2023
test.gz
// https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, - { assert path(process.out.html.get(0).get(1)).getText().contains("") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 paired-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 interleaved [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } ) } } -// TODO -// // -// // Test with paired-end data -// // -// workflow test_fastqc_paired_end { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with interleaved data -// // -// workflow test_fastqc_interleaved { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with bam data -// // -// workflow test_fastqc_bam { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with multiple samples -// // -// workflow test_fastqc_multiple { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with custom prefix -// // -// workflow test_fastqc_custom_prefix { -// input = [ -// [ id:'mysample', single_end:true ], // meta map -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } + + test("sarscov2 paired-end [bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 multiple [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + { assert path(process.out.html[0][1][2]).text.contains("") }, + { assert path(process.out.html[0][1][3]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 custom_prefix") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastq] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.html.collect { file(it[1]).getName() } + + process.out.zip.collect { file(it[1]).getName() } + + process.out.versions ).match() } + ) + } + } + } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 636a32ce..5d624bb8 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,10 +1,20 @@ { + "sarscov2 single-end [fastq] - stub": { + "content": [ + [ + "test.html", + "test.zip", + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2024-01-17T18:40:57.254299" + }, "versions": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2023-10-09T23:40:54+0000" + "timestamp": "2024-01-17T18:36:50.033627" } } \ No newline at end of file diff --git a/modules/nf-core/gatk4/bedtointervallist/main.nf b/modules/nf-core/gatk4/bedtointervallist/main.nf index a23abd06..88b24b1a 100644 --- a/modules/nf-core/gatk4/bedtointervallist/main.nf +++ b/modules/nf-core/gatk4/bedtointervallist/main.nf @@ -2,7 +2,7 @@ process GATK4_BEDTOINTERVALLIST { tag "$meta.id" label 'process_medium' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -29,7 +29,8 @@ process GATK4_BEDTOINTERVALLIST { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" BedToIntervalList \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + BedToIntervalList \\ --INPUT $bed \\ --OUTPUT ${prefix}.interval_list \\ --SEQUENCE_DICTIONARY $dict \\ diff --git a/modules/nf-core/gatk4/bedtointervallist/meta.yml b/modules/nf-core/gatk4/bedtointervallist/meta.yml index 40daf752..187da885 100644 --- a/modules/nf-core/gatk4/bedtointervallist/meta.yml +++ b/modules/nf-core/gatk4/bedtointervallist/meta.yml @@ -2,8 +2,9 @@ name: gatk4_bedtointervallist description: Creates an interval list from a bed file and a reference dict keywords: - bed - - interval list - bedtointervallist + - gatk4 + - interval list tools: - gatk4: description: | @@ -45,3 +46,6 @@ output: authors: - "@kevinmenden" - "@ramprasadn" +maintainers: + - "@kevinmenden" + - "@ramprasadn" diff --git a/modules/nf-core/gatk4/collectreadcounts/main.nf b/modules/nf-core/gatk4/collectreadcounts/main.nf index ce1985bc..1cc36941 100644 --- a/modules/nf-core/gatk4/collectreadcounts/main.nf +++ b/modules/nf-core/gatk4/collectreadcounts/main.nf @@ -2,7 +2,7 @@ process GATK4_COLLECTREADCOUNTS { tag "$meta.id" label 'process_medium' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -37,7 +37,8 @@ process GATK4_COLLECTREADCOUNTS { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" CollectReadCounts \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + CollectReadCounts \\ --input $input \\ --intervals $intervals \\ --output ${prefix}.$extension \\ diff --git a/modules/nf-core/gatk4/collectreadcounts/meta.yml b/modules/nf-core/gatk4/collectreadcounts/meta.yml index 938011c1..25fb8b8c 100644 --- a/modules/nf-core/gatk4/collectreadcounts/meta.yml +++ b/modules/nf-core/gatk4/collectreadcounts/meta.yml @@ -1,23 +1,18 @@ name: "gatk4_collectreadcounts" description: Collects read counts at specified intervals. The count for each interval is calculated by counting the number of read starts that lie in the interval. keywords: + - collectreadcounts - bam - cram - - CollectReadCounts - - gatk - gatk4 tools: - gatk4: - description: - Genome Analysis Toolkit (GATK4). Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. + description: Genome Analysis Toolkit (GATK4). Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. homepage: https://gatk.broadinstitute.org/hc/en-us documentation: https://gatk.broadinstitute.org/hc/en-us/articles/360037593911-CombineGVCFs tool_dev_url: https://github.com/broadinstitute/gatk doi: 10.1158/1538-7445.AM2017-3590 licence: ["Apache-2.0"] - input: - meta: type: map @@ -63,7 +58,6 @@ input: type: file description: Optional - Sequence dictionary of the reference FASTA file pattern: "*.dict" - output: - meta: type: map @@ -82,6 +76,7 @@ output: type: file description: The read counts in TSV format pattern: "*.tsv" - authors: - "@nvnieuwk" +maintainers: + - "@nvnieuwk" diff --git a/modules/nf-core/gatk4/createsequencedictionary/main.nf b/modules/nf-core/gatk4/createsequencedictionary/main.nf index 15a86bea..b47ad162 100644 --- a/modules/nf-core/gatk4/createsequencedictionary/main.nf +++ b/modules/nf-core/gatk4/createsequencedictionary/main.nf @@ -2,7 +2,7 @@ process GATK4_CREATESEQUENCEDICTIONARY { tag "$fasta" label 'process_medium' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -27,7 +27,8 @@ process GATK4_CREATESEQUENCEDICTIONARY { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" CreateSequenceDictionary \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + CreateSequenceDictionary \\ --REFERENCE $fasta \\ --URI $fasta \\ --TMP_DIR . \\ diff --git a/modules/nf-core/gatk4/createsequencedictionary/meta.yml b/modules/nf-core/gatk4/createsequencedictionary/meta.yml index a421e681..f9d70be0 100644 --- a/modules/nf-core/gatk4/createsequencedictionary/meta.yml +++ b/modules/nf-core/gatk4/createsequencedictionary/meta.yml @@ -1,9 +1,10 @@ name: gatk4_createsequencedictionary description: Creates a sequence dictionary for a reference sequence keywords: + - createsequencedictionary - dictionary - fasta - - createsequencedictionary + - gatk4 tools: - gatk: description: | @@ -14,7 +15,6 @@ tools: documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s doi: 10.1158/1538-7445.AM2017-3590 licence: ["Apache-2.0"] - input: - meta: type: map @@ -37,3 +37,6 @@ output: authors: - "@maxulysse" - "@ramprasadn" +maintainers: + - "@maxulysse" + - "@ramprasadn" diff --git a/modules/nf-core/gatk4/denoisereadcounts/main.nf b/modules/nf-core/gatk4/denoisereadcounts/main.nf index e60aeda1..b1191c27 100644 --- a/modules/nf-core/gatk4/denoisereadcounts/main.nf +++ b/modules/nf-core/gatk4/denoisereadcounts/main.nf @@ -2,7 +2,7 @@ process GATK4_DENOISEREADCOUNTS { tag "$meta.id" label 'process_single' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -30,7 +30,8 @@ process GATK4_DENOISEREADCOUNTS { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" DenoiseReadCounts \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + DenoiseReadCounts \\ ${args} \\ --tmp-dir . \\ --input ${counts} \\ diff --git a/modules/nf-core/gatk4/denoisereadcounts/meta.yml b/modules/nf-core/gatk4/denoisereadcounts/meta.yml index 0bb2f389..f2bd853f 100644 --- a/modules/nf-core/gatk4/denoisereadcounts/meta.yml +++ b/modules/nf-core/gatk4/denoisereadcounts/meta.yml @@ -1,9 +1,9 @@ name: "gatk4_denoisereadcounts" description: Denoises read counts to produce denoised copy ratios keywords: - - gatk4 - - denoisereadcounts - copyratios + - denoisereadcounts + - gatk4 tools: - gatk4: description: | @@ -15,7 +15,6 @@ tools: doi: 10.1158/1538-7445.AM2017-3590 tool_dev_url: "https://github.com/broadinstitute/gatk" licence: ["Apache-2.0"] - input: - meta: type: map @@ -35,7 +34,6 @@ input: type: file description: Panel of normals file hdf5 or tsv format. pattern: "*.{hdf5}" - output: - meta: type: map @@ -54,6 +52,7 @@ output: type: file description: Denoised copy ratios file pattern: "*.{tsv}" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf b/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf index 3c21e743..5af101f8 100644 --- a/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf +++ b/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf @@ -39,7 +39,8 @@ process GATK4_DETERMINEGERMLINECONTIGPLOIDY { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" DetermineGermlineContigPloidy \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + DetermineGermlineContigPloidy \\ ${input_list} \\ --output ./ \\ --output-prefix ${prefix} \\ diff --git a/modules/nf-core/gatk4/determinegermlinecontigploidy/meta.yml b/modules/nf-core/gatk4/determinegermlinecontigploidy/meta.yml index 667d622e..56eb4089 100644 --- a/modules/nf-core/gatk4/determinegermlinecontigploidy/meta.yml +++ b/modules/nf-core/gatk4/determinegermlinecontigploidy/meta.yml @@ -1,10 +1,10 @@ name: "gatk4_determinegermlinecontigploidy" description: Determines the baseline contig ploidy for germline samples given counts data keywords: - - gatk4 - - determinegermlinecontigploidy - - counts - copy number + - counts + - determinegermlinecontigploidy + - gatk4 tools: - gatk4: description: | @@ -15,7 +15,6 @@ tools: documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s doi: 10.1158/1538-7445.AM2017-3590 licence: ["Apache-2.0"] - input: - meta: type: map @@ -49,7 +48,6 @@ input: Optional - A folder containing the ploidy model. When a model is supplied to tool will run in CASE mode. pattern: '*-model/' - output: - meta: type: map @@ -70,6 +68,7 @@ output: A folder containing the model from the input files. This will only be created in COHORT mode (when no model is supplied to the process). pattern: "*-model/" - authors: - "@nvnieuwk" +maintainers: + - "@nvnieuwk" diff --git a/modules/nf-core/gatk4/filtermutectcalls/main.nf b/modules/nf-core/gatk4/filtermutectcalls/main.nf index d0cf5b4a..fa6b46ab 100644 --- a/modules/nf-core/gatk4/filtermutectcalls/main.nf +++ b/modules/nf-core/gatk4/filtermutectcalls/main.nf @@ -2,7 +2,7 @@ process GATK4_FILTERMUTECTCALLS { tag "$meta.id" label 'process_low' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -38,7 +38,8 @@ process GATK4_FILTERMUTECTCALLS { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" FilterMutectCalls \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + FilterMutectCalls \\ --variant $vcf \\ --output ${prefix}.vcf.gz \\ --reference $fasta \\ diff --git a/modules/nf-core/gatk4/filtermutectcalls/meta.yml b/modules/nf-core/gatk4/filtermutectcalls/meta.yml index 1a6faecb..736c8386 100644 --- a/modules/nf-core/gatk4/filtermutectcalls/meta.yml +++ b/modules/nf-core/gatk4/filtermutectcalls/meta.yml @@ -3,9 +3,10 @@ description: | Filters the raw output of mutect2, can optionally use outputs of calculatecontamination and learnreadorientationmodel to improve filtering. keywords: - filtermutectcalls - - mutect2 + - filter - gatk4 - - filtervcf + - mutect2 + - vcf tools: - gatk4: description: | @@ -15,7 +16,6 @@ tools: homepage: https://gatk.broadinstitute.org/hc/en-us documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s doi: 10.1158/1538-7445.AM2017-3590 - input: - meta: type: map @@ -76,7 +76,6 @@ input: type: file description: GATK sequence dictionary pattern: "*.dict" - output: - vcf: type: file @@ -94,8 +93,11 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@GCJMackenzie" - "@maxulysse" - "@ramprasadn" +maintainers: + - "@GCJMackenzie" + - "@maxulysse" + - "@ramprasadn" diff --git a/modules/nf-core/gatk4/germlinecnvcaller/main.nf b/modules/nf-core/gatk4/germlinecnvcaller/main.nf index 441e730f..f42ceb6a 100644 --- a/modules/nf-core/gatk4/germlinecnvcaller/main.nf +++ b/modules/nf-core/gatk4/germlinecnvcaller/main.nf @@ -9,8 +9,9 @@ process GATK4_GERMLINECNVCALLER { tuple val(meta), path(tsv), path(intervals), path(ploidy), path(model) output: - tuple val(meta), path("*-cnv-calls/*-calls"), emit: calls, optional: true - tuple val(meta), path("*-cnv-model/*-model"), emit: model, optional: true + tuple val(meta), path("*-cnv-model/*-calls"), emit: cohortcalls, optional: true + tuple val(meta), path("*-cnv-model/*-model"), emit: cohortmodel, optional: true + tuple val(meta), path("*-cnv-calls/*-calls"), emit: casecalls , optional: true path "versions.yml" , emit: versions when: @@ -36,7 +37,8 @@ process GATK4_GERMLINECNVCALLER { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" GermlineCNVCaller \\ + gatk --java-options "-Xmx${avail_mem}g -XX:-UsePerfData" \\ + GermlineCNVCaller \\ $input_list \\ $ploidy_command \\ $output_command \\ @@ -60,6 +62,7 @@ process GATK4_GERMLINECNVCALLER { """ mkdir -p ${prefix}-cnv-calls/${prefix}-calls mkdir -p ${prefix}-cnv-model/${prefix}-model + mkdir -p ${prefix}-cnv-model/${prefix}-calls cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/gatk4/germlinecnvcaller/meta.yml b/modules/nf-core/gatk4/germlinecnvcaller/meta.yml index b7430927..d4715ff0 100644 --- a/modules/nf-core/gatk4/germlinecnvcaller/meta.yml +++ b/modules/nf-core/gatk4/germlinecnvcaller/meta.yml @@ -2,19 +2,15 @@ name: "gatk4_germlinecnvcaller" description: Calls copy-number variants in germline samples given their counts and the output of DetermineGermlineContigPloidy. keywords: - gatk - - gatk4_germlinecnvcaller - germline contig ploidy + - germlinecnvcaller tools: - "gatk4": - description: - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. + description: Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. homepage: https://gatk.broadinstitute.org/hc/en-us documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s doi: "10.1158/1538-7445.AM2017-3590" licence: ["Apache-2.0"] - input: - meta: type: map @@ -34,10 +30,9 @@ input: description: Optional - directory containing the model produced by germlinecnvcaller cohort mode pattern: "*-cnv-model/*-model" - ploidy: - type: file + type: directory description: Directory containing ploidy calls produced by determinegermlinecontigploidy case or cohort mode pattern: "*-calls" - output: - meta: type: map @@ -48,15 +43,21 @@ output: type: file description: File containing software versions pattern: "versions.yml" - - calls: - type: file + - cohortcalls: + type: directory description: Tar gzipped directory containing calls produced by germlinecnvcaller case mode - pattern: "*-cnv-calls/*-calls" - - model: + pattern: "*-cnv-model/*-calls" + - cohortmodel: type: directory description: Optional - Tar gzipped directory containing the model produced by germlinecnvcaller cohort mode pattern: "*-cnv-model/*-model" - + - casecalls: + type: directory + description: Tar gzipped directory containing calls produced by germlinecnvcaller case mode + pattern: "*-cnv-calls/*-calls" authors: - "@ryanjameskennedy" - "@ViktorHy" +maintainers: + - "@ryanjameskennedy" + - "@ViktorHy" diff --git a/modules/nf-core/gatk4/intervallisttools/main.nf b/modules/nf-core/gatk4/intervallisttools/main.nf index 0054659a..10baa752 100644 --- a/modules/nf-core/gatk4/intervallisttools/main.nf +++ b/modules/nf-core/gatk4/intervallisttools/main.nf @@ -2,7 +2,7 @@ process GATK4_INTERVALLISTTOOLS { tag "$meta.id" label 'process_medium' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -31,7 +31,8 @@ process GATK4_INTERVALLISTTOOLS { mkdir ${prefix}_split - gatk --java-options "-Xmx${avail_mem}M" IntervalListTools \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + IntervalListTools \\ --INPUT $intervals \\ --OUTPUT ${prefix}_split \\ --TMP_DIR . \\ diff --git a/modules/nf-core/gatk4/intervallisttools/meta.yml b/modules/nf-core/gatk4/intervallisttools/meta.yml index 804645f3..748dccfc 100644 --- a/modules/nf-core/gatk4/intervallisttools/meta.yml +++ b/modules/nf-core/gatk4/intervallisttools/meta.yml @@ -1,10 +1,10 @@ name: gatk4_intervallisttools - description: Splits the interval list file into unique, equally-sized interval files and place it under a directory keywords: - - sort - bed - - interval list + - gatk4 + - interval_list + - sort tools: - gatk4: description: | @@ -15,19 +15,16 @@ tools: documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s doi: 10.1158/1538-7445.AM2017-3590 licence: ["Apache-2.0"] - input: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - - interval_list: type: file description: Interval list file pattern: "*.interval_list" - output: - meta: type: map @@ -42,6 +39,7 @@ output: type: file description: Interval list files pattern: "*.interval_list" - authors: - "@praveenraj2018" +maintainers: + - "@praveenraj2018" diff --git a/modules/nf-core/gatk4/mergebamalignment/main.nf b/modules/nf-core/gatk4/mergebamalignment/main.nf index 35d2e71e..f970421f 100644 --- a/modules/nf-core/gatk4/mergebamalignment/main.nf +++ b/modules/nf-core/gatk4/mergebamalignment/main.nf @@ -2,7 +2,7 @@ process GATK4_MERGEBAMALIGNMENT { tag "$meta.id" label 'process_low' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -30,7 +30,8 @@ process GATK4_MERGEBAMALIGNMENT { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" MergeBamAlignment \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + MergeBamAlignment \\ --UNMAPPED_BAM $unmapped \\ --ALIGNED_BAM $aligned \\ --OUTPUT ${prefix}.bam \\ diff --git a/modules/nf-core/gatk4/mergebamalignment/meta.yml b/modules/nf-core/gatk4/mergebamalignment/meta.yml index 9d8ae84b..af4a3f18 100644 --- a/modules/nf-core/gatk4/mergebamalignment/meta.yml +++ b/modules/nf-core/gatk4/mergebamalignment/meta.yml @@ -3,6 +3,7 @@ description: Merge unmapped with mapped BAM files keywords: - alignment - bam + - gatk4 - merge - mergebamalignment tools: @@ -57,3 +58,6 @@ output: authors: - "@kevinmenden" - "@ramprasadn" +maintainers: + - "@kevinmenden" + - "@ramprasadn" diff --git a/modules/nf-core/gatk4/mergevcfs/main.nf b/modules/nf-core/gatk4/mergevcfs/main.nf index dfb5b33a..3362c2bd 100644 --- a/modules/nf-core/gatk4/mergevcfs/main.nf +++ b/modules/nf-core/gatk4/mergevcfs/main.nf @@ -2,7 +2,7 @@ process GATK4_MERGEVCFS { tag "$meta.id" label 'process_medium' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -32,7 +32,8 @@ process GATK4_MERGEVCFS { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" MergeVcfs \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + MergeVcfs \\ $input_list \\ --OUTPUT ${prefix}.vcf.gz \\ $reference_command \\ diff --git a/modules/nf-core/gatk4/mergevcfs/meta.yml b/modules/nf-core/gatk4/mergevcfs/meta.yml index db8c4cb0..30290a85 100644 --- a/modules/nf-core/gatk4/mergevcfs/meta.yml +++ b/modules/nf-core/gatk4/mergevcfs/meta.yml @@ -1,8 +1,9 @@ name: gatk4_mergevcfs description: Merges several vcf files keywords: - - vcf + - gatk4 - merge + - vcf tools: - gatk4: description: | @@ -32,7 +33,6 @@ input: type: file description: Optional Sequence Dictionary as input pattern: "*.dict" - output: - vcf: type: file @@ -42,10 +42,11 @@ output: type: file description: index files for the merged vcf files pattern: "*.tbi" - - versions: type: file description: File containing software versions pattern: "versions.yml" authors: - "@kevinmenden" +maintainers: + - "@kevinmenden" diff --git a/modules/nf-core/gatk4/mutect2/main.nf b/modules/nf-core/gatk4/mutect2/main.nf index bddc3688..721e94f3 100644 --- a/modules/nf-core/gatk4/mutect2/main.nf +++ b/modules/nf-core/gatk4/mutect2/main.nf @@ -2,7 +2,7 @@ process GATK4_MUTECT2 { tag "$meta.id" label 'process_medium' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -42,7 +42,8 @@ process GATK4_MUTECT2 { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" Mutect2 \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + Mutect2 \\ $inputs \\ --output ${prefix}.vcf.gz \\ --reference $fasta \\ diff --git a/modules/nf-core/gatk4/mutect2/meta.yml b/modules/nf-core/gatk4/mutect2/meta.yml index 4842c229..21c928ed 100644 --- a/modules/nf-core/gatk4/mutect2/meta.yml +++ b/modules/nf-core/gatk4/mutect2/meta.yml @@ -2,8 +2,10 @@ name: gatk4_mutect2 description: Call somatic SNVs and indels via local assembly of haplotypes. keywords: - gatk4 - - mutect2 - haplotype + - indels + - mutect2 + - snvs - somatic tools: - gatk4: @@ -15,7 +17,6 @@ tools: documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s doi: 10.1158/1538-7445.AM2017-3590 licence: ["Apache-2.0"] - input: - meta: type: map @@ -77,7 +78,6 @@ input: type: file description: Index for the panel of normals. pattern: "*.vcf.gz.tbi" - output: - vcf: type: file @@ -99,7 +99,9 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@GCJMackenzie" - "@ramprasadn" +maintainers: + - "@GCJMackenzie" + - "@ramprasadn" diff --git a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf index d916c2af..ef9a34a3 100644 --- a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf +++ b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf @@ -35,7 +35,8 @@ process GATK4_POSTPROCESSGERMLINECNVCALLS { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" PostprocessGermlineCNVCalls \\ + gatk --java-options "-Xmx${avail_mem}g -XX:-UsePerfData" \\ + PostprocessGermlineCNVCalls \\ $calls_command \\ $model_command \\ $ploidy_command \\ diff --git a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/meta.yml b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/meta.yml index 92e06cae..a724da11 100644 --- a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/meta.yml +++ b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/meta.yml @@ -1,9 +1,9 @@ name: "gatk4_postprocessgermlinecnvcalls" description: Postprocesses the output of GermlineCNVCaller and generates VCFs and denoised copy ratios keywords: + - copy number - gatk4 - postprocessgermlinecnvcalls - - copy number tools: - gatk4: description: | @@ -14,7 +14,6 @@ tools: documentation: https://gatk.broadinstitute.org/hc/en-us/articles/360037593411-PostprocessGermlineCNVCalls doi: 10.1158/1538-7445.AM2017-3590 licence: ["Apache-2.0"] - input: - meta: type: map @@ -37,7 +36,6 @@ input: A folder containing the model from the input files. This will only be created in COHORT mode (when no model is supplied to the process). pattern: "*-cnv-model/*-model" - output: - meta: type: map @@ -60,6 +58,7 @@ output: type: file description: Intervals VCF file pattern: "*.vcf.gz" - authors: - "@ryanjameskennedy" +maintainers: + - "@ryanjameskennedy" diff --git a/modules/nf-core/gatk4/preprocessintervals/main.nf b/modules/nf-core/gatk4/preprocessintervals/main.nf index aff482f7..61b281da 100644 --- a/modules/nf-core/gatk4/preprocessintervals/main.nf +++ b/modules/nf-core/gatk4/preprocessintervals/main.nf @@ -2,7 +2,7 @@ process GATK4_PREPROCESSINTERVALS { tag "$fasta" label 'process_medium' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -35,7 +35,8 @@ process GATK4_PREPROCESSINTERVALS { } """ - gatk --java-options "-Xmx${avail_mem}M" PreprocessIntervals \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + PreprocessIntervals \\ $include_command \\ $exclude_command \\ --reference $fasta \\ diff --git a/modules/nf-core/gatk4/preprocessintervals/meta.yml b/modules/nf-core/gatk4/preprocessintervals/meta.yml index 8b6ae9b3..cf3f6ac4 100644 --- a/modules/nf-core/gatk4/preprocessintervals/meta.yml +++ b/modules/nf-core/gatk4/preprocessintervals/meta.yml @@ -1,21 +1,17 @@ name: "gatk4_preprocessintervals" description: Prepares bins for coverage collection. keywords: + - bed - gatk4 - - preprocessintervals - interval - - bed + - preprocessintervals tools: - "gatk4": - description: - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. + description: Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. homepage: https://gatk.broadinstitute.org/hc/en-us documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s doi: "10.1158/1538-7445.AM2017-3590" licence: ["Apache-2.0"] - input: - meta: type: map @@ -62,7 +58,6 @@ input: type: file description: Interval file (bed or interval_list) with the genomic regions to be excluded from the analysis (optional) pattern: "*.{bed,interval_list}" - output: - meta: type: map @@ -77,8 +72,11 @@ output: type: file description: Processed interval list file pattern: "*.{bed,interval_list}" - authors: - "@ryanjameskennedy" - "@ViktorHy" - "@ramprasadn" +maintainers: + - "@ryanjameskennedy" + - "@ViktorHy" + - "@ramprasadn" diff --git a/modules/nf-core/gatk4/printreads/main.nf b/modules/nf-core/gatk4/printreads/main.nf index 084d0b46..281096bb 100644 --- a/modules/nf-core/gatk4/printreads/main.nf +++ b/modules/nf-core/gatk4/printreads/main.nf @@ -2,7 +2,7 @@ process GATK4_PRINTREADS { tag "$meta.id" label 'process_single' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -36,7 +36,8 @@ process GATK4_PRINTREADS { error("Output filename is the same as input filename. Please specify a different prefix.") } """ - gatk --java-options "-Xmx${avail_mem}M" PrintReads \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + PrintReads \\ $args \\ --reference $fasta \\ --input $input \\ diff --git a/modules/nf-core/gatk4/printreads/meta.yml b/modules/nf-core/gatk4/printreads/meta.yml index 8150c7a7..aca7e188 100644 --- a/modules/nf-core/gatk4/printreads/meta.yml +++ b/modules/nf-core/gatk4/printreads/meta.yml @@ -1,11 +1,11 @@ name: "gatk4_printreads" description: Print reads in the SAM/BAM/CRAM file keywords: - - gatk4 - bam - cram - - sam + - gatk4 - printreads + - sam tools: - gatk4: description: | @@ -16,7 +16,6 @@ tools: documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s doi: 10.1158/1538-7445.AM2017-3590 licence: ["Apache-2.0"] - input: - meta: type: map @@ -58,7 +57,6 @@ input: type: file description: reference fasta dictionary file pattern: "*.{dict}" - output: - meta: type: map @@ -81,6 +79,7 @@ output: type: file description: Sorted SAM file pattern: "*.{sam}" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/gatk4/revertsam/main.nf b/modules/nf-core/gatk4/revertsam/main.nf index 768b1eed..da3a71f9 100644 --- a/modules/nf-core/gatk4/revertsam/main.nf +++ b/modules/nf-core/gatk4/revertsam/main.nf @@ -2,7 +2,7 @@ process GATK4_REVERTSAM { tag "$meta.id" label 'process_medium' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -28,7 +28,8 @@ process GATK4_REVERTSAM { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" RevertSam \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + RevertSam \\ --INPUT $bam \\ --OUTPUT ${prefix}.reverted.bam \\ --TMP_DIR . \\ diff --git a/modules/nf-core/gatk4/revertsam/meta.yml b/modules/nf-core/gatk4/revertsam/meta.yml index 6cc97d86..ac6c0d0f 100644 --- a/modules/nf-core/gatk4/revertsam/meta.yml +++ b/modules/nf-core/gatk4/revertsam/meta.yml @@ -1,8 +1,9 @@ name: gatk4_revertsam description: Reverts SAM or BAM files to a previous state. keywords: - - sam + - gatk4 - revert + - sam tools: - gatk4: description: | @@ -34,3 +35,5 @@ output: pattern: "versions.yml" authors: - "@kevinmenden" +maintainers: + - "@kevinmenden" diff --git a/modules/nf-core/gatk4/samtofastq/main.nf b/modules/nf-core/gatk4/samtofastq/main.nf index f838b95a..02bf2152 100644 --- a/modules/nf-core/gatk4/samtofastq/main.nf +++ b/modules/nf-core/gatk4/samtofastq/main.nf @@ -2,7 +2,7 @@ process GATK4_SAMTOFASTQ { tag "$meta.id" label 'process_medium' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -29,7 +29,8 @@ process GATK4_SAMTOFASTQ { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" SamToFastq \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + SamToFastq \\ --INPUT $bam \\ $output \\ --TMP_DIR . \\ diff --git a/modules/nf-core/gatk4/samtofastq/meta.yml b/modules/nf-core/gatk4/samtofastq/meta.yml index 60ca6aee..b61ef4fc 100644 --- a/modules/nf-core/gatk4/samtofastq/meta.yml +++ b/modules/nf-core/gatk4/samtofastq/meta.yml @@ -2,7 +2,8 @@ name: gatk4_samtofastq description: Converts BAM/SAM file to FastQ format keywords: - bed - - interval list + - gatk4 + - interval_list tools: - gatk4: description: | @@ -34,3 +35,5 @@ output: pattern: "versions.yml" authors: - "@kevinmenden" +maintainers: + - "@kevinmenden" diff --git a/modules/nf-core/gatk4/selectvariants/main.nf b/modules/nf-core/gatk4/selectvariants/main.nf index 609cb8cc..f81a019b 100644 --- a/modules/nf-core/gatk4/selectvariants/main.nf +++ b/modules/nf-core/gatk4/selectvariants/main.nf @@ -2,7 +2,7 @@ process GATK4_SELECTVARIANTS { tag "$meta.id" label 'process_medium' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -30,7 +30,8 @@ process GATK4_SELECTVARIANTS { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" SelectVariants \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + SelectVariants \\ --variant $vcf \\ --output ${prefix}.selectvariants.vcf.gz \\ $interval \\ diff --git a/modules/nf-core/gatk4/selectvariants/meta.yml b/modules/nf-core/gatk4/selectvariants/meta.yml index 46605d15..5bd0fc69 100644 --- a/modules/nf-core/gatk4/selectvariants/meta.yml +++ b/modules/nf-core/gatk4/selectvariants/meta.yml @@ -1,7 +1,6 @@ name: gatk4_selectvariants description: Select a subset of variants from a VCF file keywords: - - gatk - gatk4 - selectvariants - vcf @@ -16,7 +15,6 @@ tools: tool_dev_url: https://github.com/broadinstitute/gatk doi: 10.1158/1538-7445.AM2017-3590 licence: ["Apache-2.0"] - input: - meta: type: map @@ -35,7 +33,6 @@ input: type: file description: One or more genomic intervals over which to operate pattern: ".intervals" - output: - meta: type: map @@ -54,7 +51,9 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@mjcipriano" - "@ramprasadn" +maintainers: + - "@mjcipriano" + - "@ramprasadn" diff --git a/modules/nf-core/gatk4/shiftfasta/main.nf b/modules/nf-core/gatk4/shiftfasta/main.nf index ab0e578c..b0ff3ed0 100644 --- a/modules/nf-core/gatk4/shiftfasta/main.nf +++ b/modules/nf-core/gatk4/shiftfasta/main.nf @@ -2,7 +2,7 @@ process GATK4_SHIFTFASTA { tag "$meta.id" label 'process_single' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -36,7 +36,8 @@ process GATK4_SHIFTFASTA { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" ShiftFasta \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + ShiftFasta \\ --reference $fasta \\ --output ${prefix}_shift.fasta \\ --shift-back-output ${prefix}_shift.back_chain \\ diff --git a/modules/nf-core/gatk4/shiftfasta/meta.yml b/modules/nf-core/gatk4/shiftfasta/meta.yml index 6d563ded..c1ab0c2f 100644 --- a/modules/nf-core/gatk4/shiftfasta/meta.yml +++ b/modules/nf-core/gatk4/shiftfasta/meta.yml @@ -1,9 +1,10 @@ name: "gatk4_shiftfasta" description: Create a fasta with the bases shifted by offset keywords: + - gatk4 - mitochondria - - shiftfasta - shiftchain + - shiftfasta - shiftintervals tools: - gatk4: @@ -16,7 +17,6 @@ tools: tool_dev_url: "https://github.com/broadinstitute/gatk" doi: 10.1158/1538-7445.AM2017-3590 licence: ["Apache-2.0"] - input: - meta: type: map @@ -45,7 +45,6 @@ input: type: file description: sequence dictionary file pattern: "*.{dict}" - output: - meta: type: map @@ -76,6 +75,7 @@ output: type: file description: Intervals file for the shifted fasta file pattern: "*.{shifted.intervals}" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/gatk4/splitintervals/main.nf b/modules/nf-core/gatk4/splitintervals/main.nf index 3cb18373..957f1675 100644 --- a/modules/nf-core/gatk4/splitintervals/main.nf +++ b/modules/nf-core/gatk4/splitintervals/main.nf @@ -2,7 +2,7 @@ process GATK4_SPLITINTERVALS { tag "$meta.id" label 'process_low' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -32,7 +32,8 @@ process GATK4_SPLITINTERVALS { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" SplitIntervals \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + SplitIntervals \\ --output ${prefix} \\ --intervals $intervals \\ $reference \\ diff --git a/modules/nf-core/gatk4/splitintervals/meta.yml b/modules/nf-core/gatk4/splitintervals/meta.yml index a249f077..c92bad8b 100644 --- a/modules/nf-core/gatk4/splitintervals/meta.yml +++ b/modules/nf-core/gatk4/splitintervals/meta.yml @@ -1,8 +1,9 @@ name: gatk4_splitintervals description: Split intervals into sub-interval files. keywords: - - interval - bed + - gatk4 + - interval - splitintervals tools: - gatk4: @@ -12,7 +13,6 @@ tools: tool_dev_url: https://github.com/broadinstitute/gatk doi: "10.1158/1538-7445.AM2017-3590" licence: ["BSD-3-clause"] - input: - meta: type: map @@ -50,7 +50,6 @@ input: type: file description: Reference sequence dictionary pattern: "*.dict" - output: - meta: type: map @@ -65,7 +64,9 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@nvnieuwk" - "@ramprasadn" +maintainers: + - "@nvnieuwk" + - "@ramprasadn" diff --git a/modules/nf-core/gatk4/variantfiltration/main.nf b/modules/nf-core/gatk4/variantfiltration/main.nf index 387ff8ca..1c4d8ad0 100644 --- a/modules/nf-core/gatk4/variantfiltration/main.nf +++ b/modules/nf-core/gatk4/variantfiltration/main.nf @@ -2,7 +2,7 @@ process GATK4_VARIANTFILTRATION { tag "$meta.id" label 'process_medium' - conda "bioconda::gatk4=4.4.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" @@ -32,7 +32,8 @@ process GATK4_VARIANTFILTRATION { avail_mem = (task.memory.mega*0.8).intValue() } """ - gatk --java-options "-Xmx${avail_mem}M" VariantFiltration \\ + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ + VariantFiltration \\ --variant $vcf \\ --output ${prefix}.vcf.gz \\ --reference $fasta \\ diff --git a/modules/nf-core/gatk4/variantfiltration/meta.yml b/modules/nf-core/gatk4/variantfiltration/meta.yml index 2260f37b..11915a94 100644 --- a/modules/nf-core/gatk4/variantfiltration/meta.yml +++ b/modules/nf-core/gatk4/variantfiltration/meta.yml @@ -1,9 +1,10 @@ name: gatk4_variantfiltration description: Filter variants keywords: - - vcf - filter + - gatk4 - variantfiltration + - vcf tools: - gatk4: description: | @@ -71,3 +72,6 @@ output: authors: - "@kevinmenden" - "@ramprasadn" +maintainers: + - "@kevinmenden" + - "@ramprasadn" diff --git a/modules/nf-core/genmod/annotate/main.nf b/modules/nf-core/genmod/annotate/main.nf index 43ae0d19..13b7aff8 100644 --- a/modules/nf-core/genmod/annotate/main.nf +++ b/modules/nf-core/genmod/annotate/main.nf @@ -2,7 +2,7 @@ process GENMOD_ANNOTATE { tag "$meta.id" label 'process_medium' - conda "bioconda::genmod=3.7.4" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/genmod:3.7.4--pyh5e36f6f_0': 'biocontainers/genmod:3.7.4--pyh5e36f6f_0' }" diff --git a/modules/nf-core/genmod/annotate/meta.yml b/modules/nf-core/genmod/annotate/meta.yml index b142f96b..81aac686 100644 --- a/modules/nf-core/genmod/annotate/meta.yml +++ b/modules/nf-core/genmod/annotate/meta.yml @@ -9,7 +9,6 @@ tools: homepage: "https://github.com/Clinical-Genomics/genmod" documentation: "https://github.com/Clinical-Genomics/genmod" tool_dev_url: "https://github.com/moonso" - licence: "['MIT']" input: - meta: @@ -35,6 +34,7 @@ output: type: vcf description: Annotated VCF file pattern: "*.{vcf}" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/genmod/compound/main.nf b/modules/nf-core/genmod/compound/main.nf index 149a03d8..d58f5fc4 100644 --- a/modules/nf-core/genmod/compound/main.nf +++ b/modules/nf-core/genmod/compound/main.nf @@ -2,7 +2,7 @@ process GENMOD_COMPOUND { tag "$meta.id" label 'process_medium' - conda "bioconda::genmod=3.7.4" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/genmod:3.7.4--pyh5e36f6f_0': 'biocontainers/genmod:3.7.4--pyh5e36f6f_0' }" diff --git a/modules/nf-core/genmod/compound/meta.yml b/modules/nf-core/genmod/compound/meta.yml index 831ba341..378bedfc 100644 --- a/modules/nf-core/genmod/compound/meta.yml +++ b/modules/nf-core/genmod/compound/meta.yml @@ -9,7 +9,6 @@ tools: homepage: "https://github.com/Clinical-Genomics/genmod" documentation: "https://github.com/Clinical-Genomics/genmod" tool_dev_url: "https://github.com/moonso" - licence: "['MIT']" input: - meta: @@ -35,6 +34,7 @@ output: type: file description: Output VCF file pattern: "*.{vcf}" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/genmod/models/main.nf b/modules/nf-core/genmod/models/main.nf index 3f5700d6..15dc077b 100644 --- a/modules/nf-core/genmod/models/main.nf +++ b/modules/nf-core/genmod/models/main.nf @@ -2,7 +2,7 @@ process GENMOD_MODELS { tag "$meta.id" label 'process_medium' - conda "bioconda::genmod=3.7.4 conda-forge::python=3.4.5" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/genmod:3.7.4--pyh5e36f6f_0': 'biocontainers/genmod:3.7.4--pyh5e36f6f_0' }" diff --git a/modules/nf-core/genmod/models/meta.yml b/modules/nf-core/genmod/models/meta.yml index 240f79df..ea3295ac 100644 --- a/modules/nf-core/genmod/models/meta.yml +++ b/modules/nf-core/genmod/models/meta.yml @@ -9,7 +9,6 @@ tools: homepage: "https://github.com/Clinical-Genomics/genmod" documentation: "https://github.com/Clinical-Genomics/genmod" tool_dev_url: "https://github.com/moonso" - licence: "['MIT']" input: - meta: @@ -29,7 +28,6 @@ input: type: file description: ped file pattern: "*.{ped}" - output: - meta: type: map @@ -44,6 +42,7 @@ output: type: file description: Output VCF file pattern: "*.{vcf}" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/genmod/score/main.nf b/modules/nf-core/genmod/score/main.nf index d1efade5..362ca074 100644 --- a/modules/nf-core/genmod/score/main.nf +++ b/modules/nf-core/genmod/score/main.nf @@ -2,7 +2,7 @@ process GENMOD_SCORE { tag "$meta.id" label 'process_medium' - conda "bioconda::genmod=3.7.4" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/genmod:3.7.4--pyh5e36f6f_0': 'biocontainers/genmod:3.7.4--pyh5e36f6f_0' }" diff --git a/modules/nf-core/genmod/score/meta.yml b/modules/nf-core/genmod/score/meta.yml index 26bb22ae..49dddcb1 100644 --- a/modules/nf-core/genmod/score/meta.yml +++ b/modules/nf-core/genmod/score/meta.yml @@ -9,7 +9,6 @@ tools: homepage: "https://github.com/Clinical-Genomics/genmod" documentation: "https://github.com/Clinical-Genomics/genmod" tool_dev_url: "https://github.com/moonso" - licence: "['MIT']" input: - meta: @@ -29,7 +28,6 @@ input: type: file description: rank model config file pattern: "*.{ini}" - output: - meta: type: map @@ -44,6 +42,7 @@ output: type: file description: Output VCF file pattern: "*.{vcf}" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/glnexus/main.nf b/modules/nf-core/glnexus/main.nf index 2bd4580a..eb86b9b8 100644 --- a/modules/nf-core/glnexus/main.nf +++ b/modules/nf-core/glnexus/main.nf @@ -2,7 +2,7 @@ process GLNEXUS { tag "$meta.id" label 'process_medium' - conda "bioconda::glnexus=1.4.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/glnexus:1.4.1--h40d77a6_0' : 'biocontainers/glnexus:1.4.1--h40d77a6_0' }" diff --git a/modules/nf-core/glnexus/meta.yml b/modules/nf-core/glnexus/meta.yml index 89e4c74e..4944ebde 100644 --- a/modules/nf-core/glnexus/meta.yml +++ b/modules/nf-core/glnexus/meta.yml @@ -8,10 +8,8 @@ tools: description: scalable gVCF merging and joint variant calling for population sequencing projects. homepage: https://github.com/dnanexus-rnd/GLnexus documentation: https://github.com/dnanexus-rnd/GLnexus/wiki/Getting-Started - doi: 10.1101/343970 licence: ["Apache-2.0"] - input: - meta: type: map @@ -22,7 +20,6 @@ input: type: list description: Input genomic vcf files pattern: "*.{gvcf,gvcf.gz,g.vcf,g.vcf.gz}" - output: - versions: type: file @@ -34,3 +31,5 @@ output: pattern: "*.bcf" authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/haplocheck/main.nf b/modules/nf-core/haplocheck/main.nf index 464f5dbf..0d26c449 100644 --- a/modules/nf-core/haplocheck/main.nf +++ b/modules/nf-core/haplocheck/main.nf @@ -2,7 +2,7 @@ process HAPLOCHECK { tag "$meta.id" label 'process_low' - conda "bioconda::haplocheck=1.3.3" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/haplocheck:1.3.3--h4a94de4_0': 'biocontainers/haplocheck:1.3.3--h4a94de4_0' }" diff --git a/modules/nf-core/haplocheck/meta.yml b/modules/nf-core/haplocheck/meta.yml index 79da6a40..273b5b1c 100644 --- a/modules/nf-core/haplocheck/meta.yml +++ b/modules/nf-core/haplocheck/meta.yml @@ -16,40 +16,35 @@ tools: tool_dev_url: "https://github.com/genepi/haplocheck" doi: 10.1101/gr.256545.119 licence: "['MIT']" - input: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - - vcf: type: file description: VCF file pattern: "*.{vcf.gz}" - output: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - - versions: type: file description: File containing software versions pattern: "versions.yml" - - txt: type: file description: Raw report in txt format pattern: "*.{txt}" - - html: type: file description: Haplocheck HTML report pattern: "*.{html}" - authors: - "@lmtani" +maintainers: + - "@lmtani" diff --git a/modules/nf-core/haplogrep2/classify/main.nf b/modules/nf-core/haplogrep2/classify/main.nf index 7f775cae..8ef9a033 100644 --- a/modules/nf-core/haplogrep2/classify/main.nf +++ b/modules/nf-core/haplogrep2/classify/main.nf @@ -2,7 +2,7 @@ process HAPLOGREP2_CLASSIFY { tag "$meta.id" label 'process_low' - conda "bioconda::haplogrep=2.4.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/haplogrep:2.4.0--hdfd78af_0': 'biocontainers/haplogrep:2.4.0--hdfd78af_0' }" diff --git a/modules/nf-core/haplogrep2/classify/meta.yml b/modules/nf-core/haplogrep2/classify/meta.yml index d21cc9b5..8174218e 100644 --- a/modules/nf-core/haplogrep2/classify/meta.yml +++ b/modules/nf-core/haplogrep2/classify/meta.yml @@ -8,9 +8,7 @@ tools: homepage: "https://github.com/seppinho/haplogrep-cmd" documentation: "https://github.com/seppinho/haplogrep-cmd" tool_dev_url: "https://github.com/seppinho/haplogrep-cmd" - licence: "['MIT']" - input: - meta: type: map @@ -24,7 +22,6 @@ input: - format: type: string description: either "vcf", "fasta" or "hsd" - output: - meta: type: map @@ -39,6 +36,7 @@ output: type: file description: text file with classification information pattern: "*.{txt}" - authors: - "@lucpen" +maintainers: + - "@lucpen" diff --git a/modules/nf-core/hmtnote/annotate/main.nf b/modules/nf-core/hmtnote/annotate/main.nf index d523d047..2fca73f4 100644 --- a/modules/nf-core/hmtnote/annotate/main.nf +++ b/modules/nf-core/hmtnote/annotate/main.nf @@ -2,7 +2,7 @@ process HMTNOTE_ANNOTATE { tag "$meta.id" label 'process_low' - conda "bioconda::hmtnote=0.7.2" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/hmtnote:0.7.2--pyhdfd78af_1': 'biocontainers/hmtnote:0.7.2--pyhdfd78af_1' }" diff --git a/modules/nf-core/hmtnote/annotate/meta.yml b/modules/nf-core/hmtnote/annotate/meta.yml index 44cf88ca..4ab41831 100644 --- a/modules/nf-core/hmtnote/annotate/meta.yml +++ b/modules/nf-core/hmtnote/annotate/meta.yml @@ -9,10 +9,8 @@ tools: description: Human mitochondrial variants annotation using HmtVar. homepage: https://github.com/robertopreste/HmtNote documentation: https://hmtnote.readthedocs.io/en/latest/usage.html - doi: "10.1101/600619" licence: ["MIT"] - input: - meta: type: map @@ -22,7 +20,6 @@ input: type: file description: vcf file pattern: "*.vcf" - output: - meta: type: map @@ -36,6 +33,7 @@ output: type: file description: annotated vcf pattern: "*_annotated.vcf" - authors: - "@sysbiocoder" +maintainers: + - "@sysbiocoder" diff --git a/modules/nf-core/manta/germline/main.nf b/modules/nf-core/manta/germline/main.nf index e052b7c9..5d5666c6 100644 --- a/modules/nf-core/manta/germline/main.nf +++ b/modules/nf-core/manta/germline/main.nf @@ -3,7 +3,7 @@ process MANTA_GERMLINE { label 'process_medium' label 'error_retry' - conda "bioconda::manta=1.6.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/manta:1.6.0--h9ee0642_1' : 'biocontainers/manta:1.6.0--h9ee0642_1' }" @@ -13,6 +13,7 @@ process MANTA_GERMLINE { tuple val(meta), path(input), path(index), path(target_bed), path(target_bed_tbi) tuple val(meta2), path(fasta) tuple val(meta3), path(fai) + path(config) output: tuple val(meta), path("*candidate_small_indels.vcf.gz") , emit: candidate_small_indels_vcf @@ -31,27 +32,29 @@ process MANTA_GERMLINE { def prefix = task.ext.prefix ?: "${meta.id}" def input_files = input.collect{"--bam ${it}"}.join(' ') def options_manta = target_bed ? "--callRegions $target_bed" : "" + def config_option = config ? "--config ${config}" : "" """ - configManta.py \ - ${input_files} \ - --reference $fasta \ - --runDir manta \ - $options_manta \ + configManta.py \\ + ${input_files} \\ + ${config_option} \\ + --reference $fasta \\ + --runDir manta \\ + $options_manta \\ $args python manta/runWorkflow.py -m local -j $task.cpus - mv manta/results/variants/candidateSmallIndels.vcf.gz \ + mv manta/results/variants/candidateSmallIndels.vcf.gz \\ ${prefix}.candidate_small_indels.vcf.gz - mv manta/results/variants/candidateSmallIndels.vcf.gz.tbi \ + mv manta/results/variants/candidateSmallIndels.vcf.gz.tbi \\ ${prefix}.candidate_small_indels.vcf.gz.tbi - mv manta/results/variants/candidateSV.vcf.gz \ + mv manta/results/variants/candidateSV.vcf.gz \\ ${prefix}.candidate_sv.vcf.gz - mv manta/results/variants/candidateSV.vcf.gz.tbi \ + mv manta/results/variants/candidateSV.vcf.gz.tbi \\ ${prefix}.candidate_sv.vcf.gz.tbi - mv manta/results/variants/diploidSV.vcf.gz \ + mv manta/results/variants/diploidSV.vcf.gz \\ ${prefix}.diploid_sv.vcf.gz - mv manta/results/variants/diploidSV.vcf.gz.tbi \ + mv manta/results/variants/diploidSV.vcf.gz.tbi \\ ${prefix}.diploid_sv.vcf.gz.tbi cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/manta/germline/meta.yml b/modules/nf-core/manta/germline/meta.yml index 2eb16ada..72ed15f8 100644 --- a/modules/nf-core/manta/germline/meta.yml +++ b/modules/nf-core/manta/germline/meta.yml @@ -16,7 +16,6 @@ tools: tool_dev_url: https://github.com/Illumina/manta doi: "10.1093/bioinformatics/btv710" licence: ["GPL v3"] - input: - meta: type: map @@ -57,7 +56,10 @@ input: type: file description: Genome reference FASTA index file pattern: "*.{fa.fai,fasta.fai}" - + - config: + type: file + description: Manta configuration file + pattern: "*.{ini,conf,config}" output: - meta: type: map @@ -92,7 +94,11 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@maxulysse" - "@ramprasadn" + - "@nvnieuwk" +maintainers: + - "@maxulysse" + - "@ramprasadn" + - "@nvnieuwk" diff --git a/modules/nf-core/mosdepth/main.nf b/modules/nf-core/mosdepth/main.nf index 74db3a27..c7e24303 100644 --- a/modules/nf-core/mosdepth/main.nf +++ b/modules/nf-core/mosdepth/main.nf @@ -2,10 +2,10 @@ process MOSDEPTH { tag "$meta.id" label 'process_medium' - conda "bioconda::mosdepth=0.3.3" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mosdepth:0.3.3--hdfd78af_1' : - 'biocontainers/mosdepth:0.3.3--hdfd78af_1'}" + 'https://depot.galaxyproject.org/singularity/mosdepth:0.3.6--hd299d5a_0' : + 'biocontainers/mosdepth:0.3.6--hd299d5a_0'}" input: tuple val(meta), path(bam), path(bai), path(bed) diff --git a/modules/nf-core/mosdepth/meta.yml b/modules/nf-core/mosdepth/meta.yml index adf3893f..9caaf2cd 100644 --- a/modules/nf-core/mosdepth/meta.yml +++ b/modules/nf-core/mosdepth/meta.yml @@ -26,19 +26,14 @@ input: type: file description: Index for BAM/CRAM file pattern: "*.{bai,crai}" - - meta2: - type: map - description: | - Groovy Map containing bed information - e.g. [ id:'test' ] - bed: type: file description: BED file with intersected intervals pattern: "*.{bed}" - - meta3: + - meta2: type: map description: | - Groovy Map containing reference information + Groovy Map containing bed information e.g. [ id:'test' ] - fasta: type: file @@ -107,3 +102,8 @@ authors: - "@drpatelh" - "@ramprasadn" - "@matthdsm" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@ramprasadn" + - "@matthdsm" diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index bc0bdb5b..7625b752 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.18 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 00cc48d2..1b9f7c43 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : - 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -43,7 +43,7 @@ process MULTIQC { stub: """ - touch multiqc_data + mkdir multiqc_data touch multiqc_plots touch multiqc_report.html diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f1aa660e..45a9bc35 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,4 +1,3 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index c2dad217..d0438eda 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -7,12 +7,9 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" - test("MULTIQC: FASTQC") { + test("sarscov2 single-end [fastqc]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -26,20 +23,17 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } ) } } - test("MULTIQC: FASTQC and a config file") { + test("sarscov2 single-end [fastqc] [config]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -53,9 +47,35 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastqc] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.report.collect { file(it).getName() } + + process.out.data.collect { file(it).getName() } + + process.out.plots.collect { file(it).getName() } + + process.out.versions ).match() } ) } diff --git a/modules/nf-core/peddy/main.nf b/modules/nf-core/peddy/main.nf index 6671de73..b6be28c6 100644 --- a/modules/nf-core/peddy/main.nf +++ b/modules/nf-core/peddy/main.nf @@ -2,7 +2,7 @@ process PEDDY { tag "$meta.id" label 'process_low' - conda "bioconda::peddy=0.4.8" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/peddy:0.4.8--pyh5e36f6f_0' : 'biocontainers/peddy:0.4.8--pyh5e36f6f_0' }" diff --git a/modules/nf-core/peddy/meta.yml b/modules/nf-core/peddy/meta.yml index 4c72b286..24a82e19 100644 --- a/modules/nf-core/peddy/meta.yml +++ b/modules/nf-core/peddy/meta.yml @@ -4,7 +4,6 @@ keywords: - pedigrees - ped - family - tools: - peddy: description: genotype, ped correspondence check, ancestry check, sex check. directly, quickly on VCF @@ -13,7 +12,6 @@ tools: tool_dev_url: https://github.com/brentp/peddy doi: "10.1016/j.ajhg.2017.01.017" licence: ["MIT"] - input: - meta: type: map @@ -32,7 +30,6 @@ input: type: file description: TBI file pattern: "*.{vcf.gz.tbi}" - output: - meta: type: map @@ -59,6 +56,7 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@rannick" +maintainers: + - "@rannick" diff --git a/modules/nf-core/picard/addorreplacereadgroups/main.nf b/modules/nf-core/picard/addorreplacereadgroups/main.nf index a2a8d1c3..dc1a387d 100644 --- a/modules/nf-core/picard/addorreplacereadgroups/main.nf +++ b/modules/nf-core/picard/addorreplacereadgroups/main.nf @@ -2,10 +2,10 @@ process PICARD_ADDORREPLACEREADGROUPS { tag "$meta.id" label 'process_low' - conda "bioconda::picard=3.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : - 'biocontainers/picard:3.0.0--hdfd78af_1' }" + 'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' : + 'biocontainers/picard:3.1.1--hdfd78af_0' }" input: tuple val(meta), path(bam) @@ -27,6 +27,9 @@ process PICARD_ADDORREPLACEREADGROUPS { } else { avail_mem = (task.memory.mega*0.8).intValue() } + + if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ picard \\ -Xmx${avail_mem}M \\ @@ -43,6 +46,8 @@ process PICARD_ADDORREPLACEREADGROUPS { stub: def prefix = task.ext.prefix ?: "${meta.id}" + + if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" """ touch ${prefix}.bam diff --git a/modules/nf-core/picard/addorreplacereadgroups/meta.yml b/modules/nf-core/picard/addorreplacereadgroups/meta.yml index 28f584c3..ab573ac8 100644 --- a/modules/nf-core/picard/addorreplacereadgroups/meta.yml +++ b/modules/nf-core/picard/addorreplacereadgroups/meta.yml @@ -4,6 +4,7 @@ keywords: - add - replace - read-group + - picard tools: - picard: description: | @@ -13,7 +14,6 @@ tools: documentation: https://gatk.broadinstitute.org/hc/en-us/articles/360037226472-AddOrReplaceReadGroups-Picard- tool_dev_url: https://github.com/broadinstitute/picard licence: ["MIT"] - input: - meta: type: map @@ -24,7 +24,6 @@ input: type: file description: Input BAM file pattern: "*.{bam}" - output: - meta: type: map @@ -43,10 +42,15 @@ output: type: file description: BAM index file pattern: "*.{bai}" - authors: - "@sateeshperi" - "@mjcipriano" - "@hseabolt" - "@cmatKhan" - "@muffato" +maintainers: + - "@sateeshperi" + - "@mjcipriano" + - "@hseabolt" + - "@cmatKhan" + - "@muffato" diff --git a/modules/nf-core/picard/collecthsmetrics/main.nf b/modules/nf-core/picard/collecthsmetrics/main.nf index d721bc6f..b798452d 100644 --- a/modules/nf-core/picard/collecthsmetrics/main.nf +++ b/modules/nf-core/picard/collecthsmetrics/main.nf @@ -2,10 +2,10 @@ process PICARD_COLLECTHSMETRICS { tag "$meta.id" label 'process_single' - conda "bioconda::picard=3.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : - 'biocontainers/picard:3.0.0--hdfd78af_1' }" + 'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' : + 'biocontainers/picard:3.1.1--hdfd78af_0' }" input: tuple val(meta), path(bam), path(bai), path(bait_intervals), path(target_intervals) diff --git a/modules/nf-core/picard/collecthsmetrics/meta.yml b/modules/nf-core/picard/collecthsmetrics/meta.yml index fecad0e5..4b296fe0 100644 --- a/modules/nf-core/picard/collecthsmetrics/meta.yml +++ b/modules/nf-core/picard/collecthsmetrics/meta.yml @@ -17,7 +17,6 @@ tools: documentation: https://broadinstitute.github.io/picard/ tool_dev_url: https://github.com/broadinstitute/picard/ licence: ["MIT"] - input: - meta: type: map @@ -83,7 +82,9 @@ output: type: file description: Alignment metrics files generated by picard pattern: "*_{metrics}" - authors: - "@projectoriented" - "@matthdsm" +maintainers: + - "@projectoriented" + - "@matthdsm" diff --git a/modules/nf-core/picard/collectmultiplemetrics/main.nf b/modules/nf-core/picard/collectmultiplemetrics/main.nf index 91fe9170..5640ce94 100644 --- a/modules/nf-core/picard/collectmultiplemetrics/main.nf +++ b/modules/nf-core/picard/collectmultiplemetrics/main.nf @@ -2,10 +2,10 @@ process PICARD_COLLECTMULTIPLEMETRICS { tag "$meta.id" label 'process_single' - conda "bioconda::picard=3.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : - 'biocontainers/picard:3.0.0--hdfd78af_1' }" + 'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' : + 'biocontainers/picard:3.1.1--hdfd78af_0' }" input: tuple val(meta) , path(bam), path(bai) @@ -14,7 +14,7 @@ process PICARD_COLLECTMULTIPLEMETRICS { output: tuple val(meta), path("*_metrics"), emit: metrics - tuple val(meta), path("*.pdf") , emit: pdf + tuple val(meta), path("*.pdf") , emit: pdf, optional: true path "versions.yml" , emit: versions when: diff --git a/modules/nf-core/picard/collectmultiplemetrics/meta.yml b/modules/nf-core/picard/collectmultiplemetrics/meta.yml index 22656080..67bba57b 100644 --- a/modules/nf-core/picard/collectmultiplemetrics/meta.yml +++ b/modules/nf-core/picard/collectmultiplemetrics/meta.yml @@ -66,3 +66,5 @@ output: pattern: "versions.yml" authors: - "@drpatelh" +maintainers: + - "@drpatelh" diff --git a/modules/nf-core/picard/collectwgsmetrics/main.nf b/modules/nf-core/picard/collectwgsmetrics/main.nf index 1d59334c..35f4129c 100644 --- a/modules/nf-core/picard/collectwgsmetrics/main.nf +++ b/modules/nf-core/picard/collectwgsmetrics/main.nf @@ -2,10 +2,10 @@ process PICARD_COLLECTWGSMETRICS { tag "$meta.id" label 'process_single' - conda "bioconda::picard=3.0.0 r::r-base" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : - 'biocontainers/picard:3.0.0--hdfd78af_1' }" + 'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' : + 'biocontainers/picard:3.1.1--hdfd78af_0' }" input: tuple val(meta), path(bam), path(bai) diff --git a/modules/nf-core/picard/collectwgsmetrics/meta.yml b/modules/nf-core/picard/collectwgsmetrics/meta.yml index 19906f08..5576ef92 100644 --- a/modules/nf-core/picard/collectwgsmetrics/meta.yml +++ b/modules/nf-core/picard/collectwgsmetrics/meta.yml @@ -68,3 +68,8 @@ authors: - "@flowuenne" - "@lassefolkersen" - "@ramprasadn" +maintainers: + - "@drpatelh" + - "@flowuenne" + - "@lassefolkersen" + - "@ramprasadn" diff --git a/modules/nf-core/picard/liftovervcf/main.nf b/modules/nf-core/picard/liftovervcf/main.nf index bfb004f7..ab7212a1 100644 --- a/modules/nf-core/picard/liftovervcf/main.nf +++ b/modules/nf-core/picard/liftovervcf/main.nf @@ -2,10 +2,10 @@ process PICARD_LIFTOVERVCF { tag "$meta.id" label 'process_low' - conda "bioconda::picard=3.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : - 'biocontainers/picard:3.0.0--hdfd78af_1' }" + 'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' : + 'biocontainers/picard:3.1.1--hdfd78af_0' }" input: tuple val(meta), path(input_vcf) diff --git a/modules/nf-core/picard/liftovervcf/meta.yml b/modules/nf-core/picard/liftovervcf/meta.yml index 6023e5f9..9ccba6d2 100644 --- a/modules/nf-core/picard/liftovervcf/meta.yml +++ b/modules/nf-core/picard/liftovervcf/meta.yml @@ -10,14 +10,11 @@ tools: homepage: https://gatk.broadinstitute.org/hc/en-us/articles/360037060932-LiftoverVcf-Picard documentation: https://gatk.broadinstitute.org/hc/en-us/articles/360037060932-LiftoverVcf-Picard tool_dev_url: https://github.com/broadinstitute/picard - licence: ["MIT"] - input: - meta: type: map - description: Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] + description: Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - input_vcf: type: file description: VCF file @@ -48,12 +45,10 @@ input: - chain: type: file description: The liftover chain file - output: - meta: type: map - description: Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] + description: Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - versions: type: file description: File containing software versions @@ -66,7 +61,9 @@ output: type: file description: VCF file containing unsuccessfully lifted variants pattern: "*.{unlifted.vcf.gz}" - authors: - "@lucpen" - "@ramprasadn" +maintainers: + - "@lucpen" + - "@ramprasadn" diff --git a/modules/nf-core/picard/markduplicates/main.nf b/modules/nf-core/picard/markduplicates/main.nf index facd7efb..80930cc4 100644 --- a/modules/nf-core/picard/markduplicates/main.nf +++ b/modules/nf-core/picard/markduplicates/main.nf @@ -2,10 +2,10 @@ process PICARD_MARKDUPLICATES { tag "$meta.id" label 'process_medium' - conda "bioconda::picard=3.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : - 'biocontainers/picard:3.0.0--hdfd78af_1' }" + 'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' : + 'biocontainers/picard:3.1.1--hdfd78af_0' }" input: tuple val(meta), path(bam) @@ -30,6 +30,9 @@ process PICARD_MARKDUPLICATES { } else { avail_mem = (task.memory.mega*0.8).intValue() } + + if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ picard \\ -Xmx${avail_mem}M \\ @@ -48,6 +51,7 @@ process PICARD_MARKDUPLICATES { stub: def prefix = task.ext.prefix ?: "${meta.id}" + if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" """ touch ${prefix}.bam touch ${prefix}.bam.bai diff --git a/modules/nf-core/picard/markduplicates/meta.yml b/modules/nf-core/picard/markduplicates/meta.yml index f7693d2f..1ab90c07 100644 --- a/modules/nf-core/picard/markduplicates/meta.yml +++ b/modules/nf-core/picard/markduplicates/meta.yml @@ -69,3 +69,7 @@ authors: - "@drpatelh" - "@projectoriented" - "@ramprasadn" +maintainers: + - "@drpatelh" + - "@projectoriented" + - "@ramprasadn" diff --git a/modules/nf-core/picard/renamesampleinvcf/main.nf b/modules/nf-core/picard/renamesampleinvcf/main.nf index 75dd64b7..d44b1829 100644 --- a/modules/nf-core/picard/renamesampleinvcf/main.nf +++ b/modules/nf-core/picard/renamesampleinvcf/main.nf @@ -3,10 +3,10 @@ process PICARD_RENAMESAMPLEINVCF { tag "$meta.id" label 'process_single' - conda "bioconda::picard=3.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : - 'biocontainers/picard:3.0.0--hdfd78af_1' }" + 'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' : + 'biocontainers/picard:3.1.1--hdfd78af_0' }" input: tuple val(meta), path(vcf) diff --git a/modules/nf-core/picard/renamesampleinvcf/meta.yml b/modules/nf-core/picard/renamesampleinvcf/meta.yml index ac678983..528002d0 100644 --- a/modules/nf-core/picard/renamesampleinvcf/meta.yml +++ b/modules/nf-core/picard/renamesampleinvcf/meta.yml @@ -3,6 +3,7 @@ description: changes name of sample in the vcf file keywords: - picard - picard/renamesampleinvcf + - vcf tools: - "picard": description: | @@ -11,9 +12,7 @@ tools: homepage: https://broadinstitute.github.io/picard/ documentation: https://broadinstitute.github.io/picard/ tool_dev_url: "https://github.com/broadinstitute/picard" - - licence: "['MIT']" - + licence: ["MIT"] input: - meta: type: map @@ -24,7 +23,6 @@ input: type: file description: VCF file pattern: "*.{vcf,vcf.gz}" - output: - meta: type: map @@ -39,6 +37,7 @@ output: type: file description: VCF file pattern: "*.{vcf,vcf.gz}" - authors: - "@Lucpen" +maintainers: + - "@Lucpen" diff --git a/modules/nf-core/picard/sortvcf/main.nf b/modules/nf-core/picard/sortvcf/main.nf index b8b1f833..5359caae 100644 --- a/modules/nf-core/picard/sortvcf/main.nf +++ b/modules/nf-core/picard/sortvcf/main.nf @@ -2,10 +2,10 @@ process PICARD_SORTVCF { tag "$meta.id" label 'process_medium' - conda "bioconda::picard=3.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : - 'biocontainers/picard:3.0.0--hdfd78af_1' }" + 'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' : + 'biocontainers/picard:3.1.1--hdfd78af_0' }" input: tuple val(meta), path(vcf) diff --git a/modules/nf-core/picard/sortvcf/meta.yml b/modules/nf-core/picard/sortvcf/meta.yml index 0a19784d..62507a08 100644 --- a/modules/nf-core/picard/sortvcf/meta.yml +++ b/modules/nf-core/picard/sortvcf/meta.yml @@ -10,7 +10,6 @@ tools: homepage: https://broadinstitute.github.io/picard/ documentation: https://broadinstitute.github.io/picard/command-line-overview.html#SortVcf licence: ["MIT"] - input: - meta: type: map @@ -39,7 +38,6 @@ input: type: file description: Reference genome dictionary file pattern: "*.{dict}" - output: - meta: type: map @@ -54,6 +52,7 @@ output: type: file description: Sorted VCF file pattern: "*.{vcf}" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/qualimap/bamqc/main.nf b/modules/nf-core/qualimap/bamqc/main.nf index fef7307a..8140e143 100644 --- a/modules/nf-core/qualimap/bamqc/main.nf +++ b/modules/nf-core/qualimap/bamqc/main.nf @@ -2,10 +2,10 @@ process QUALIMAP_BAMQC { tag "$meta.id" label 'process_medium' - conda "bioconda::qualimap=2.2.2d" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/qualimap:2.2.2d--1' : - 'biocontainers/qualimap:2.2.2d--1' }" + 'https://depot.galaxyproject.org/singularity/qualimap:2.3--hdfd78af_0' : + 'biocontainers/qualimap:2.3--hdfd78af_0' }" input: tuple val(meta), path(bam) diff --git a/modules/nf-core/qualimap/bamqc/meta.yml b/modules/nf-core/qualimap/bamqc/meta.yml index 303532eb..7756d497 100644 --- a/modules/nf-core/qualimap/bamqc/meta.yml +++ b/modules/nf-core/qualimap/bamqc/meta.yml @@ -36,7 +36,7 @@ output: Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - results: - type: dir + type: directory description: Qualimap results dir pattern: "*/*" - versions: @@ -45,3 +45,5 @@ output: pattern: "versions.yml" authors: - "@phue" +maintainers: + - "@phue" diff --git a/modules/nf-core/rhocall/annotate/main.nf b/modules/nf-core/rhocall/annotate/main.nf index a55578ab..78de45c1 100644 --- a/modules/nf-core/rhocall/annotate/main.nf +++ b/modules/nf-core/rhocall/annotate/main.nf @@ -2,7 +2,7 @@ process RHOCALL_ANNOTATE { tag "$meta.id" label 'process_medium' - conda "bioconda::rhocall=0.5.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/rhocall:0.5.1--py39hbf8eff0_0': 'biocontainers/rhocall:0.5.1--py39hbf8eff0_0' }" diff --git a/modules/nf-core/rhocall/annotate/meta.yml b/modules/nf-core/rhocall/annotate/meta.yml index 96e10d96..ecc8e1e4 100644 --- a/modules/nf-core/rhocall/annotate/meta.yml +++ b/modules/nf-core/rhocall/annotate/meta.yml @@ -10,9 +10,7 @@ tools: homepage: "https://github.com/dnil/rhocall" documentation: "https://github.com/dnil/rhocall" tool_dev_url: "https://github.com/dnil" - licence: "['GPL v3']" - input: - meta: type: map @@ -40,7 +38,6 @@ input: type: file description: BED file with AZ windows. pattern: "*.{bed}" - output: - meta: type: map @@ -55,6 +52,7 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf index 59ed3088..d3461627 100644 --- a/modules/nf-core/samtools/faidx/main.nf +++ b/modules/nf-core/samtools/faidx/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_FAIDX { tag "$fasta" label 'process_single' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml index 957b25e5..e189af28 100644 --- a/modules/nf-core/samtools/faidx/meta.yml +++ b/modules/nf-core/samtools/faidx/meta.yml @@ -55,3 +55,7 @@ authors: - "@drpatelh" - "@ewels" - "@phue" +maintainers: + - "@drpatelh" + - "@ewels" + - "@phue" diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf index 0b20aa4b..8ad18fdc 100644 --- a/modules/nf-core/samtools/index/main.nf +++ b/modules/nf-core/samtools/index/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_INDEX { tag "$meta.id" label 'process_low' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" input: tuple val(meta), path(input) diff --git a/modules/nf-core/samtools/index/meta.yml b/modules/nf-core/samtools/index/meta.yml index 8bd2fa6f..01a4ee03 100644 --- a/modules/nf-core/samtools/index/meta.yml +++ b/modules/nf-core/samtools/index/meta.yml @@ -51,3 +51,7 @@ authors: - "@drpatelh" - "@ewels" - "@maxulysse" +maintainers: + - "@drpatelh" + - "@ewels" + - "@maxulysse" diff --git a/modules/nf-core/samtools/merge/main.nf b/modules/nf-core/samtools/merge/main.nf index b73b7cb2..e104b90a 100644 --- a/modules/nf-core/samtools/merge/main.nf +++ b/modules/nf-core/samtools/merge/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_MERGE { tag "$meta.id" label 'process_low' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" input: tuple val(meta), path(input_files, stageAs: "?/*") @@ -16,6 +16,7 @@ process SAMTOOLS_MERGE { tuple val(meta), path("${prefix}.bam") , optional:true, emit: bam tuple val(meta), path("${prefix}.cram"), optional:true, emit: cram tuple val(meta), path("*.csi") , optional:true, emit: csi + tuple val(meta), path("*.crai") , optional:true, emit: crai path "versions.yml" , emit: versions @@ -43,10 +44,14 @@ process SAMTOOLS_MERGE { """ stub: + def args = task.ext.args ?: '' prefix = task.ext.suffix ? "${meta.id}${task.ext.suffix}" : "${meta.id}" def file_type = input_files instanceof List ? input_files[0].getExtension() : input_files.getExtension() + def index_type = file_type == "bam" ? "csi" : "crai" + def index = args.contains("--write-index") ? "touch ${prefix}.${index_type}" : "" """ touch ${prefix}.${file_type} + ${index} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/samtools/merge/meta.yml b/modules/nf-core/samtools/merge/meta.yml index 3a815f74..2e8f3dbb 100644 --- a/modules/nf-core/samtools/merge/meta.yml +++ b/modules/nf-core/samtools/merge/meta.yml @@ -65,9 +65,19 @@ output: type: file description: BAM index file (optional) pattern: "*.csi" + - crai: + type: file + description: CRAM index file (optional) + pattern: "*.crai" authors: - "@drpatelh" - "@yuukiiwa " - "@maxulysse" - "@FriederikeHanssen" - "@ramprasadn" +maintainers: + - "@drpatelh" + - "@yuukiiwa " + - "@maxulysse" + - "@FriederikeHanssen" + - "@ramprasadn" diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf index 2b7753fd..4a666d42 100644 --- a/modules/nf-core/samtools/sort/main.nf +++ b/modules/nf-core/samtools/sort/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_SORT { tag "$meta.id" label 'process_medium' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" input: tuple val(meta), path(bam) diff --git a/modules/nf-core/samtools/sort/meta.yml b/modules/nf-core/samtools/sort/meta.yml index 07328431..2200de72 100644 --- a/modules/nf-core/samtools/sort/meta.yml +++ b/modules/nf-core/samtools/sort/meta.yml @@ -46,3 +46,6 @@ output: authors: - "@drpatelh" - "@ewels" +maintainers: + - "@drpatelh" + - "@ewels" diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf index 4a2607de..7539140a 100644 --- a/modules/nf-core/samtools/stats/main.nf +++ b/modules/nf-core/samtools/stats/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_STATS { tag "$meta.id" label 'process_single' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" input: tuple val(meta), path(input), path(input_index) diff --git a/modules/nf-core/samtools/stats/meta.yml b/modules/nf-core/samtools/stats/meta.yml index 90e6345f..735ff812 100644 --- a/modules/nf-core/samtools/stats/meta.yml +++ b/modules/nf-core/samtools/stats/meta.yml @@ -57,3 +57,7 @@ authors: - "@drpatelh" - "@FriederikeHanssen" - "@ramprasadn" +maintainers: + - "@drpatelh" + - "@FriederikeHanssen" + - "@ramprasadn" diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf index cb91facf..0b5a2912 100644 --- a/modules/nf-core/samtools/view/main.nf +++ b/modules/nf-core/samtools/view/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_VIEW { tag "$meta.id" label 'process_low' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" input: tuple val(meta), path(input), path(index) @@ -53,10 +53,19 @@ process SAMTOOLS_VIEW { """ stub: + def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + def file_type = args.contains("--output-fmt sam") ? "sam" : + args.contains("--output-fmt bam") ? "bam" : + args.contains("--output-fmt cram") ? "cram" : + input.getExtension() + if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + + def index = args.contains("--write-index") ? "touch ${prefix}.csi" : "" + """ - touch ${prefix}.bam - touch ${prefix}.cram + touch ${prefix}.${file_type} + ${index} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/samtools/view/meta.yml b/modules/nf-core/samtools/view/meta.yml index 3b05450b..3dadafae 100644 --- a/modules/nf-core/samtools/view/meta.yml +++ b/modules/nf-core/samtools/view/meta.yml @@ -82,3 +82,8 @@ authors: - "@joseespinosa" - "@FriederikeHanssen" - "@priyanka-surana" +maintainers: + - "@drpatelh" + - "@joseespinosa" + - "@FriederikeHanssen" + - "@priyanka-surana" diff --git a/modules/nf-core/sentieon/bwaindex/main.nf b/modules/nf-core/sentieon/bwaindex/main.nf index 38e1d3a3..0d99cb67 100644 --- a/modules/nf-core/sentieon/bwaindex/main.nf +++ b/modules/nf-core/sentieon/bwaindex/main.nf @@ -3,7 +3,10 @@ process SENTIEON_BWAINDEX { label 'process_high' label 'sentieon' - container 'nf-core/sentieon:202112.06' + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' : + 'biocontainers/sentieon:202308.01--h43eeafb_0' }" input: tuple val(meta), path(fasta) @@ -16,13 +19,20 @@ process SENTIEON_BWAINDEX { task.ext.when == null || task.ext.when script: - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' } + def args = task.ext.args ?: '' def prefix = task.ext.prefix ? "bwa/${task.ext.prefix}" : "bwa/${fasta.baseName}" """ + $fix_ld_library_path + mkdir bwa sentieon \\ @@ -39,11 +49,18 @@ process SENTIEON_BWAINDEX { """ stub: - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' } + """ + $fix_ld_library_path + mkdir bwa touch bwa/genome.amb diff --git a/modules/nf-core/sentieon/bwaindex/meta.yml b/modules/nf-core/sentieon/bwaindex/meta.yml index 5d91e225..0af45ad8 100644 --- a/modules/nf-core/sentieon/bwaindex/meta.yml +++ b/modules/nf-core/sentieon/bwaindex/meta.yml @@ -39,3 +39,6 @@ output: authors: - "@drpatelh" - "@maxulysse" +maintainers: + - "@drpatelh" + - "@maxulysse" diff --git a/modules/nf-core/sentieon/bwamem/main.nf b/modules/nf-core/sentieon/bwamem/main.nf index b58835fd..e25515e7 100644 --- a/modules/nf-core/sentieon/bwamem/main.nf +++ b/modules/nf-core/sentieon/bwamem/main.nf @@ -5,7 +5,10 @@ process SENTIEON_BWAMEM { secret 'SENTIEON_LICENSE_BASE64' - container 'nf-core/sentieon:202112.06' + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' : + 'biocontainers/sentieon:202308.01--h43eeafb_0' }" input: tuple val(meta), path(reads) @@ -21,10 +24,15 @@ process SENTIEON_BWAMEM { task.ext.when == null || task.ext.when script: - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' } + def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: '' @@ -47,6 +55,8 @@ process SENTIEON_BWAMEM { echo "Decoded and exported Sentieon test-license system environment variables" fi + $fix_ld_library_path + INDEX=`find -L ./ -name "*.amb" | sed 's/.amb//'` sentieon bwa mem \\ @@ -64,8 +74,19 @@ process SENTIEON_BWAMEM { """ stub: + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' + } + def prefix = task.ext.prefix ?: "${meta.id}" """ + $fix_ld_library_path + touch ${prefix}.bam touch ${prefix}.bam.bai diff --git a/modules/nf-core/sentieon/bwamem/meta.yml b/modules/nf-core/sentieon/bwamem/meta.yml index 9987e8c9..0859a923 100644 --- a/modules/nf-core/sentieon/bwamem/meta.yml +++ b/modules/nf-core/sentieon/bwamem/meta.yml @@ -71,3 +71,5 @@ output: pattern: "versions.yml" authors: - "@asp8200" +maintainers: + - "@asp8200" diff --git a/modules/nf-core/sentieon/datametrics/main.nf b/modules/nf-core/sentieon/datametrics/main.nf index 831a8f68..3be2d11a 100644 --- a/modules/nf-core/sentieon/datametrics/main.nf +++ b/modules/nf-core/sentieon/datametrics/main.nf @@ -5,7 +5,10 @@ process SENTIEON_DATAMETRICS { secret 'SENTIEON_LICENSE_BASE64' - container 'nf-core/sentieon:202112.06' + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' : + 'biocontainers/sentieon:202308.01--h43eeafb_0' }" input: tuple val(meta), path(bam), path(bai) @@ -25,10 +28,15 @@ process SENTIEON_DATAMETRICS { task.ext.when == null || task.ext.when script: - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' } + def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def input = bam.sort().collect{"-i $it"}.join(' ') @@ -51,6 +59,8 @@ process SENTIEON_DATAMETRICS { echo "Decoded and exported Sentieon test-license system environment variables" fi + $fix_ld_library_path + sentieon \\ driver \\ -t $task.cpus \\ @@ -70,8 +80,19 @@ process SENTIEON_DATAMETRICS { """ stub: + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' + } + def prefix = task.ext.prefix ?: "${meta.id}" """ + $fix_ld_library_path + touch ${prefix}_mq_metrics.txt touch ${prefix}_qd_metrics.txt touch ${prefix}_gc_summary.txt diff --git a/modules/nf-core/sentieon/datametrics/meta.yml b/modules/nf-core/sentieon/datametrics/meta.yml index bac58b9a..d2d1450a 100644 --- a/modules/nf-core/sentieon/datametrics/meta.yml +++ b/modules/nf-core/sentieon/datametrics/meta.yml @@ -1,4 +1,4 @@ -name: "sentieon_datametrics" +name: sentieon_datametrics description: Collects multiple quality metrics from a bam file keywords: - metrics @@ -11,7 +11,6 @@ tools: Our software improves upon BWA, STAR, Minimap2, GATK, HaplotypeCaller, Mutect, and Mutect2 based pipelines and is deployable on any generic-CPU-based computing system. homepage: https://www.sentieon.com/ documentation: https://www.sentieon.com/ - input: - meta: type: map @@ -44,7 +43,6 @@ input: type: file description: Index of the genome fasta file pattern: "*.fai" - output: - meta: type: map @@ -79,6 +77,7 @@ output: type: file description: File containing the information about statistical distribution of insert sizes pattern: "*.txt" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/sentieon/dedup/main.nf b/modules/nf-core/sentieon/dedup/main.nf index c83d5e55..316993cc 100644 --- a/modules/nf-core/sentieon/dedup/main.nf +++ b/modules/nf-core/sentieon/dedup/main.nf @@ -5,7 +5,10 @@ process SENTIEON_DEDUP { secret 'SENTIEON_LICENSE_BASE64' - container 'nf-core/sentieon:202112.06' + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' : + 'biocontainers/sentieon:202308.01--h43eeafb_0' }" input: tuple val(meta), path(bam), path(bai) @@ -26,10 +29,15 @@ process SENTIEON_DEDUP { task.ext.when == null || task.ext.when script: - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' } + def args = task.ext.args ?: '' def args2 = task.ext.args2 ?: '' def args3 = task.ext.args3 ?: '' @@ -58,6 +66,8 @@ process SENTIEON_DEDUP { echo "Decoded and exported Sentieon test-license system environment variables" fi + $fix_ld_library_path + sentieon driver $args $input_list -r ${fasta} --algo LocusCollector $args2 --fun score_info ${prefix}.score sentieon driver $args3 -t $task.cpus $input_list -r ${fasta} --algo Dedup $args4 --score_info ${prefix}.score --metrics ${metrics} ${prefix}${suffix} # This following tsv-file is produced in order to get a proper tsv-file with Dedup-metrics for importing in MultiQC as "custom content". @@ -71,12 +81,25 @@ process SENTIEON_DEDUP { """ stub: + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' + } def prefix = task.ext.prefix ?: "${meta.id}" + def suffix = task.ext.suffix ?: ".cram" // The suffix should be either ".cram" or ".bam". + def metrics = task.ext.metrics ?: "${prefix}${suffix}.metrics" """ - touch ${prefix}.cram - touch ${prefix}.cram.crai - touch ${prefix}.metrics - touch ${prefix}.score + $fix_ld_library_path + + touch "${prefix}${suffix}" + touch "${prefix}${suffix}\$(echo ${suffix} | sed 's/m\$/i/')" + touch "${metrics}" + touch "${metrics}.multiqc.tsv" + touch "${prefix}.score" cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/sentieon/dedup/meta.yml b/modules/nf-core/sentieon/dedup/meta.yml index ec0565d9..0efbb96c 100644 --- a/modules/nf-core/sentieon/dedup/meta.yml +++ b/modules/nf-core/sentieon/dedup/meta.yml @@ -86,3 +86,5 @@ output: pattern: "versions.yml" authors: - "@asp8200" +maintainers: + - "@asp8200" diff --git a/modules/nf-core/sentieon/dnamodelapply/main.nf b/modules/nf-core/sentieon/dnamodelapply/main.nf index 3fe9a28f..1cbb02e3 100644 --- a/modules/nf-core/sentieon/dnamodelapply/main.nf +++ b/modules/nf-core/sentieon/dnamodelapply/main.nf @@ -5,7 +5,10 @@ process SENTIEON_DNAMODELAPPLY { secret 'SENTIEON_LICENSE_BASE64' - container 'nf-core/sentieon:202112.06' + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' : + 'biocontainers/sentieon:202308.01--h43eeafb_0' }" input: tuple val(meta), path(vcf), path(idx) @@ -22,10 +25,15 @@ process SENTIEON_DNAMODELAPPLY { task.ext.when == null || task.ext.when script: - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' } + def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: '' @@ -48,6 +56,8 @@ process SENTIEON_DNAMODELAPPLY { echo "Decoded and exported Sentieon test-license system environment variables" fi + $fix_ld_library_path + sentieon driver \\ -t $task.cpus \\ -r $fasta \\ @@ -64,12 +74,20 @@ process SENTIEON_DNAMODELAPPLY { """ stub: - def prefix = task.ext.prefix ?: "${meta.id}" - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' } + + def prefix = task.ext.prefix ?: "${meta.id}" + """ + $fix_ld_library_path + touch ${prefix}.vcf.gz touch ${prefix}.vcf.gz.tbi diff --git a/modules/nf-core/sentieon/dnamodelapply/meta.yml b/modules/nf-core/sentieon/dnamodelapply/meta.yml index ec429bea..25076545 100644 --- a/modules/nf-core/sentieon/dnamodelapply/meta.yml +++ b/modules/nf-core/sentieon/dnamodelapply/meta.yml @@ -1,4 +1,4 @@ -name: "sentieon_dnamodelapply" +name: sentieon_dnamodelapply description: modifies the input VCF file by adding the MLrejected FILTER to the variants keywords: - dnamodelapply @@ -12,7 +12,6 @@ tools: Our software improves upon BWA, STAR, Minimap2, GATK, HaplotypeCaller, Mutect, and Mutect2 based pipelines and is deployable on any generic-CPU-based computing system. homepage: https://www.sentieon.com/ documentation: https://www.sentieon.com/ - input: - meta: type: map @@ -54,7 +53,6 @@ input: type: file description: machine learning model file pattern: "*.model" - output: - meta: type: map @@ -73,6 +71,7 @@ output: type: file description: Index of the input VCF file pattern: "*.{tbi}" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/sentieon/dnascope/main.nf b/modules/nf-core/sentieon/dnascope/main.nf index 6be42a17..7e5adefc 100644 --- a/modules/nf-core/sentieon/dnascope/main.nf +++ b/modules/nf-core/sentieon/dnascope/main.nf @@ -5,7 +5,10 @@ process SENTIEON_DNASCOPE { secret 'SENTIEON_LICENSE_BASE64' - container 'nf-core/sentieon:202112.06' + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' : + 'biocontainers/sentieon:202308.01--h43eeafb_0' }" input: tuple val(meta), path(bam), path(bai), path(intervals) @@ -29,10 +32,15 @@ process SENTIEON_DNASCOPE { task.ext.when == null || task.ext.when script: - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' } + def args = task.ext.args ?: '' // options for the driver def args2 = task.ext.args2 ?: '' // options for the vcf generation def args3 = task.ext.args3 ?: '' // options for the gvcf generation @@ -72,6 +80,8 @@ process SENTIEON_DNASCOPE { echo "Decoded and exported Sentieon test-license system environment variables" fi + $fix_ld_library_path + sentieon driver $args -r $fasta -t $task.cpus -i $bam $interval $vcf_cmd $gvcf_cmd cat <<-END_VERSIONS > versions.yml @@ -81,12 +91,20 @@ process SENTIEON_DNASCOPE { """ stub: - def prefix = task.ext.prefix ?: "${meta.id}" - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' } + + def prefix = task.ext.prefix ?: "${meta.id}" + """ + $fix_ld_library_path + touch ${prefix}.unfiltered.vcf.gz touch ${prefix}.unfiltered.vcf.gz.tbi touch ${prefix}.g.vcf.gz diff --git a/modules/nf-core/sentieon/dnascope/meta.yml b/modules/nf-core/sentieon/dnascope/meta.yml index 34e0b97b..6b61cee8 100644 --- a/modules/nf-core/sentieon/dnascope/meta.yml +++ b/modules/nf-core/sentieon/dnascope/meta.yml @@ -114,6 +114,7 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/sentieon/readwriter/main.nf b/modules/nf-core/sentieon/readwriter/main.nf index 080b3ad7..b9afe576 100644 --- a/modules/nf-core/sentieon/readwriter/main.nf +++ b/modules/nf-core/sentieon/readwriter/main.nf @@ -5,7 +5,10 @@ process SENTIEON_READWRITER { secret 'SENTIEON_LICENSE_BASE64' - container 'nf-core/sentieon:202112.06' + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' : + 'biocontainers/sentieon:202308.01--h43eeafb_0' }" input: tuple val(meta), path(input), path(index) @@ -22,10 +25,15 @@ process SENTIEON_READWRITER { task.ext.when == null || task.ext.when script: - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' } + def args = task.ext.args ?: '' def args2 = task.ext.args2 ?: '' def input_str = input.sort().collect{"-i $it"}.join(' ') @@ -52,6 +60,8 @@ process SENTIEON_READWRITER { echo "Decoded and exported Sentieon test-license system environment variables" fi + $fix_ld_library_path + sentieon \\ driver \\ -t $task.cpus \\ @@ -69,10 +79,21 @@ process SENTIEON_READWRITER { """ stub: + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' + } + def prefix = task.ext.prefix ?: "${meta.id}" format = input.extension == "bam" ? "bam" : "cram" index = format == "bam" ? "bam.bai" : "cram.crai" """ + $fix_ld_library_path + touch ${prefix}.${format} touch ${prefix}.${index} diff --git a/modules/nf-core/sentieon/readwriter/meta.yml b/modules/nf-core/sentieon/readwriter/meta.yml index 23dcfcab..d2bbec28 100644 --- a/modules/nf-core/sentieon/readwriter/meta.yml +++ b/modules/nf-core/sentieon/readwriter/meta.yml @@ -44,7 +44,6 @@ input: type: file description: The index of the FASTA reference. pattern: "*.fai" - output: - meta: type: map @@ -66,6 +65,7 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/sentieon/wgsmetrics/main.nf b/modules/nf-core/sentieon/wgsmetrics/main.nf index fd7fdae5..b16641d9 100644 --- a/modules/nf-core/sentieon/wgsmetrics/main.nf +++ b/modules/nf-core/sentieon/wgsmetrics/main.nf @@ -5,7 +5,10 @@ process SENTIEON_WGSMETRICS { secret 'SENTIEON_LICENSE_BASE64' - container 'nf-core/sentieon:202112.06' + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' : + 'biocontainers/sentieon:202308.01--h43eeafb_0' }" input: tuple val(meta), path(bam), path(bai) @@ -22,10 +25,15 @@ process SENTIEON_WGSMETRICS { task.ext.when == null || task.ext.when script: - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' } + def args = task.ext.args ?: '' def input = bam.sort().collect{"-i $it"}.join(' ') def prefix = task.ext.prefix ?: "${meta.id}" @@ -49,6 +57,8 @@ process SENTIEON_WGSMETRICS { echo "Decoded and exported Sentieon test-license system environment variables" fi + $fix_ld_library_path + sentieon \\ driver \\ -t $task.cpus \\ @@ -65,12 +75,19 @@ process SENTIEON_WGSMETRICS { """ stub: - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead." + // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. + // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. + // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. + if (workflow.containerEngine == 'singularity') { + fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' + } else { + fix_ld_library_path = '' } + def prefix = task.ext.prefix ?: "${meta.id}" """ + $fix_ld_library_path + touch ${prefix}.txt cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/sentieon/wgsmetrics/meta.yml b/modules/nf-core/sentieon/wgsmetrics/meta.yml index 09fb9eca..86431e1c 100644 --- a/modules/nf-core/sentieon/wgsmetrics/meta.yml +++ b/modules/nf-core/sentieon/wgsmetrics/meta.yml @@ -1,4 +1,4 @@ -name: "sentieon_wgsmetrics" +name: sentieon_wgsmetrics description: Collects whole genome quality metrics from a bam file keywords: - metrics @@ -11,7 +11,6 @@ tools: Our software improves upon BWA, STAR, Minimap2, GATK, HaplotypeCaller, Mutect, and Mutect2 based pipelines and is deployable on any generic-CPU-based computing system. homepage: https://www.sentieon.com/ documentation: https://www.sentieon.com/ - input: - meta: type: map @@ -53,7 +52,6 @@ input: type: file description: bed or interval_list file containing interval in the reference that will be used in the analysis pattern: "*.{bed,interval_list}" - output: - meta: type: map @@ -68,6 +66,7 @@ output: type: file description: File containing the information about mean base quality score for each sequencing cycle pattern: "*.txt" - authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/smncopynumbercaller/main.nf b/modules/nf-core/smncopynumbercaller/main.nf index 15abea2c..efe0a639 100644 --- a/modules/nf-core/smncopynumbercaller/main.nf +++ b/modules/nf-core/smncopynumbercaller/main.nf @@ -2,7 +2,7 @@ process SMNCOPYNUMBERCALLER { tag "$meta.id" label 'process_low' - conda "bioconda::smncopynumbercaller=1.1.2" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/smncopynumbercaller:1.1.2--py310h7cba7a3_0' : 'biocontainers/smncopynumbercaller:1.1.2--py310h7cba7a3_0' }" diff --git a/modules/nf-core/smncopynumbercaller/meta.yml b/modules/nf-core/smncopynumbercaller/meta.yml index 6d4e10b3..bb2690e9 100644 --- a/modules/nf-core/smncopynumbercaller/meta.yml +++ b/modules/nf-core/smncopynumbercaller/meta.yml @@ -10,7 +10,6 @@ tools: tool_dev_url: "https://github.com/Illumina/SMNCopyNumberCaller" doi: "10.1038/s41436-020-0754-0" licence: "Apache License Version 2.0" - input: - bam: type: file @@ -25,7 +24,6 @@ input: description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - output: - meta: type: map @@ -44,6 +42,7 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@peterpru" +maintainers: + - "@peterpru" diff --git a/modules/nf-core/stranger/main.nf b/modules/nf-core/stranger/main.nf index 0c8a3412..dd44bb99 100644 --- a/modules/nf-core/stranger/main.nf +++ b/modules/nf-core/stranger/main.nf @@ -2,7 +2,7 @@ process STRANGER { tag "$meta.id" label 'process_low' - conda "bioconda::stranger=0.8.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/stranger:0.8.1--pyh5e36f6f_0': 'biocontainers/stranger:0.8.1--pyh5e36f6f_0' }" diff --git a/modules/nf-core/stranger/meta.yml b/modules/nf-core/stranger/meta.yml index eefad398..5e0bc0bb 100644 --- a/modules/nf-core/stranger/meta.yml +++ b/modules/nf-core/stranger/meta.yml @@ -13,7 +13,6 @@ tools: tool_dev_url: https://github.com/moonso/stranger doi: "10.5281/zenodo.4548873" licence: ["MIT"] - input: - meta: type: map @@ -33,7 +32,6 @@ input: type: file description: json file with repeat expansion sites to genotype pattern: "*.{json}" - output: - meta: type: map @@ -48,6 +46,7 @@ output: type: file description: annotated VCF with keys STR_STATUS, NormalMax and PathologicMin pattern: "*.{vcf.gz}" - authors: - "@ljmesi" +maintainers: + - "@ljmesi" diff --git a/modules/nf-core/svdb/merge/main.nf b/modules/nf-core/svdb/merge/main.nf index 0bd94499..0d9967dd 100644 --- a/modules/nf-core/svdb/merge/main.nf +++ b/modules/nf-core/svdb/merge/main.nf @@ -1,7 +1,7 @@ process SVDB_MERGE { tag "$meta.id" label 'process_medium' - conda "bioconda::svdb=2.8.1 bioconda::samtools=1.16.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-c8daa8f9d69d3c5a1a4ff08283a166c18edb0000:af6f8534cd538a85ff43a2eae1b52b143e7abd05-0': 'biocontainers/mulled-v2-c8daa8f9d69d3c5a1a4ff08283a166c18edb0000:af6f8534cd538a85ff43a2eae1b52b143e7abd05-0' }" diff --git a/modules/nf-core/svdb/merge/meta.yml b/modules/nf-core/svdb/merge/meta.yml index 92a5a128..84265acb 100644 --- a/modules/nf-core/svdb/merge/meta.yml +++ b/modules/nf-core/svdb/merge/meta.yml @@ -39,3 +39,5 @@ output: pattern: "*_sv_merge.vcf.gz" authors: - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/tabix/bgzip/main.nf b/modules/nf-core/tabix/bgzip/main.nf index 8c47d9e2..7772e9ad 100644 --- a/modules/nf-core/tabix/bgzip/main.nf +++ b/modules/nf-core/tabix/bgzip/main.nf @@ -2,7 +2,7 @@ process TABIX_BGZIP { tag "$meta.id" label 'process_single' - conda "bioconda::tabix=1.11" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : 'biocontainers/tabix:1.11--hdfd78af_0' }" diff --git a/modules/nf-core/tabix/bgzip/meta.yml b/modules/nf-core/tabix/bgzip/meta.yml index c3ea2107..621d49ea 100644 --- a/modules/nf-core/tabix/bgzip/meta.yml +++ b/modules/nf-core/tabix/bgzip/meta.yml @@ -45,3 +45,8 @@ authors: - "@drpatelh" - "@maxulysse" - "@nvnieuwk" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@maxulysse" + - "@nvnieuwk" diff --git a/modules/nf-core/tabix/bgziptabix/main.nf b/modules/nf-core/tabix/bgziptabix/main.nf index d6c5a760..f9482690 100644 --- a/modules/nf-core/tabix/bgziptabix/main.nf +++ b/modules/nf-core/tabix/bgziptabix/main.nf @@ -2,7 +2,7 @@ process TABIX_BGZIPTABIX { tag "$meta.id" label 'process_single' - conda "bioconda::tabix=1.11" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : 'biocontainers/tabix:1.11--hdfd78af_0' }" diff --git a/modules/nf-core/tabix/bgziptabix/meta.yml b/modules/nf-core/tabix/bgziptabix/meta.yml index 2761e271..438aba4d 100644 --- a/modules/nf-core/tabix/bgziptabix/meta.yml +++ b/modules/nf-core/tabix/bgziptabix/meta.yml @@ -48,3 +48,6 @@ output: authors: - "@maxulysse" - "@DLBPointon" +maintainers: + - "@maxulysse" + - "@DLBPointon" diff --git a/modules/nf-core/tabix/tabix/main.nf b/modules/nf-core/tabix/tabix/main.nf index 5bf332ef..c304a8a3 100644 --- a/modules/nf-core/tabix/tabix/main.nf +++ b/modules/nf-core/tabix/tabix/main.nf @@ -2,7 +2,7 @@ process TABIX_TABIX { tag "$meta.id" label 'process_single' - conda "bioconda::tabix=1.11" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : 'biocontainers/tabix:1.11--hdfd78af_0' }" diff --git a/modules/nf-core/tabix/tabix/meta.yml b/modules/nf-core/tabix/tabix/meta.yml index fcc6e524..ae5b4f43 100644 --- a/modules/nf-core/tabix/tabix/meta.yml +++ b/modules/nf-core/tabix/tabix/meta.yml @@ -43,3 +43,7 @@ authors: - "@joseespinosa" - "@drpatelh" - "@maxulysse" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@maxulysse" diff --git a/modules/nf-core/tiddit/sv/main.nf b/modules/nf-core/tiddit/sv/main.nf index 67a0670d..0f4bc7cb 100644 --- a/modules/nf-core/tiddit/sv/main.nf +++ b/modules/nf-core/tiddit/sv/main.nf @@ -2,7 +2,7 @@ process TIDDIT_SV { tag "$meta.id" label 'process_medium' - conda "bioconda::tiddit=3.6.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/tiddit:3.6.1--py38h24c8ff8_0' : 'biocontainers/tiddit:3.6.1--py38h24c8ff8_0' }" diff --git a/modules/nf-core/tiddit/sv/meta.yml b/modules/nf-core/tiddit/sv/meta.yml index 8b41c69c..b13ae5cd 100644 --- a/modules/nf-core/tiddit/sv/meta.yml +++ b/modules/nf-core/tiddit/sv/meta.yml @@ -53,3 +53,5 @@ output: pattern: "versions.yml" authors: - "@maxulysse" +maintainers: + - "@maxulysse" diff --git a/modules/nf-core/ucsc/wigtobigwig/main.nf b/modules/nf-core/ucsc/wigtobigwig/main.nf index c5f215b1..4b7adb62 100644 --- a/modules/nf-core/ucsc/wigtobigwig/main.nf +++ b/modules/nf-core/ucsc/wigtobigwig/main.nf @@ -3,7 +3,7 @@ process UCSC_WIGTOBIGWIG { label 'process_single' // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. - conda "bioconda::ucsc-wigtobigwig=447" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ucsc-wigtobigwig:447--h2a80c09_1' : 'biocontainers/ucsc-wigtobigwig:447--h2a80c09_1' }" diff --git a/modules/nf-core/ucsc/wigtobigwig/meta.yml b/modules/nf-core/ucsc/wigtobigwig/meta.yml index 470967db..f7476480 100644 --- a/modules/nf-core/ucsc/wigtobigwig/meta.yml +++ b/modules/nf-core/ucsc/wigtobigwig/meta.yml @@ -11,7 +11,6 @@ tools: or bedGraph format) to binary big wig format homepage: http://www.genome.ucsc.edu/goldenPath/help/bigWig.html licence: ["varies; see http://genome.ucsc.edu/license"] - input: - meta: type: map @@ -25,7 +24,6 @@ input: - chromsizes: type: file description: chromosome sizes file - output: - versions: type: file @@ -35,7 +33,9 @@ output: type: file description: bigwig file pattern: "*.{bw}" - authors: - "@jianhong" - "@projectoriented" +maintainers: + - "@jianhong" + - "@projectoriented" diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf index 61461c39..8a75bb95 100644 --- a/modules/nf-core/untar/main.nf +++ b/modules/nf-core/untar/main.nf @@ -2,7 +2,7 @@ process UNTAR { tag "$archive" label 'process_single' - conda "conda-forge::sed=4.7 conda-forge::grep=3.11 conda-forge::tar=1.34" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : 'nf-core/ubuntu:20.04' }" diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/untar/meta.yml index db241a6e..a9a2110f 100644 --- a/modules/nf-core/untar/meta.yml +++ b/modules/nf-core/untar/meta.yml @@ -39,3 +39,8 @@ authors: - "@drpatelh" - "@matthdsm" - "@jfy133" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@matthdsm" + - "@jfy133" diff --git a/modules/nf-core/upd/main.nf b/modules/nf-core/upd/main.nf index a73fcd45..c4bcaf17 100644 --- a/modules/nf-core/upd/main.nf +++ b/modules/nf-core/upd/main.nf @@ -3,7 +3,7 @@ process UPD { tag "$meta.id" label 'process_single' - conda "bioconda::upd=0.1.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/upd:0.1.1--pyhdfd78af_0': 'biocontainers/upd:0.1.1--pyhdfd78af_0' }" diff --git a/modules/nf-core/upd/meta.yml b/modules/nf-core/upd/meta.yml index f99ca01f..7682e337 100644 --- a/modules/nf-core/upd/meta.yml +++ b/modules/nf-core/upd/meta.yml @@ -40,3 +40,5 @@ output: pattern: "*.{bed}" authors: - "@hrydbeck" +maintainers: + - "@hrydbeck" diff --git a/modules/nf-core/vcfanno/main.nf b/modules/nf-core/vcfanno/main.nf index a2a078da..25c131b1 100644 --- a/modules/nf-core/vcfanno/main.nf +++ b/modules/nf-core/vcfanno/main.nf @@ -2,10 +2,10 @@ process VCFANNO { tag "$meta.id" label 'process_low' - conda "bioconda::vcfanno=0.3.3" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/vcfanno:0.3.3--h9ee0642_0': - 'biocontainers/vcfanno:0.3.3--h9ee0642_0' }" + 'https://depot.galaxyproject.org/singularity/vcfanno:0.3.5--h9ee0642_0': + 'biocontainers/vcfanno:0.3.5--h9ee0642_0' }" input: tuple val(meta), path(vcf), path(tbi), path(specific_resources) diff --git a/modules/nf-core/vcfanno/meta.yml b/modules/nf-core/vcfanno/meta.yml index 86fea0c4..89c781ad 100644 --- a/modules/nf-core/vcfanno/meta.yml +++ b/modules/nf-core/vcfanno/meta.yml @@ -10,12 +10,10 @@ keywords: tools: - vcfanno: description: annotate a VCF with other VCFs/BEDs/tabixed files - documentation: https://github.com/brentp/vcfanno#vcfanno tool_dev_url: https://github.com/brentp/vcfanno doi: "10.1186/s13059-016-0973-5" license: ["MIT"] - input: - meta: type: map @@ -44,7 +42,6 @@ input: - resources: type: map description: List of reference files defined in toml config, must also include indices if bgzipped. - output: - meta: type: map @@ -59,7 +56,9 @@ output: type: file description: Annotated VCF file pattern: "*.vcf" - authors: - "@projectoriented" - "@matthdsm" +maintainers: + - "@projectoriented" + - "@matthdsm" From 25980921be607b6b491a0518bc9784e2a18200aa Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 25 Jan 2024 09:55:09 +0100 Subject: [PATCH 269/387] modify RG construction --- workflows/raredisease.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 9481b930..fb7efc55 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -166,7 +166,7 @@ workflow RAREDISEASE { .combine( ch_original_input ) .map { counts, meta, fastq1, fastq2 -> new_meta = meta + [num_lanes:counts[meta.id], - read_group:"\'@RG\\tID:"+ fastq1.toString().split('/')[-1] + "\\tPL:ILLUMINA\\tSM:"+meta.id+"\'"] + read_group:"\'@RG\\tID:"+ fastq1.toString().split('/')[-1] + "\\tPL:" + params.platform.toUpperCase() + "\\tSM:" + meta.id + "\'"] if (!fastq2) { return [ new_meta + [ single_end:true ], [ fastq1 ] ] } else { From 04079e0143f9ca64953d74eee8c68aa53867add0 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 25 Jan 2024 11:09:25 +0100 Subject: [PATCH 270/387] update modules --- .../nf-core/bcftools/annotate/environment.yml | 7 + .../nf-core/bcftools/concat/environment.yml | 7 + .../bcftools/concat/tests/main.nf.test | 108 ++ .../bcftools/concat/tests/main.nf.test.snap | 43 + .../bcftools/concat/tests/nextflow.config | 3 + .../nf-core/bcftools/concat/tests/tags.yml | 2 + .../nf-core/bcftools/filter/environment.yml | 7 + .../nf-core/bcftools/merge/environment.yml | 7 + modules/nf-core/bcftools/norm/environment.yml | 7 + .../nf-core/bcftools/reheader/environment.yml | 7 + .../bcftools/reheader/tests/bcf.config | 4 + .../bcftools/reheader/tests/main.nf.test | 197 +++ .../bcftools/reheader/tests/main.nf.test.snap | 166 +++ .../nf-core/bcftools/reheader/tests/tags.yml | 2 + .../bcftools/reheader/tests/vcf.config | 4 + .../bcftools/reheader/tests/vcf.gz.config | 4 + modules/nf-core/bcftools/roh/environment.yml | 7 + modules/nf-core/bcftools/sort/environment.yml | 7 + modules/nf-core/bcftools/view/environment.yml | 7 + .../nf-core/bcftools/view/tests/main.nf.test | 103 ++ .../bcftools/view/tests/main.nf.test.snap | 45 + .../bcftools/view/tests/nextflow.config | 3 + modules/nf-core/bcftools/view/tests/tags.yml | 2 + modules/nf-core/bwa/index/environment.yml | 7 + modules/nf-core/bwa/index/tests/main.nf.test | 33 + .../nf-core/bwa/index/tests/main.nf.test.snap | 43 + modules/nf-core/bwa/index/tests/tags.yml | 2 + modules/nf-core/bwamem2/index/environment.yml | 7 + modules/nf-core/bwamem2/mem/environment.yml | 9 + .../nf-core/bwamem2/mem/tests/main.nf.test | 213 +++ .../bwamem2/mem/tests/main.nf.test.snap | 47 + modules/nf-core/bwamem2/mem/tests/tags.yml | 2 + modules/nf-core/cadd/environment.yml | 9 + modules/nf-core/chromograph/environment.yml | 7 + .../nf-core/cnvnator/cnvnator/environment.yml | 7 + .../cnvnator/convert2vcf/environment.yml | 7 + modules/nf-core/deepvariant/environment.yml | 5 + .../ensemblvep/filtervep/environment.yml | 7 + .../nf-core/expansionhunter/environment.yml | 7 + .../gatk4/bedtointervallist/environment.yml | 7 + .../gatk4/collectreadcounts/environment.yml | 7 + .../createsequencedictionary/environment.yml | 7 + .../gatk4/denoisereadcounts/environment.yml | 7 + .../environment.yml | 5 + .../gatk4/filtermutectcalls/environment.yml | 7 + .../gatk4/germlinecnvcaller/environment.yml | 5 + .../gatk4/intervallisttools/environment.yml | 7 + .../gatk4/mergebamalignment/environment.yml | 7 + .../nf-core/gatk4/mergevcfs/environment.yml | 7 + modules/nf-core/gatk4/mutect2/environment.yml | 7 + .../environment.yml | 5 + .../gatk4/preprocessintervals/environment.yml | 7 + .../nf-core/gatk4/printreads/environment.yml | 7 + .../nf-core/gatk4/revertsam/environment.yml | 7 + .../nf-core/gatk4/samtofastq/environment.yml | 7 + .../gatk4/selectvariants/environment.yml | 7 + .../nf-core/gatk4/shiftfasta/environment.yml | 7 + .../gatk4/splitintervals/environment.yml | 7 + .../gatk4/variantfiltration/environment.yml | 7 + .../nf-core/genmod/annotate/environment.yml | 7 + .../nf-core/genmod/compound/environment.yml | 7 + modules/nf-core/genmod/models/environment.yml | 8 + modules/nf-core/genmod/score/environment.yml | 7 + modules/nf-core/glnexus/environment.yml | 7 + modules/nf-core/haplocheck/environment.yml | 7 + .../haplogrep2/classify/environment.yml | 7 + .../nf-core/hmtnote/annotate/environment.yml | 7 + .../nf-core/manta/germline/environment.yml | 7 + modules/nf-core/mosdepth/environment.yml | 8 + modules/nf-core/mosdepth/tests/main.nf.test | 260 ++++ .../nf-core/mosdepth/tests/main.nf.test.snap | 1145 +++++++++++++++++ .../nf-core/mosdepth/tests/quantized.config | 3 + modules/nf-core/mosdepth/tests/tags.yml | 2 + .../nf-core/mosdepth/tests/threshold.config | 3 + modules/nf-core/mosdepth/tests/window.config | 3 + .../nf-core/multiqc/tests/main.nf.test.snap | 21 + modules/nf-core/peddy/environment.yml | 7 + .../addorreplacereadgroups/environment.yml | 7 + .../picard/collecthsmetrics/environment.yml | 7 + .../collectmultiplemetrics/environment.yml | 7 + .../picard/collectwgsmetrics/environment.yml | 8 + .../picard/liftovervcf/environment.yml | 7 + .../picard/markduplicates/environment.yml | 7 + .../picard/markduplicates/tests/main.nf.test | 111 ++ .../markduplicates/tests/main.nf.test.snap | 44 + .../markduplicates/tests/nextflow.config | 6 + .../picard/markduplicates/tests/tags.yml | 2 + .../picard/renamesampleinvcf/environment.yml | 7 + .../nf-core/picard/sortvcf/environment.yml | 7 + .../nf-core/qualimap/bamqc/environment.yml | 7 + .../nf-core/qualimap/bamqc/tests/main.nf.test | 39 + .../qualimap/bamqc/tests/main.nf.test.snap | 11 + modules/nf-core/qualimap/bamqc/tests/tags.yml | 2 + .../nf-core/rhocall/annotate/environment.yml | 7 + .../nf-core/samtools/faidx/environment.yml | 7 + .../nf-core/samtools/index/environment.yml | 7 + .../samtools/index/tests/csi.nextflow.config | 7 + .../nf-core/samtools/index/tests/main.nf.test | 87 ++ .../samtools/index/tests/main.nf.test.snap | 28 + modules/nf-core/samtools/index/tests/tags.yml | 2 + .../nf-core/samtools/merge/environment.yml | 7 + .../nf-core/samtools/merge/tests/index.config | 3 + .../nf-core/samtools/merge/tests/main.nf.test | 156 +++ .../samtools/merge/tests/main.nf.test.snap | 68 + modules/nf-core/samtools/merge/tests/tags.yml | 2 + modules/nf-core/samtools/sort/environment.yml | 7 + .../nf-core/samtools/sort/tests/main.nf.test | 73 ++ .../samtools/sort/tests/main.nf.test.snap | 48 + .../samtools/sort/tests/nextflow.config | 7 + modules/nf-core/samtools/sort/tests/tags.yml | 3 + .../nf-core/samtools/stats/environment.yml | 7 + .../nf-core/samtools/stats/tests/main.nf.test | 78 ++ .../samtools/stats/tests/main.nf.test.snap | 64 + modules/nf-core/samtools/stats/tests/tags.yml | 2 + modules/nf-core/samtools/view/environment.yml | 7 + .../nf-core/samtools/view/tests/bam.config | 3 + .../samtools/view/tests/bam_index.config | 3 + .../nf-core/samtools/view/tests/main.nf.test | 231 ++++ .../samtools/view/tests/main.nf.test.snap | 140 ++ modules/nf-core/samtools/view/tests/tags.yml | 2 + .../nf-core/sentieon/bwaindex/environment.yml | 7 + .../nf-core/sentieon/bwamem/environment.yml | 7 + .../sentieon/datametrics/environment.yml | 7 + .../nf-core/sentieon/dedup/environment.yml | 7 + .../sentieon/dnamodelapply/environment.yml | 7 + .../nf-core/sentieon/dnascope/environment.yml | 7 + .../sentieon/readwriter/environment.yml | 7 + .../sentieon/wgsmetrics/environment.yml | 7 + .../smncopynumbercaller/environment.yml | 7 + modules/nf-core/stranger/environment.yml | 7 + modules/nf-core/svdb/merge/environment.yml | 9 + modules/nf-core/tabix/bgzip/environment.yml | 7 + .../nf-core/tabix/bgziptabix/environment.yml | 7 + modules/nf-core/tabix/tabix/environment.yml | 7 + modules/nf-core/tiddit/sv/environment.yml | 7 + .../nf-core/ucsc/wigtobigwig/environment.yml | 7 + modules/nf-core/untar/environment.yml | 11 + modules/nf-core/untar/tests/main.nf.test | 55 + modules/nf-core/untar/tests/main.nf.test.snap | 34 + modules/nf-core/untar/tests/tags.yml | 2 + modules/nf-core/upd/environment.yml | 7 + modules/nf-core/vcfanno/environment.yml | 7 + modules/nf-core/vcfanno/tests/main.nf.test | 106 ++ .../nf-core/vcfanno/tests/main.nf.test.snap | 73 ++ modules/nf-core/vcfanno/tests/nextflow.config | 4 + modules/nf-core/vcfanno/tests/tags.yml | 2 + 146 files changed, 4561 insertions(+) create mode 100644 modules/nf-core/bcftools/annotate/environment.yml create mode 100644 modules/nf-core/bcftools/concat/environment.yml create mode 100644 modules/nf-core/bcftools/concat/tests/main.nf.test create mode 100644 modules/nf-core/bcftools/concat/tests/main.nf.test.snap create mode 100644 modules/nf-core/bcftools/concat/tests/nextflow.config create mode 100644 modules/nf-core/bcftools/concat/tests/tags.yml create mode 100644 modules/nf-core/bcftools/filter/environment.yml create mode 100644 modules/nf-core/bcftools/merge/environment.yml create mode 100644 modules/nf-core/bcftools/norm/environment.yml create mode 100644 modules/nf-core/bcftools/reheader/environment.yml create mode 100644 modules/nf-core/bcftools/reheader/tests/bcf.config create mode 100644 modules/nf-core/bcftools/reheader/tests/main.nf.test create mode 100644 modules/nf-core/bcftools/reheader/tests/main.nf.test.snap create mode 100644 modules/nf-core/bcftools/reheader/tests/tags.yml create mode 100644 modules/nf-core/bcftools/reheader/tests/vcf.config create mode 100644 modules/nf-core/bcftools/reheader/tests/vcf.gz.config create mode 100644 modules/nf-core/bcftools/roh/environment.yml create mode 100644 modules/nf-core/bcftools/sort/environment.yml create mode 100644 modules/nf-core/bcftools/view/environment.yml create mode 100644 modules/nf-core/bcftools/view/tests/main.nf.test create mode 100644 modules/nf-core/bcftools/view/tests/main.nf.test.snap create mode 100644 modules/nf-core/bcftools/view/tests/nextflow.config create mode 100644 modules/nf-core/bcftools/view/tests/tags.yml create mode 100644 modules/nf-core/bwa/index/environment.yml create mode 100644 modules/nf-core/bwa/index/tests/main.nf.test create mode 100644 modules/nf-core/bwa/index/tests/main.nf.test.snap create mode 100644 modules/nf-core/bwa/index/tests/tags.yml create mode 100644 modules/nf-core/bwamem2/index/environment.yml create mode 100644 modules/nf-core/bwamem2/mem/environment.yml create mode 100644 modules/nf-core/bwamem2/mem/tests/main.nf.test create mode 100644 modules/nf-core/bwamem2/mem/tests/main.nf.test.snap create mode 100644 modules/nf-core/bwamem2/mem/tests/tags.yml create mode 100644 modules/nf-core/cadd/environment.yml create mode 100644 modules/nf-core/chromograph/environment.yml create mode 100644 modules/nf-core/cnvnator/cnvnator/environment.yml create mode 100644 modules/nf-core/cnvnator/convert2vcf/environment.yml create mode 100644 modules/nf-core/deepvariant/environment.yml create mode 100644 modules/nf-core/ensemblvep/filtervep/environment.yml create mode 100644 modules/nf-core/expansionhunter/environment.yml create mode 100644 modules/nf-core/gatk4/bedtointervallist/environment.yml create mode 100644 modules/nf-core/gatk4/collectreadcounts/environment.yml create mode 100644 modules/nf-core/gatk4/createsequencedictionary/environment.yml create mode 100644 modules/nf-core/gatk4/denoisereadcounts/environment.yml create mode 100644 modules/nf-core/gatk4/determinegermlinecontigploidy/environment.yml create mode 100644 modules/nf-core/gatk4/filtermutectcalls/environment.yml create mode 100644 modules/nf-core/gatk4/germlinecnvcaller/environment.yml create mode 100644 modules/nf-core/gatk4/intervallisttools/environment.yml create mode 100644 modules/nf-core/gatk4/mergebamalignment/environment.yml create mode 100644 modules/nf-core/gatk4/mergevcfs/environment.yml create mode 100644 modules/nf-core/gatk4/mutect2/environment.yml create mode 100644 modules/nf-core/gatk4/postprocessgermlinecnvcalls/environment.yml create mode 100644 modules/nf-core/gatk4/preprocessintervals/environment.yml create mode 100644 modules/nf-core/gatk4/printreads/environment.yml create mode 100644 modules/nf-core/gatk4/revertsam/environment.yml create mode 100644 modules/nf-core/gatk4/samtofastq/environment.yml create mode 100644 modules/nf-core/gatk4/selectvariants/environment.yml create mode 100644 modules/nf-core/gatk4/shiftfasta/environment.yml create mode 100644 modules/nf-core/gatk4/splitintervals/environment.yml create mode 100644 modules/nf-core/gatk4/variantfiltration/environment.yml create mode 100644 modules/nf-core/genmod/annotate/environment.yml create mode 100644 modules/nf-core/genmod/compound/environment.yml create mode 100644 modules/nf-core/genmod/models/environment.yml create mode 100644 modules/nf-core/genmod/score/environment.yml create mode 100644 modules/nf-core/glnexus/environment.yml create mode 100644 modules/nf-core/haplocheck/environment.yml create mode 100644 modules/nf-core/haplogrep2/classify/environment.yml create mode 100644 modules/nf-core/hmtnote/annotate/environment.yml create mode 100644 modules/nf-core/manta/germline/environment.yml create mode 100644 modules/nf-core/mosdepth/environment.yml create mode 100644 modules/nf-core/mosdepth/tests/main.nf.test create mode 100644 modules/nf-core/mosdepth/tests/main.nf.test.snap create mode 100644 modules/nf-core/mosdepth/tests/quantized.config create mode 100644 modules/nf-core/mosdepth/tests/tags.yml create mode 100644 modules/nf-core/mosdepth/tests/threshold.config create mode 100644 modules/nf-core/mosdepth/tests/window.config create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/peddy/environment.yml create mode 100644 modules/nf-core/picard/addorreplacereadgroups/environment.yml create mode 100644 modules/nf-core/picard/collecthsmetrics/environment.yml create mode 100644 modules/nf-core/picard/collectmultiplemetrics/environment.yml create mode 100644 modules/nf-core/picard/collectwgsmetrics/environment.yml create mode 100644 modules/nf-core/picard/liftovervcf/environment.yml create mode 100644 modules/nf-core/picard/markduplicates/environment.yml create mode 100644 modules/nf-core/picard/markduplicates/tests/main.nf.test create mode 100644 modules/nf-core/picard/markduplicates/tests/main.nf.test.snap create mode 100644 modules/nf-core/picard/markduplicates/tests/nextflow.config create mode 100644 modules/nf-core/picard/markduplicates/tests/tags.yml create mode 100644 modules/nf-core/picard/renamesampleinvcf/environment.yml create mode 100644 modules/nf-core/picard/sortvcf/environment.yml create mode 100644 modules/nf-core/qualimap/bamqc/environment.yml create mode 100644 modules/nf-core/qualimap/bamqc/tests/main.nf.test create mode 100644 modules/nf-core/qualimap/bamqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/qualimap/bamqc/tests/tags.yml create mode 100644 modules/nf-core/rhocall/annotate/environment.yml create mode 100644 modules/nf-core/samtools/faidx/environment.yml create mode 100644 modules/nf-core/samtools/index/environment.yml create mode 100644 modules/nf-core/samtools/index/tests/csi.nextflow.config create mode 100644 modules/nf-core/samtools/index/tests/main.nf.test create mode 100644 modules/nf-core/samtools/index/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/index/tests/tags.yml create mode 100644 modules/nf-core/samtools/merge/environment.yml create mode 100644 modules/nf-core/samtools/merge/tests/index.config create mode 100644 modules/nf-core/samtools/merge/tests/main.nf.test create mode 100644 modules/nf-core/samtools/merge/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/merge/tests/tags.yml create mode 100644 modules/nf-core/samtools/sort/environment.yml create mode 100644 modules/nf-core/samtools/sort/tests/main.nf.test create mode 100644 modules/nf-core/samtools/sort/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/sort/tests/nextflow.config create mode 100644 modules/nf-core/samtools/sort/tests/tags.yml create mode 100644 modules/nf-core/samtools/stats/environment.yml create mode 100644 modules/nf-core/samtools/stats/tests/main.nf.test create mode 100644 modules/nf-core/samtools/stats/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/stats/tests/tags.yml create mode 100644 modules/nf-core/samtools/view/environment.yml create mode 100644 modules/nf-core/samtools/view/tests/bam.config create mode 100644 modules/nf-core/samtools/view/tests/bam_index.config create mode 100644 modules/nf-core/samtools/view/tests/main.nf.test create mode 100644 modules/nf-core/samtools/view/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/view/tests/tags.yml create mode 100644 modules/nf-core/sentieon/bwaindex/environment.yml create mode 100644 modules/nf-core/sentieon/bwamem/environment.yml create mode 100644 modules/nf-core/sentieon/datametrics/environment.yml create mode 100644 modules/nf-core/sentieon/dedup/environment.yml create mode 100644 modules/nf-core/sentieon/dnamodelapply/environment.yml create mode 100644 modules/nf-core/sentieon/dnascope/environment.yml create mode 100644 modules/nf-core/sentieon/readwriter/environment.yml create mode 100644 modules/nf-core/sentieon/wgsmetrics/environment.yml create mode 100644 modules/nf-core/smncopynumbercaller/environment.yml create mode 100644 modules/nf-core/stranger/environment.yml create mode 100644 modules/nf-core/svdb/merge/environment.yml create mode 100644 modules/nf-core/tabix/bgzip/environment.yml create mode 100644 modules/nf-core/tabix/bgziptabix/environment.yml create mode 100644 modules/nf-core/tabix/tabix/environment.yml create mode 100644 modules/nf-core/tiddit/sv/environment.yml create mode 100644 modules/nf-core/ucsc/wigtobigwig/environment.yml create mode 100644 modules/nf-core/untar/environment.yml create mode 100644 modules/nf-core/untar/tests/main.nf.test create mode 100644 modules/nf-core/untar/tests/main.nf.test.snap create mode 100644 modules/nf-core/untar/tests/tags.yml create mode 100644 modules/nf-core/upd/environment.yml create mode 100644 modules/nf-core/vcfanno/environment.yml create mode 100644 modules/nf-core/vcfanno/tests/main.nf.test create mode 100644 modules/nf-core/vcfanno/tests/main.nf.test.snap create mode 100644 modules/nf-core/vcfanno/tests/nextflow.config create mode 100644 modules/nf-core/vcfanno/tests/tags.yml diff --git a/modules/nf-core/bcftools/annotate/environment.yml b/modules/nf-core/bcftools/annotate/environment.yml new file mode 100644 index 00000000..e0abc8d2 --- /dev/null +++ b/modules/nf-core/bcftools/annotate/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_annotate +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/concat/environment.yml b/modules/nf-core/bcftools/concat/environment.yml new file mode 100644 index 00000000..ff0200df --- /dev/null +++ b/modules/nf-core/bcftools/concat/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_concat +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/concat/tests/main.nf.test b/modules/nf-core/bcftools/concat/tests/main.nf.test new file mode 100644 index 00000000..bf1a5f3f --- /dev/null +++ b/modules/nf-core/bcftools/concat/tests/main.nf.test @@ -0,0 +1,108 @@ +nextflow_process { + + name "Test Process BCFTOOLS_CONCAT" + script "../main.nf" + process "BCFTOOLS_CONCAT" + + tag "modules" + tag "modules_nfcore" + tag "bcftools" + tag "bcftools/concat" + + config "./nextflow.config" + + test("sarscov2 - [[vcf1, vcf2], [tbi1, tbi2]]") { + + when { + process { + """ + input[0] = [ + [ id:'test3' ], // meta map + [ + file(params.test_data['homo_sapiens']['illumina']['test_haplotc_cnn_vcf_gz'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_genome_vcf_gz'], checkIfExists: true) + ], + [ + file(params.test_data['homo_sapiens']['illumina']['test_genome_vcf_gz_tbi'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_haplotc_cnn_vcf_gz_tbi'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [[vcf1, vcf2], []]") { + + when { + process { + """ + input[0] = [ + [ id:'test3' ], // meta map + [ + file(params.test_data['homo_sapiens']['illumina']['test_haplotc_cnn_vcf_gz'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_genome_vcf_gz'], checkIfExists: true) + ], + [] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [[vcf1, vcf2], [tbi1, tbi2]] - stub") { + + options "-stub" + when { + process { + """ + input[0] = [ + [ id:'test3' ], // meta map + [ + file(params.test_data['homo_sapiens']['illumina']['test_haplotc_cnn_vcf_gz'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_genome_vcf_gz'], checkIfExists: true) + ], + [ + file(params.test_data['homo_sapiens']['illumina']['test_genome_vcf_gz_tbi'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_haplotc_cnn_vcf_gz_tbi'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.vcf[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/bcftools/concat/tests/main.nf.test.snap b/modules/nf-core/bcftools/concat/tests/main.nf.test.snap new file mode 100644 index 00000000..7344e6e3 --- /dev/null +++ b/modules/nf-core/bcftools/concat/tests/main.nf.test.snap @@ -0,0 +1,43 @@ +{ + "sarscov2 - [[vcf1, vcf2], []]": { + "content": [ + [ + [ + { + "id": "test3" + }, + "test3.vcf.gz:md5,4bcd0afd89f56c5d433f6b6abc44d0a6" + ] + ], + [ + "versions.yml:md5,24ae05eb858733b40fbd3f89743a6d09" + ] + ], + "timestamp": "2023-11-29T13:52:27.03724666" + }, + "sarscov2 - [[vcf1, vcf2], [tbi1, tbi2]]": { + "content": [ + [ + [ + { + "id": "test3" + }, + "test3.vcf.gz:md5,4bcd0afd89f56c5d433f6b6abc44d0a6" + ] + ], + [ + "versions.yml:md5,24ae05eb858733b40fbd3f89743a6d09" + ] + ], + "timestamp": "2023-11-29T13:52:21.468988293" + }, + "sarscov2 - [[vcf1, vcf2], [tbi1, tbi2]] - stub": { + "content": [ + "test3.vcf.gz", + [ + "versions.yml:md5,24ae05eb858733b40fbd3f89743a6d09" + ] + ], + "timestamp": "2023-11-29T13:41:04.716017811" + } +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/concat/tests/nextflow.config b/modules/nf-core/bcftools/concat/tests/nextflow.config new file mode 100644 index 00000000..f3e1e98c --- /dev/null +++ b/modules/nf-core/bcftools/concat/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args = "--no-version" +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/concat/tests/tags.yml b/modules/nf-core/bcftools/concat/tests/tags.yml new file mode 100644 index 00000000..21710d4e --- /dev/null +++ b/modules/nf-core/bcftools/concat/tests/tags.yml @@ -0,0 +1,2 @@ +bcftools/concat: + - "modules/nf-core/bcftools/concat/**" diff --git a/modules/nf-core/bcftools/filter/environment.yml b/modules/nf-core/bcftools/filter/environment.yml new file mode 100644 index 00000000..b2698757 --- /dev/null +++ b/modules/nf-core/bcftools/filter/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_filter +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/merge/environment.yml b/modules/nf-core/bcftools/merge/environment.yml new file mode 100644 index 00000000..55de7cd3 --- /dev/null +++ b/modules/nf-core/bcftools/merge/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_merge +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/norm/environment.yml b/modules/nf-core/bcftools/norm/environment.yml new file mode 100644 index 00000000..fe80e4e7 --- /dev/null +++ b/modules/nf-core/bcftools/norm/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_norm +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/reheader/environment.yml b/modules/nf-core/bcftools/reheader/environment.yml new file mode 100644 index 00000000..aab0dc92 --- /dev/null +++ b/modules/nf-core/bcftools/reheader/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_reheader +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/reheader/tests/bcf.config b/modules/nf-core/bcftools/reheader/tests/bcf.config new file mode 100644 index 00000000..2b7dff55 --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/bcf.config @@ -0,0 +1,4 @@ +process { + ext.args2 = { "--no-version --output-type b" } + ext.prefix = "tested" +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/reheader/tests/main.nf.test b/modules/nf-core/bcftools/reheader/tests/main.nf.test new file mode 100644 index 00000000..f3200cb3 --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/main.nf.test @@ -0,0 +1,197 @@ +nextflow_process { + + name "Test Process BCFTOOLS_REHEADER" + script "../main.nf" + process "BCFTOOLS_REHEADER" + tag "modules" + tag "modules_nfcore" + tag "bcftools" + tag "bcftools/reheader" + + test("sarscov2 - [vcf, [], []], fai - vcf output") { + + config "./vcf.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf, [], []], fai - vcf.gz output") { + + config "./vcf.gz.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf, [], []], fai - bcf output") { + + config "./bcf.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf, header, []], []") { + + config "./vcf.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true), + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + [] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf, [], samples], fai") { + + config "./vcf.config" + when { + + process { + """ + ch_no_samples = Channel.of([ + [ id:'test', single_end:false ], + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + [] + ]) + ch_samples = Channel.of(["samples.txt", "new_name"]) + .collectFile(newLine:true) + input[0] = ch_no_samples.combine(ch_samples) + input[1] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf, [], []], fai - stub") { + + options "-stub" + config "./vcf.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.vcf[0][1]).name, + process.out.versions, + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/bcftools/reheader/tests/main.nf.test.snap b/modules/nf-core/bcftools/reheader/tests/main.nf.test.snap new file mode 100644 index 00000000..112736a1 --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/main.nf.test.snap @@ -0,0 +1,166 @@ +{ + "sarscov2 - [vcf, [], []], fai - vcf output": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "1": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "versions": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ] + } + ], + "timestamp": "2023-11-29T13:05:44.058376693" + }, + "sarscov2 - [vcf, [], []], fai - bcf output": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.bcf.gz:md5,c31d9afd8614832c2a46d9a55682c97a" + ] + ], + "1": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.bcf.gz:md5,c31d9afd8614832c2a46d9a55682c97a" + ] + ], + "versions": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ] + } + ], + "timestamp": "2023-11-29T13:06:03.793372514" + }, + "sarscov2 - [vcf, [], []], fai - vcf.gz output": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf.gz:md5,a1e45fe6d2b386fc2611766e5d2937ee" + ] + ], + "1": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf.gz:md5,a1e45fe6d2b386fc2611766e5d2937ee" + ] + ], + "versions": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ] + } + ], + "timestamp": "2023-11-29T13:05:53.954090441" + }, + "sarscov2 - [vcf, [], []], fai - stub": { + "content": [ + "tested.vcf", + [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ] + ], + "timestamp": "2023-11-29T13:06:33.549685303" + }, + "sarscov2 - [vcf, [], samples], fai": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf:md5,c64c373c10b0be24b29d6f18708ec1e8" + ] + ], + "1": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf:md5,c64c373c10b0be24b29d6f18708ec1e8" + ] + ], + "versions": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ] + } + ], + "timestamp": "2023-11-29T13:06:23.474745156" + }, + "sarscov2 - [vcf, header, []], []": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf:md5,3189bc9a720d5d5d3006bf72d91300cb" + ] + ], + "1": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf:md5,3189bc9a720d5d5d3006bf72d91300cb" + ] + ], + "versions": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ] + } + ], + "timestamp": "2023-11-29T13:06:13.841648691" + } +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/reheader/tests/tags.yml b/modules/nf-core/bcftools/reheader/tests/tags.yml new file mode 100644 index 00000000..c252941e --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/tags.yml @@ -0,0 +1,2 @@ +bcftools/reheader: + - modules/nf-core/bcftools/reheader/** diff --git a/modules/nf-core/bcftools/reheader/tests/vcf.config b/modules/nf-core/bcftools/reheader/tests/vcf.config new file mode 100644 index 00000000..820f2ae6 --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/vcf.config @@ -0,0 +1,4 @@ +process { + ext.args2 = { "--no-version" } + ext.prefix = "tested" +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/reheader/tests/vcf.gz.config b/modules/nf-core/bcftools/reheader/tests/vcf.gz.config new file mode 100644 index 00000000..c3031c31 --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/vcf.gz.config @@ -0,0 +1,4 @@ +process { + ext.args2 = { "--no-version --output-type z" } + ext.prefix = "tested" +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/roh/environment.yml b/modules/nf-core/bcftools/roh/environment.yml new file mode 100644 index 00000000..7a9ee9bd --- /dev/null +++ b/modules/nf-core/bcftools/roh/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_roh +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/sort/environment.yml b/modules/nf-core/bcftools/sort/environment.yml new file mode 100644 index 00000000..89cf911d --- /dev/null +++ b/modules/nf-core/bcftools/sort/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_sort +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/view/environment.yml b/modules/nf-core/bcftools/view/environment.yml new file mode 100644 index 00000000..8937c6da --- /dev/null +++ b/modules/nf-core/bcftools/view/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_view +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/view/tests/main.nf.test b/modules/nf-core/bcftools/view/tests/main.nf.test new file mode 100644 index 00000000..c285674c --- /dev/null +++ b/modules/nf-core/bcftools/view/tests/main.nf.test @@ -0,0 +1,103 @@ +nextflow_process { + + name "Test Process BCFTOOLS_VIEW" + script "../main.nf" + process "BCFTOOLS_VIEW" + + tag "modules" + tag "modules_nfcore" + tag "bcftools" + tag "bcftools/view" + + config "./nextflow.config" + + test("sarscov2 - [vcf, tbi], [], [], []") { + + when { + process { + """ + input[0] = [ + [ id:'out', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) + ] + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [vcf, tbi], vcf, tsv, []") { + + when { + process { + """ + input[0] = [ + [ id:'out', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) + ] + input[1] = file(params.test_data['sarscov2']['illumina']['test3_vcf_gz'], checkIfExists: true) + input[2] = file(params.test_data['sarscov2']['illumina']['test2_vcf_targets_tsv_gz'], checkIfExists: true) + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [vcf, tbi], [], [], [] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'out', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) + ] + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.vcf[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/bcftools/view/tests/main.nf.test.snap b/modules/nf-core/bcftools/view/tests/main.nf.test.snap new file mode 100644 index 00000000..049ac252 --- /dev/null +++ b/modules/nf-core/bcftools/view/tests/main.nf.test.snap @@ -0,0 +1,45 @@ +{ + "sarscov2 - [vcf, tbi], vcf, tsv, []": { + "content": [ + [ + [ + { + "id": "out", + "single_end": false + }, + "out.vcf.gz:md5,3c47ba1a6aa4ef9b3ad800175814d739" + ] + ], + [ + "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" + ] + ], + "timestamp": "2023-11-29T14:27:10.724842996" + }, + "sarscov2 - [vcf, tbi], [], [], [] - stub": { + "content": [ + "out.vcf.gz", + [ + "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" + ] + ], + "timestamp": "2023-11-29T14:27:17.445846794" + }, + "sarscov2 - [vcf, tbi], [], [], []": { + "content": [ + [ + [ + { + "id": "out", + "single_end": false + }, + "out.vcf.gz:md5,a1e45fe6d2b386fc2611766e5d2937ee" + ] + ], + [ + "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" + ] + ], + "timestamp": "2023-11-29T14:27:03.328392594" + } +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/view/tests/nextflow.config b/modules/nf-core/bcftools/view/tests/nextflow.config new file mode 100644 index 00000000..b05aa504 --- /dev/null +++ b/modules/nf-core/bcftools/view/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args = '--no-version' +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/view/tests/tags.yml b/modules/nf-core/bcftools/view/tests/tags.yml new file mode 100644 index 00000000..43b1f0aa --- /dev/null +++ b/modules/nf-core/bcftools/view/tests/tags.yml @@ -0,0 +1,2 @@ +bcftools/view: + - "modules/nf-core/bcftools/view/**" diff --git a/modules/nf-core/bwa/index/environment.yml b/modules/nf-core/bwa/index/environment.yml new file mode 100644 index 00000000..5d3cb323 --- /dev/null +++ b/modules/nf-core/bwa/index/environment.yml @@ -0,0 +1,7 @@ +name: bwa_index +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bwa=0.7.17 diff --git a/modules/nf-core/bwa/index/tests/main.nf.test b/modules/nf-core/bwa/index/tests/main.nf.test new file mode 100644 index 00000000..5fc8d496 --- /dev/null +++ b/modules/nf-core/bwa/index/tests/main.nf.test @@ -0,0 +1,33 @@ +nextflow_process { + + name "Test Process BWA_INDEX" + tag "modules_nfcore" + tag "modules" + tag "bwa" + tag "bwa/index" + script "../main.nf" + process "BWA_INDEX" + + test("BWA index") { + + when { + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/bwa/index/tests/main.nf.test.snap b/modules/nf-core/bwa/index/tests/main.nf.test.snap new file mode 100644 index 00000000..e51ad5bf --- /dev/null +++ b/modules/nf-core/bwa/index/tests/main.nf.test.snap @@ -0,0 +1,43 @@ +{ + "BWA index": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + [ + "genome.amb:md5,3a68b8b2287e07dd3f5f95f4344ba76e", + "genome.ann:md5,c32e11f6c859f166c7525a9c1d583567", + "genome.bwt:md5,0469c30a1e239dd08f68afe66fde99da", + "genome.pac:md5,983e3d2cd6f36e2546e6d25a0da78d66", + "genome.sa:md5,ab3952cabf026b48cd3eb5bccbb636d1" + ] + ] + ], + "1": [ + "versions.yml:md5,0f20525da90e7489a7ebb02adca3265f" + ], + "index": [ + [ + { + "id": "test" + }, + [ + "genome.amb:md5,3a68b8b2287e07dd3f5f95f4344ba76e", + "genome.ann:md5,c32e11f6c859f166c7525a9c1d583567", + "genome.bwt:md5,0469c30a1e239dd08f68afe66fde99da", + "genome.pac:md5,983e3d2cd6f36e2546e6d25a0da78d66", + "genome.sa:md5,ab3952cabf026b48cd3eb5bccbb636d1" + ] + ] + ], + "versions": [ + "versions.yml:md5,0f20525da90e7489a7ebb02adca3265f" + ] + } + ], + "timestamp": "2023-10-17T17:20:20.180927714" + } +} \ No newline at end of file diff --git a/modules/nf-core/bwa/index/tests/tags.yml b/modules/nf-core/bwa/index/tests/tags.yml new file mode 100644 index 00000000..28bb483c --- /dev/null +++ b/modules/nf-core/bwa/index/tests/tags.yml @@ -0,0 +1,2 @@ +bwa/index: + - modules/nf-core/bwa/index/** diff --git a/modules/nf-core/bwamem2/index/environment.yml b/modules/nf-core/bwamem2/index/environment.yml new file mode 100644 index 00000000..26b43917 --- /dev/null +++ b/modules/nf-core/bwamem2/index/environment.yml @@ -0,0 +1,7 @@ +name: bwamem2_index +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bwa-mem2=2.2.1 diff --git a/modules/nf-core/bwamem2/mem/environment.yml b/modules/nf-core/bwamem2/mem/environment.yml new file mode 100644 index 00000000..227d8690 --- /dev/null +++ b/modules/nf-core/bwamem2/mem/environment.yml @@ -0,0 +1,9 @@ +name: bwamem2_mem +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bwa-mem2=2.2.1 + # renovate: datasource=conda depName=bioconda/samtools + - samtools=1.18 diff --git a/modules/nf-core/bwamem2/mem/tests/main.nf.test b/modules/nf-core/bwamem2/mem/tests/main.nf.test new file mode 100644 index 00000000..d7912069 --- /dev/null +++ b/modules/nf-core/bwamem2/mem/tests/main.nf.test @@ -0,0 +1,213 @@ +nextflow_process { + + name "Test Process BWAMEM2_MEM" + script "../main.nf" + process "BWAMEM2_MEM" + + tag "modules" + tag "modules_nfcore" + tag "bwamem2" + tag "bwamem2/mem" + + test("sarscov2 - fastq, index, false") { + + setup { + run("BWAMEM2_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ [:], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BWAMEM2_INDEX.out.index + input[2] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, index, true") { + + setup { + run("BWAMEM2_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ [:], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BWAMEM2_INDEX.out.index + input[2] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, false") { + + setup { + run("BWAMEM2_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ [:], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWAMEM2_INDEX.out.index + input[2] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, true") { + + setup { + run("BWAMEM2_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ [:], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWAMEM2_INDEX.out.index + input[2] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, true - stub") { + + options "-stub" + + setup { + run("BWAMEM2_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ [:], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWAMEM2_INDEX.out.index + input[2] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap b/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap new file mode 100644 index 00000000..db7dd392 --- /dev/null +++ b/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap @@ -0,0 +1,47 @@ +{ + "sarscov2 - [fastq1, fastq2], index, true": { + "content": [ + "test.bam", + [ + "versions.yml:md5,f653bf29c6597481cd0345bd66aafd8a" + ] + ], + "timestamp": "2023-12-04T11:49:07.89544697" + }, + "sarscov2 - [fastq1, fastq2], index, false": { + "content": [ + "test.bam", + [ + "versions.yml:md5,f653bf29c6597481cd0345bd66aafd8a" + ] + ], + "timestamp": "2023-12-04T11:48:56.319355195" + }, + "sarscov2 - [fastq1, fastq2], index, true - stub": { + "content": [ + "test.bam", + [ + "versions.yml:md5,f653bf29c6597481cd0345bd66aafd8a" + ] + ], + "timestamp": "2023-12-04T11:49:17.499101941" + }, + "sarscov2 - fastq, index, false": { + "content": [ + "test.bam", + [ + "versions.yml:md5,f653bf29c6597481cd0345bd66aafd8a" + ] + ], + "timestamp": "2023-12-04T11:48:31.351628851" + }, + "sarscov2 - fastq, index, true": { + "content": [ + "test.bam", + [ + "versions.yml:md5,f653bf29c6597481cd0345bd66aafd8a" + ] + ], + "timestamp": "2023-12-04T11:48:43.978452504" + } +} \ No newline at end of file diff --git a/modules/nf-core/bwamem2/mem/tests/tags.yml b/modules/nf-core/bwamem2/mem/tests/tags.yml new file mode 100644 index 00000000..134efb2b --- /dev/null +++ b/modules/nf-core/bwamem2/mem/tests/tags.yml @@ -0,0 +1,2 @@ +bwamem2/mem: + - "modules/nf-core/bwamem2/mem/**" diff --git a/modules/nf-core/cadd/environment.yml b/modules/nf-core/cadd/environment.yml new file mode 100644 index 00000000..6a21f71e --- /dev/null +++ b/modules/nf-core/cadd/environment.yml @@ -0,0 +1,9 @@ +name: cadd +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::cadd-scripts=1.6 + - anaconda::conda=4.14.0 + - conda-forge::mamba=1.4.0 diff --git a/modules/nf-core/chromograph/environment.yml b/modules/nf-core/chromograph/environment.yml new file mode 100644 index 00000000..4fe69462 --- /dev/null +++ b/modules/nf-core/chromograph/environment.yml @@ -0,0 +1,7 @@ +name: chromograph +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::chromograph=1.3.1 diff --git a/modules/nf-core/cnvnator/cnvnator/environment.yml b/modules/nf-core/cnvnator/cnvnator/environment.yml new file mode 100644 index 00000000..8b7ecaef --- /dev/null +++ b/modules/nf-core/cnvnator/cnvnator/environment.yml @@ -0,0 +1,7 @@ +name: cnvnator_cnvnator +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::cnvnator=0.4.1 diff --git a/modules/nf-core/cnvnator/convert2vcf/environment.yml b/modules/nf-core/cnvnator/convert2vcf/environment.yml new file mode 100644 index 00000000..d324fb04 --- /dev/null +++ b/modules/nf-core/cnvnator/convert2vcf/environment.yml @@ -0,0 +1,7 @@ +name: cnvnator_convert2vcf +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::cnvnator=0.4.1 diff --git a/modules/nf-core/deepvariant/environment.yml b/modules/nf-core/deepvariant/environment.yml new file mode 100644 index 00000000..648a76de --- /dev/null +++ b/modules/nf-core/deepvariant/environment.yml @@ -0,0 +1,5 @@ +name: deepvariant +channels: + - conda-forge + - bioconda + - defaults diff --git a/modules/nf-core/ensemblvep/filtervep/environment.yml b/modules/nf-core/ensemblvep/filtervep/environment.yml new file mode 100644 index 00000000..d84dc89e --- /dev/null +++ b/modules/nf-core/ensemblvep/filtervep/environment.yml @@ -0,0 +1,7 @@ +name: ensemblvep_filtervep +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::ensembl-vep=110.0 diff --git a/modules/nf-core/expansionhunter/environment.yml b/modules/nf-core/expansionhunter/environment.yml new file mode 100644 index 00000000..76edd1ef --- /dev/null +++ b/modules/nf-core/expansionhunter/environment.yml @@ -0,0 +1,7 @@ +name: expansionhunter +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::expansionhunter=5.0.0 diff --git a/modules/nf-core/gatk4/bedtointervallist/environment.yml b/modules/nf-core/gatk4/bedtointervallist/environment.yml new file mode 100644 index 00000000..e7cb4280 --- /dev/null +++ b/modules/nf-core/gatk4/bedtointervallist/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_bedtointervallist +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/collectreadcounts/environment.yml b/modules/nf-core/gatk4/collectreadcounts/environment.yml new file mode 100644 index 00000000..ebc83800 --- /dev/null +++ b/modules/nf-core/gatk4/collectreadcounts/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_collectreadcounts +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/createsequencedictionary/environment.yml b/modules/nf-core/gatk4/createsequencedictionary/environment.yml new file mode 100644 index 00000000..db663e14 --- /dev/null +++ b/modules/nf-core/gatk4/createsequencedictionary/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_createsequencedictionary +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/denoisereadcounts/environment.yml b/modules/nf-core/gatk4/denoisereadcounts/environment.yml new file mode 100644 index 00000000..bcb2ff35 --- /dev/null +++ b/modules/nf-core/gatk4/denoisereadcounts/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_denoisereadcounts +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/determinegermlinecontigploidy/environment.yml b/modules/nf-core/gatk4/determinegermlinecontigploidy/environment.yml new file mode 100644 index 00000000..c30de090 --- /dev/null +++ b/modules/nf-core/gatk4/determinegermlinecontigploidy/environment.yml @@ -0,0 +1,5 @@ +name: gatk4_determinegermlinecontigploidy +channels: + - conda-forge + - bioconda + - defaults diff --git a/modules/nf-core/gatk4/filtermutectcalls/environment.yml b/modules/nf-core/gatk4/filtermutectcalls/environment.yml new file mode 100644 index 00000000..8057d765 --- /dev/null +++ b/modules/nf-core/gatk4/filtermutectcalls/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_filtermutectcalls +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/germlinecnvcaller/environment.yml b/modules/nf-core/gatk4/germlinecnvcaller/environment.yml new file mode 100644 index 00000000..02ce4dd3 --- /dev/null +++ b/modules/nf-core/gatk4/germlinecnvcaller/environment.yml @@ -0,0 +1,5 @@ +name: gatk4_germlinecnvcaller +channels: + - conda-forge + - bioconda + - defaults diff --git a/modules/nf-core/gatk4/intervallisttools/environment.yml b/modules/nf-core/gatk4/intervallisttools/environment.yml new file mode 100644 index 00000000..b70e5e14 --- /dev/null +++ b/modules/nf-core/gatk4/intervallisttools/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_intervallisttools +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/mergebamalignment/environment.yml b/modules/nf-core/gatk4/mergebamalignment/environment.yml new file mode 100644 index 00000000..08a1df23 --- /dev/null +++ b/modules/nf-core/gatk4/mergebamalignment/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_mergebamalignment +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/mergevcfs/environment.yml b/modules/nf-core/gatk4/mergevcfs/environment.yml new file mode 100644 index 00000000..d6c3e51a --- /dev/null +++ b/modules/nf-core/gatk4/mergevcfs/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_mergevcfs +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/mutect2/environment.yml b/modules/nf-core/gatk4/mutect2/environment.yml new file mode 100644 index 00000000..54da66ce --- /dev/null +++ b/modules/nf-core/gatk4/mutect2/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_mutect2 +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/environment.yml b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/environment.yml new file mode 100644 index 00000000..a515d065 --- /dev/null +++ b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/environment.yml @@ -0,0 +1,5 @@ +name: gatk4_postprocessgermlinecnvcalls +channels: + - conda-forge + - bioconda + - defaults diff --git a/modules/nf-core/gatk4/preprocessintervals/environment.yml b/modules/nf-core/gatk4/preprocessintervals/environment.yml new file mode 100644 index 00000000..542d9efd --- /dev/null +++ b/modules/nf-core/gatk4/preprocessintervals/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_preprocessintervals +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/printreads/environment.yml b/modules/nf-core/gatk4/printreads/environment.yml new file mode 100644 index 00000000..7387f5d1 --- /dev/null +++ b/modules/nf-core/gatk4/printreads/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_printreads +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/revertsam/environment.yml b/modules/nf-core/gatk4/revertsam/environment.yml new file mode 100644 index 00000000..0ce7dddc --- /dev/null +++ b/modules/nf-core/gatk4/revertsam/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_revertsam +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/samtofastq/environment.yml b/modules/nf-core/gatk4/samtofastq/environment.yml new file mode 100644 index 00000000..584c3d83 --- /dev/null +++ b/modules/nf-core/gatk4/samtofastq/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_samtofastq +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/selectvariants/environment.yml b/modules/nf-core/gatk4/selectvariants/environment.yml new file mode 100644 index 00000000..404bafde --- /dev/null +++ b/modules/nf-core/gatk4/selectvariants/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_selectvariants +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/shiftfasta/environment.yml b/modules/nf-core/gatk4/shiftfasta/environment.yml new file mode 100644 index 00000000..381a22c0 --- /dev/null +++ b/modules/nf-core/gatk4/shiftfasta/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_shiftfasta +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/splitintervals/environment.yml b/modules/nf-core/gatk4/splitintervals/environment.yml new file mode 100644 index 00000000..6b347417 --- /dev/null +++ b/modules/nf-core/gatk4/splitintervals/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_splitintervals +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/gatk4/variantfiltration/environment.yml b/modules/nf-core/gatk4/variantfiltration/environment.yml new file mode 100644 index 00000000..cc16bc0e --- /dev/null +++ b/modules/nf-core/gatk4/variantfiltration/environment.yml @@ -0,0 +1,7 @@ +name: gatk4_variantfiltration +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gatk4=4.4.0.0 diff --git a/modules/nf-core/genmod/annotate/environment.yml b/modules/nf-core/genmod/annotate/environment.yml new file mode 100644 index 00000000..6ca55014 --- /dev/null +++ b/modules/nf-core/genmod/annotate/environment.yml @@ -0,0 +1,7 @@ +name: genmod_annotate +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::genmod=3.7.4 diff --git a/modules/nf-core/genmod/compound/environment.yml b/modules/nf-core/genmod/compound/environment.yml new file mode 100644 index 00000000..565143f9 --- /dev/null +++ b/modules/nf-core/genmod/compound/environment.yml @@ -0,0 +1,7 @@ +name: genmod_compound +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::genmod=3.7.4 diff --git a/modules/nf-core/genmod/models/environment.yml b/modules/nf-core/genmod/models/environment.yml new file mode 100644 index 00000000..ff0de822 --- /dev/null +++ b/modules/nf-core/genmod/models/environment.yml @@ -0,0 +1,8 @@ +name: genmod_models +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::genmod=3.7.4 + - conda-forge::python=3.4.5 diff --git a/modules/nf-core/genmod/score/environment.yml b/modules/nf-core/genmod/score/environment.yml new file mode 100644 index 00000000..a09ee359 --- /dev/null +++ b/modules/nf-core/genmod/score/environment.yml @@ -0,0 +1,7 @@ +name: genmod_score +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::genmod=3.7.4 diff --git a/modules/nf-core/glnexus/environment.yml b/modules/nf-core/glnexus/environment.yml new file mode 100644 index 00000000..922214e8 --- /dev/null +++ b/modules/nf-core/glnexus/environment.yml @@ -0,0 +1,7 @@ +name: glnexus +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::glnexus=1.4.1 diff --git a/modules/nf-core/haplocheck/environment.yml b/modules/nf-core/haplocheck/environment.yml new file mode 100644 index 00000000..8d06a67e --- /dev/null +++ b/modules/nf-core/haplocheck/environment.yml @@ -0,0 +1,7 @@ +name: haplocheck +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::haplocheck=1.3.3 diff --git a/modules/nf-core/haplogrep2/classify/environment.yml b/modules/nf-core/haplogrep2/classify/environment.yml new file mode 100644 index 00000000..12949452 --- /dev/null +++ b/modules/nf-core/haplogrep2/classify/environment.yml @@ -0,0 +1,7 @@ +name: haplogrep2_classify +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::haplogrep=2.4.0 diff --git a/modules/nf-core/hmtnote/annotate/environment.yml b/modules/nf-core/hmtnote/annotate/environment.yml new file mode 100644 index 00000000..46bd87aa --- /dev/null +++ b/modules/nf-core/hmtnote/annotate/environment.yml @@ -0,0 +1,7 @@ +name: hmtnote_annotate +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::hmtnote=0.7.2 diff --git a/modules/nf-core/manta/germline/environment.yml b/modules/nf-core/manta/germline/environment.yml new file mode 100644 index 00000000..4a63d308 --- /dev/null +++ b/modules/nf-core/manta/germline/environment.yml @@ -0,0 +1,7 @@ +name: manta_germline +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::manta=1.6.0 diff --git a/modules/nf-core/mosdepth/environment.yml b/modules/nf-core/mosdepth/environment.yml new file mode 100644 index 00000000..88c7126c --- /dev/null +++ b/modules/nf-core/mosdepth/environment.yml @@ -0,0 +1,8 @@ +name: mosdepth +channels: + - conda-forge + - bioconda + - defaults +dependencies: + # renovate: datasource=conda depName=bioconda/mosdepth + - mosdepth=0.3.6 diff --git a/modules/nf-core/mosdepth/tests/main.nf.test b/modules/nf-core/mosdepth/tests/main.nf.test new file mode 100644 index 00000000..d991f819 --- /dev/null +++ b/modules/nf-core/mosdepth/tests/main.nf.test @@ -0,0 +1,260 @@ +nextflow_process { + + name "Test Process MOSDEPTH" + script "../main.nf" + process "MOSDEPTH" + + tag "modules" + tag "modules_nfcore" + tag "mosdepth" + + test("homo_sapiens - bam, bai, []") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true), + [] + ] + input[1] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("homo_sapiens - bam, bai, bed") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true), + file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) + ] + input[1] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("homo_sapiens - cram, crai, []") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram_crai'], checkIfExists: true), + [] + ] + input[1] = [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("homo_sapiens - cram, crai, bed") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram_crai'], checkIfExists: true), + file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) + ] + input[1] = [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("homo_sapiens - bam, bai, [] - window") { + + config "./window.config" + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true), + [] + ] + input[1] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("homo_sapiens - bam, bai, [] - quantized") { + + config "./quantized.config" + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true), + [] + ] + input[1] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("homo_sapiens - bam, bai, bed - thresholds") { + + config "./threshold.config" + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true), + file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) + ] + input[1] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("homo_sapiens - bam, bai, bed - fail") { + + config "./window.config" + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true), + file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) + ] + input[1] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.failed } + ) + } + + } + + test("homo_sapiens - bam, bai, [] - stub") { + + options "-stub" + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true), + file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) + ] + input[1] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.global_txt[0][1]).name, + file(process.out.summary_txt[0][1]).name, + file(process.out.regions_txt[0][1]).name, + file(process.out.per_base_d4[0][1]).name, + file(process.out.per_base_bed[0][1]).name, + file(process.out.per_base_csi[0][1]).name, + file(process.out.regions_bed[0][1]).name, + file(process.out.regions_csi[0][1]).name, + file(process.out.quantized_bed[0][1]).name, + file(process.out.quantized_csi[0][1]).name, + file(process.out.thresholds_bed[0][1]).name, + file(process.out.thresholds_csi[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/mosdepth/tests/main.nf.test.snap b/modules/nf-core/mosdepth/tests/main.nf.test.snap new file mode 100644 index 00000000..dc4d9508 --- /dev/null +++ b/modules/nf-core/mosdepth/tests/main.nf.test.snap @@ -0,0 +1,1145 @@ +{ + "homo_sapiens - bam, bai, [] - stub": { 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true + }, + "test.per-base.bed.gz.csi:md5,9e649ac749ff6c6073bef5ab63e8aaa4" + ] + ], + "per_base_d4": [ + + ], + "quantized_bed": [ + + ], + "quantized_csi": [ + + ], + "regions_bed": [ + [ + { + "id": "test", + "single_end": true + }, + "test.regions.bed.gz:md5,5d398caf7171ec4406278e2add3009ae" + ] + ], + "regions_csi": [ + [ + { + "id": "test", + "single_end": true + }, + "test.regions.bed.gz.csi:md5,47669cfe41f3e222e74d81e1b1be191f" + ] + ], + "regions_txt": [ + [ + { + "id": "test", + "single_end": true + }, + "test.mosdepth.region.dist.txt:md5,e82e90c7d508a135b5a8a7cd6933452e" + ] + ], + "summary_txt": [ + [ + { + "id": "test", + "single_end": true + }, + "test.mosdepth.summary.txt:md5,96c037f769974b904beb53edc4f56d82" + ] + ], + "thresholds_bed": [ + + ], + "thresholds_csi": [ + + ], + "versions": [ + "versions.yml:md5,f8b1896c9c6784181f1234e87225f0e8" + ] + } + ], + "timestamp": "2023-11-27T14:47:18.171150781" + }, + "homo_sapiens - bam, bai, [] - quantized": { + "content": [ + 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index 00000000..5cd2e08e --- /dev/null +++ b/modules/nf-core/mosdepth/tests/tags.yml @@ -0,0 +1,2 @@ +mosdepth: + - "modules/nf-core/mosdepth/**" diff --git a/modules/nf-core/mosdepth/tests/threshold.config b/modules/nf-core/mosdepth/tests/threshold.config new file mode 100644 index 00000000..9b014ddf --- /dev/null +++ b/modules/nf-core/mosdepth/tests/threshold.config @@ -0,0 +1,3 @@ +process { + ext.args = "--thresholds 1,10,20,30" +} \ No newline at end of file diff --git a/modules/nf-core/mosdepth/tests/window.config b/modules/nf-core/mosdepth/tests/window.config new file mode 100644 index 00000000..7a0f755c --- /dev/null +++ b/modules/nf-core/mosdepth/tests/window.config @@ -0,0 +1,3 @@ +process { + ext.args = "--by 100" +} \ No newline at end of file diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap new file mode 100644 index 00000000..d37e7304 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -0,0 +1,21 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:02:49.911994" + }, + "sarscov2 single-end [fastqc] - stub": { + "content": [ + [ + "multiqc_report.html", + "multiqc_data", + "multiqc_plots", + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:03:14.524346" + } +} \ No newline at end of file diff --git a/modules/nf-core/peddy/environment.yml b/modules/nf-core/peddy/environment.yml new file mode 100644 index 00000000..00e7cebe --- /dev/null +++ b/modules/nf-core/peddy/environment.yml @@ -0,0 +1,7 @@ +name: peddy +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::peddy=0.4.8 diff --git a/modules/nf-core/picard/addorreplacereadgroups/environment.yml b/modules/nf-core/picard/addorreplacereadgroups/environment.yml new file mode 100644 index 00000000..d7d2c6c0 --- /dev/null +++ b/modules/nf-core/picard/addorreplacereadgroups/environment.yml @@ -0,0 +1,7 @@ +name: picard_addorreplacereadgroups +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::picard=3.1.1 diff --git a/modules/nf-core/picard/collecthsmetrics/environment.yml b/modules/nf-core/picard/collecthsmetrics/environment.yml new file mode 100644 index 00000000..fbeab71b --- /dev/null +++ b/modules/nf-core/picard/collecthsmetrics/environment.yml @@ -0,0 +1,7 @@ +name: picard_collecthsmetrics +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::picard=3.1.1 diff --git a/modules/nf-core/picard/collectmultiplemetrics/environment.yml b/modules/nf-core/picard/collectmultiplemetrics/environment.yml new file mode 100644 index 00000000..79b33280 --- /dev/null +++ b/modules/nf-core/picard/collectmultiplemetrics/environment.yml @@ -0,0 +1,7 @@ +name: picard_collectmultiplemetrics +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::picard=3.1.1 diff --git a/modules/nf-core/picard/collectwgsmetrics/environment.yml b/modules/nf-core/picard/collectwgsmetrics/environment.yml new file mode 100644 index 00000000..6a0b9258 --- /dev/null +++ b/modules/nf-core/picard/collectwgsmetrics/environment.yml @@ -0,0 +1,8 @@ +name: picard_collectwgsmetrics +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::picard=3.1.1 + - r::r-base diff --git a/modules/nf-core/picard/liftovervcf/environment.yml b/modules/nf-core/picard/liftovervcf/environment.yml new file mode 100644 index 00000000..e953ec95 --- /dev/null +++ b/modules/nf-core/picard/liftovervcf/environment.yml @@ -0,0 +1,7 @@ +name: picard_liftovervcf +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::picard=3.1.1 diff --git a/modules/nf-core/picard/markduplicates/environment.yml b/modules/nf-core/picard/markduplicates/environment.yml new file mode 100644 index 00000000..58b795f5 --- /dev/null +++ b/modules/nf-core/picard/markduplicates/environment.yml @@ -0,0 +1,7 @@ +name: picard_markduplicates +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::picard=3.1.1 diff --git a/modules/nf-core/picard/markduplicates/tests/main.nf.test b/modules/nf-core/picard/markduplicates/tests/main.nf.test new file mode 100644 index 00000000..b2bba094 --- /dev/null +++ b/modules/nf-core/picard/markduplicates/tests/main.nf.test @@ -0,0 +1,111 @@ +nextflow_process { + + name "Test Process PICARD_MARKDUPLICATES" + script "../main.nf" + process "PICARD_MARKDUPLICATES" + config "./nextflow.config" + tag "modules" + tag "modules_nfcore" + tag "picard" + tag "picard/markduplicates" + + test("sarscov2 - bam, fasta, fai - sorted bam") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = [ + [ id:'genome' ], + file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + path(process.out.metrics.get(0).get(1)).readLines()[0..2], + process.out.versions + ).match() } + ) + } + } + + test("sarscov2 - bam, fasta, fai - unsorted bam") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = [ + [ id:'genome' ], + file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + path(process.out.metrics.get(0).get(1)).readLines()[0..2], + process.out.versions + ).match() } + ) + } + } + + test("homo_sapiens - cram, fasta, fai") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = [ + [ id:'genome' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + path(process.out.metrics.get(0).get(1)).readLines()[0..2], + process.out.versions + ).match() } + ) + } + } + +} diff --git a/modules/nf-core/picard/markduplicates/tests/main.nf.test.snap b/modules/nf-core/picard/markduplicates/tests/main.nf.test.snap new file mode 100644 index 00000000..cd788a4d --- /dev/null +++ b/modules/nf-core/picard/markduplicates/tests/main.nf.test.snap @@ -0,0 +1,44 @@ +{ + "sarscov2 - bam, fasta, fai - unsorted bam": { + "content": [ + "test.marked.bam", + [ + "## htsjdk.samtools.metrics.StringHeader", + "# MarkDuplicates --INPUT test.paired_end.bam --OUTPUT test.marked.bam --METRICS_FILE test.marked.MarkDuplicates.metrics.txt --ASSUME_SORT_ORDER queryname --REFERENCE_SEQUENCE genome.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --REMOVE_DUPLICATES false --ASSUME_SORTED false --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --VALIDATION_STRINGENCY STRICT --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false", + "## htsjdk.samtools.metrics.StringHeader" + ], + [ + "versions.yml:md5,b699af51b1956f3810f8a7c066e0ab17" + ] + ], + "timestamp": "2023-11-28T10:50:37.735339781" + }, + "homo_sapiens - cram, fasta, fai": { + "content": [ + "test.marked.bam", + [ + "## htsjdk.samtools.metrics.StringHeader", + "# MarkDuplicates --INPUT test.paired_end.sorted.cram --OUTPUT test.marked.bam --METRICS_FILE test.marked.MarkDuplicates.metrics.txt --ASSUME_SORT_ORDER queryname --REFERENCE_SEQUENCE genome.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --REMOVE_DUPLICATES false --ASSUME_SORTED false --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --VALIDATION_STRINGENCY STRICT --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false", + "## htsjdk.samtools.metrics.StringHeader" + ], + [ + "versions.yml:md5,b699af51b1956f3810f8a7c066e0ab17" + ] + ], + "timestamp": "2023-11-28T10:50:48.897954543" + }, + "sarscov2 - bam, fasta, fai - sorted bam": { + "content": [ + "test.marked.bam", + [ + "## htsjdk.samtools.metrics.StringHeader", + "# MarkDuplicates --INPUT test.paired_end.sorted.bam --OUTPUT test.marked.bam --METRICS_FILE test.marked.MarkDuplicates.metrics.txt --ASSUME_SORT_ORDER queryname --REFERENCE_SEQUENCE genome.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --REMOVE_DUPLICATES false --ASSUME_SORTED false --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --VALIDATION_STRINGENCY STRICT --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false", + "## htsjdk.samtools.metrics.StringHeader" + ], + [ + "versions.yml:md5,b699af51b1956f3810f8a7c066e0ab17" + ] + ], + "timestamp": "2023-11-28T10:50:26.591387512" + } +} \ No newline at end of file diff --git a/modules/nf-core/picard/markduplicates/tests/nextflow.config b/modules/nf-core/picard/markduplicates/tests/nextflow.config new file mode 100644 index 00000000..02818dd6 --- /dev/null +++ b/modules/nf-core/picard/markduplicates/tests/nextflow.config @@ -0,0 +1,6 @@ +process { + withName: PICARD_MARKDUPLICATES { + ext.prefix = { "${meta.id}.marked" } + ext.args = '--ASSUME_SORT_ORDER queryname' + } +} diff --git a/modules/nf-core/picard/markduplicates/tests/tags.yml b/modules/nf-core/picard/markduplicates/tests/tags.yml new file mode 100644 index 00000000..4f213d62 --- /dev/null +++ b/modules/nf-core/picard/markduplicates/tests/tags.yml @@ -0,0 +1,2 @@ +picard/markduplicates: + - modules/nf-core/picard/markduplicates/** diff --git a/modules/nf-core/picard/renamesampleinvcf/environment.yml b/modules/nf-core/picard/renamesampleinvcf/environment.yml new file mode 100644 index 00000000..3e7d8eb5 --- /dev/null +++ b/modules/nf-core/picard/renamesampleinvcf/environment.yml @@ -0,0 +1,7 @@ +name: picard_renamesampleinvcf +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::picard=3.1.1 diff --git a/modules/nf-core/picard/sortvcf/environment.yml b/modules/nf-core/picard/sortvcf/environment.yml new file mode 100644 index 00000000..f1dbb6b9 --- /dev/null +++ b/modules/nf-core/picard/sortvcf/environment.yml @@ -0,0 +1,7 @@ +name: picard_sortvcf +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::picard=3.1.1 diff --git a/modules/nf-core/qualimap/bamqc/environment.yml b/modules/nf-core/qualimap/bamqc/environment.yml new file mode 100644 index 00000000..3f30d0cd --- /dev/null +++ b/modules/nf-core/qualimap/bamqc/environment.yml @@ -0,0 +1,7 @@ +name: qualimap_bamqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::qualimap=2.3 diff --git a/modules/nf-core/qualimap/bamqc/tests/main.nf.test b/modules/nf-core/qualimap/bamqc/tests/main.nf.test new file mode 100644 index 00000000..5447fe68 --- /dev/null +++ b/modules/nf-core/qualimap/bamqc/tests/main.nf.test @@ -0,0 +1,39 @@ +nextflow_process { + + name "Test Process QUALIMAP_BAMQC" + script "../main.nf" + process "QUALIMAP_BAMQC" + tag "modules" + tag "modules_nfcore" + tag "qualimap" + tag "qualimap/bamqc" + + test("homo_sapiens [BAM]") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) + ] + input[1] = [] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert path("${process.out.results[0][1]}/qualimapReport.html").exists() }, + { assert snapshot( + path("${process.out.results[0][1]}/genome_results.txt"), + process.out.versions) + .match() } + ) + } + } +} \ No newline at end of file diff --git a/modules/nf-core/qualimap/bamqc/tests/main.nf.test.snap b/modules/nf-core/qualimap/bamqc/tests/main.nf.test.snap new file mode 100644 index 00000000..782f7d23 --- /dev/null +++ b/modules/nf-core/qualimap/bamqc/tests/main.nf.test.snap @@ -0,0 +1,11 @@ +{ + "homo_sapiens [BAM]": { + "content": [ + "genome_results.txt:md5,45103d63ba82df2b905eb04819c32dd3", + [ + "versions.yml:md5,9024d7d0a189d8be1485249ae591b907" + ] + ], + "timestamp": "2024-01-11T14:36:07.836508" + } +} \ No newline at end of file diff --git a/modules/nf-core/qualimap/bamqc/tests/tags.yml b/modules/nf-core/qualimap/bamqc/tests/tags.yml new file mode 100644 index 00000000..b2b5eb6f --- /dev/null +++ b/modules/nf-core/qualimap/bamqc/tests/tags.yml @@ -0,0 +1,2 @@ +qualimap/bamqc: + - modules/nf-core/qualimap/bamqc/** diff --git a/modules/nf-core/rhocall/annotate/environment.yml b/modules/nf-core/rhocall/annotate/environment.yml new file mode 100644 index 00000000..5a00600f --- /dev/null +++ b/modules/nf-core/rhocall/annotate/environment.yml @@ -0,0 +1,7 @@ +name: rhocall_annotate +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::rhocall=0.5.1 diff --git a/modules/nf-core/samtools/faidx/environment.yml b/modules/nf-core/samtools/faidx/environment.yml new file mode 100644 index 00000000..01ccbcc7 --- /dev/null +++ b/modules/nf-core/samtools/faidx/environment.yml @@ -0,0 +1,7 @@ +name: samtools_faidx +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.18 diff --git a/modules/nf-core/samtools/index/environment.yml b/modules/nf-core/samtools/index/environment.yml new file mode 100644 index 00000000..296ed99e --- /dev/null +++ b/modules/nf-core/samtools/index/environment.yml @@ -0,0 +1,7 @@ +name: samtools_index +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.18 diff --git a/modules/nf-core/samtools/index/tests/csi.nextflow.config b/modules/nf-core/samtools/index/tests/csi.nextflow.config new file mode 100644 index 00000000..0ed260ef --- /dev/null +++ b/modules/nf-core/samtools/index/tests/csi.nextflow.config @@ -0,0 +1,7 @@ +process { + + withName: SAMTOOLS_INDEX { + ext.args = '-c' + } + +} diff --git a/modules/nf-core/samtools/index/tests/main.nf.test b/modules/nf-core/samtools/index/tests/main.nf.test new file mode 100644 index 00000000..c76a9169 --- /dev/null +++ b/modules/nf-core/samtools/index/tests/main.nf.test @@ -0,0 +1,87 @@ +nextflow_process { + + name "Test Process SAMTOOLS_INDEX" + script "../main.nf" + process "SAMTOOLS_INDEX" + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/index" + + test("sarscov2 [BAI]") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.bai).match("bai") }, + { assert path(process.out.versions.get(0)).getText().contains("samtools") } + ) + } + } + + test("homo_sapiens [CRAI]") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.crai).match("crai") }, + { assert path(process.out.versions.get(0)).getText().contains("samtools") } + ) + } + } + + test("homo_sapiens [CSI]") { + + config "./csi.nextflow.config" + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert path(process.out.csi.get(0).get(1)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("samtools") } + ) + } + } +} diff --git a/modules/nf-core/samtools/index/tests/main.nf.test.snap b/modules/nf-core/samtools/index/tests/main.nf.test.snap new file mode 100644 index 00000000..b3baee7f --- /dev/null +++ b/modules/nf-core/samtools/index/tests/main.nf.test.snap @@ -0,0 +1,28 @@ +{ + "crai": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.paired_end.recalibrated.sorted.cram.crai:md5,14bc3bd5c89cacc8f4541f9062429029" + ] + ] + ], + "timestamp": "2023-11-15T15:17:37.30801" + }, + "bai": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.paired_end.sorted.bam.bai:md5,704c10dd1326482448ca3073fdebc2f4" + ] + ] + ], + "timestamp": "2023-11-15T15:17:30.869234" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/index/tests/tags.yml b/modules/nf-core/samtools/index/tests/tags.yml new file mode 100644 index 00000000..e0f58a7a --- /dev/null +++ b/modules/nf-core/samtools/index/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/index: + - modules/nf-core/samtools/index/** diff --git a/modules/nf-core/samtools/merge/environment.yml b/modules/nf-core/samtools/merge/environment.yml new file mode 100644 index 00000000..1f7fe49f --- /dev/null +++ b/modules/nf-core/samtools/merge/environment.yml @@ -0,0 +1,7 @@ +name: samtools_merge +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.18 diff --git a/modules/nf-core/samtools/merge/tests/index.config b/modules/nf-core/samtools/merge/tests/index.config new file mode 100644 index 00000000..8c5668cf --- /dev/null +++ b/modules/nf-core/samtools/merge/tests/index.config @@ -0,0 +1,3 @@ +process { + ext.args = "--write-index" +} \ No newline at end of file diff --git a/modules/nf-core/samtools/merge/tests/main.nf.test b/modules/nf-core/samtools/merge/tests/main.nf.test new file mode 100644 index 00000000..024f9f70 --- /dev/null +++ b/modules/nf-core/samtools/merge/tests/main.nf.test @@ -0,0 +1,156 @@ +nextflow_process { + + name "Test Process SAMTOOLS_MERGE" + script "../main.nf" + process "SAMTOOLS_MERGE" + + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/merge" + + test("sarscov2 - [bam1, bam2, bam3], [], []") { + + config "./index.config" + + when { + process { + """ + input[0] = [ + [ id: 'test' ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_paired_end_methylated_sorted_bam'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_single_end_sorted_bam'], checkIfExists: true) + ] + ] + input[1] = [[],[]] + input[2] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.cram, + file(process.out.csi[0][1]).name, + process.out.crai, + process.out.versions + ).match() } + ) + } + + } + + test("homo_sapiens - [cram1, cram2], fasta, fai") { + + config "./index.config" + + when { + process { + """ + input[0] = [ + [ id: 'test' ], // meta map + [ + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test2_paired_end_recalibrated_sorted_cram'], checkIfExists: true), + ] + ] + input[1] = [ + [id:'genome'], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = [ + [id:'genome'], + file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.cram[0][1]).name, + process.out.bam, + file(process.out.crai[0][1]).name, + process.out.csi, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - bam, [], []") { + + when { + process { + """ + input[0] = [ + [ id: 'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_methylated_sorted_bam'], checkIfExists: true), + ] + input[1] = [[],[]] + input[2] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.cram, + process.out.crai, + process.out.csi, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [bam1, bam2, bam3], [], [] - stub") { + + config "./index.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id: 'test' ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_paired_end_methylated_sorted_bam'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_single_end_sorted_bam'], checkIfExists: true) + ] + ] + input[1] = [[],[]] + input[2] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.cram, + file(process.out.csi[0][1]).name, + process.out.crai, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/samtools/merge/tests/main.nf.test.snap b/modules/nf-core/samtools/merge/tests/main.nf.test.snap new file mode 100644 index 00000000..3ab57d81 --- /dev/null +++ b/modules/nf-core/samtools/merge/tests/main.nf.test.snap @@ -0,0 +1,68 @@ +{ + "sarscov2 - bam, [], []": { + "content": [ + "test.bam", + [ + + ], + [ + + ], + [ + + ], + [ + "versions.yml:md5,71986103374bdddb2e3093d20e7d06cb" + ] + ], + "timestamp": "2023-12-04T17:13:30.244841621" + }, + "sarscov2 - [bam1, bam2, bam3], [], [] - stub": { + "content": [ + "test.bam", + [ + + ], + "test.csi", + [ + + ], + [ + "versions.yml:md5,71986103374bdddb2e3093d20e7d06cb" + ] + ], + "timestamp": "2023-12-04T17:10:14.861445721" + }, + "homo_sapiens - [cram1, cram2], fasta, fai": { + "content": [ + "test.cram", + [ + + ], + "test.cram.crai", + [ + + ], + [ + "versions.yml:md5,71986103374bdddb2e3093d20e7d06cb" + ] + ], + "timestamp": "2023-12-04T17:09:29.716002618" + }, + "sarscov2 - [bam1, bam2, bam3], [], []": { + "content": [ + "test.bam", + [ + + ], + "test.bam.csi", + [ + + ], + [ + "versions.yml:md5,71986103374bdddb2e3093d20e7d06cb" + ] + ], + "timestamp": "2023-12-04T17:08:42.329973045" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/merge/tests/tags.yml b/modules/nf-core/samtools/merge/tests/tags.yml new file mode 100644 index 00000000..b869abcb --- /dev/null +++ b/modules/nf-core/samtools/merge/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/merge: + - "modules/nf-core/samtools/merge/**" diff --git a/modules/nf-core/samtools/sort/environment.yml b/modules/nf-core/samtools/sort/environment.yml new file mode 100644 index 00000000..cd50868c --- /dev/null +++ b/modules/nf-core/samtools/sort/environment.yml @@ -0,0 +1,7 @@ +name: samtools_sort +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.18 diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test b/modules/nf-core/samtools/sort/tests/main.nf.test new file mode 100644 index 00000000..abb80978 --- /dev/null +++ b/modules/nf-core/samtools/sort/tests/main.nf.test @@ -0,0 +1,73 @@ +nextflow_process { + + name "Test Process SAMTOOLS_SORT" + script "../main.nf" + process "SAMTOOLS_SORT" + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/sort" + + test("test_samtools_sort") { + + config "./nextflow.config" + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + [ + file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("test_samtools_sort_stub") { + + config "./nextflow.config" + options "-stub-run" + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + [ + file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test.snap b/modules/nf-core/samtools/sort/tests/main.nf.test.snap new file mode 100644 index 00000000..ff722259 --- /dev/null +++ b/modules/nf-core/samtools/sort/tests/main.nf.test.snap @@ -0,0 +1,48 @@ +{ + "test_samtools_sort": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.sorted.bam:md5,ea6a0fef94eb534e901f107a05a33a06" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80" + ], + "bam": [ + [ + { + "id": "test", + "single_end": false + }, + "test.sorted.bam:md5,ea6a0fef94eb534e901f107a05a33a06" + ] + ], + "csi": [ + + ], + "versions": [ + "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80" + ] + } + ], + "timestamp": "2023-12-04T11:11:22.005628301" + }, + "test_samtools_sort_stub": { + "content": [ + "test.sorted.bam", + [ + "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80" + ] + ], + "timestamp": "2023-12-04T17:47:22.314445935" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/sort/tests/nextflow.config b/modules/nf-core/samtools/sort/tests/nextflow.config new file mode 100644 index 00000000..d0f35086 --- /dev/null +++ b/modules/nf-core/samtools/sort/tests/nextflow.config @@ -0,0 +1,7 @@ +process { + + withName: SAMTOOLS_SORT { + ext.prefix = { "${meta.id}.sorted" } + } + +} diff --git a/modules/nf-core/samtools/sort/tests/tags.yml b/modules/nf-core/samtools/sort/tests/tags.yml new file mode 100644 index 00000000..cd63ea20 --- /dev/null +++ b/modules/nf-core/samtools/sort/tests/tags.yml @@ -0,0 +1,3 @@ +samtools/sort: + - modules/nf-core/samtools/sort/** + - tests/modules/nf-core/samtools/sort/** diff --git a/modules/nf-core/samtools/stats/environment.yml b/modules/nf-core/samtools/stats/environment.yml new file mode 100644 index 00000000..b89ce647 --- /dev/null +++ b/modules/nf-core/samtools/stats/environment.yml @@ -0,0 +1,7 @@ +name: samtools_stats +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.18 diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test b/modules/nf-core/samtools/stats/tests/main.nf.test new file mode 100644 index 00000000..20c3efe1 --- /dev/null +++ b/modules/nf-core/samtools/stats/tests/main.nf.test @@ -0,0 +1,78 @@ +nextflow_process { + + name "Test Process SAMTOOLS_STATS" + script "../main.nf" + process "SAMTOOLS_STATS" + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/stats" + + test("SAMTOOLS STATS Should run without failures") { + + when { + params { + + outdir = "$outputDir" + } + process { + """ + // define inputs of the process here. + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true) + + ] + input[1] = [[],[]] + """ + + } + } + + then { + assertAll( + {assert process.success}, + {assert snapshot(process.out).match()} + ) + } + + } + + test("SAMTOOLS CRAM Should run without failures") { + + when { + params { + + outdir = "$outputDir" + } + process { + """ + // define inputs of the process here + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram_crai'], checkIfExists: true) + + ] + input[1] = [ + [ id:'genome' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + + + } + + then { + assertAll( + {assert process.success}, + {assert snapshot(process.out).match()} + ) + } + + } + + +} diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test.snap b/modules/nf-core/samtools/stats/tests/main.nf.test.snap new file mode 100644 index 00000000..025c83a5 --- /dev/null +++ b/modules/nf-core/samtools/stats/tests/main.nf.test.snap @@ -0,0 +1,64 @@ +{ + "SAMTOOLS STATS Should run without failures": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,045a48208b1c6f5b8af4347fe31f4def" + ] + ], + "1": [ + "versions.yml:md5,650a365c6635001436008350ae83337c" + ], + "stats": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,045a48208b1c6f5b8af4347fe31f4def" + ] + ], + "versions": [ + "versions.yml:md5,650a365c6635001436008350ae83337c" + ] + } + ], + "timestamp": "2023-12-04T11:07:28.26821485" + }, + "SAMTOOLS CRAM Should run without failures": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,dfbfa130d4a6925ddd1931dcd8354a43" + ] + ], + "1": [ + "versions.yml:md5,650a365c6635001436008350ae83337c" + ], + "stats": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,dfbfa130d4a6925ddd1931dcd8354a43" + ] + ], + "versions": [ + "versions.yml:md5,650a365c6635001436008350ae83337c" + ] + } + ], + "timestamp": "2023-12-04T11:07:50.356233402" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/stats/tests/tags.yml b/modules/nf-core/samtools/stats/tests/tags.yml new file mode 100644 index 00000000..7c28e30f --- /dev/null +++ b/modules/nf-core/samtools/stats/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/stats: + - modules/nf-core/samtools/stats/** diff --git a/modules/nf-core/samtools/view/environment.yml b/modules/nf-core/samtools/view/environment.yml new file mode 100644 index 00000000..99aa69d0 --- /dev/null +++ b/modules/nf-core/samtools/view/environment.yml @@ -0,0 +1,7 @@ +name: samtools_view +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.18 diff --git a/modules/nf-core/samtools/view/tests/bam.config b/modules/nf-core/samtools/view/tests/bam.config new file mode 100644 index 00000000..c10d1081 --- /dev/null +++ b/modules/nf-core/samtools/view/tests/bam.config @@ -0,0 +1,3 @@ +process { + ext.args = "--output-fmt bam" +} \ No newline at end of file diff --git a/modules/nf-core/samtools/view/tests/bam_index.config b/modules/nf-core/samtools/view/tests/bam_index.config new file mode 100644 index 00000000..771ae033 --- /dev/null +++ b/modules/nf-core/samtools/view/tests/bam_index.config @@ -0,0 +1,3 @@ +process { + ext.args = "--output-fmt bam --write-index" +} \ No newline at end of file diff --git a/modules/nf-core/samtools/view/tests/main.nf.test b/modules/nf-core/samtools/view/tests/main.nf.test new file mode 100644 index 00000000..89ed3555 --- /dev/null +++ b/modules/nf-core/samtools/view/tests/main.nf.test @@ -0,0 +1,231 @@ +nextflow_process { + + name "Test Process SAMTOOLS_VIEW" + script "../main.nf" + process "SAMTOOLS_VIEW" + + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/view" + + test("sarscov2 - [bam, []], [], []") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true), + [] + ] + input[1] = [[],[]] + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.cram, + process.out.sam, + process.out.bai, + process.out.crai, + process.out.csi, + process.out.versions + ).match() } + ) + } + + } + + test("homo_sapiens - [cram, crai], fasta, []") { + + when { + process { + """ + input[0] = [ + [ id: 'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram_crai'], checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.cram[0][1]).name, + process.out.bam, + process.out.sam, + process.out.bai, + process.out.crai, + process.out.csi, + process.out.versions + ).match() } + ) + } + + } + + test("homo_sapiens - [cram, []], fasta, [] - bam output") { + + config "./bam.config" + + when { + process { + """ + input[0] = [ + [ id: 'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true), + [] + ] + input[1] = [ + [ id:'genome' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.cram, + process.out.sam, + process.out.bai, + process.out.crai, + process.out.csi, + process.out.versions + ).match() } + ) + } + + } + + test("homo_sapiens - [cram, []], fasta, [] - bam & index output") { + + config "./bam_index.config" + + when { + process { + """ + input[0] = [ + [ id: 'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true), + [] + ] + input[1] = [ + [ id:'genome' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.cram, + process.out.sam, + file(process.out.csi[0][1]).name, + process.out.crai, + process.out.bai, + process.out.versions + ).match() } + ) + } + + } + + test("homo_sapiens - [cram, []], fasta, qname - bam & index output") { + + config "./bam_index.config" + + when { + process { + """ + input[0] = [ + [ id: 'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true), + [] + ] + input[1] = [ + [ id:'genome' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = Channel.of("testN:2817", "testN:2814").collectFile(name: "readnames.list", newLine: true) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.cram, + process.out.sam, + file(process.out.csi[0][1]).name, + process.out.crai, + process.out.bai, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [bam, []], [], [] - stub") { + + options "-stub" + config "./bam_index.config" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true), + [] + ] + input[1] = [[],[]] + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.cram, + process.out.sam, + file(process.out.csi[0][1]).name, + process.out.crai, + process.out.bai, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/samtools/view/tests/main.nf.test.snap b/modules/nf-core/samtools/view/tests/main.nf.test.snap new file mode 100644 index 00000000..83427491 --- /dev/null +++ b/modules/nf-core/samtools/view/tests/main.nf.test.snap @@ -0,0 +1,140 @@ +{ + "homo_sapiens - [cram, []], fasta, [] - bam output": { + "content": [ + "test.bam", + [ + + ], + [ + + ], + [ + + ], + [ + + ], + [ + + ], + [ + "versions.yml:md5,06b9049228b111e7bed5c52fe8a98d9b" + ] + ], + "timestamp": "2023-12-04T17:41:17.563069206" + }, + "sarscov2 - [bam, []], [], []": { + "content": [ + "test.bam", + [ + + ], + [ + + ], + [ + + ], + [ + + ], + [ + + ], + [ + "versions.yml:md5,06b9049228b111e7bed5c52fe8a98d9b" + ] + ], + "timestamp": "2023-12-04T17:41:03.206994564" + }, + "homo_sapiens - [cram, []], fasta, qname - bam & index output": { + "content": [ + "test.bam", + [ + + ], + [ + + ], + "test.bam.csi", + [ + + ], + [ + + ], + [ + "versions.yml:md5,06b9049228b111e7bed5c52fe8a98d9b" + ] + ], + "timestamp": "2023-12-04T17:44:39.165289759" + }, + "homo_sapiens - [cram, []], fasta, [] - bam & index output": { + "content": [ + "test.bam", + [ + + ], + [ + + ], + "test.bam.csi", + [ + + ], + [ + + ], + [ + "versions.yml:md5,06b9049228b111e7bed5c52fe8a98d9b" + ] + ], + "timestamp": "2023-12-04T17:44:32.25731224" + }, + "sarscov2 - [bam, []], [], [] - stub": { + "content": [ + "test.bam", + [ + + ], + [ + + ], + "test.csi", + [ + + ], + [ + + ], + [ + "versions.yml:md5,06b9049228b111e7bed5c52fe8a98d9b" + ] + ], + "timestamp": "2023-12-04T17:44:45.81037195" + }, + "homo_sapiens - [cram, crai], fasta, []": { + "content": [ + "test.cram", + [ + + ], + [ + + ], + [ + + ], + [ + + ], + [ + + ], + [ + "versions.yml:md5,06b9049228b111e7bed5c52fe8a98d9b" + ] + ], + "timestamp": "2023-12-04T17:41:10.730011823" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/view/tests/tags.yml b/modules/nf-core/samtools/view/tests/tags.yml new file mode 100644 index 00000000..4fdf1dd1 --- /dev/null +++ b/modules/nf-core/samtools/view/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/view: + - "modules/nf-core/samtools/view/**" diff --git a/modules/nf-core/sentieon/bwaindex/environment.yml b/modules/nf-core/sentieon/bwaindex/environment.yml new file mode 100644 index 00000000..ce0a85e4 --- /dev/null +++ b/modules/nf-core/sentieon/bwaindex/environment.yml @@ -0,0 +1,7 @@ +name: sentieon_bwaindex +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::sentieon=202308.01 diff --git a/modules/nf-core/sentieon/bwamem/environment.yml b/modules/nf-core/sentieon/bwamem/environment.yml new file mode 100644 index 00000000..c090bfa5 --- /dev/null +++ b/modules/nf-core/sentieon/bwamem/environment.yml @@ -0,0 +1,7 @@ +name: sentieon_bwamem +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::sentieon=202308.01 diff --git a/modules/nf-core/sentieon/datametrics/environment.yml b/modules/nf-core/sentieon/datametrics/environment.yml new file mode 100644 index 00000000..df094207 --- /dev/null +++ b/modules/nf-core/sentieon/datametrics/environment.yml @@ -0,0 +1,7 @@ +name: sentieon_datametrics +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::sentieon=202308.01 diff --git a/modules/nf-core/sentieon/dedup/environment.yml b/modules/nf-core/sentieon/dedup/environment.yml new file mode 100644 index 00000000..622cf739 --- /dev/null +++ b/modules/nf-core/sentieon/dedup/environment.yml @@ -0,0 +1,7 @@ +name: sentieon_dedup +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::sentieon=202308.01 diff --git a/modules/nf-core/sentieon/dnamodelapply/environment.yml b/modules/nf-core/sentieon/dnamodelapply/environment.yml new file mode 100644 index 00000000..6d27d44a --- /dev/null +++ b/modules/nf-core/sentieon/dnamodelapply/environment.yml @@ -0,0 +1,7 @@ +name: sentieon_dnamodelapply +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::sentieon=202308.01 diff --git a/modules/nf-core/sentieon/dnascope/environment.yml b/modules/nf-core/sentieon/dnascope/environment.yml new file mode 100644 index 00000000..45c2116c --- /dev/null +++ b/modules/nf-core/sentieon/dnascope/environment.yml @@ -0,0 +1,7 @@ +name: sentieon_dnascope +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::sentieon=202308.01 diff --git a/modules/nf-core/sentieon/readwriter/environment.yml b/modules/nf-core/sentieon/readwriter/environment.yml new file mode 100644 index 00000000..67dd1505 --- /dev/null +++ b/modules/nf-core/sentieon/readwriter/environment.yml @@ -0,0 +1,7 @@ +name: sentieon_readwriter +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::sentieon=202308.01 diff --git a/modules/nf-core/sentieon/wgsmetrics/environment.yml b/modules/nf-core/sentieon/wgsmetrics/environment.yml new file mode 100644 index 00000000..24878e29 --- /dev/null +++ b/modules/nf-core/sentieon/wgsmetrics/environment.yml @@ -0,0 +1,7 @@ +name: sentieon_wgsmetrics +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::sentieon=202308.01 diff --git a/modules/nf-core/smncopynumbercaller/environment.yml b/modules/nf-core/smncopynumbercaller/environment.yml new file mode 100644 index 00000000..3da92eeb --- /dev/null +++ b/modules/nf-core/smncopynumbercaller/environment.yml @@ -0,0 +1,7 @@ +name: smncopynumbercaller +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::smncopynumbercaller=1.1.2 diff --git a/modules/nf-core/stranger/environment.yml b/modules/nf-core/stranger/environment.yml new file mode 100644 index 00000000..78455053 --- /dev/null +++ b/modules/nf-core/stranger/environment.yml @@ -0,0 +1,7 @@ +name: stranger +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::stranger=0.8.1 diff --git a/modules/nf-core/svdb/merge/environment.yml b/modules/nf-core/svdb/merge/environment.yml new file mode 100644 index 00000000..4aad50da --- /dev/null +++ b/modules/nf-core/svdb/merge/environment.yml @@ -0,0 +1,9 @@ +name: svdb_merge +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - svdb=2.8.1 + # renovate: datasource=conda depName=bioconda/samtools + - samtools=1.16.1 diff --git a/modules/nf-core/tabix/bgzip/environment.yml b/modules/nf-core/tabix/bgzip/environment.yml new file mode 100644 index 00000000..4fe40c56 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/environment.yml @@ -0,0 +1,7 @@ +name: tabix_bgzip +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::tabix=1.11 diff --git a/modules/nf-core/tabix/bgziptabix/environment.yml b/modules/nf-core/tabix/bgziptabix/environment.yml new file mode 100644 index 00000000..028461c9 --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/environment.yml @@ -0,0 +1,7 @@ +name: tabix_bgziptabix +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::tabix=1.11 diff --git a/modules/nf-core/tabix/tabix/environment.yml b/modules/nf-core/tabix/tabix/environment.yml new file mode 100644 index 00000000..7167fb87 --- /dev/null +++ b/modules/nf-core/tabix/tabix/environment.yml @@ -0,0 +1,7 @@ +name: tabix_tabix +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::tabix=1.11 diff --git a/modules/nf-core/tiddit/sv/environment.yml b/modules/nf-core/tiddit/sv/environment.yml new file mode 100644 index 00000000..d0367f17 --- /dev/null +++ b/modules/nf-core/tiddit/sv/environment.yml @@ -0,0 +1,7 @@ +name: tiddit_sv +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::tiddit=3.6.1 diff --git a/modules/nf-core/ucsc/wigtobigwig/environment.yml b/modules/nf-core/ucsc/wigtobigwig/environment.yml new file mode 100644 index 00000000..3e88a5dd --- /dev/null +++ b/modules/nf-core/ucsc/wigtobigwig/environment.yml @@ -0,0 +1,7 @@ +name: ucsc_wigtobigwig +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::ucsc-wigtobigwig=447 diff --git a/modules/nf-core/untar/environment.yml b/modules/nf-core/untar/environment.yml new file mode 100644 index 00000000..0c9cbb10 --- /dev/null +++ b/modules/nf-core/untar/environment.yml @@ -0,0 +1,11 @@ +name: untar + +channels: + - conda-forge + - bioconda + - defaults + +dependencies: + - conda-forge::grep=3.11 + - conda-forge::sed=4.7 + - conda-forge::tar=1.34 diff --git a/modules/nf-core/untar/tests/main.nf.test b/modules/nf-core/untar/tests/main.nf.test new file mode 100644 index 00000000..679e83c7 --- /dev/null +++ b/modules/nf-core/untar/tests/main.nf.test @@ -0,0 +1,55 @@ +nextflow_process { + + name "Test Process UNTAR" + script "../main.nf" + process "UNTAR" + + tag "modules" + tag "modules_nfcore" + tag "untar" + + test("test_untar") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ [], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/db/kraken2.tar.gz', checkIfExists: true) ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.untar).match("test_untar") }, + ) + } + + } + + test("test_untar_onlyfiles") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ [], file(params.modules_testdata_base_path + 'generic/tar/hello.tar.gz', checkIfExists: true) ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.untar).match("test_untar_onlyfiles") }, + ) + } + + } + +} diff --git a/modules/nf-core/untar/tests/main.nf.test.snap b/modules/nf-core/untar/tests/main.nf.test.snap new file mode 100644 index 00000000..ace42576 --- /dev/null +++ b/modules/nf-core/untar/tests/main.nf.test.snap @@ -0,0 +1,34 @@ +{ + "test_untar_onlyfiles": { + "content": [ + [ + [ + [ + + ], + [ + "hello.txt:md5,e59ff97941044f85df5297e1c302d260" + ] + ] + ] + ], + "timestamp": "2023-10-18T11:56:46.878844" + }, + "test_untar": { + "content": [ + [ + [ + [ + + ], + [ + "hash.k2d:md5,8b8598468f54a7087c203ad0190555d9", + "opts.k2d:md5,a033d00cf6759407010b21700938f543", + "taxo.k2d:md5,094d5891cdccf2f1468088855c214b2c" + ] + ] + ] + ], + "timestamp": "2023-10-18T11:56:08.16574" + } +} \ No newline at end of file diff --git a/modules/nf-core/untar/tests/tags.yml b/modules/nf-core/untar/tests/tags.yml new file mode 100644 index 00000000..feb6f15c --- /dev/null +++ b/modules/nf-core/untar/tests/tags.yml @@ -0,0 +1,2 @@ +untar: + - modules/nf-core/untar/** diff --git a/modules/nf-core/upd/environment.yml b/modules/nf-core/upd/environment.yml new file mode 100644 index 00000000..77b65227 --- /dev/null +++ b/modules/nf-core/upd/environment.yml @@ -0,0 +1,7 @@ +name: upd +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::upd=0.1.1 diff --git a/modules/nf-core/vcfanno/environment.yml b/modules/nf-core/vcfanno/environment.yml new file mode 100644 index 00000000..f336cc6b --- /dev/null +++ b/modules/nf-core/vcfanno/environment.yml @@ -0,0 +1,7 @@ +name: vcfanno +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::vcfanno=0.3.5 diff --git a/modules/nf-core/vcfanno/tests/main.nf.test b/modules/nf-core/vcfanno/tests/main.nf.test new file mode 100644 index 00000000..b28431b2 --- /dev/null +++ b/modules/nf-core/vcfanno/tests/main.nf.test @@ -0,0 +1,106 @@ +nextflow_process { + + name "Test Process VCFANNO" + script "../main.nf" + process "VCFANNO" + + tag "modules" + tag "modules_nfcore" + tag "vcfanno" + + test("sarscov2 - [vcf(gz), tbi, vcf], [], toml, [vcf, tbi]") { + + when { + process { + """ + input[0] = [ + [ id:'test_compressed', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test2_vcf'], checkIfExists:true) + ] + input[1] = file(params.test_data['homo_sapiens']['genome']['vcfanno_toml'], checkIfExists: true) + input[2] = [] + input[3] = [ + file("https://github.com/brentp/vcfanno/raw/master/example/exac.vcf.gz", checkIfExists: true), + file("https://github.com/brentp/vcfanno/raw/master/example/exac.vcf.gz.tbi",checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf, [], vcf], [], toml, [vcf, tbi]") { + + when { + process { + """ + input[0] = [ + [ id:'test_uncompressed', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true), + [], + file(params.test_data['sarscov2']['illumina']['test2_vcf'], checkIfExists:true) + ] + input[1] = file(params.test_data['homo_sapiens']['genome']['vcfanno_toml'], checkIfExists: true) + input[2] = [] + input[3] = [ + file("https://github.com/brentp/vcfanno/raw/master/example/exac.vcf.gz", checkIfExists: true), + file("https://github.com/brentp/vcfanno/raw/master/example/exac.vcf.gz.tbi",checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf(gz), tbi, vcf], [], toml, [vcf, tbi] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test_compressed', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test2_vcf'], checkIfExists:true) + ] + input[1] = file(params.test_data['homo_sapiens']['genome']['vcfanno_toml'], checkIfExists: true) + input[2] = [] + input[3] = [ + file("https://github.com/brentp/vcfanno/raw/master/example/exac.vcf.gz", checkIfExists: true), + file("https://github.com/brentp/vcfanno/raw/master/example/exac.vcf.gz.tbi",checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.vcf[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/vcfanno/tests/main.nf.test.snap b/modules/nf-core/vcfanno/tests/main.nf.test.snap new file mode 100644 index 00000000..7e5f737c --- /dev/null +++ b/modules/nf-core/vcfanno/tests/main.nf.test.snap @@ -0,0 +1,73 @@ +{ + "sarscov2 - [vcf(gz), tbi, vcf], [], toml, [vcf, tbi] - stub": { + "content": [ + "test_compressed.vcf", + [ + "versions.yml:md5,5ff0991b612706ce15d82eb1564513b0" + ] + ], + "timestamp": "2023-12-06T12:18:25.69588598" + }, + "sarscov2 - [vcf(gz), tbi, vcf], [], toml, [vcf, tbi]": { + "content": [ + { + "0": [ + [ + { + "id": "test_compressed", + "single_end": false + }, + "test_compressed.vcf:md5,d3cf5a6eaf6cca5b957833a313c5fbf4" + ] + ], + "1": [ + "versions.yml:md5,5ff0991b612706ce15d82eb1564513b0" + ], + "vcf": [ + [ + { + "id": "test_compressed", + "single_end": false + }, + "test_compressed.vcf:md5,d3cf5a6eaf6cca5b957833a313c5fbf4" + ] + ], + "versions": [ + "versions.yml:md5,5ff0991b612706ce15d82eb1564513b0" + ] + } + ], + "timestamp": "2023-12-06T12:21:13.209704154" + }, + "sarscov2 - [vcf, [], vcf], [], toml, [vcf, tbi]": { + "content": [ + { + "0": [ + [ + { + "id": "test_uncompressed", + "single_end": false + }, + "test_uncompressed.vcf:md5,d3cf5a6eaf6cca5b957833a313c5fbf4" + ] + ], + "1": [ + "versions.yml:md5,5ff0991b612706ce15d82eb1564513b0" + ], + "vcf": [ + [ + { + "id": "test_uncompressed", + "single_end": false + }, + "test_uncompressed.vcf:md5,d3cf5a6eaf6cca5b957833a313c5fbf4" + ] + ], + "versions": [ + "versions.yml:md5,5ff0991b612706ce15d82eb1564513b0" + ] + } + ], + "timestamp": "2023-12-06T12:21:19.255212216" + } +} \ No newline at end of file diff --git a/modules/nf-core/vcfanno/tests/nextflow.config b/modules/nf-core/vcfanno/tests/nextflow.config new file mode 100644 index 00000000..af28ad82 --- /dev/null +++ b/modules/nf-core/vcfanno/tests/nextflow.config @@ -0,0 +1,4 @@ +process { + + +} \ No newline at end of file diff --git a/modules/nf-core/vcfanno/tests/tags.yml b/modules/nf-core/vcfanno/tests/tags.yml new file mode 100644 index 00000000..e861bd53 --- /dev/null +++ b/modules/nf-core/vcfanno/tests/tags.yml @@ -0,0 +1,2 @@ +vcfanno: + - "modules/nf-core/vcfanno/**" From 38340bae3b533c085282d2f18f4e8eb34c4bb038 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 25 Jan 2024 11:53:20 +0100 Subject: [PATCH 271/387] update subworkflows --- subworkflows/local/call_mobile_elements.nf | 2 +- subworkflows/local/call_repeat_expansions.nf | 2 +- subworkflows/local/variant_calling/call_sv_manta.nf | 4 ++-- subworkflows/local/variant_evaluation.nf | 2 +- 4 files changed, 5 insertions(+), 5 deletions(-) diff --git a/subworkflows/local/call_mobile_elements.nf b/subworkflows/local/call_mobile_elements.nf index 05e6355c..000b68cb 100644 --- a/subworkflows/local/call_mobile_elements.nf +++ b/subworkflows/local/call_mobile_elements.nf @@ -76,7 +76,7 @@ workflow CALL_MOBILE_ELEMENTS { // Fix the vcf by adding header, sorting and indexing BCFTOOLS_REHEADER_ME ( - RETROSEQ_CALL.out.vcf.map{ meta, vcf -> [ meta, vcf, [] ] }, + RETROSEQ_CALL.out.vcf.map{ meta, vcf -> [ meta, vcf, [], [] ] }, ch_genome_fai ) BCFTOOLS_SORT_ME ( BCFTOOLS_REHEADER_ME.out.vcf ) diff --git a/subworkflows/local/call_repeat_expansions.nf b/subworkflows/local/call_repeat_expansions.nf index 8e527eab..1fd76cb9 100644 --- a/subworkflows/local/call_repeat_expansions.nf +++ b/subworkflows/local/call_repeat_expansions.nf @@ -33,7 +33,7 @@ workflow CALL_REPEAT_EXPANSIONS { // Fix header and rename sample BCFTOOLS_REHEADER_EXP ( - EXPANSIONHUNTER.out.vcf.map{ meta, vcf -> [ meta, vcf, [] ]}, + EXPANSIONHUNTER.out.vcf.map{ meta, vcf -> [ meta, vcf, [], [] ]}, ch_genome_fai ) RENAMESAMPLE_EXP ( BCFTOOLS_REHEADER_EXP.out.vcf ) diff --git a/subworkflows/local/variant_calling/call_sv_manta.nf b/subworkflows/local/variant_calling/call_sv_manta.nf index 818da23c..06b5f086 100644 --- a/subworkflows/local/variant_calling/call_sv_manta.nf +++ b/subworkflows/local/variant_calling/call_sv_manta.nf @@ -32,13 +32,13 @@ workflow CALL_SV_MANTA { .combine(bai_file_list) .map { it -> it + [ [], [] ] } .set { manta_input } - MANTA ( manta_input, ch_genome_fasta, ch_genome_fai ) + MANTA ( manta_input, ch_genome_fasta, ch_genome_fai, [] ) } else { ch_case_info.combine(bam_file_list) .combine(bai_file_list) .combine(bed_input) .set { manta_input } - MANTA ( manta_input, ch_genome_fasta, ch_genome_fai ) + MANTA ( manta_input, ch_genome_fasta, ch_genome_fai, [] ) } ch_versions = MANTA.out.versions diff --git a/subworkflows/local/variant_evaluation.nf b/subworkflows/local/variant_evaluation.nf index 435b05d8..af4ed2a2 100644 --- a/subworkflows/local/variant_evaluation.nf +++ b/subworkflows/local/variant_evaluation.nf @@ -22,7 +22,7 @@ workflow VARIANT_EVALUATION { .map { row -> evregions = row.evaluationregions[0].isEmpty() ? [] : row.evaluationregions[0] bedregions = row.bedregions[0].isEmpty() ? [] : row.bedregions[0] - return [[samplename:row.samplename[0], bedregions:bedregions, evaluationregions:evregions], row.vcf[0], []] + return [[samplename:row.samplename[0], bedregions:bedregions, evaluationregions:evregions], row.vcf[0], [], []] } .set { ch_rtgvcfs_dbs } From 2b76d6b0fedb3828f8adebc18538cfca7110b6c2 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 25 Jan 2024 12:02:14 +0100 Subject: [PATCH 272/387] fix lint --- .devcontainer/devcontainer.json | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe3..4a9bc5c7 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,11 +18,11 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.linting.pylintPath": "/opt/conda/bin/pylint", }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] - } - } + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], + }, + }, } From c9d8960570e6d052e5c65f222cb83ace6795944b Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 25 Jan 2024 15:54:54 +0100 Subject: [PATCH 273/387] updates output documentation --- CHANGELOG.md | 1 + docs/output.md | 32 ++++++++++++++++++-------------- 2 files changed, 19 insertions(+), 14 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 309a21ce..2a7922da 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -46,6 +46,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Change hardcoded platform value to params.platform in align_MT.config [#475](https://github.com/nf-core/raredisease/pull/475) - The split into clincial and research VCFs is now done before ranking the varaints [#485](https://github.com/nf-core/raredisease/pull/485) - Installed the nf-core version of ensemblvep/vep module [#482](https://github.com/nf-core/raredisease/pull/482) +- The filenames of the ranked output VCF files have been changed. See [output.md](docs/output.md#filtering-and-ranking) for more information[#485](https://github.com/nf-core/raredisease/pull/485) ### `Fixed` diff --git a/docs/output.md b/docs/output.md index fd2cd30b..12edd9c4 100644 --- a/docs/output.md +++ b/docs/output.md @@ -471,28 +471,32 @@ We recommend using vcfanno to annotate SNVs with precomputed CADD scores (files -### Rank variants and filtering +### Filtering and ranking + +#### Filter_vep + +[filter_vep from VEP](https://www.ensembl.org/info/docs/tools/vep/script/vep_filter.html) is used to subset the variants based on a list of HGNC ID:s. Typpical use case is that you want to filter your results to only include variants in a predefined set of clinically relevant genes. This step is optional and can be disabled by using the flag `--skip_vep_filter`. You will always get the complete VCF together with the clinical VCF. #### GENMOD -[GENMOD](https://github.com/Clinical-Genomics/genmod) is a simple to use command line tool for annotating and analyzing genomic variations in the VCF file format. GENMOD can annotate genetic patterns of inheritance in vcf:s with single or multiple families of arbitrary size. VCF file annotated by GENMOD are further filtered using [filter_vep from VEP](https://www.ensembl.org/info/docs/tools/vep/script/vep_filter.html) to separate clinically relevant variants. You can skip the filtering step by setting --skip_vep_filter to true. +[GENMOD](https://github.com/Clinical-Genomics/genmod) is a simple to use command line tool for annotating and analyzing genomic variations in the VCF file format. GENMOD can annotate genetic patterns of inheritance in vcf:s with single or multiple families of arbitrary size. Each variant variant will be asigned a predictded pathogenicity score. The score will be given both as a raw score and a normalized score with values between 0 and 1. The tags in the INFO field are `RankScore` and `RankScoreNormalized`. The score can be configured to fit your annotations and preferences by modifying the score config file.
Output files - `rank_and_filter/` - - `_clinical_mt.vcf.gz`: file containing clinically relevant mitochondrial SNVs. - - `_clinical_mt.vcf.gz.tbi`: index of the file containing clinically relevant mitochondrial SNVs. - - `_clinical_snv.vcf.gz`: file containing clinically relevant SNVs (does not include mitochondrial variants). - - `_clinical_snv.vcf.gz.tbi`: index of the file containing clinically relevant SNVs. - - `_clinical_sv.vcf.gz`: file containing clinically relevant SVs (includes mitochondrial variants). - - `_clinical_sv.vcf.gz.tbi`: index of the file containing clinically relevant SVs. - - `_ranked_mt.vcf.gz`: file containing mitochondrial SNV annotations with their rank scores. - - `_ranked_mt.vcf.gz.tbi`: index of the file containing mitochondrial SNV annotations with their rank scores. - - `_ranked_snv.vcf.gz`: file containing SNV annotations with their rank scores (does not include mitochondrial variants). - - `_ranked_snv.vcf.gz.tbi`: index of the file containing SNV annotations with their rank scores. - - `_ranked_sv.vcf.gz`: file containing SV annotations with their rank scores (includes mitochondrial variants). - - `_ranked_sv.vcf.gz.tbi`: index of the file containing SV annotations with their rank scores. + - `_mt_ranked_clinical.vcf.gz`: file containing clinically relevant mitochondrial SNVs. + - `_mt_ranked_clinical.vcf.gz.tbi`: index of the file containing clinically relevant mitochondrial SNVs. + - `_mt_ranked_research.vcf.gz`: file containing mitochondrial SNV annotations with their rank scores. + - `_mt_ranked_research.vcf.gz.tbi`: index of the file containing mitochondrial SNV annotations with their rank scores. + - `_snv_ranked_clinical.vcf.gz`: file containing clinically relevant SNVs (does not include mitochondrial variants). + - `_snv_ranked_clinical.vcf.gz.tbi`: index of the file containing clinically relevant SNVs. + - `_snv_ranked_research.vcf.gz`: file containing SNV annotations with their rank scores (does not include mitochondrial variants). + - `_snv_ranked_research.vcf.gz.tbi`: index of the file containing SNV annotations with their rank scores. + - `_sv_ranked_clinical.vcf.gz`: file containing clinically relevant SVs (includes mitochondrial variants). + - `_sv_ranked_clinical.vcf.gz.tbi`: index of the file containing clinically relevant SVs. + - `_sv_ranked_research.vcf.gz`: file containing SV annotations with their rank scores (includes mitochondrial variants). + - `_sv_ranked_resarch.vcf.gz.tbi`: index of the file containing SV annotations with their rank scores.
From 9c7d04e377934eaf2f2f0358efba04a8c32f18a7 Mon Sep 17 00:00:00 2001 From: jemten Date: Thu, 25 Jan 2024 17:03:06 +0100 Subject: [PATCH 274/387] fixing review comments --- docs/output.md | 8 ++------ 1 file changed, 2 insertions(+), 6 deletions(-) diff --git a/docs/output.md b/docs/output.md index 12edd9c4..080c73ac 100644 --- a/docs/output.md +++ b/docs/output.md @@ -356,8 +356,6 @@ Based on VEP annotations, custom scripts used by the pipeline further annotate e - `annotate_snv/genome` - `_rohann_vcfanno_filter_vep.vcf.gz`: file containing bcftools roh, vcfanno, and vep annotations. - `_rohann_vcfanno_filter_vep.vcf.gz.tbi`: index of the file containing bcftools roh, vcfanno, and vep annotations. - - `_vep_csq_pli.vcf.gz`: file containing bcftools roh, vcfanno, vep, consequence and pli annotations. - - `_vep_csq_pli.vcf.gz.tbi`: index of the file containing bcftools roh, vcfanno, vep, consequence and pli annotations. @@ -395,8 +393,6 @@ Based on VEP annotations, custom scripts used by the pipeline further annotate e - `annotate_sv/` - `_svdbquery_vep.vcf.gz`: file containing svdb query, and vep annotations. - `_svdbquery_vep.vcf.gz.tbi`: index of the file containing bcftools roh, vcfanno, and vep annotations. - - `_vep_csq_pli.vcf.gz`: file containing bcftools roh, vcfanno, vep, consequence and pli annotations. - - `_vep_csq_pli.vcf.gz.tbi`: index of the file containing bcftools roh, vcfanno, vep, consequence and pli annotations. @@ -475,11 +471,11 @@ We recommend using vcfanno to annotate SNVs with precomputed CADD scores (files #### Filter_vep -[filter_vep from VEP](https://www.ensembl.org/info/docs/tools/vep/script/vep_filter.html) is used to subset the variants based on a list of HGNC ID:s. Typpical use case is that you want to filter your results to only include variants in a predefined set of clinically relevant genes. This step is optional and can be disabled by using the flag `--skip_vep_filter`. You will always get the complete VCF together with the clinical VCF. +[filter_vep from VEP](https://www.ensembl.org/info/docs/tools/vep/script/vep_filter.html) is used to subset the variants based on a list of HGNC ID:s. Typical use case is that you want to filter your results to only include variants in a predefined set of clinically relevant genes. This step is optional and can be disabled by using the flag `--skip_vep_filter`. You will always get the complete VCF together with the clinical VCF. #### GENMOD -[GENMOD](https://github.com/Clinical-Genomics/genmod) is a simple to use command line tool for annotating and analyzing genomic variations in the VCF file format. GENMOD can annotate genetic patterns of inheritance in vcf:s with single or multiple families of arbitrary size. Each variant variant will be asigned a predictded pathogenicity score. The score will be given both as a raw score and a normalized score with values between 0 and 1. The tags in the INFO field are `RankScore` and `RankScoreNormalized`. The score can be configured to fit your annotations and preferences by modifying the score config file. +[GENMOD](https://github.com/Clinical-Genomics/genmod) is a simple to use command line tool for annotating and analyzing genomic variations in the VCF file format. GENMOD can annotate genetic patterns of inheritance in vcf:s with single or multiple families of arbitrary size. Each variant will be assigned a predicted pathogenicity score. The score will be given both as a raw score and a normalized score with values between 0 and 1. The tags in the INFO field are `RankScore` and `RankScoreNormalized`. The score can be configured to fit your annotations and preferences by modifying the score config file.
Output files From e6973d793c70eec7344fbdf5bd46412e604d8110 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sat, 27 Jan 2024 14:16:08 +0100 Subject: [PATCH 275/387] update schema --- assets/vep_plugin_files_schema.json | 9 ++++++++- 1 file changed, 8 insertions(+), 1 deletion(-) diff --git a/assets/vep_plugin_files_schema.json b/assets/vep_plugin_files_schema.json index 6f728a7b..55695b6e 100644 --- a/assets/vep_plugin_files_schema.json +++ b/assets/vep_plugin_files_schema.json @@ -9,7 +9,14 @@ "properties": { "vep_files": { "type": "string", - "format": "file-path", + "anyOf": [ + { + "format": "file-path" + }, + { + "format": "directory-path" + } + ], "exists": true, "errorMessage": "Path to vep plugin files and their indices" } From 2ec7cf13c4fa93901655d5ba47100dc745afdaeb Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 29 Jan 2024 13:43:50 +0100 Subject: [PATCH 276/387] refactor --- conf/modules/align_MT.config | 14 +++++++------ conf/modules/align_bwa_bwamem2.config | 1 + conf/modules/annotate_cadd.config | 2 +- conf/modules/call_mobile_elements.config | 1 + conf/modules/call_repeat_expansions.config | 4 +++- conf/modules/call_snv.config | 2 +- conf/modules/call_sv_MT.config | 1 + conf/modules/convert_mt_bam_to_fastq.config | 12 +++++------ conf/modules/postprocess_MT_calls.config | 2 +- conf/modules/qc_bam.config | 4 ++++ workflows/raredisease.nf | 22 +++++++++++---------- 11 files changed, 39 insertions(+), 26 deletions(-) diff --git a/conf/modules/align_MT.config b/conf/modules/align_MT.config index cb4b55ba..e88f7e88 100644 --- a/conf/modules/align_MT.config +++ b/conf/modules/align_MT.config @@ -42,7 +42,7 @@ process { } withName: '.*ALIGN_MT:GATK4_MERGEBAMALIGNMENT_MT' { - ext.args = '--CREATE_INDEX true --MAX_GAPS -1 --SORT_ORDER queryname --INCLUDE_SECONDARY_ALIGNMENTS false --PAIRED_RUN false --VALIDATION_STRINGENCY LENIENT' + ext.args = '--TMP_DIR . --CREATE_INDEX true --MAX_GAPS -1 --SORT_ORDER queryname --INCLUDE_SECONDARY_ALIGNMENTS false --PAIRED_RUN false --VALIDATION_STRINGENCY LENIENT' ext.prefix = { "${meta.id}_merged" } } @@ -52,12 +52,13 @@ process { "--RGLB lib", "--RGPL ${params.platform}", "--RGPU barcode", - "--RGSM ${meta.id}" + "--RGSM ${meta.id}", + "--TMP_DIR ./temp_folder" ].join(' ').trim() } } withName: '.*ALIGN_MT:PICARD_MARKDUPLICATES_MT' { - ext.args = '--VALIDATION_STRINGENCY LENIENT --CREATE_INDEX true' + ext.args = '--TMP_DIR . --VALIDATION_STRINGENCY LENIENT --CREATE_INDEX true' ext.prefix = { "${meta.id}_markduplicates" } } @@ -99,7 +100,7 @@ process { } withName: '.*ALIGN_MT_SHIFT:GATK4_MERGEBAMALIGNMENT_MT' { - ext.args = '--CREATE_INDEX true --MAX_GAPS -1 --SORT_ORDER queryname --INCLUDE_SECONDARY_ALIGNMENTS false --PAIRED_RUN false --VALIDATION_STRINGENCY LENIENT' + ext.args = '--TMP_DIR . --CREATE_INDEX true --MAX_GAPS -1 --SORT_ORDER queryname --INCLUDE_SECONDARY_ALIGNMENTS false --PAIRED_RUN false --VALIDATION_STRINGENCY LENIENT' ext.prefix = { "${meta.id}_merged_shifted" } } @@ -109,12 +110,13 @@ process { "--RGLB lib", "--RGPL ${params.platform}", "--RGPU barcode", - "--RGSM ${meta.id}" + "--RGSM ${meta.id}", + "--TMP_DIR ." ].join(' ').trim() } } withName: '.*ALIGN_MT_SHIFT:PICARD_MARKDUPLICATES_MT' { - ext.args = '--VALIDATION_STRINGENCY LENIENT --CREATE_INDEX true' + ext.args = '--TMP_DIR . --VALIDATION_STRINGENCY LENIENT --CREATE_INDEX true' ext.prefix = { "${meta.id}_markduplicates_shifted" } } diff --git a/conf/modules/align_bwa_bwamem2.config b/conf/modules/align_bwa_bwamem2.config index 4d8b59e3..15453e0e 100644 --- a/conf/modules/align_bwa_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2.config @@ -40,6 +40,7 @@ process { } withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:MARKDUPLICATES' { + ext.args = "--TMP_DIR ." ext.prefix = { "${meta.id}_sorted_md" } publishDir = [ enabled: !params.save_mapped_as_cram, diff --git a/conf/modules/annotate_cadd.config b/conf/modules/annotate_cadd.config index 5cba1d6a..438beeb5 100644 --- a/conf/modules/annotate_cadd.config +++ b/conf/modules/annotate_cadd.config @@ -16,7 +16,7 @@ // process { - if (params.cadd_resources != null) { + if (!params.cadd_resources != "null") { withName: '.*:ANNOTATE_CADD.*' { ext.when = { ( !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun) } } diff --git a/conf/modules/call_mobile_elements.config b/conf/modules/call_mobile_elements.config index f3622869..0a5e4faf 100644 --- a/conf/modules/call_mobile_elements.config +++ b/conf/modules/call_mobile_elements.config @@ -38,6 +38,7 @@ process { } withName: '.*CALL_MOBILE_ELEMENTS:BCFTOOLS_REHEADER_ME' { + ext.args = "--temp-prefix ." ext.args2 = { '--output-type v' } ext.prefix = { "${meta.id}_${meta.interval}_retroseq_reheader" } } diff --git a/conf/modules/call_repeat_expansions.config b/conf/modules/call_repeat_expansions.config index 13a7e519..db12e4ff 100644 --- a/conf/modules/call_repeat_expansions.config +++ b/conf/modules/call_repeat_expansions.config @@ -24,11 +24,12 @@ process { } withName: '.*CALL_REPEAT_EXPANSIONS:EXPANSIONHUNTER' { - ext.args = { ("${meta.sex}" == 1) ? '--sex male' : '--sex female' } + ext.args = { ("${meta.sex}" == '1') ? '--sex male' : '--sex female' } ext.prefix = { "${meta.id}_exphunter" } } withName: '.*CALL_REPEAT_EXPANSIONS:BCFTOOLS_REHEADER_EXP' { + ext.args = "--temp-prefix ." ext.prefix = { "${meta.id}_reheader" } } @@ -66,6 +67,7 @@ process { withName: '.*CALL_REPEAT_EXPANSIONS:COMPRESS_STRANGER' { ext.prefix = { "${meta.id}_repeat_expansion" } + ext.args = '--output-type z' publishDir = [ path: { "${params.outdir}/repeat_expansions" }, mode: params.publish_dir_mode, diff --git a/conf/modules/call_snv.config b/conf/modules/call_snv.config index 51f7a56f..92eda8cf 100644 --- a/conf/modules/call_snv.config +++ b/conf/modules/call_snv.config @@ -20,7 +20,7 @@ process { } withName: '.*CALL_SNV:GATK4_SELECTVARIANTS' { - ext.args = "--exclude-intervals ${params.mito_name}" + ext.args = { "--exclude-intervals ${params.mito_name}" } ext.prefix = { "${meta.id}_nomito" } publishDir = [ path: { "${params.outdir}/call_snv/genome" }, diff --git a/conf/modules/call_sv_MT.config b/conf/modules/call_sv_MT.config index 606ef429..b7022829 100644 --- a/conf/modules/call_sv_MT.config +++ b/conf/modules/call_sv_MT.config @@ -28,6 +28,7 @@ process { if (!params.skip_eklipse){ withName: '.*CALL_SV_MT:EKLIPSE' { + ext.args = "-tmp ." publishDir = [ path: { "${params.outdir}/call_sv/mitochondria" }, mode: params.publish_dir_mode, diff --git a/conf/modules/convert_mt_bam_to_fastq.config b/conf/modules/convert_mt_bam_to_fastq.config index 0365c452..66c81425 100644 --- a/conf/modules/convert_mt_bam_to_fastq.config +++ b/conf/modules/convert_mt_bam_to_fastq.config @@ -18,19 +18,19 @@ process { withName: '.*ALIGN:CONVERT_MT_BAM_TO_FASTQ:GATK4_PRINTREADS_MT' { - beforeScript = {"mkdir ./tmp"} - ext.args = [ + ext.args = { [ "-L ${params.mito_name}", "--read-filter MateOnSameContigOrNoMappedMateReadFilter", - "--read-filter MateUnmappedAndUnmappedReadFilter" - ].join(" ").trim() + "--read-filter MateUnmappedAndUnmappedReadFilter", + "--tmp-dir ." + ].join(" ").trim() } } withName: '.*ALIGN:CONVERT_MT_BAM_TO_FASTQ:GATK4_REVERTSAM_MT' { - ext.args = '--OUTPUT_BY_READGROUP false --VALIDATION_STRINGENCY LENIENT --ATTRIBUTE_TO_CLEAR FT --ATTRIBUTE_TO_CLEAR CO --SORT_ORDER queryname --RESTORE_ORIGINAL_QUALITIES false' + ext.args = '--TMP_DIR . --OUTPUT_BY_READGROUP false --VALIDATION_STRINGENCY LENIENT --ATTRIBUTE_TO_CLEAR FT --ATTRIBUTE_TO_CLEAR CO --SORT_ORDER queryname --RESTORE_ORIGINAL_QUALITIES false' } withName: '.*ALIGN:CONVERT_MT_BAM_TO_FASTQ:GATK4_SAMTOFASTQ_MT' { - ext.args = '--VALIDATION_STRINGENCY LENIENT' + ext.args = '--VALIDATION_STRINGENCY LENIENT --TMP_DIR .' } } diff --git a/conf/modules/postprocess_MT_calls.config b/conf/modules/postprocess_MT_calls.config index 07e266de..ba6e8abf 100644 --- a/conf/modules/postprocess_MT_calls.config +++ b/conf/modules/postprocess_MT_calls.config @@ -18,7 +18,7 @@ process { withName: '.*POSTPROCESS_MT_CALLS:GATK4_MERGEVCFS_LIFT_UNLIFT_MT' { - ext.prefix = { "${meta.id}_merged" } + ext.prefix = { "${meta.id}_merged_liftunlift" } } withName: '.*POSTPROCESS_MT_CALLS:GATK4_VARIANTFILTRATION_MT' { diff --git a/conf/modules/qc_bam.config b/conf/modules/qc_bam.config index 1982a757..61daf4d6 100644 --- a/conf/modules/qc_bam.config +++ b/conf/modules/qc_bam.config @@ -25,10 +25,12 @@ process { } withName: '.*QC_BAM:PICARD_COLLECTMULTIPLEMETRICS' { + ext.args = "--TMP_DIR ." ext.prefix = { "${meta.id}_multiplemetrics" } } withName: '.*QC_BAM:PICARD_COLLECTHSMETRICS' { + ext.args = "--TMP_DIR ." ext.when = { !params.target_bed.equals(null) } ext.prefix = { "${meta.id}_hsmetrics" } } @@ -65,11 +67,13 @@ process { } withName: '.*QC_BAM:PICARD_COLLECTWGSMETRICS' { + ext.args = "--TMP_DIR ." ext.when = { params.analysis_type.equals("wgs") && !params.aligner.equals("sentieon") } ext.prefix = { "${meta.id}_wgsmetrics" } } withName: '.*QC_BAM:PICARD_COLLECTWGSMETRICS_Y' { + ext.args = "--TMP_DIR ." ext.when = { params.analysis_type.equals("wgs") && !params.aligner.equals("sentieon") } ext.prefix = { "${meta.id}_wgsmetrics_y" } } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index ec55bb7f..9f402370 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -312,17 +312,19 @@ workflow RAREDISEASE { } // Read and store paths in the vep_plugin_files file - ch_vep_extra_files_unsplit.splitCsv ( header:true ) - .map { row -> - f = file(row.vep_files[0]) - if(f.isFile() || f.isDirectory()){ - return [f] - } else { - error("\nVep database file ${f} does not exist.") + if (params.vep_plugin_files) { + ch_vep_extra_files_unsplit.splitCsv ( header:true ) + .map { row -> + f = file(row.vep_files[0]) + if(f.isFile() || f.isDirectory()){ + return [f] + } else { + error("\nVep database file ${f} does not exist.") + } } - } - .collect() - .set {ch_vep_extra_files} + .collect() + .set {ch_vep_extra_files} + } // Input QC if (!params.skip_fastqc) { From a78b52c0157f3e8e101974438c7562ba9970badc Mon Sep 17 00:00:00 2001 From: lucpen Date: Mon, 29 Jan 2024 14:03:48 +0100 Subject: [PATCH 277/387] fix vepvep configs --- conf/modules/annotate_genome_snvs.config | 6 +++--- conf/modules/annotate_mobile_elements.config | 2 +- conf/modules/annotate_mt_snvs.config | 6 +++--- conf/modules/annotate_structural_variants.config | 6 +++--- 4 files changed, 10 insertions(+), 10 deletions(-) diff --git a/conf/modules/annotate_genome_snvs.config b/conf/modules/annotate_genome_snvs.config index 5b1e9928..c1bb9144 100644 --- a/conf/modules/annotate_genome_snvs.config +++ b/conf/modules/annotate_genome_snvs.config @@ -77,10 +77,10 @@ process { withName: '.*ANNOTATE_GENOME_SNVS:ENSEMBLVEP_SNV' { ext.prefix = { "${vcf.simpleName}_rohann_vcfanno_filter_vep" } - ext.args = [ + ext.args = { [ '--dir_plugins vep_cache/Plugins', '--plugin LoFtool,LoFtool_scores.txt', - '--plugin pLI,pLI_values_107.txt', + "--plugin pLI,pLI_values_${params.vep_cache_version}.txt", '--plugin SpliceAI,snv=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', '--distance 5000', '--buffer_size 20000', @@ -90,7 +90,7 @@ process { '--hgvs --humdiv --no_progress --numbers', '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl', '--uniprot --vcf' - ].join(' ') + ].join(' ') } } withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_CONCAT' { diff --git a/conf/modules/annotate_mobile_elements.config b/conf/modules/annotate_mobile_elements.config index cb62894d..e94f67af 100644 --- a/conf/modules/annotate_mobile_elements.config +++ b/conf/modules/annotate_mobile_elements.config @@ -40,7 +40,7 @@ process { ext.args = { [ '--dir_cache vep_cache', '--dir_plugins vep_cache/Plugins', - '--plugin pLI,pLI_values_107.txt', + "--plugin pLI,pLI_values_${params.vep_cache_version}.txt", '--appris --biotype --buffer_size 100 --canonical --cache --ccds', '--compress_output bgzip --distance 5000 --domains', '--exclude_predicted --force_overwrite --format vcf', diff --git a/conf/modules/annotate_mt_snvs.config b/conf/modules/annotate_mt_snvs.config index f0e46836..dfa7ea2f 100644 --- a/conf/modules/annotate_mt_snvs.config +++ b/conf/modules/annotate_mt_snvs.config @@ -18,10 +18,10 @@ process { if (!params.skip_mt_annotation) { withName: '.*ANNOTATE_MT_SNVS:ENSEMBLVEP_MT' { - ext.args = [ + ext.args = { [ '--dir_plugins vep_cache/Plugins', '--plugin LoFtool,LoFtool_scores.txt', - '--plugin pLI,pLI_values_107.txt', + "--plugin pLI,pLI_values_${params.vep_cache_version}.txt", '--plugin SpliceAI,snv=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', '--distance 0', '--buffer_size 20000', @@ -31,7 +31,7 @@ process { '--hgvs --humdiv --no_progress --numbers', '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl --vcf', '--uniprot' - ].join(' ') + ].join(' ') } } withName: '.*ANNOTATE_MT_SNVS:ZIP_TABIX_HMTNOTE' { diff --git a/conf/modules/annotate_structural_variants.config b/conf/modules/annotate_structural_variants.config index 9f8f5f19..74425f31 100644 --- a/conf/modules/annotate_structural_variants.config +++ b/conf/modules/annotate_structural_variants.config @@ -43,10 +43,10 @@ process { } withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:ENSEMBLVEP_SV' { - ext.args = [ + ext.args = { [ '--dir_cache vep_cache', '--dir_plugins vep_cache/Plugins', - '--plugin pLI,pLI_values_107.txt', + "--plugin pLI,pLI_values_${params.vep_cache_version}.txt", '--appris --biotype --buffer_size 100 --canonical --cache --ccds', '--compress_output bgzip --distance 5000 --domains', '--exclude_predicted --force_overwrite --format vcf', @@ -54,7 +54,7 @@ process { '--no_progress --numbers --per_gene --polyphen p', '--protein --offline --regulatory --sift p', '--symbol --tsl --uniprot --vcf' - ].join(' ') + ].join(' ') } ext.prefix = { "${meta.id}_svdbquery_vep" } publishDir = [ path: { "${params.outdir}/annotate_sv" }, From 0467013eb6f1d9d75c9e69670d4aa495473bff40 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 29 Jan 2024 15:13:19 +0000 Subject: [PATCH 278/387] Template update for nf-core/tools version 2.12 --- .editorconfig | 8 + .github/workflows/awsfulltest.yml | 2 +- .github/workflows/awstest.yml | 2 +- .github/workflows/branch.yml | 2 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 67 +++++ .github/workflows/fix-linting.yml | 72 +++-- .github/workflows/linting.yml | 61 +--- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 4 +- .gitpod.yml | 1 + .pre-commit-config.yaml | 7 +- README.md | 8 +- assets/email_template.html | 2 +- assets/email_template.txt | 2 +- assets/nf-core-raredisease_logo_light.png | Bin 75763 -> 122212 bytes docs/images/nf-core-raredisease_logo_dark.png | Bin 75872 -> 31960 bytes .../images/nf-core-raredisease_logo_light.png | Bin 75763 -> 26891 bytes lib/WorkflowMain.groovy | 16 +- lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes main.nf | 2 +- modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 2 +- .../custom/dumpsoftwareversions/main.nf | 4 +- .../dumpsoftwareversions/tests/main.nf.test | 7 +- .../tests/main.nf.test.snap | 50 ++-- modules/nf-core/fastqc/tests/main.nf.test | 265 ++++++++++++------ .../nf-core/fastqc/tests/main.nf.test.snap | 12 +- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 6 +- modules/nf-core/multiqc/meta.yml | 1 - modules/nf-core/multiqc/tests/main.nf.test | 48 +++- .../nf-core/multiqc/tests/main.nf.test.snap | 21 ++ nextflow.config | 3 +- pyproject.toml | 17 +- workflows/raredisease.nf | 7 + 36 files changed, 490 insertions(+), 221 deletions(-) create mode 100644 .github/workflows/download_pipeline.yml delete mode 100644 lib/nfcore_external_java_deps.jar create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap diff --git a/.editorconfig b/.editorconfig index b6b31907..9b990088 100644 --- a/.editorconfig +++ b/.editorconfig @@ -22,3 +22,11 @@ indent_size = unset [/assets/email*] indent_size = unset + +# ignore Readme +[README.md] +indent_style = unset + +# ignore python +[*.{py}] +indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 929ad504..2919151f 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 9a6e057a..4ed98bab 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 387c04ca..54998898 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v1 + uses: mshick/add-pr-comment@v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index 694e90ec..e37cfda5 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v7 + - uses: actions/stale@v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml new file mode 100644 index 00000000..8611458a --- /dev/null +++ b/.github/workflows/download_pipeline.yml @@ -0,0 +1,67 @@ +name: Test successful pipeline download with 'nf-core download' + +# Run the workflow when: +# - dispatched manually +# - when a PR is opened or reopened to master branch +# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. +on: + workflow_dispatch: + pull_request: + types: + - opened + branches: + - master + pull_request_target: + branches: + - master + +env: + NXF_ANSI_LOG: false + +jobs: + download: + runs-on: ubuntu-latest + steps: + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + + - uses: actions/setup-python@v5 + with: + python-version: "3.11" + architecture: "x64" + - uses: eWaterCycle/setup-singularity@v7 + with: + singularity-version: 3.8.3 + + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install git+https://github.com/nf-core/tools.git@dev + + - name: Get the repository name and current branch set as environment variable + run: | + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + + - name: Download the pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + run: | + nf-core download ${{ env.REPO_LOWERCASE }} \ + --revision ${{ env.REPO_BRANCH }} \ + --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ + --compress "none" \ + --container-system 'singularity' \ + --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-cache-utilisation 'amend' \ + --download-configuration + + - name: Inspect download + run: tree ./${{ env.REPOTITLE_LOWERCASE }} + + - name: Run the downloaded pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index b7ece6b1..114b102d 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -4,7 +4,7 @@ on: types: [created] jobs: - deploy: + fix-linting: # Only run if comment is on a PR with the main repo, and if it contains the magic keywords if: > contains(github.event.comment.html_url, '/pull/') && @@ -13,10 +13,17 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} + # indication that the linting is being fixed + - name: React on comment + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: eyes + # Action runs on the issue comment, so we don't get the PR by default # Use the gh cli to check out the PR - name: Checkout Pull Request @@ -24,32 +31,59 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v4 + # Install and run pre-commit + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + with: + python-version: 3.11 - - name: Install Prettier - run: npm install -g prettier @prettier/plugin-php + - name: Install pre-commit + run: pip install pre-commit - # Check that we actually need to fix something - - name: Run 'prettier --check' - id: prettier_status - run: | - if prettier --check ${GITHUB_WORKSPACE}; then - echo "result=pass" >> $GITHUB_OUTPUT - else - echo "result=fail" >> $GITHUB_OUTPUT - fi + - name: Run pre-commit + id: pre-commit + run: pre-commit run --all-files + continue-on-error: true - - name: Run 'prettier --write' - if: steps.prettier_status.outputs.result == 'fail' - run: prettier --write ${GITHUB_WORKSPACE} + # indication that the linting has finished + - name: react if linting finished succesfully + if: steps.pre-commit.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: "+1" - name: Commit & push changes - if: steps.prettier_status.outputs.result == 'fail' + id: commit-and-push + if: steps.pre-commit.outcome == 'failure' run: | git config user.email "core@nf-co.re" git config user.name "nf-core-bot" git config push.default upstream git add . git status - git commit -m "[automated] Fix linting with Prettier" + git commit -m "[automated] Fix code linting" git push + + - name: react if linting errors were fixed + id: react-if-fixed + if: steps.commit-and-push.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: hooray + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: confused + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + issue-number: ${{ github.event.issue.number }} + body: | + @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually. + See [CI log](https://github.com/nf-core/raredisease/actions/runs/${{ github.run_id }}) for more details. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 905c58e4..81cd098e 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,61 +11,22 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - uses: actions/checkout@v4 - - uses: actions/setup-node@v4 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - uses: actions/setup-node@v4 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.11 + uses: actions/setup-python@v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: 3.11 + cache: "pip" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest @@ -76,7 +37,7 @@ jobs: - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.11" architecture: "x64" @@ -99,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 0bbcd30f..147bcd10 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v2 + uses: dawidd6/action-download-artifact@v3 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 6ad33927..21ac3f06 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -24,7 +24,7 @@ jobs: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +56,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.0.2 + - uses: zentered/bluesky-post-action@v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/.gitpod.yml b/.gitpod.yml index acf72695..363d5b1d 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -7,6 +7,7 @@ tasks: - name: unset JAVA_TOOL_OPTIONS command: | unset JAVA_TOOL_OPTIONS + vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 0c31cdb9..af57081f 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -1,5 +1,10 @@ repos: - repo: https://github.com/pre-commit/mirrors-prettier - rev: "v2.7.1" + rev: "v3.1.0" hooks: - id: prettier + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python + rev: "2.7.3" + hooks: + - id: editorconfig-checker + alias: ec diff --git a/README.md b/README.md index 0eb4e6d5..1d2a44f5 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,9 @@ -# ![nf-core/raredisease](docs/images/nf-core-raredisease_logo_light.png#gh-light-mode-only) ![nf-core/raredisease](docs/images/nf-core-raredisease_logo_dark.png#gh-dark-mode-only) - +

+ + + nf-core/raredisease + +

[![GitHub Actions CI Status](https://github.com/nf-core/raredisease/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/raredisease/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/raredisease/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/raredisease/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/raredisease/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) diff --git a/assets/email_template.html b/assets/email_template.html index f7f3e4ef..d3a18a13 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -12,7 +12,7 @@ -

nf-core/raredisease v${version}

+

nf-core/raredisease ${version}

Run Name: $runName

<% if (!success){ diff --git a/assets/email_template.txt b/assets/email_template.txt index a89246e0..5a017e04 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -4,7 +4,7 @@ |\\ | |__ __ / ` / \\ |__) |__ } { | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, `._,._,' - nf-core/raredisease v${version} + nf-core/raredisease ${version} ---------------------------------------------------- Run Name: $runName diff --git a/assets/nf-core-raredisease_logo_light.png b/assets/nf-core-raredisease_logo_light.png index 3e1c6ec69ad091015a8df756efd4bfee1d337628..163054c389f13eb2eac09d88c1aaaa5d99719db2 100644 GIT binary patch literal 122212 zcmeFY^YuTH%xWH& zN)$d9cVfh#PwW_2kP8)8Z@;r)jb-Y)6>%%U4pSY^|_ zAk#e`(CwSrI`YXHrnQ=Z# zqD})!l;V*K5c8)mn5J}92F`wt`TV{z)Nw89F$(#L^Lh7ke3F^Yc31z5ZIGml`J7u! z<$WJkftAl)UUaQaMs1H|9{9=>nRy%Jj<*K9Qb?#o zLC|nem1Sr(RL3fHM$Gbw~gqx!bt ze1#nNt&UDfrD~)%TiDIK|LlV~GbQ4F$<@}^2(5TeZeqLTPeCi=>Eyq9t6FbZJf;uk+h%$HO8R-ewrR$V2+U*@om23P;MqffI#`0Zw?jK 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Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } } diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar deleted file mode 100644 index 805c8bb5e4fd43a12a5891eea5a68788309629b0..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX zni_mKLGakt&P12{r^KCD0GPa9sIuy`fu+agt?8O-hY9i{WloGe}y@kSsDEY_Fud9 z-?2ab4J)MQXe4E3Xl-U<>PRMQZ)0m@@90KnV5#Te5Tz`pvZ0Lj4F<9%Flh(`Qb;Tn zMV!iFmH{S^fF*j#MCKRUd`7m>9||*;WE32I_6pg4-Q1C#ke$$aVmP-TtfJ+j+N9;Q z>bxN=bCVQkg$Jzty71(+~A9}ZfANuYIVnTdW!??Sj)Ti-Qpk8XgPDab8pk2QD&dwUl=iFhd1c$C2uMVagr zWc@z9s$#g__{3?VGb#)^fSNp^lZh zU>MI!D?+L)nyth{@@L0+B&_M8iR08L)G6#PZQLFmvz-9DlZkwRWigRIyI4xYr7>tRJ-N6MlBB`nJQ!!8D)mI2lB3PP;dC{<{Td>^ 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b/modules.json index 9b750add..ab7892e3 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", + "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "4ab13872435962dadc239979554d13709e20bf29", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index f0c63f69..9b3272bc 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.17 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 7685b33c..f2187611 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test index eec1db10..b1e1630b 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -31,7 +31,12 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } ) } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap index 4274ed57..5f59a936 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -1,27 +1,33 @@ { "Should run without failures": { "content": [ - { - "0": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ], - "1": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "2": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "mqc_yml": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "versions": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "yml": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ] - } + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n

Process Name \\", + " \\ Software Version
CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
yaml5.4.1
TOOL1tool10.11.9
TOOL2tool21.9
WorkflowNextflow
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File typeConventional base calls
File typeConventional base calls
\\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] ], - "timestamp": "2023-11-03T14:43:22.157011" + "timestamp": "2024-01-09T23:01:18.710682" } -} +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index b9e8f926..1f21c664 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -3,24 +3,20 @@ nextflow_process { name "Test Process FASTQC" script "../main.nf" process "FASTQC" + tag "modules" tag "modules_nfcore" tag "fastqc" - test("Single-Read") { + test("sarscov2 single-end [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ - input[0] = [ + input[0] = Channel.of([ [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) """ } } @@ -28,82 +24,189 @@ nextflow_process { then { assertAll ( { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. // looks like this:
Mon 2 Oct 2023
test.gz
// https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, - { assert path(process.out.html.get(0).get(1)).getText().contains("") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 paired-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 interleaved [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } ) } } -// TODO -// // -// // Test with paired-end data -// // -// workflow test_fastqc_paired_end { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with interleaved data -// // -// workflow test_fastqc_interleaved { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with bam data -// // -// workflow test_fastqc_bam { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with multiple samples -// // -// workflow test_fastqc_multiple { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with custom prefix -// // -// workflow test_fastqc_custom_prefix { -// input = [ -// [ id:'mysample', single_end:true ], // meta map -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } + + test("sarscov2 paired-end [bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 multiple [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + { assert path(process.out.html[0][1][2]).text.contains("") }, + { assert path(process.out.html[0][1][3]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 custom_prefix") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastq] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.html.collect { file(it[1]).getName() } + + process.out.zip.collect { file(it[1]).getName() } + + process.out.versions ).match() } + ) + } + } + } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 636a32ce..5d624bb8 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,10 +1,20 @@ { + "sarscov2 single-end [fastq] - stub": { + "content": [ + [ + "test.html", + "test.zip", + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2024-01-17T18:40:57.254299" + }, "versions": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2023-10-09T23:40:54+0000" + "timestamp": "2024-01-17T18:36:50.033627" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index bc0bdb5b..7625b752 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.18 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 00cc48d2..1b9f7c43 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : - 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -43,7 +43,7 @@ process MULTIQC { stub: """ - touch multiqc_data + mkdir multiqc_data touch multiqc_plots touch multiqc_report.html diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f1aa660e..45a9bc35 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,4 +1,3 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index c2dad217..d0438eda 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -7,12 +7,9 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" - test("MULTIQC: FASTQC") { + test("sarscov2 single-end [fastqc]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -26,20 +23,17 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } ) } } - test("MULTIQC: FASTQC and a config file") { + test("sarscov2 single-end [fastqc] [config]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -53,9 +47,35 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastqc] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.report.collect { file(it).getName() } + + process.out.data.collect { file(it).getName() } + + process.out.plots.collect { file(it).getName() } + + process.out.versions ).match() } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap new file mode 100644 index 00000000..d37e7304 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -0,0 +1,21 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:02:49.911994" + }, + "sarscov2 single-end [fastqc] - stub": { + "content": [ + [ + "multiqc_report.html", + "multiqc_data", + "multiqc_plots", + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:03:14.524346" + } +} \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 9e9e442b..2d608ff7 100644 --- a/nextflow.config +++ b/nextflow.config @@ -71,7 +71,7 @@ try { } // Load nf-core/raredisease custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs! +// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! // try { // includeConfig "${params.custom_config_base}/pipeline/raredisease.config" // } catch (Exception e) { @@ -91,6 +91,7 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false + channels = ['conda-forge', 'bioconda', 'defaults'] apptainer.enabled = false } mamba { diff --git a/pyproject.toml b/pyproject.toml index 0d62beb6..7d08e1c8 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,10 +1,13 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] +[tool.ruff] line-length = 120 -target_version = ["py37", "py38", "py39", "py310"] +target-version = "py38" +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] +cache-dir = "~/.cache/ruff" -[tool.isort] -profile = "black" -known_first_party = ["nf_core"] -multi_line_output = 3 +[tool.ruff.isort] +known-first-party = ["nf_core"] + +[tool.ruff.per-file-ignores] +"__init__.py" = ["E402", "F401"] diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index eed8d648..ac152bea 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -127,6 +127,13 @@ workflow.onComplete { } } +workflow.onError { + if (workflow.errorReport.contains("Process requirement exceeds available memory")) { + println("🛑 Default resources exceed availability 🛑 ") + println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") + } +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END From 17860c92d69a402bc15bd26f87bee5926e3ecf49 Mon Sep 17 00:00:00 2001 From: Anders Jemt Date: Mon, 29 Jan 2024 17:36:31 +0100 Subject: [PATCH 279/387] fix input channel for vcf2cytosure subworkflow --- subworkflows/local/generate_cytosure_files.nf | 10 +++++++--- workflows/raredisease.nf | 3 ++- 2 files changed, 9 insertions(+), 4 deletions(-) diff --git a/subworkflows/local/generate_cytosure_files.nf b/subworkflows/local/generate_cytosure_files.nf index 3d716f92..561411ab 100644 --- a/subworkflows/local/generate_cytosure_files.nf +++ b/subworkflows/local/generate_cytosure_files.nf @@ -8,7 +8,8 @@ include { VCF2CYTOSURE } from '../../modules/nf-core workflow GENERATE_CYTOSURE_FILES { take: - ch_vcf // channel: [mandatory] [ val(meta), path(vcf), path(vcf_index) ] + ch_vcf // channel: [mandatory] [ val(meta), path(vcf) ] + ch_tbi // channel: [mandatory] [ val(meta), path(vcf_index) ] ch_bam // channel: [mandatory] [ val(meta), path(bam) ] ch_blacklist // channel: [optional] [path(blacklist)] @@ -17,8 +18,11 @@ workflow GENERATE_CYTOSURE_FILES { TIDDIT_COV_VCF2CYTOSURE (ch_bam, [[],[]]) - // Build channel: [val(sample_meta). path(vcf), path(vcf_index)] - ch_bam.combine(ch_vcf).map { + // Build channel: [val(sample_meta), path(vcf), path(vcf_index)] + ch_vcf.join( ch_tbi, failOnMismatch: true ) + .set { ch_vcf_tbi } + + ch_bam.combine(ch_vcf_tbi).map { meta_sample, bam, meta_case, vcf, tbi -> return [ meta_sample, vcf, tbi ] }.set { ch_sample_vcf } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index ec55bb7f..54fe83a8 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -610,7 +610,8 @@ workflow RAREDISEASE { // Generate CGH files from sequencing data, turned off by default if ( !params.skip_vcf2cytosure && params.analysis_type != "wes" ) { GENERATE_CYTOSURE_FILES ( - BGZIPTABIX_SV.out.gz_tbi, + ch_sv_annotate.vcf_ann, + ch_sv_annotate.tbi, ch_mapped.genome_marked_bam, ch_vcf2cytosure_blacklist ) From 1fb1d91c4511caa1f976710e110181acb7b22f42 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 29 Jan 2024 23:09:00 +0100 Subject: [PATCH 280/387] add genomecov --- modules.json | 5 + .../bedtools/genomecov/environment.yml | 7 ++ modules/nf-core/bedtools/genomecov/main.nf | 70 +++++++++++ modules/nf-core/bedtools/genomecov/meta.yml | 59 +++++++++ .../bedtools/genomecov/tests/main.nf.test | 118 ++++++++++++++++++ .../genomecov/tests/main.nf.test.snap | 95 ++++++++++++++ .../bedtools/genomecov/tests/nextflow.config | 7 ++ .../nf-core/bedtools/genomecov/tests/tags.yml | 2 + 8 files changed, 363 insertions(+) create mode 100644 modules/nf-core/bedtools/genomecov/environment.yml create mode 100644 modules/nf-core/bedtools/genomecov/main.nf create mode 100644 modules/nf-core/bedtools/genomecov/meta.yml create mode 100644 modules/nf-core/bedtools/genomecov/tests/main.nf.test create mode 100644 modules/nf-core/bedtools/genomecov/tests/main.nf.test.snap create mode 100644 modules/nf-core/bedtools/genomecov/tests/nextflow.config create mode 100644 modules/nf-core/bedtools/genomecov/tests/tags.yml diff --git a/modules.json b/modules.json index e33ea340..e86e65d7 100644 --- a/modules.json +++ b/modules.json @@ -50,6 +50,11 @@ "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, + "bedtools/genomecov": { + "branch": "master", + "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", + "installed_by": ["modules"] + }, "bwa/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", diff --git a/modules/nf-core/bedtools/genomecov/environment.yml b/modules/nf-core/bedtools/genomecov/environment.yml new file mode 100644 index 00000000..8fbe20c3 --- /dev/null +++ b/modules/nf-core/bedtools/genomecov/environment.yml @@ -0,0 +1,7 @@ +name: bedtools_genomecov +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bedtools=2.31.1 diff --git a/modules/nf-core/bedtools/genomecov/main.nf b/modules/nf-core/bedtools/genomecov/main.nf new file mode 100644 index 00000000..7a4d9c45 --- /dev/null +++ b/modules/nf-core/bedtools/genomecov/main.nf @@ -0,0 +1,70 @@ +process BEDTOOLS_GENOMECOV { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bedtools:2.31.1--hf5e1c6e_0' : + 'biocontainers/bedtools:2.31.1--hf5e1c6e_0' }" + + input: + tuple val(meta), path(intervals), val(scale) + path sizes + val extension + + output: + tuple val(meta), path("*.${extension}"), emit: genomecov + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args_list = args.tokenize() + args += (scale > 0 && scale != 1) ? " -scale $scale" : "" + if (!args_list.contains('-bg') && (scale > 0 && scale != 1)) { + args += " -bg" + } + + def prefix = task.ext.prefix ?: "${meta.id}" + if (intervals.name =~ /\.bam/) { + """ + bedtools \\ + genomecov \\ + -ibam $intervals \\ + $args \\ + > ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ + } else { + """ + bedtools \\ + genomecov \\ + -i $intervals \\ + -g $sizes \\ + $args \\ + > ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ + } + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/bedtools/genomecov/meta.yml b/modules/nf-core/bedtools/genomecov/meta.yml new file mode 100644 index 00000000..2b2385e3 --- /dev/null +++ b/modules/nf-core/bedtools/genomecov/meta.yml @@ -0,0 +1,59 @@ +name: bedtools_genomecov +description: Computes histograms (default), per-base reports (-d) and BEDGRAPH (-bg) summaries of feature coverage (e.g., aligned sequences) for a given genome. +keywords: + - bed + - bam + - genomecov + - bedtools + - histogram +tools: + - bedtools: + description: | + A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. + documentation: https://bedtools.readthedocs.io/en/latest/content/tools/genomecov.html + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - intervals: + type: file + description: BAM/BED/GFF/VCF + pattern: "*.{bam|bed|gff|vcf}" + - scale: + type: integer + description: Number containing the scale factor for the output. Set to 1 to disable. Setting to a value other than 1 will also get the -bg bedgraph output format as this is required for this command switch + - sizes: + type: file + description: Tab-delimited table of chromosome names in the first column and chromosome sizes in the second column + - extension: + type: string + description: Extension of the output file (e. g., ".bg", ".bedgraph", ".txt", ".tab", etc.) It is set arbitrarily by the user and corresponds to the file format which depends on arguments. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - genomecov: + type: file + description: Computed genome coverage file + pattern: "*.${extension}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@edmundmiller" + - "@sruthipsuresh" + - "@drpatelh" + - "@sidorov-si" + - "@chris-cheshire" +maintainers: + - "@edmundmiller" + - "@sruthipsuresh" + - "@drpatelh" + - "@sidorov-si" + - "@chris-cheshire" diff --git a/modules/nf-core/bedtools/genomecov/tests/main.nf.test b/modules/nf-core/bedtools/genomecov/tests/main.nf.test new file mode 100644 index 00000000..21e69aed --- /dev/null +++ b/modules/nf-core/bedtools/genomecov/tests/main.nf.test @@ -0,0 +1,118 @@ +nextflow_process { + name "Test Process BEDTOOLS_GENOMECOV" + script "../main.nf" + process "BEDTOOLS_GENOMECOV" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "bedtools" + tag "bedtools/genomecov" + + test("sarscov2 - no scale") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true), + 1 + ] + // sizes + input[1] = [] + // extension + input[2] = "txt" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match("no_scale") } + ) + } + + } + + test("sarscov2 - dummy sizes") { + when { + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true), + 0.5 + ] + // sizes + input[1] = file('dummy_chromosome_sizes') + // extension + input[2] = 'txt' + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match("dummy_sizes") } + ) + } + + } + + test("sarscov2 - scale") { + when { + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['baits_bed'], checkIfExists: true), + 0.5 + ] + // sizes + input[1] = file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + // extension + input[2] = 'txt' + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match("scale") } + ) + } + + } + + test("stub") { + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true), + 1 + ] + // sizes + input[1] = [] + // extension + input[2] = 'txt' + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.genomecov[0][1]).name).match("stub") } + ) + } + + } + +} diff --git a/modules/nf-core/bedtools/genomecov/tests/main.nf.test.snap b/modules/nf-core/bedtools/genomecov/tests/main.nf.test.snap new file mode 100644 index 00000000..8f9191e4 --- /dev/null +++ b/modules/nf-core/bedtools/genomecov/tests/main.nf.test.snap @@ -0,0 +1,95 @@ +{ + "dummy_sizes": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.coverage.txt:md5,01291b6e1beab72e046653e709eb0e10" + ] + ], + "1": [ + "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2" + ], + "genomecov": [ + [ + { + "id": "test" + }, + "test.coverage.txt:md5,01291b6e1beab72e046653e709eb0e10" + ] + ], + "versions": [ + "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2" + ] + } + ], + "timestamp": "2023-12-05T17:35:58.35232" + }, + "no_scale": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.coverage.txt:md5,66083198daca6c001d328ba9616e9b53" + ] + ], + "1": [ + "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2" + ], + "genomecov": [ + [ + { + "id": "test" + }, + "test.coverage.txt:md5,66083198daca6c001d328ba9616e9b53" + ] + ], + "versions": [ + "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2" + ] + } + ], + "timestamp": "2023-12-05T17:35:51.142496" + }, + "stub": { + "content": [ + "test.coverage.txt" + ], + "timestamp": "2023-12-05T17:36:13.084709" + }, + "scale": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.coverage.txt:md5,de3c59c0ea123bcdbbad27bc0a0a601e" + ] + ], + "1": [ + "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2" + ], + "genomecov": [ + [ + { + "id": "test" + }, + "test.coverage.txt:md5,de3c59c0ea123bcdbbad27bc0a0a601e" + ] + ], + "versions": [ + "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2" + ] + } + ], + "timestamp": "2023-12-05T17:36:05.962006" + } +} \ No newline at end of file diff --git a/modules/nf-core/bedtools/genomecov/tests/nextflow.config b/modules/nf-core/bedtools/genomecov/tests/nextflow.config new file mode 100644 index 00000000..bdb74ae5 --- /dev/null +++ b/modules/nf-core/bedtools/genomecov/tests/nextflow.config @@ -0,0 +1,7 @@ +process { + + withName: BEDTOOLS_GENOMECOV { + ext.prefix = { "${meta.id}.coverage" } + } + +} diff --git a/modules/nf-core/bedtools/genomecov/tests/tags.yml b/modules/nf-core/bedtools/genomecov/tests/tags.yml new file mode 100644 index 00000000..55fce478 --- /dev/null +++ b/modules/nf-core/bedtools/genomecov/tests/tags.yml @@ -0,0 +1,2 @@ +bedtools/genomecov: + - "modules/nf-core/bedtools/genomecov/**" From 8c32fe124df1a95c4ce857ad5cac262b4ec16d63 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 30 Jan 2024 01:20:32 +0100 Subject: [PATCH 281/387] add subsample_mt --- conf/modules/subsample_mt.config | 23 +++++++++++++++++++++++ nextflow.config | 3 ++- subworkflows/local/subsample_mt.nf | 22 ++++++++++++++++++++++ workflows/raredisease.nf | 3 +++ 4 files changed, 50 insertions(+), 1 deletion(-) create mode 100644 conf/modules/subsample_mt.config create mode 100644 subworkflows/local/subsample_mt.nf diff --git a/conf/modules/subsample_mt.config b/conf/modules/subsample_mt.config new file mode 100644 index 00000000..079affe9 --- /dev/null +++ b/conf/modules/subsample_mt.config @@ -0,0 +1,23 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. + ext.when = Conditional clause +---------------------------------------------------------------------------------------- +*/ + +// +// Subsample MT +// + +process { + withName: '.*BEDTOOLS_GENOMECOV' { + ext.args = { "-dz" } + ext.prefix = { "${meta.id}" } + } +} diff --git a/nextflow.config b/nextflow.config index 681972ca..c851ac89 100644 --- a/nextflow.config +++ b/nextflow.config @@ -243,7 +243,7 @@ if (!params.igenomes_ignore) { // Load nf-core/raredisease custom config try { - includeConfig "${params.custom_config_base}/pipeline/raredisease.config" + includeConfig "https://raw.githubusercontent.com/nf-core/configs/683bae73d91856a97cd1dbbfe3e3dde4ae5373a1/pipeline/raredisease.config" } catch (Exception e) { System.err.println("WARNING: Could not load nf-core/config/raredisease profiles: ${params.custom_config_base}/pipeline/raredisease.config") } @@ -337,6 +337,7 @@ includeConfig 'conf/modules/call_mobile_elements.config' includeConfig 'conf/modules/annotate_mobile_elements.config' includeConfig 'conf/modules/generate_clinical_set.config' includeConfig 'conf/modules/variant_evaluation.config' +includeConfig 'conf/modules/subsample_mt.config' // Function to ensure that resource requirements don't go beyond // a maximum limit diff --git a/subworkflows/local/subsample_mt.nf b/subworkflows/local/subsample_mt.nf new file mode 100644 index 00000000..6ffe8c98 --- /dev/null +++ b/subworkflows/local/subsample_mt.nf @@ -0,0 +1,22 @@ +// +// A subworkflow to subsample MT alignments +// + +include { BEDTOOLS_GENOMECOV } from '../../modules/nf-core/bedtools/genomecov/main' + +workflow SUBSAMPLE_MT { + + take: + ch_mt_marked_bam // channel: [mandatory] [ val(meta), path(vcf), path(tbi) ] + + main: + + ch_mt_marked_bam.map {meta, bam -> return [meta, bam, []]}.set {ch_genomecov_in} + + BEDTOOLS_GENOMECOV (ch_genomecov_in, [], []) + + ch_versions = ch_versions.mix(BEDTOOLS_GENOMECOV.out.versions.first()) + + emit: + versions = ch_versions // channel: [ path(versions.yml) ] +} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 9f402370..1ee3f1c9 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -140,6 +140,7 @@ include { RANK_VARIANTS as RANK_VARIANTS_MT } from '../subworkf include { RANK_VARIANTS as RANK_VARIANTS_SNV } from '../subworkflows/local/rank_variants' include { RANK_VARIANTS as RANK_VARIANTS_SV } from '../subworkflows/local/rank_variants' include { SCATTER_GENOME } from '../subworkflows/local/scatter_genome' +include { SUBSAMPLE_MT } from '../subworkflows/local/subsample_mt' include { VARIANT_EVALUATION } from '../subworkflows/local/variant_evaluation' /* @@ -362,6 +363,8 @@ workflow RAREDISEASE { .set { ch_mapped } ch_versions = ch_versions.mix(ALIGN.out.versions) + SUBSAMPLE_MT(ch_mapped.mt_marked_bam) + // // BAM QUALITY CHECK // From b434944db334a09f934767e33b9269639bef0518 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 30 Jan 2024 10:58:16 +0100 Subject: [PATCH 282/387] fix few lint errors --- main.nf | 3 --- nextflow.config | 3 +++ nextflow_schema.json | 8 ++++---- 3 files changed, 7 insertions(+), 7 deletions(-) diff --git a/main.nf b/main.nf index ee2294fe..72b342a3 100644 --- a/main.nf +++ b/main.nf @@ -35,7 +35,6 @@ params.known_dbsnp_tbi = WorkflowMain.getGenomeAttribute(params, params.mobile_element_references = WorkflowMain.getGenomeAttribute(params, 'mobile_element_references') params.ml_model = WorkflowMain.getGenomeAttribute(params, 'ml_model') params.mt_fasta = WorkflowMain.getGenomeAttribute(params, 'mt_fasta') -params.ngsbits_samplegender_method = WorkflowMain.getGenomeAttribute(params, 'ngsbits_samplegender_method') params.ploidy_model = WorkflowMain.getGenomeAttribute(params, 'ploidy_model') params.reduced_penetrance = WorkflowMain.getGenomeAttribute(params, 'reduced_penetrance') params.readcount_intervals = WorkflowMain.getGenomeAttribute(params, 'readcount_intervals') @@ -47,7 +46,6 @@ params.score_config_sv = WorkflowMain.getGenomeAttribute(params, params.sdf = WorkflowMain.getGenomeAttribute(params, 'sdf') params.svdb_query_dbs = WorkflowMain.getGenomeAttribute(params, 'svdb_query_dbs') params.target_bed = WorkflowMain.getGenomeAttribute(params, 'target_bed') -params.min_trimmed_length = WorkflowMain.getGenomeAttribute(params, 'min_trimmed_length') params.variant_catalog = WorkflowMain.getGenomeAttribute(params, 'variant_catalog') params.vep_filters = WorkflowMain.getGenomeAttribute(params, 'vep_filters') params.vcf2cytosure_blacklist = WorkflowMain.getGenomeAttribute(params, 'vcf2cytosure_blacklist') @@ -55,7 +53,6 @@ params.vcfanno_resources = WorkflowMain.getGenomeAttribute(params, params.vcfanno_toml = WorkflowMain.getGenomeAttribute(params, 'vcfanno_toml') params.vcfanno_lua = WorkflowMain.getGenomeAttribute(params, 'vcfanno_lua') params.vep_cache = WorkflowMain.getGenomeAttribute(params, 'vep_cache') -params.vep_cache_version = WorkflowMain.getGenomeAttribute(params, 'vep_cache_version') params.vep_plugin_files = WorkflowMain.getGenomeAttribute(params, 'vep_plugin_files') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ diff --git a/nextflow.config b/nextflow.config index 8957a411..7fec01c5 100644 --- a/nextflow.config +++ b/nextflow.config @@ -59,6 +59,9 @@ params { cnvnator_binsize = 1000 variant_caller = 'deepvariant' + // variant annotation + vep_cache_version = 110 + // sentieon Defaults ml_model = '' diff --git a/nextflow_schema.json b/nextflow_schema.json index 04c55dd6..74b35fd8 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -451,9 +451,9 @@ "ngsbits_samplegender_method": { "type": "string", "default": "xy", - "enum": ["xy", "hetx", "sry"], "description": "Method selection for ngs-bits samplegender", - "fa_icon": "fas fa-align-center" + "fa_icon": "fas fa-align-center", + "enum": ["xy", "hetx", "sry"] }, "run_rtgvcfeval": { "type": "boolean", @@ -569,7 +569,7 @@ "description": "Interval in the reference that will be used in the software" }, "cnvnator_binsize": { - "type": "number", + "type": "integer", "description": "Bin size for CNVnator", "default": 1000, "fa_icon": "fas fa-align-center" @@ -591,7 +591,7 @@ }, "variant_type": { "type": "string", - "default": "snp", + "default": "snp,indel", "description": "Specifies the variant types for sentieon variant caller.", "fa_icon": "fas fa-align-center", "enum": ["snp", "indel", "snp,indel"] From d3942a5432b98e1502b7e687da11dd2cde1aa6e7 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Luc=C3=ADa=20Pe=C3=B1a-P=C3=A9rez?= Date: Tue, 30 Jan 2024 11:46:50 +0100 Subject: [PATCH 283/387] Apply suggestions from code review --- conf/modules/annotate_genome_snvs.config | 2 +- conf/modules/annotate_mobile_elements.config | 2 +- conf/modules/annotate_mt_snvs.config | 2 +- conf/modules/annotate_structural_variants.config | 2 +- 4 files changed, 4 insertions(+), 4 deletions(-) diff --git a/conf/modules/annotate_genome_snvs.config b/conf/modules/annotate_genome_snvs.config index c1bb9144..09891e43 100644 --- a/conf/modules/annotate_genome_snvs.config +++ b/conf/modules/annotate_genome_snvs.config @@ -80,7 +80,7 @@ process { ext.args = { [ '--dir_plugins vep_cache/Plugins', '--plugin LoFtool,LoFtool_scores.txt', - "--plugin pLI,pLI_values_${params.vep_cache_version}.txt", + "--plugin pLI,pLI_values.txt", '--plugin SpliceAI,snv=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', '--distance 5000', '--buffer_size 20000', diff --git a/conf/modules/annotate_mobile_elements.config b/conf/modules/annotate_mobile_elements.config index e94f67af..98f8fa15 100644 --- a/conf/modules/annotate_mobile_elements.config +++ b/conf/modules/annotate_mobile_elements.config @@ -40,7 +40,7 @@ process { ext.args = { [ '--dir_cache vep_cache', '--dir_plugins vep_cache/Plugins', - "--plugin pLI,pLI_values_${params.vep_cache_version}.txt", + "--plugin pLI,pLI_values.txt", '--appris --biotype --buffer_size 100 --canonical --cache --ccds', '--compress_output bgzip --distance 5000 --domains', '--exclude_predicted --force_overwrite --format vcf', diff --git a/conf/modules/annotate_mt_snvs.config b/conf/modules/annotate_mt_snvs.config index dfa7ea2f..0ee1c471 100644 --- a/conf/modules/annotate_mt_snvs.config +++ b/conf/modules/annotate_mt_snvs.config @@ -21,7 +21,7 @@ process { ext.args = { [ '--dir_plugins vep_cache/Plugins', '--plugin LoFtool,LoFtool_scores.txt', - "--plugin pLI,pLI_values_${params.vep_cache_version}.txt", + "--plugin pLI,pLI_values.txt", '--plugin SpliceAI,snv=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', '--distance 0', '--buffer_size 20000', diff --git a/conf/modules/annotate_structural_variants.config b/conf/modules/annotate_structural_variants.config index 74425f31..89f88b30 100644 --- a/conf/modules/annotate_structural_variants.config +++ b/conf/modules/annotate_structural_variants.config @@ -46,7 +46,7 @@ process { ext.args = { [ '--dir_cache vep_cache', '--dir_plugins vep_cache/Plugins', - "--plugin pLI,pLI_values_${params.vep_cache_version}.txt", + "--plugin pLI,pLI_values.txt", '--appris --biotype --buffer_size 100 --canonical --cache --ccds', '--compress_output bgzip --distance 5000 --domains', '--exclude_predicted --force_overwrite --format vcf', From 24afea99b7056bd26f5c970ec27b39fbab9130bf Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Luc=C3=ADa=20Pe=C3=B1a-P=C3=A9rez?= Date: Tue, 30 Jan 2024 11:48:19 +0100 Subject: [PATCH 284/387] Apply suggestions from code review --- conf/modules/annotate_genome_snvs.config | 2 +- conf/modules/annotate_mobile_elements.config | 2 +- conf/modules/annotate_mt_snvs.config | 2 +- conf/modules/annotate_structural_variants.config | 2 +- 4 files changed, 4 insertions(+), 4 deletions(-) diff --git a/conf/modules/annotate_genome_snvs.config b/conf/modules/annotate_genome_snvs.config index 09891e43..bf748350 100644 --- a/conf/modules/annotate_genome_snvs.config +++ b/conf/modules/annotate_genome_snvs.config @@ -80,7 +80,7 @@ process { ext.args = { [ '--dir_plugins vep_cache/Plugins', '--plugin LoFtool,LoFtool_scores.txt', - "--plugin pLI,pLI_values.txt", + '--plugin pLI,pLI_values.txt', '--plugin SpliceAI,snv=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', '--distance 5000', '--buffer_size 20000', diff --git a/conf/modules/annotate_mobile_elements.config b/conf/modules/annotate_mobile_elements.config index 98f8fa15..dec67a22 100644 --- a/conf/modules/annotate_mobile_elements.config +++ b/conf/modules/annotate_mobile_elements.config @@ -40,7 +40,7 @@ process { ext.args = { [ '--dir_cache vep_cache', '--dir_plugins vep_cache/Plugins', - "--plugin pLI,pLI_values.txt", + '--plugin pLI,pLI_values.txt', '--appris --biotype --buffer_size 100 --canonical --cache --ccds', '--compress_output bgzip --distance 5000 --domains', '--exclude_predicted --force_overwrite --format vcf', diff --git a/conf/modules/annotate_mt_snvs.config b/conf/modules/annotate_mt_snvs.config index 0ee1c471..45260fb2 100644 --- a/conf/modules/annotate_mt_snvs.config +++ b/conf/modules/annotate_mt_snvs.config @@ -21,7 +21,7 @@ process { ext.args = { [ '--dir_plugins vep_cache/Plugins', '--plugin LoFtool,LoFtool_scores.txt', - "--plugin pLI,pLI_values.txt", + '--plugin pLI,pLI_values.txt', '--plugin SpliceAI,snv=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', '--distance 0', '--buffer_size 20000', diff --git a/conf/modules/annotate_structural_variants.config b/conf/modules/annotate_structural_variants.config index 89f88b30..62faeda7 100644 --- a/conf/modules/annotate_structural_variants.config +++ b/conf/modules/annotate_structural_variants.config @@ -46,7 +46,7 @@ process { ext.args = { [ '--dir_cache vep_cache', '--dir_plugins vep_cache/Plugins', - "--plugin pLI,pLI_values.txt", + '--plugin pLI,pLI_values.txt', '--appris --biotype --buffer_size 100 --canonical --cache --ccds', '--compress_output bgzip --distance 5000 --domains', '--exclude_predicted --force_overwrite --format vcf', From 0a44420eeac1dc8d529dcae0e525f10da0ac7ee9 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 30 Jan 2024 13:23:42 +0100 Subject: [PATCH 285/387] fix pre-commit --- .devcontainer/devcontainer.json | 8 +- .editorconfig | 4 +- .github/workflows/download_pipeline.yml | 2 +- assets/svrank_model.ini | 443 ------------------ subworkflows/local/call_repeat_expansions.nf | 4 +- .../local/variant_calling/call_sv_MT.nf | 2 +- workflows/raredisease.nf | 126 ++--- 7 files changed, 73 insertions(+), 516 deletions(-) delete mode 100644 assets/svrank_model.ini diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4a9bc5c7..4ecfbfe3 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,11 +18,11 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint", + "python.linting.pylintPath": "/opt/conda/bin/pylint" }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], - }, - }, + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] + } + } } diff --git a/.editorconfig b/.editorconfig index 9b990088..8c4ce4d0 100644 --- a/.editorconfig +++ b/.editorconfig @@ -27,6 +27,6 @@ indent_size = unset [README.md] indent_style = unset -# ignore python -[*.{py}] +# ignore python and markdown +[*.{py,md}] indent_style = unset diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458a..8a330045 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -64,4 +64,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/assets/svrank_model.ini b/assets/svrank_model.ini deleted file mode 100644 index 510aadf1..00000000 --- a/assets/svrank_model.ini +++ /dev/null @@ -1,443 +0,0 @@ -[Version] - version = 1.8 - name = svrank_model - -[Categories] - [[allele_frequency]] - category_aggregation = min - - [[clinical_significance]] - category_aggregation = sum - - [[consequence]] - category_aggregation = max - - [[gene_intolerance_prediction]] - category_aggregation = sum - - [[inheritance_models]] - category_aggregation = min - - [[variant_call_quality_filter]] - category_aggregate = sum - - [[variant_length]] - category_aggregation = min - - [[variant_type]] - category_aggregation = min - -[gnomad_sv] - category = allele_frequency - data_type = float - description = Gnomad sv genomes frequency - field = INFO - info_key = gnomad_svAF - record_rule = max - separators = ',', - - [[not_reported]] - score = 6 - - [[common]] - score = -12 - lower = 0.02 - upper = 1.1 - - [[intermediate]] - score = 1 - lower = 0.005 - upper = 0.02 - - [[rare]] - score = 2 - lower = 0.0005 - upper = 0.005 - - [[very_rare]] - score = 4 - lower = 0 - upper = 0.0005 - -[swegen] - category = allele_frequency - data_type = float - description = Swegen genomes frequency - field = INFO - info_key = swegenAF - record_rule = max - separators = ',', - - [[not_reported]] - score = 6 - - [[common]] - score = -12 - lower = 0.02 - upper = 1.1 - - [[intermediate]] - score = 1 - lower = 0.005 - upper = 0.02 - - [[rare]] - score = 2 - lower = 0.0005 - upper = 0.005 - - [[very_rare]] - score = 4 - lower = 0 - upper = 0.0005 - -[sv_len] - category = variant_length - data_type = integer - description = The length of the structural variant - field = INFO - info_key = SVLEN - record_rule = min - separators = ',', - - [[not_reported]] - score = 0 - - [[long_pos]] - score = 3 - lower = 1000001 - upper = 100000000 - - [[long_neg]] - score = 3 - lower = -100000000 - upper = -1000001 - - [[medium_pos]] - score = 3 - lower = 50001 - upper = 1000000 - - [[medium_neg]] - score = 3 - lower = -1000000 - upper = -50001 - -[gene_intolerance_score] - category = gene_intolerance_prediction - data_type = float - description = Gnomad gene intolerance prediction - field = INFO - info_key = most_severe_pli - record_rule = max - separators = None - - [[not_reported]] - score = 0 - - [[low_intolerance]] - score = 0 - lower = 0 - upper = 0.90 - - [[medium_intolerance]] - score = 2 - lower = 0.90 - upper = 0.99 - - [[high_intolerance]] - score = 4 - lower = 0.99 - upper = 1.1 - -[genetic_models] - data_type = string - description = The inheritance models followed for the variant - category = inheritance_models - field = INFO - info_key = GeneticModels - record_rule = max - separators = ',', ':', '|', - - [[ad]] - priority = 1 - score = 3 - string = 'AD' - - [[ad_dn]] - priority = 1 - score = 3 - string = 'AD_dn' - - [[ar]] - priority = 1 - score = 3 - string = 'AR_hom' - - [[ar_dn]] - priority = 1 - score = 3 - string = 'AR_hom_dn' - - [[ar_comp]] - priority = 1 - score = 3 - string = 'AR_comp' - - [[ar_comp_dn]] - priority = 1 - score = 3 - string = 'AR_comp_dn' - - [[xr]] - priority = 1 - score = 3 - string = 'XR' - - [[xr_dn]] - priority = 1 - score = 3 - string = 'XR_dn' - - [[xd]] - priority = 1 - score = 3 - string = 'XD' - - [[xd_dn]] - priority = 1 - score = 3 - string = 'XD_dn' - - [[not_reported]] - score = 0 - -[model_score] - category = variant_call_quality_filter - data_type = integer - description = The inheritance model score - field = INFO - info_key = ModelScore - record_rule = min - separators = ',',':', - - [[not_reported]] - score = 0 - - [[low_qual]] - score = -5 - lower = 0 - upper = 20 - - [[high_qual]] - score = 0 - lower = 20 - upper = 300 - -[most_severe_consequence] - category = consequence - data_type = string - description = The most severe consequence for this variant - field = INFO - info_key = most_severe_consequence - record_rule = max - separators = ',', ':', '|', - - [[transcript_ablation]] - score = 10 - priority = 6 - string = 'transcript_ablation' - - [[initiator_codon_variant]] - score = 9 - priority = 5 - string = 'initiator_codon_variant' - - [[frameshift_variant]] - score = 8 - priority = 5 - string = 'frameshift_variant' - - [[stop_gained]] - score = 8 - priority = 5 - string = 'stop_gained' - - [[start_lost]] - score = 8 - priority = 5 - string = 'start_lost' - - [[stop_lost]] - score = 8 - priority = 5 - string = 'stop_lost' - - [[splice_acceptor_variant]] - score = 8 - priority = 5 - string = 'splice_acceptor_variant' - - [[splice_donor_variant]] - score = 8 - priority = 5 - string = 'splice_donor_variant' - - [[coding_sequence_variant]] - score = 7 - priority = 4 - string = 'coding_sequence_variant' - - [[inframe_deletion]] - score = 5 - priority = 4 - string = 'inframe_deletion' - - [[transcript_amplification]] - score = 5 - priority = 4 - string = 'transcript_amplification' - - [[splice_region_variant]] - score = 5 - priority = 4 - string = 'splice_region_variant' - - [[missense_variant]] - score = 5 - priority = 4 - string = 'missense_variant' - - [[protein_altering_variant]] - score = 5 - priority = 4 - string = 'protein_altering_variant' - - [[inframe_insertion]] - score = 5 - priority = 4 - string = 'inframe_insertion' - - [[incomplete_terminal_codon_variant]] - score = 5 - priority = 4 - string = 'incomplete_terminal_codon_variant' - - [[non_coding_transcript_exon_variant]] - score = 3 - priority = 2 - string = 'non_coding_transcript_exon_variant' - - [[synonymous_variant]] - score = 2 - priority = 2 - string = 'synonymous_variant' - - [[mature_mirna_variant]] - score = 1 - priority = 2 - string = 'mature_mirna_variant' - - [[non_coding_transcript_variant]] - score = 1 - priority = 2 - string = 'non_coding_transcript_variant' - - [[regulatory_region_variant]] - score = 1 - priority = 2 - string = 'regulatory_region_variant' - - [[upstream_gene_variant]] - score = 1 - priority = 2 - string = 'upstream_gene_variant' - - [[regulatory_region_amplification]] - score = 1 - priority = 2 - string = 'regulatory_region_amplification' - - [[tfbs_amplification]] - score = 1 - priority = 2 - string = 'tfbs_amplification' - - [[5_prime_utr_variant]] - score = 1 - priority = 2 - string = '5_prime_utr_variant' - - [[intron_variant]] - score = 1 - priority = 2 - string = 'intron_variant' - - [[3_prime_utr_variant]] - score = 1 - priority = 2 - string = '3_prime_utr_variant' - - [[feature_truncation]] - score = 1 - priority = 2 - string = 'feature_truncation' - - [[TF_binding_site_variant]] - score = 1 - priority = 2 - string = 'TF_binding_site_variant' - - [[stop_retained_variant]] - score = 1 - priority = 2 - string = 'stop_retained_variant' - - [[feature_elongation]] - score = 1 - priority = 2 - string = 'feature_elongation' - - [[regulatory_region_ablation]] - score = 1 - priority = 2 - string = 'regulatory_region_ablation' - - [[tfbs_ablation]] - score = 1 - priority = 2 - string = 'tfbs_ablation' - - [[downstream_gene_variant]] - score = 1 - priority = 2 - string = 'downstream_gene_variant' - - [[NMD_transcript_variant]] - score = 1 - priority = 2 - string = 'NMD_transcript_variant' - - [[intergenic_variant]] - score = 0 - priority = 0 - string = 'intergenic_variant' - - [[not_reported]] - score = 0 - -[filter] - category = variant_call_quality_filter - data_type = string - description = The filters for the variant - field = FILTER - record_rule = min - separators = ';', - - [[not_reported]] - score = 0 - - [[pass]] - score = 3 - priority = 1 - string = 'PASS' diff --git a/subworkflows/local/call_repeat_expansions.nf b/subworkflows/local/call_repeat_expansions.nf index 1fd76cb9..9d86072c 100644 --- a/subworkflows/local/call_repeat_expansions.nf +++ b/subworkflows/local/call_repeat_expansions.nf @@ -62,7 +62,7 @@ workflow CALL_REPEAT_EXPANSIONS { STRANGER ( SVDB_MERGE_REPEATS.out.vcf, ch_variant_catalog ) COMPRESS_STRANGER ( STRANGER.out.vcf.map{ meta, vcf -> [meta, vcf, [] ]}, - [], [], [] + [], [], [] ) INDEX_STRANGER ( COMPRESS_STRANGER.out.vcf ) @@ -78,7 +78,7 @@ workflow CALL_REPEAT_EXPANSIONS { ch_versions = ch_versions.mix(COMPRESS_STRANGER.out.versions.first()) ch_versions = ch_versions.mix(INDEX_STRANGER.out.versions.first()) - emit: +emit: vcf = ch_vcf_idx // channel: [ val(meta), path(vcf), path(tbi) ] versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/subworkflows/local/variant_calling/call_sv_MT.nf b/subworkflows/local/variant_calling/call_sv_MT.nf index 7d6f4ede..7ce39b42 100644 --- a/subworkflows/local/variant_calling/call_sv_MT.nf +++ b/subworkflows/local/variant_calling/call_sv_MT.nf @@ -22,7 +22,7 @@ workflow CALL_SV_MT { ch_eklipse_genes = EKLIPSE.out.genes ch_eklipse_circos = EKLIPSE.out.circos ch_versions = ch_versions.mix(EKLIPSE.out.versions.first()) - } + } MT_DELETION(ch_bam_bai, ch_fasta) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 09d38c54..f01871ce 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -221,40 +221,40 @@ workflow RAREDISEASE { // Gather built indices or get them from the params ch_bait_intervals = ch_references.bait_intervals ch_cadd_header = Channel.fromPath("$projectDir/assets/cadd_to_vcf_header_-1.0-.txt", checkIfExists: true).collect() - ch_cadd_resources = params.cadd_resources ? Channel.fromPath(params.cadd_resources).collect() - : Channel.value([]) - ch_call_interval = params.call_interval ? Channel.fromPath(params.call_interval).map {it -> [[id:it[0].simpleName], it]}.collect() - : Channel.value([[:],[]]) - ch_dbsnp_tbi = params.known_dbsnp_tbi ? Channel.fromPath(params.known_dbsnp_tbi).map {it -> [[id:it[0].simpleName], it]}.collect() - : ch_references.known_dbsnp_tbi.ifEmpty([[],[]]) + ch_cadd_resources = params.cadd_resources ? Channel.fromPath(params.cadd_resources).collect() + : Channel.value([]) + ch_call_interval = params.call_interval ? Channel.fromPath(params.call_interval).map {it -> [[id:it[0].simpleName], it]}.collect() + : Channel.value([[:],[]]) + ch_dbsnp_tbi = params.known_dbsnp_tbi ? Channel.fromPath(params.known_dbsnp_tbi).map {it -> [[id:it[0].simpleName], it]}.collect() + : ch_references.known_dbsnp_tbi.ifEmpty([[],[]]) ch_foundin_header = Channel.fromPath("$projectDir/assets/foundin.hdr", checkIfExists: true).collect() - ch_gcnvcaller_model = params.gcnvcaller_model ? Channel.fromPath(params.gcnvcaller_model).splitCsv ( header:true ) + ch_gcnvcaller_model = params.gcnvcaller_model ? Channel.fromPath(params.gcnvcaller_model).splitCsv ( header:true ) .map { row -> return [[id:file(row.models).simpleName], row.models] } - : Channel.empty() - ch_genome_bwaindex = params.bwa ? Channel.fromPath(params.bwa).map {it -> [[id:it[0].simpleName], it]}.collect() - : ch_references.genome_bwa_index - ch_genome_bwamem2index = params.bwamem2 ? Channel.fromPath(params.bwamem2).map {it -> [[id:it[0].simpleName], it]}.collect() - : ch_references.genome_bwamem2_index + : Channel.empty() + ch_genome_bwaindex = params.bwa ? Channel.fromPath(params.bwa).map {it -> [[id:it[0].simpleName], it]}.collect() + : ch_references.genome_bwa_index + ch_genome_bwamem2index = params.bwamem2 ? Channel.fromPath(params.bwamem2).map {it -> [[id:it[0].simpleName], it]}.collect() + : ch_references.genome_bwamem2_index ch_genome_chrsizes = ch_references.genome_chrom_sizes ch_genome_fai = ch_references.genome_fai - ch_genome_dictionary = params.sequence_dictionary ? Channel.fromPath(params.sequence_dictionary).map {it -> [[id:it[0].simpleName], it]}.collect() - : ch_references.genome_dict - ch_gnomad_afidx = params.gnomad_af_idx ? Channel.fromPath(params.gnomad_af_idx).collect() - : ch_references.gnomad_af_idx - ch_gnomad_af = params.gnomad_af ? ch_gnomad_af_tab.join(ch_gnomad_afidx).map {meta, tab, idx -> [tab,idx]}.collect() - : Channel.empty() - ch_intervals_wgs = params.intervals_wgs ? Channel.fromPath(params.intervals_wgs).collect() - : Channel.empty() - ch_intervals_y = params.intervals_y ? Channel.fromPath(params.intervals_y).collect() - : Channel.empty() - ch_me_references = params.mobile_element_references ? Channel.fromSamplesheet("mobile_element_references") - : Channel.empty() - ch_me_svdb_resources = params.mobile_element_svdb_annotations ? Channel.fromPath(params.mobile_element_svdb_annotations) - : Channel.empty() - ch_ml_model = params.variant_caller.equals("sentieon") ? Channel.fromPath(params.ml_model).map {it -> [[id:it[0].simpleName], it]}.collect() - : Channel.value([[:],[]]) + ch_genome_dictionary = params.sequence_dictionary ? Channel.fromPath(params.sequence_dictionary).map {it -> [[id:it[0].simpleName], it]}.collect() + : ch_references.genome_dict + ch_gnomad_afidx = params.gnomad_af_idx ? Channel.fromPath(params.gnomad_af_idx).collect() + : ch_references.gnomad_af_idx + ch_gnomad_af = params.gnomad_af ? ch_gnomad_af_tab.join(ch_gnomad_afidx).map {meta, tab, idx -> [tab,idx]}.collect() + : Channel.empty() + ch_intervals_wgs = params.intervals_wgs ? Channel.fromPath(params.intervals_wgs).collect() + : Channel.empty() + ch_intervals_y = params.intervals_y ? Channel.fromPath(params.intervals_y).collect() + : Channel.empty() + ch_me_references = params.mobile_element_references ? Channel.fromSamplesheet("mobile_element_references") + : Channel.empty() + ch_me_svdb_resources = params.mobile_element_svdb_annotations ? Channel.fromPath(params.mobile_element_svdb_annotations) + : Channel.empty() + ch_ml_model = params.variant_caller.equals("sentieon") ? Channel.fromPath(params.ml_model).map {it -> [[id:it[0].simpleName], it]}.collect() + : Channel.value([[:],[]]) ch_mt_intervals = ch_references.mt_intervals ch_mtshift_backchain = ch_references.mtshift_backchain ch_mtshift_bwaindex = ch_references.mtshift_bwa_index @@ -263,45 +263,45 @@ workflow RAREDISEASE { ch_mtshift_fai = ch_references.mtshift_fai ch_mtshift_fasta = ch_references.mtshift_fasta ch_mtshift_intervals = ch_references.mtshift_intervals - ch_ploidy_model = params.ploidy_model ? Channel.fromPath(params.ploidy_model).map{ it -> [[id:it[0].simpleName], it] }.collect() - : Channel.empty() - ch_readcount_intervals = params.readcount_intervals ? Channel.fromPath(params.readcount_intervals).collect() - : ( ch_references.readcount_intervals ?: Channel.empty() ) - ch_reduced_penetrance = params.reduced_penetrance ? Channel.fromPath(params.reduced_penetrance).collect() - : Channel.value([]) - ch_rtg_truthvcfs = params.rtg_truthvcfs ? Channel.fromPath(params.rtg_truthvcfs).collect() - : Channel.value([]) - ch_score_config_mt = params.score_config_mt ? Channel.fromPath(params.score_config_mt).collect() - : Channel.value([]) - ch_score_config_snv = params.score_config_snv ? Channel.fromPath(params.score_config_snv).collect() - : Channel.value([]) - ch_score_config_sv = params.score_config_sv ? Channel.fromPath(params.score_config_sv).collect() - : Channel.value([]) - ch_sdf = params.sdf ? Channel.fromPath(params.sdf).map{it -> [[id:it[0].simpleName],it]}.collect() - : ch_references.sdf - ch_sv_dbs = params.svdb_query_dbs ? Channel.fromPath(params.svdb_query_dbs) - : Channel.empty() - ch_sv_bedpedbs = params.svdb_query_bedpedbs ? Channel.fromPath(params.svdb_query_bedpedbs) - : Channel.empty() + ch_ploidy_model = params.ploidy_model ? Channel.fromPath(params.ploidy_model).map{ it -> [[id:it[0].simpleName], it] }.collect() + : Channel.empty() + ch_readcount_intervals = params.readcount_intervals ? Channel.fromPath(params.readcount_intervals).collect() + : ( ch_references.readcount_intervals ?: Channel.empty() ) + ch_reduced_penetrance = params.reduced_penetrance ? Channel.fromPath(params.reduced_penetrance).collect() + : Channel.value([]) + ch_rtg_truthvcfs = params.rtg_truthvcfs ? Channel.fromPath(params.rtg_truthvcfs).collect() + : Channel.value([]) + ch_score_config_mt = params.score_config_mt ? Channel.fromPath(params.score_config_mt).collect() + : Channel.value([]) + ch_score_config_snv = params.score_config_snv ? Channel.fromPath(params.score_config_snv).collect() + : Channel.value([]) + ch_score_config_sv = params.score_config_sv ? Channel.fromPath(params.score_config_sv).collect() + : Channel.value([]) + ch_sdf = params.sdf ? Channel.fromPath(params.sdf).map{it -> [[id:it[0].simpleName],it]}.collect() + : ch_references.sdf + ch_sv_dbs = params.svdb_query_dbs ? Channel.fromPath(params.svdb_query_dbs) + : Channel.empty() + ch_sv_bedpedbs = params.svdb_query_bedpedbs ? Channel.fromPath(params.svdb_query_bedpedbs) + : Channel.empty() ch_target_bed = ch_references.target_bed ch_target_intervals = ch_references.target_intervals - ch_variant_catalog = params.variant_catalog ? Channel.fromPath(params.variant_catalog).map { it -> [[id:it[0].simpleName],it]}.collect() - : Channel.value([[],[]]) + ch_variant_catalog = params.variant_catalog ? Channel.fromPath(params.variant_catalog).map { it -> [[id:it[0].simpleName],it]}.collect() + : Channel.value([[],[]]) ch_variant_consequences = Channel.fromPath("$projectDir/assets/variant_consequences_v2.txt", checkIfExists: true).collect() - ch_vcfanno_resources = params.vcfanno_resources ? Channel.fromPath(params.vcfanno_resources).splitText().map{it -> it.trim()}.collect() - : Channel.value([]) - ch_vcf2cytosure_blacklist = params.vcf2cytosure_blacklist ? Channel.fromPath(params.vcf2cytosure_blacklist).collect() - : Channel.value([]) - ch_vcfanno_lua = params.vcfanno_lua ? Channel.fromPath(params.vcfanno_lua).collect() - : Channel.value([]) - ch_vcfanno_toml = params.vcfanno_toml ? Channel.fromPath(params.vcfanno_toml).collect() - : Channel.value([]) + ch_vcfanno_resources = params.vcfanno_resources ? Channel.fromPath(params.vcfanno_resources).splitText().map{it -> it.trim()}.collect() + : Channel.value([]) + ch_vcf2cytosure_blacklist = params.vcf2cytosure_blacklist ? Channel.fromPath(params.vcf2cytosure_blacklist).collect() + : Channel.value([]) + ch_vcfanno_lua = params.vcfanno_lua ? Channel.fromPath(params.vcfanno_lua).collect() + : Channel.value([]) + ch_vcfanno_toml = params.vcfanno_toml ? Channel.fromPath(params.vcfanno_toml).collect() + : Channel.value([]) ch_vep_cache = ( params.vep_cache && params.vep_cache.endsWith("tar.gz") ) ? ch_references.vep_resources - : ( params.vep_cache ? Channel.fromPath(params.vep_cache).collect() : Channel.value([]) ) - ch_vep_extra_files_unsplit = params.vep_plugin_files ? Channel.fromPath(params.vep_plugin_files).collect() - : Channel.value([]) - ch_vep_filters = params.vep_filters ? Channel.fromPath(params.vep_filters).collect() - : Channel.value([]) + : ( params.vep_cache ? Channel.fromPath(params.vep_cache).collect() : Channel.value([]) ) + ch_vep_extra_files_unsplit = params.vep_plugin_files ? Channel.fromPath(params.vep_plugin_files).collect() + : Channel.value([]) + ch_vep_filters = params.vep_filters ? Channel.fromPath(params.vep_filters).collect() + : Channel.value([]) ch_versions = ch_versions.mix(ch_references.versions) // SV caller priority From bbc94c0bdb39b4982abd2e9eca2746f107d09831 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 30 Jan 2024 18:10:29 +0100 Subject: [PATCH 286/387] update expansionhunter --- conf/modules/call_repeat_expansions.config | 17 +++++++++ modules.json | 2 +- modules/nf-core/expansionhunter/main.nf | 8 ++-- modules/nf-core/expansionhunter/meta.yml | 10 ++++- .../expansionhunter/tests/main.nf.test | 37 +++++++++++++++++++ .../expansionhunter/tests/main.nf.test.snap | 15 ++++++++ .../nf-core/expansionhunter/tests/tags.yml | 2 + subworkflows/local/call_repeat_expansions.nf | 8 ++++ 8 files changed, 94 insertions(+), 5 deletions(-) create mode 100644 modules/nf-core/expansionhunter/tests/main.nf.test create mode 100644 modules/nf-core/expansionhunter/tests/main.nf.test.snap create mode 100644 modules/nf-core/expansionhunter/tests/tags.yml diff --git a/conf/modules/call_repeat_expansions.config b/conf/modules/call_repeat_expansions.config index 13a7e519..e31911a7 100644 --- a/conf/modules/call_repeat_expansions.config +++ b/conf/modules/call_repeat_expansions.config @@ -28,6 +28,23 @@ process { ext.prefix = { "${meta.id}_exphunter" } } + withName: '.*CALL_REPEAT_EXPANSIONS:SAMTOOLS_SORT' { + ext.prefix = { "${meta.id}_exphunter_sorted" } + publishDir = [ + path: { "${params.outdir}/repeat_expansions" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + ] + } + + withName: '.*CALL_REPEAT_EXPANSIONS:SAMTOOLS_INDEX' { + publishDir = [ + path: { "${params.outdir}/repeat_expansions" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + ] + } + withName: '.*CALL_REPEAT_EXPANSIONS:BCFTOOLS_REHEADER_EXP' { ext.prefix = { "${meta.id}_reheader" } } diff --git a/modules.json b/modules.json index e33ea340..3ea648f7 100644 --- a/modules.json +++ b/modules.json @@ -122,7 +122,7 @@ }, "expansionhunter": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "3c4b0007393248aa7419a9ec3d14f391cd702f48", "installed_by": ["modules"] }, "fastp": { diff --git a/modules/nf-core/expansionhunter/main.nf b/modules/nf-core/expansionhunter/main.nf index 008b13ad..bea5916a 100644 --- a/modules/nf-core/expansionhunter/main.nf +++ b/modules/nf-core/expansionhunter/main.nf @@ -14,9 +14,10 @@ process EXPANSIONHUNTER { tuple val(meta4), path(variant_catalog) output: - tuple val(meta), path("*.vcf.gz") , emit: vcf - tuple val(meta), path("*.json.gz") , emit: json - path "versions.yml" , emit: versions + tuple val(meta), path("*.vcf.gz") , emit: vcf + tuple val(meta), path("*.json.gz") , emit: json + tuple val(meta), path("*_realigned.bam") , emit: bam + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -49,6 +50,7 @@ process EXPANSIONHUNTER { """ touch ${prefix}.vcf.gz touch ${prefix}.json.gz + touch ${prefix}_realigned.bam cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/expansionhunter/meta.yml b/modules/nf-core/expansionhunter/meta.yml index b970ced4..698529dc 100644 --- a/modules/nf-core/expansionhunter/meta.yml +++ b/modules/nf-core/expansionhunter/meta.yml @@ -24,6 +24,10 @@ input: type: file description: BAM/CRAM file pattern: "*.{bam,cram}" + - bai: + type: file + description: Index of BAM/CRAM file + pattern: "*.{bai,crai}" - meta2: type: map description: | @@ -38,7 +42,7 @@ input: description: | Groovy Map containing reference information e.g. [ id:'test' ] - - fasta: + - fasta_fai: type: file description: Reference genome index pattern: "*.fai" @@ -61,6 +65,10 @@ output: type: file description: File containing software versions pattern: "versions.yml" + - bam: + type: file + description: BAM/CRAM file + pattern: "*.{bam,cram}" - vcf: type: file description: VCF with repeat expansions diff --git a/modules/nf-core/expansionhunter/tests/main.nf.test b/modules/nf-core/expansionhunter/tests/main.nf.test new file mode 100644 index 00000000..6204460a --- /dev/null +++ b/modules/nf-core/expansionhunter/tests/main.nf.test @@ -0,0 +1,37 @@ +nextflow_process { + + name "Test Process EXPANSIONHUNTER" + script "modules/nf-core/expansionhunter/main.nf" + process "EXPANSIONHUNTER" + tag "modules" + tag "modules_nfcore" + tag "expansionhunter" + + test("expansionhunter") { + + when { + process { + """ + input[0] = [ [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true), + ] + input[1] = [[id:'fasta'],file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] + input[2] = [[id:'fasta_fai'],file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true)] + input[3] = [[id:'catalogue'],file(params.test_data['homo_sapiens']['genome']['repeat_expansions'], checkIfExists: true)] + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert path(process.out.vcf.get(0).get(1)).linesGzip.size() == 8}, + {assert path(process.out.json.get(0).get(1)).linesGzip.size() == 27}, + {assert snapshot(process.out.bam).match()} + ) + } + + } + +} diff --git a/modules/nf-core/expansionhunter/tests/main.nf.test.snap b/modules/nf-core/expansionhunter/tests/main.nf.test.snap new file mode 100644 index 00000000..a98fb599 --- /dev/null +++ b/modules/nf-core/expansionhunter/tests/main.nf.test.snap @@ -0,0 +1,15 @@ +{ + "expansionhunter": { + "content": [ + [ + [ + { + "id": "test" + }, + "test_realigned.bam:md5,b37a72c0b97b45e63636a9758f3144d7" + ] + ] + ], + "timestamp": "2024-01-26T17:35:10.45442497" + } +} \ No newline at end of file diff --git a/modules/nf-core/expansionhunter/tests/tags.yml b/modules/nf-core/expansionhunter/tests/tags.yml new file mode 100644 index 00000000..03266351 --- /dev/null +++ b/modules/nf-core/expansionhunter/tests/tags.yml @@ -0,0 +1,2 @@ +expansionhunter: + - "modules/nf-core/expansionhunter/**" diff --git a/subworkflows/local/call_repeat_expansions.nf b/subworkflows/local/call_repeat_expansions.nf index 1fd76cb9..fda99625 100644 --- a/subworkflows/local/call_repeat_expansions.nf +++ b/subworkflows/local/call_repeat_expansions.nf @@ -8,6 +8,8 @@ include { BCFTOOLS_VIEW as COMPRESS_STRANGER } from '../../modules/nf- include { EXPANSIONHUNTER } from '../../modules/nf-core/expansionhunter/main' include { PICARD_RENAMESAMPLEINVCF as RENAMESAMPLE_EXP } from '../../modules/nf-core/picard/renamesampleinvcf/main' include { STRANGER } from '../../modules/nf-core/stranger/main' +include { SAMTOOLS_SORT } from '../../modules/nf-core/samtools/sort/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' include { SVDB_MERGE as SVDB_MERGE_REPEATS } from '../../modules/nf-core/svdb/merge/main' include { TABIX_BGZIPTABIX as BGZIPTABIX_EXP } from '../../modules/nf-core/tabix/bgziptabix/main' include { TABIX_TABIX as INDEX_STRANGER } from '../../modules/nf-core/tabix/tabix/main' @@ -31,6 +33,10 @@ workflow CALL_REPEAT_EXPANSIONS { ch_variant_catalog ) + // Sort and index realigned bam + SAMTOOLS_SORT(EXPANSIONHUNTER.out.bam) + SAMTOOLS_INDEX(SAMTOOLS_SORT.out.bam) + // Fix header and rename sample BCFTOOLS_REHEADER_EXP ( EXPANSIONHUNTER.out.vcf.map{ meta, vcf -> [ meta, vcf, [], [] ]}, @@ -77,6 +83,8 @@ workflow CALL_REPEAT_EXPANSIONS { ch_versions = ch_versions.mix(STRANGER.out.versions.first()) ch_versions = ch_versions.mix(COMPRESS_STRANGER.out.versions.first()) ch_versions = ch_versions.mix(INDEX_STRANGER.out.versions.first()) + ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions.first()) + ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) emit: vcf = ch_vcf_idx // channel: [ val(meta), path(vcf), path(tbi) ] From ea5d37284995e25dbf19f691635e5c6099b46ecf Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 31 Jan 2024 09:56:08 +0100 Subject: [PATCH 287/387] cnvnator patch --- modules.json | 3 +- .../cnvnator/cnvnator/cnvnator-cnvnator.diff | 49 +++++++++++++++++++ modules/nf-core/cnvnator/cnvnator/main.nf | 13 +++-- .../local/variant_calling/call_sv_cnvnator.nf | 10 ++-- 4 files changed, 66 insertions(+), 9 deletions(-) create mode 100644 modules/nf-core/cnvnator/cnvnator/cnvnator-cnvnator.diff diff --git a/modules.json b/modules.json index e33ea340..f7eae8be 100644 --- a/modules.json +++ b/modules.json @@ -88,7 +88,8 @@ "cnvnator/cnvnator": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/cnvnator/cnvnator/cnvnator-cnvnator.diff" }, "cnvnator/convert2vcf": { "branch": "master", diff --git a/modules/nf-core/cnvnator/cnvnator/cnvnator-cnvnator.diff b/modules/nf-core/cnvnator/cnvnator/cnvnator-cnvnator.diff new file mode 100644 index 00000000..8186aa08 --- /dev/null +++ b/modules/nf-core/cnvnator/cnvnator/cnvnator-cnvnator.diff @@ -0,0 +1,49 @@ +Changes in module 'nf-core/cnvnator/cnvnator' +--- modules/nf-core/cnvnator/cnvnator/main.nf ++++ modules/nf-core/cnvnator/cnvnator/main.nf +@@ -1,6 +1,7 @@ + process CNVNATOR_CNVNATOR { + tag "$meta.id" + label 'process_single' ++ stageInMode 'copy' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? +@@ -12,11 +13,12 @@ + tuple val(meta2), path(root) + tuple val(meta3), path(fasta) + tuple val(meta4), path(fai) ++ val step + + output: +- tuple val(output_meta), path("${prefix}.root"), emit: root +- tuple val(output_meta), path("${prefix}.tab") , emit: tab, optional: true +- path "versions.yml" , emit: versions ++ tuple val(output_meta), path("${output_meta.id}_${step}.root"), emit: root ++ tuple val(output_meta), path("*.tab") , emit: tab, optional: true ++ path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when +@@ -32,7 +34,11 @@ + reference = '' + } + calls_cmd = args.contains("-call") ? "> ${prefix}.tab" : '' ++ mv_cmd = "mv ${prefix}.root ${prefix}_${step}.root" ++ steps = ["his", "stat", "partition", "call"] ++ cp_cmd = steps.contains(step) ? "cp ${root} ${prefix}.root" :"" + """ ++ $cp_cmd + cnvnator \\ + -root ${prefix}.root \\ + $args \\ +@@ -40,6 +46,7 @@ + $input_cmd \\ + $calls_cmd + ++ $mv_cmd + cat <<-END_VERSIONS > versions.yml + "${task.process}": + CNVnator: \$(echo \$(cnvnator 2>&1 | sed -n '3p' | sed 's/CNVnator v//')) + +************************************************************ diff --git a/modules/nf-core/cnvnator/cnvnator/main.nf b/modules/nf-core/cnvnator/cnvnator/main.nf index 07393639..9f08c6b2 100644 --- a/modules/nf-core/cnvnator/cnvnator/main.nf +++ b/modules/nf-core/cnvnator/cnvnator/main.nf @@ -1,6 +1,7 @@ process CNVNATOR_CNVNATOR { tag "$meta.id" label 'process_single' + stageInMode 'copy' conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? @@ -12,11 +13,12 @@ process CNVNATOR_CNVNATOR { tuple val(meta2), path(root) tuple val(meta3), path(fasta) tuple val(meta4), path(fai) + val step output: - tuple val(output_meta), path("${prefix}.root"), emit: root - tuple val(output_meta), path("${prefix}.tab") , emit: tab, optional: true - path "versions.yml" , emit: versions + tuple val(output_meta), path("${output_meta.id}_${step}.root"), emit: root + tuple val(output_meta), path("*.tab") , emit: tab, optional: true + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -32,7 +34,11 @@ process CNVNATOR_CNVNATOR { reference = '' } calls_cmd = args.contains("-call") ? "> ${prefix}.tab" : '' + mv_cmd = "mv ${prefix}.root ${prefix}_${step}.root" + steps = ["his", "stat", "partition", "call"] + cp_cmd = steps.contains(step) ? "cp ${root} ${prefix}.root" :"" """ + $cp_cmd cnvnator \\ -root ${prefix}.root \\ $args \\ @@ -40,6 +46,7 @@ process CNVNATOR_CNVNATOR { $input_cmd \\ $calls_cmd + $mv_cmd cat <<-END_VERSIONS > versions.yml "${task.process}": CNVnator: \$(echo \$(cnvnator 2>&1 | sed -n '3p' | sed 's/CNVnator v//')) diff --git a/subworkflows/local/variant_calling/call_sv_cnvnator.nf b/subworkflows/local/variant_calling/call_sv_cnvnator.nf index 0d5c5423..995b6919 100644 --- a/subworkflows/local/variant_calling/call_sv_cnvnator.nf +++ b/subworkflows/local/variant_calling/call_sv_cnvnator.nf @@ -22,11 +22,11 @@ workflow CALL_SV_CNVNATOR { main: ch_versions = Channel.empty() - CNVNATOR_RD ( ch_bam_bai, [[:],[]], [[:],[]], [[:],[]] ) - CNVNATOR_HIST ( [[:],[],[]], CNVNATOR_RD.out.root, ch_fasta, ch_fai ) - CNVNATOR_STAT ( [[:],[],[]], CNVNATOR_HIST.out.root, [[:],[]], [[:],[]] ) - CNVNATOR_PARTITION ( [[:],[],[]], CNVNATOR_STAT.out.root, [[:],[]], [[:],[]] ) - CNVNATOR_CALL ( [[:],[],[]], CNVNATOR_PARTITION.out.root, [[:],[]], [[:],[]] ) + CNVNATOR_RD ( ch_bam_bai, [[:],[]], [[:],[]], [[:],[]], "rd" ) + CNVNATOR_HIST ( [[:],[],[]], CNVNATOR_RD.out.root, ch_fasta, ch_fai, "his" ) + CNVNATOR_STAT ( [[:],[],[]], CNVNATOR_HIST.out.root, [[:],[]], [[:],[]], "stat" ) + CNVNATOR_PARTITION ( [[:],[],[]], CNVNATOR_STAT.out.root, [[:],[]], [[:],[]], "partition" ) + CNVNATOR_CALL ( [[:],[],[]], CNVNATOR_PARTITION.out.root, [[:],[]], [[:],[]], "call" ) CNVNATOR_CONVERT2VCF (CNVNATOR_CALL.out.tab).vcf .collect{it[1]} .toList() From ed71e16e69fa77d9a29ec560cc0d3532f64be2d4 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 31 Jan 2024 10:03:39 +0100 Subject: [PATCH 288/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 2a7922da..c0a0bcdf 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -47,6 +47,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - The split into clincial and research VCFs is now done before ranking the varaints [#485](https://github.com/nf-core/raredisease/pull/485) - Installed the nf-core version of ensemblvep/vep module [#482](https://github.com/nf-core/raredisease/pull/482) - The filenames of the ranked output VCF files have been changed. See [output.md](docs/output.md#filtering-and-ranking) for more information[#485](https://github.com/nf-core/raredisease/pull/485) +- Patched cnvnator module so that the processes didn't have to rerun after a failed run [#503](https://github.com/nf-core/raredisease/pull/503). ### `Fixed` From 6140c3c677fac3034f2ff7a508aab371077cea97 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 31 Jan 2024 10:11:17 +0100 Subject: [PATCH 289/387] remove stageinmode --- modules/nf-core/cnvnator/cnvnator/main.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/modules/nf-core/cnvnator/cnvnator/main.nf b/modules/nf-core/cnvnator/cnvnator/main.nf index 9f08c6b2..7c9361ef 100644 --- a/modules/nf-core/cnvnator/cnvnator/main.nf +++ b/modules/nf-core/cnvnator/cnvnator/main.nf @@ -1,7 +1,6 @@ process CNVNATOR_CNVNATOR { tag "$meta.id" label 'process_single' - stageInMode 'copy' conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? From 2869cc58913e58aa3936f95ff2e3e969b183f271 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 31 Jan 2024 10:21:00 +0100 Subject: [PATCH 290/387] update stub --- .../cnvnator/cnvnator/cnvnator-cnvnator.diff | 23 ++++++++++--------- modules/nf-core/cnvnator/cnvnator/main.nf | 2 +- 2 files changed, 13 insertions(+), 12 deletions(-) diff --git a/modules/nf-core/cnvnator/cnvnator/cnvnator-cnvnator.diff b/modules/nf-core/cnvnator/cnvnator/cnvnator-cnvnator.diff index 8186aa08..f06d7f6c 100644 --- a/modules/nf-core/cnvnator/cnvnator/cnvnator-cnvnator.diff +++ b/modules/nf-core/cnvnator/cnvnator/cnvnator-cnvnator.diff @@ -1,15 +1,7 @@ Changes in module 'nf-core/cnvnator/cnvnator' --- modules/nf-core/cnvnator/cnvnator/main.nf +++ modules/nf-core/cnvnator/cnvnator/main.nf -@@ -1,6 +1,7 @@ - process CNVNATOR_CNVNATOR { - tag "$meta.id" - label 'process_single' -+ stageInMode 'copy' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? -@@ -12,11 +13,12 @@ +@@ -12,11 +12,12 @@ tuple val(meta2), path(root) tuple val(meta3), path(fasta) tuple val(meta4), path(fai) @@ -25,7 +17,7 @@ Changes in module 'nf-core/cnvnator/cnvnator' when: task.ext.when == null || task.ext.when -@@ -32,7 +34,11 @@ +@@ -32,7 +33,11 @@ reference = '' } calls_cmd = args.contains("-call") ? "> ${prefix}.tab" : '' @@ -37,7 +29,7 @@ Changes in module 'nf-core/cnvnator/cnvnator' cnvnator \\ -root ${prefix}.root \\ $args \\ -@@ -40,6 +46,7 @@ +@@ -40,6 +45,7 @@ $input_cmd \\ $calls_cmd @@ -45,5 +37,14 @@ Changes in module 'nf-core/cnvnator/cnvnator' cat <<-END_VERSIONS > versions.yml "${task.process}": CNVnator: \$(echo \$(cnvnator 2>&1 | sed -n '3p' | sed 's/CNVnator v//')) +@@ -52,7 +58,7 @@ + output_meta = bam ? meta : meta2 + def calls_cmd = args.contains("-call") ? "touch ${prefix}.tab" : '' + """ +- touch ${prefix}.root ++ touch ${prefix}_${step}.root + $calls_cmd + + cat <<-END_VERSIONS > versions.yml ************************************************************ diff --git a/modules/nf-core/cnvnator/cnvnator/main.nf b/modules/nf-core/cnvnator/cnvnator/main.nf index 7c9361ef..66427cd1 100644 --- a/modules/nf-core/cnvnator/cnvnator/main.nf +++ b/modules/nf-core/cnvnator/cnvnator/main.nf @@ -58,7 +58,7 @@ process CNVNATOR_CNVNATOR { output_meta = bam ? meta : meta2 def calls_cmd = args.contains("-call") ? "touch ${prefix}.tab" : '' """ - touch ${prefix}.root + touch ${prefix}_${step}.root $calls_cmd cat <<-END_VERSIONS > versions.yml From 772c610c1a469eac59d6f3d19a59ad6e27b5e3bf Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 31 Jan 2024 14:25:25 +0100 Subject: [PATCH 291/387] update cadd config --- conf/modules/annotate_cadd.config | 36 +++++++++++++++---------------- 1 file changed, 17 insertions(+), 19 deletions(-) diff --git a/conf/modules/annotate_cadd.config b/conf/modules/annotate_cadd.config index 438beeb5..4ac5b618 100644 --- a/conf/modules/annotate_cadd.config +++ b/conf/modules/annotate_cadd.config @@ -16,28 +16,26 @@ // process { - if (!params.cadd_resources != "null") { - withName: '.*:ANNOTATE_CADD.*' { - ext.when = { ( !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun) } - } + withName: '.*:ANNOTATE_CADD.*' { + ext.when = { ( !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun) } + } - withName: '.*:ANNOTATE_CADD:BCFTOOLS_VIEW' { - ext.args = { "--output-type z --types indels" } - ext.prefix = { "${vcf.simpleName}_indels" } - } + withName: '.*:ANNOTATE_CADD:BCFTOOLS_VIEW' { + ext.args = { "--output-type z --types indels" } + ext.prefix = { "${vcf.simpleName}_indels" } + } - withName: '.*:ANNOTATE_CADD:CADD' { - ext.args = { "-g ${params.genome}" } - ext.prefix = { "${vcf.simpleName}_cadd" } - } + withName: '.*:ANNOTATE_CADD:CADD' { + ext.args = { "-g ${params.genome}" } + ext.prefix = { "${vcf.simpleName}_cadd" } + } - withName: '.*:ANNOTATE_CADD:TABIX_CADD' { - ext.args = { "--force --sequence 1 --begin 2 --end 2" } - } + withName: '.*:ANNOTATE_CADD:TABIX_CADD' { + ext.args = { "--force --sequence 1 --begin 2 --end 2" } + } - withName: '.*:ANNOTATE_CADD:BCFTOOLS_ANNOTATE' { - ext.args = { "--columns Chrom,Pos,Ref,Alt,-,CADD --output-type z" } - ext.prefix = { "${input.simpleName}_ann" } - } + withName: '.*:ANNOTATE_CADD:BCFTOOLS_ANNOTATE' { + ext.args = { "--columns Chrom,Pos,Ref,Alt,-,CADD --output-type z" } + ext.prefix = { "${input.simpleName}_ann" } } } From 217780b1c8e60cab7ed4457d58ed8742ec91309e Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 31 Jan 2024 15:40:04 +0100 Subject: [PATCH 292/387] fix case_channel construction --- workflows/raredisease.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 3cc83d3b..8b7e6a20 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -762,13 +762,13 @@ def create_case_channel(List rows) { if (item.phenotype == 2) { probands.add(item.sample) } - if ( (item.paternal!=0) && (item.paternal!="") && (item.maternal!=0) && (item.maternal!="") ) { + if ( (item.paternal!="0") && (item.paternal!="") && (item.maternal!="0") && (item.maternal!="") ) { upd_children.add(item.sample) } - if ( (item.paternal!=0) && (item.paternal!="") ) { + if ( (item.paternal!="0") && (item.paternal!="") ) { father = item.paternal } - if ( (item.maternal!=0) && (item.maternal!="") ) { + if ( (item.maternal!="0") && (item.maternal!="") ) { mother = item.maternal } } From 8f8f41ddfc106a3aa226584993ef1dd87bb2381c Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 31 Jan 2024 16:30:15 +0100 Subject: [PATCH 293/387] review suggestion --- conf/modules/call_repeat_expansions.config | 2 +- docs/output.md | 4 ++++ 2 files changed, 5 insertions(+), 1 deletion(-) diff --git a/conf/modules/call_repeat_expansions.config b/conf/modules/call_repeat_expansions.config index 23d3e2f8..39934a4b 100644 --- a/conf/modules/call_repeat_expansions.config +++ b/conf/modules/call_repeat_expansions.config @@ -83,7 +83,7 @@ process { } withName: '.*CALL_REPEAT_EXPANSIONS:COMPRESS_STRANGER' { - ext.prefix = { "${meta.id}_repeat_expansion" } + ext.prefix = { "${meta.id}_repeat_expansion_stranger" } ext.args = '--output-type z' publishDir = [ path: { "${params.outdir}/repeat_expansions" }, diff --git a/docs/output.md b/docs/output.md index 080c73ac..43ca906d 100644 --- a/docs/output.md +++ b/docs/output.md @@ -301,6 +301,10 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support. - `repeat_expansions/` - `_repeat_expansion.vcf.gz`: file containing variant calls. - `_repeat_expansion.vcf.gz.tbi`: index of the file containing variant calls. + - `_exphunter_sorted.bam`: A BAMlet containing alignments of reads that overlap or located in close proximity to each variant identified by ExpansionHunter + - `_exphunter_sorted.bam.bai`: Index of the BAMlet file + - `_repeat_expansion_stranger.vcf.gz`: Output from ExpansionHunter annotated with the pathologic implications of the repeat sizes by Stranger. + - `_repeat_expansion_stranger.vcf.gz`: Index of the Stranger VCF file. From a6093f41aca79c2d25777807dfb9bf9126b090f1 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 31 Jan 2024 16:47:56 +0100 Subject: [PATCH 294/387] update docs --- docs/output.md | 6 ++---- 1 file changed, 2 insertions(+), 4 deletions(-) diff --git a/docs/output.md b/docs/output.md index 43ca906d..6cc3ecf9 100644 --- a/docs/output.md +++ b/docs/output.md @@ -303,8 +303,6 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support. - `_repeat_expansion.vcf.gz.tbi`: index of the file containing variant calls. - `_exphunter_sorted.bam`: A BAMlet containing alignments of reads that overlap or located in close proximity to each variant identified by ExpansionHunter - `_exphunter_sorted.bam.bai`: Index of the BAMlet file - - `_repeat_expansion_stranger.vcf.gz`: Output from ExpansionHunter annotated with the pathologic implications of the repeat sizes by Stranger. - - `_repeat_expansion_stranger.vcf.gz`: Index of the Stranger VCF file. @@ -316,8 +314,8 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support. Output files - `repeat_expansions/` - - `_repeat_expansion.vcf.gz`: file containing variant calls. - - `_repeat_expansion.vcf.gz.tbi`: index of the file containing variant calls. + - `_repeat_expansion_stranger.vcf.gz`: file containing variant calls. + - `_repeat_expansion_stranger.vcf.gz.tbi`: index of the file containing variant calls. From a51428a081a2d41439f5ed2f2293a246e9fc0b5d Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 31 Jan 2024 21:45:56 +0100 Subject: [PATCH 295/387] chromograph viz --- conf/modules/qc_bam.config | 5 +++++ subworkflows/local/qc_bam.nf | 5 ++++- 2 files changed, 9 insertions(+), 1 deletion(-) diff --git a/conf/modules/qc_bam.config b/conf/modules/qc_bam.config index 61daf4d6..93b0f2db 100644 --- a/conf/modules/qc_bam.config +++ b/conf/modules/qc_bam.config @@ -50,6 +50,11 @@ process { ext.args = '-clip' } + withName: '.*QC_BAM:CHROMOGRAPH_COV' { + ext.args = '--euploid --step 500' + ext.prefix = { "${meta2.id}_chromographcov" } + } + withName: '.*QC_BAM:MOSDEPTH' { ext.args = '--d4' ext.prefix = { "${meta.id}_mosdepth" } diff --git a/subworkflows/local/qc_bam.nf b/subworkflows/local/qc_bam.nf index d4ae2842..9bb5a2a4 100644 --- a/subworkflows/local/qc_bam.nf +++ b/subworkflows/local/qc_bam.nf @@ -4,13 +4,14 @@ include { PICARD_COLLECTMULTIPLEMETRICS } from '../../modules/nf-core/picard/collectmultiplemetrics/main' include { PICARD_COLLECTHSMETRICS } from '../../modules/nf-core/picard/collecthsmetrics/main' +include { CHROMOGRAPH as CHROMOGRAPH_COV } from '../../modules/nf-core/chromograph/main' include { QUALIMAP_BAMQC } from '../../modules/nf-core/qualimap/bamqc/main' include { TIDDIT_COV } from '../../modules/nf-core/tiddit/cov/main' include { MOSDEPTH } from '../../modules/nf-core/mosdepth/main' include { UCSC_WIGTOBIGWIG } from '../../modules/nf-core/ucsc/wigtobigwig/main' include { PICARD_COLLECTWGSMETRICS as PICARD_COLLECTWGSMETRICS } from '../../modules/nf-core/picard/collectwgsmetrics/main' include { PICARD_COLLECTWGSMETRICS as PICARD_COLLECTWGSMETRICS_Y } from '../../modules/nf-core/picard/collectwgsmetrics/main' -include { SENTIEON_WGSMETRICS } from '../../modules/nf-core/sentieon/wgsmetrics/main' +include { SENTIEON_WGSMETRICS } from '../../modules/nf-core/sentieon/wgsmetrics/main' include { SENTIEON_WGSMETRICS as SENTIEON_WGSMETRICS_Y } from '../../modules/nf-core/sentieon/wgsmetrics/main' include { NGSBITS_SAMPLEGENDER } from '../../modules/nf-core/ngsbits/samplegender/main' @@ -51,6 +52,8 @@ workflow QC_BAM { UCSC_WIGTOBIGWIG (TIDDIT_COV.out.wig, ch_chrom_sizes) + CHROMOGRAPH_COV([[:],[]], TIDDIT_COV.out.wig, [[:],[]], [[:],[]], [[:],[]], [[:],[]], [[:],[]]) + ch_bam_bai.map{ meta, bam, bai -> [meta, bam, bai, []]}.set{ch_mosdepth_in} MOSDEPTH (ch_mosdepth_in, ch_genome_fasta) From 88b1035797d470be6e944926c25af0b661ff40b1 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 1 Feb 2024 00:23:46 +0100 Subject: [PATCH 296/387] local module --- conf/modules/call_snv_deepvariant.config | 2 +- conf/modules/call_snv_sentieon.config | 2 +- conf/modules/postprocess_MT_calls.config | 2 +- lib/WorkflowRaredisease.groovy | 15 ------ modules/local/add_varcallername_to_bed.nf | 47 +++++++++++++++++++ .../variant_calling/call_snv_deepvariant.nf | 8 ++-- .../variant_calling/call_snv_sentieon.nf | 8 ++-- .../variant_calling/postprocess_MT_calls.nf | 8 ++-- 8 files changed, 62 insertions(+), 30 deletions(-) create mode 100644 modules/local/add_varcallername_to_bed.nf diff --git a/conf/modules/call_snv_deepvariant.config b/conf/modules/call_snv_deepvariant.config index fdd7ece6..02c2b384 100644 --- a/conf/modules/call_snv_deepvariant.config +++ b/conf/modules/call_snv_deepvariant.config @@ -39,7 +39,7 @@ process { ext.prefix = { "${meta.id}_split_rmdup" } } - withName: '.*CALL_SNV_DEEPVARIANT:ZIP_TABIX_VARCALLERBED' { + withName: '.*CALL_SNV_DEEPVARIANT:ADD_VARCALLER_TO_BED' { ext.args2 = '-s 1 -b 2 -e 3' } diff --git a/conf/modules/call_snv_sentieon.config b/conf/modules/call_snv_sentieon.config index 2337ef4f..83cedb9f 100644 --- a/conf/modules/call_snv_sentieon.config +++ b/conf/modules/call_snv_sentieon.config @@ -49,7 +49,7 @@ process { ext.prefix = { "${meta.id}_split_rmdup" } } - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:ZIP_TABIX_VARCALLERBED' { + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:ADD_VARCALLER_TO_BED' { ext.args2 = '-s 1 -b 2 -e 3' } diff --git a/conf/modules/postprocess_MT_calls.config b/conf/modules/postprocess_MT_calls.config index ba6e8abf..1b4c8357 100644 --- a/conf/modules/postprocess_MT_calls.config +++ b/conf/modules/postprocess_MT_calls.config @@ -40,7 +40,7 @@ process { ext.prefix = { "${meta.id}_split_rmdup_merged" } } - withName: '.*POSTPROCESS_MT_CALLS:ZIP_TABIX_VARCALLERBED' { + withName: '.*POSTPROCESS_MT_CALLS:ADD_VARCALLER_TO_BED' { ext.args2 = '-s 1 -b 2 -e 3' } diff --git a/lib/WorkflowRaredisease.groovy b/lib/WorkflowRaredisease.groovy index 578dc6d6..f45fcc7c 100755 --- a/lib/WorkflowRaredisease.groovy +++ b/lib/WorkflowRaredisease.groovy @@ -35,21 +35,6 @@ class WorkflowRaredisease { return outfile } - // - // Create a bed file which includes the name of variant caller from chromsizes file - // - public static String makeBedWithVariantCallerInfo(chromsizes, varcaller) { - def parent_dir = chromsizes.parent.toString() - def outfile = new File(parent_dir + '/' + varcaller +'.bed') - def writer = outfile.newWriter() - chromsizes.eachLine { line -> - def split_str = line.tokenize("\t") - writer << [split_str[0],"0",split_str[1],varcaller].join("\t") + "\n" - } - writer.close() - return outfile - } - // // Get workflow summary for MultiQC // diff --git a/modules/local/add_varcallername_to_bed.nf b/modules/local/add_varcallername_to_bed.nf new file mode 100644 index 00000000..b9d57d5d --- /dev/null +++ b/modules/local/add_varcallername_to_bed.nf @@ -0,0 +1,47 @@ +process ADD_VARCALLER_TO_BED { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : + 'biocontainers/tabix:1.11--hdfd78af_0' }" + + input: + tuple val(meta), path(chromsizes) + + output: + tuple val(meta), path("*.gz"), path("*.tbi"), optional: true, emit: gz_tbi + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def variant_caller = "${meta.id}" + """ + awk '{print \$1"\t0\t"\$2"\t$variant_caller\"}' $chromsizes > ${variant_caller}.bed + bgzip --threads ${task.cpus} -c $args ${variant_caller}.bed > ${prefix}.bed.gz + tabix $args2 ${prefix}.bed.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bed.gz + touch ${prefix}.bed.gz.tbi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/subworkflows/local/variant_calling/call_snv_deepvariant.nf b/subworkflows/local/variant_calling/call_snv_deepvariant.nf index b20e19ae..4fc6ab4d 100644 --- a/subworkflows/local/variant_calling/call_snv_deepvariant.nf +++ b/subworkflows/local/variant_calling/call_snv_deepvariant.nf @@ -7,9 +7,9 @@ include { BCFTOOLS_NORM as SPLIT_MULTIALLELICS_GL } from '../../../modules/nf include { BCFTOOLS_NORM as REMOVE_DUPLICATES_GL } from '../../../modules/nf-core/bcftools/norm/main' include { DEEPVARIANT } from '../../../modules/nf-core/deepvariant/main' include { GLNEXUS } from '../../../modules/nf-core/glnexus/main' -include { TABIX_BGZIPTABIX as ZIP_TABIX_VARCALLERBED } from '../../../modules/nf-core/tabix/bgziptabix/main' include { TABIX_TABIX as TABIX_GL } from '../../../modules/nf-core/tabix/tabix/main' include { TABIX_TABIX as TABIX_ANNOTATE } from '../../../modules/nf-core/tabix/tabix/main' +include { ADD_VARCALLER_TO_BED } from '../../../modules/local/add_varcallername_to_bed' workflow CALL_SNV_DEEPVARIANT { take: @@ -54,11 +54,11 @@ workflow CALL_SNV_DEEPVARIANT { TABIX_GL (REMOVE_DUPLICATES_GL.out.vcf) ch_genome_chrsizes.flatten().map{chromsizes -> - return [[id:'deepvariant'], WorkflowRaredisease.makeBedWithVariantCallerInfo(chromsizes, "deepvariant")] + return [[id:'deepvariant'], chromsizes] } .set { ch_varcallerinfo } - ZIP_TABIX_VARCALLERBED (ch_varcallerinfo).gz_tbi + ADD_VARCALLER_TO_BED (ch_varcallerinfo).gz_tbi .map{meta,bed,tbi -> return [bed, tbi]} .set{ch_varcallerbed} @@ -77,7 +77,7 @@ workflow CALL_SNV_DEEPVARIANT { ch_versions = ch_versions.mix(SPLIT_MULTIALLELICS_GL.out.versions) ch_versions = ch_versions.mix(REMOVE_DUPLICATES_GL.out.versions) ch_versions = ch_versions.mix(TABIX_GL.out.versions) - ch_versions = ch_versions.mix(ZIP_TABIX_VARCALLERBED.out.versions) + ch_versions = ch_versions.mix(ADD_VARCALLER_TO_BED.out.versions) ch_versions = ch_versions.mix(BCFTOOLS_ANNOTATE.out.versions) ch_versions = ch_versions.mix(TABIX_ANNOTATE.out.versions) diff --git a/subworkflows/local/variant_calling/call_snv_sentieon.nf b/subworkflows/local/variant_calling/call_snv_sentieon.nf index 53db6095..d0586c82 100644 --- a/subworkflows/local/variant_calling/call_snv_sentieon.nf +++ b/subworkflows/local/variant_calling/call_snv_sentieon.nf @@ -13,7 +13,7 @@ include { BCFTOOLS_FILTER as BCF_FILTER_ONE } from '../../../modules/nf include { BCFTOOLS_FILTER as BCF_FILTER_TWO } from '../../../modules/nf-core/bcftools/filter/main' include { BCFTOOLS_ANNOTATE } from '../../../modules/nf-core/bcftools/annotate/main' include { TABIX_TABIX as TABIX_ANNOTATE } from '../../../modules/nf-core/tabix/tabix/main' -include { TABIX_BGZIPTABIX as ZIP_TABIX_VARCALLERBED } from '../../../modules/nf-core/tabix/bgziptabix/main' +include { ADD_VARCALLER_TO_BED } from '../../../modules/local/add_varcallername_to_bed' workflow CALL_SNV_SENTIEON { take: @@ -91,11 +91,11 @@ workflow CALL_SNV_SENTIEON { TABIX_SEN(REMOVE_DUPLICATES_SEN.out.vcf) ch_genome_chrsizes.flatten().map{chromsizes -> - return [[id:'sentieon_dnascope'], WorkflowRaredisease.makeBedWithVariantCallerInfo(chromsizes, "sentieon_dnascope")] + return [[id:'sentieon_dnascope'], chromsizes] } .set { ch_varcallerinfo } - ZIP_TABIX_VARCALLERBED (ch_varcallerinfo).gz_tbi + ADD_VARCALLER_TO_BED (ch_varcallerinfo).gz_tbi .map{meta,bed,tbi -> return [bed, tbi]} .set{ch_varcallerbed} @@ -116,7 +116,7 @@ workflow CALL_SNV_SENTIEON { ch_versions = ch_versions.mix(REMOVE_DUPLICATES_SEN.out.versions.first()) ch_versions = ch_versions.mix(TABIX_SEN.out.versions.first()) ch_versions = ch_versions.mix(BCF_FILTER_ONE.out.versions.first()) - ch_versions = ch_versions.mix(ZIP_TABIX_VARCALLERBED.out.versions) + ch_versions = ch_versions.mix(ADD_VARCALLER_TO_BED.out.versions) ch_versions = ch_versions.mix(BCFTOOLS_ANNOTATE.out.versions) ch_versions = ch_versions.mix(TABIX_ANNOTATE.out.versions) diff --git a/subworkflows/local/variant_calling/postprocess_MT_calls.nf b/subworkflows/local/variant_calling/postprocess_MT_calls.nf index 81279a9c..4cd79882 100644 --- a/subworkflows/local/variant_calling/postprocess_MT_calls.nf +++ b/subworkflows/local/variant_calling/postprocess_MT_calls.nf @@ -12,7 +12,7 @@ include { BCFTOOLS_MERGE as BCFTOOLS_MERGE_MT } from '../../.. include { TABIX_TABIX as TABIX_TABIX_MERGE } from '../../../modules/nf-core/tabix/tabix/main' include { PICARD_LIFTOVERVCF } from '../../../modules/nf-core/picard/liftovervcf/main' include { BCFTOOLS_ANNOTATE } from '../../../modules/nf-core/bcftools/annotate/main' -include { TABIX_BGZIPTABIX as ZIP_TABIX_VARCALLERBED } from '../../../modules/nf-core/tabix/bgziptabix/main' +include { ADD_VARCALLER_TO_BED } from '../../../modules/local/add_varcallername_to_bed' include { TABIX_TABIX as TABIX_ANNOTATE } from '../../../modules/nf-core/tabix/tabix/main' workflow POSTPROCESS_MT_CALLS { @@ -103,11 +103,11 @@ workflow POSTPROCESS_MT_CALLS { TABIX_TABIX_MERGE(ch_addfoundintag_in) ch_genome_chrsizes.flatten().map{chromsizes -> - return [[id:'mutect2'], WorkflowRaredisease.makeBedWithVariantCallerInfo(chromsizes, "mutect2")] + return [[id:'mutect2'], chromsizes] } .set { ch_varcallerinfo } - ZIP_TABIX_VARCALLERBED (ch_varcallerinfo).gz_tbi + ADD_VARCALLER_TO_BED (ch_varcallerinfo).gz_tbi .map{meta,bed,tbi -> return [bed, tbi]} .set{ch_varcallerbed} @@ -127,7 +127,7 @@ workflow POSTPROCESS_MT_CALLS { ch_versions = ch_versions.mix(SPLIT_MULTIALLELICS_MT.out.versions.first()) ch_versions = ch_versions.mix(REMOVE_DUPLICATES_MT.out.versions.first()) ch_versions = ch_versions.mix(BCFTOOLS_MERGE_MT.out.versions) - ch_versions = ch_versions.mix(ZIP_TABIX_VARCALLERBED.out.versions) + ch_versions = ch_versions.mix(ADD_VARCALLER_TO_BED.out.versions) ch_versions = ch_versions.mix(BCFTOOLS_ANNOTATE.out.versions) ch_versions = ch_versions.mix(TABIX_ANNOTATE.out.versions) From 1a3db637b6a99202ed2d6e36cdd4bd9f15ab55d6 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 1 Feb 2024 00:27:59 +0100 Subject: [PATCH 297/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index c0a0bcdf..56899343 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -48,6 +48,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Installed the nf-core version of ensemblvep/vep module [#482](https://github.com/nf-core/raredisease/pull/482) - The filenames of the ranked output VCF files have been changed. See [output.md](docs/output.md#filtering-and-ranking) for more information[#485](https://github.com/nf-core/raredisease/pull/485) - Patched cnvnator module so that the processes didn't have to rerun after a failed run [#503](https://github.com/nf-core/raredisease/pull/503). +- Added a local module to generate bed files with variant caller names [#503](https://github.com/nf-core/raredisease/pull/505). ### `Fixed` From 06c211fe3383c6d729f647d07acd772f349db04d Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 1 Feb 2024 13:16:21 +0100 Subject: [PATCH 298/387] Update CHANGELOG.md Co-authored-by: Anders Jemt --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 56899343..55ea2691 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -48,7 +48,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Installed the nf-core version of ensemblvep/vep module [#482](https://github.com/nf-core/raredisease/pull/482) - The filenames of the ranked output VCF files have been changed. See [output.md](docs/output.md#filtering-and-ranking) for more information[#485](https://github.com/nf-core/raredisease/pull/485) - Patched cnvnator module so that the processes didn't have to rerun after a failed run [#503](https://github.com/nf-core/raredisease/pull/503). -- Added a local module to generate bed files with variant caller names [#503](https://github.com/nf-core/raredisease/pull/505). +- Added a local module to generate bed files with variant caller names [#505](https://github.com/nf-core/raredisease/pull/505). ### `Fixed` From 9f72dc4d36518c7c4035780e6ab58e358d58a5a9 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 1 Feb 2024 13:16:35 +0100 Subject: [PATCH 299/387] Update modules/local/add_varcallername_to_bed.nf Co-authored-by: Anders Jemt --- modules/local/add_varcallername_to_bed.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/add_varcallername_to_bed.nf b/modules/local/add_varcallername_to_bed.nf index b9d57d5d..11190c2a 100644 --- a/modules/local/add_varcallername_to_bed.nf +++ b/modules/local/add_varcallername_to_bed.nf @@ -11,7 +11,7 @@ process ADD_VARCALLER_TO_BED { tuple val(meta), path(chromsizes) output: - tuple val(meta), path("*.gz"), path("*.tbi"), optional: true, emit: gz_tbi + tuple val(meta), path("*.gz"), path("*.tbi"), emit: gz_tbi path "versions.yml" , emit: versions when: From bed0a9af2bc7ecfecd67501acec4402a0cc5a46f Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 1 Feb 2024 13:35:59 +0100 Subject: [PATCH 300/387] update modules --- modules.json | 16 ++++++++-------- 1 file changed, 8 insertions(+), 8 deletions(-) diff --git a/modules.json b/modules.json index ff937852..ad60caa0 100644 --- a/modules.json +++ b/modules.json @@ -133,7 +133,7 @@ }, "fastqc": { "branch": "master", - "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["modules"] }, "gatk4/bedtointervallist": { @@ -240,22 +240,22 @@ }, "genmod/annotate": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "1aba459a6f3528bee806403ae47bea304de26603", "installed_by": ["modules"] }, "genmod/compound": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "1aba459a6f3528bee806403ae47bea304de26603", "installed_by": ["modules"] }, "genmod/models": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "1aba459a6f3528bee806403ae47bea304de26603", "installed_by": ["modules"] }, "genmod/score": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "1aba459a6f3528bee806403ae47bea304de26603", "installed_by": ["modules"] }, "glnexus": { @@ -330,7 +330,7 @@ }, "picard/markduplicates": { "branch": "master", - "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447", + "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", "installed_by": ["modules"] }, "picard/renamesampleinvcf": { @@ -345,7 +345,7 @@ }, "qualimap/bamqc": { "branch": "master", - "git_sha": "260677adfe5eecf5f64d3db726f6854d4497b49b", + "git_sha": "6b0e4fe14ca1b12e131f64608f0bbaf36fd11451", "installed_by": ["modules"] }, "rhocall/annotate": { @@ -385,7 +385,7 @@ }, "samtools/stats": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", "installed_by": ["modules"] }, "samtools/view": { From faf45a3c56fc80513469b8478a707605091a5ea3 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 1 Feb 2024 13:36:14 +0100 Subject: [PATCH 301/387] update modules --- modules/nf-core/fastqc/tests/main.nf.test | 14 +-- .../nf-core/fastqc/tests/main.nf.test.snap | 76 ++++++++++++++++- .../nf-core/genmod/annotate/environment.yml | 2 +- modules/nf-core/genmod/annotate/main.nf | 4 +- modules/nf-core/genmod/annotate/meta.yml | 5 +- .../genmod/annotate/tests/main.nf.test | 34 ++++++++ .../genmod/annotate/tests/nextflow.config | 5 ++ .../nf-core/genmod/annotate/tests/tags.yml | 2 + .../nf-core/genmod/compound/environment.yml | 2 +- modules/nf-core/genmod/compound/main.nf | 4 +- modules/nf-core/genmod/compound/meta.yml | 3 +- .../genmod/compound/tests/main.nf.test | 33 +++++++ .../nf-core/genmod/compound/tests/tags.yml | 2 + modules/nf-core/genmod/models/environment.yml | 3 +- modules/nf-core/genmod/models/main.nf | 4 +- modules/nf-core/genmod/models/meta.yml | 3 +- .../nf-core/genmod/models/tests/main.nf.test | 35 ++++++++ modules/nf-core/genmod/models/tests/tags.yml | 2 + modules/nf-core/genmod/score/environment.yml | 2 +- modules/nf-core/genmod/score/main.nf | 4 +- modules/nf-core/genmod/score/meta.yml | 3 +- .../nf-core/genmod/score/tests/main.nf.test | 35 ++++++++ modules/nf-core/genmod/score/tests/tags.yml | 2 + .../picard/markduplicates/tests/main.nf.test | 85 +++++++++---------- .../markduplicates/tests/main.nf.test.snap | 66 ++++++++++---- .../nf-core/qualimap/bamqc/tests/main.nf.test | 18 ++-- .../qualimap/bamqc/tests/main.nf.test.snap | 13 ++- .../nf-core/samtools/stats/environment.yml | 1 + 28 files changed, 356 insertions(+), 106 deletions(-) create mode 100644 modules/nf-core/genmod/annotate/tests/main.nf.test create mode 100644 modules/nf-core/genmod/annotate/tests/nextflow.config create mode 100644 modules/nf-core/genmod/annotate/tests/tags.yml create mode 100644 modules/nf-core/genmod/compound/tests/main.nf.test create mode 100644 modules/nf-core/genmod/compound/tests/tags.yml create mode 100644 modules/nf-core/genmod/models/tests/main.nf.test create mode 100644 modules/nf-core/genmod/models/tests/tags.yml create mode 100644 modules/nf-core/genmod/score/tests/main.nf.test create mode 100644 modules/nf-core/genmod/score/tests/tags.yml diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 1f21c664..70edae4d 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -33,7 +33,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_single") } ) } } @@ -63,7 +63,7 @@ nextflow_process { { assert path(process.out.html[0][1][0]).text.contains("") }, { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } ) } } @@ -89,7 +89,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } ) } } @@ -115,7 +115,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } ) } } @@ -153,7 +153,7 @@ nextflow_process { { assert path(process.out.html[0][1][2]).text.contains("") }, { assert path(process.out.html[0][1][3]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } ) } } @@ -179,7 +179,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } ) } } @@ -204,7 +204,7 @@ nextflow_process { { assert process.success }, { assert snapshot(process.out.html.collect { file(it[1]).getName() } + process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match() } + process.out.versions ).match("fastqc_stub") } ) } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 5d624bb8..86f7c311 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,5 +1,17 @@ { - "sarscov2 single-end [fastq] - stub": { + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { "content": [ [ "test.html", @@ -7,14 +19,70 @@ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:40:57.254299" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" }, - "versions": { + "fastqc_versions_custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:36:50.033627" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" } } \ No newline at end of file diff --git a/modules/nf-core/genmod/annotate/environment.yml b/modules/nf-core/genmod/annotate/environment.yml index 6ca55014..ac8140fd 100644 --- a/modules/nf-core/genmod/annotate/environment.yml +++ b/modules/nf-core/genmod/annotate/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::genmod=3.7.4 + - bioconda::genmod=3.8.2 diff --git a/modules/nf-core/genmod/annotate/main.nf b/modules/nf-core/genmod/annotate/main.nf index 13b7aff8..eb161187 100644 --- a/modules/nf-core/genmod/annotate/main.nf +++ b/modules/nf-core/genmod/annotate/main.nf @@ -4,8 +4,8 @@ process GENMOD_ANNOTATE { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/genmod:3.7.4--pyh5e36f6f_0': - 'biocontainers/genmod:3.7.4--pyh5e36f6f_0' }" + 'https://depot.galaxyproject.org/singularity/genmod:3.8.2--pyhdfd78af_0': + 'biocontainers/genmod:3.8.2--pyhdfd78af_0' }" input: tuple val(meta), path(input_vcf) diff --git a/modules/nf-core/genmod/annotate/meta.yml b/modules/nf-core/genmod/annotate/meta.yml index 81aac686..3c876ac5 100644 --- a/modules/nf-core/genmod/annotate/meta.yml +++ b/modules/nf-core/genmod/annotate/meta.yml @@ -3,13 +3,14 @@ description: for annotating regions, frequencies, cadd scores keywords: - annotate - genmod + - ranking tools: - "genmod": description: "Annotate genetic inheritance models in variant files" homepage: "https://github.com/Clinical-Genomics/genmod" documentation: "https://github.com/Clinical-Genomics/genmod" tool_dev_url: "https://github.com/moonso" - licence: "['MIT']" + licence: ["MIT"] input: - meta: type: map @@ -31,7 +32,7 @@ output: description: File containing software versions pattern: "versions.yml" - vcf: - type: vcf + type: file description: Annotated VCF file pattern: "*.{vcf}" authors: diff --git a/modules/nf-core/genmod/annotate/tests/main.nf.test b/modules/nf-core/genmod/annotate/tests/main.nf.test new file mode 100644 index 00000000..746de32b --- /dev/null +++ b/modules/nf-core/genmod/annotate/tests/main.nf.test @@ -0,0 +1,34 @@ +nextflow_process { + + name "Test Process GENMOD_ANNOTATE" + script "modules/nf-core/genmod/annotate/main.nf" + process "GENMOD_ANNOTATE" + config "./nextflow.config" + tag "modules" + tag "modules_nfcore" + tag "genmod" + tag "genmod/annotate" + + test("genmod_annotate") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.test_data['homo_sapiens']['illumina']['genmod_vcf_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert path(process.out.vcf.get(0).get(1)).readLines().contains("##fileformat=VCFv4.2")} + ) + } + + } + +} diff --git a/modules/nf-core/genmod/annotate/tests/nextflow.config b/modules/nf-core/genmod/annotate/tests/nextflow.config new file mode 100644 index 00000000..a1860460 --- /dev/null +++ b/modules/nf-core/genmod/annotate/tests/nextflow.config @@ -0,0 +1,5 @@ +process{ + withName: GENMOD_ANNOTATE { + ext.args = " --annotate_regions " + } +} diff --git a/modules/nf-core/genmod/annotate/tests/tags.yml b/modules/nf-core/genmod/annotate/tests/tags.yml new file mode 100644 index 00000000..88bb35a1 --- /dev/null +++ b/modules/nf-core/genmod/annotate/tests/tags.yml @@ -0,0 +1,2 @@ +genmod/annotate: + - modules/nf-core/genmod/annotate/** diff --git a/modules/nf-core/genmod/compound/environment.yml b/modules/nf-core/genmod/compound/environment.yml index 565143f9..1e2561fd 100644 --- a/modules/nf-core/genmod/compound/environment.yml +++ b/modules/nf-core/genmod/compound/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::genmod=3.7.4 + - bioconda::genmod=3.8.2 diff --git a/modules/nf-core/genmod/compound/main.nf b/modules/nf-core/genmod/compound/main.nf index d58f5fc4..1731b722 100644 --- a/modules/nf-core/genmod/compound/main.nf +++ b/modules/nf-core/genmod/compound/main.nf @@ -4,8 +4,8 @@ process GENMOD_COMPOUND { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/genmod:3.7.4--pyh5e36f6f_0': - 'biocontainers/genmod:3.7.4--pyh5e36f6f_0' }" + 'https://depot.galaxyproject.org/singularity/genmod:3.8.2--pyhdfd78af_0': + 'biocontainers/genmod:3.8.2--pyhdfd78af_0' }" input: tuple val(meta), path(input_vcf) diff --git a/modules/nf-core/genmod/compound/meta.yml b/modules/nf-core/genmod/compound/meta.yml index 378bedfc..aa5f7da5 100644 --- a/modules/nf-core/genmod/compound/meta.yml +++ b/modules/nf-core/genmod/compound/meta.yml @@ -3,13 +3,14 @@ description: Score compounds keywords: - compound - genmod + - ranking tools: - "genmod": description: "Annotate genetic inheritance models in variant files" homepage: "https://github.com/Clinical-Genomics/genmod" documentation: "https://github.com/Clinical-Genomics/genmod" tool_dev_url: "https://github.com/moonso" - licence: "['MIT']" + licence: ["MIT"] input: - meta: type: map diff --git a/modules/nf-core/genmod/compound/tests/main.nf.test b/modules/nf-core/genmod/compound/tests/main.nf.test new file mode 100644 index 00000000..cf234186 --- /dev/null +++ b/modules/nf-core/genmod/compound/tests/main.nf.test @@ -0,0 +1,33 @@ +nextflow_process { + + name "Test Process GENMOD_COMPOUND" + script "modules/nf-core/genmod/compound/main.nf" + process "GENMOD_COMPOUND" + tag "modules" + tag "modules_nfcore" + tag "genmod" + tag "genmod/compound" + + test("genmod_compound") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.test_data['homo_sapiens']['genome']['genmod_compound_vcf_mt'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert path(process.out.vcf.get(0).get(1)).readLines().contains("##fileformat=VCFv4.2")} + ) + } + + } + +} diff --git a/modules/nf-core/genmod/compound/tests/tags.yml b/modules/nf-core/genmod/compound/tests/tags.yml new file mode 100644 index 00000000..870b35e8 --- /dev/null +++ b/modules/nf-core/genmod/compound/tests/tags.yml @@ -0,0 +1,2 @@ +genmod/compound: + - modules/nf-core/genmod/compound/** diff --git a/modules/nf-core/genmod/models/environment.yml b/modules/nf-core/genmod/models/environment.yml index ff0de822..62a746c7 100644 --- a/modules/nf-core/genmod/models/environment.yml +++ b/modules/nf-core/genmod/models/environment.yml @@ -4,5 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::genmod=3.7.4 - - conda-forge::python=3.4.5 + - bioconda::genmod=3.8.2 diff --git a/modules/nf-core/genmod/models/main.nf b/modules/nf-core/genmod/models/main.nf index 15dc077b..0504574a 100644 --- a/modules/nf-core/genmod/models/main.nf +++ b/modules/nf-core/genmod/models/main.nf @@ -4,8 +4,8 @@ process GENMOD_MODELS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/genmod:3.7.4--pyh5e36f6f_0': - 'biocontainers/genmod:3.7.4--pyh5e36f6f_0' }" + 'https://depot.galaxyproject.org/singularity/genmod:3.8.2--pyhdfd78af_0': + 'biocontainers/genmod:3.8.2--pyhdfd78af_0' }" input: tuple val(meta), path(input_vcf) diff --git a/modules/nf-core/genmod/models/meta.yml b/modules/nf-core/genmod/models/meta.yml index ea3295ac..dd9001e0 100644 --- a/modules/nf-core/genmod/models/meta.yml +++ b/modules/nf-core/genmod/models/meta.yml @@ -3,13 +3,14 @@ description: annotate models of inheritance keywords: - models - genmod + - ranking tools: - "genmod": description: "Annotate genetic inheritance models in variant files" homepage: "https://github.com/Clinical-Genomics/genmod" documentation: "https://github.com/Clinical-Genomics/genmod" tool_dev_url: "https://github.com/moonso" - licence: "['MIT']" + licence: ["MIT"] input: - meta: type: map diff --git a/modules/nf-core/genmod/models/tests/main.nf.test b/modules/nf-core/genmod/models/tests/main.nf.test new file mode 100644 index 00000000..a69bb136 --- /dev/null +++ b/modules/nf-core/genmod/models/tests/main.nf.test @@ -0,0 +1,35 @@ +nextflow_process { + + name "Test Process GENMOD_MODELS" + script "modules/nf-core/genmod/models/main.nf" + process "GENMOD_MODELS" + tag "modules" + tag "modules_nfcore" + tag "genmod" + tag "genmod/models" + + test("genmod_models") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['genmod_annotate_vcf_gz'], checkIfExists: true) + ] + input[1] = file(params.test_data['homo_sapiens']['genome']['justhusky_ped'], checkIfExists: true) + input[2] = [] + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert path(process.out.vcf.get(0).get(1)).readLines().contains("##fileformat=VCFv4.2")} + ) + } + + } + +} diff --git a/modules/nf-core/genmod/models/tests/tags.yml b/modules/nf-core/genmod/models/tests/tags.yml new file mode 100644 index 00000000..72b3b6bb --- /dev/null +++ b/modules/nf-core/genmod/models/tests/tags.yml @@ -0,0 +1,2 @@ +genmod/models: + - modules/nf-core/genmod/models/** diff --git a/modules/nf-core/genmod/score/environment.yml b/modules/nf-core/genmod/score/environment.yml index a09ee359..bacc4cec 100644 --- a/modules/nf-core/genmod/score/environment.yml +++ b/modules/nf-core/genmod/score/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::genmod=3.7.4 + - bioconda::genmod=3.8.2 diff --git a/modules/nf-core/genmod/score/main.nf b/modules/nf-core/genmod/score/main.nf index 362ca074..15be5f74 100644 --- a/modules/nf-core/genmod/score/main.nf +++ b/modules/nf-core/genmod/score/main.nf @@ -4,8 +4,8 @@ process GENMOD_SCORE { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/genmod:3.7.4--pyh5e36f6f_0': - 'biocontainers/genmod:3.7.4--pyh5e36f6f_0' }" + 'https://depot.galaxyproject.org/singularity/genmod:3.8.2--pyhdfd78af_0': + 'biocontainers/genmod:3.8.2--pyhdfd78af_0' }" input: tuple val(meta), path(input_vcf) diff --git a/modules/nf-core/genmod/score/meta.yml b/modules/nf-core/genmod/score/meta.yml index 49dddcb1..8998b00c 100644 --- a/modules/nf-core/genmod/score/meta.yml +++ b/modules/nf-core/genmod/score/meta.yml @@ -2,6 +2,7 @@ name: "genmod_score" description: Score the variants of a vcf based on their annotation keywords: - score + - ranking - genmod tools: - "genmod": @@ -9,7 +10,7 @@ tools: homepage: "https://github.com/Clinical-Genomics/genmod" documentation: "https://github.com/Clinical-Genomics/genmod" tool_dev_url: "https://github.com/moonso" - licence: "['MIT']" + licence: ["MIT"] input: - meta: type: map diff --git a/modules/nf-core/genmod/score/tests/main.nf.test b/modules/nf-core/genmod/score/tests/main.nf.test new file mode 100644 index 00000000..d9296fb4 --- /dev/null +++ b/modules/nf-core/genmod/score/tests/main.nf.test @@ -0,0 +1,35 @@ +nextflow_process { + + name "Test Process GENMOD_SCORE" + script "modules/nf-core/genmod/score/main.nf" + process "GENMOD_SCORE" + tag "modules" + tag "modules_nfcore" + tag "genmod" + tag "genmod/score" + + test("genmod_score") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['genmod_models_vcf_gz'], checkIfExists: true) + ] + input[1] = file(params.test_data['homo_sapiens']['genome']['justhusky_ped'], checkIfExists: true) + input[2] = file(params.test_data['homo_sapiens']['illumina']['rank_model'], checkIfExists: true) + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert path(process.out.vcf.get(0).get(1)).readLines().contains("##fileformat=VCFv4.2")} + ) + } + + } + +} diff --git a/modules/nf-core/genmod/score/tests/tags.yml b/modules/nf-core/genmod/score/tests/tags.yml new file mode 100644 index 00000000..cef831ed --- /dev/null +++ b/modules/nf-core/genmod/score/tests/tags.yml @@ -0,0 +1,2 @@ +genmod/score: + - modules/nf-core/genmod/score/** diff --git a/modules/nf-core/picard/markduplicates/tests/main.nf.test b/modules/nf-core/picard/markduplicates/tests/main.nf.test index b2bba094..c5a29b4b 100644 --- a/modules/nf-core/picard/markduplicates/tests/main.nf.test +++ b/modules/nf-core/picard/markduplicates/tests/main.nf.test @@ -9,23 +9,23 @@ nextflow_process { tag "picard" tag "picard/markduplicates" - test("sarscov2 - bam, fasta, fai - sorted bam") { + test("sarscov2 [unsorted bam]") { when { process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) - ] - input[1] = [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true) + ]) + input[1] = Channel.of([ [ id:'genome' ], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - input[2] = [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of([ [ id:'genome' ], - file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) - ] + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) + ]) """ } } @@ -33,32 +33,30 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - path(process.out.metrics.get(0).get(1)).readLines()[0..2], - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("unsorted_bam_name") }, + { assert snapshot(path(process.out.metrics.get(0).get(1)).readLines()[0..2]).match("unsorted_bam_metrics") }, + { assert snapshot(process.out.versions).match("unsorted_bam_versions") } ) } } - test("sarscov2 - bam, fasta, fai - unsorted bam") { + test("sarscov2 [sorted bam]") { when { process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true) - ] - input[1] = [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + input[1] = Channel.of([ [ id:'genome' ], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - input[2] = [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of([ [ id:'genome' ], - file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) - ] + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) + ]) """ } } @@ -66,32 +64,30 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - path(process.out.metrics.get(0).get(1)).readLines()[0..2], - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("sorted_bam_name") }, + { assert snapshot(path(process.out.metrics.get(0).get(1)).readLines()[0..2]).match("sorted_bam_metrics") }, + { assert snapshot(process.out.versions).match("sorted_bam_versions") } ) } } - test("homo_sapiens - cram, fasta, fai") { + test("homo_sapiens [cram]") { when { process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true) - ] - input[1] = [ + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true) + ]) + input[1] = Channel.of([ [ id:'genome' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - input[2] = [ + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of([ [ id:'genome' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) - ] + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) + ]) """ } } @@ -99,13 +95,10 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - path(process.out.metrics.get(0).get(1)).readLines()[0..2], - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("cram_name") }, + { assert snapshot(path(process.out.metrics.get(0).get(1)).readLines()[0..2]).match("cram_metrics") }, + { assert snapshot(process.out.versions).match("cram_versions") } ) } } - } diff --git a/modules/nf-core/picard/markduplicates/tests/main.nf.test.snap b/modules/nf-core/picard/markduplicates/tests/main.nf.test.snap index cd788a4d..31c9130d 100644 --- a/modules/nf-core/picard/markduplicates/tests/main.nf.test.snap +++ b/modules/nf-core/picard/markduplicates/tests/main.nf.test.snap @@ -1,44 +1,74 @@ { - "sarscov2 - bam, fasta, fai - unsorted bam": { + "sorted_bam_versions": { "content": [ - "test.marked.bam", - [ - "## htsjdk.samtools.metrics.StringHeader", - "# MarkDuplicates --INPUT test.paired_end.bam --OUTPUT test.marked.bam --METRICS_FILE test.marked.MarkDuplicates.metrics.txt --ASSUME_SORT_ORDER queryname --REFERENCE_SEQUENCE genome.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --REMOVE_DUPLICATES false --ASSUME_SORTED false --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --VALIDATION_STRINGENCY STRICT --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false", - "## htsjdk.samtools.metrics.StringHeader" - ], [ "versions.yml:md5,b699af51b1956f3810f8a7c066e0ab17" ] ], - "timestamp": "2023-11-28T10:50:37.735339781" + "timestamp": "2024-01-19T10:26:45.092349" }, - "homo_sapiens - cram, fasta, fai": { + "unsorted_bam_name": { + "content": [ + "test.marked.bam" + ], + "timestamp": "2024-01-19T10:26:28.100755" + }, + "cram_metrics": { "content": [ - "test.marked.bam", [ "## htsjdk.samtools.metrics.StringHeader", "# MarkDuplicates --INPUT test.paired_end.sorted.cram --OUTPUT test.marked.bam --METRICS_FILE test.marked.MarkDuplicates.metrics.txt --ASSUME_SORT_ORDER queryname --REFERENCE_SEQUENCE genome.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --REMOVE_DUPLICATES false --ASSUME_SORTED false --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --VALIDATION_STRINGENCY STRICT --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false", "## htsjdk.samtools.metrics.StringHeader" - ], - [ - "versions.yml:md5,b699af51b1956f3810f8a7c066e0ab17" ] ], - "timestamp": "2023-11-28T10:50:48.897954543" + "timestamp": "2024-01-19T10:27:03.253071" }, - "sarscov2 - bam, fasta, fai - sorted bam": { + "sorted_bam_metrics": { "content": [ - "test.marked.bam", [ "## htsjdk.samtools.metrics.StringHeader", "# MarkDuplicates --INPUT test.paired_end.sorted.bam --OUTPUT test.marked.bam --METRICS_FILE test.marked.MarkDuplicates.metrics.txt --ASSUME_SORT_ORDER queryname --REFERENCE_SEQUENCE genome.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --REMOVE_DUPLICATES false --ASSUME_SORTED false --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --VALIDATION_STRINGENCY STRICT --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false", "## htsjdk.samtools.metrics.StringHeader" - ], + ] + ], + "timestamp": "2024-01-19T10:26:45.086503" + }, + "cram_name": { + "content": [ + "test.marked.bam" + ], + "timestamp": "2024-01-19T10:27:03.241617" + }, + "cram_versions": { + "content": [ [ "versions.yml:md5,b699af51b1956f3810f8a7c066e0ab17" ] ], - "timestamp": "2023-11-28T10:50:26.591387512" + "timestamp": "2024-01-19T10:27:03.26989" + }, + "unsorted_bam_versions": { + "content": [ + [ + "versions.yml:md5,b699af51b1956f3810f8a7c066e0ab17" + ] + ], + "timestamp": "2024-01-19T10:26:28.159071" + }, + "unsorted_bam_metrics": { + "content": [ + [ + "## htsjdk.samtools.metrics.StringHeader", + "# MarkDuplicates --INPUT test.paired_end.bam --OUTPUT test.marked.bam --METRICS_FILE test.marked.MarkDuplicates.metrics.txt --ASSUME_SORT_ORDER queryname --REFERENCE_SEQUENCE genome.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --REMOVE_DUPLICATES false --ASSUME_SORTED false --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --VALIDATION_STRINGENCY STRICT --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false", + "## htsjdk.samtools.metrics.StringHeader" + ] + ], + "timestamp": "2024-01-19T10:26:28.143979" + }, + "sorted_bam_name": { + "content": [ + "test.marked.bam" + ], + "timestamp": "2024-01-19T10:26:45.080116" } } \ No newline at end of file diff --git a/modules/nf-core/qualimap/bamqc/tests/main.nf.test b/modules/nf-core/qualimap/bamqc/tests/main.nf.test index 5447fe68..ba2260ca 100644 --- a/modules/nf-core/qualimap/bamqc/tests/main.nf.test +++ b/modules/nf-core/qualimap/bamqc/tests/main.nf.test @@ -8,7 +8,7 @@ nextflow_process { tag "qualimap" tag "qualimap/bamqc" - test("homo_sapiens [BAM]") { + test("homo_sapiens [bam]") { when { params { @@ -16,11 +16,13 @@ nextflow_process { } process { """ - input[0] = [ + gff = [] + + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) - ] - input[1] = [] + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + input[1] = gff """ } } @@ -29,10 +31,8 @@ nextflow_process { assertAll ( { assert process.success }, { assert path("${process.out.results[0][1]}/qualimapReport.html").exists() }, - { assert snapshot( - path("${process.out.results[0][1]}/genome_results.txt"), - process.out.versions) - .match() } + { assert snapshot(path("${process.out.results[0][1]}/genome_results.txt")).match("genome_results") }, + { assert snapshot(process.out.versions).match("versions") } ) } } diff --git a/modules/nf-core/qualimap/bamqc/tests/main.nf.test.snap b/modules/nf-core/qualimap/bamqc/tests/main.nf.test.snap index 782f7d23..25148df2 100644 --- a/modules/nf-core/qualimap/bamqc/tests/main.nf.test.snap +++ b/modules/nf-core/qualimap/bamqc/tests/main.nf.test.snap @@ -1,11 +1,16 @@ { - "homo_sapiens [BAM]": { + "genome_results": { + "content": [ + "genome_results.txt:md5,45103d63ba82df2b905eb04819c32dd3" + ], + "timestamp": "2024-01-19T12:05:00.122103" + }, + "versions": { "content": [ - "genome_results.txt:md5,45103d63ba82df2b905eb04819c32dd3", [ "versions.yml:md5,9024d7d0a189d8be1485249ae591b907" ] ], - "timestamp": "2024-01-11T14:36:07.836508" + "timestamp": "2024-01-19T12:05:00.131485" } -} \ No newline at end of file +} diff --git a/modules/nf-core/samtools/stats/environment.yml b/modules/nf-core/samtools/stats/environment.yml index b89ce647..b45ba90c 100644 --- a/modules/nf-core/samtools/stats/environment.yml +++ b/modules/nf-core/samtools/stats/environment.yml @@ -5,3 +5,4 @@ channels: - defaults dependencies: - bioconda::samtools=1.18 + - bioconda::htslib=1.18 From ff858d717b98f1a9beee96b7b3abba716a2e9427 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 1 Feb 2024 17:06:20 +0100 Subject: [PATCH 302/387] update chromograph --- modules.json | 2 +- modules/nf-core/chromograph/main.nf | 4 +- modules/nf-core/chromograph/meta.yml | 2 +- .../nf-core/chromograph/tests/main.nf.test | 38 +++++++++++++++++++ modules/nf-core/chromograph/tests/tags.yml | 2 + 5 files changed, 44 insertions(+), 4 deletions(-) create mode 100644 modules/nf-core/chromograph/tests/main.nf.test create mode 100644 modules/nf-core/chromograph/tests/tags.yml diff --git a/modules.json b/modules.json index ff937852..522fd84f 100644 --- a/modules.json +++ b/modules.json @@ -82,7 +82,7 @@ }, "chromograph": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "f05512229a501df5d67273bb7358c5f69667f40d", "installed_by": ["modules"] }, "cnvnator/cnvnator": { diff --git a/modules/nf-core/chromograph/main.nf b/modules/nf-core/chromograph/main.nf index dd67e1ba..e1374e48 100644 --- a/modules/nf-core/chromograph/main.nf +++ b/modules/nf-core/chromograph/main.nf @@ -4,8 +4,8 @@ process CHROMOGRAPH { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/chromograph:1.3.1--pyhdfd78af_1': - 'biocontainers/chromograph:1.3.1--pyhdfd78af_1' }" + 'https://depot.galaxyproject.org/singularity/chromograph:1.3.1--pyhdfd78af_2': + 'biocontainers/chromograph:1.3.1--pyhdfd78af_2' }" input: tuple val(meta), path(autozyg) diff --git a/modules/nf-core/chromograph/meta.yml b/modules/nf-core/chromograph/meta.yml index 0fd7250f..6540d9f0 100644 --- a/modules/nf-core/chromograph/meta.yml +++ b/modules/nf-core/chromograph/meta.yml @@ -12,7 +12,7 @@ tools: description: "Chromograph is a python package to create PNG images from genetics data such as BED and WIG files." homepage: "https://github.com/Clinical-Genomics/chromograph" documentation: "https://github.com/Clinical-Genomics/chromograph/blob/master/README.md" - licence: "['MIT']" + licence: ["MIT"] input: - meta: type: map diff --git a/modules/nf-core/chromograph/tests/main.nf.test b/modules/nf-core/chromograph/tests/main.nf.test new file mode 100644 index 00000000..caba8829 --- /dev/null +++ b/modules/nf-core/chromograph/tests/main.nf.test @@ -0,0 +1,38 @@ +nextflow_process { + + name "Test Process CHROMOGRAPH" + script "modules/nf-core/chromograph/main.nf" + process "CHROMOGRAPH" + tag "modules" + tag "modules_nfcore" + tag "chromograph" + + test("test_chromograph_sites") { + + when { + process { + """ + input[0] = [[:],[]] + input[1] = [[:],[]] + input[2] = [[:],[]] + input[3] = [[:],[]] + input[4] = [[:],[]] + input[5] = [[:],[]] + input[6] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['genome']['updsites_bed'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert process.out.plots.get(0).get(1) ==~ ".*/test"} + ) + } + + } + +} diff --git a/modules/nf-core/chromograph/tests/tags.yml b/modules/nf-core/chromograph/tests/tags.yml new file mode 100644 index 00000000..e60ad9db --- /dev/null +++ b/modules/nf-core/chromograph/tests/tags.yml @@ -0,0 +1,2 @@ +chromograph: + - "modules/nf-core/chromograph/**" From f814060f4f21761d58909d99a59fa6b20908efca Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 02:55:26 +0100 Subject: [PATCH 303/387] add subsample --- conf/modules/subsample_mt.config | 21 ++++++++++- modules/local/calculate_seed_fraction.nf | 45 ++++++++++++++++++++++++ subworkflows/local/subsample_mt.nf | 26 +++++++++++--- workflows/raredisease.nf | 3 +- 4 files changed, 89 insertions(+), 6 deletions(-) create mode 100644 modules/local/calculate_seed_fraction.nf diff --git a/conf/modules/subsample_mt.config b/conf/modules/subsample_mt.config index 079affe9..7ce80bed 100644 --- a/conf/modules/subsample_mt.config +++ b/conf/modules/subsample_mt.config @@ -16,8 +16,27 @@ // process { - withName: '.*BEDTOOLS_GENOMECOV' { + withName: '.*SUBSAMPLE_MT:BEDTOOLS_GENOMECOV' { ext.args = { "-dz" } ext.prefix = { "${meta.id}" } } + + withName: '.*SUBSAMPLE_MT:SAMTOOLS_VIEW' { + ext.args = { "--output-fmt BAM -h -F 4 -s ${meta.seedfrac}" } + ext.prefix = { "${meta.id}_mt_subsample" } + publishDir = [ + path: { "${params.outdir}/alignment" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*SUBSAMPLE_MT:SAMTOOLS_INDEX' { + publishDir = [ + path: { "${params.outdir}/alignment" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + } diff --git a/modules/local/calculate_seed_fraction.nf b/modules/local/calculate_seed_fraction.nf new file mode 100644 index 00000000..6d776e14 --- /dev/null +++ b/modules/local/calculate_seed_fraction.nf @@ -0,0 +1,45 @@ +process CALCULATE_SEED_FRACTION { + tag "$meta.id" + label 'process_low' + + conda "conda-forge::python=3.8.3" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/python:3.8.3' : + 'biocontainers/python:3.8.3' }" + + input: + tuple val(meta), path(cov) + + output: + tuple val(meta), path("seedfrac.csv"), emit: csv + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + export MT_COVERAGE=`awk '{cov += \$3}END{ if (NR > 0) print cov / NR }' $cov` + + python -c "import os;print('%0.6f' % (30+ 150/float(os.environ['MT_COVERAGE'])))" >seedfrac.csv + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + calculate_seed_fraction: v1.0 + python: \$(python --version | sed 's/Python //g') + END_VERSIONS + """ + + stub: + """ + touch seedfrac.csv + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + calculate_seed_fraction: v1.0 + python: \$(python --version | sed 's/Python //g') + END_VERSIONS + """ +} diff --git a/subworkflows/local/subsample_mt.nf b/subworkflows/local/subsample_mt.nf index 6ffe8c98..b8d3ff2f 100644 --- a/subworkflows/local/subsample_mt.nf +++ b/subworkflows/local/subsample_mt.nf @@ -2,20 +2,38 @@ // A subworkflow to subsample MT alignments // -include { BEDTOOLS_GENOMECOV } from '../../modules/nf-core/bedtools/genomecov/main' +include { BEDTOOLS_GENOMECOV } from '../../modules/nf-core/bedtools/genomecov/main' +include { CALCULATE_SEED_FRACTION } from '../../modules/local/calculate_seed_fraction' +include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' workflow SUBSAMPLE_MT { take: - ch_mt_marked_bam // channel: [mandatory] [ val(meta), path(vcf), path(tbi) ] + ch_mt_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] main: + ch_versions = Channel.empty() - ch_mt_marked_bam.map {meta, bam -> return [meta, bam, []]}.set {ch_genomecov_in} + ch_mt_bam_bai.map {meta, bam, bai -> return [meta, bam, -1]}.set {ch_genomecov_in} - BEDTOOLS_GENOMECOV (ch_genomecov_in, [], []) + BEDTOOLS_GENOMECOV (ch_genomecov_in, [], "genomecov") + + CALCULATE_SEED_FRACTION (BEDTOOLS_GENOMECOV.out.genomecov).csv + .join(ch_mt_bam_bai, failOnMismatch:true) + .map{meta, seedfrac, bam, bai -> + return [meta + [seedfrac: file(seedfrac).text.readLines()[0]], bam, bai] + } + .set { ch_subsample_in } + + SAMTOOLS_VIEW(ch_subsample_in, [[:],[]], []) + + SAMTOOLS_INDEX(SAMTOOLS_VIEW.out.bam) ch_versions = ch_versions.mix(BEDTOOLS_GENOMECOV.out.versions.first()) + ch_versions = ch_versions.mix(CALCULATE_SEED_FRACTION.out.versions.first()) + ch_versions = ch_versions.mix(SAMTOOLS_VIEW.out.versions.first()) + ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) emit: versions = ch_versions // channel: [ path(versions.yml) ] diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 6884787d..eebb7886 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -363,8 +363,9 @@ workflow RAREDISEASE { .set { ch_mapped } ch_versions = ch_versions.mix(ALIGN.out.versions) - SUBSAMPLE_MT(ch_mapped.mt_marked_bam) + SUBSAMPLE_MT(ch_mapped.mt_bam_bai) + return // // BAM QUALITY CHECK // From c05325a0d2d3f35db254a5f178e966839be5b312 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 02:55:54 +0100 Subject: [PATCH 304/387] fix return --- workflows/raredisease.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index eebb7886..ed9f62a5 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -365,7 +365,6 @@ workflow RAREDISEASE { SUBSAMPLE_MT(ch_mapped.mt_bam_bai) - return // // BAM QUALITY CHECK // From 4aa778dd2d28a8e188bc47623369358745802343 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 10:56:02 +0100 Subject: [PATCH 305/387] add params --- docs/output.md | 13 ++++++++++++ modules/local/calculate_seed_fraction.nf | 4 +++- nextflow.config | 3 +++ nextflow_schema.json | 25 ++++++++++++++++++++++-- subworkflows/local/subsample_mt.nf | 11 +++++++++-- workflows/raredisease.nf | 9 ++++++++- 6 files changed, 59 insertions(+), 6 deletions(-) diff --git a/docs/output.md b/docs/output.md index 6cc3ecf9..90ac6f51 100644 --- a/docs/output.md +++ b/docs/output.md @@ -21,6 +21,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Duplicate marking](#duplicate-marking) - [Picard's MarkDuplicates](#picards-markduplicates) - [Sentieon Dedup](#sentieon-dedup) + - [Subsample mitochondrial alignments](#subsample-mitochondrial-alignments) - [Quality control and reporting](#quality-control-and-reporting) - [Quality control](#quality-control) - [FastQC](#fastqc) @@ -115,6 +116,18 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - `*.metrics`: Text file containing the dedup metrics. +#### Subsample mitochondrial alignments + +[Samtools view](https://www.htslib.org/doc/samtools-view.html) is used by the pipeline to subsample mitochondrial alignments to a user specified coverage. + +
+Output files from Alignment + +- `{outputdir}/alignment/` + - `_mt_subsample.bam`: Alignment file in bam format. + - `_mt_subsample.bam.bai`: Index of the corresponding bam file. +
+ ### Quality control and reporting #### Quality control diff --git a/modules/local/calculate_seed_fraction.nf b/modules/local/calculate_seed_fraction.nf index 6d776e14..1c367b38 100644 --- a/modules/local/calculate_seed_fraction.nf +++ b/modules/local/calculate_seed_fraction.nf @@ -9,6 +9,8 @@ process CALCULATE_SEED_FRACTION { input: tuple val(meta), path(cov) + val rd + val seed output: tuple val(meta), path("seedfrac.csv"), emit: csv @@ -23,7 +25,7 @@ process CALCULATE_SEED_FRACTION { """ export MT_COVERAGE=`awk '{cov += \$3}END{ if (NR > 0) print cov / NR }' $cov` - python -c "import os;print('%0.6f' % (30+ 150/float(os.environ['MT_COVERAGE'])))" >seedfrac.csv + python -c "import os;print('%0.6f' % ($seed+ $rd/float(os.environ['MT_COVERAGE'])))" >seedfrac.csv cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/nextflow.config b/nextflow.config index a18da1fb..69dd2cae 100644 --- a/nextflow.config +++ b/nextflow.config @@ -35,6 +35,7 @@ params { skip_sv_annotation = false skip_me_annotation = false skip_mt_annotation = false + skip_mt_subsample = false skip_vcf2cytosure = true skip_vep_filter = false gens_switch = false @@ -53,6 +54,8 @@ params { // Alignment aligner = 'bwamem2' min_trimmed_length = 40 + mt_subsample_rd = 150 + mt_subsample_seed = 30 rmdup = false // Variant calling diff --git a/nextflow_schema.json b/nextflow_schema.json index 74b35fd8..79045f6d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -27,7 +27,8 @@ "type": "string", "format": "directory-path", "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", - "fa_icon": "fas fa-folder-open" + "fa_icon": "fas fa-folder-open", + "default": "results" }, "email": { "type": "string", @@ -212,7 +213,8 @@ "type": "string", "description": "Name of the mitochondrial contig in the reference fasta file", "help_text": "Used to extract relevant information from the references to analyse mitochondria", - "fa_icon": "fas fa-align-center" + "fa_icon": "fas fa-align-center", + "default": "chrM" }, "mobile_element_references": { "type": "string", @@ -505,6 +507,11 @@ "description": "Specifies whether or not to skip annotation of mitochondrial variants.", "fa_icon": "fas fa-toggle-on" }, + "skip_mt_subsample": { + "type": "boolean", + "description": "Specifies whether or not to subsample mt alignment.", + "fa_icon": "fas fa-toggle-on" + }, "skip_snv_annotation": { "type": "boolean", "description": "Specifies whether or not to skip annotate SNV subworkflow.", @@ -549,6 +556,20 @@ "help_text": "Minimum length of reads after adapter trimming. Shorter reads are discarded. The program default is 15 bp. ", "fa_icon": "fas fa-less-than" }, + "mt_subsample_rd": { + "type": "integer", + "default": 150, + "description": "Expected coverage to subsample mt alignment to.", + "help_text": "To know more about this parameter check samtools' view documentation.", + "fa_icon": "fas fa-less-than" + }, + "mt_subsample_seed": { + "type": "integer", + "default": 30, + "description": "Subsampling seed used to influence which subset of mitochondrial reads is kept. ", + "help_text": "To know more about this parameter check samtools' view documentation.", + "fa_icon": "fas fa-less-than" + }, "rmdup": { "type": "boolean", "description": "Specifies whether duplicates reads should be removed prior to variant calling.", diff --git a/subworkflows/local/subsample_mt.nf b/subworkflows/local/subsample_mt.nf index b8d3ff2f..dd6b87b0 100644 --- a/subworkflows/local/subsample_mt.nf +++ b/subworkflows/local/subsample_mt.nf @@ -10,7 +10,9 @@ include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/m workflow SUBSAMPLE_MT { take: - ch_mt_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_mt_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + val_mt_subsample_rd // channel: [mandatory] [ val(read_dept) ] + val_mt_subsample_seed // channel: [mandatory] [ val(seed) ] main: ch_versions = Channel.empty() @@ -19,7 +21,12 @@ workflow SUBSAMPLE_MT { BEDTOOLS_GENOMECOV (ch_genomecov_in, [], "genomecov") - CALCULATE_SEED_FRACTION (BEDTOOLS_GENOMECOV.out.genomecov).csv + CALCULATE_SEED_FRACTION ( + BEDTOOLS_GENOMECOV.out.genomecov, + val_mt_subsample_rd, + val_mt_subsample_seed + ) + .csv .join(ch_mt_bam_bai, failOnMismatch:true) .map{meta, seedfrac, bam, bai -> return [meta + [seedfrac: file(seedfrac).text.readLines()[0]], bam, bai] diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index ed9f62a5..01a9bb88 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -363,7 +363,14 @@ workflow RAREDISEASE { .set { ch_mapped } ch_versions = ch_versions.mix(ALIGN.out.versions) - SUBSAMPLE_MT(ch_mapped.mt_bam_bai) + if (params.skip_mt_subsample) { + SUBSAMPLE_MT( + ch_mapped.mt_bam_bai, + params.mt_subsample_rd, + params.mt_subsample_seed + ) + ch_versions = ch_versions.mix(SUBSAMPLE_MT.out.versions) + } // // BAM QUALITY CHECK From 05be462f9a4e2172563e162c6f4d6ede1f208e71 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 11:00:44 +0100 Subject: [PATCH 306/387] update conditional --- workflows/raredisease.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 01a9bb88..650ca446 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -363,7 +363,7 @@ workflow RAREDISEASE { .set { ch_mapped } ch_versions = ch_versions.mix(ALIGN.out.versions) - if (params.skip_mt_subsample) { + if (!params.skip_mt_subsample) { SUBSAMPLE_MT( ch_mapped.mt_bam_bai, params.mt_subsample_rd, From a722bc194df30865053a1824d8f3b153feb76dd9 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 11:04:59 +0100 Subject: [PATCH 307/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 55ea2691..17fc2667 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -25,6 +25,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Add FOUND_IN tag, which mentions the variant caller that found the mutation, in the INFO column of the vcf files [#471](https://github.com/nf-core/raredisease/pull/471) - A new parameter `vep_plugin_files` to supply files required by vep plugins [#482](https://github.com/nf-core/raredisease/pull/482) - New workflow for annotating mobile elements [#483](https://github.com/nf-core/raredisease/pull/483) +- Added a functionality to subsample mitochondrial alignment, and a new parameter `skip_mt_subsample` to skip the subworkflow [#508](https://github.com/nf-core/raredisease/pull/508). ### `Changed` From 1f4368dbe5eb7fdc9cb79a125e88d3c7cbc49dd1 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 11:06:56 +0100 Subject: [PATCH 308/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 55ea2691..1059ff5d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -25,6 +25,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Add FOUND_IN tag, which mentions the variant caller that found the mutation, in the INFO column of the vcf files [#471](https://github.com/nf-core/raredisease/pull/471) - A new parameter `vep_plugin_files` to supply files required by vep plugins [#482](https://github.com/nf-core/raredisease/pull/482) - New workflow for annotating mobile elements [#483](https://github.com/nf-core/raredisease/pull/483) +- Chromograph to plot coverage across chromosomes [#507](https://github.com/nf-core/raredisease/pull/507) ### `Changed` From b1f0c847322155d57906a6cb80b900729926a068 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 14:16:50 +0100 Subject: [PATCH 309/387] update output docs --- docs/output.md | 11 +++++++++++ 1 file changed, 11 insertions(+) diff --git a/docs/output.md b/docs/output.md index 6cc3ecf9..f287ce08 100644 --- a/docs/output.md +++ b/docs/output.md @@ -27,6 +27,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Mosdepth](#mosdepth) - [Picard tools](#picard-tools) - [Qualimap](#qualimap) + - [Chromograph coverage](#chromograph-coverage) - [Sention WgsMetricsAlgo](#sention-wgsmetricsalgo) - [TIDDIT's cov and UCSC WigToBigWig](#tiddits-cov-and-ucsc-wigtobigwig) - [Reporting](#reporting) @@ -183,6 +184,16 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - `{outputdir}/qc_bam/_qualimap/` this directory includes a qualimap report and associated raw statistic files. You can open the .html file in your internet browser to see the in-depth report. +##### Chromograph coverage + +[Chromograph](https://github.com/Clinical-Genomics/chromograph) is a python package to create PNG images from genetics data such as BED and WIG files. + +
+Output files + +- `{outputdir}/qc_bam/_chromographcov/*.png` plots showing coverage across chromosomes for each chromosome. +
+ ##### Sention WgsMetricsAlgo [Sentieon's WgsMetricsAlgo](https://support.sentieon.com/manual/usages/general/) is the Sentieon's equivalent of Picard's CollectWgsMetrics. From 0273a3757728a55791198729f2b46ac8368d4183 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 31 Jan 2024 21:45:56 +0100 Subject: [PATCH 310/387] chromograph viz --- conf/modules/qc_bam.config | 5 +++++ subworkflows/local/qc_bam.nf | 5 ++++- 2 files changed, 9 insertions(+), 1 deletion(-) diff --git a/conf/modules/qc_bam.config b/conf/modules/qc_bam.config index 61daf4d6..93b0f2db 100644 --- a/conf/modules/qc_bam.config +++ b/conf/modules/qc_bam.config @@ -50,6 +50,11 @@ process { ext.args = '-clip' } + withName: '.*QC_BAM:CHROMOGRAPH_COV' { + ext.args = '--euploid --step 500' + ext.prefix = { "${meta2.id}_chromographcov" } + } + withName: '.*QC_BAM:MOSDEPTH' { ext.args = '--d4' ext.prefix = { "${meta.id}_mosdepth" } diff --git a/subworkflows/local/qc_bam.nf b/subworkflows/local/qc_bam.nf index d4ae2842..9bb5a2a4 100644 --- a/subworkflows/local/qc_bam.nf +++ b/subworkflows/local/qc_bam.nf @@ -4,13 +4,14 @@ include { PICARD_COLLECTMULTIPLEMETRICS } from '../../modules/nf-core/picard/collectmultiplemetrics/main' include { PICARD_COLLECTHSMETRICS } from '../../modules/nf-core/picard/collecthsmetrics/main' +include { CHROMOGRAPH as CHROMOGRAPH_COV } from '../../modules/nf-core/chromograph/main' include { QUALIMAP_BAMQC } from '../../modules/nf-core/qualimap/bamqc/main' include { TIDDIT_COV } from '../../modules/nf-core/tiddit/cov/main' include { MOSDEPTH } from '../../modules/nf-core/mosdepth/main' include { UCSC_WIGTOBIGWIG } from '../../modules/nf-core/ucsc/wigtobigwig/main' include { PICARD_COLLECTWGSMETRICS as PICARD_COLLECTWGSMETRICS } from '../../modules/nf-core/picard/collectwgsmetrics/main' include { PICARD_COLLECTWGSMETRICS as PICARD_COLLECTWGSMETRICS_Y } from '../../modules/nf-core/picard/collectwgsmetrics/main' -include { SENTIEON_WGSMETRICS } from '../../modules/nf-core/sentieon/wgsmetrics/main' +include { SENTIEON_WGSMETRICS } from '../../modules/nf-core/sentieon/wgsmetrics/main' include { SENTIEON_WGSMETRICS as SENTIEON_WGSMETRICS_Y } from '../../modules/nf-core/sentieon/wgsmetrics/main' include { NGSBITS_SAMPLEGENDER } from '../../modules/nf-core/ngsbits/samplegender/main' @@ -51,6 +52,8 @@ workflow QC_BAM { UCSC_WIGTOBIGWIG (TIDDIT_COV.out.wig, ch_chrom_sizes) + CHROMOGRAPH_COV([[:],[]], TIDDIT_COV.out.wig, [[:],[]], [[:],[]], [[:],[]], [[:],[]], [[:],[]]) + ch_bam_bai.map{ meta, bam, bai -> [meta, bam, bai, []]}.set{ch_mosdepth_in} MOSDEPTH (ch_mosdepth_in, ch_genome_fasta) From 8e39cb8e2c0fc27a5356b6760604ae3afea3a90d Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 1 Feb 2024 17:06:20 +0100 Subject: [PATCH 311/387] update chromograph --- modules.json | 2 +- modules/nf-core/chromograph/main.nf | 4 +- modules/nf-core/chromograph/meta.yml | 2 +- .../nf-core/chromograph/tests/main.nf.test | 38 +++++++++++++++++++ modules/nf-core/chromograph/tests/tags.yml | 2 + 5 files changed, 44 insertions(+), 4 deletions(-) create mode 100644 modules/nf-core/chromograph/tests/main.nf.test create mode 100644 modules/nf-core/chromograph/tests/tags.yml diff --git a/modules.json b/modules.json index 2b008539..ffdf8a5f 100644 --- a/modules.json +++ b/modules.json @@ -87,7 +87,7 @@ }, "chromograph": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "f05512229a501df5d67273bb7358c5f69667f40d", "installed_by": ["modules"] }, "cnvnator/cnvnator": { diff --git a/modules/nf-core/chromograph/main.nf b/modules/nf-core/chromograph/main.nf index dd67e1ba..e1374e48 100644 --- a/modules/nf-core/chromograph/main.nf +++ b/modules/nf-core/chromograph/main.nf @@ -4,8 +4,8 @@ process CHROMOGRAPH { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/chromograph:1.3.1--pyhdfd78af_1': - 'biocontainers/chromograph:1.3.1--pyhdfd78af_1' }" + 'https://depot.galaxyproject.org/singularity/chromograph:1.3.1--pyhdfd78af_2': + 'biocontainers/chromograph:1.3.1--pyhdfd78af_2' }" input: tuple val(meta), path(autozyg) diff --git a/modules/nf-core/chromograph/meta.yml b/modules/nf-core/chromograph/meta.yml index 0fd7250f..6540d9f0 100644 --- a/modules/nf-core/chromograph/meta.yml +++ b/modules/nf-core/chromograph/meta.yml @@ -12,7 +12,7 @@ tools: description: "Chromograph is a python package to create PNG images from genetics data such as BED and WIG files." homepage: "https://github.com/Clinical-Genomics/chromograph" documentation: "https://github.com/Clinical-Genomics/chromograph/blob/master/README.md" - licence: "['MIT']" + licence: ["MIT"] input: - meta: type: map diff --git a/modules/nf-core/chromograph/tests/main.nf.test b/modules/nf-core/chromograph/tests/main.nf.test new file mode 100644 index 00000000..caba8829 --- /dev/null +++ b/modules/nf-core/chromograph/tests/main.nf.test @@ -0,0 +1,38 @@ +nextflow_process { + + name "Test Process CHROMOGRAPH" + script "modules/nf-core/chromograph/main.nf" + process "CHROMOGRAPH" + tag "modules" + tag "modules_nfcore" + tag "chromograph" + + test("test_chromograph_sites") { + + when { + process { + """ + input[0] = [[:],[]] + input[1] = [[:],[]] + input[2] = [[:],[]] + input[3] = [[:],[]] + input[4] = [[:],[]] + input[5] = [[:],[]] + input[6] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['genome']['updsites_bed'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert process.out.plots.get(0).get(1) ==~ ".*/test"} + ) + } + + } + +} diff --git a/modules/nf-core/chromograph/tests/tags.yml b/modules/nf-core/chromograph/tests/tags.yml new file mode 100644 index 00000000..e60ad9db --- /dev/null +++ b/modules/nf-core/chromograph/tests/tags.yml @@ -0,0 +1,2 @@ +chromograph: + - "modules/nf-core/chromograph/**" From c18ee8ef42c37eaa1496c4ea06130102974c8b73 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 11:06:56 +0100 Subject: [PATCH 312/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 17fc2667..db07d7eb 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -26,6 +26,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - A new parameter `vep_plugin_files` to supply files required by vep plugins [#482](https://github.com/nf-core/raredisease/pull/482) - New workflow for annotating mobile elements [#483](https://github.com/nf-core/raredisease/pull/483) - Added a functionality to subsample mitochondrial alignment, and a new parameter `skip_mt_subsample` to skip the subworkflow [#508](https://github.com/nf-core/raredisease/pull/508). +- Chromograph to plot coverage across chromosomes [#507](https://github.com/nf-core/raredisease/pull/507) ### `Changed` From e50e30c52fc5ea7f250d6d960b19cf8ce2a69258 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 14:16:50 +0100 Subject: [PATCH 313/387] update output docs --- docs/output.md | 11 +++++++++++ 1 file changed, 11 insertions(+) diff --git a/docs/output.md b/docs/output.md index 90ac6f51..7004fc1a 100644 --- a/docs/output.md +++ b/docs/output.md @@ -28,6 +28,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Mosdepth](#mosdepth) - [Picard tools](#picard-tools) - [Qualimap](#qualimap) + - [Chromograph coverage](#chromograph-coverage) - [Sention WgsMetricsAlgo](#sention-wgsmetricsalgo) - [TIDDIT's cov and UCSC WigToBigWig](#tiddits-cov-and-ucsc-wigtobigwig) - [Reporting](#reporting) @@ -196,6 +197,16 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - `{outputdir}/qc_bam/_qualimap/` this directory includes a qualimap report and associated raw statistic files. You can open the .html file in your internet browser to see the in-depth report. +##### Chromograph coverage + +[Chromograph](https://github.com/Clinical-Genomics/chromograph) is a python package to create PNG images from genetics data such as BED and WIG files. + +
+Output files + +- `{outputdir}/qc_bam/_chromographcov/*.png` plots showing coverage across chromosomes for each chromosome. +
+ ##### Sention WgsMetricsAlgo [Sentieon's WgsMetricsAlgo](https://support.sentieon.com/manual/usages/general/) is the Sentieon's equivalent of Picard's CollectWgsMetrics. From a9c3d9cbe2d57972651f5bae2dfc7b8b47933e7c Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 11:04:59 +0100 Subject: [PATCH 314/387] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index db07d7eb..d5135e7c 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -27,6 +27,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New workflow for annotating mobile elements [#483](https://github.com/nf-core/raredisease/pull/483) - Added a functionality to subsample mitochondrial alignment, and a new parameter `skip_mt_subsample` to skip the subworkflow [#508](https://github.com/nf-core/raredisease/pull/508). - Chromograph to plot coverage across chromosomes [#507](https://github.com/nf-core/raredisease/pull/507) +- Added a functionality to subsample mitochondrial alignment, and a new parameter `skip_mt_subsample` to skip the subworkflow [#508](https://github.com/nf-core/raredisease/pull/508). ### `Changed` From fca0ac638b1f8c4aed3b84614ced7521965e14e2 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 15:49:03 +0100 Subject: [PATCH 315/387] review suggestions --- docs/output.md | 2 +- subworkflows/local/subsample_mt.nf | 14 +++++++------- 2 files changed, 8 insertions(+), 8 deletions(-) diff --git a/docs/output.md b/docs/output.md index 7004fc1a..e6e231e2 100644 --- a/docs/output.md +++ b/docs/output.md @@ -119,7 +119,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d #### Subsample mitochondrial alignments -[Samtools view](https://www.htslib.org/doc/samtools-view.html) is used by the pipeline to subsample mitochondrial alignments to a user specified coverage. +[Samtools view](https://www.htslib.org/doc/samtools-view.html) is used by the pipeline to subsample mitochondrial alignments to a user specified coverage. The file is mainly intended to be used for visualization of MT alignments in IGV. The non-subsampled bam file is used for variant calling and other downstream analysis steps.
Output files from Alignment diff --git a/subworkflows/local/subsample_mt.nf b/subworkflows/local/subsample_mt.nf index dd6b87b0..d8da5aae 100644 --- a/subworkflows/local/subsample_mt.nf +++ b/subworkflows/local/subsample_mt.nf @@ -25,13 +25,13 @@ workflow SUBSAMPLE_MT { BEDTOOLS_GENOMECOV.out.genomecov, val_mt_subsample_rd, val_mt_subsample_seed - ) - .csv - .join(ch_mt_bam_bai, failOnMismatch:true) - .map{meta, seedfrac, bam, bai -> - return [meta + [seedfrac: file(seedfrac).text.readLines()[0]], bam, bai] - } - .set { ch_subsample_in } + ) + .csv + .join(ch_mt_bam_bai, failOnMismatch:true) + .map{meta, seedfrac, bam, bai -> + return [meta + [seedfrac: file(seedfrac).text.readLines()[0]], bam, bai] + } + .set { ch_subsample_in } SAMTOOLS_VIEW(ch_subsample_in, [[:],[]], []) From cf6c313a9231c3c9695b443cf098d0ec6f5b9e52 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 15:57:52 +0100 Subject: [PATCH 316/387] fix lint error --- docs/output.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/output.md b/docs/output.md index e6e231e2..37f7d4f8 100644 --- a/docs/output.md +++ b/docs/output.md @@ -119,7 +119,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d #### Subsample mitochondrial alignments -[Samtools view](https://www.htslib.org/doc/samtools-view.html) is used by the pipeline to subsample mitochondrial alignments to a user specified coverage. The file is mainly intended to be used for visualization of MT alignments in IGV. The non-subsampled bam file is used for variant calling and other downstream analysis steps. +[Samtools view](https://www.htslib.org/doc/samtools-view.html) is used by the pipeline to subsample mitochondrial alignments to a user specified coverage. The file is mainly intended to be used for visualization of MT alignments in IGV. The non-subsampled bam file is used for variant calling and other downstream analysis steps.
Output files from Alignment From 73729cfbb8a14b09e95cff2dddb8122011439938 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 21:03:23 +0100 Subject: [PATCH 317/387] add two params --- assets/variant_consequences_v2.txt | 41 ------------------ conf/test.config | 4 +- conf/test_one_sample.config | 4 +- main.nf | 2 + nextflow_schema.json | 69 +++++++++++++++++++++++++----- workflows/raredisease.nf | 21 +++++---- 6 files changed, 79 insertions(+), 62 deletions(-) delete mode 100644 assets/variant_consequences_v2.txt diff --git a/assets/variant_consequences_v2.txt b/assets/variant_consequences_v2.txt deleted file mode 100644 index effe32b1..00000000 --- a/assets/variant_consequences_v2.txt +++ /dev/null @@ -1,41 +0,0 @@ -transcript_ablation -splice_acceptor_variant -splice_donor_variant -stop_gained -frameshift_variant -stop_lost -start_lost -transcript_amplification -feature_elongation -feature_truncation -inframe_insertion -inframe_deletion -missense_variant -protein_altering_variant -splice_donor_5th_base_variant -splice_region_variant -splice_donor_region_variant -splice_polypyrimidine_tract_variant -incomplete_terminal_codon_variant -start_retained_variant -stop_retained_variant -synonymous_variant -coding_sequence_variant -mature_miRNA_variant -5_prime_UTR_variant -3_prime_UTR_variant -non_coding_transcript_exon_variant -intron_variant -NMD_transcript_variant -non_coding_transcript_variant -coding_transcript_variant -upstream_gene_variant -downstream_gene_variant -TFBS_ablation -TFBS_amplification -TF_binding_site_variant -regulatory_region_ablation -regulatory_region_amplification -regulatory_region_variant -intergenic_variant -sequence_variant diff --git a/conf/test.config b/conf/test.config index fd2f873e..75e7a92f 100644 --- a/conf/test.config +++ b/conf/test.config @@ -43,7 +43,7 @@ params { intervals_y = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/targetY.interval_list" known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" - mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" + mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" mobile_element_svdb_annotations = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/svdb_querydb_files.csv" reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" score_config_mt = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" @@ -55,6 +55,8 @@ params { vcfanno_lua = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_functions.lua" vcfanno_resources = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_resources.txt" vcfanno_toml = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_config.toml" + variant_consequences_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/variant_consequences_v2.txt" + variant_consequences_sv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/variant_consequences_v2.txt" vep_cache = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_cache_and_plugins.tar.gz" vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" vep_cache_version = 107 diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index d521a8a3..f54448f8 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -43,7 +43,7 @@ params { intervals_y = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/targetY.interval_list" known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" - mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" + mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" mobile_element_svdb_annotations = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/svdb_querydb_files.csv" reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" score_config_mt = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" @@ -55,6 +55,8 @@ params { vcfanno_lua = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_functions.lua" vcfanno_resources = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_resources.txt" vcfanno_toml = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_config.toml" + variant_consequences_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/variant_consequences_v2.txt" + variant_consequences_sv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/variant_consequences_v2.txt" vep_cache = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_cache_and_plugins.tar.gz" vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" vep_cache_version = 107 diff --git a/main.nf b/main.nf index 72b342a3..6c38b158 100644 --- a/main.nf +++ b/main.nf @@ -47,6 +47,8 @@ params.sdf = WorkflowMain.getGenomeAttribute(params, params.svdb_query_dbs = WorkflowMain.getGenomeAttribute(params, 'svdb_query_dbs') params.target_bed = WorkflowMain.getGenomeAttribute(params, 'target_bed') params.variant_catalog = WorkflowMain.getGenomeAttribute(params, 'variant_catalog') +params.variant_consequences_snv = WorkflowMain.getGenomeAttribute(params, 'variant_consequences_snv') +params.variant_consequences_sv = WorkflowMain.getGenomeAttribute(params, 'variant_consequences_sv') params.vep_filters = WorkflowMain.getGenomeAttribute(params, 'vep_filters') params.vcf2cytosure_blacklist = WorkflowMain.getGenomeAttribute(params, 'vcf2cytosure_blacklist') params.vcfanno_resources = WorkflowMain.getGenomeAttribute(params, 'vcfanno_resources') diff --git a/nextflow_schema.json b/nextflow_schema.json index 79045f6d..47c4f0ae 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,7 +10,9 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["outdir"], + "required": [ + "outdir" + ], "properties": { "input": { "type": "string", @@ -423,7 +425,11 @@ "description": "Path to the file containing HGNC_IDs of interest on separate lines." } }, - "required": ["fasta", "intervals_wgs", "intervals_y"] + "required": [ + "fasta", + "intervals_wgs", + "intervals_y" + ] }, "analysis_options": { "title": "Analysis options", @@ -436,7 +442,11 @@ "default": "wgs", "description": "Specifies which analysis type for the pipeline- either 'wgs','wes','mito'. This changes resources consumed and tools used.", "fa_icon": "fas fa-align-center", - "enum": ["wgs", "wes", "mito"] + "enum": [ + "wgs", + "wes", + "mito" + ] }, "gens_switch": { "type": "boolean", @@ -448,14 +458,20 @@ "default": "illumina", "description": "Specifies which analysis type for the pipeline- either 'wgs','wes','mito'. This changes resources consumed and tools used.", "fa_icon": "fas fa-align-center", - "enum": ["illumina"] + "enum": [ + "illumina" + ] }, "ngsbits_samplegender_method": { "type": "string", "default": "xy", "description": "Method selection for ngs-bits samplegender", "fa_icon": "fas fa-align-center", - "enum": ["xy", "hetx", "sry"] + "enum": [ + "xy", + "hetx", + "sry" + ] }, "run_rtgvcfeval": { "type": "boolean", @@ -547,7 +563,11 @@ "default": "bwamem2", "description": "Specifies the alignment algorithm to use - available options are 'bwamem2' and 'sentieon'.", "fa_icon": "fas fa-align-center", - "enum": ["bwa", "bwamem2", "sentieon"] + "enum": [ + "bwa", + "bwamem2", + "sentieon" + ] }, "min_trimmed_length": { "type": "integer", @@ -608,14 +628,21 @@ "default": "deepvariant", "description": "Specifies the variant caller to use - available options are 'deepvariant' and 'sentieon'.", "fa_icon": "fas fa-random", - "enum": ["deepvariant", "sentieon"] + "enum": [ + "deepvariant", + "sentieon" + ] }, "variant_type": { "type": "string", "default": "snp,indel", "description": "Specifies the variant types for sentieon variant caller.", "fa_icon": "fas fa-align-center", - "enum": ["snp", "indel", "snp,indel"] + "enum": [ + "snp", + "indel", + "snp,indel" + ] } } }, @@ -625,12 +652,27 @@ "fa_icon": "fas fa-user-cog", "description": "Options used to facilitate the annotation of the variants.", "properties": { + "variant_consequences_snv": { + "type": "string", + "description": "File containing list of SO terms listed in the order of severity from most severe to lease severe for annotating genomic and mitochondrial SNVs.", + "help_text": "For more information check https://grch37.ensembl.org/info/genome/variation/prediction/predicted_data.html", + "fa_icon": "fas fa-file-csv" + }, + "variant_consequences_sv": { + "type": "string", + "description": "File containing list of SO terms listed in the order of severity from most severe to lease severe for annotating genomic SVs.", + "help_text": "For more information check https://grch37.ensembl.org/info/genome/variation/prediction/predicted_data.html", + "fa_icon": "fas fa-file-csv" + }, "vep_cache_version": { "type": "integer", "default": 110, "description": "Specify the version of the VEP cache provided to the `--vep_cache` option.", "fa_icon": "fas fa-align-center", - "enum": [107, 110] + "enum": [ + 107, + 110 + ] } } }, @@ -742,7 +784,14 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], "hidden": true }, "email_on_fail": { diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 650ca446..3d8984c5 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -40,11 +40,11 @@ if (params.run_rtgvcfeval) { if (!params.skip_snv_annotation) { mandatoryParams += ["genome", "vcfanno_resources", "vcfanno_toml", "vep_cache", "vep_cache_version", - "gnomad_af", "score_config_snv"] + "gnomad_af", "score_config_snv", "variant_consequences_snv"] } if (!params.skip_sv_annotation) { - mandatoryParams += ["genome", "vep_cache", "vep_cache_version", "score_config_sv"] + mandatoryParams += ["genome", "vep_cache", "vep_cache_version", "score_config_sv", "variant_consequences_sv"] if (!params.svdb_query_bedpedbs && !params.svdb_query_dbs) { println("params.svdb_query_bedpedbs or params.svdb_query_dbs should be set.") missingParamsCount += 1 @@ -52,7 +52,7 @@ if (!params.skip_sv_annotation) { } if (!params.skip_mt_annotation) { - mandatoryParams += ["genome", "mito_name", "vcfanno_resources", "vcfanno_toml", "vep_cache_version", "vep_cache"] + mandatoryParams += ["genome", "mito_name", "vcfanno_resources", "vcfanno_toml", "vep_cache_version", "vep_cache", "variant_consequences_snv"] } if (params.analysis_type.equals("wes")) { @@ -72,7 +72,7 @@ if (!params.skip_vep_filter) { } if (!params.skip_me_annotation) { - mandatoryParams += ["mobile_element_svdb_annotations"] + mandatoryParams += ["mobile_element_svdb_annotations", "variant_consequences_snv"] } for (param in mandatoryParams.unique()) { @@ -288,7 +288,10 @@ workflow RAREDISEASE { ch_target_intervals = ch_references.target_intervals ch_variant_catalog = params.variant_catalog ? Channel.fromPath(params.variant_catalog).map { it -> [[id:it[0].simpleName],it]}.collect() : Channel.value([[],[]]) - ch_variant_consequences = Channel.fromPath("$projectDir/assets/variant_consequences_v2.txt", checkIfExists: true).collect() + ch_variant_consequences_snv = params.variant_consequences_snv ? Channel.fromPath(params.variant_consequences_snv).collect() + : Channel.value([]) + ch_variant_consequences_sv = params.variant_consequences_sv ? Channel.fromPath(params.variant_consequences_sv).collect() + : Channel.value([]) ch_vcfanno_resources = params.vcfanno_resources ? Channel.fromPath(params.vcfanno_resources).splitText().map{it -> it.trim()}.collect() : Channel.value([]) ch_vcf2cytosure_blacklist = params.vcf2cytosure_blacklist ? Channel.fromPath(params.vcf2cytosure_blacklist).collect() @@ -490,7 +493,7 @@ workflow RAREDISEASE { ANN_CSQ_PLI_SV ( GENERATE_CLINICAL_SET_SV.out.vcf, - ch_variant_consequences + ch_variant_consequences_sv ) ch_versions = ch_versions.mix(ANN_CSQ_PLI_SV.out.versions) @@ -535,7 +538,7 @@ workflow RAREDISEASE { ANN_CSQ_PLI_SNV ( GENERATE_CLINICAL_SET_SNV.out.vcf, - ch_variant_consequences + ch_variant_consequences_snv ) ch_versions = ch_versions.mix(ANN_CSQ_PLI_SNV.out.versions) @@ -577,7 +580,7 @@ workflow RAREDISEASE { ANN_CSQ_PLI_MT( GENERATE_CLINICAL_SET_MT.out.vcf, - ch_variant_consequences + ch_variant_consequences_snv ) ch_versions = ch_versions.mix(ANN_CSQ_PLI_MT.out.versions) @@ -663,7 +666,7 @@ workflow RAREDISEASE { ch_genome_fasta, ch_genome_dictionary, ch_vep_cache, - ch_variant_consequences, + ch_variant_consequences_sv, ch_vep_filters, params.genome, params.vep_cache_version, From 1ea4ba1892c378266d895e9ec2ac653491ea3415 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 21:56:04 +0100 Subject: [PATCH 318/387] update changelog and docs --- CHANGELOG.md | 1 + docs/usage.md | 50 ++++++++++++++++++++++++++++---------------------- 2 files changed, 29 insertions(+), 22 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index db07d7eb..06a3851f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -27,6 +27,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New workflow for annotating mobile elements [#483](https://github.com/nf-core/raredisease/pull/483) - Added a functionality to subsample mitochondrial alignment, and a new parameter `skip_mt_subsample` to skip the subworkflow [#508](https://github.com/nf-core/raredisease/pull/508). - Chromograph to plot coverage across chromosomes [#507](https://github.com/nf-core/raredisease/pull/507) +- Added two new parameters `variant_consequences_snv` and `variant_consequences_sv` to supply variant consequence files for annotating SNVs and SVs. [#509](https://github.com/nf-core/raredisease/pull/509) ### `Changed` diff --git a/docs/usage.md b/docs/usage.md index 8f1ec601..f75baed9 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -221,15 +221,16 @@ The mandatory and optional parameters for each category are tabulated below. ##### 7. SNV annotation & Ranking -| Mandatory | Optional | -| ----------------------------- | ------------------------------ | -| genome1 | reduced_penetrance7 | -| vcfanno_resources2 | vcfanno_lua | -| vcfanno_toml3 | vep_filters8 | -| vep_cache_version | cadd_resources9 | -| vep_cache4 | vep_plugin_files10 | -| gnomad_af5 | | -| score_config_snv6 | | +| Mandatory | Optional | +| ------------------------------------ | ------------------------------ | +| genome1 | reduced_penetrance8 | +| vcfanno_resources2 | vcfanno_lua | +| vcfanno_toml3 | vep_filters9 | +| vep_cache_version | cadd_resources10 | +| vep_cache4 | vep_plugin_files11 | +| gnomad_af5 | | +| score_config_snv6 | | +| variant_consequences_snv7 | | 1Genome version is used by VEP. You have the option to choose between GRCh37 and GRCh38.
2Path to VCF files and their indices used by vcfanno. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/vcfanno_resources.txt).
@@ -240,10 +241,11 @@ See example cache [here](https://raw.githubusercontent.com/nf-core/test-datasets 5 GnomAD VCF files can be downloaded from [here](https://gnomad.broadinstitute.org/downloads). The option `gnomad_af` expects a tab-delimited file with no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/gnomad_reformated.tab.gz).
6Used by GENMOD for ranking the variants. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/rank_model_snv.ini).
-7Used by GENMOD while modeling the variants. Contains a list of loci that show [reduced penetrance](https://medlineplus.gov/genetics/understanding/inheritance/penetranceexpressivity/) in people. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/reduced_penetrance.tsv).
-8 This file contains a list of candidate genes (with [HGNC](https://www.genenames.org/) IDs) that is used to split the variants into canditate variants and research variants. Research variants contain all the variants, while candidate variants are a subset of research variants and are associated with candidate genes. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/hgnc.txt). Not required if --skip_vep_filter is set to true.
-9Path to a folder containing cadd annotations. Equivalent of the data/annotations/ folder described [here](https://github.com/kircherlab/CADD-scripts/#manual-installation), and it is used to calculate CADD scores for small indels.
-10A CSV file that describes the files used by VEP's named and custom plugins. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/vep_files.csv).
+7File containing list of SO terms listed in the order of severity from most severe to lease severe for annotating genomic and mitochondrial SNVs. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/variant_consequences_v2.txt). You can learn more about these terms [here](https://grch37.ensembl.org/info/genome/variation/prediction/predicted_data.html). +8Used by GENMOD while modeling the variants. Contains a list of loci that show [reduced penetrance](https://medlineplus.gov/genetics/understanding/inheritance/penetranceexpressivity/) in people. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/reduced_penetrance.tsv).
+9 This file contains a list of candidate genes (with [HGNC](https://www.genenames.org/) IDs) that is used to split the variants into canditate variants and research variants. Research variants contain all the variants, while candidate variants are a subset of research variants and are associated with candidate genes. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/hgnc.txt). Not required if --skip_vep_filter is set to true.
+10Path to a folder containing cadd annotations. Equivalent of the data/annotations/ folder described [here](https://github.com/kircherlab/CADD-scripts/#manual-installation), and it is used to calculate CADD scores for small indels.
+11A CSV file that describes the files used by VEP's named and custom plugins. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/vep_files.csv).
> NB: We use CADD only to annotate small indels. To annotate SNVs with precomputed CADD scores, pass the file containing CADD scores as a resource to vcfanno instead. Files containing the precomputed CADD scores for SNVs can be downloaded from [here](https://cadd.gs.washington.edu/download) (description: "All possible SNVs of GRCh3<7/8>/hg3<7/8>") @@ -256,20 +258,23 @@ no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sampl | vep_cache_version | vep_filters | | vep_cache | vep_plugin_files | | score_config_sv | | +| variant_consequences_sv2 | | 1 A CSV file that describes the databases (VCFs or BEDPEs) used by SVDB for annotating structural variants. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/svdb_querydb_files.csv). Information about the column headers can be found [here](https://github.com/J35P312/SVDB#Query). +2 File containing list of SO terms listed in the order of severity from most severe to lease severe for annotating genomic SVs. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/variant_consequences_v2.txt). You can learn more about these terms [here](https://grch37.ensembl.org/info/genome/variation/prediction/predicted_data.html). ##### 9. Mitochondrial annotation -| Mandatory | Optional | -| ----------------- | ---------------- | -| genome | vep_filters | -| mito_name | vep_plugin_files | -| vcfanno_resources | | -| vcfanno_toml | | -| vep_cache_version | | -| vep_cache | | -| score_config_mt | | +| Mandatory | Optional | +| ------------------------ | ---------------- | +| genome | vep_filters | +| mito_name | vep_plugin_files | +| vcfanno_resources | | +| vcfanno_toml | | +| vep_cache_version | | +| vep_cache | | +| score_config_mt | | +| variant_consequences_snv | | ##### 10. Mobile element annotation @@ -279,6 +284,7 @@ no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sampl | mobile_element_svdb_annotations1 | | | vep_cache_version | | | vep_cache | | +| variant_consequences_sv | | 1 A CSV file that describes the databases (VCFs) used by SVDB for annotating mobile elements with allele frequencies. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/svdb_querydb_files.csv). From 66194d68647ea43eaf3ee47483acc30c094ac5ca Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 21:58:57 +0100 Subject: [PATCH 319/387] prettier --- nextflow_schema.json | 57 ++++++++------------------------------------ 1 file changed, 10 insertions(+), 47 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 47c4f0ae..a5b71b42 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,9 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "outdir" - ], + "required": ["outdir"], "properties": { "input": { "type": "string", @@ -425,11 +423,7 @@ "description": "Path to the file containing HGNC_IDs of interest on separate lines." } }, - "required": [ - "fasta", - "intervals_wgs", - "intervals_y" - ] + "required": ["fasta", "intervals_wgs", "intervals_y"] }, "analysis_options": { "title": "Analysis options", @@ -442,11 +436,7 @@ "default": "wgs", "description": "Specifies which analysis type for the pipeline- either 'wgs','wes','mito'. This changes resources consumed and tools used.", "fa_icon": "fas fa-align-center", - "enum": [ - "wgs", - "wes", - "mito" - ] + "enum": ["wgs", "wes", "mito"] }, "gens_switch": { "type": "boolean", @@ -458,20 +448,14 @@ "default": "illumina", "description": "Specifies which analysis type for the pipeline- either 'wgs','wes','mito'. This changes resources consumed and tools used.", "fa_icon": "fas fa-align-center", - "enum": [ - "illumina" - ] + "enum": ["illumina"] }, "ngsbits_samplegender_method": { "type": "string", "default": "xy", "description": "Method selection for ngs-bits samplegender", "fa_icon": "fas fa-align-center", - "enum": [ - "xy", - "hetx", - "sry" - ] + "enum": ["xy", "hetx", "sry"] }, "run_rtgvcfeval": { "type": "boolean", @@ -563,11 +547,7 @@ "default": "bwamem2", "description": "Specifies the alignment algorithm to use - available options are 'bwamem2' and 'sentieon'.", "fa_icon": "fas fa-align-center", - "enum": [ - "bwa", - "bwamem2", - "sentieon" - ] + "enum": ["bwa", "bwamem2", "sentieon"] }, "min_trimmed_length": { "type": "integer", @@ -628,21 +608,14 @@ "default": "deepvariant", "description": "Specifies the variant caller to use - available options are 'deepvariant' and 'sentieon'.", "fa_icon": "fas fa-random", - "enum": [ - "deepvariant", - "sentieon" - ] + "enum": ["deepvariant", "sentieon"] }, "variant_type": { "type": "string", "default": "snp,indel", "description": "Specifies the variant types for sentieon variant caller.", "fa_icon": "fas fa-align-center", - "enum": [ - "snp", - "indel", - "snp,indel" - ] + "enum": ["snp", "indel", "snp,indel"] } } }, @@ -669,10 +642,7 @@ "default": 110, "description": "Specify the version of the VEP cache provided to the `--vep_cache` option.", "fa_icon": "fas fa-align-center", - "enum": [ - 107, - 110 - ] + "enum": [107, 110] } } }, @@ -784,14 +754,7 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { From c7fe536d8ca9e013b9a99a3618354b8deccc4732 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 2 Feb 2024 21:59:25 +0100 Subject: [PATCH 320/387] remove whitespace --- workflows/raredisease.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 3d8984c5..69b7f031 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -288,9 +288,9 @@ workflow RAREDISEASE { ch_target_intervals = ch_references.target_intervals ch_variant_catalog = params.variant_catalog ? Channel.fromPath(params.variant_catalog).map { it -> [[id:it[0].simpleName],it]}.collect() : Channel.value([[],[]]) - ch_variant_consequences_snv = params.variant_consequences_snv ? Channel.fromPath(params.variant_consequences_snv).collect() + ch_variant_consequences_snv = params.variant_consequences_snv ? Channel.fromPath(params.variant_consequences_snv).collect() : Channel.value([]) - ch_variant_consequences_sv = params.variant_consequences_sv ? Channel.fromPath(params.variant_consequences_sv).collect() + ch_variant_consequences_sv = params.variant_consequences_sv ? Channel.fromPath(params.variant_consequences_sv).collect() : Channel.value([]) ch_vcfanno_resources = params.vcfanno_resources ? Channel.fromPath(params.vcfanno_resources).splitText().map{it -> it.trim()}.collect() : Channel.value([]) From 083cf77100ccbf2c8ba8538efe0cc4b00bedf1d5 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 4 Feb 2024 03:04:36 +0100 Subject: [PATCH 321/387] add vep_filters_scout_fmt --- CHANGELOG.md | 1 + conf/modules/annotate_mobile_elements.config | 2 +- conf/modules/generate_clinical_set.config | 6 +- docs/usage.md | 64 +++++++++---------- main.nf | 1 + nextflow_schema.json | 7 ++ .../local/annotate_mobile_elements.nf | 4 +- subworkflows/local/generate_clinical_set.nf | 16 +++-- workflows/raredisease.nf | 41 ++++++++++-- 9 files changed, 91 insertions(+), 51 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index db07d7eb..7e5331a4 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -27,6 +27,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New workflow for annotating mobile elements [#483](https://github.com/nf-core/raredisease/pull/483) - Added a functionality to subsample mitochondrial alignment, and a new parameter `skip_mt_subsample` to skip the subworkflow [#508](https://github.com/nf-core/raredisease/pull/508). - Chromograph to plot coverage across chromosomes [#507](https://github.com/nf-core/raredisease/pull/507) +- Added a new parameter `vep_filters_scout_fmt` to supply a bed like file exported by scout to be used in filter_vep [#511](https://github.com/nf-core/raredisease/pull/511). ### `Changed` diff --git a/conf/modules/annotate_mobile_elements.config b/conf/modules/annotate_mobile_elements.config index dec67a22..aa119729 100644 --- a/conf/modules/annotate_mobile_elements.config +++ b/conf/modules/annotate_mobile_elements.config @@ -61,7 +61,7 @@ process { withName: '.*:ANNOTATE_MOBILE_ELEMENTS:GENERATE_CLINICAL_SET_ME:ENSEMBLVEP_FILTERVEP' { ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_me_${meta.set}" } - ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } + ext.args = { "--filter \"HGNC_ID in ${meta.hgnc_ids.join(',')}\"" } } withName: '.*:ANNOTATE_MOBILE_ELEMENTS:GENERATE_CLINICAL_SET_ME:TABIX_BGZIP' { diff --git a/conf/modules/generate_clinical_set.config b/conf/modules/generate_clinical_set.config index 8de8d594..794e284b 100644 --- a/conf/modules/generate_clinical_set.config +++ b/conf/modules/generate_clinical_set.config @@ -21,7 +21,7 @@ process { withName: '.*:GENERATE_CLINICAL_SET_SNV:ENSEMBLVEP_FILTERVEP' { ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_snv_${meta.set}" } - ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } + ext.args = { "--filter \"HGNC_ID in ${meta.hgnc_ids.join(',')}\"" } } withName: '.*:GENERATE_CLINICAL_SET_SNV:TABIX_BGZIP' { @@ -41,7 +41,7 @@ process { withName: '.*:GENERATE_CLINICAL_SET_SV:ENSEMBLVEP_FILTERVEP' { ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_sv_${meta.set}" } - ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } + ext.args = { "--filter \"HGNC_ID in ${meta.hgnc_ids.join(',')}\"" } } withName: '.*:GENERATE_CLINICAL_SET_SV:TABIX_BGZIP' { @@ -61,7 +61,7 @@ process { withName: '.*:GENERATE_CLINICAL_SET_MT:ENSEMBLVEP_FILTERVEP' { ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_mt_${meta.set}" } - ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } + ext.args = { "--filter \"HGNC_ID in ${meta.hgnc_ids.join(',')}\"" } } withName: '.*:GENERATE_CLINICAL_SET_MT:TABIX_BGZIP' { diff --git a/docs/usage.md b/docs/usage.md index 8f1ec601..1fb9e24a 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -221,15 +221,15 @@ The mandatory and optional parameters for each category are tabulated below. ##### 7. SNV annotation & Ranking -| Mandatory | Optional | -| ----------------------------- | ------------------------------ | -| genome1 | reduced_penetrance7 | -| vcfanno_resources2 | vcfanno_lua | -| vcfanno_toml3 | vep_filters8 | -| vep_cache_version | cadd_resources9 | -| vep_cache4 | vep_plugin_files10 | -| gnomad_af5 | | -| score_config_snv6 | | +| Mandatory | Optional | +| ----------------------------- | --------------------------------------------- | +| genome1 | reduced_penetrance7 | +| vcfanno_resources2 | vcfanno_lua | +| vcfanno_toml3 | vep_filters/vep_filters_scout_fmt8 | +| vep_cache_version | cadd_resources9 | +| vep_cache4 | vep_plugin_files10 | +| gnomad_af5 | | +| score_config_snv6 | | 1Genome version is used by VEP. You have the option to choose between GRCh37 and GRCh38.
2Path to VCF files and their indices used by vcfanno. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/vcfanno_resources.txt).
@@ -241,7 +241,7 @@ See example cache [here](https://raw.githubusercontent.com/nf-core/test-datasets no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/gnomad_reformated.tab.gz).
6Used by GENMOD for ranking the variants. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/rank_model_snv.ini).
7Used by GENMOD while modeling the variants. Contains a list of loci that show [reduced penetrance](https://medlineplus.gov/genetics/understanding/inheritance/penetranceexpressivity/) in people. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/reduced_penetrance.tsv).
-8 This file contains a list of candidate genes (with [HGNC](https://www.genenames.org/) IDs) that is used to split the variants into canditate variants and research variants. Research variants contain all the variants, while candidate variants are a subset of research variants and are associated with candidate genes. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/hgnc.txt). Not required if --skip_vep_filter is set to true.
+8 File containing a list of candidate genes (with [HGNC](https://www.genenames.org/) IDs) that is used to split the variants into canditate variants and research variants. This file can either contain IDs on separate lines (`vep_filter`) or in the format exported by [scout](https://github.com/Clinical-Genomics/scout) (`vep_filters_scout_fmt`). Research variants contain all the variants, while candidate variants are a subset of research variants and are associated with candidate genes. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/hgnc.txt). Not required if --skip_vep_filter is set to true.
9Path to a folder containing cadd annotations. Equivalent of the data/annotations/ folder described [here](https://github.com/kircherlab/CADD-scripts/#manual-installation), and it is used to calculate CADD scores for small indels.
10A CSV file that describes the files used by VEP's named and custom plugins. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/vep_files.csv).
@@ -249,36 +249,36 @@ no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sampl ##### 8. SV annotation & Ranking -| Mandatory | Optional | -| ---------------------------------------------- | ------------------ | -| genome | reduced_penetrance | -| svdb_query_dbs/svdb_query_bedpedbs1 | | -| vep_cache_version | vep_filters | -| vep_cache | vep_plugin_files | -| score_config_sv | | +| Mandatory | Optional | +| ---------------------------------------------- | --------------------------------- | +| genome | reduced_penetrance | +| svdb_query_dbs/svdb_query_bedpedbs1 | | +| vep_cache_version | vep_filters/vep_filters_scout_fmt | +| vep_cache | vep_plugin_files | +| score_config_sv | | 1 A CSV file that describes the databases (VCFs or BEDPEs) used by SVDB for annotating structural variants. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/svdb_querydb_files.csv). Information about the column headers can be found [here](https://github.com/J35P312/SVDB#Query). ##### 9. Mitochondrial annotation -| Mandatory | Optional | -| ----------------- | ---------------- | -| genome | vep_filters | -| mito_name | vep_plugin_files | -| vcfanno_resources | | -| vcfanno_toml | | -| vep_cache_version | | -| vep_cache | | -| score_config_mt | | +| Mandatory | Optional | +| ----------------- | --------------------------------- | +| genome | vep_filters/vep_filters_scout_fmt | +| mito_name | vep_plugin_files | +| vcfanno_resources | | +| vcfanno_toml | | +| vep_cache_version | | +| vep_cache | | +| score_config_mt | | ##### 10. Mobile element annotation -| Mandatory | Optional | -| ------------------------------------------- | ----------- | -| genome | vep_filters | -| mobile_element_svdb_annotations1 | | -| vep_cache_version | | -| vep_cache | | +| Mandatory | Optional | +| ------------------------------------------- | --------------------------------- | +| genome | vep_filters/vep_filters_scout_fmt | +| mobile_element_svdb_annotations1 | | +| vep_cache_version | | +| vep_cache | | 1 A CSV file that describes the databases (VCFs) used by SVDB for annotating mobile elements with allele frequencies. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/svdb_querydb_files.csv). diff --git a/main.nf b/main.nf index 72b342a3..e50414cf 100644 --- a/main.nf +++ b/main.nf @@ -48,6 +48,7 @@ params.svdb_query_dbs = WorkflowMain.getGenomeAttribute(params, params.target_bed = WorkflowMain.getGenomeAttribute(params, 'target_bed') params.variant_catalog = WorkflowMain.getGenomeAttribute(params, 'variant_catalog') params.vep_filters = WorkflowMain.getGenomeAttribute(params, 'vep_filters') +params.vep_filters_scout_fmt = WorkflowMain.getGenomeAttribute(params, 'vep_filters_scout_fmt') params.vcf2cytosure_blacklist = WorkflowMain.getGenomeAttribute(params, 'vcf2cytosure_blacklist') params.vcfanno_resources = WorkflowMain.getGenomeAttribute(params, 'vcfanno_resources') params.vcfanno_toml = WorkflowMain.getGenomeAttribute(params, 'vcfanno_toml') diff --git a/nextflow_schema.json b/nextflow_schema.json index 79045f6d..8501516d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -421,6 +421,13 @@ "format": "path", "fa_icon": "fas fa-file-csv", "description": "Path to the file containing HGNC_IDs of interest on separate lines." + }, + "vep_filters_scout_fmt": { + "type": "string", + "exists": true, + "format": "path", + "fa_icon": "fas fa-table", + "description": "Path to a bed like file exported by scout, which contains HGNC_IDs to be used in filter_vep." } }, "required": ["fasta", "intervals_wgs", "intervals_y"] diff --git a/subworkflows/local/annotate_mobile_elements.nf b/subworkflows/local/annotate_mobile_elements.nf index 362474ea..53fecd0a 100644 --- a/subworkflows/local/annotate_mobile_elements.nf +++ b/subworkflows/local/annotate_mobile_elements.nf @@ -20,7 +20,7 @@ workflow ANNOTATE_MOBILE_ELEMENTS { ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] ch_vep_cache // channel: [mandatory] [ path(cache) ] ch_variant_consequences // channel: [mandatory] [ path(consequences) ] - ch_vep_filters // channel: [mandatory] [ path(vep_filter) ] + ch_hgnc_ids // channel: [mandatory] [ val(hgnc_ids) ] val_vep_genome // string: [mandatory] GRCh37 or GRCh38 val_vep_cache_version // string: [mandatory] default: 107 ch_vep_extra_files // channel: [mandatory] [ path(files) ] @@ -79,7 +79,7 @@ workflow ANNOTATE_MOBILE_ELEMENTS { GENERATE_CLINICAL_SET_ME( BCFTOOLS_VIEW_FILTER.out.vcf, - ch_vep_filters + ch_hgnc_ids ) ANNOTATE_CSQ_PLI_ME( diff --git a/subworkflows/local/generate_clinical_set.nf b/subworkflows/local/generate_clinical_set.nf index e877c39b..87250ff9 100644 --- a/subworkflows/local/generate_clinical_set.nf +++ b/subworkflows/local/generate_clinical_set.nf @@ -8,25 +8,29 @@ include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix' workflow GENERATE_CLINICAL_SET { take: - ch_vcf // channel: [mandatory] [ val(meta), path(vcf) ] - ch_vep_filters // channel: [mandatory] [ path(feature_file) ] + ch_vcf // channel: [mandatory] [ val(meta), path(vcf) ] + ch_hgnc_ids // channel: [mandatory] [ val(hgnc_ids) ] main: ch_versions = Channel.empty() ch_vcf - .multiMap { meta, vcf -> - clinical: [ meta + [ set: "clinical" ], vcf ] + .combine(ch_hgnc_ids) + .multiMap { meta, vcf, ids -> + clinical: [ meta + [ set: "clinical", hgnc_ids:ids ], vcf ] research: [ meta + [ set: "research" ], vcf ] } .set { ch_clin_research_vcf } ENSEMBLVEP_FILTERVEP( ch_clin_research_vcf.clinical, - ch_vep_filters + [] ) + .output + .map {meta, vcf -> [ meta - meta.subMap('hgnc_ids'), vcf ]} + .set { ch_filtervep_out } - TABIX_BGZIP( ENSEMBLVEP_FILTERVEP.out.output ) + TABIX_BGZIP( ch_filtervep_out ) ch_clin_research_vcf.research .mix( TABIX_BGZIP.out.output ) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 650ca446..f6908f79 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -68,7 +68,13 @@ if (!params.skip_germlinecnvcaller) { } if (!params.skip_vep_filter) { - mandatoryParams += ["vep_filters"] + if (!params.vep_filters && !params.vep_filters_scout_fmt) { + println("params.vep_filters or params.vep_filters_scout_fmt should be set.") + missingParamsCount += 1 + } else if (params.vep_filters && params.vep_filters_scout_fmt) { + println("Either params.vep_filters or params.vep_filters_scout_fmt should be set.") + missingParamsCount += 1 + } } if (!params.skip_me_annotation) { @@ -301,8 +307,10 @@ workflow RAREDISEASE { : ( params.vep_cache ? Channel.fromPath(params.vep_cache).collect() : Channel.value([]) ) ch_vep_extra_files_unsplit = params.vep_plugin_files ? Channel.fromPath(params.vep_plugin_files).collect() : Channel.value([]) - ch_vep_filters = params.vep_filters ? Channel.fromPath(params.vep_filters).collect() - : Channel.value([]) + ch_vep_filters_std_fmt = params.vep_filters ? Channel.fromPath(params.vep_filters).splitCsv().collect() + : Channel.empty() + ch_vep_filters_scout_fmt = params.vep_filters_scout_fmt ? Channel.fromPath(params.vep_filters_scout_fmt).collect() + : Channel.empty() ch_versions = ch_versions.mix(ch_references.versions) // SV caller priority @@ -327,6 +335,13 @@ workflow RAREDISEASE { .set {ch_vep_extra_files} } + // Read and store hgnc ids in a channel + ch_vep_filters_scout_fmt + .map { it -> parseHgncIds(it.text) } + .mix (ch_vep_filters_std_fmt) + .toList() + .set {ch_hgnc_ids} + // Input QC if (!params.skip_fastqc) { FASTQC (ch_reads) @@ -484,7 +499,7 @@ workflow RAREDISEASE { GENERATE_CLINICAL_SET_SV( ch_sv_annotate.vcf_ann, - ch_vep_filters + ch_hgnc_ids ) ch_versions = ch_versions.mix(GENERATE_CLINICAL_SET_SV.out.versions) @@ -529,7 +544,7 @@ workflow RAREDISEASE { GENERATE_CLINICAL_SET_SNV( ch_snv_annotate.vcf_ann, - ch_vep_filters + ch_hgnc_ids ) ch_versions = ch_versions.mix(GENERATE_CLINICAL_SET_SNV.out.versions) @@ -571,7 +586,7 @@ workflow RAREDISEASE { GENERATE_CLINICAL_SET_MT( ch_mt_annotate.vcf_ann, - ch_vep_filters + ch_hgnc_ids ) ch_versions = ch_versions.mix(GENERATE_CLINICAL_SET_MT.out.versions) @@ -664,7 +679,7 @@ workflow RAREDISEASE { ch_genome_dictionary, ch_vep_cache, ch_variant_consequences, - ch_vep_filters, + ch_hgnc_ids, params.genome, params.vep_cache_version, ch_vep_extra_files @@ -793,6 +808,18 @@ def create_case_channel(List rows) { return case_info } +// create hgnc list +def parseHgncIds(List text) { + def ids = [] + lines = text[0].tokenize("\n") + for(int i = 0; i Date: Sun, 4 Feb 2024 11:48:55 +0100 Subject: [PATCH 322/387] add subsample skip to configs --- conf/test.config | 3 ++- conf/test_one_sample.config | 1 + 2 files changed, 3 insertions(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index fd2f873e..1afc2549 100644 --- a/conf/test.config +++ b/conf/test.config @@ -30,6 +30,7 @@ params { skip_haplocheck = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_qualimap = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI + skip_mt_subsample = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip subsample on Github CI // Input data input = 'https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/testdata/samplesheet_trio.csv' @@ -56,7 +57,7 @@ params { vcfanno_resources = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_resources.txt" vcfanno_toml = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_config.toml" vep_cache = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_cache_and_plugins.tar.gz" - vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" + vep_filters = "/home/ramprasad.neethiraj/nextflow/raredisease/hgnc_test.txt" vep_cache_version = 107 vep_plugin_files = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_files.csv" } diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index d521a8a3..f73e7520 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -30,6 +30,7 @@ params { skip_haplocheck = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_qualimap = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI + skip_mt_subsample = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip subsample on Github CI // Input data input = 'https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/testdata/samplesheet_single.csv' From 7da3f2db16e498429a547517dfd3597d01699598 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 4 Feb 2024 11:52:43 +0100 Subject: [PATCH 323/387] update file path --- conf/test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index 1afc2549..e542fa50 100644 --- a/conf/test.config +++ b/conf/test.config @@ -57,7 +57,7 @@ params { vcfanno_resources = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_resources.txt" vcfanno_toml = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_config.toml" vep_cache = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_cache_and_plugins.tar.gz" - vep_filters = "/home/ramprasad.neethiraj/nextflow/raredisease/hgnc_test.txt" + vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" vep_cache_version = 107 vep_plugin_files = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_files.csv" } From 41349cf6e6191b9af4aa27c89336e007f1164368 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 4 Feb 2024 20:19:13 +0100 Subject: [PATCH 324/387] Free space step in ci.yaml --- .github/workflows/ci.yml | 7 +++++++ 1 file changed, 7 insertions(+) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index a97b039d..276a9ab0 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -30,6 +30,13 @@ jobs: - "-profile test,docker" - "-profile test_one_sample,docker" steps: + - name: Free some space + run: | + sudo rm -rf /usr/share/dotnet + sudo rm -rf /opt/ghc + sudo rm -rf "/usr/local/share/boost" + sudo rm -rf "$AGENT_TOOLSDIRECTORY" + - name: Check out pipeline code uses: actions/checkout@v4 From 8d120cd1f452ce92b77edcffb4023687b5cdf59a Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 6 Feb 2024 00:05:51 +0100 Subject: [PATCH 325/387] add rhocall_viz --- conf/modules/annotate_rhocallviz.config | 46 ++++++++++++++++ subworkflows/local/annotate_genome_snvs.nf | 7 +++ .../local/annotation/annotate_rhocallviz.nf | 52 +++++++++++++++++++ workflows/raredisease.nf | 4 +- 4 files changed, 108 insertions(+), 1 deletion(-) create mode 100644 conf/modules/annotate_rhocallviz.config create mode 100644 subworkflows/local/annotation/annotate_rhocallviz.nf diff --git a/conf/modules/annotate_rhocallviz.config b/conf/modules/annotate_rhocallviz.config new file mode 100644 index 00000000..d5f09ab9 --- /dev/null +++ b/conf/modules/annotate_rhocallviz.config @@ -0,0 +1,46 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. + ext.when = Conditional clause +---------------------------------------------------------------------------------------- +*/ + +// +// rhocall viz options +// + +process { + + withName: '.*ANNOTATE_GENOME_SNVS:ANNOTATE_RHOCALLVIZ:BCFTOOLS_VIEW' { + ext.prefix = { "${meta.sample}" } + ext.args = { "--output-type z --min-ac 1 --samples ${meta.sample}" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:ANNOTATE_RHOCALLVIZ:BCFTOOLS_ROH' { + ext.prefix = { "${meta.sample}" } + ext.args = { "--AF-tag GNOMADAF --skip-indels" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:ANNOTATE_RHOCALLVIZ:BCFTOOLS_VIEW_UNCOMPRESS' { + ext.prefix = { "${meta.sample}" } + ext.args = { "--output-type v" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:ANNOTATE_RHOCALLVIZ:RHOCALL_VIZ' { + ext.prefix = { "${meta.sample}_rhocallviz" } + ext.args = { "--aftag GNOMADAF --wig" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:ANNOTATE_RHOCALLVIZ:UCSC_WIGTOBIGWIG' { + ext.prefix = { "${meta.sample}_rhocallviz" } + ext.args = { "-clip" } + } + + +} diff --git a/subworkflows/local/annotate_genome_snvs.nf b/subworkflows/local/annotate_genome_snvs.nf index 9e3d74d6..fe59303d 100644 --- a/subworkflows/local/annotate_genome_snvs.nf +++ b/subworkflows/local/annotate_genome_snvs.nf @@ -19,6 +19,7 @@ include { TABIX_TABIX as TABIX_BCFTOOLS_CONCAT } from '../../modules/nf-core/ta include { TABIX_TABIX as TABIX_BCFTOOLS_VIEW } from '../../modules/nf-core/tabix/tabix/main' include { GATK4_SELECTVARIANTS } from '../../modules/nf-core/gatk4/selectvariants/main' include { ANNOTATE_CADD } from './annotation/annotate_cadd' +include { ANNOTATE_RHOCALLVIZ } from './annotation/annotate_rhocallviz' workflow ANNOTATE_GENOME_SNVS { @@ -35,8 +36,10 @@ workflow ANNOTATE_GENOME_SNVS { ch_vep_cache // channel: [mandatory] [ path(cache) ] ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_gnomad_af // channel: [optional] [ path(tab), path(tbi) ] + ch_samples // channel: [mandatory] [ val(sample_meta) ] ch_split_intervals // channel: [mandatory] [ path(intervals) ] ch_vep_extra_files // channel: [mandatory] [ path(files) ] + ch_genome_chrsizes // channel: [mandatory] [ path(sizes) ] main: ch_cadd_vcf = Channel.empty() @@ -77,6 +80,9 @@ workflow ANNOTATE_GENOME_SNVS { ZIP_TABIX_VCFANNO (VCFANNO.out.vcf) + //rhocall_viz + ANNOTATE_RHOCALLVIZ(ZIP_TABIX_VCFANNO.out.gz_tbi, ch_samples, ch_genome_chrsizes) + BCFTOOLS_VIEW(ZIP_TABIX_VCFANNO.out.gz_tbi, [], [], []) // filter on frequencies TABIX_BCFTOOLS_VIEW (BCFTOOLS_VIEW.out.vcf) @@ -171,6 +177,7 @@ workflow ANNOTATE_GENOME_SNVS { ch_versions = ch_versions.mix(TABIX_VEP.out.versions.first()) ch_versions = ch_versions.mix(BCFTOOLS_CONCAT.out.versions) ch_versions = ch_versions.mix(TABIX_BCFTOOLS_CONCAT.out.versions) + ch_versions = ch_versions.mix(ANNOTATE_RHOCALLVIZ.out.versions) emit: vcf_ann = ch_vep_ann // channel: [ val(meta), path(vcf) ] diff --git a/subworkflows/local/annotation/annotate_rhocallviz.nf b/subworkflows/local/annotation/annotate_rhocallviz.nf new file mode 100644 index 00000000..37aeb02d --- /dev/null +++ b/subworkflows/local/annotation/annotate_rhocallviz.nf @@ -0,0 +1,52 @@ +// +// A subworkflow to plot binned zygosity and RHO-regions. +// + +include { BCFTOOLS_VIEW } from '../../../modules/nf-core/bcftools/view/main' +include { TABIX_TABIX } from '../../../modules/nf-core/tabix/tabix/main' +include { BCFTOOLS_ROH } from '../../../modules/nf-core/bcftools/roh/main' +include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_UNCOMPRESS } from '../../../modules/nf-core/bcftools/view/main' +include { RHOCALL_VIZ } from '../../../modules/nf-core/rhocall/viz/main' +include { UCSC_WIGTOBIGWIG } from '../../../modules/nf-core/ucsc/wigtobigwig/main' + +workflow ANNOTATE_RHOCALLVIZ { + + take: + ch_vcf_tbi // channel: [mandatory] [ val(meta), path(vcf), path(tbi) ] + ch_samples // channel: [mandatory] [ val(sample_meta) ] + ch_genome_chrsizes // channel: [mandatory] [ path(sizes) ] + + main: + ch_versions = Channel.empty() + + ch_vcf_tbi + .combine(ch_samples) + .map {meta, vcf, tbi, meta2 -> return [meta2,vcf,tbi]} + .set { ch_rhocall_viz } + + BCFTOOLS_VIEW(ch_rhocall_viz, [],[],[]) + + TABIX_TABIX(BCFTOOLS_VIEW.out.vcf) + + BCFTOOLS_VIEW.out.vcf + .join(TABIX_TABIX.out.tbi) + .set {ch_roh_in } + + BCFTOOLS_ROH(ch_roh_in, [[],[]], [], [], [], []) + + BCFTOOLS_VIEW_UNCOMPRESS(ch_roh_in,[],[],[]) + + RHOCALL_VIZ( BCFTOOLS_VIEW_UNCOMPRESS.out.vcf, BCFTOOLS_ROH.out.roh) + + UCSC_WIGTOBIGWIG(RHOCALL_VIZ.out.wig, ch_genome_chrsizes) + + ch_versions = ch_versions.mix(BCFTOOLS_VIEW.out.versions.first()) + ch_versions = ch_versions.mix(TABIX_TABIX.out.versions.first()) + ch_versions = ch_versions.mix(BCFTOOLS_ROH.out.versions.first()) + ch_versions = ch_versions.mix(BCFTOOLS_VIEW_UNCOMPRESS.out.versions.first()) + ch_versions = ch_versions.mix(RHOCALL_VIZ.out.versions.first()) + ch_versions = ch_versions.mix(UCSC_WIGTOBIGWIG.out.versions.first()) + + emit: + versions = ch_versions // channel: [ path(versions.yml) ] +} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 650ca446..13f8458f 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -522,8 +522,10 @@ workflow RAREDISEASE { ch_vep_cache, ch_genome_fasta, ch_gnomad_af, + ch_samples, ch_scatter_split_intervals, - ch_vep_extra_files + ch_vep_extra_files, + ch_genome_chrsizes ).set { ch_snv_annotate } ch_versions = ch_versions.mix(ch_snv_annotate.versions) From 6e4ee90ecc4ccc4dd2a5956205eae82da8ee14ac Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 6 Feb 2024 00:39:10 +0100 Subject: [PATCH 326/387] output docs --- conf/modules/annotate_rhocallviz.config | 12 ++++++++++-- docs/output.md | 14 ++++++++++++++ 2 files changed, 24 insertions(+), 2 deletions(-) diff --git a/conf/modules/annotate_rhocallviz.config b/conf/modules/annotate_rhocallviz.config index d5f09ab9..cfeb8d7e 100644 --- a/conf/modules/annotate_rhocallviz.config +++ b/conf/modules/annotate_rhocallviz.config @@ -35,12 +35,20 @@ process { withName: '.*ANNOTATE_GENOME_SNVS:ANNOTATE_RHOCALLVIZ:RHOCALL_VIZ' { ext.prefix = { "${meta.sample}_rhocallviz" } ext.args = { "--aftag GNOMADAF --wig" } + publishDir = [ + path: { "${params.outdir}/annotate_snv/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } withName: '.*ANNOTATE_GENOME_SNVS:ANNOTATE_RHOCALLVIZ:UCSC_WIGTOBIGWIG' { ext.prefix = { "${meta.sample}_rhocallviz" } ext.args = { "-clip" } + publishDir = [ + path: { "${params.outdir}/annotate_snv/genome/${meta.sample}_rhocallviz" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } - - } diff --git a/docs/output.md b/docs/output.md index 37f7d4f8..2f782cf4 100644 --- a/docs/output.md +++ b/docs/output.md @@ -52,6 +52,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [VEP](#vep) - [UPD](#upd) - [Chromograph](#chromograph) + - [Rhocall viz](#rhocall-viz) - [Annotation - SV](#annotation---sv) - [SVDB query](#svdb-query) - [VEP](#vep-1) @@ -403,6 +404,19 @@ Based on VEP annotations, custom scripts used by the pipeline further annotate e
+#### Rhocall viz + +[Rhocall viz](https://github.com/dnil/rhocall) plots binned zygosity and RHO-regions. + +
+Output files + +- `annotate_snv/genome/_rhocallviz/_rhocallviz.bed`: file containing regions of homozygosity in bed format. +- `annotate_snv/genome/_rhocallviz/_rhocallviz.wig`: file containing the fraction of homozygous SNPs in wig format. +- `annotate_snv/genome/_rhocallviz/_rhocallviz.bw`: file containing the fraction of homozygous SNPs in bigwig format. + +
+ ### Annotation - SV #### SVDB query From fb2529fab34e965d7d798a908e7f18bec3f69122 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 6 Feb 2024 14:04:12 +0100 Subject: [PATCH 327/387] add bcftools view --- modules/nf-core/bcftools/view/main.nf | 18 ++++++++++++++---- modules/nf-core/bcftools/view/meta.yml | 2 +- .../bcftools/view/tests/main.nf.test.snap | 12 ++++++------ .../bcftools/view/tests/nextflow.config | 4 ++-- 4 files changed, 23 insertions(+), 13 deletions(-) diff --git a/modules/nf-core/bcftools/view/main.nf b/modules/nf-core/bcftools/view/main.nf index a9d93fa6..f3474885 100644 --- a/modules/nf-core/bcftools/view/main.nf +++ b/modules/nf-core/bcftools/view/main.nf @@ -14,8 +14,8 @@ process BCFTOOLS_VIEW { path(samples) output: - tuple val(meta), path("*.gz") , emit: vcf - path "versions.yml" , emit: versions + tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -26,9 +26,14 @@ process BCFTOOLS_VIEW { def regions_file = regions ? "--regions-file ${regions}" : "" def targets_file = targets ? "--targets-file ${targets}" : "" def samples_file = samples ? "--samples-file ${samples}" : "" + def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf" """ bcftools view \\ - --output ${prefix}.vcf.gz \\ + --output ${prefix}.${extension} \\ ${regions_file} \\ ${targets_file} \\ ${samples_file} \\ @@ -44,8 +49,13 @@ process BCFTOOLS_VIEW { stub: def prefix = task.ext.prefix ?: "${meta.id}" + def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf" """ - touch ${prefix}.vcf.gz + touch ${prefix}.${extension} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/bcftools/view/meta.yml b/modules/nf-core/bcftools/view/meta.yml index eaa12b56..6baa34a6 100644 --- a/modules/nf-core/bcftools/view/meta.yml +++ b/modules/nf-core/bcftools/view/meta.yml @@ -53,7 +53,7 @@ output: - vcf: type: file description: VCF normalized output file - pattern: "*.{vcf.gz}" + pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}" - versions: type: file description: File containing software versions diff --git a/modules/nf-core/bcftools/view/tests/main.nf.test.snap b/modules/nf-core/bcftools/view/tests/main.nf.test.snap index 049ac252..b59be932 100644 --- a/modules/nf-core/bcftools/view/tests/main.nf.test.snap +++ b/modules/nf-core/bcftools/view/tests/main.nf.test.snap @@ -7,23 +7,23 @@ "id": "out", "single_end": false }, - "out.vcf.gz:md5,3c47ba1a6aa4ef9b3ad800175814d739" + "out.vcf:md5,1bcbd0eff25d316ba915d06463aab17b" ] ], [ "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" ] ], - "timestamp": "2023-11-29T14:27:10.724842996" + "timestamp": "2024-02-05T17:12:20.799849895" }, "sarscov2 - [vcf, tbi], [], [], [] - stub": { "content": [ - "out.vcf.gz", + "out.vcf", [ "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" ] ], - "timestamp": "2023-11-29T14:27:17.445846794" + "timestamp": "2024-02-05T16:53:34.652746985" }, "sarscov2 - [vcf, tbi], [], [], []": { "content": [ @@ -33,13 +33,13 @@ "id": "out", "single_end": false }, - "out.vcf.gz:md5,a1e45fe6d2b386fc2611766e5d2937ee" + "out.vcf:md5,8e722884ffb75155212a3fc053918766" ] ], [ "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" ] ], - "timestamp": "2023-11-29T14:27:03.328392594" + "timestamp": "2024-02-05T17:12:14.247465409" } } \ No newline at end of file diff --git a/modules/nf-core/bcftools/view/tests/nextflow.config b/modules/nf-core/bcftools/view/tests/nextflow.config index b05aa504..932e3ba6 100644 --- a/modules/nf-core/bcftools/view/tests/nextflow.config +++ b/modules/nf-core/bcftools/view/tests/nextflow.config @@ -1,3 +1,3 @@ process { - ext.args = '--no-version' -} \ No newline at end of file + ext.args = '--no-version --output-type v' +} From 70edfa3834badd2da192cbef98ffecf480e883f6 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 6 Feb 2024 14:11:34 +0100 Subject: [PATCH 328/387] add rhocall_viz --- modules.json | 7 ++- modules/nf-core/rhocall/viz/environment.yml | 7 +++ modules/nf-core/rhocall/viz/main.nf | 53 ++++++++++++++++++ modules/nf-core/rhocall/viz/meta.yml | 52 ++++++++++++++++++ .../nf-core/rhocall/viz/tests/main.nf.test | 54 +++++++++++++++++++ .../rhocall/viz/tests/main.nf.test.snap | 47 ++++++++++++++++ .../nf-core/rhocall/viz/tests/nextflow.config | 3 ++ modules/nf-core/rhocall/viz/tests/tags.yml | 2 + 8 files changed, 224 insertions(+), 1 deletion(-) create mode 100644 modules/nf-core/rhocall/viz/environment.yml create mode 100644 modules/nf-core/rhocall/viz/main.nf create mode 100644 modules/nf-core/rhocall/viz/meta.yml create mode 100644 modules/nf-core/rhocall/viz/tests/main.nf.test create mode 100644 modules/nf-core/rhocall/viz/tests/main.nf.test.snap create mode 100644 modules/nf-core/rhocall/viz/tests/nextflow.config create mode 100644 modules/nf-core/rhocall/viz/tests/tags.yml diff --git a/modules.json b/modules.json index ffdf8a5f..3f475b74 100644 --- a/modules.json +++ b/modules.json @@ -47,7 +47,7 @@ }, "bcftools/view": { "branch": "master", - "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", + "git_sha": "7157328de1724e26d8ed3b13bb715fde62912896", "installed_by": ["modules"] }, "bedtools/genomecov": { @@ -358,6 +358,11 @@ "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, + "rhocall/viz": { + "branch": "master", + "git_sha": "6ea51097105e91adc656aa965de2f2a7c9c9b923", + "installed_by": ["modules"] + }, "rtgtools/format": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", diff --git a/modules/nf-core/rhocall/viz/environment.yml b/modules/nf-core/rhocall/viz/environment.yml new file mode 100644 index 00000000..6e5b352f --- /dev/null +++ b/modules/nf-core/rhocall/viz/environment.yml @@ -0,0 +1,7 @@ +name: "rhocall_viz" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::rhocall=0.5.1" diff --git a/modules/nf-core/rhocall/viz/main.nf b/modules/nf-core/rhocall/viz/main.nf new file mode 100644 index 00000000..7c4b9d07 --- /dev/null +++ b/modules/nf-core/rhocall/viz/main.nf @@ -0,0 +1,53 @@ +process RHOCALL_VIZ { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/rhocall:0.5.1--py39hbf8eff0_0': + 'biocontainers/rhocall:0.5.1--py39hbf8eff0_0' }" + + input: + tuple val(meta), path(vcf) + tuple val(meta2), path(roh) + + output: + tuple val(meta), path("${prefix}/${prefix}.bed"), emit: bed + tuple val(meta), path("${prefix}/${prefix}.wig"), emit: wig + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + """ + rhocall \\ + viz \\ + $args \\ + -r $roh \\ + --out_dir ${prefix} \\ + $vcf + + mv ${prefix}/output.bed ${prefix}/${prefix}.bed + mv ${prefix}/output.wig ${prefix}/${prefix}.wig + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + rhocall: \$(echo \$(rhocall --version 2>&1) | sed 's/rhocall, version //' ) + END_VERSIONS + """ + + stub: + prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}/${prefix}.bed + touch ${prefix}/${prefix}.wig + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + rhocall: \$(echo \$(rhocall --version 2>&1) | sed 's/rhocall, version //' ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/rhocall/viz/meta.yml b/modules/nf-core/rhocall/viz/meta.yml new file mode 100644 index 00000000..bbcad9ba --- /dev/null +++ b/modules/nf-core/rhocall/viz/meta.yml @@ -0,0 +1,52 @@ +name: "rhocall_viz" +description: Call regions of homozygosity and make tentative UPD calls +keywords: + - roh + - bcftools + - runs_of_homozygosity +tools: + - "rhocall": + description: "Call regions of homozygosity and make tentative UPD calls." + homepage: "https://github.com/dnil/rhocall" + documentation: "https://github.com/dnil/rhocall" + tool_dev_url: "https://github.com/dnil" + licence: ["GPL v3"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - vcf: + type: file + description: VCF file + pattern: "*.{vcf}" + - roh: + type: file + description: Input RHO file produced from rhocall + pattern: "*.{roh}" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - bed: + type: file + description: Bed file containing roh calls + pattern: "*.{bed}" + - wig: + type: file + description: Wig file containing roh calls + pattern: "*.{wig}" + +authors: + - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/rhocall/viz/tests/main.nf.test b/modules/nf-core/rhocall/viz/tests/main.nf.test new file mode 100644 index 00000000..094e7d07 --- /dev/null +++ b/modules/nf-core/rhocall/viz/tests/main.nf.test @@ -0,0 +1,54 @@ +nextflow_process { + + name "Test Process RHOCALL_VIZ" + script "../main.nf" + process "RHOCALL_VIZ" + + tag "modules" + tag "modules_nfcore" + tag "rhocall" + tag "rhocall/viz" + tag "bcftools/roh" + + config "./nextflow.config" + + test("sarscov2 - vcf, roh") { + + + setup { + run("BCFTOOLS_ROH") { + script "../../../bcftools/roh/main.nf" + process { + """ + input[0] = [ [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true)] + + input[1] = [[],[]] + input[2] = [] + input[3] = [] + input[4] = [] + input[5] = [] + """ + } + } + } + when { + process { + """ + input[0] = [ [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true)] + input[1] = BCFTOOLS_ROH.out.roh + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } +} diff --git a/modules/nf-core/rhocall/viz/tests/main.nf.test.snap b/modules/nf-core/rhocall/viz/tests/main.nf.test.snap new file mode 100644 index 00000000..cba4067f --- /dev/null +++ b/modules/nf-core/rhocall/viz/tests/main.nf.test.snap @@ -0,0 +1,47 @@ +{ + "sarscov2 - vcf, roh": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.bed:md5,4579710bbd8e1e4449274d261c439891" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.wig:md5,ab2d23269213f6331f18b7ad6ca94a5f" + ] + ], + "2": [ + "versions.yml:md5,079291120b14dd6b9368dd1cff72518e" + ], + "bed": [ + [ + { + "id": "test" + }, + "test.bed:md5,4579710bbd8e1e4449274d261c439891" + ] + ], + "versions": [ + "versions.yml:md5,079291120b14dd6b9368dd1cff72518e" + ], + "wig": [ + [ + { + "id": "test" + }, + "test.wig:md5,ab2d23269213f6331f18b7ad6ca94a5f" + ] + ] + } + ], + "timestamp": "2024-02-05T17:57:24.70125206" + } +} \ No newline at end of file diff --git a/modules/nf-core/rhocall/viz/tests/nextflow.config b/modules/nf-core/rhocall/viz/tests/nextflow.config new file mode 100644 index 00000000..2217be8e --- /dev/null +++ b/modules/nf-core/rhocall/viz/tests/nextflow.config @@ -0,0 +1,3 @@ +env { + MPLCONFIGDIR = "/tmp" +} diff --git a/modules/nf-core/rhocall/viz/tests/tags.yml b/modules/nf-core/rhocall/viz/tests/tags.yml new file mode 100644 index 00000000..bc2d74a7 --- /dev/null +++ b/modules/nf-core/rhocall/viz/tests/tags.yml @@ -0,0 +1,2 @@ +rhocall/viz: + - "modules/nf-core/rhocall/viz/**" From 41221fdcb4e6dc05a824fc3092db4bba1fe486e6 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 7 Feb 2024 09:37:34 +0100 Subject: [PATCH 329/387] Update CHANGELOG.md Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com> --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 454380f9..7c0256e2 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -27,7 +27,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - New workflow for annotating mobile elements [#483](https://github.com/nf-core/raredisease/pull/483) - Added a functionality to subsample mitochondrial alignment, and a new parameter `skip_mt_subsample` to skip the subworkflow [#508](https://github.com/nf-core/raredisease/pull/508). - Chromograph to plot coverage across chromosomes [#507](https://github.com/nf-core/raredisease/pull/507) -- Added a new parameter `vep_filters_scout_fmt` to supply a bed like file exported by scout to be used in filter_vep [#511](https://github.com/nf-core/raredisease/pull/511). +- Added a new parameter `vep_filters_scout_fmt` to supply a bed-like file exported by scout to be used in filter_vep [#511](https://github.com/nf-core/raredisease/pull/511). - Added two new parameters `variant_consequences_snv` and `variant_consequences_sv` to supply variant consequence files for annotating SNVs and SVs. [#509](https://github.com/nf-core/raredisease/pull/509) ### `Changed` From f6d4d6b51277ed018104c5e5036c7d84b0903498 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 7 Feb 2024 09:37:40 +0100 Subject: [PATCH 330/387] Update nextflow_schema.json Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com> --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 561d1296..9b72083d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -427,7 +427,7 @@ "exists": true, "format": "path", "fa_icon": "fas fa-table", - "description": "Path to a bed like file exported by scout, which contains HGNC_IDs to be used in filter_vep." + "description": "Path to a bed-like file exported by scout, which contains HGNC_IDs to be used in filter_vep." } }, "required": ["fasta", "intervals_wgs", "intervals_y"] From dd2dd7e49125e6b7b5b333c0e102a52713859bba Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 7 Feb 2024 13:59:06 +0100 Subject: [PATCH 331/387] fix errors --- conf/modules/annotate_mobile_elements.config | 2 +- modules.json | 4 ++-- modules/nf-core/bcftools/view/main.nf | 1 + modules/nf-core/rhocall/viz/main.nf | 1 + 4 files changed, 5 insertions(+), 3 deletions(-) diff --git a/conf/modules/annotate_mobile_elements.config b/conf/modules/annotate_mobile_elements.config index aa119729..bed2c0a7 100644 --- a/conf/modules/annotate_mobile_elements.config +++ b/conf/modules/annotate_mobile_elements.config @@ -54,7 +54,7 @@ process { withName: '.*ANNOTATE_MOBILE_ELEMENTS:BCFTOOLS_VIEW_FILTER' { // extend filter with arguments such as --exclude 'INFO/swegen_sva_FRQ > 0.1' - ext.args = { "--apply-filters PASS" } + ext.args = { "--apply-filters PASS --output-type z" } ext.prefix = { "${meta.id}_filter" } } diff --git a/modules.json b/modules.json index 3f475b74..1076a6d6 100644 --- a/modules.json +++ b/modules.json @@ -47,7 +47,7 @@ }, "bcftools/view": { "branch": "master", - "git_sha": "7157328de1724e26d8ed3b13bb715fde62912896", + "git_sha": "1013101da4252623fd7acf19cc581bae91d4f839", "installed_by": ["modules"] }, "bedtools/genomecov": { @@ -360,7 +360,7 @@ }, "rhocall/viz": { "branch": "master", - "git_sha": "6ea51097105e91adc656aa965de2f2a7c9c9b923", + "git_sha": "1013101da4252623fd7acf19cc581bae91d4f839", "installed_by": ["modules"] }, "rtgtools/format": { diff --git a/modules/nf-core/bcftools/view/main.nf b/modules/nf-core/bcftools/view/main.nf index f3474885..5237adc8 100644 --- a/modules/nf-core/bcftools/view/main.nf +++ b/modules/nf-core/bcftools/view/main.nf @@ -48,6 +48,7 @@ process BCFTOOLS_VIEW { """ stub: + def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : diff --git a/modules/nf-core/rhocall/viz/main.nf b/modules/nf-core/rhocall/viz/main.nf index 7c4b9d07..4191ef3b 100644 --- a/modules/nf-core/rhocall/viz/main.nf +++ b/modules/nf-core/rhocall/viz/main.nf @@ -42,6 +42,7 @@ process RHOCALL_VIZ { stub: prefix = task.ext.prefix ?: "${meta.id}" """ + mkdir ${prefix} touch ${prefix}/${prefix}.bed touch ${prefix}/${prefix}.wig From 8178f32a1cfb4776ac1b574a4ea8d37dcf552b58 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 7 Feb 2024 15:23:39 +0100 Subject: [PATCH 332/387] update config --- nextflow.config | 1 + 1 file changed, 1 insertion(+) diff --git a/nextflow.config b/nextflow.config index 69dd2cae..72bb3f34 100644 --- a/nextflow.config +++ b/nextflow.config @@ -337,6 +337,7 @@ includeConfig 'conf/modules/annotate_mobile_elements.config' includeConfig 'conf/modules/generate_clinical_set.config' includeConfig 'conf/modules/variant_evaluation.config' includeConfig 'conf/modules/subsample_mt.config' +includeConfig 'conf/modules/annotate_rhocallviz.config' // Function to ensure that resource requirements don't go beyond // a maximum limit From f10869bbb1e43e2d0ce5d1c98b475e8109166337 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 11 Feb 2024 13:36:41 +0100 Subject: [PATCH 333/387] alignment --- conf/modules/align_MT.config | 25 ++++++++++++------------- conf/modules/align_bwa_bwamem2.config | 8 ++++---- 2 files changed, 16 insertions(+), 17 deletions(-) diff --git a/conf/modules/align_MT.config b/conf/modules/align_MT.config index e88f7e88..5ffec1c0 100644 --- a/conf/modules/align_MT.config +++ b/conf/modules/align_MT.config @@ -21,7 +21,7 @@ process { withName: '.*ALIGN_MT:BWAMEM2_MEM_MT' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.when = { !(params.analysis_type.equals("wes")) } - ext.prefix = { "${meta.id}.sorted" } + ext.prefix = { "${meta.id}_sorted" } } } @@ -29,7 +29,7 @@ process { withName: '.*ALIGN_MT:BWA_MEM_MT' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.when = { !(params.analysis_type.equals("wes")) } - ext.prefix = { "${meta.id}.sorted" } + ext.prefix = { "${meta.id}_sorted" } } } @@ -37,13 +37,13 @@ process { withName: '.*ALIGN_MT:SENTIEON_BWAMEM_MT' { ext.args = { "-M -K 10000000 -R ${meta.read_group}" } ext.when = { !(params.analysis_type.equals("wes"))} - ext.prefix = { "${meta.id}.sorted" } + ext.prefix = { "${meta.id}_sorted" } } } withName: '.*ALIGN_MT:GATK4_MERGEBAMALIGNMENT_MT' { ext.args = '--TMP_DIR . --CREATE_INDEX true --MAX_GAPS -1 --SORT_ORDER queryname --INCLUDE_SECONDARY_ALIGNMENTS false --PAIRED_RUN false --VALIDATION_STRINGENCY LENIENT' - ext.prefix = { "${meta.id}_merged" } + ext.prefix = { "${meta.id}_sorted_merged" } } withName: '.*ALIGN_MT:PICARD_ADDORREPLACEREADGROUPS_MT' { @@ -59,11 +59,11 @@ process { withName: '.*ALIGN_MT:PICARD_MARKDUPLICATES_MT' { ext.args = '--TMP_DIR . --VALIDATION_STRINGENCY LENIENT --CREATE_INDEX true' - ext.prefix = { "${meta.id}_markduplicates" } + ext.prefix = { "${meta.id}_sorted_merged_md" } } withName: '.*ALIGN_MT:SAMTOOLS_SORT_MT' { - ext.prefix = { "${meta.id}_sorted" } + ext.prefix = { "${meta.id}_sorted_merged_md_sorted" } } } @@ -78,7 +78,7 @@ process { withName: '.*ALIGN_MT_SHIFT:BWAMEM2_MEM_MT' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.when = { !(params.analysis_type.equals("wes")) } - ext.prefix = { "${meta.id}.sorted" } + ext.prefix = { "${meta.id}_sorted_shifted" } } } @@ -86,22 +86,21 @@ process { withName: '.*ALIGN_MT_SHIFT:SENTIEON_BWAMEM_MT' { ext.args = { "-M -K 10000000 -R ${meta.read_group}" } ext.when = { !(params.analysis_type.equals("wes"))} - ext.prefix = { "${meta.id}.sorted" } + ext.prefix = { "${meta.id}_sorted_shifted" } } } - if (params.aligner.equals("bwa")) { withName: '.*ALIGN_MT_SHIFT:BWA_MEM_MT' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.when = { !(params.analysis_type.equals("wes")) } - ext.prefix = { "${meta.id}.sorted" } + ext.prefix = { "${meta.id}_sorted_shifted" } } } withName: '.*ALIGN_MT_SHIFT:GATK4_MERGEBAMALIGNMENT_MT' { ext.args = '--TMP_DIR . --CREATE_INDEX true --MAX_GAPS -1 --SORT_ORDER queryname --INCLUDE_SECONDARY_ALIGNMENTS false --PAIRED_RUN false --VALIDATION_STRINGENCY LENIENT' - ext.prefix = { "${meta.id}_merged_shifted" } + ext.prefix = { "${meta.id}_sorted_merged_shifted" } } withName: '.*ALIGN_MT_SHIFT:PICARD_ADDORREPLACEREADGROUPS_MT' { @@ -117,11 +116,11 @@ process { withName: '.*ALIGN_MT_SHIFT:PICARD_MARKDUPLICATES_MT' { ext.args = '--TMP_DIR . --VALIDATION_STRINGENCY LENIENT --CREATE_INDEX true' - ext.prefix = { "${meta.id}_markduplicates_shifted" } + ext.prefix = { "${meta.id}_sorted_merged_md_shifted" } } withName: '.*ALIGN_MT_SHIFT:SAMTOOLS_SORT_MT' { - ext.prefix = { "${meta.id}_sorted_shifted" } + ext.prefix = { "${meta.id}_sorted_merged_md_sorted_shifted" } } } diff --git a/conf/modules/align_bwa_bwamem2.config b/conf/modules/align_bwa_bwamem2.config index 15453e0e..e91360fc 100644 --- a/conf/modules/align_bwa_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2.config @@ -21,14 +21,14 @@ process { withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWAMEM2_MEM' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - ext.prefix = { "${meta.id}.sorted" } - ext.when = { params.aligner.equals("bwamem2")} + ext.prefix = { "${meta.id}_sorted" } + ext.when = { params.aligner.equals("bwamem2") } } withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - ext.prefix = { "${meta.id}.sorted" } - ext.when = { params.aligner.equals("bwa")} + ext.prefix = { "${meta.id}_sorted" } + ext.when = { params.aligner.equals("bwa") } } withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_STATS' { From 04af72affb1223da4f6747ced5897f67cea3932c Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 12 Feb 2024 09:33:17 +0100 Subject: [PATCH 334/387] call sv --- conf/modules/call_sv_germlinecnvcaller.config | 2 +- docs/output.md | 4 ++-- modules.json | 2 +- modules/nf-core/eklipse/main.nf | 8 ++++---- modules/nf-core/eklipse/tests/main.nf.test.snap | 8 ++++---- 5 files changed, 12 insertions(+), 12 deletions(-) diff --git a/conf/modules/call_sv_germlinecnvcaller.config b/conf/modules/call_sv_germlinecnvcaller.config index 6685dc6c..5f85c0f9 100644 --- a/conf/modules/call_sv_germlinecnvcaller.config +++ b/conf/modules/call_sv_germlinecnvcaller.config @@ -39,7 +39,7 @@ process { } withName: '.*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:BCFTOOLS_VIEW' { - ext.prefix = { "${meta.id}_gatkcnv_segments_reffiltered" } + ext.prefix = { "${meta.id}_gatkcnv_segments_refiltered" } ext.args = { '--output-type z --exclude "N_ALT = 0" ' } } } diff --git a/docs/output.md b/docs/output.md index 2f782cf4..4edd4376 100644 --- a/docs/output.md +++ b/docs/output.md @@ -462,8 +462,8 @@ The pipeline for mitochondrial variant discovery, using Mutect2, uses a high sen [eKLIPse](https://github.com/dooguypapua/eKLIPse) allows the detection and quantification of large mtDNA rearrangements. - `call_sv/mitochondria` - - `eKLIPse_deletions.csv`: file containing all predicted deletions. - - `eKLIPse_genes.csv`: file summarizing cumulated deletions per mtDNA gene. + - `eKLIPse__deletions.csv`: file containing all predicted deletions. + - `eKLIPse__genes.csv`: file summarizing cumulated deletions per mtDNA gene. - `eKLIPse_.png`: circos plot. #### Annotation: diff --git a/modules.json b/modules.json index 1076a6d6..e98107a0 100644 --- a/modules.json +++ b/modules.json @@ -113,7 +113,7 @@ }, "eklipse": { "branch": "master", - "git_sha": "0031772de29283811deb989a5b42d0310d35ec22", + "git_sha": "7f265c2db4b1394c9303a3eb51e25d8447767347", "installed_by": ["modules"] }, "ensemblvep/filtervep": { diff --git a/modules/nf-core/eklipse/main.nf b/modules/nf-core/eklipse/main.nf index b50df3c1..98c57789 100644 --- a/modules/nf-core/eklipse/main.nf +++ b/modules/nf-core/eklipse/main.nf @@ -33,8 +33,8 @@ process EKLIPSE { -in infile.txt \\ $args \\ -ref $ref_gb - mv eKLIPse_*/eKLIPse_deletions.csv eKLIPse_deletions.csv - mv eKLIPse_*/eKLIPse_genes.csv eKLIPse_genes.csv + mv eKLIPse_*/eKLIPse_deletions.csv eKLIPse_${prefix}_deletions.csv + mv eKLIPse_*/eKLIPse_genes.csv eKLIPse_${prefix}_genes.csv mv eKLIPse_*/eKLIPse_${prefix}.png eKLIPse_${prefix}.png cat <<-END_VERSIONS > versions.yml @@ -47,8 +47,8 @@ process EKLIPSE { def prefix = task.ext.prefix ?: "${meta.id}" def VERSION = "1.8" """ - touch eKLIPse_deletions.csv - touch eKLIPse_genes.csv + touch eKLIPse_${prefix}_deletions.csv + touch eKLIPse_${prefix}_genes.csv touch eKLIPse_${prefix}.png cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/eklipse/tests/main.nf.test.snap b/modules/nf-core/eklipse/tests/main.nf.test.snap index 87d79dac..5969a1da 100644 --- a/modules/nf-core/eklipse/tests/main.nf.test.snap +++ b/modules/nf-core/eklipse/tests/main.nf.test.snap @@ -1,11 +1,11 @@ { - "eklipse": { + "homo_sapiens [bam]": { "content": [ - "eKLIPse_deletions.csv:md5,8bcfdb8f3e8a63dcd211c2a53b6ca0a7", - "eKLIPse_genes.csv:md5,ec1a1f31d018ca66a46d8a2d17bc8d18", + "eKLIPse_test_deletions.csv:md5,8bcfdb8f3e8a63dcd211c2a53b6ca0a7", + "eKLIPse_test_genes.csv:md5,ec1a1f31d018ca66a46d8a2d17bc8d18", "eKLIPse_test.png:md5,d1ea2a548bc4f8d321b1128a61ea8b0a", "versions.yml:md5,8e6ebaa9abf72ced91c1f56acd10f131" ], - "timestamp": "2023-11-22T18:02:08.245264064" + "timestamp": "2024-02-11T14:55:25.205208435" } } \ No newline at end of file From 55edd59e399054849ca2de4e17472080aff486a8 Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 13 Feb 2024 11:45:58 +0100 Subject: [PATCH 335/387] updating gens subworkflow --- conf/modules/gens.config | 19 +++++-- modules/local/gens/main.nf | 14 ++--- nextflow.config | 4 +- nextflow_schema.json | 10 ++-- subworkflows/local/call_snv.nf | 26 +++++++--- subworkflows/local/gens.nf | 28 +++++++--- .../variant_calling/call_snv_deepvariant.nf | 8 +-- .../variant_calling/call_snv_sentieon.nf | 11 ++-- workflows/raredisease.nf | 52 +++++++++++-------- 9 files changed, 114 insertions(+), 58 deletions(-) diff --git a/conf/modules/gens.config b/conf/modules/gens.config index 3a3f6ccf..fdd798f5 100644 --- a/conf/modules/gens.config +++ b/conf/modules/gens.config @@ -16,17 +16,26 @@ // process { - if (params.gens_switch) { + if (!params.skip_gens && params.analysis_type != "wes") { withName: '.*GENS:.*' { publishDir = [ - path: { "${params.outdir}/gens" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + enabled: false ] } withName: '.*GENS:COLLECTREADCOUNTS' { - ext.args = '--interval-merging-rule OVERLAPPING_ONLY' + ext.args = { [ + '--interval-merging-rule OVERLAPPING_ONLY', + '--format HDF5' + ].join(' ') } + } + + withName: '.*GENS:GENERATE_DATA' { + publishDir = [ + path: { "${params.outdir}/gens" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } } } diff --git a/modules/local/gens/main.nf b/modules/local/gens/main.nf index 34399885..6708e79c 100644 --- a/modules/local/gens/main.nf +++ b/modules/local/gens/main.nf @@ -2,24 +2,26 @@ process GENS { tag "$meta.id" label 'process_medium' - container 'docker.io/raysloks/gens_preproc:1.0.1' + container 'docker.io/clinicalgenomics/gens_preproc:1.0.11' input: tuple val(meta), path(read_counts) - path vcf + tuple val(meta2), path(gvcf) path gnomad_positions output: - tuple val(meta), path('*.cov.bed.gz'), emit: cov - tuple val(meta), path('*.baf.bed.gz'), emit: baf - path "versions.yml" , emit: versions + tuple val(meta), path('*.cov.bed.gz') , emit: cov + tuple val(meta), path('*.cov.bed.gz.tbi'), emit: cov_index + tuple val(meta), path('*.baf.bed.gz') , emit: baf + tuple val(meta), path('*.baf.bed.gz.tbi'), emit: baf_index + path "versions.yml" , emit: versions script: def prefix = task.ext.prefix ?: "${meta.id}" """ generate_gens_data.pl \\ $read_counts \\ - $vcf \\ + $gvcf \\ $prefix \\ $gnomad_positions diff --git a/nextflow.config b/nextflow.config index 72bb3f34..6e55e55a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -28,8 +28,11 @@ params { skip_eklipse = false skip_fastp = false skip_fastqc = false + skip_gens = true skip_germlinecnvcaller = false skip_haplocheck = false + skip_me_annotation = false + skip_mt_annotation = false skip_qualimap = false skip_snv_annotation = false skip_sv_annotation = false @@ -38,7 +41,6 @@ params { skip_mt_subsample = false skip_vcf2cytosure = true skip_vep_filter = false - gens_switch = false cadd_resources = null platform = 'illumina' diff --git a/nextflow_schema.json b/nextflow_schema.json index 9b72083d..1ea97d44 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -445,11 +445,6 @@ "fa_icon": "fas fa-align-center", "enum": ["wgs", "wes", "mito"] }, - "gens_switch": { - "type": "boolean", - "description": "Specifies whether or not to run gens preprocessing subworkflow.", - "fa_icon": "fas fa-toggle-on" - }, "platform": { "type": "string", "default": "illumina", @@ -489,6 +484,11 @@ "description": "Specifies whether or not to skip haplocheck.", "fa_icon": "fas fa-toggle-on" }, + "skip_gens": { + "type": "boolean", + "description": "Specifies whether or not to skip gens preprocessing subworkflow.", + "fa_icon": "fas fa-toggle-on" + }, "skip_germlinecnvcaller": { "type": "boolean", "description": "Specifies whether or not to skip CNV calling using GATK's GermlineCNVCaller", diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index 8d926f0e..3e76ab91 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -33,11 +33,15 @@ workflow CALL_SNV { ch_pcr_indel_model // channel: [optional] [ val(sentieon_dnascope_pcr_indel_model) ] main: - ch_versions = Channel.empty() - ch_deepvar_vcf = Channel.empty() - ch_deepvar_tbi = Channel.empty() - ch_sentieon_vcf = Channel.empty() - ch_sentieon_tbi = Channel.empty() + ch_versions = Channel.empty() + ch_deepvar_vcf = Channel.empty() + ch_deepvar_tbi = Channel.empty() + ch_deepvar_gvcf = Channel.empty() + ch_deepvar_gtbi = Channel.empty() + ch_sentieon_vcf = Channel.empty() + ch_sentieon_tbi = Channel.empty() + ch_sentieon_gvcf = Channel.empty() + ch_sentieon_gtbi = Channel.empty() if (params.variant_caller.equals("deepvariant")) { CALL_SNV_DEEPVARIANT ( // triggered only when params.variant_caller is set as deepvariant @@ -50,6 +54,8 @@ workflow CALL_SNV { ) ch_deepvar_vcf = CALL_SNV_DEEPVARIANT.out.vcf ch_deepvar_tbi = CALL_SNV_DEEPVARIANT.out.tabix + ch_deepvar_gvcf = CALL_SNV_DEEPVARIANT.out.gvcf + ch_deepvar_gtbi = CALL_SNV_DEEPVARIANT.out.gvcf_tabix ch_versions = ch_versions.mix(CALL_SNV_DEEPVARIANT.out.versions) } else if (params.variant_caller.equals("sentieon")) { CALL_SNV_SENTIEON( // triggered only when params.variant_caller is set as sentieon @@ -67,11 +73,15 @@ workflow CALL_SNV { ) ch_sentieon_vcf = CALL_SNV_SENTIEON.out.vcf ch_sentieon_tbi = CALL_SNV_SENTIEON.out.tabix + ch_sentieon_gvcf = CALL_SNV_SENTIEON.out.gvcf + ch_sentieon_gtbi = CALL_SNV_SENTIEON.out.gtbi ch_versions = ch_versions.mix(CALL_SNV_SENTIEON.out.versions) } - ch_vcf = Channel.empty().mix(ch_deepvar_vcf, ch_sentieon_vcf) - ch_tabix = Channel.empty().mix(ch_deepvar_tbi, ch_sentieon_tbi) + ch_vcf = Channel.empty().mix(ch_deepvar_vcf, ch_sentieon_vcf) + ch_tabix = Channel.empty().mix(ch_deepvar_tbi, ch_sentieon_tbi) + ch_gvcf = Channel.empty().mix(ch_deepvar_gvcf, ch_sentieon_gvcf) + ch_gtabix = Channel.empty().mix(ch_deepvar_gtbi, ch_sentieon_gtbi) ch_vcf .join(ch_tabix, failOnMismatch:true, failOnDuplicate:true) @@ -120,6 +130,8 @@ workflow CALL_SNV { genome_vcf = ch_genome_vcf // channel: [ val(meta), path(vcf) ] genome_tabix = ch_genome_tabix // channel: [ val(meta), path(tbi) ] genome_vcf_tabix = ch_genome_vcf_tabix // channel: [ val(meta), path(vcf), path(tbi) ] + genome_gvcf = ch_gvcf // channel: [ val(meta), path(gvcf) ] + genome_gtabix = ch_gtabix // channel: [ val(meta), path(gtbi) ] mt_vcf = POSTPROCESS_MT_CALLS.out.vcf // channel: [ val(meta), path(vcf) ] mt_tabix = POSTPROCESS_MT_CALLS.out.tbi // channel: [ val(meta), path(tbi) ] versions = ch_versions // channel: [ path(versions.yml) ] diff --git a/subworkflows/local/gens.nf b/subworkflows/local/gens.nf index fdee366d..30f94cc3 100644 --- a/subworkflows/local/gens.nf +++ b/subworkflows/local/gens.nf @@ -9,7 +9,7 @@ include { GENS as GENS_GENERATE } from '../../modules/loc workflow GENS { take: ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] - ch_vcf // channel: [mandatory] [ val(meta), path(vcf) ] + ch_gvcf // channel: [mandatory] [ val(meta), path(gvcf) ] ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] ch_interval_list // channel: [mandatory] [ path(interval_list) ] @@ -21,11 +21,27 @@ workflow GENS { main: ch_versions = Channel.empty() - COLLECTREADCOUNTS (ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_sequence_dictionary, ch_interval_list) - - DENOISEREADCOUNTS (COLLECTREADCOUNTS.out.read_counts, ch_pon) - - GENS_GENERATE (DENOISEREADCOUNTS.out.standardized_read_counts, ch_vcf.map { meta, vcf -> vcf }, ch_gnomad_pos) + ch_bam_bai + .combine(ch_interval_list) + .set { ch_bam_bai_intervals } + + COLLECTREADCOUNTS ( + ch_bam_bai_intervals, + ch_genome_fasta, + ch_genome_fai, + ch_genome_dictionary + ) + + DENOISEREADCOUNTS ( + COLLECTREADCOUNTS.out.hdf5, + ch_pon + ) + + GENS_GENERATE ( + DENOISEREADCOUNTS.out.standardized, + ch_gvcf, + ch_gnomad_pos + ) ch_versions = ch_versions.mix(COLLECTREADCOUNTS.out.versions.first()) ch_versions = ch_versions.mix(DENOISEREADCOUNTS.out.versions.first()) diff --git a/subworkflows/local/variant_calling/call_snv_deepvariant.nf b/subworkflows/local/variant_calling/call_snv_deepvariant.nf index 4fc6ab4d..c3477d67 100644 --- a/subworkflows/local/variant_calling/call_snv_deepvariant.nf +++ b/subworkflows/local/variant_calling/call_snv_deepvariant.nf @@ -82,7 +82,9 @@ workflow CALL_SNV_DEEPVARIANT { ch_versions = ch_versions.mix(TABIX_ANNOTATE.out.versions) emit: - vcf = BCFTOOLS_ANNOTATE.out.vcf // channel: [ val(meta), path(vcf) ] - tabix = TABIX_ANNOTATE.out.tbi // channel: [ val(meta), path(tbi) ] - versions = ch_versions // channel: [ path(versions.yml) ] + vcf = BCFTOOLS_ANNOTATE.out.vcf // channel: [ val(meta), path(vcf) ] + tabix = TABIX_ANNOTATE.out.tbi // channel: [ val(meta), path(tbi) ] + gvcf = DEEPVARIANT.out.gvcf // channel: [ val(meta), path(gvcf)] + gvcf_tabix = DEEPVARIANT.out.gvcf_tbi // channel: [ val(meta), path(gvcf_tbi)] + versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/subworkflows/local/variant_calling/call_snv_sentieon.nf b/subworkflows/local/variant_calling/call_snv_sentieon.nf index d0586c82..a84be1ed 100644 --- a/subworkflows/local/variant_calling/call_snv_sentieon.nf +++ b/subworkflows/local/variant_calling/call_snv_sentieon.nf @@ -47,7 +47,8 @@ workflow CALL_SNV_SENTIEON { ch_ml_model, ch_pcr_indel_model, 'VARIANT', - false) + true + ) ch_dnamodelapply_in = SENTIEON_DNASCOPE.out.vcf.join(SENTIEON_DNASCOPE.out.vcf_tbi) @@ -121,7 +122,9 @@ workflow CALL_SNV_SENTIEON { ch_versions = ch_versions.mix(TABIX_ANNOTATE.out.versions) emit: - vcf = BCFTOOLS_ANNOTATE.out.vcf // channel: [ val(meta), path(vcf) ] - tabix = TABIX_ANNOTATE.out.tbi // channel: [ val(meta), path(tbi) ] - versions = ch_versions // channel: [ path(versions.yml) ] + vcf = BCFTOOLS_ANNOTATE.out.vcf // channel: [ val(meta), path(vcf) ] + tabix = TABIX_ANNOTATE.out.tbi // channel: [ val(meta), path(tbi) ] + gvcf = SENTIEON_DNASCOPE.out.gvcf // channel: [ val(meta), path(gvcf) ] + gvcf_tbi = SENTIEON_DNASCOPE.out.gvcf_tbi // channel: [ val(meta), path(gvcf_tbi) ] + versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 342812db..b60ddad9 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -81,6 +81,10 @@ if (!params.skip_me_annotation) { mandatoryParams += ["mobile_element_svdb_annotations", "variant_consequences_snv"] } +if (!params.skip_gens) { + mandatoryParams += ["gens_gnomad_pos", "gens_interval_list", "gens_pon"] +} + for (param in mandatoryParams.unique()) { if (params[param] == null) { println("params." + param + " not set.") @@ -246,22 +250,28 @@ workflow RAREDISEASE { : ch_references.genome_bwamem2_index ch_genome_chrsizes = ch_references.genome_chrom_sizes ch_genome_fai = ch_references.genome_fai - ch_genome_dictionary = params.sequence_dictionary ? Channel.fromPath(params.sequence_dictionary).map {it -> [[id:it[0].simpleName], it]}.collect() - : ch_references.genome_dict - ch_gnomad_afidx = params.gnomad_af_idx ? Channel.fromPath(params.gnomad_af_idx).collect() - : ch_references.gnomad_af_idx - ch_gnomad_af = params.gnomad_af ? ch_gnomad_af_tab.join(ch_gnomad_afidx).map {meta, tab, idx -> [tab,idx]}.collect() - : Channel.empty() - ch_intervals_wgs = params.intervals_wgs ? Channel.fromPath(params.intervals_wgs).collect() - : Channel.empty() - ch_intervals_y = params.intervals_y ? Channel.fromPath(params.intervals_y).collect() - : Channel.empty() - ch_me_references = params.mobile_element_references ? Channel.fromSamplesheet("mobile_element_references") - : Channel.empty() - ch_me_svdb_resources = params.mobile_element_svdb_annotations ? Channel.fromPath(params.mobile_element_svdb_annotations) - : Channel.empty() - ch_ml_model = params.variant_caller.equals("sentieon") ? Channel.fromPath(params.ml_model).map {it -> [[id:it[0].simpleName], it]}.collect() - : Channel.value([[:],[]]) + ch_genome_dictionary = params.sequence_dictionary ? Channel.fromPath(params.sequence_dictionary).map {it -> [[id:it[0].simpleName], it]}.collect() + : ch_references.genome_dict + ch_gens_gnomad_pos = params.gens_gnomad_pos ? Channel.fromPath(params.gens_gnomad_pos).collect() + : Channel.empty() + ch_gens_interval_list = params.gens_interval_list ? Channel.fromPath(params.gens_interval_list).collect() + : Channel.empty() + ch_gens_pon = params.gens_pon ? Channel.fromPath(params.gens_pon).map { it -> [ [id:it[0].simpleName], it ] }.collect() + : Channel.empty() + ch_gnomad_afidx = params.gnomad_af_idx ? Channel.fromPath(params.gnomad_af_idx).collect() + : ch_references.gnomad_af_idx + ch_gnomad_af = params.gnomad_af ? ch_gnomad_af_tab.join(ch_gnomad_afidx).map {meta, tab, idx -> [tab,idx]}.collect() + : Channel.empty() + ch_intervals_wgs = params.intervals_wgs ? Channel.fromPath(params.intervals_wgs).collect() + : Channel.empty() + ch_intervals_y = params.intervals_y ? Channel.fromPath(params.intervals_y).collect() + : Channel.empty() + ch_me_references = params.mobile_element_references ? Channel.fromSamplesheet("mobile_element_references") + : Channel.empty() + ch_me_svdb_resources = params.mobile_element_svdb_annotations ? Channel.fromPath(params.mobile_element_svdb_annotations) + : Channel.empty() + ch_ml_model = params.variant_caller.equals("sentieon") ? Channel.fromPath(params.ml_model).map {it -> [[id:it[0].simpleName], it]}.collect() + : Channel.value([[:],[]]) ch_mt_intervals = ch_references.mt_intervals ch_mtshift_backchain = ch_references.mtshift_backchain ch_mtshift_bwaindex = ch_references.mtshift_bwa_index @@ -651,15 +661,15 @@ workflow RAREDISEASE { } // GENS - if (params.gens_switch) { + if ( !params.skip_gens && params.analysis_type != "wes" ) { GENS ( ch_mapped.genome_bam_bai, - CALL_SNV.out.vcf, + CALL_SNV.out.genome_gvcf, ch_genome_fasta, ch_genome_fai, - file(params.gens_interval_list), - file(params.gens_pon), - file(params.gens_gnomad_pos), + ch_gens_interval_list, + ch_gens_pon, + ch_gens_gnomad_pos, ch_case_info, ch_genome_dictionary ) From 0abbb659ff090f0abb91485585461ee787e23555 Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 13 Feb 2024 11:52:55 +0100 Subject: [PATCH 336/387] fixing linting --- workflows/raredisease.nf | 44 ++++++++++++++++++++-------------------- 1 file changed, 22 insertions(+), 22 deletions(-) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index b60ddad9..edb69b39 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -250,28 +250,28 @@ workflow RAREDISEASE { : ch_references.genome_bwamem2_index ch_genome_chrsizes = ch_references.genome_chrom_sizes ch_genome_fai = ch_references.genome_fai - ch_genome_dictionary = params.sequence_dictionary ? Channel.fromPath(params.sequence_dictionary).map {it -> [[id:it[0].simpleName], it]}.collect() - : ch_references.genome_dict - ch_gens_gnomad_pos = params.gens_gnomad_pos ? Channel.fromPath(params.gens_gnomad_pos).collect() - : Channel.empty() - ch_gens_interval_list = params.gens_interval_list ? Channel.fromPath(params.gens_interval_list).collect() - : Channel.empty() - ch_gens_pon = params.gens_pon ? Channel.fromPath(params.gens_pon).map { it -> [ [id:it[0].simpleName], it ] }.collect() - : Channel.empty() - ch_gnomad_afidx = params.gnomad_af_idx ? Channel.fromPath(params.gnomad_af_idx).collect() - : ch_references.gnomad_af_idx - ch_gnomad_af = params.gnomad_af ? ch_gnomad_af_tab.join(ch_gnomad_afidx).map {meta, tab, idx -> [tab,idx]}.collect() - : Channel.empty() - ch_intervals_wgs = params.intervals_wgs ? Channel.fromPath(params.intervals_wgs).collect() - : Channel.empty() - ch_intervals_y = params.intervals_y ? Channel.fromPath(params.intervals_y).collect() - : Channel.empty() - ch_me_references = params.mobile_element_references ? Channel.fromSamplesheet("mobile_element_references") - : Channel.empty() - ch_me_svdb_resources = params.mobile_element_svdb_annotations ? Channel.fromPath(params.mobile_element_svdb_annotations) - : Channel.empty() - ch_ml_model = params.variant_caller.equals("sentieon") ? Channel.fromPath(params.ml_model).map {it -> [[id:it[0].simpleName], it]}.collect() - : Channel.value([[:],[]]) + ch_genome_dictionary = params.sequence_dictionary ? Channel.fromPath(params.sequence_dictionary).map {it -> [[id:it[0].simpleName], it]}.collect() + : ch_references.genome_dict + ch_gens_gnomad_pos = params.gens_gnomad_pos ? Channel.fromPath(params.gens_gnomad_pos).collect() + : Channel.empty() + ch_gens_interval_list = params.gens_interval_list ? Channel.fromPath(params.gens_interval_list).collect() + : Channel.empty() + ch_gens_pon = params.gens_pon ? Channel.fromPath(params.gens_pon).map { it -> [ [id:it[0].simpleName], it ] }.collect() + : Channel.empty() + ch_gnomad_afidx = params.gnomad_af_idx ? Channel.fromPath(params.gnomad_af_idx).collect() + : ch_references.gnomad_af_idx + ch_gnomad_af = params.gnomad_af ? ch_gnomad_af_tab.join(ch_gnomad_afidx).map {meta, tab, idx -> [tab,idx]}.collect() + : Channel.empty() + ch_intervals_wgs = params.intervals_wgs ? Channel.fromPath(params.intervals_wgs).collect() + : Channel.empty() + ch_intervals_y = params.intervals_y ? Channel.fromPath(params.intervals_y).collect() + : Channel.empty() + ch_me_references = params.mobile_element_references ? Channel.fromSamplesheet("mobile_element_references") + : Channel.empty() + ch_me_svdb_resources = params.mobile_element_svdb_annotations ? Channel.fromPath(params.mobile_element_svdb_annotations) + : Channel.empty() + ch_ml_model = params.variant_caller.equals("sentieon") ? Channel.fromPath(params.ml_model).map {it -> [[id:it[0].simpleName], it]}.collect() + : Channel.value([[:],[]]) ch_mt_intervals = ch_references.mt_intervals ch_mtshift_backchain = ch_references.mtshift_backchain ch_mtshift_bwaindex = ch_references.mtshift_bwa_index From 12c538209770b60532009593b7ce664764a5384a Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 13 Feb 2024 17:09:12 +0100 Subject: [PATCH 337/387] splited gens_pon in gens_pon_male and gens_pon_female --- CHANGELOG.md | 3 ++- main.nf | 3 ++- nextflow_schema.json | 15 ++++++++++++--- subworkflows/local/gens.nf | 36 +++++++++++++++++++++++++++--------- workflows/raredisease.nf | 9 ++++++--- 5 files changed, 49 insertions(+), 17 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 7c0256e2..3d852b52 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -64,10 +64,11 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Updated modules from nf-core [#412](https://github.com/nf-core/raredisease/pull/412) - If present, remove duplicate entries in probands and upd_children in the meta. [#420](https://github.com/nf-core/raredisease/pull/420) - Fixes vep starting as many instances as the square of the number of scatters. [#405](https://github.com/nf-core/raredisease/pull/405) -- Replaced the logic where we added an arbitrary substring to keep file names unique after alignment which we then removed using a split operator, with a simple copy operation. [#425](https://github.com/nf-core/raredisease/pull/425/files) +- Replaced the logic where we added an arbitrary substring to keep file names unique after alignment which we then removed using a split operator, with a simple copy operation. [#425](https://github.com/nf-core/raredisease/pull/425) - Preventing a crash of rhocall annotate in the case of running four individuals whereof two are affected. - Fixed memory qualifier in gatk4 germlinecnvcaller and postprocessgermlinecnvcalls - Fixed wrong process names when outputting versions in `ALIGN_SENTIEON` and `CALL_SNV`. +- Fixed gens subworkflow [#515](https://github.com/nf-core/raredisease/pull/515) ### `Updated` diff --git a/main.nf b/main.nf index 5327e44e..9b19e359 100644 --- a/main.nf +++ b/main.nf @@ -24,7 +24,8 @@ params.call_interval = WorkflowMain.getGenomeAttribute(params, params.cadd_resources = WorkflowMain.getGenomeAttribute(params, 'cadd_resources') params.gcnvcaller_model = WorkflowMain.getGenomeAttribute(params, 'gcnvcaller_model') params.gens_interval_list = WorkflowMain.getGenomeAttribute(params, 'gens_interval_list') -params.gens_pon = WorkflowMain.getGenomeAttribute(params, 'gens_pon') +params.gens_pon_female = WorkflowMain.getGenomeAttribute(params, 'gens_pon_female') +params.gens_pon_male = WorkflowMain.getGenomeAttribute(params, 'gens_pon_male') params.gens_gnomad_pos = WorkflowMain.getGenomeAttribute(params, 'gens_gnomad_pos') params.gnomad_af = WorkflowMain.getGenomeAttribute(params, 'gnomad_af') params.gnomad_af_idx = WorkflowMain.getGenomeAttribute(params, 'gnomad_af_idx') diff --git a/nextflow_schema.json b/nextflow_schema.json index 1ea97d44..03ab1c6a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -135,13 +135,22 @@ "help_text": "This file contains the binning intervals used for CollectReadCounts.", "hidden": true }, - "gens_pon": { + "gens_pon_female": { "type": "string", "exists": true, "format": "file-path", "fa_icon": "fas fa-file", - "description": "Path to panel of normals for Gens.", - "help_text": "The panel used to run DenoiseReadCounts.", + "description": "Path to female panel of normals for Gens.", + "help_text": "The female panel used to run DenoiseReadCounts.", + "hidden": true + }, + "gens_pon_male": { + "type": "string", + "exists": true, + "format": "file-path", + "fa_icon": "fas fa-file", + "description": "Path to male panel of normals for Gens.", + "help_text": "The male panel used to run DenoiseReadCounts.", "hidden": true }, "gnomad_af": { diff --git a/subworkflows/local/gens.nf b/subworkflows/local/gens.nf index 30f94cc3..e9b86360 100644 --- a/subworkflows/local/gens.nf +++ b/subworkflows/local/gens.nf @@ -2,9 +2,10 @@ // A preprocessing workflow for Gens // -include { GATK4_COLLECTREADCOUNTS as COLLECTREADCOUNTS } from '../../modules/nf-core/gatk4/collectreadcounts/main' -include { GATK4_DENOISEREADCOUNTS as DENOISEREADCOUNTS } from '../../modules/nf-core/gatk4/denoisereadcounts/main' -include { GENS as GENS_GENERATE } from '../../modules/local/gens/main' +include { GATK4_COLLECTREADCOUNTS as COLLECTREADCOUNTS } from '../../modules/nf-core/gatk4/collectreadcounts/main' +include { GATK4_DENOISEREADCOUNTS as DENOISEREADCOUNTS_FEMALE } from '../../modules/nf-core/gatk4/denoisereadcounts/main' +include { GATK4_DENOISEREADCOUNTS as DENOISEREADCOUNTS_MALE } from '../../modules/nf-core/gatk4/denoisereadcounts/main' +include { GENS as GENS_GENERATE } from '../../modules/local/gens/main' workflow GENS { take: @@ -13,7 +14,8 @@ workflow GENS { ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] ch_interval_list // channel: [mandatory] [ path(interval_list) ] - ch_pon // channel: [mandatory] [ path(pon) ] + ch_pon_female // channel: [mandatory] [ path(pon) ] + ch_pon_male // channel: [mandatory] [ path(pon) ] ch_gnomad_pos // channel: [mandatory] [ path(gnomad_pos) ] ch_case_info // channel: [mandatory] [ val(case_info) ] ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] @@ -32,19 +34,35 @@ workflow GENS { ch_genome_dictionary ) - DENOISEREADCOUNTS ( - COLLECTREADCOUNTS.out.hdf5, - ch_pon + COLLECTREADCOUNTS.out.hdf5 + .branch { meta, counts -> + female: meta.sex.equals(2) || meta.sex.equals(0) + male: meta.sex.equals(1) + } + .set { ch_denoisereadcounts_in } + + DENOISEREADCOUNTS_FEMALE ( + ch_denoisereadcounts_in.female, + ch_pon_female + ) + + DENOISEREADCOUNTS_MALE ( + ch_denoisereadcounts_in.male, + ch_pon_male ) + DENOISEREADCOUNTS_FEMALE.out.standardized + .mix(DENOISEREADCOUNTS_MALE.out.standardized) + .set { ch_denoisereadcounts_out } GENS_GENERATE ( - DENOISEREADCOUNTS.out.standardized, + ch_denoisereadcounts_out, ch_gvcf, ch_gnomad_pos ) ch_versions = ch_versions.mix(COLLECTREADCOUNTS.out.versions.first()) - ch_versions = ch_versions.mix(DENOISEREADCOUNTS.out.versions.first()) + ch_versions = ch_versions.mix(DENOISEREADCOUNTS_FEMALE.out.versions.first()) + ch_versions = ch_versions.mix(DENOISEREADCOUNTS_MALE.out.versions.first()) ch_versions = ch_versions.mix(GENS_GENERATE.out.versions.first()) emit: diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index edb69b39..dcac59c9 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -82,7 +82,7 @@ if (!params.skip_me_annotation) { } if (!params.skip_gens) { - mandatoryParams += ["gens_gnomad_pos", "gens_interval_list", "gens_pon"] + mandatoryParams += ["gens_gnomad_pos", "gens_interval_list", "gens_pon_female", "gens_pon_male"] } for (param in mandatoryParams.unique()) { @@ -256,7 +256,9 @@ workflow RAREDISEASE { : Channel.empty() ch_gens_interval_list = params.gens_interval_list ? Channel.fromPath(params.gens_interval_list).collect() : Channel.empty() - ch_gens_pon = params.gens_pon ? Channel.fromPath(params.gens_pon).map { it -> [ [id:it[0].simpleName], it ] }.collect() + ch_gens_pon_female = params.gens_pon_female ? Channel.fromPath(params.gens_pon_female).map { it -> [ [id:it[0].simpleName], it ] }.collect() + : Channel.empty() + ch_gens_pon_male = params.gens_pon_male ? Channel.fromPath(params.gens_pon_male).map { it -> [ [id:it[0].simpleName], it ] }.collect() : Channel.empty() ch_gnomad_afidx = params.gnomad_af_idx ? Channel.fromPath(params.gnomad_af_idx).collect() : ch_references.gnomad_af_idx @@ -668,7 +670,8 @@ workflow RAREDISEASE { ch_genome_fasta, ch_genome_fai, ch_gens_interval_list, - ch_gens_pon, + ch_gens_pon_female, + ch_gens_pon_male, ch_gens_gnomad_pos, ch_case_info, ch_genome_dictionary From 63bcbc117c07ce01727b8c81d28b592038cbc77d Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 13 Feb 2024 17:32:02 +0100 Subject: [PATCH 338/387] fix publishing of gens outfiles --- conf/modules/gens.config | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/conf/modules/gens.config b/conf/modules/gens.config index fdd798f5..6882775c 100644 --- a/conf/modules/gens.config +++ b/conf/modules/gens.config @@ -30,7 +30,8 @@ process { ].join(' ') } } - withName: '.*GENS:GENERATE_DATA' { + withName: '.*GENS:GENS_GENERATE' { + ext.prefix = { "${meta.id}_gens" } publishDir = [ path: { "${params.outdir}/gens" }, mode: params.publish_dir_mode, From a91c4b421f11ef46a5266d9d140cba99e77c3711 Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 13 Feb 2024 17:41:37 +0100 Subject: [PATCH 339/387] Add error message when trying to run gens with conda --- modules/local/gens/main.nf | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/modules/local/gens/main.nf b/modules/local/gens/main.nf index 6708e79c..33731814 100644 --- a/modules/local/gens/main.nf +++ b/modules/local/gens/main.nf @@ -17,6 +17,10 @@ process GENS { path "versions.yml" , emit: versions script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "The gens pre-processing module does not support Conda. Please use Docker / Singularity / Podman instead." + } def prefix = task.ext.prefix ?: "${meta.id}" """ generate_gens_data.pl \\ From 8e0935e3df6859f83e507df85bb3f97951777c07 Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 13 Feb 2024 18:24:04 +0100 Subject: [PATCH 340/387] add usage --- docs/usage.md | 16 ++++++++++++++++ 1 file changed, 16 insertions(+) diff --git a/docs/usage.md b/docs/usage.md index b1ec69cd..878fe121 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -23,6 +23,7 @@ Table of contents: - [9. Mitochondrial annotation](#9-mitochondrial-annotation) - [10. Mobile element annoation](#10-mobile-element-annotation) - [11. Variant evaluation](#11-variant-evaluation) + - [12. Prepare data CNV visualization in Gens](#12-prepare-data-for-cnv-visualisation-in-gens) - [Run the pipeline](#run-the-pipeline) - [Direct input in CLI](#direct-input-in-cli) - [Import from a config file (recommended)](#import-from-a-config-file-recommended) @@ -298,6 +299,21 @@ no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sampl 1 This parameter is set to false by default, set it to true if if you'd like to run the evaluation subworkflow 2 A CSV file that describes the truth VCF files used by RTG Tools' vcfeval for evaluating SNVs. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/rtg_example.csv). The file contains four columns `samplename,vcf,bedregions,evaluationregions` where samplename is the user assigned samplename in the input samplesheet, vcf is the path to the truth vcf file, bedregions and evaluationregions are the path to the bed files that are supposed to be passed through --bed_regions and --evaluation_regions options of vcfeval. +##### 12. Prepare data for CNV visualisation in Gens + +Optionally the read data can be prepared for CNV visualization in [Gens](https://github.com/Clinical-Genomics-Lund/gens). This subworkflow is turned off by default. You can activate it by supplying the option `--skip_gens false`. + +| Mandatory | Optional | +| ------------------------------ | -------- | +| gens_pon_female1 | | +| gens_pon_male1 | | +| gens_interval_list2 | | +| gens_gnomad_pos3 | | + +1 Instructions on how to generate the panel of normals can be found [here](https://github.com/Clinical-Genomics-Lund/gens?tab=readme-ov-file#create-pon)
+2 Interval list for CollectReadCounts. Instructions on how to generate the interval list file can be found [here](https://github.com/Clinical-Genomics-Lund/gens?tab=readme-ov-file#create-pon)
+3 File containing SNVs to be used for the B-allele frequency calculations. The developers of gens uses SNVs in gnomad with an allele frecuency above 5%. + #### Run the pipeline You can directly supply the parameters in the command line (CLI) or use a config file from which the pipeline can import the parameters. From 8812ea7a084f9eca8c9db50356c26847f59e1fdc Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 13 Feb 2024 18:33:12 +0100 Subject: [PATCH 341/387] adding gens output description --- docs/output.md | 17 +++++++++++++++++ 1 file changed, 17 insertions(+) diff --git a/docs/output.md b/docs/output.md index 2f782cf4..448322da 100644 --- a/docs/output.md +++ b/docs/output.md @@ -72,6 +72,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Calling mobile elements](#calling-mobile-elements) - [Annotating mobile elements](#annotating-mobile-elements) - [Variant evaluation](#variant-evaluation) + - [Gens](#gens) - [Pipeline information](#pipeline-information) ### Alignment @@ -594,6 +595,22 @@ Provided a truth set, SNVs can be evaluated using RTG Tools' vcfeval engine. Out
+### Gens + +The sequencing data can be prepared for visualization of CNVs in [Gens](https://github.com/Clinical-Genomics-Lund/gens). This subworkflow is turned off by default. You can activate it by supplying the option `--skip_gens false`. You can read more about how to setup Gens [here](https://github.com/Clinical-Genomics-Lund/gens). + +
+Output files + +- `gens/` + + - `_gens.baf.bed.gz`: contains sample b-allele frequencies in bed format. + - `_gens.baf.bed.gz.tbi`: index of the \*baf.bed.gz file. + - `_gens.cov.bed.gz`: contains sample coverage in bed format. + - `_gens.cov.bed.gz.tbi`: index of the \*cov.bed.gz file. + +
+ ### Pipeline information [Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. From 0793a2c56a6cfffa4c8b115e3695dd33a5e76e3c Mon Sep 17 00:00:00 2001 From: jemten Date: Tue, 13 Feb 2024 18:37:24 +0100 Subject: [PATCH 342/387] add citation --- CITATIONS.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/CITATIONS.md b/CITATIONS.md index 1fb40da9..36b3cd7b 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -60,6 +60,8 @@ > Magnusson M, Hughes T, Glabilloy, Bitdeli Chef. genmod: Version 3.7.3. Published online November 15, 2018. doi:10.5281/ZENODO.3841142 +- [Gens](https://github.com/Clinical-Genomics-Lund/gens) + - [GLnexus](https://academic.oup.com/bioinformatics/article/36/24/5582/6064144) > Yun T, Li H, Chang PC, Lin MF, Carroll A, McLean CY. Accurate, scalable cohort variant calls using DeepVariant and GLnexus. Robinson P, ed. Bioinformatics. 2021;36(24):5582-5589. doi:10.1093/bioinformatics/btaa1081 From 48cf0b30b516a29949cca1e21d29579d23ab8075 Mon Sep 17 00:00:00 2001 From: Anders Jemt Date: Wed, 14 Feb 2024 13:35:53 +0100 Subject: [PATCH 343/387] aligning comments --- subworkflows/local/gens.nf | 18 +++++++++--------- 1 file changed, 9 insertions(+), 9 deletions(-) diff --git a/subworkflows/local/gens.nf b/subworkflows/local/gens.nf index e9b86360..93f1ac0a 100644 --- a/subworkflows/local/gens.nf +++ b/subworkflows/local/gens.nf @@ -9,16 +9,16 @@ include { GENS as GENS_GENERATE } from '../../modu workflow GENS { take: - ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] - ch_gvcf // channel: [mandatory] [ val(meta), path(gvcf) ] - ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] - ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] - ch_interval_list // channel: [mandatory] [ path(interval_list) ] + ch_bam_bai // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + ch_gvcf // channel: [mandatory] [ val(meta), path(gvcf) ] + ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] + ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] + ch_interval_list // channel: [mandatory] [ path(interval_list) ] ch_pon_female // channel: [mandatory] [ path(pon) ] - ch_pon_male // channel: [mandatory] [ path(pon) ] - ch_gnomad_pos // channel: [mandatory] [ path(gnomad_pos) ] - ch_case_info // channel: [mandatory] [ val(case_info) ] - ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] + ch_pon_male // channel: [mandatory] [ path(pon) ] + ch_gnomad_pos // channel: [mandatory] [ path(gnomad_pos) ] + ch_case_info // channel: [mandatory] [ val(case_info) ] + ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] main: ch_versions = Channel.empty() From b4808d5913d8f05da502545ae24d1ce694a9096f Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 14 Feb 2024 16:43:22 +0100 Subject: [PATCH 344/387] standardized output file names --- conf/modules/annotate_genome_snvs.config | 27 +++--- conf/modules/annotate_mt_snvs.config | 85 ++++++++++--------- .../annotate_structural_variants.config | 2 +- conf/modules/call_structural_variants.config | 1 + conf/modules/generate_clinical_set.config | 17 +--- conf/modules/qc_bam.config | 1 + conf/modules/rank_variants.config | 4 +- docs/output.md | 20 ++--- modules.json | 7 +- modules/nf-core/gatk4/selectvariants/main.nf | 12 +-- .../gatk4/selectvariants/tests/main.nf.test | 64 ++++++++++++++ .../gatk4/selectvariants/tests/tags.yml | 2 + .../hmtnote/annotate/hmtnote-annotate.diff | 42 +++++++++ modules/nf-core/hmtnote/annotate/main.nf | 10 +-- modules/nf-core/svdb/merge/main.nf | 8 +- modules/nf-core/svdb/merge/tests/main.nf.test | 57 +++++++++++++ modules/nf-core/svdb/merge/tests/tags.yml | 2 + subworkflows/local/annotate_genome_snvs.nf | 12 ++- subworkflows/local/annotate_mt_snvs.nf | 17 ++-- .../local/annotate_structural_variants.nf | 4 +- 20 files changed, 284 insertions(+), 110 deletions(-) create mode 100644 modules/nf-core/gatk4/selectvariants/tests/main.nf.test create mode 100644 modules/nf-core/gatk4/selectvariants/tests/tags.yml create mode 100644 modules/nf-core/hmtnote/annotate/hmtnote-annotate.diff create mode 100644 modules/nf-core/svdb/merge/tests/main.nf.test create mode 100644 modules/nf-core/svdb/merge/tests/tags.yml diff --git a/conf/modules/annotate_genome_snvs.config b/conf/modules/annotate_genome_snvs.config index bf748350..768aeee3 100644 --- a/conf/modules/annotate_genome_snvs.config +++ b/conf/modules/annotate_genome_snvs.config @@ -25,26 +25,29 @@ process { withName: '.*ANNOTATE_GENOME_SNVS:RHOCALL_ANNOTATE' { ext.args = { "--v14 " } - ext.prefix = { "${meta.id}_rohann" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:ZIP_TABIX_ROHCALL' { + ext.prefix = { "${input.simpleName}" } } withName: '.*ANNOTATE_GENOME_SNVS:VCFANNO' { - ext.prefix = { "${meta.id}_rohann_vcfanno" } + ext.prefix = { "${vcf.simpleName}_vcfanno" } } withName: '.*ANNOTATE_GENOME_SNVS:UPD_SITES' { - ext.prefix = { "${meta.id}_rohann_vcfanno_upd_sites" } + ext.prefix = { "${vcf.simpleName}_upd_sites" } ext.args = {"--af-tag GNOMADAF --proband ${meta.upd_child} --mother ${meta.mother} --father ${meta.father} sites"} } withName: '.*ANNOTATE_GENOME_SNVS:UPD_REGIONS' { - ext.prefix = { "${meta.id}_rohann_vcfanno_upd_regions" } + ext.prefix = { "${vcf.simpleName}_upd_regions" } ext.args = {"--af-tag GNOMADAF --proband ${meta.upd_child} --mother ${meta.mother} --father ${meta.father} regions --min-size 5 --min-sites 1"} ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun } } withName: '.*ANNOTATE_GENOME_SNVS:CHROMOGRAPH_SITES' { - ext.prefix = { "${meta7.id}_rohann_vcfanno_upd_sites_chromograph" } + ext.prefix = { "${sites.simpleName}_chromograph" } ext.args = { "--euploid" } tag = {"${meta7.id}"} publishDir = [ @@ -55,7 +58,7 @@ process { } withName: '.*ANNOTATE_GENOME_SNVS:CHROMOGRAPH_REGIONS' { - ext.prefix = { "${meta6.id}_rohann_vcfanno_upd_regions_chromograph" } + ext.prefix = { "${regions.simpleName}_chromograph" } ext.args = { '--euploid' } ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample', 'test_sentieon']).size() >= 1) || workflow.stubRun } tag = {"${meta6.id}"} @@ -66,17 +69,21 @@ process { ] } + withName: '.*ANNOTATE_GENOME_SNVS:ZIP_TABIX_VCFANNO' { + ext.prefix = { "${input.simpleName}" } + } + withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_VIEW' { - ext.prefix = { "${meta.id}_rohann_vcfanno_filter" } + ext.prefix = { "${vcf.simpleName}_filter" } ext.args = { '--output-type z --exclude "INFO/GNOMADAF > 0.70 | INFO/GNOMADAF_popmax > 0.70" ' } } withName: '.*ANNOTATE_GENOME_SNVS:GATK4_SELECTVARIANTS' { - ext.prefix = { "${meta.id}_${intervals.simpleName}" } + ext.prefix = { "${vcf.simpleName}_${intervals.simpleName}" } } withName: '.*ANNOTATE_GENOME_SNVS:ENSEMBLVEP_SNV' { - ext.prefix = { "${vcf.simpleName}_rohann_vcfanno_filter_vep" } + ext.prefix = { "${meta.prefix}_vep_${meta.scatterid}" } ext.args = { [ '--dir_plugins vep_cache/Plugins', '--plugin LoFtool,LoFtool_scores.txt', @@ -94,7 +101,7 @@ process { } withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_CONCAT' { - ext.prefix = { "${meta.id}_rohann_vcfanno_filter_vep" } + ext.prefix = { "${meta.prefix}_vep" } publishDir = [ path: { "${params.outdir}/annotate_snv/genome" }, mode: params.publish_dir_mode, diff --git a/conf/modules/annotate_mt_snvs.config b/conf/modules/annotate_mt_snvs.config index 45260fb2..3e25c36f 100644 --- a/conf/modules/annotate_mt_snvs.config +++ b/conf/modules/annotate_mt_snvs.config @@ -16,49 +16,52 @@ // process { - if (!params.skip_mt_annotation) { - withName: '.*ANNOTATE_MT_SNVS:ENSEMBLVEP_MT' { - ext.args = { [ - '--dir_plugins vep_cache/Plugins', - '--plugin LoFtool,LoFtool_scores.txt', - '--plugin pLI,pLI_values.txt', - '--plugin SpliceAI,snv=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', - '--distance 0', - '--buffer_size 20000', - '--format vcf --fork 4 --max_sv_size 248956422', - '--appris --biotype --cache --canonical --ccds --compress_output bgzip', - '--domains --exclude_predicted --force_overwrite', - '--hgvs --humdiv --no_progress --numbers', - '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl --vcf', - '--uniprot' - ].join(' ') } - } + withName: '.*ANNOTATE_MT_SNVS:ENSEMBLVEP_MT' { + ext.args = { [ + '--dir_plugins vep_cache/Plugins', + '--plugin LoFtool,LoFtool_scores.txt', + '--plugin pLI,pLI_values.txt', + '--plugin SpliceAI,snv=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', + '--distance 0', + '--buffer_size 20000', + '--format vcf --fork 4 --max_sv_size 248956422', + '--appris --biotype --cache --canonical --ccds --compress_output bgzip', + '--domains --exclude_predicted --force_overwrite', + '--hgvs --humdiv --no_progress --numbers', + '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl --vcf', + '--uniprot' + ].join(' ') } + ext.prefix = { "${meta.prefix}" } + } - withName: '.*ANNOTATE_MT_SNVS:ZIP_TABIX_HMTNOTE' { - ext.prefix = { "${meta.id}_vep_vcfanno_hmtnote_mt" } - publishDir = [ - path: { "${params.outdir}/annotate_snv/mitochondria" }, - mode: params.publish_dir_mode, - pattern: "*{vcf.gz,vcf.gz.tbi}", - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*ANNOTATE_MT_SNVS:VCFANNO_MT' { + ext.prefix = { "${meta.prefix}" } + } - withName: '.*ANNOTATE_MT_SNVS:HMTNOTE_ANNOTATE' { - ext.prefix = { "${meta.id}_vep_vcfanno_hmtnote_mt" } - ext.args = '--offline' - publishDir = [ - enabled: false - ] - } + withName: '.*ANNOTATE_MT_SNVS:ZIP_TABIX_HMTNOTE' { + ext.prefix = { "${meta.prefix}" } + publishDir = [ + path: { "${params.outdir}/annotate_snv/mitochondria" }, + mode: params.publish_dir_mode, + pattern: "*{vcf.gz,vcf.gz.tbi}", + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*ANNOTATE_MT_SNVS:HMTNOTE_ANNOTATE' { + ext.prefix = { "${meta.prefix}_uncompressed" } + ext.args = '--offline' + publishDir = [ + enabled: false + ] + } - withName: '.*ANNOTATE_MT_SNVS:HAPLOGREP2_CLASSIFY_MT' { - ext.prefix = { "${meta.id}_haplogrep" } - publishDir = [ - path: { "${params.outdir}/annotate_snv/mitochondria" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*ANNOTATE_MT_SNVS:HAPLOGREP2_CLASSIFY_MT' { + ext.prefix = { "${meta.prefix}_haplogrep" } + publishDir = [ + path: { "${params.outdir}/annotate_snv/mitochondria" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } } diff --git a/conf/modules/annotate_structural_variants.config b/conf/modules/annotate_structural_variants.config index 62faeda7..f8666a82 100644 --- a/conf/modules/annotate_structural_variants.config +++ b/conf/modules/annotate_structural_variants.config @@ -35,7 +35,7 @@ process { } withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:PICARD_SORTVCF' { - ext.prefix = { "${meta.id}_sortvcf" } + ext.prefix = { "${meta.id}_svdbquery" } } withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:BCFTOOLS_VIEW' { diff --git a/conf/modules/call_structural_variants.config b/conf/modules/call_structural_variants.config index 3a59868d..f8da7840 100644 --- a/conf/modules/call_structural_variants.config +++ b/conf/modules/call_structural_variants.config @@ -24,6 +24,7 @@ process { } withName: '.*CALL_STRUCTURAL_VARIANTS:SVDB_MERGE' { + ext.prefix = {"${meta.id}_sv"} ext.args = '--pass_only --same_order' publishDir = [ path: { "${params.outdir}/call_sv/genome" }, diff --git a/conf/modules/generate_clinical_set.config b/conf/modules/generate_clinical_set.config index 794e284b..4ab31579 100644 --- a/conf/modules/generate_clinical_set.config +++ b/conf/modules/generate_clinical_set.config @@ -27,11 +27,6 @@ process { withName: '.*:GENERATE_CLINICAL_SET_SNV:TABIX_BGZIP' { ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_snv_${meta.set}" } - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] } } @@ -40,18 +35,13 @@ process { process { withName: '.*:GENERATE_CLINICAL_SET_SV:ENSEMBLVEP_FILTERVEP' { ext.when = !params.skip_vep_filter - ext.prefix = { "${meta.id}_sv_${meta.set}" } + ext.prefix = { "${input.simpleName}_sv_${meta.set}" } ext.args = { "--filter \"HGNC_ID in ${meta.hgnc_ids.join(',')}\"" } } withName: '.*:GENERATE_CLINICAL_SET_SV:TABIX_BGZIP' { ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_sv_${meta.set}" } - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] } } @@ -67,10 +57,5 @@ process { withName: '.*:GENERATE_CLINICAL_SET_MT:TABIX_BGZIP' { ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_mt_${meta.set}" } - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] } } diff --git a/conf/modules/qc_bam.config b/conf/modules/qc_bam.config index 93b0f2db..d57670c7 100644 --- a/conf/modules/qc_bam.config +++ b/conf/modules/qc_bam.config @@ -48,6 +48,7 @@ process { withName: '.*QC_BAM:UCSC_WIGTOBIGWIG' { ext.args = '-clip' + ext.prefix = { "${meta.id}_tidditcov" } } withName: '.*QC_BAM:CHROMOGRAPH_COV' { diff --git a/conf/modules/rank_variants.config b/conf/modules/rank_variants.config index b65d55ab..a17dee05 100644 --- a/conf/modules/rank_variants.config +++ b/conf/modules/rank_variants.config @@ -31,8 +31,8 @@ process { ].join(' ') } } - withName: '.*RANK_VARIANTS_SNV:GENMOD_MODELS' { - ext.prefix = { "${meta.id}_snv_genmod_models_${meta.set}" } + withName: '.*RANK_VARIANTS_SV:GENMOD_MODELS' { + ext.prefix = { "${meta.id}_sv_genmod_models_${meta.set}" } ext.args = " --whole_gene " } diff --git a/docs/output.md b/docs/output.md index 4edd4376..0df151b9 100644 --- a/docs/output.md +++ b/docs/output.md @@ -265,8 +265,8 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ Output files - `call_snv/genome` - - `_nomito.selectvariants.vcf.gz`: normalized vcf file containing no MT variants. - - `_nomito.selectvariants.vcf.gz.tbi`: index of the vcf file containing no MT variants. + - `_nomito.vcf.gz`: normalized vcf file containing no MT variants. + - `_nomito.vcf.gz.tbi`: index of the vcf file containing no MT variants. @@ -278,8 +278,8 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support. Output files - `call_snv/genome` - - `_nomito.selectvariants.vcf.gz`: normalized vcf file containing no MT variants. - - `_nomito.selectvariants.vcf.gz.tbi`: index of the vcf file containing no MT variants. + - `_nomito.vcf.gz`: normalized vcf file containing no MT variants. + - `_nomito.vcf.gz.tbi`: index of the vcf file containing no MT variants. @@ -381,8 +381,8 @@ Based on VEP annotations, custom scripts used by the pipeline further annotate e Output files - `annotate_snv/genome` - - `_rohann_vcfanno_filter_vep.vcf.gz`: file containing bcftools roh, vcfanno, and vep annotations. - - `_rohann_vcfanno_filter_vep.vcf.gz.tbi`: index of the file containing bcftools roh, vcfanno, and vep annotations. + - `_rhocall_vcfanno_filter_.vcf.gz`: file containing bcftools roh, vcfanno, cadd and vep annotations. + - `_rhocall_vcfanno_filter_.vcf.gz.tbi`: index of the file containing bcftools roh, vcfanno, cadd and vep annotations. @@ -455,7 +455,7 @@ The pipeline for mitochondrial variant discovery, using Mutect2, uses a high sen [MT deletion script](https://github.com/dnil/mitosign/blob/master/run_mt_del_check.sh) lists the fraction of mitochondrially aligning read pairs (per 1000) that appear discordant, as defined by an insert size of more than 1.2 kb (and less than 15 kb due to the circular nature of the genome) using samtools. - `call_sv/mitochondria` - - `.txt`: file containing deletions. + - `_mt_del.txt`: file containing deletions. ##### eKLIPse @@ -476,7 +476,7 @@ The pipeline for mitochondrial variant discovery, using Mutect2, uses a high sen Output files - `annotate_snv/mitochondria` - - `_haplogrep.txt`: file containing haplogroup information. + - `_mitochondria_haplogrep.txt`: file containing haplogroup information. @@ -502,8 +502,8 @@ We recommend using vcfanno to annotate SNVs with precomputed CADD scores (files Output files - `annotate_snv/mitochondria` - - `_vep_vcfanno_hmtnote_mt.vcf.gz`: file containing mitochondrial annotations. - - `_vep_vcfanno_hmtnote_mt.vcf.gz.tbi`: index of the file containing mitochondrial annotations. + - `_mitochondria_vep_vcfanno_hmtnote.vcf.gz`: file containing mitochondrial annotations. + - `_mitochondria_vep_vcfanno_hmtnote.vcf.gz.tbi`: index of the file containing mitochondrial annotations. diff --git a/modules.json b/modules.json index e98107a0..cf999956 100644 --- a/modules.json +++ b/modules.json @@ -225,7 +225,7 @@ }, "gatk4/selectvariants": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "47cd015ac64f0294ae65a589693604127132a686", "installed_by": ["modules"] }, "gatk4/shiftfasta": { @@ -281,7 +281,8 @@ "hmtnote/annotate": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/hmtnote/annotate/hmtnote-annotate.diff" }, "manta/germline": { "branch": "master", @@ -455,7 +456,7 @@ }, "svdb/merge": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d9c95c45db561814ff6c46a0c0574581e81beaae", "installed_by": ["modules"] }, "svdb/query": { diff --git a/modules/nf-core/gatk4/selectvariants/main.nf b/modules/nf-core/gatk4/selectvariants/main.nf index f81a019b..139d7835 100644 --- a/modules/nf-core/gatk4/selectvariants/main.nf +++ b/modules/nf-core/gatk4/selectvariants/main.nf @@ -11,9 +11,9 @@ process GATK4_SELECTVARIANTS { tuple val(meta), path(vcf), path(vcf_idx), path (intervals) output: - tuple val(meta), path("*.selectvariants.vcf.gz") , emit: vcf - tuple val(meta), path("*.selectvariants.vcf.gz.tbi") , emit: tbi - path "versions.yml" , emit: versions + tuple val(meta), path("*.vcf.gz") , emit: vcf + tuple val(meta), path("*.vcf.gz.tbi") , emit: tbi + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -33,7 +33,7 @@ process GATK4_SELECTVARIANTS { gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ SelectVariants \\ --variant $vcf \\ - --output ${prefix}.selectvariants.vcf.gz \\ + --output ${prefix}.vcf.gz \\ $interval \\ --tmp-dir . \\ $args @@ -47,8 +47,8 @@ process GATK4_SELECTVARIANTS { stub: def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${prefix}.selectvariants.vcf.gz - touch ${prefix}.selectvariants.vcf.gz.tbi + touch ${prefix}.vcf.gz + touch ${prefix}.vcf.gz.tbi cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/gatk4/selectvariants/tests/main.nf.test b/modules/nf-core/gatk4/selectvariants/tests/main.nf.test new file mode 100644 index 00000000..d1622dad --- /dev/null +++ b/modules/nf-core/gatk4/selectvariants/tests/main.nf.test @@ -0,0 +1,64 @@ +nextflow_process { + + name "Test Process GATK4_SELECTVARIANTS" + script "modules/nf-core/gatk4/selectvariants/main.nf" + process "GATK4_SELECTVARIANTS" + tag "modules" + tag "modules_nfcore" + tag "gatk4" + tag "gatk4/selectvariants" + + test("selectvariants - vcf input") { + + when { + params { + // define parameters here. Example: + // outdir = "tests/results" + } + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_genome_vcf'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_genome_vcf_idx'], checkIfExists: true), + file(params.test_data['homo_sapiens']['genome']['genome_interval_list'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert path(process.out.vcf.get(0).get(1)).linesGzip.contains("##fileformat=VCFv4.2")} + ) + } + + + } + + test("selectvariants - gz input") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_genome_vcf_gz'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_genome_vcf_gz_tbi'], checkIfExists: true), + [] + ] + """ + } + } + + then { + assertAll( + {assert process.success}, + {assert path(process.out.vcf.get(0).get(1)).linesGzip.contains("##fileformat=VCFv4.2")} + ) + } + + } + +} diff --git a/modules/nf-core/gatk4/selectvariants/tests/tags.yml b/modules/nf-core/gatk4/selectvariants/tests/tags.yml new file mode 100644 index 00000000..d9fb50f7 --- /dev/null +++ b/modules/nf-core/gatk4/selectvariants/tests/tags.yml @@ -0,0 +1,2 @@ +gatk4/selectvariants: + - "modules/nf-core/gatk4/selectvariants/**" diff --git a/modules/nf-core/hmtnote/annotate/hmtnote-annotate.diff b/modules/nf-core/hmtnote/annotate/hmtnote-annotate.diff new file mode 100644 index 00000000..3723cf2f --- /dev/null +++ b/modules/nf-core/hmtnote/annotate/hmtnote-annotate.diff @@ -0,0 +1,42 @@ +Changes in module 'nf-core/hmtnote/annotate' +--- modules/nf-core/hmtnote/annotate/main.nf ++++ modules/nf-core/hmtnote/annotate/main.nf +@@ -11,7 +11,7 @@ + tuple val(meta), path(vcf) + + output: +- tuple val(meta), path("*_annotated.vcf"), emit: vcf ++ tuple val(meta), path("${prefix}.vcf"), emit: vcf + path "versions.yml" , emit: versions + + when: +@@ -19,13 +19,13 @@ + + script: + def args = task.ext.args ?: '' +- def prefix = task.ext.prefix ?: "${meta.id}" ++ prefix = task.ext.prefix ?: "${meta.id}" + + """ + hmtnote \\ + annotate \\ + $vcf \\ +- ${prefix}_annotated.vcf \\ ++ ${prefix}.vcf \\ + $args + + cat <<-END_VERSIONS > versions.yml +@@ -34,9 +34,9 @@ + END_VERSIONS + """ + stub: +- def prefix = task.ext.prefix ?: "${meta.id}" ++ prefix = task.ext.prefix ?: "${meta.id}" + """ +- touch ${prefix}_annotated.vcf ++ touch ${prefix}.vcf + cat <<-END_VERSIONS > versions.yml + "${task.process}": + hmtnote: \$(echo \$(hmtnote --version 2>&1) | sed 's/^.*hmtnote, version //; s/Using.*\$//' )) + +************************************************************ diff --git a/modules/nf-core/hmtnote/annotate/main.nf b/modules/nf-core/hmtnote/annotate/main.nf index 2fca73f4..8d727df3 100644 --- a/modules/nf-core/hmtnote/annotate/main.nf +++ b/modules/nf-core/hmtnote/annotate/main.nf @@ -11,7 +11,7 @@ process HMTNOTE_ANNOTATE { tuple val(meta), path(vcf) output: - tuple val(meta), path("*_annotated.vcf"), emit: vcf + tuple val(meta), path("${prefix}.vcf"), emit: vcf path "versions.yml" , emit: versions when: @@ -19,13 +19,13 @@ process HMTNOTE_ANNOTATE { script: def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" + prefix = task.ext.prefix ?: "${meta.id}" """ hmtnote \\ annotate \\ $vcf \\ - ${prefix}_annotated.vcf \\ + ${prefix}.vcf \\ $args cat <<-END_VERSIONS > versions.yml @@ -34,9 +34,9 @@ process HMTNOTE_ANNOTATE { END_VERSIONS """ stub: - def prefix = task.ext.prefix ?: "${meta.id}" + prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${prefix}_annotated.vcf + touch ${prefix}.vcf cat <<-END_VERSIONS > versions.yml "${task.process}": hmtnote: \$(echo \$(hmtnote --version 2>&1) | sed 's/^.*hmtnote, version //; s/Using.*\$//' )) diff --git a/modules/nf-core/svdb/merge/main.nf b/modules/nf-core/svdb/merge/main.nf index 0d9967dd..827d11b3 100644 --- a/modules/nf-core/svdb/merge/main.nf +++ b/modules/nf-core/svdb/merge/main.nf @@ -11,7 +11,7 @@ process SVDB_MERGE { val (priority) output: - tuple val(meta), path("*_sv_merge.vcf.gz"), emit: vcf + tuple val(meta), path("*.vcf.gz"), emit: vcf path "versions.yml" , emit: versions when: @@ -35,8 +35,8 @@ process SVDB_MERGE { $args \\ $prio \\ --vcf $input \\ - > ${prefix}_sv_merge.vcf - bgzip ${prefix}_sv_merge.vcf + > ${prefix}.vcf + bgzip ${prefix}.vcf cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -48,7 +48,7 @@ process SVDB_MERGE { stub: def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${prefix}_sv_merge.vcf.gz + touch ${prefix}.vcf.gz cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/svdb/merge/tests/main.nf.test b/modules/nf-core/svdb/merge/tests/main.nf.test new file mode 100644 index 00000000..f06b5998 --- /dev/null +++ b/modules/nf-core/svdb/merge/tests/main.nf.test @@ -0,0 +1,57 @@ +nextflow_process { + + name "Test Process SVDB_MERGE" + script "modules/nf-core/svdb/merge/main.nf" + process "SVDB_MERGE" + tag "modules" + tag "modules_nfcore" + tag "svdb" + tag "svdb/merge" + + test("test_svdb_merge") { + + when { + process { + """ + input[0] = [[ id:'test' ], // meta map + [file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test2_vcf'], checkIfExists: true) ] + ] + input[1] = [ 'tiddit', 'cnvnator'] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert path(process.out.vcf.get(0).get(1)).linesGzip.contains("##fileformat=VCFv4.1") } + ) + } + + } + + test("test_svdb_merge_noprio") { + + when { + process { + """ + input[0] = [[ id:'test' ], // meta map + [file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test2_vcf'], checkIfExists: true) ] + ] + input[1] = [] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert path(process.out.vcf.get(0).get(1)).linesGzip.contains("##fileformat=VCFv4.1") } + ) + } + + } + +} diff --git a/modules/nf-core/svdb/merge/tests/tags.yml b/modules/nf-core/svdb/merge/tests/tags.yml new file mode 100644 index 00000000..8501d907 --- /dev/null +++ b/modules/nf-core/svdb/merge/tests/tags.yml @@ -0,0 +1,2 @@ +svdb/merge: + - modules/nf-core/svdb/merge/** diff --git a/subworkflows/local/annotate_genome_snvs.nf b/subworkflows/local/annotate_genome_snvs.nf index fe59303d..e2f10a1e 100644 --- a/subworkflows/local/annotate_genome_snvs.nf +++ b/subworkflows/local/annotate_genome_snvs.nf @@ -93,7 +93,7 @@ workflow ANNOTATE_GENOME_SNVS { .combine(ch_split_intervals) .map { meta, vcf, tbi, interval -> - return [meta + [scatterid:interval.baseName], vcf, tbi, interval] + return [meta + [scatterid:interval.baseName, prefix: vcf.simpleName], vcf, tbi, interval] } .set { ch_vcf_scatter_in } @@ -118,7 +118,7 @@ workflow ANNOTATE_GENOME_SNVS { selvar: it[2].equals(null) return [it[0], it[1]] cadd: !(it[2].equals(null)) - return [it[0], it[2]] + return [it[0] + [prefix: it[0].prefix + "_cadd"], it[2]] } .set { ch_for_mix } @@ -156,9 +156,13 @@ workflow ANNOTATE_GENOME_SNVS { BCFTOOLS_CONCAT (ch_concat_in) - TABIX_BCFTOOLS_CONCAT (BCFTOOLS_CONCAT.out.vcf) + BCFTOOLS_CONCAT.out.vcf + .map { meta, vcf -> [meta - meta.subMap('prefix'), vcf] } + .set { ch_concat_out } - ch_vep_ann = BCFTOOLS_CONCAT.out.vcf + TABIX_BCFTOOLS_CONCAT (ch_concat_out) + + ch_vep_ann = ch_concat_out ch_vep_index = TABIX_BCFTOOLS_CONCAT.out.tbi ch_versions = ch_versions.mix(BCFTOOLS_ROH.out.versions) diff --git a/subworkflows/local/annotate_mt_snvs.nf b/subworkflows/local/annotate_mt_snvs.nf index e7b8ae6a..5cbf261f 100644 --- a/subworkflows/local/annotate_mt_snvs.nf +++ b/subworkflows/local/annotate_mt_snvs.nf @@ -46,9 +46,9 @@ workflow ANNOTATE_MT_SNVS { .join(ch_cadd_vcf, remainder: true) // If CADD is not run then the third element in this channel will be `null` .branch { it -> // If CADD is run, then "it" will be [[meta],selvar.vcf,cadd.vcf], else [[meta],selvar.vcf,null] merged: it[2].equals(null) - return [it[0], it[1]] + return [it[0]+ [prefix: it[1].simpleName + "_vep"], it[1]] cadd: !(it[2].equals(null)) - return [it[0], it[2]] + return [it[0] + [prefix: it[1].simpleName + "_cadd_vep"], it[2]] } .set { ch_for_mix } @@ -73,15 +73,18 @@ workflow ANNOTATE_MT_SNVS { TABIX_TABIX_MT(ENSEMBLVEP_MT.out.vcf) ENSEMBLVEP_MT.out.vcf .join(TABIX_TABIX_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) - .map { meta, vcf, tbi -> return [meta, vcf, tbi, []]} + .map { meta, vcf, tbi -> return [meta + [prefix: meta.prefix + "_vcfanno"], vcf, tbi, []]} .set { ch_in_vcfanno } - VCFANNO_MT(ch_in_vcfanno, ch_vcfanno_toml, [], ch_vcfanno_resources) + VCFANNO_MT(ch_in_vcfanno, ch_vcfanno_toml, [], ch_vcfanno_resources).vcf + .map { meta, vcf -> return [meta + [prefix: meta.prefix + "_hmtnote"], vcf]} + .set {ch_hmtnote_in} // HMTNOTE ANNOTATE - HMTNOTE_ANNOTATE(VCFANNO_MT.out.vcf) - HMTNOTE_ANNOTATE.out.vcf.map{meta, vcf -> - return [meta, WorkflowRaredisease.replaceSpacesInInfoColumn(vcf, vcf.parent.toString(), vcf.baseName)] + HMTNOTE_ANNOTATE(ch_hmtnote_in) + HMTNOTE_ANNOTATE.out.vcf + .map{meta, vcf -> + return [meta, WorkflowRaredisease.replaceSpacesInInfoColumn(vcf, vcf.parent.toString(), vcf.baseName)] } .set { ch_hmtnote_reformatted } ZIP_TABIX_HMTNOTE(ch_hmtnote_reformatted) diff --git a/subworkflows/local/annotate_structural_variants.nf b/subworkflows/local/annotate_structural_variants.nf index d2d42027..db73f5a1 100644 --- a/subworkflows/local/annotate_structural_variants.nf +++ b/subworkflows/local/annotate_structural_variants.nf @@ -95,7 +95,9 @@ workflow ANNOTATE_STRUCTURAL_VARIANTS { PICARD_SORTVCF(ch_querypedbed_out, ch_genome_fasta, ch_genome_dictionary) - PICARD_SORTVCF.out.vcf.map { meta, vcf -> return [meta,vcf,[]] }.set { ch_sortvcf } + PICARD_SORTVCF.out.vcf + .map { meta, vcf -> return [meta,vcf,[]] } + .set { ch_sortvcf } BCFTOOLS_VIEW(ch_sortvcf, [], [], []) .vcf From 2d3982cdbf1c1a282927c50979c9f9148825237d Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 16 Feb 2024 09:24:32 +0100 Subject: [PATCH 345/387] update mt_delsuffix --- modules/local/mt_deletion_script.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/mt_deletion_script.nf b/modules/local/mt_deletion_script.nf index 02d55876..f363b949 100644 --- a/modules/local/mt_deletion_script.nf +++ b/modules/local/mt_deletion_script.nf @@ -38,7 +38,7 @@ process MT_DELETION { stub: def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${prefix}_mt_del.txt + touch ${prefix}.txt cat <<-END_VERSIONS > versions.yml "${task.process}": From 62d5ae74022c14476cd13370992e3308b8df7eda Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 16 Feb 2024 09:25:16 +0100 Subject: [PATCH 346/387] update docs --- docs/output.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/output.md b/docs/output.md index 61ec49c8..e98721db 100644 --- a/docs/output.md +++ b/docs/output.md @@ -456,7 +456,7 @@ The pipeline for mitochondrial variant discovery, using Mutect2, uses a high sen [MT deletion script](https://github.com/dnil/mitosign/blob/master/run_mt_del_check.sh) lists the fraction of mitochondrially aligning read pairs (per 1000) that appear discordant, as defined by an insert size of more than 1.2 kb (and less than 15 kb due to the circular nature of the genome) using samtools. - `call_sv/mitochondria` - - `_mt_del.txt`: file containing deletions. + - `.txt`: file containing deletions. ##### eKLIPse From 3099751ff9f7d469e01361e45ad5d0e3c00c27bf Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 16 Feb 2024 13:55:29 +0100 Subject: [PATCH 347/387] review suggestions --- conf/modules/call_snv.config | 2 +- conf/modules/call_sv_MT.config | 1 + docs/output.md | 10 +++++----- 3 files changed, 7 insertions(+), 6 deletions(-) diff --git a/conf/modules/call_snv.config b/conf/modules/call_snv.config index 92eda8cf..1170d515 100644 --- a/conf/modules/call_snv.config +++ b/conf/modules/call_snv.config @@ -21,7 +21,7 @@ process { withName: '.*CALL_SNV:GATK4_SELECTVARIANTS' { ext.args = { "--exclude-intervals ${params.mito_name}" } - ext.prefix = { "${meta.id}_nomito" } + ext.prefix = { "${meta.id}_snv" } publishDir = [ path: { "${params.outdir}/call_snv/genome" }, mode: params.publish_dir_mode, diff --git a/conf/modules/call_sv_MT.config b/conf/modules/call_sv_MT.config index b7022829..05f98e84 100644 --- a/conf/modules/call_sv_MT.config +++ b/conf/modules/call_sv_MT.config @@ -19,6 +19,7 @@ process { withName: '.*CALL_SV_MT:MT_DELETION' { ext.args = '-s --insert-size 16000' + ext.prefix = { "${meta.id}_mitochondria_deletions" } publishDir = [ path: { "${params.outdir}/call_sv/mitochondria" }, mode: params.publish_dir_mode, diff --git a/docs/output.md b/docs/output.md index e98721db..281ac7dd 100644 --- a/docs/output.md +++ b/docs/output.md @@ -266,8 +266,8 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ Output files - `call_snv/genome` - - `_nomito.vcf.gz`: normalized vcf file containing no MT variants. - - `_nomito.vcf.gz.tbi`: index of the vcf file containing no MT variants. + - `_snv.vcf.gz`: normalized vcf file containing no MT variants. + - `_snv.vcf.gz.tbi`: index of the vcf file containing no MT variants. @@ -279,8 +279,8 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support. Output files - `call_snv/genome` - - `_nomito.vcf.gz`: normalized vcf file containing no MT variants. - - `_nomito.vcf.gz.tbi`: index of the vcf file containing no MT variants. + - `_snv.vcf.gz`: normalized vcf file containing no MT variants. + - `_snv.vcf.gz.tbi`: index of the vcf file containing no MT variants. @@ -456,7 +456,7 @@ The pipeline for mitochondrial variant discovery, using Mutect2, uses a high sen [MT deletion script](https://github.com/dnil/mitosign/blob/master/run_mt_del_check.sh) lists the fraction of mitochondrially aligning read pairs (per 1000) that appear discordant, as defined by an insert size of more than 1.2 kb (and less than 15 kb due to the circular nature of the genome) using samtools. - `call_sv/mitochondria` - - `.txt`: file containing deletions. + - `_mitochondria_deletions.txt`: file containing deletions. ##### eKLIPse From 39a1400231a4feb7bc562b3945b378cbcd5105fb Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 20 Feb 2024 15:30:05 +0000 Subject: [PATCH 348/387] Template update for nf-core/tools version 2.13 --- .editorconfig | 9 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/branch.yml | 2 +- .github/workflows/ci.yml | 7 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 17 +- .github/workflows/linting.yml | 12 +- .github/workflows/linting_comment.yml | 4 +- .github/workflows/release-announcements.yml | 11 +- README.md | 5 +- assets/multiqc_config.yml | 2 + assets/schema_input.json | 21 +- bin/check_samplesheet.py | 259 ----------- conf/modules.config | 8 - lib/NfcoreTemplate.groovy | 356 -------------- lib/Utils.groovy | 47 -- lib/WorkflowMain.groovy | 77 --- lib/WorkflowRaredisease.groovy | 122 ----- main.nf | 100 ++-- modules.json | 28 +- modules/local/samplesheet_check.nf | 31 -- .../dumpsoftwareversions/environment.yml | 7 - .../custom/dumpsoftwareversions/main.nf | 24 - .../custom/dumpsoftwareversions/meta.yml | 37 -- .../templates/dumpsoftwareversions.py | 102 ---- .../dumpsoftwareversions/tests/main.nf.test | 43 -- .../tests/main.nf.test.snap | 33 -- .../dumpsoftwareversions/tests/tags.yml | 2 - modules/nf-core/fastqc/tests/main.nf.test | 14 +- .../nf-core/fastqc/tests/main.nf.test.snap | 76 ++- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- modules/nf-core/multiqc/tests/main.nf.test | 13 +- .../nf-core/multiqc/tests/main.nf.test.snap | 32 +- nextflow.config | 5 +- nextflow_schema.json | 1 + pyproject.toml | 8 +- subworkflows/local/input_check.nf | 44 -- .../utils_nfcore_raredisease_pipeline/main.nf | 247 ++++++++++ .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 +++ .../tests/main.function.nf.test.snap | 12 + .../tests/main.workflow.nf.test | 123 +++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 440 ++++++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 ++++++ .../tests/main.function.nf.test.snap | 138 ++++++ .../tests/main.workflow.nf.test | 29 ++ .../tests/main.workflow.nf.test.snap | 15 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 +++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 ++++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + workflows/raredisease.nf | 133 ++---- 61 files changed, 2108 insertions(+), 1406 deletions(-) delete mode 100755 bin/check_samplesheet.py delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100644 lib/Utils.groovy delete mode 100755 lib/WorkflowMain.groovy delete mode 100755 lib/WorkflowRaredisease.groovy delete mode 100644 modules/local/samplesheet_check.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml delete mode 100644 subworkflows/local/input_check.nf create mode 100644 subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/.editorconfig b/.editorconfig index 9b990088..dd9ffa53 100644 --- a/.editorconfig +++ b/.editorconfig @@ -18,7 +18,12 @@ end_of_line = unset insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset -indent_size = unset +[/subworkflows/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset [/assets/email*] indent_size = unset @@ -28,5 +33,5 @@ indent_size = unset indent_style = unset # ignore python -[*.{py}] +[*.{py,md}] indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 2919151f..33080301 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 4ed98bab..8c3f5cb0 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 54998898..ede6597b 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v2 + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 612fc2fb..e84295b2 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,13 +28,16 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 with: version: "${{ matrix.NXF_VER }}" + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: Run pipeline with test data # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index e37cfda5..0b6b1f27 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v9 + - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458a..f823210d 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -6,6 +6,11 @@ name: Test successful pipeline download with 'nf-core download' # - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. on: workflow_dispatch: + inputs: + testbranch: + description: "The specific branch you wish to utilize for the test execution of nf-core download." + required: true + default: "dev" pull_request: types: - opened @@ -23,13 +28,13 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" - - uses: eWaterCycle/setup-singularity@v7 + - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: singularity-version: 3.8.3 @@ -42,13 +47,13 @@ jobs: run: | echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} - echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} - name: Download the pipeline env: NXF_SINGULARITY_CACHEDIR: ./ run: | - nf-core download ${{ env.REPO_LOWERCASE }} \ + nf-core download ${{ env.REPO_LOWERCASE }} \ --revision ${{ env.REPO_BRANCH }} \ --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ --compress "none" \ @@ -64,4 +69,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 81cd098e..748b4311 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,10 +14,10 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Set up Python 3.11 - uses: actions/setup-python@v5 + uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: 3.11 cache: "pip" @@ -32,12 +32,12 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v4 + uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 147bcd10..b706875f 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v3 + uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 with: workflow: linting.yml workflow_conclusion: completed @@ -21,7 +21,7 @@ jobs: run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@v2 + uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 21ac3f06..c3674af2 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -9,6 +9,11 @@ jobs: toot: runs-on: ubuntu-latest steps: + - name: get topics and convert to hashtags + id: get_topics + run: | + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + - uses: rzr/fediverse-action@master with: access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} @@ -20,11 +25,13 @@ jobs: Please see the changelog: ${{ github.event.release.html_url }} + ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics + send-tweet: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +63,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.1.0 + - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/README.md b/README.md index 1d2a44f5..2afc405b 100644 --- a/README.md +++ b/README.md @@ -4,8 +4,9 @@ nf-core/raredisease -[![GitHub Actions CI Status](https://github.com/nf-core/raredisease/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/raredisease/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/nf-core/raredisease/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/raredisease/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/raredisease/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) + +[![GitHub Actions CI Status](https://github.com/nf-core/raredisease/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/raredisease/actions/workflows/ci.yml) +[![GitHub Actions Linting Status](https://github.com/nf-core/raredisease/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/raredisease/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/raredisease/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 14de2020..8c639b61 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -11,3 +11,5 @@ report_section_order: order: -1002 export_plots: true + +disable_version_detection: true diff --git a/assets/schema_input.json b/assets/schema_input.json index 4ba2de4c..be651264 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,25 +10,22 @@ "sample": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] }, "fastq_1": { "type": "string", + "format": "file-path", + "exists": true, "pattern": "^\\S+\\.f(ast)?q\\.gz$", "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" }, "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" } }, "required": ["sample", "fastq_1"] diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index 4a758fe0..00000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,259 +0,0 @@ -#!/usr/bin/env python - - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging -import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ) - - def __init__( - self, - sample_col="sample", - first_col="fastq_1", - second_col="fastq_2", - single_col="single_end", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - first_col (str): The name of the column that contains the first (or only) - FASTQ file path (default "fastq_1"). - second_col (str): The name of the column that contains the second (if any) - FASTQ file path (default "fastq_2"). - single_col (str): The name of the new column that will be inserted and - records whether the sample contains single- or paired-end sequencing - reads (default "single_end"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._first_col = first_col - self._second_col = second_col - self._single_col = single_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_first(row) - self._validate_second(row) - self._validate_pair(row) - self._seen.add((row[self._sample_col], row[self._first_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_first(self, row): - """Assert that the first FASTQ entry is non-empty and has the right format.""" - if len(row[self._first_col]) <= 0: - raise AssertionError("At least the first FASTQ file is required.") - self._validate_fastq_format(row[self._first_col]) - - def _validate_second(self, row): - """Assert that the second FASTQ entry has the right format if it exists.""" - if len(row[self._second_col]) > 0: - self._validate_fastq_format(row[self._second_col]) - - def _validate_pair(self, row): - """Assert that read pairs have the same file extension. Report pair status.""" - if row[self._first_col] and row[self._second_col]: - row[self._single_col] = False - first_col_suffix = Path(row[self._first_col]).suffixes[-2:] - second_col_suffix = Path(row[self._second_col]).suffixes[-2:] - if first_col_suffix != second_col_suffix: - raise AssertionError("FASTQ pairs must have the same file extensions.") - else: - row[self._single_col] = True - - def _validate_fastq_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The FASTQ file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. - - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" - - -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) - - -def sniff_format(handle): - """ - Detect the tabular format. - - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - dialect = sniffer.sniff(peek) - return dialect - - -def check_samplesheet(file_in, file_out): - """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. Also add - an additional column which records whether one or two FASTQ reads were found. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - - """ - required_columns = {"sample", "fastq_1", "fastq_2"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") - sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - header.insert(1, "single_end") - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) - - -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - return parser.parse_args(argv) - - -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/conf/modules.config b/conf/modules.config index d91c6aba..e3ea8fa6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,14 +18,6 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: SAMPLESHEET_CHECK { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: FASTQC { ext.args = '--quiet' } diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index e248e4c3..00000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,356 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") - sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); - output_hf.delete() - - // Write summary e-mail TXT to a file - def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); - output_tf.delete() - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Dump pipeline parameters in a json file - // - public static void dump_parameters(workflow, params) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) - - FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") - temp_pf.delete() - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100644 index 8d030f4e..00000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index 96098ebf..00000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,77 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the nf-core/raredisease pipeline -// - -import nextflow.Nextflow - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log, args) { - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - // Check that the profile doesn't contain spaces and doesn't end with a trailing comma - checkProfile(workflow.profile, args, log) - - // Check that conda channels are set-up correctly - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") - } - } - // - // Get attribute from genome config file e.g. fasta - // - public static Object getGenomeAttribute(params, attribute) { - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - return params.genomes[ params.genome ][ attribute ] - } - } - return null - } - - // - // Exit pipeline if --profile contains spaces - // - private static void checkProfile(profile, args, log) { - if (profile.endsWith(',')) { - Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - if (args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - } -} diff --git a/lib/WorkflowRaredisease.groovy b/lib/WorkflowRaredisease.groovy deleted file mode 100755 index 35204d84..00000000 --- a/lib/WorkflowRaredisease.groovy +++ /dev/null @@ -1,122 +0,0 @@ -// -// This file holds several functions specific to the workflow/raredisease.nf in the nf-core/raredisease pipeline -// - -import nextflow.Nextflow -import groovy.text.SimpleTemplateEngine - -class WorkflowRaredisease { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - - genomeExistsError(params, log) - - - if (!params.fasta) { - Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - } - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

$group

\n" - summary_section += " \n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Generate methods description for MultiQC - // - - public static String toolCitationText(params) { - - // TODO nf-core: Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text - } - - public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text - } - - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file - def meta = [:] - meta.workflow = run_workflow.toMap() - meta["manifest_map"] = run_workflow.manifest.toMap() - - // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " - - // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) - - - def methods_text = mqc_methods_yaml.text - - def engine = new SimpleTemplateEngine() - def description_html = engine.createTemplate(methods_text).make(meta) - - return description_html - } - - // - // Exit pipeline if incorrect --genome key provided - // - private static void genomeExistsError(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - Nextflow.error(error_string) - } - } -} diff --git a/main.nf b/main.nf index bce661f7..d7987755 100644 --- a/main.nf +++ b/main.nf @@ -13,66 +13,96 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// TODO nf-core: Remove this line if you don't need a FASTA file -// This is an example of how to use getGenomeAttribute() to fetch parameters -// from igenomes.config using `--genome` -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +include { RAREDISEASE } from './workflows/raredisease' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_raredisease_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_raredisease_pipeline' + +include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_raredisease_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE & PRINT PARAMETER SUMMARY + GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { validateParameters; paramsHelp } from 'plugin/nf-validation' - -// Print help message if needed -if (params.help) { - def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) - def citation = '\n' + WorkflowMain.citation(workflow) + '\n' - def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" - log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) - System.exit(0) -} - -// Validate input parameters -if (params.validate_params) { - validateParameters() -} - -WorkflowMain.initialise(workflow, params, log, args) +// TODO nf-core: Remove this line if you don't need a FASTA file +// This is an example of how to use getGenomeAttribute() to fetch parameters +// from igenomes.config using `--genome` +params.fasta = getGenomeAttribute('fasta') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - NAMED WORKFLOW FOR PIPELINE + NAMED WORKFLOWS FOR PIPELINE ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { RAREDISEASE } from './workflows/raredisease' - // -// WORKFLOW: Run main nf-core/raredisease analysis pipeline +// WORKFLOW: Run main analysis pipeline depending on type of input // workflow NFCORE_RAREDISEASE { - RAREDISEASE () -} + take: + samplesheet // channel: samplesheet read in from --input + + main: + + // + // WORKFLOW: Run pipeline + // + RAREDISEASE ( + samplesheet + ) + + emit: + multiqc_report = RAREDISEASE.out.multiqc_report // channel: /path/to/multiqc_report.html + +} /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN ALL WORKFLOWS + RUN MAIN WORKFLOW ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// WORKFLOW: Execute a single named workflow for the pipeline -// See: https://github.com/nf-core/rnaseq/issues/619 -// workflow { - NFCORE_RAREDISEASE () + + main: + + // + // SUBWORKFLOW: Run initialisation tasks + // + PIPELINE_INITIALISATION ( + params.version, + params.help, + params.validate_params, + params.monochrome_logs, + args, + params.outdir, + params.input + ) + + // + // WORKFLOW: Run main workflow + // + NFCORE_RAREDISEASE ( + PIPELINE_INITIALISATION.out.samplesheet + ) + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + NFCORE_RAREDISEASE.out.multiqc_report + ) } /* diff --git a/modules.json b/modules.json index ab7892e3..06b7b0d7 100644 --- a/modules.json +++ b/modules.json @@ -5,22 +5,36 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, "fastqc": { "branch": "master", - "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", + "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", "installed_by": ["modules"] } } + }, + "subworkflows": { + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + } + } } } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index 4dc269ea..00000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -process SAMPLESHEET_CHECK { - tag "$samplesheet" - label 'process_single' - - conda "conda-forge::python=3.8.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" - - input: - path samplesheet - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // This script is bundled with the pipeline, in nf-core/raredisease/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml deleted file mode 100644 index 9b3272bc..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: custom_dumpsoftwareversions -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf deleted file mode 100644 index f2187611..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ /dev/null @@ -1,24 +0,0 @@ -process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_single' - - // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" - - input: - path versions - - output: - path "software_versions.yml" , emit: yml - path "software_versions_mqc.yml", emit: mqc_yml - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - template 'dumpsoftwareversions.py' -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml deleted file mode 100644 index 5f15a5fd..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ /dev/null @@ -1,37 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: custom_dumpsoftwareversions -description: Custom module used to dump software versions within the nf-core pipeline template -keywords: - - custom - - dump - - version -tools: - - custom: - description: Custom module used to dump software versions within the nf-core pipeline template - homepage: https://github.com/nf-core/tools - documentation: https://github.com/nf-core/tools - licence: ["MIT"] -input: - - versions: - type: file - description: YML file containing software versions - pattern: "*.yml" -output: - - yml: - type: file - description: Standard YML file containing software versions - pattern: "software_versions.yml" - - mqc_yml: - type: file - description: MultiQC custom content YML file containing software versions - pattern: "software_versions_mqc.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" -maintainers: - - "@drpatelh" - - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py deleted file mode 100755 index e55b8d43..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ /dev/null @@ -1,102 +0,0 @@ -#!/usr/bin/env python - - -"""Provide functions to merge multiple versions.yml files.""" - - -import platform -from textwrap import dedent - -import yaml - - -def _make_versions_html(versions): - """Generate a tabular HTML output of all versions for MultiQC.""" - html = [ - dedent( - """\\ - -
    \n" - for (param in group_params.keySet()) { - summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" - } - summary_section += "
    Process Name \\", + " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    File typeConventional base calls
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    - - - - - - - - """ - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f"""\\ - - - - - - """ - ) - ) - html.append("") - html.append("
    Process Name Software Version
    {process if (i == 0) else ''}{tool}{version}
    ") - return "\\n".join(html) - - -def main(): - """Load all version files and generate merged output.""" - versions_this_module = {} - versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, - } - - with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - - # aggregate versions by the module name (derived from fully-qualified process name) - versions_by_module = {} - for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - - versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", - } - - versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), - } - - with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) - with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - - with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) - - -if __name__ == "__main__": - main() diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test deleted file mode 100644 index b1e1630b..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ /dev/null @@ -1,43 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" - script "../main.nf" - process "CUSTOM_DUMPSOFTWAREVERSIONS" - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "dumpsoftwareversions" - tag "custom/dumpsoftwareversions" - - test("Should run without failures") { - when { - process { - """ - def tool1_version = ''' - TOOL1: - tool1: 0.11.9 - '''.stripIndent() - - def tool2_version = ''' - TOOL2: - tool2: 1.9 - '''.stripIndent() - - input[0] = Channel.of(tool1_version, tool2_version).collectFile() - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.versions, - file(process.out.mqc_yml[0]).readLines()[0..10], - file(process.out.yml[0]).readLines()[0..7] - ).match() - } - ) - } - } -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap deleted file mode 100644 index 5f59a936..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ /dev/null @@ -1,33 +0,0 @@ -{ - "Should run without failures": { - "content": [ - [ - "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" - ], - [ - "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", - " \\n\\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n \\n \\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n\\n\\n \\n\\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\" - ], - [ - "CUSTOM_DUMPSOFTWAREVERSIONS:", - " python: 3.11.7", - " yaml: 5.4.1", - "TOOL1:", - " tool1: 0.11.9", - "TOOL2:", - " tool2: '1.9'", - "Workflow:" - ] - ], - "timestamp": "2024-01-09T23:01:18.710682" - } -} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml deleted file mode 100644 index 405aa24a..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/dumpsoftwareversions: - - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 1f21c664..70edae4d 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -33,7 +33,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_single") } ) } } @@ -63,7 +63,7 @@ nextflow_process { { assert path(process.out.html[0][1][0]).text.contains("") }, { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } ) } } @@ -89,7 +89,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } ) } } @@ -115,7 +115,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } ) } } @@ -153,7 +153,7 @@ nextflow_process { { assert path(process.out.html[0][1][2]).text.contains("") }, { assert path(process.out.html[0][1][3]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } ) } } @@ -179,7 +179,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } ) } } @@ -204,7 +204,7 @@ nextflow_process { { assert process.success }, { assert snapshot(process.out.html.collect { file(it[1]).getName() } + process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match() } + process.out.versions ).match("fastqc_stub") } ) } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 5d624bb8..86f7c311 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,5 +1,17 @@ { - "sarscov2 single-end [fastq] - stub": { + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { "content": [ [ "test.html", @@ -7,14 +19,70 @@ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:40:57.254299" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" }, - "versions": { + "fastqc_versions_custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:36:50.033627" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 7625b752..2212096a 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.19 + - bioconda::multiqc=1.20 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1b9f7c43..354f4430 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : + 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index d0438eda..f1c4242e 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -3,6 +3,7 @@ nextflow_process { name "Test Process MULTIQC" script "../main.nf" process "MULTIQC" + tag "modules" tag "modules_nfcore" tag "multiqc" @@ -12,7 +13,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -25,7 +26,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_single") } ) } @@ -36,7 +37,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] @@ -49,7 +50,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_config") } ) } } @@ -61,7 +62,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -75,7 +76,7 @@ nextflow_process { { assert snapshot(process.out.report.collect { file(it).getName() } + process.out.data.collect { file(it).getName() } + process.out.plots.collect { file(it).getName() } + - process.out.versions ).match() } + process.out.versions ).match("multiqc_stub") } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index d37e7304..c204b488 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -1,21 +1,41 @@ { - "versions": { + "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:02:49.911994" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:28:51.744211298" }, - "sarscov2 single-end [fastqc] - stub": { + "multiqc_stub": { "content": [ [ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:03:14.524346" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:28.847433492" + }, + "multiqc_versions_config": { + "content": [ + [ + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:13.223621555" } } \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 2d608ff7..5469bb1b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,9 +16,7 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - - - // MultiQC options + fasta = null// MultiQC options multiqc_config = null multiqc_title = null multiqc_logo = null @@ -43,7 +41,6 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - // Max resource options // Defaults only, expecting to be overwritten diff --git a/nextflow_schema.json b/nextflow_schema.json index 923259ef..2077e3bd 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -16,6 +16,7 @@ "type": "string", "format": "file-path", "exists": true, + "schema": "assets/schema_input.json", "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", "description": "Path to comma-separated file containing information about the samples in the experiment.", diff --git a/pyproject.toml b/pyproject.toml index 7d08e1c8..56110621 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -3,11 +3,13 @@ [tool.ruff] line-length = 120 target-version = "py38" -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] cache-dir = "~/.cache/ruff" -[tool.ruff.isort] +[tool.ruff.lint] +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] + +[tool.ruff.lint.isort] known-first-party = ["nf_core"] -[tool.ruff.per-file-ignores] +[tool.ruff.lint.per-file-ignores] "__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf deleted file mode 100644 index 0aecf87f..00000000 --- a/subworkflows/local/input_check.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// Check input samplesheet and get read channels -// - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta -} diff --git a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf new file mode 100644 index 00000000..dc90ad90 --- /dev/null +++ b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf @@ -0,0 +1,247 @@ +// +// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { fromSamplesheet } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir // string: The output directory where the results will be saved + input // string: Path to input samplesheet + + main: + + ch_versions = Channel.empty() + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = nfCoreLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + // + // Custom validation for pipeline parameters + // + validateInputParameters() + + // + // Create channel from input file provided through params.input + // + Channel + .fromSamplesheet("input") + .map { + meta, fastq_1, fastq_2 -> + if (!fastq_2) { + return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] + } else { + return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] + } + } + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .map { + meta, fastqs -> + return [ meta, fastqs.flatten() ] + } + .set { ch_samplesheet } + + emit: + samplesheet = ch_samplesheet + versions = ch_versions +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ +// +// Check and validate pipeline parameters +// +def validateInputParameters() { + genomeExistsError() +}// +// Validate channels from input samplesheet +// +def validateInputSamplesheet(input) { + def (metas, fastqs) = input[1..2] + + // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + if (!endedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") + } + + return [ metas[0], fastqs ] +} +// +// Get attribute from genome config file e.g. fasta +// +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} + +// +// Exit pipeline if incorrect --genome key provided +// +def genomeExistsError() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } +}// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + + + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 00000000..ac31f28f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 00000000..e5c3a0a8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..8ed4310c --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..db2030f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,12 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "timestamp": "2024-01-19T11:32:36.031083" + }, + "Test Function checkCondaChannels": { + "content": null, + "timestamp": "2024-01-19T11:32:50.456" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..f7c54bc6 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,123 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = params.outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = null + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 00000000..53574ffe --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 00000000..f8476112 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 00000000..a8b55d6f --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,440 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " ${workflow.manifest.doi}\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 00000000..d08d2434 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..1dc317f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..10f948e6 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,138 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "timestamp": "2024-02-09T15:43:55.145717" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "timestamp": "2024-01-19T11:34:13.548431224" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "timestamp": "2024-01-19T11:34:38.840454873" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "timestamp": "2024-01-19T11:34:22.24352016" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "timestamp": "2024-01-19T11:35:04.418416984" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "timestamp": "2024-01-19T11:34:55.420000755" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "timestamp": "2024-01-19T11:35:13.436366565" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..8940d32d --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 00000000..d07ce54c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,15 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "timestamp": "2024-01-19T11:35:22.538940073" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 00000000..d0a926bf --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 00000000..ac8523c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 00000000..2585b65d --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 00000000..3d4a6b04 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 00000000..517ee54e --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 00000000..7626c1c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 00000000..60b1cfff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index ac152bea..c9dd37ed 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -1,54 +1,15 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - PRINT PARAMS SUMMARY + IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -WorkflowRaredisease.initialise(params, log) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT LOCAL MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT NF-CORE MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// MODULE: Installed directly from nf-core/modules -// -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_raredisease_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -56,50 +17,45 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// Info required for completion email and summary -def multiqc_report = [] - workflow RAREDISEASE { - ch_versions = Channel.empty() + take: + ch_samplesheet // channel: samplesheet read in from --input - // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files - // - INPUT_CHECK ( - file(params.input) - ) - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema + main: + + ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() // // MODULE: Run FastQC // FASTQC ( - INPUT_CHECK.out.reads + ch_samplesheet ) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') - ) + // + // Collate and save software versions + // + softwareVersionsToYAML(ch_versions) + .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) + .set { ch_collated_versions } // // MODULE: MultiQC // - workflow_summary = WorkflowRaredisease.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowRaredisease.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) MULTIQC ( ch_multiqc_files.collect(), @@ -107,31 +63,10 @@ workflow RAREDISEASE { ch_multiqc_custom_config.toList(), ch_multiqc_logo.toList() ) - multiqc_report = MULTIQC.out.report.toList() -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.dump_parameters(workflow, params) - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) - } -} -workflow.onError { - if (workflow.errorReport.contains("Process requirement exceeds available memory")) { - println("🛑 Default resources exceed availability 🛑 ") - println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") - } + emit: + multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [ path(versions.yml) ] } /* From 0e10aec89ee2f9750993f3c0abb7194766847dd3 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 21 Feb 2024 00:30:28 +0100 Subject: [PATCH 349/387] update modules.json --- modules.json | 5 ----- 1 file changed, 5 deletions(-) diff --git a/modules.json b/modules.json index 3e3e0ebc..5c9cf3ff 100644 --- a/modules.json +++ b/modules.json @@ -101,11 +101,6 @@ "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, "deepvariant": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", From b8b151d37b498be0c342c25522205da84301ad87 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 21 Feb 2024 13:43:18 +0100 Subject: [PATCH 350/387] move function to utils --- subworkflows/local/annotate_mt_snvs.nf | 17 +---------------- .../utils_nfcore_raredisease_pipeline/main.nf | 18 ++++++++++++++++++ 2 files changed, 19 insertions(+), 16 deletions(-) diff --git a/subworkflows/local/annotate_mt_snvs.nf b/subworkflows/local/annotate_mt_snvs.nf index 7b6f6123..f28744f6 100644 --- a/subworkflows/local/annotate_mt_snvs.nf +++ b/subworkflows/local/annotate_mt_snvs.nf @@ -2,6 +2,7 @@ // Annotate MT // +include { replaceSpacesInInfoColumn } from './utils_nfcore_raredisease_pipeline/main' include { TABIX_TABIX as TABIX_TABIX_MT } from '../../modules/nf-core/tabix/tabix/main' include { ENSEMBLVEP_VEP as ENSEMBLVEP_MT } from '../../modules/nf-core/ensemblvep/vep/main' include { HAPLOGREP2_CLASSIFY as HAPLOGREP2_CLASSIFY_MT } from '../../modules/nf-core/haplogrep2/classify/main' @@ -109,19 +110,3 @@ workflow ANNOTATE_MT_SNVS { report = ENSEMBLVEP_MT.out.report // channel: [ path(html) ] versions = ch_versions // channel: [ path(versions.yml) ] } - -def replaceSpacesInInfoColumn(vcf_file, parent_dir, base_name) { - def outfile = new File(parent_dir + '/' + base_name + '_formatted.vcf') - def writer = outfile.newWriter() - vcf_file.eachLine { line -> - if (line.startsWith("#")) { - writer << line + "\n" - } else { - def split_str = line.tokenize("\t") - split_str[7] = split_str.getAt(7).replaceAll(" ","_") - writer << split_str.join("\t") + "\n" - } - } - writer.close() - return outfile -} diff --git a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf index 9af82548..a99c6ed0 100644 --- a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf @@ -187,6 +187,24 @@ def getGenomeAttribute(attribute) { } return null } +// +// Replace spaces in vcf files with underscores +// +def replaceSpacesInInfoColumn(vcf_file, parent_dir, base_name) { + def outfile = new File(parent_dir + '/' + base_name + '_formatted.vcf') + def writer = outfile.newWriter() + vcf_file.eachLine { line -> + if (line.startsWith("#")) { + writer << line + "\n" + } else { + def split_str = line.tokenize("\t") + split_str[7] = split_str.getAt(7).replaceAll(" ","_") + writer << split_str.join("\t") + "\n" + } + } + writer.close() + return outfile +} // // Exit pipeline if incorrect --genome key provided From 72b6fc43a5f2132918b3c9d5369afdc725625a70 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 21 Feb 2024 15:10:12 +0100 Subject: [PATCH 351/387] update ci --- .github/workflows/ci.yml | 7 ------- 1 file changed, 7 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index a50230b8..99541374 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -30,13 +30,6 @@ jobs: - "-profile test,docker" - "-profile test_one_sample,docker" steps: - - name: Free some space - run: | - sudo rm -rf /usr/share/dotnet - sudo rm -rf /opt/ghc - sudo rm -rf "/usr/local/share/boost" - sudo rm -rf "$AGENT_TOOLSDIRECTORY" - - name: Check out pipeline code uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 From 9a751d6c02aab7164f1ee748db5bbc8aa07c1cec Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 28 Feb 2024 13:39:58 +0100 Subject: [PATCH 352/387] vcf2cytosure rename samples --- assets/sample_id_map.json | 24 +++++++++++++++ conf/modules/generate_cytosure_files.config | 7 +++++ nextflow.config | 1 + nextflow_schema.json | 11 +++++++ subworkflows/local/generate_cytosure_files.nf | 29 +++++++++++++------ workflows/raredisease.nf | 3 ++ 6 files changed, 66 insertions(+), 9 deletions(-) create mode 100644 assets/sample_id_map.json diff --git a/assets/sample_id_map.json b/assets/sample_id_map.json new file mode 100644 index 00000000..aaba0319 --- /dev/null +++ b/assets/sample_id_map.json @@ -0,0 +1,24 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/raredisease/master/assets/mobile_element_references_schema.json", + "title": "Schema for customerid_internalid_map", + "description": "Schema for the file provided with params.customerid_internalid_map", + "type": "array", + "items": { + "type": "object", + "properties": { + "customer_id": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Customer id cannot contain spaces" + }, + "internal_id": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Internal id cannot contain spaces", + "meta": ["id"] + } + }, + "required": ["customer_id", "internal_id"] + } +} diff --git a/conf/modules/generate_cytosure_files.config b/conf/modules/generate_cytosure_files.config index 323b64e3..b052206c 100644 --- a/conf/modules/generate_cytosure_files.config +++ b/conf/modules/generate_cytosure_files.config @@ -33,6 +33,13 @@ process { ext.prefix = { ${meta.id} } } + withName: '.*GENERATE_CYTOSURE_FILES:BCFTOOLS_REHEADER_SV_VCF' { + beforeScript = { "echo ${meta.custid} > ${meta.custid}.txt" } + ext.args = { "--samples ${meta.custid}.txt" } + ext.args2 = "--output-type v" + ext.prefix = { ${meta.id} } + } + withName: '.*GENERATE_CYTOSURE_FILES:VCF2CYTOSURE' { ext.args = { [ meta.sex.equals(1) ? '--sex male' : '--sex female', diff --git a/nextflow.config b/nextflow.config index 110fd186..d8591f15 100644 --- a/nextflow.config +++ b/nextflow.config @@ -74,6 +74,7 @@ params { reduced_penetrance = null readcount_intervals = null rtg_truthvcfs = null + sample_id_map = null sequence_dictionary = null score_config_mt = null score_config_snv = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 8230597d..0fbfdad4 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -375,6 +375,17 @@ "help_text": "Should be Stranger's extended JSON as described at https://github.com/Clinical-Genomics/stranger/blob/master/stranger/resources/variant_catalog_grch37.json. This file is used by both ExpansionHunter and Stranger", "fa_icon": "fas fa-file" }, + "sample_id_map": { + "type": "string", + "exists": true, + "format": "file-path", + "description": "Path to a file containing internal ids and customer ids in csv format.", + "fa_icon": "fas fa-file-csv", + "mimetype": "text/csv", + "schema": "assets/sample_id_map.json", + "help_text": "Optional file to rename sample ids in the vcf2cytosure vcf", + "pattern": "^\\S+\\.csv$" + }, "vcf2cytosure_blacklist": { "type": "string", "help_text": "Optional file to blacklist regions for VCF2cytosure", diff --git a/subworkflows/local/generate_cytosure_files.nf b/subworkflows/local/generate_cytosure_files.nf index 561411ab..036ed595 100644 --- a/subworkflows/local/generate_cytosure_files.nf +++ b/subworkflows/local/generate_cytosure_files.nf @@ -2,16 +2,18 @@ // Convert VCF with structural variations to the “.CGH” format used by the CytoSure Interpret Software // -include { BCFTOOLS_VIEW as SPLIT_AND_FILTER_SV_VCF } from '../../modules/nf-core/bcftools/view/main' -include { TIDDIT_COV as TIDDIT_COV_VCF2CYTOSURE } from '../../modules/nf-core/tiddit/cov/main' -include { VCF2CYTOSURE } from '../../modules/nf-core/vcf2cytosure/main' +include { BCFTOOLS_VIEW as SPLIT_AND_FILTER_SV_VCF } from '../../modules/nf-core/bcftools/view/main' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_SV_VCF } from '../../modules/nf-core/bcftools/reheader/main' +include { TIDDIT_COV as TIDDIT_COV_VCF2CYTOSURE } from '../../modules/nf-core/tiddit/cov/main' +include { VCF2CYTOSURE } from '../../modules/nf-core/vcf2cytosure/main' workflow GENERATE_CYTOSURE_FILES { take: - ch_vcf // channel: [mandatory] [ val(meta), path(vcf) ] - ch_tbi // channel: [mandatory] [ val(meta), path(vcf_index) ] - ch_bam // channel: [mandatory] [ val(meta), path(bam) ] - ch_blacklist // channel: [optional] [path(blacklist)] + ch_vcf // channel: [mandatory] [ val(meta), path(vcf) ] + ch_tbi // channel: [mandatory] [ val(meta), path(vcf_index) ] + ch_bam // channel: [mandatory] [ val(meta), path(bam) ] + ch_sample_id_map // channel: [optional] [val(id), val(id)] + ch_blacklist // channel: [optional] [path(blacklist)] main: ch_versions = Channel.empty() @@ -24,14 +26,22 @@ workflow GENERATE_CYTOSURE_FILES { ch_bam.combine(ch_vcf_tbi).map { meta_sample, bam, meta_case, vcf, tbi -> - return [ meta_sample, vcf, tbi ] + new_meta = ['id':meta_sample.sample] + return [ new_meta, vcf, tbi ] }.set { ch_sample_vcf } // Split vcf into sample vcf:s and frequency filter SPLIT_AND_FILTER_SV_VCF ( ch_sample_vcf, [], [], [] ) + SPLIT_AND_FILTER_SV_VCF.out.vcf + .join(ch_sample_id_map) + .map { meta, vcf, custid -> return [meta+[custid:custid], vcf, [], []]} + .set { ch_reheader_in } + + BCFTOOLS_REHEADER_SV_VCF ( ch_reheader_in, [[:],[]] ) + VCF2CYTOSURE ( - SPLIT_AND_FILTER_SV_VCF.out.vcf, + BCFTOOLS_REHEADER_SV_VCF.out.vcf, TIDDIT_COV_VCF2CYTOSURE.out.cov, [[:], []], [[:], []], ch_blacklist @@ -40,6 +50,7 @@ workflow GENERATE_CYTOSURE_FILES { ch_versions = ch_versions.mix(TIDDIT_COV_VCF2CYTOSURE.out.versions.first()) ch_versions = ch_versions.mix(SPLIT_AND_FILTER_SV_VCF.out.versions.first()) ch_versions = ch_versions.mix(VCF2CYTOSURE.out.versions.first()) + ch_versions = ch_versions.mix(BCFTOOLS_REHEADER_SV_VCF.out.versions.first()) emit: versions = ch_versions // channel: [ versions.yml ] diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 1a5b85e0..ae4eb986 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -244,6 +244,8 @@ workflow RAREDISEASE { : Channel.value([]) ch_rtg_truthvcfs = params.rtg_truthvcfs ? Channel.fromPath(params.rtg_truthvcfs).collect() : Channel.value([]) + ch_sample_id_map = params.sample_id_map ? Channel.fromSamplesheet("sample_id_map") + : Channel.value([]) ch_score_config_mt = params.score_config_mt ? Channel.fromPath(params.score_config_mt).collect() : Channel.value([]) ch_score_config_snv = params.score_config_snv ? Channel.fromPath(params.score_config_snv).collect() @@ -613,6 +615,7 @@ workflow RAREDISEASE { ch_sv_annotate.vcf_ann, ch_sv_annotate.tbi, ch_mapped.genome_marked_bam, + ch_sample_id_map, ch_vcf2cytosure_blacklist ) ch_versions = ch_versions.mix(GENERATE_CYTOSURE_FILES.out.versions) From 1d3b525c79965cd7e34b9b70197e7ab92857c020 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 28 Feb 2024 20:32:15 +0100 Subject: [PATCH 353/387] update config --- conf/modules/generate_cytosure_files.config | 6 +- subworkflows/local/generate_cytosure_files.nf | 56 +++++++++++++++---- workflows/raredisease.nf | 2 +- 3 files changed, 48 insertions(+), 16 deletions(-) diff --git a/conf/modules/generate_cytosure_files.config b/conf/modules/generate_cytosure_files.config index b052206c..1cd076f3 100644 --- a/conf/modules/generate_cytosure_files.config +++ b/conf/modules/generate_cytosure_files.config @@ -30,14 +30,14 @@ process { '--output-type z', "--exclude 'gnomad_svAF > 0.05'" ].join(' ') } - ext.prefix = { ${meta.id} } + ext.prefix = { "${meta.id}" } } withName: '.*GENERATE_CYTOSURE_FILES:BCFTOOLS_REHEADER_SV_VCF' { beforeScript = { "echo ${meta.custid} > ${meta.custid}.txt" } ext.args = { "--samples ${meta.custid}.txt" } ext.args2 = "--output-type v" - ext.prefix = { ${meta.id} } + ext.prefix = { "${meta.custid}" } } withName: '.*GENERATE_CYTOSURE_FILES:VCF2CYTOSURE' { @@ -46,7 +46,7 @@ process { '--size 5000', '--maxbnd 5000' ].join(' ') } - ext.prefix = { "${meta.id}" } + ext.prefix = { "${meta.custid}" ? "${meta.custid}" : "${meta.id}" } publishDir = [ path: { "${params.outdir}/vcf2cytosure" }, mode: params.publish_dir_mode, diff --git a/subworkflows/local/generate_cytosure_files.nf b/subworkflows/local/generate_cytosure_files.nf index 036ed595..0f480a02 100644 --- a/subworkflows/local/generate_cytosure_files.nf +++ b/subworkflows/local/generate_cytosure_files.nf @@ -16,7 +16,8 @@ workflow GENERATE_CYTOSURE_FILES { ch_blacklist // channel: [optional] [path(blacklist)] main: - ch_versions = Channel.empty() + ch_versions = Channel.empty() + ch_reheader_out = Channel.empty() TIDDIT_COV_VCF2CYTOSURE (ch_bam, [[],[]]) @@ -24,24 +25,56 @@ workflow GENERATE_CYTOSURE_FILES { ch_vcf.join( ch_tbi, failOnMismatch: true ) .set { ch_vcf_tbi } - ch_bam.combine(ch_vcf_tbi).map { - meta_sample, bam, meta_case, vcf, tbi -> - new_meta = ['id':meta_sample.sample] - return [ new_meta, vcf, tbi ] - }.set { ch_sample_vcf } + ch_bam.combine(ch_vcf_tbi) + .map { + meta_sample, bam, meta_case, vcf, tbi -> + new_meta = ['id':meta_sample.sample] + return [ new_meta, vcf, tbi ] + } + .join(ch_sample_id_map, remainder: true) + .branch { it -> + id: it[3].equals(null) + return [it[0] + [custid:it[0].id], it[1], it[2]] + custid: !(it[3].equals(null)) + return [it[0] + [custid:it[3]], it[1], it[2]] + } + .set { ch_for_mix } + + Channel.empty() + .mix(ch_for_mix.id, ch_for_mix.custid) + .set { ch_sample_vcf } // Split vcf into sample vcf:s and frequency filter SPLIT_AND_FILTER_SV_VCF ( ch_sample_vcf, [], [], [] ) + if (params.sample_id_map != null) { + + SPLIT_AND_FILTER_SV_VCF.out.vcf + .map { meta, vcf -> return [meta, vcf, [], []]} + .set { ch_reheader_in } + + BCFTOOLS_REHEADER_SV_VCF ( ch_reheader_in, [[:],[]] ).vcf + .set {ch_reheader_out} + + ch_versions = ch_versions.mix(BCFTOOLS_REHEADER_SV_VCF.out.versions.first()) + } + SPLIT_AND_FILTER_SV_VCF.out.vcf - .join(ch_sample_id_map) - .map { meta, vcf, custid -> return [meta+[custid:custid], vcf, [], []]} - .set { ch_reheader_in } + .join(ch_reheader_out, remainder: true) + .branch { it -> + split: it[2].equals(null) + return [it[0], it[1]] + reheader: !(it[2].equals(null)) + return [it[0], it[2]] + } + .set { ch_for_mix } - BCFTOOLS_REHEADER_SV_VCF ( ch_reheader_in, [[:],[]] ) + Channel.empty() + .mix(ch_for_mix.split, ch_for_mix.reheader) + .set { ch_vcf2cytosure_in } VCF2CYTOSURE ( - BCFTOOLS_REHEADER_SV_VCF.out.vcf, + ch_vcf2cytosure_in, TIDDIT_COV_VCF2CYTOSURE.out.cov, [[:], []], [[:], []], ch_blacklist @@ -50,7 +83,6 @@ workflow GENERATE_CYTOSURE_FILES { ch_versions = ch_versions.mix(TIDDIT_COV_VCF2CYTOSURE.out.versions.first()) ch_versions = ch_versions.mix(SPLIT_AND_FILTER_SV_VCF.out.versions.first()) ch_versions = ch_versions.mix(VCF2CYTOSURE.out.versions.first()) - ch_versions = ch_versions.mix(BCFTOOLS_REHEADER_SV_VCF.out.versions.first()) emit: versions = ch_versions // channel: [ versions.yml ] diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index ae4eb986..47983d38 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -245,7 +245,7 @@ workflow RAREDISEASE { ch_rtg_truthvcfs = params.rtg_truthvcfs ? Channel.fromPath(params.rtg_truthvcfs).collect() : Channel.value([]) ch_sample_id_map = params.sample_id_map ? Channel.fromSamplesheet("sample_id_map") - : Channel.value([]) + : Channel.empty() ch_score_config_mt = params.score_config_mt ? Channel.fromPath(params.score_config_mt).collect() : Channel.value([]) ch_score_config_snv = params.score_config_snv ? Channel.fromPath(params.score_config_snv).collect() From 6c0a241a04fb5fd0c5713fd0cc286e99ac0dbb17 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 28 Feb 2024 23:25:14 +0100 Subject: [PATCH 354/387] add rename_align_files --- modules/local/rename_align_files.nf | 30 +++++++++++++++++++++++++++++ workflows/raredisease.nf | 23 +++++++++++++++------- 2 files changed, 46 insertions(+), 7 deletions(-) create mode 100644 modules/local/rename_align_files.nf diff --git a/modules/local/rename_align_files.nf b/modules/local/rename_align_files.nf new file mode 100644 index 00000000..da9f890b --- /dev/null +++ b/modules/local/rename_align_files.nf @@ -0,0 +1,30 @@ +process RENAME_ALIGN_FILES { + tag "$meta.id" + label 'process_single' + + conda "conda-forge::coreutils=8.31" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gnu-wget:1.18--0' : + 'biocontainers/gnu-wget:1.18--0' }" + + input: + tuple val(meta), path(input) + val(extension) + + output: + path("*.{bam,bai}"), emit: output + path "versions.yml", emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + ln -s $input ${meta.sample}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ln: \$(echo \$(ln --version 2>&1 | head -n 1 | cut -d ' ' -f4)) + END_VERSIONS + """ +} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 1a5b85e0..f6c8d3c5 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -101,9 +101,16 @@ if (missingParamsCount>0) { // MODULE: Installed directly from nf-core/modules // -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { SMNCOPYNUMBERCALLER } from '../modules/nf-core/smncopynumbercaller/main' +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { SMNCOPYNUMBERCALLER } from '../modules/nf-core/smncopynumbercaller/main' + +// +// MODULE: Local modules +// + +include { RENAME_ALIGN_FILES as RENAME_BAM_FOR_SMNCALLER } from '../modules/local/rename_align_files' +include { RENAME_ALIGN_FILES as RENAME_BAI_FOR_SMNCALLER } from '../modules/local/rename_align_files' // // SUBWORKFLOWS @@ -578,13 +585,13 @@ workflow RAREDISEASE { } // STEP 1.7: SMNCOPYNUMBERCALLER - ch_mapped.genome_bam_bai - .collect{it[1]} + RENAME_BAM_FOR_SMNCALLER(ch_mapped.genome_marked_bam, "bam").output + .collect{it} .toList() .set { ch_bam_list } - ch_mapped.genome_bam_bai - .collect{it[2]} + RENAME_BAI_FOR_SMNCALLER(ch_mapped.genome_marked_bai, "bam.bai").output + .collect{it} .toList() .set { ch_bai_list } @@ -596,6 +603,8 @@ workflow RAREDISEASE { SMNCOPYNUMBERCALLER ( ch_bams_bais ) + ch_versions = ch_versions.mix(RENAME_BAM_FOR_SMNCALLER.out.versions) + ch_versions = ch_versions.mix(RENAME_BAI_FOR_SMNCALLER.out.versions) ch_versions = ch_versions.mix(SMNCOPYNUMBERCALLER.out.versions) // ped correspondence, sex check, ancestry check From 82fbae9cb5f5c9bb56de38f14c35604f21bbc0e3 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 29 Feb 2024 01:38:39 +0100 Subject: [PATCH 355/387] chromograph autozyg --- conf/modules/annotate_rhocallviz.config | 11 +++++++++++ docs/output.md | 2 ++ subworkflows/local/annotation/annotate_rhocallviz.nf | 6 +++++- 3 files changed, 18 insertions(+), 1 deletion(-) diff --git a/conf/modules/annotate_rhocallviz.config b/conf/modules/annotate_rhocallviz.config index cfeb8d7e..bf33d35e 100644 --- a/conf/modules/annotate_rhocallviz.config +++ b/conf/modules/annotate_rhocallviz.config @@ -42,6 +42,17 @@ process { ] } + withName: '.*ANNOTATE_GENOME_SNVS:ANNOTATE_RHOCALLVIZ:CHROMOGRAPH_AUTOZYG' { + ext.prefix = { "${autozyg.simpleName}_autozyg_chromograph" } + ext.args = { "--euploid" } + ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun } + publishDir = [ + path: { "${params.outdir}/annotate_snv/genome/" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + withName: '.*ANNOTATE_GENOME_SNVS:ANNOTATE_RHOCALLVIZ:UCSC_WIGTOBIGWIG' { ext.prefix = { "${meta.sample}_rhocallviz" } ext.args = { "-clip" } diff --git a/docs/output.md b/docs/output.md index 281ac7dd..02258209 100644 --- a/docs/output.md +++ b/docs/output.md @@ -402,6 +402,8 @@ Based on VEP annotations, custom scripts used by the pipeline further annotate e - `_rohann_vcfanno_upd_sites_.png`: file containing a plot showing upd sites across chromosomes. - `annotate_snv/genome/*regions_chromograph` - `_rohann_vcfanno_upd_regions_.png`: file containing a plot showing upd regions across chromosomes. +- `annotate_snv/genome/*autozyg_chromograph` + - `_rhocallviz_.png`: file containing a plot showing regions of autozygosity across chromosomes. diff --git a/subworkflows/local/annotation/annotate_rhocallviz.nf b/subworkflows/local/annotation/annotate_rhocallviz.nf index 37aeb02d..c01eda52 100644 --- a/subworkflows/local/annotation/annotate_rhocallviz.nf +++ b/subworkflows/local/annotation/annotate_rhocallviz.nf @@ -8,6 +8,7 @@ include { BCFTOOLS_ROH } from '../../../modules/nf- include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_UNCOMPRESS } from '../../../modules/nf-core/bcftools/view/main' include { RHOCALL_VIZ } from '../../../modules/nf-core/rhocall/viz/main' include { UCSC_WIGTOBIGWIG } from '../../../modules/nf-core/ucsc/wigtobigwig/main' +include { CHROMOGRAPH as CHROMOGRAPH_AUTOZYG } from '../../../modules/nf-core/chromograph/main' workflow ANNOTATE_RHOCALLVIZ { @@ -36,11 +37,14 @@ workflow ANNOTATE_RHOCALLVIZ { BCFTOOLS_VIEW_UNCOMPRESS(ch_roh_in,[],[],[]) - RHOCALL_VIZ( BCFTOOLS_VIEW_UNCOMPRESS.out.vcf, BCFTOOLS_ROH.out.roh) + RHOCALL_VIZ(BCFTOOLS_VIEW_UNCOMPRESS.out.vcf, BCFTOOLS_ROH.out.roh) + + CHROMOGRAPH_AUTOZYG(RHOCALL_VIZ.out.bed, [[],[]], [[],[]], [[],[]], [[],[]], [[],[]], [[],[]]) UCSC_WIGTOBIGWIG(RHOCALL_VIZ.out.wig, ch_genome_chrsizes) ch_versions = ch_versions.mix(BCFTOOLS_VIEW.out.versions.first()) + ch_versions = ch_versions.mix(CHROMOGRAPH_AUTOZYG.out.versions.first()) ch_versions = ch_versions.mix(TABIX_TABIX.out.versions.first()) ch_versions = ch_versions.mix(BCFTOOLS_ROH.out.versions.first()) ch_versions = ch_versions.mix(BCFTOOLS_VIEW_UNCOMPRESS.out.versions.first()) From e44865eb090822c325975c44fe6eb3e70139ce9a Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 29 Feb 2024 10:22:45 +0100 Subject: [PATCH 356/387] add args --- conf/modules/call_repeat_expansions.config | 1 + 1 file changed, 1 insertion(+) diff --git a/conf/modules/call_repeat_expansions.config b/conf/modules/call_repeat_expansions.config index 39934a4b..7fbcdc4c 100644 --- a/conf/modules/call_repeat_expansions.config +++ b/conf/modules/call_repeat_expansions.config @@ -71,6 +71,7 @@ process { } withName: '.*CALL_REPEAT_EXPANSIONS:SPLIT_MULTIALLELICS_EXP' { + ext.args = '--output-type z --multiallelics -both' ext.prefix = { "${meta.id}_split_exp" } } From ae3307b3921a976a75e78a54a77c6202ecedeb3f Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 29 Feb 2024 16:11:36 +0000 Subject: [PATCH 357/387] Template update for nf-core/tools version 2.13.1 --- .devcontainer/devcontainer.json | 10 +---- .github/CONTRIBUTING.md | 14 ++++--- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/ci.yml | 2 +- .github/workflows/download_pipeline.yml | 2 +- .github/workflows/linting.yml | 2 +- .github/workflows/release-announcements.yml | 2 +- .gitpod.yml | 6 +-- README.md | 3 +- modules.json | 8 ++-- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- .../nf-core/multiqc/tests/main.nf.test.snap | 12 +++--- .../utils_nfcore_raredisease_pipeline/main.nf | 10 +++-- .../tests/main.function.nf.test | 2 +- .../tests/main.function.nf.test.snap | 12 +++++- .../tests/main.workflow.nf.test | 20 ++------- .../tests/nextflow.config | 2 +- .../tests/main.function.nf.test.snap | 42 +++++++++++++++---- .../tests/main.workflow.nf.test.snap | 6 ++- .../tests/main.nf.test | 2 +- 23 files changed, 99 insertions(+), 74 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe3..b290e090 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -10,15 +10,7 @@ "vscode": { // Set *default* container specific settings.json values on container create. "settings": { - "python.defaultInterpreterPath": "/opt/conda/bin/python", - "python.linting.enabled": true, - "python.linting.pylintEnabled": true, - "python.formatting.autopep8Path": "/opt/conda/bin/autopep8", - "python.formatting.yapfPath": "/opt/conda/bin/yapf", - "python.linting.flake8Path": "/opt/conda/bin/flake8", - "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", - "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.defaultInterpreterPath": "/opt/conda/bin/python" }, // Add the IDs of extensions you want installed when the container is created. diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 39013956..479c9fef 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,9 +9,8 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -:::info -If you need help using or modifying nf-core/raredisease then the best place to ask is on the nf-core Slack [#raredisease](https://nfcore.slack.com/channels/raredisease) channel ([join our Slack here](https://nf-co.re/join/slack)). -::: +> [!NOTE] +> If you need help using or modifying nf-core/raredisease then the best place to ask is on the nf-core Slack [#raredisease](https://nfcore.slack.com/channels/raredisease) channel ([join our Slack here](https://nf-co.re/join/slack)). ## Contribution workflow @@ -27,8 +26,11 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests -You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to -receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. +You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: + +```bash +nf-test test --profile debug,test,docker --verbose +``` When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -90,7 +92,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. ### Naming schemes diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index fc4dc8ad..23097c52 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rare - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/raredisease/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/raredisease _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 33080301..2919151f 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 8c3f5cb0..4ed98bab 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index e84295b2..e56bcd29 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -31,7 +31,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 with: version: "${{ matrix.NXF_VER }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index f823210d..08622fd5 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -28,7 +28,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 748b4311..073e1876 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -35,7 +35,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index c3674af2..d468aeaa 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -12,7 +12,7 @@ jobs: - name: get topics and convert to hashtags id: get_topics run: | - curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT - uses: rzr/fediverse-action@master with: diff --git a/.gitpod.yml b/.gitpod.yml index 363d5b1d..105a1821 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -10,13 +10,11 @@ tasks: vscode: extensions: # based on nf-core.nf-core-extensionpack - - codezombiech.gitignore # Language support for .gitignore files - # - cssho.vscode-svgviewer # SVG viewer - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code - - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar - mechatroner.rainbow-csv # Highlight columns in csv files in different colors - # - nextflow.nextflow # Nextflow syntax highlighting + # - nextflow.nextflow # Nextflow syntax highlighting - oderwat.indent-rainbow # Highlight indentation level - streetsidesoftware.code-spell-checker # Spelling checker for source code + - charliermarsh.ruff # Code linter Ruff diff --git a/README.md b/README.md index 2afc405b..bf3b4c0c 100644 --- a/README.md +++ b/README.md @@ -7,12 +7,13 @@ [![GitHub Actions CI Status](https://github.com/nf-core/raredisease/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/raredisease/actions/workflows/ci.yml) [![GitHub Actions Linting Status](https://github.com/nf-core/raredisease/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/raredisease/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/raredisease/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/raredisease) +[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/raredisease) [![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23raredisease-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/raredisease)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) diff --git a/modules.json b/modules.json index 06b7b0d7..b773a78a 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", "installed_by": ["modules"] } } @@ -21,17 +21,17 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] } } diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 2212096a..ca39fb67 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.20 + - bioconda::multiqc=1.21 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 354f4430..47ac352f 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : - 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : + 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index c204b488..bfebd802 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:28:51.744211298" + "timestamp": "2024-02-29T08:48:55.657331" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:28.847433492" + "timestamp": "2024-02-29T08:49:49.071937" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:13.223621555" + "timestamp": "2024-02-29T08:49:25.457567" } } \ No newline at end of file diff --git a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf index dc90ad90..3ec266c7 100644 --- a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf @@ -1,5 +1,5 @@ // -// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// Subworkflow with functionality specific to the nf-core/raredisease pipeline // /* @@ -152,7 +152,9 @@ workflow PIPELINE_COMPLETION { // def validateInputParameters() { genomeExistsError() -}// +} + +// // Validate channels from input samplesheet // def validateInputSamplesheet(input) { @@ -190,7 +192,9 @@ def genomeExistsError() { "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" error(error_string) } -}// +} + +// // Generate methods description for MultiQC // def toolCitationText() { diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test index 8ed4310c..68718e4f 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -51,4 +51,4 @@ nextflow_function { ) } } -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap index db2030f8..e3f0baf4 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -3,10 +3,18 @@ "content": [ "v9.9.9" ], - "timestamp": "2024-01-19T11:32:36.031083" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:05.308243" }, "Test Function checkCondaChannels": { "content": null, - "timestamp": "2024-01-19T11:32:50.456" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:12.425833" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test index f7c54bc6..ca964ce8 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -11,9 +11,6 @@ nextflow_workflow { test("Should run no inputs") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = false @@ -39,9 +36,6 @@ nextflow_workflow { test("Should print version") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = true @@ -68,19 +62,16 @@ nextflow_workflow { test("Should dump params") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = 'results' check_conda_channels = false input[0] = false input[1] = true - input[2] = params.outdir + input[2] = outdir input[3] = false """ } @@ -96,19 +87,16 @@ nextflow_workflow { test("Should not create params JSON if no output directory") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = null check_conda_channels = false input[0] = false input[1] = true - input[2] = null + input[2] = outdir input[3] = false """ } diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index 53574ffe..d0a926bf 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -6,4 +6,4 @@ manifest { nextflowVersion = '!>=23.04.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap index 10f948e6..1037232c 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -1,25 +1,41 @@ { "Test Function checkProfileProvided": { "content": null, - "timestamp": "2024-02-09T15:43:55.145717" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:03.360873" }, "Test Function checkConfigProvided": { "content": [ true ], - "timestamp": "2024-01-19T11:34:13.548431224" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:59.729647" }, "Test Function nfCoreLogo": { "content": [ "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" ], - "timestamp": "2024-01-19T11:34:38.840454873" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:10.562934" }, "Test Function workflowCitation": { "content": [ "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" ], - "timestamp": "2024-01-19T11:34:22.24352016" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:07.019761" }, "Test Function without logColours": { "content": [ @@ -73,13 +89,21 @@ "biwhite": "" } ], - "timestamp": "2024-01-19T11:35:04.418416984" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:17.969323" }, "Test Function dashedLine": { "content": [ "-\u001b[2m----------------------------------------------------\u001b[0m-" ], - "timestamp": "2024-01-19T11:34:55.420000755" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:14.366181" }, "Test Function with logColours": { "content": [ @@ -133,6 +157,10 @@ "biwhite": "\u001b[1;97m" } ], - "timestamp": "2024-01-19T11:35:13.436366565" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:21.714424" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap index d07ce54c..859d1030 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -10,6 +10,10 @@ ] } ], - "timestamp": "2024-01-19T11:35:22.538940073" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:25.726491" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test index 517ee54e..5784a33f 100644 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -197,4 +197,4 @@ nextflow_workflow { ) } } -} \ No newline at end of file +} From a7ef5c91822cb11fcaa1660a4d887f5bb59030bf Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 1 Mar 2024 11:21:42 +0100 Subject: [PATCH 358/387] review suggestion --- subworkflows/local/generate_cytosure_files.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/generate_cytosure_files.nf b/subworkflows/local/generate_cytosure_files.nf index 0f480a02..70ec8e81 100644 --- a/subworkflows/local/generate_cytosure_files.nf +++ b/subworkflows/local/generate_cytosure_files.nf @@ -28,7 +28,7 @@ workflow GENERATE_CYTOSURE_FILES { ch_bam.combine(ch_vcf_tbi) .map { meta_sample, bam, meta_case, vcf, tbi -> - new_meta = ['id':meta_sample.sample] + new_meta = ['id':meta_sample.sample, 'sex':meta_sample.sex] return [ new_meta, vcf, tbi ] } .join(ch_sample_id_map, remainder: true) From 7edad86e843e916164bc0d39a8dcbc9c479ba893 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 1 Mar 2024 13:25:05 +0100 Subject: [PATCH 359/387] update files unchanged --- .github/CONTRIBUTING.md | 2 +- .nf-core.yml | 1 + 2 files changed, 2 insertions(+), 1 deletion(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 479c9fef..5b0c2c80 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -29,7 +29,7 @@ If you're not used to this workflow with git, you can start with some [docs from You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: ```bash -nf-test test --profile debug,test,docker --verbose +nextflow run . -profile debug,test,docker --outdir ``` When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. diff --git a/.nf-core.yml b/.nf-core.yml index 629a06e7..285d7803 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -2,6 +2,7 @@ lint: files_exist: - conf/modules.config files_unchanged: + - .github/CONTRIBUTING.md - .github/PULL_REQUEST_TEMPLATE.md - docs/images/nf-core-raredisease_logo_dark.png - docs/images/nf-core-raredisease_logo_light.png From ccfa0aa117cb7163e3be0757e18706390ee23fce Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 4 Mar 2024 10:04:25 +0100 Subject: [PATCH 360/387] modules update --- modules.json | 82 +-- modules/nf-core/bwa/index/tests/main.nf.test | 4 +- modules/nf-core/bwa/mem/environment.yml | 3 +- modules/nf-core/bwa/mem/main.nf | 4 +- modules/nf-core/bwa/mem/tests/main.nf.test | 21 +- .../nf-core/bwa/mem/tests/main.nf.test.snap | 56 ++- modules/nf-core/bwamem2/mem/environment.yml | 3 +- modules/nf-core/bwamem2/mem/main.nf | 4 +- .../nf-core/bwamem2/mem/tests/main.nf.test | 62 ++- .../bwamem2/mem/tests/main.nf.test.snap | 40 +- modules/nf-core/cat/cat/tests/main.nf.test | 29 +- .../nf-core/cat/cat/tests/main.nf.test.snap | 92 ++-- modules/nf-core/fastp/tests/main.nf.test | 30 +- modules/nf-core/fastp/tests/main.nf.test.snap | 257 +++++++++- .../gatk4/bedtointervallist/environment.yml | 2 +- .../nf-core/gatk4/bedtointervallist/main.nf | 4 +- .../gatk4/collectreadcounts/environment.yml | 2 +- .../nf-core/gatk4/collectreadcounts/main.nf | 4 +- .../createsequencedictionary/environment.yml | 2 +- .../gatk4/createsequencedictionary/main.nf | 4 +- .../gatk4/denoisereadcounts/environment.yml | 2 +- .../nf-core/gatk4/denoisereadcounts/main.nf | 4 +- .../denoisereadcounts/tests/main.nf.test | 75 +++ .../denoisereadcounts/tests/main.nf.test.snap | 29 ++ .../denoisereadcounts/tests/nextflow.config | 9 + .../gatk4/denoisereadcounts/tests/tags.yml | 6 + .../environment.yml | 5 - .../determinegermlinecontigploidy/main.nf | 4 +- .../gatk4/filtermutectcalls/environment.yml | 2 +- .../nf-core/gatk4/filtermutectcalls/main.nf | 4 +- .../gatk4/germlinecnvcaller/environment.yml | 5 - .../nf-core/gatk4/germlinecnvcaller/main.nf | 4 +- .../gatk4/intervallisttools/environment.yml | 2 +- .../nf-core/gatk4/intervallisttools/main.nf | 4 +- .../gatk4/mergebamalignment/environment.yml | 2 +- .../nf-core/gatk4/mergebamalignment/main.nf | 4 +- .../nf-core/gatk4/mergevcfs/environment.yml | 2 +- modules/nf-core/gatk4/mergevcfs/main.nf | 4 +- modules/nf-core/gatk4/mergevcfs/meta.yml | 2 +- .../gatk4/mergevcfs/tests/main.nf.test | 87 ++++ .../gatk4/mergevcfs/tests/main.nf.test.snap | 35 ++ .../nf-core/gatk4/mergevcfs/tests/tags.yml | 2 + modules/nf-core/gatk4/mutect2/environment.yml | 2 +- modules/nf-core/gatk4/mutect2/main.nf | 4 +- .../environment.yml | 5 - .../gatk4/postprocessgermlinecnvcalls/main.nf | 4 +- .../gatk4/preprocessintervals/environment.yml | 2 +- .../nf-core/gatk4/preprocessintervals/main.nf | 4 +- .../nf-core/gatk4/printreads/environment.yml | 2 +- modules/nf-core/gatk4/printreads/main.nf | 4 +- .../nf-core/gatk4/revertsam/environment.yml | 2 +- modules/nf-core/gatk4/revertsam/main.nf | 4 +- .../nf-core/gatk4/samtofastq/environment.yml | 2 +- modules/nf-core/gatk4/samtofastq/main.nf | 4 +- .../gatk4/selectvariants/environment.yml | 2 +- modules/nf-core/gatk4/selectvariants/main.nf | 4 +- .../nf-core/gatk4/shiftfasta/environment.yml | 2 +- modules/nf-core/gatk4/shiftfasta/main.nf | 4 +- .../gatk4/splitintervals/environment.yml | 2 +- modules/nf-core/gatk4/splitintervals/main.nf | 4 +- .../gatk4/variantfiltration/environment.yml | 2 +- .../nf-core/gatk4/variantfiltration/main.nf | 4 +- .../nf-core/samtools/faidx/environment.yml | 3 +- modules/nf-core/samtools/faidx/main.nf | 4 +- .../nf-core/samtools/index/environment.yml | 3 +- modules/nf-core/samtools/index/main.nf | 4 +- .../nf-core/samtools/index/tests/main.nf.test | 36 +- .../samtools/index/tests/main.nf.test.snap | 54 +- .../nf-core/samtools/merge/environment.yml | 3 +- modules/nf-core/samtools/merge/main.nf | 4 +- .../nf-core/samtools/merge/tests/main.nf.test | 125 ++--- .../samtools/merge/tests/main.nf.test.snap | 220 +++++++-- modules/nf-core/samtools/sort/environment.yml | 3 +- modules/nf-core/samtools/sort/main.nf | 4 +- .../nf-core/samtools/sort/tests/main.nf.test | 33 +- .../samtools/sort/tests/main.nf.test.snap | 47 +- .../nf-core/samtools/stats/environment.yml | 4 +- modules/nf-core/samtools/stats/main.nf | 4 +- .../nf-core/samtools/stats/tests/main.nf.test | 53 +- .../samtools/stats/tests/main.nf.test.snap | 32 +- modules/nf-core/samtools/view/environment.yml | 3 +- modules/nf-core/samtools/view/main.nf | 4 +- .../nf-core/samtools/view/tests/main.nf.test | 193 ++++---- .../samtools/view/tests/main.nf.test.snap | 466 +++++++++++++++--- modules/nf-core/sentieon/bwaindex/main.nf | 4 +- modules/nf-core/sentieon/bwamem/main.nf | 4 +- modules/nf-core/sentieon/datametrics/main.nf | 4 +- modules/nf-core/sentieon/dedup/main.nf | 4 +- .../nf-core/sentieon/dnamodelapply/main.nf | 4 +- modules/nf-core/sentieon/dnascope/main.nf | 4 +- modules/nf-core/sentieon/readwriter/main.nf | 4 +- modules/nf-core/sentieon/wgsmetrics/main.nf | 4 +- modules/nf-core/svdb/merge/environment.yml | 3 +- modules/nf-core/svdb/merge/main.nf | 4 +- modules/nf-core/svdb/merge/meta.yml | 2 +- modules/nf-core/svdb/merge/tests/main.nf.test | 18 +- modules/nf-core/untar/tests/main.nf.test | 8 - modules/nf-core/untar/tests/main.nf.test.snap | 12 +- 98 files changed, 1729 insertions(+), 713 deletions(-) create mode 100644 modules/nf-core/gatk4/denoisereadcounts/tests/main.nf.test create mode 100644 modules/nf-core/gatk4/denoisereadcounts/tests/main.nf.test.snap create mode 100644 modules/nf-core/gatk4/denoisereadcounts/tests/nextflow.config create mode 100644 modules/nf-core/gatk4/denoisereadcounts/tests/tags.yml delete mode 100644 modules/nf-core/gatk4/determinegermlinecontigploidy/environment.yml delete mode 100644 modules/nf-core/gatk4/germlinecnvcaller/environment.yml create mode 100644 modules/nf-core/gatk4/mergevcfs/tests/main.nf.test create mode 100644 modules/nf-core/gatk4/mergevcfs/tests/main.nf.test.snap create mode 100644 modules/nf-core/gatk4/mergevcfs/tests/tags.yml delete mode 100644 modules/nf-core/gatk4/postprocessgermlinecnvcalls/environment.yml diff --git a/modules.json b/modules.json index 370d1472..54d8ed01 100644 --- a/modules.json +++ b/modules.json @@ -57,12 +57,12 @@ }, "bwa/index": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "6278bf9afd4a4b2d00fa6052250e73da3d91546f", "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "git_sha": "5908e575322666ccc33911a28b06e3f82260fe54", "installed_by": ["modules"] }, "bwamem2/index": { @@ -72,7 +72,7 @@ }, "bwamem2/mem": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "git_sha": "74363e1acc38eaedeede8d429477397c1a6f9e18", "installed_by": ["modules"] }, "cadd": { @@ -82,7 +82,7 @@ }, "cat/cat": { "branch": "master", - "git_sha": "81f27e75847087865299cc46605deb3b09b4e0a2", + "git_sha": "9437e6053dccf4aafa022bfd6e7e9de67e625af8", "installed_by": ["modules"] }, "chromograph": { @@ -128,7 +128,7 @@ }, "fastp": { "branch": "master", - "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", + "git_sha": "003920c7f9a8ae19b69a97171922880220bedf56", "installed_by": ["modules"] }, "fastqc": { @@ -138,104 +138,104 @@ }, "gatk4/bedtointervallist": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/collectreadcounts": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/createsequencedictionary": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/denoisereadcounts": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/determinegermlinecontigploidy": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "87e46f8fe8b056486a80c14b1d61e7cd6046bc06", "installed_by": ["modules"] }, "gatk4/filtermutectcalls": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/germlinecnvcaller": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "87e46f8fe8b056486a80c14b1d61e7cd6046bc06", "installed_by": ["modules"], "patch": "modules/nf-core/gatk4/germlinecnvcaller/gatk4-germlinecnvcaller.diff" }, "gatk4/intervallisttools": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/mergebamalignment": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/mergevcfs": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "194fca815cf594646e638fa5476acbcc296f1850", "installed_by": ["modules"] }, "gatk4/mutect2": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/postprocessgermlinecnvcalls": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "87e46f8fe8b056486a80c14b1d61e7cd6046bc06", "installed_by": ["modules"], "patch": "modules/nf-core/gatk4/postprocessgermlinecnvcalls/gatk4-postprocessgermlinecnvcalls.diff" }, "gatk4/preprocessintervals": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/printreads": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/revertsam": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/samtofastq": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/selectvariants": { "branch": "master", - "git_sha": "47cd015ac64f0294ae65a589693604127132a686", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/shiftfasta": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/splitintervals": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "gatk4/variantfiltration": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980", "installed_by": ["modules"] }, "genmod/annotate": { @@ -371,72 +371,72 @@ }, "samtools/faidx": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", "installed_by": ["modules"] }, "samtools/index": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", "installed_by": ["modules"] }, "samtools/merge": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", "installed_by": ["modules"] }, "samtools/sort": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", "installed_by": ["modules"] }, "samtools/stats": { "branch": "master", - "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", + "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", "installed_by": ["modules"] }, "samtools/view": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "git_sha": "0bd7d2333a88483aa0476acea172e9f5f6dd83bb", "installed_by": ["modules"] }, "sentieon/bwaindex": { "branch": "master", - "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", + "git_sha": "220da1aa7d6ab6555817035041dd2fc05cb518d3", "installed_by": ["modules"] }, "sentieon/bwamem": { "branch": "master", - "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", + "git_sha": "220da1aa7d6ab6555817035041dd2fc05cb518d3", "installed_by": ["modules"] }, "sentieon/datametrics": { "branch": "master", - "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", + "git_sha": "220da1aa7d6ab6555817035041dd2fc05cb518d3", "installed_by": ["modules"] }, "sentieon/dedup": { "branch": "master", - "git_sha": "740119f83a28777fdd846da87196b6ebc32a0e1d", + "git_sha": "220da1aa7d6ab6555817035041dd2fc05cb518d3", "installed_by": ["modules"] }, "sentieon/dnamodelapply": { "branch": "master", - "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", + "git_sha": "220da1aa7d6ab6555817035041dd2fc05cb518d3", "installed_by": ["modules"] }, "sentieon/dnascope": { "branch": "master", - "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", + "git_sha": "220da1aa7d6ab6555817035041dd2fc05cb518d3", "installed_by": ["modules"] }, "sentieon/readwriter": { "branch": "master", - "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", + "git_sha": "220da1aa7d6ab6555817035041dd2fc05cb518d3", "installed_by": ["modules"] }, "sentieon/wgsmetrics": { "branch": "master", - "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff", + "git_sha": "220da1aa7d6ab6555817035041dd2fc05cb518d3", "installed_by": ["modules"] }, "smncopynumbercaller": { @@ -451,7 +451,7 @@ }, "svdb/merge": { "branch": "master", - "git_sha": "d9c95c45db561814ff6c46a0c0574581e81beaae", + "git_sha": "ba3f3df395d2719dcef5c67189042a1dc555c701", "installed_by": ["modules"] }, "svdb/query": { @@ -491,7 +491,7 @@ }, "untar": { "branch": "master", - "git_sha": "e719354ba77df0a1bd310836aa2039b45c29d620", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["modules"] }, "upd": { diff --git a/modules/nf-core/bwa/index/tests/main.nf.test b/modules/nf-core/bwa/index/tests/main.nf.test index 5fc8d496..af33e73c 100644 --- a/modules/nf-core/bwa/index/tests/main.nf.test +++ b/modules/nf-core/bwa/index/tests/main.nf.test @@ -15,7 +15,7 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } @@ -29,5 +29,5 @@ nextflow_process { } } - + } diff --git a/modules/nf-core/bwa/mem/environment.yml b/modules/nf-core/bwa/mem/environment.yml index c5b2a9ce..3f136d0a 100644 --- a/modules/nf-core/bwa/mem/environment.yml +++ b/modules/nf-core/bwa/mem/environment.yml @@ -6,4 +6,5 @@ channels: dependencies: - bwa=0.7.17 # renovate: datasource=conda depName=bioconda/samtools - - samtools=1.18 + - samtools=1.19.2 + - htslib=1.19.1 diff --git a/modules/nf-core/bwa/mem/main.nf b/modules/nf-core/bwa/mem/main.nf index a3a82994..54ec0f16 100644 --- a/modules/nf-core/bwa/mem/main.nf +++ b/modules/nf-core/bwa/mem/main.nf @@ -4,8 +4,8 @@ process BWA_MEM { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:9c0128851101dafef65cef649826d2dbe6bedd7e-0' : - 'biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:9c0128851101dafef65cef649826d2dbe6bedd7e-0' }" + 'https://depot.galaxyproject.org/singularity/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:a34558545ae1413d94bde4578787ebef08027945-0' : + 'biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:a34558545ae1413d94bde4578787ebef08027945-0' }" input: tuple val(meta), path(reads) diff --git a/modules/nf-core/bwa/mem/tests/main.nf.test b/modules/nf-core/bwa/mem/tests/main.nf.test index b199bb70..2696e4bf 100644 --- a/modules/nf-core/bwa/mem/tests/main.nf.test +++ b/modules/nf-core/bwa/mem/tests/main.nf.test @@ -5,6 +5,7 @@ nextflow_process { tag "modules" tag "bwa" tag "bwa/mem" + tag "bwa/index" script "../main.nf" process "BWA_MEM" @@ -17,7 +18,7 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } @@ -30,7 +31,7 @@ nextflow_process { input[0] = [ [ id:'test', single_end:true ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] ] input[1] = BWA_INDEX.out.index @@ -57,7 +58,7 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } @@ -70,7 +71,7 @@ nextflow_process { input[0] = [ [ id:'test', single_end:true ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] ] input[1] = BWA_INDEX.out.index @@ -97,7 +98,7 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } @@ -110,8 +111,8 @@ nextflow_process { input[0] = [ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] ] input[1] = BWA_INDEX.out.index @@ -138,7 +139,7 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } @@ -151,8 +152,8 @@ nextflow_process { input[0] = [ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] ] input[1] = BWA_INDEX.out.index diff --git a/modules/nf-core/bwa/mem/tests/main.nf.test.snap b/modules/nf-core/bwa/mem/tests/main.nf.test.snap index bfb55fc7..e4fd8cc0 100644 --- a/modules/nf-core/bwa/mem/tests/main.nf.test.snap +++ b/modules/nf-core/bwa/mem/tests/main.nf.test.snap @@ -8,11 +8,11 @@ "id": "test", "single_end": true }, - "test.bam:md5,df203d7c7e8fef351408a909570c7952" + "test.bam:md5,a74710a0345b4717bb4431bf9c257120" ] ], "1": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" ], "bam": [ [ @@ -20,15 +20,19 @@ "id": "test", "single_end": true }, - "test.bam:md5,df203d7c7e8fef351408a909570c7952" + "test.bam:md5,a74710a0345b4717bb4431bf9c257120" ] ], "versions": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" ] } ], - "timestamp": "2023-12-04T11:01:22.483594641" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-19T11:11:48.440661587" }, "Single-End Sort": { "content": [ @@ -39,11 +43,11 @@ "id": "test", "single_end": true }, - "test.bam:md5,8a52bd78fdcecb994c1f63897d5b431c" + "test.bam:md5,cb1e038bc4d990683fa485d632550b54" ] ], "1": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" ], "bam": [ [ @@ -51,15 +55,19 @@ "id": "test", "single_end": true }, - "test.bam:md5,8a52bd78fdcecb994c1f63897d5b431c" + "test.bam:md5,cb1e038bc4d990683fa485d632550b54" ] ], "versions": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" ] } ], - "timestamp": "2023-12-04T11:01:30.180783483" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-19T11:11:56.086493265" }, "Paired-End": { "content": [ @@ -70,11 +78,11 @@ "id": "test", "single_end": false }, - "test.bam:md5,9815aef9ec763a60c53c3879be2d73ae" + "test.bam:md5,aea123a3828a99da1906126355f15a12" ] ], "1": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" ], "bam": [ [ @@ -82,15 +90,19 @@ "id": "test", "single_end": false }, - "test.bam:md5,9815aef9ec763a60c53c3879be2d73ae" + "test.bam:md5,aea123a3828a99da1906126355f15a12" ] ], "versions": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" ] } ], - "timestamp": "2023-12-04T11:01:38.761983007" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-19T11:12:03.474974773" }, "Paired-End Sort": { "content": [ @@ -101,11 +113,11 @@ "id": "test", "single_end": false }, - "test.bam:md5,0f0cda73704c4f7ba08af482edcbbe88" + "test.bam:md5,4682087bcdc3617384b375093fecd8dd" ] ], "1": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" ], "bam": [ [ @@ -113,14 +125,18 @@ "id": "test", "single_end": false }, - "test.bam:md5,0f0cda73704c4f7ba08af482edcbbe88" + "test.bam:md5,4682087bcdc3617384b375093fecd8dd" ] ], "versions": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" ] } ], - "timestamp": "2023-12-04T11:01:46.284587802" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-19T11:12:10.721510817" } } \ No newline at end of file diff --git a/modules/nf-core/bwamem2/mem/environment.yml b/modules/nf-core/bwamem2/mem/environment.yml index 227d8690..cbf06d39 100644 --- a/modules/nf-core/bwamem2/mem/environment.yml +++ b/modules/nf-core/bwamem2/mem/environment.yml @@ -6,4 +6,5 @@ channels: dependencies: - bwa-mem2=2.2.1 # renovate: datasource=conda depName=bioconda/samtools - - samtools=1.18 + - samtools=1.19.2 + - htslib=1.19.1 diff --git a/modules/nf-core/bwamem2/mem/main.nf b/modules/nf-core/bwamem2/mem/main.nf index b3874fc9..29f90778 100644 --- a/modules/nf-core/bwamem2/mem/main.nf +++ b/modules/nf-core/bwamem2/mem/main.nf @@ -4,8 +4,8 @@ process BWAMEM2_MEM { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:6351200f24497efba12c219c2bea4bb0f69a9d47-0' : - 'biocontainers/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:6351200f24497efba12c219c2bea4bb0f69a9d47-0' }" + 'https://depot.galaxyproject.org/singularity/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:2d15960ccea84e249a150b7f5d4db3a42fc2d6c3-0' : + 'biocontainers/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:2d15960ccea84e249a150b7f5d4db3a42fc2d6c3-0' }" input: tuple val(meta), path(reads) diff --git a/modules/nf-core/bwamem2/mem/tests/main.nf.test b/modules/nf-core/bwamem2/mem/tests/main.nf.test index d7912069..365a0c43 100644 --- a/modules/nf-core/bwamem2/mem/tests/main.nf.test +++ b/modules/nf-core/bwamem2/mem/tests/main.nf.test @@ -8,6 +8,7 @@ nextflow_process { tag "modules_nfcore" tag "bwamem2" tag "bwamem2/mem" + tag "bwamem2/index" test("sarscov2 - fastq, index, false") { @@ -16,7 +17,10 @@ nextflow_process { script "../../index/main.nf" process { """ - input[0] = [ [:], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) """ } } @@ -25,10 +29,10 @@ nextflow_process { when { process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:true ], // meta map - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] + [file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)] + ]) input[1] = BWAMEM2_INDEX.out.index input[2] = false """ @@ -54,7 +58,10 @@ nextflow_process { script "../../index/main.nf" process { """ - input[0] = [ [:], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) """ } } @@ -63,10 +70,10 @@ nextflow_process { when { process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:true ], // meta map - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] + [file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)] + ]) input[1] = BWAMEM2_INDEX.out.index input[2] = true """ @@ -92,7 +99,10 @@ nextflow_process { script "../../index/main.nf" process { """ - input[0] = [ [:], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) """ } } @@ -101,13 +111,13 @@ nextflow_process { when { process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ] + ]) input[1] = BWAMEM2_INDEX.out.index input[2] = false """ @@ -133,7 +143,10 @@ nextflow_process { script "../../index/main.nf" process { """ - input[0] = [ [:], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) """ } } @@ -142,13 +155,13 @@ nextflow_process { when { process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ] + ]) input[1] = BWAMEM2_INDEX.out.index input[2] = true """ @@ -176,7 +189,10 @@ nextflow_process { script "../../index/main.nf" process { """ - input[0] = [ [:], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) """ } } @@ -185,13 +201,13 @@ nextflow_process { when { process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ] + ]) input[1] = BWAMEM2_INDEX.out.index input[2] = true """ diff --git a/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap b/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap index db7dd392..84be71c6 100644 --- a/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap +++ b/modules/nf-core/bwamem2/mem/tests/main.nf.test.snap @@ -3,45 +3,65 @@ "content": [ "test.bam", [ - "versions.yml:md5,f653bf29c6597481cd0345bd66aafd8a" + "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" ] ], - "timestamp": "2023-12-04T11:49:07.89544697" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-19T13:30:22.691288603" }, "sarscov2 - [fastq1, fastq2], index, false": { "content": [ "test.bam", [ - "versions.yml:md5,f653bf29c6597481cd0345bd66aafd8a" + "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" ] ], - "timestamp": "2023-12-04T11:48:56.319355195" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-19T13:30:11.276168706" }, "sarscov2 - [fastq1, fastq2], index, true - stub": { "content": [ "test.bam", [ - "versions.yml:md5,f653bf29c6597481cd0345bd66aafd8a" + "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" ] ], - "timestamp": "2023-12-04T11:49:17.499101941" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-19T13:30:32.07431961" }, "sarscov2 - fastq, index, false": { "content": [ "test.bam", [ - "versions.yml:md5,f653bf29c6597481cd0345bd66aafd8a" + "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" ] ], - "timestamp": "2023-12-04T11:48:31.351628851" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-19T13:29:48.586760544" }, "sarscov2 - fastq, index, true": { "content": [ "test.bam", [ - "versions.yml:md5,f653bf29c6597481cd0345bd66aafd8a" + "versions.yml:md5,1c1a9566f189ec077b5179bbf453c51a" ] ], - "timestamp": "2023-12-04T11:48:43.978452504" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-19T13:29:59.846686393" } } \ No newline at end of file diff --git a/modules/nf-core/cat/cat/tests/main.nf.test b/modules/nf-core/cat/cat/tests/main.nf.test index aaae04f9..fcee2d19 100644 --- a/modules/nf-core/cat/cat/tests/main.nf.test +++ b/modules/nf-core/cat/cat/tests/main.nf.test @@ -19,8 +19,8 @@ nextflow_process { [ [ id:'genome', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes', checkIfExists: true) ] ] """ @@ -45,8 +45,8 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes', checkIfExists: true) ] ] """ @@ -72,8 +72,8 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gff3.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/alignment/last/contigs.genome.maf.gz', checkIfExists: true) ] ] """ @@ -83,7 +83,8 @@ nextflow_process { def lines = path(process.out.file_out.get(0).get(1)).linesGzip assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot(lines[0..5]).match("test_cat_zipped_zipped_lines") }, + { assert snapshot(lines.size()).match("test_cat_zipped_zipped_size")} ) } } @@ -101,8 +102,8 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gff3.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/alignment/last/contigs.genome.maf.gz', checkIfExists: true) ] ] """ @@ -130,8 +131,8 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes', checkIfExists: true) ] ] """ @@ -141,7 +142,8 @@ nextflow_process { def lines = path(process.out.file_out.get(0).get(1)).linesGzip assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot(lines[0..5]).match("test_cat_unzipped_zipped_lines") }, + { assert snapshot(lines.size()).match("test_cat_unzipped_zipped_size")} ) } } @@ -158,7 +160,7 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] ] """ @@ -174,4 +176,3 @@ nextflow_process { } } } - diff --git a/modules/nf-core/cat/cat/tests/main.nf.test.snap b/modules/nf-core/cat/cat/tests/main.nf.test.snap index 0c9bfe8d..423571ba 100644 --- a/modules/nf-core/cat/cat/tests/main.nf.test.snap +++ b/modules/nf-core/cat/cat/tests/main.nf.test.snap @@ -1,4 +1,10 @@ { + "test_cat_unzipped_zipped_size": { + "content": [ + 375 + ], + "timestamp": "2023-10-16T14:33:08.049445686" + }, "test_cat_unzipped_unzipped": { "content": [ { @@ -61,36 +67,31 @@ ], "timestamp": "2023-10-16T14:32:49.642741302" }, - "test_cat_zipped_zipped": { + "test_cat_zipped_zipped_lines": { "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": true - }, - "test.gff3.gz:md5,c439d3b60e7bc03e8802a451a0d9a5d9" - ] - ], - "1": [ - "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" - ], - "file_out": [ - [ - { - "id": "test", - "single_end": true - }, - "test.gff3.gz:md5,c439d3b60e7bc03e8802a451a0d9a5d9" - ] - ], - "versions": [ - "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" - ] - } + [ + "MT192765.1\tGenbank\ttranscript\t259\t29667\t.\t+\t.\tID=unknown_transcript_1;geneID=orf1ab;gene_name=orf1ab", + "MT192765.1\tGenbank\tgene\t259\t21548\t.\t+\t.\tParent=unknown_transcript_1", + "MT192765.1\tGenbank\tCDS\t259\t13461\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1", + "MT192765.1\tGenbank\tCDS\t13461\t21548\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1", + "MT192765.1\tGenbank\tCDS\t21556\t25377\t.\t+\t0\tParent=unknown_transcript_1;gbkey=CDS;gene=S;note=\"structural protein\";product=\"surface glycoprotein\";protein_id=QIK50427.1", + "MT192765.1\tGenbank\tgene\t21556\t25377\t.\t+\t.\tParent=unknown_transcript_1" + ] + ], + "timestamp": "2023-10-16T14:32:33.629048645" + }, + "test_cat_unzipped_zipped_lines": { + "content": [ + [ + ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome", + "GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGT", + "GTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAG", + "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG", + "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT", + "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG" + ] ], - "timestamp": "2024-01-12T14:02:02.999254641" + "timestamp": "2023-10-16T14:33:08.038830506" }, "test_cat_one_file_unzipped_zipped_lines": { "content": [ @@ -105,41 +106,16 @@ ], "timestamp": "2023-10-16T14:33:21.39642399" }, - "test_cat_unzipped_zipped": { + "test_cat_zipped_zipped_size": { "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": true - }, - "cat.txt.gz:md5,f44b33a0e441ad58b2d3700270e2dbe2" - ] - ], - "1": [ - "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" - ], - "file_out": [ - [ - { - "id": "test", - "single_end": true - }, - "cat.txt.gz:md5,f44b33a0e441ad58b2d3700270e2dbe2" - ] - ], - "versions": [ - "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" - ] - } + 78 ], - "timestamp": "2024-01-12T14:08:26.948048418" + "timestamp": "2023-10-16T14:32:33.641869244" }, "test_cat_one_file_unzipped_zipped_size": { "content": [ 374 ], - "timestamp": "2024-01-12T14:10:22.445700266" + "timestamp": "2023-10-16T14:33:21.4094373" } -} +} \ No newline at end of file diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test index fa7e5b4d..9b3f9a38 100644 --- a/modules/nf-core/fastp/tests/main.nf.test +++ b/modules/nf-core/fastp/tests/main.nf.test @@ -67,9 +67,9 @@ nextflow_process { process.out.reads_fail.collect { file(it[1]).getName() } + process.out.reads_merged.collect { file(it[1]).getName() } ).sort() - ).match("test_fastp_single_end-for_stub_match") + ).match("test_fastp_single_end-_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_single_end") } ) } } @@ -116,7 +116,7 @@ nextflow_process { ).sort() ).match("test_fastp_single_end-for_stub_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_single_end_stub") } ) } } @@ -196,9 +196,9 @@ nextflow_process { process.out.reads_fail.collect { file(it[1]).getName() } + process.out.reads_merged.collect { file(it[1]).getName() } ).sort() - ).match("test_fastp_paired_end-for_stub_match") + ).match("test_fastp_paired_end_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_paired_end") } ) } } @@ -245,7 +245,7 @@ nextflow_process { ).sort() ).match("test_fastp_paired_end-for_stub_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_paired_end-stub") } ) } } @@ -310,9 +310,9 @@ nextflow_process { process.out.reads_fail.collect { file(it[1]).getName() } + process.out.reads_merged.collect { file(it[1]).getName() } ).sort() - ).match("test_fastp_interleaved-for_stub_match") + ).match("test_fastp_interleaved-_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_interleaved") } ) } } @@ -359,7 +359,7 @@ nextflow_process { ).sort() ).match("test_fastp_interleaved-for_stub_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_interleaved-stub") } ) } } @@ -420,7 +420,7 @@ nextflow_process { { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } } }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_single_end_trim_fail") } ) } } @@ -498,7 +498,7 @@ nextflow_process { { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } } }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_paired_end_trim_fail") } ) } } @@ -587,9 +587,9 @@ nextflow_process { process.out.reads_fail.collect { file(it[1]).getName() } + process.out.reads_merged.collect { file(it[1]).getName() } ).sort() - ).match("test_fastp_paired_end_merged-for_stub_match") + ).match("test_fastp_paired_end_merged_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_paired_end_merged") } ) } } @@ -636,7 +636,7 @@ nextflow_process { ).sort() ).match("test_fastp_paired_end_merged-for_stub_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_paired_end_merged_stub") } ) } } @@ -716,7 +716,7 @@ nextflow_process { { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } } }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_paired_end_merged_adapterlist") } ) } } diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap index 6a71b680..b4c0e1dd 100644 --- a/modules/nf-core/fastp/tests/main.nf.test.snap +++ b/modules/nf-core/fastp/tests/main.nf.test.snap @@ -1,19 +1,4 @@ { - "test_fastp_paired_end-for_stub_match": { - "content": [ - [ - [ - "test_1.fastp.fastq.gz", - "test_2.fastp.fastq.gz" - ], - "test.fastp.html", - "test.fastp.json", - "test.fastp.log", - "{id=test, single_end=false}" - ] - ], - "timestamp": "2024-01-17T18:07:15.398827" - }, "fastp test_fastp_interleaved_json": { "content": [ [ @@ -26,6 +11,10 @@ ] ] ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, "timestamp": "2024-01-17T18:08:06.123035" }, "test_fastp_paired_end_merged-for_stub_match": { @@ -42,8 +31,24 @@ "{id=test, single_end=false}" ] ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, "timestamp": "2024-01-17T18:10:13.467574" }, + "versions_interleaved": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:56:24.615634793" + }, "test_fastp_single_end_json": { "content": [ [ @@ -56,15 +61,169 @@ ] ] ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, "timestamp": "2024-01-17T18:06:00.223817" }, - "versions": { + "versions_paired_end": { "content": [ [ "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" ] ], - "timestamp": "2024-01-17T18:06:00.248422" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:55:42.333545689" + }, + "test_fastp_paired_end_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=false}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:03:06.431833729" + }, + "test_fastp_interleaved-_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:03:37.827323085" + }, + "test_fastp_paired_end_merged_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "test.merged.fastq.gz", + "{id=test, single_end=false}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:08:44.496251446" + }, + "versions_single_end_stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:55:27.354051299" + }, + "versions_interleaved-stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:56:46.535528418" + }, + "versions_single_end_trim_fail": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:03.724591407" + }, + "test_fastp_paired_end-for_stub_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=false}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:07:15.398827" + }, + "versions_paired_end-stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:56:06.50017282" + }, + "versions_single_end": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:55:07.67921647" + }, + "versions_paired_end_merged_stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:47.350653154" }, "test_fastp_interleaved-for_stub_match": { "content": [ @@ -76,8 +235,24 @@ "{id=test, single_end=true}" ] ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, "timestamp": "2024-01-17T18:08:06.127974" }, + "versions_paired_end_trim_fail": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:18.140484878" + }, "test_fastp_single_end-for_stub_match": { "content": [ [ @@ -88,8 +263,52 @@ "{id=test, single_end=true}" ] ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, "timestamp": "2024-01-17T18:06:00.244202" }, + "test_fastp_single_end-_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:57:30.791982648" + }, + "versions_paired_end_merged_adapterlist": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:05:37.845370554" + }, + "versions_paired_end_merged": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:32.860543858" + }, "test_fastp_single_end_trim_fail_json": { "content": [ [ @@ -102,6 +321,10 @@ ] ] ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, "timestamp": "2024-01-17T18:08:41.942317" } } \ No newline at end of file diff --git a/modules/nf-core/gatk4/bedtointervallist/environment.yml b/modules/nf-core/gatk4/bedtointervallist/environment.yml index e7cb4280..d6fbe2e7 100644 --- a/modules/nf-core/gatk4/bedtointervallist/environment.yml +++ b/modules/nf-core/gatk4/bedtointervallist/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/bedtointervallist/main.nf b/modules/nf-core/gatk4/bedtointervallist/main.nf index 88b24b1a..68863d67 100644 --- a/modules/nf-core/gatk4/bedtointervallist/main.nf +++ b/modules/nf-core/gatk4/bedtointervallist/main.nf @@ -4,8 +4,8 @@ process GATK4_BEDTOINTERVALLIST { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(bed) diff --git a/modules/nf-core/gatk4/collectreadcounts/environment.yml b/modules/nf-core/gatk4/collectreadcounts/environment.yml index ebc83800..d09cd890 100644 --- a/modules/nf-core/gatk4/collectreadcounts/environment.yml +++ b/modules/nf-core/gatk4/collectreadcounts/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/collectreadcounts/main.nf b/modules/nf-core/gatk4/collectreadcounts/main.nf index 1cc36941..2c545d22 100644 --- a/modules/nf-core/gatk4/collectreadcounts/main.nf +++ b/modules/nf-core/gatk4/collectreadcounts/main.nf @@ -4,8 +4,8 @@ process GATK4_COLLECTREADCOUNTS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(input), path(input_index), path(intervals) diff --git a/modules/nf-core/gatk4/createsequencedictionary/environment.yml b/modules/nf-core/gatk4/createsequencedictionary/environment.yml index db663e14..78822ad0 100644 --- a/modules/nf-core/gatk4/createsequencedictionary/environment.yml +++ b/modules/nf-core/gatk4/createsequencedictionary/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/createsequencedictionary/main.nf b/modules/nf-core/gatk4/createsequencedictionary/main.nf index b47ad162..c7f1d75b 100644 --- a/modules/nf-core/gatk4/createsequencedictionary/main.nf +++ b/modules/nf-core/gatk4/createsequencedictionary/main.nf @@ -4,8 +4,8 @@ process GATK4_CREATESEQUENCEDICTIONARY { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/gatk4/denoisereadcounts/environment.yml b/modules/nf-core/gatk4/denoisereadcounts/environment.yml index bcb2ff35..a03cf4a9 100644 --- a/modules/nf-core/gatk4/denoisereadcounts/environment.yml +++ b/modules/nf-core/gatk4/denoisereadcounts/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/denoisereadcounts/main.nf b/modules/nf-core/gatk4/denoisereadcounts/main.nf index b1191c27..878e85c6 100644 --- a/modules/nf-core/gatk4/denoisereadcounts/main.nf +++ b/modules/nf-core/gatk4/denoisereadcounts/main.nf @@ -4,8 +4,8 @@ process GATK4_DENOISEREADCOUNTS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(counts) diff --git a/modules/nf-core/gatk4/denoisereadcounts/tests/main.nf.test b/modules/nf-core/gatk4/denoisereadcounts/tests/main.nf.test new file mode 100644 index 00000000..35ad8f79 --- /dev/null +++ b/modules/nf-core/gatk4/denoisereadcounts/tests/main.nf.test @@ -0,0 +1,75 @@ +nextflow_process { + + name "Test Process GATK4_DENOISEREADCOUNTS" + script "../main.nf" + config "./nextflow.config" + process "GATK4_DENOISEREADCOUNTS" + + tag "modules" + tag "modules_nfcore" + tag "gatk4" + tag "gatk4/createreadcountpanelofnormals" + tag "gatk4/collectreadcounts" + tag "gatk4/preprocessintervals" + tag "gatk4/denoisereadcounts" + + test("test_gatk4_denoisereadcounts") { + setup { + run("GATK4_PREPROCESSINTERVALS"){ + script "../../preprocessintervals/main.nf" + process { + """ + input[0] = Channel.value([ [ id:'test' ], file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)]) + input[1] = Channel.value([ [ id:'test' ], file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true)]) + input[2] = Channel.value([ [ id:'test' ], file(params.test_data['homo_sapiens']['genome']['genome_dict'], checkIfExists: true)]) + input[3] = [[],[]] + input[4] = [[],[]] + """ + } + } + run("GATK4_COLLECTREADCOUNTS"){ + script "../../collectreadcounts/main.nf" + process { + """ + intervals = GATK4_PREPROCESSINTERVALS.out.interval_list.map {meta, list -> list} + input[0] = Channel.of( + [[ id:'test', single_end:false ], file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)], + ) + .combine( intervals) + input[1] = Channel.value([ [ id:'test' ], file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)]) + input[2] = Channel.value([ [ id:'test' ], file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true)]) + input[3] = Channel.value([ [ id:'test' ], file(params.test_data['homo_sapiens']['genome']['genome_dict'], checkIfExists: true)]) + """ + } + } + run("GATK4_CREATEREADCOUNTPANELOFNORMALS"){ + script "../../createreadcountpanelofnormals/main.nf" + process { + """ + input[0] = GATK4_COLLECTREADCOUNTS.out.tsv.groupTuple() + """ + } + } + } + + when { + process { + """ + input[0] = GATK4_COLLECTREADCOUNTS.out.tsv.first() + input[1] = GATK4_CREATEREADCOUNTPANELOFNORMALS.out.pon + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.standardized, + process.out.denoised + ).match() } + ) + } + + } +} diff --git a/modules/nf-core/gatk4/denoisereadcounts/tests/main.nf.test.snap b/modules/nf-core/gatk4/denoisereadcounts/tests/main.nf.test.snap new file mode 100644 index 00000000..000283b3 --- /dev/null +++ b/modules/nf-core/gatk4/denoisereadcounts/tests/main.nf.test.snap @@ -0,0 +1,29 @@ +{ + "test_gatk4_denoisereadcounts": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test_standardizedCR.tsv:md5,f9e56b8e12b4dadc91a6d977fa79c6a8" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test_denoisedCR.tsv:md5,f9e56b8e12b4dadc91a6d977fa79c6a8" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-20T14:45:41.834159" + } +} \ No newline at end of file diff --git a/modules/nf-core/gatk4/denoisereadcounts/tests/nextflow.config b/modules/nf-core/gatk4/denoisereadcounts/tests/nextflow.config new file mode 100644 index 00000000..e67bb684 --- /dev/null +++ b/modules/nf-core/gatk4/denoisereadcounts/tests/nextflow.config @@ -0,0 +1,9 @@ +docker.runOptions = '--platform=linux/amd64 -e "HOME=${HOME}" -v /etc/passwd:/etc/passwd:ro -v /etc/shadow:/etc/shadow:ro -v /etc/group:/etc/group:ro -v $HOME:$HOME' +process { + withName: GATK4_COLLECTREADCOUNTS { + ext.args = "--format TSV --interval-merging-rule OVERLAPPING_ONLY" + } + withName: GATK4_CREATEREADCOUNTPANELOFNORMALS { + ext.args = "--minimum-interval-median-percentile 1.0 --number-of-eigensamples 2" + } +} diff --git a/modules/nf-core/gatk4/denoisereadcounts/tests/tags.yml b/modules/nf-core/gatk4/denoisereadcounts/tests/tags.yml new file mode 100644 index 00000000..5f105a82 --- /dev/null +++ b/modules/nf-core/gatk4/denoisereadcounts/tests/tags.yml @@ -0,0 +1,6 @@ +gatk4/denoisereadcounts: + - "modules/nf-core/gatk4/denoisereadcounts/**" + - "modules/nf-core/gatk4/createreadcountpanelofnormals/**" + - "modules/nf-core/gatk4/collectreadcounts/**" + - "modules/nf-core/gatk4/preprocessintervals/**" + - "modules/nf-core/gatk4/denoisereadcounts/**" diff --git a/modules/nf-core/gatk4/determinegermlinecontigploidy/environment.yml b/modules/nf-core/gatk4/determinegermlinecontigploidy/environment.yml deleted file mode 100644 index c30de090..00000000 --- a/modules/nf-core/gatk4/determinegermlinecontigploidy/environment.yml +++ /dev/null @@ -1,5 +0,0 @@ -name: gatk4_determinegermlinecontigploidy -channels: - - conda-forge - - bioconda - - defaults diff --git a/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf b/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf index 5af101f8..71a67f70 100644 --- a/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf +++ b/modules/nf-core/gatk4/determinegermlinecontigploidy/main.nf @@ -4,7 +4,7 @@ process GATK4_DETERMINEGERMLINECONTIGPLOIDY { label 'process_single' //Conda is not supported at the moment: https://github.com/broadinstitute/gatk/issues/7811 - container "nf-core/gatk:4.4.0.0" //Biocontainers is missing a package + container "nf-core/gatk:4.5.0.0" //Biocontainers is missing a package input: tuple val(meta), path(counts), path(bed), path(exclude_beds) @@ -39,6 +39,8 @@ process GATK4_DETERMINEGERMLINECONTIGPLOIDY { avail_mem = (task.memory.mega*0.8).intValue() } """ + export THEANO_FLAGS="base_compiledir=\$PWD" + gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\ DetermineGermlineContigPloidy \\ ${input_list} \\ diff --git a/modules/nf-core/gatk4/filtermutectcalls/environment.yml b/modules/nf-core/gatk4/filtermutectcalls/environment.yml index 8057d765..7494d84d 100644 --- a/modules/nf-core/gatk4/filtermutectcalls/environment.yml +++ b/modules/nf-core/gatk4/filtermutectcalls/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/filtermutectcalls/main.nf b/modules/nf-core/gatk4/filtermutectcalls/main.nf index fa6b46ab..38dd44c7 100644 --- a/modules/nf-core/gatk4/filtermutectcalls/main.nf +++ b/modules/nf-core/gatk4/filtermutectcalls/main.nf @@ -4,8 +4,8 @@ process GATK4_FILTERMUTECTCALLS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(vcf), path(vcf_tbi), path(stats), path(orientationbias), path(segmentation), path(table), val(estimate) diff --git a/modules/nf-core/gatk4/germlinecnvcaller/environment.yml b/modules/nf-core/gatk4/germlinecnvcaller/environment.yml deleted file mode 100644 index 02ce4dd3..00000000 --- a/modules/nf-core/gatk4/germlinecnvcaller/environment.yml +++ /dev/null @@ -1,5 +0,0 @@ -name: gatk4_germlinecnvcaller -channels: - - conda-forge - - bioconda - - defaults diff --git a/modules/nf-core/gatk4/germlinecnvcaller/main.nf b/modules/nf-core/gatk4/germlinecnvcaller/main.nf index f42ceb6a..535b14c5 100644 --- a/modules/nf-core/gatk4/germlinecnvcaller/main.nf +++ b/modules/nf-core/gatk4/germlinecnvcaller/main.nf @@ -3,7 +3,7 @@ process GATK4_GERMLINECNVCALLER { label 'process_single' //Conda is not supported at the moment: https://github.com/broadinstitute/gatk/issues/7811 - container "nf-core/gatk:4.4.0.0" //Biocontainers is missing a package + container "nf-core/gatk:4.5.0.0" //Biocontainers is missing a package input: tuple val(meta), path(tsv), path(intervals), path(ploidy), path(model) @@ -37,6 +37,8 @@ process GATK4_GERMLINECNVCALLER { avail_mem = (task.memory.mega*0.8).intValue() } """ + export THEANO_FLAGS="base_compiledir=\$PWD" + gatk --java-options "-Xmx${avail_mem}g -XX:-UsePerfData" \\ GermlineCNVCaller \\ $input_list \\ diff --git a/modules/nf-core/gatk4/intervallisttools/environment.yml b/modules/nf-core/gatk4/intervallisttools/environment.yml index b70e5e14..a4026f98 100644 --- a/modules/nf-core/gatk4/intervallisttools/environment.yml +++ b/modules/nf-core/gatk4/intervallisttools/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/intervallisttools/main.nf b/modules/nf-core/gatk4/intervallisttools/main.nf index 10baa752..400fa038 100644 --- a/modules/nf-core/gatk4/intervallisttools/main.nf +++ b/modules/nf-core/gatk4/intervallisttools/main.nf @@ -4,8 +4,8 @@ process GATK4_INTERVALLISTTOOLS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(intervals) diff --git a/modules/nf-core/gatk4/mergebamalignment/environment.yml b/modules/nf-core/gatk4/mergebamalignment/environment.yml index 08a1df23..0ea7b0d7 100644 --- a/modules/nf-core/gatk4/mergebamalignment/environment.yml +++ b/modules/nf-core/gatk4/mergebamalignment/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/mergebamalignment/main.nf b/modules/nf-core/gatk4/mergebamalignment/main.nf index f970421f..0085026c 100644 --- a/modules/nf-core/gatk4/mergebamalignment/main.nf +++ b/modules/nf-core/gatk4/mergebamalignment/main.nf @@ -4,8 +4,8 @@ process GATK4_MERGEBAMALIGNMENT { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(aligned), path(unmapped) diff --git a/modules/nf-core/gatk4/mergevcfs/environment.yml b/modules/nf-core/gatk4/mergevcfs/environment.yml index d6c3e51a..efd9faa2 100644 --- a/modules/nf-core/gatk4/mergevcfs/environment.yml +++ b/modules/nf-core/gatk4/mergevcfs/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/mergevcfs/main.nf b/modules/nf-core/gatk4/mergevcfs/main.nf index 3362c2bd..9e8d4391 100644 --- a/modules/nf-core/gatk4/mergevcfs/main.nf +++ b/modules/nf-core/gatk4/mergevcfs/main.nf @@ -4,8 +4,8 @@ process GATK4_MERGEVCFS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(vcf) diff --git a/modules/nf-core/gatk4/mergevcfs/meta.yml b/modules/nf-core/gatk4/mergevcfs/meta.yml index 30290a85..996053fc 100644 --- a/modules/nf-core/gatk4/mergevcfs/meta.yml +++ b/modules/nf-core/gatk4/mergevcfs/meta.yml @@ -29,7 +29,7 @@ input: description: | Groovy Map containing reference information e.g. [ id:'genome'] - - ref_dict: + - dict: type: file description: Optional Sequence Dictionary as input pattern: "*.dict" diff --git a/modules/nf-core/gatk4/mergevcfs/tests/main.nf.test b/modules/nf-core/gatk4/mergevcfs/tests/main.nf.test new file mode 100644 index 00000000..77ace10a --- /dev/null +++ b/modules/nf-core/gatk4/mergevcfs/tests/main.nf.test @@ -0,0 +1,87 @@ +nextflow_process { + + name "Test Process GATK4_MERGEVCFS" + script "../main.nf" + process "GATK4_MERGEVCFS" + + tag "modules" + tag "modules_nfcore" + tag "gatk4" + tag "gatk4/mergevcfs" + + test("test_gatk4_mergevcfs") { + when { + process { + """ + input[0] = [ [ id:'test' ], [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz', checkIfExists: true), file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/vcf/gnomAD.r2.1.1.vcf.gz', checkIfExists: true) ]] + input[1] = [ [], file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.dict', checkIfExists: true)] + """ + } + } + + then { + assertAll( + { assert process.success }, + { + assert snapshot( + file(process.out.vcf.get(0).get(1)).name, + file(process.out.tbi.get(0).get(1)).name + ).match("test_gatk4_mergevcfs") + }, + ) + } + + } + + test("test_gatk4_mergevcfs_no_dict") { + when { + process { + """ + input[0] = [ [ id:'test' ], [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz', checkIfExists: true), file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/vcf/gnomAD.r2.1.1.vcf.gz', checkIfExists: true) ]] + input[1] = [ [],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { + assert snapshot( + file(process.out.vcf.get(0).get(1)).name, + file(process.out.tbi.get(0).get(1)).name + ).match("test_gatk4_mergevcfs_no_dict") + }, + ) + } + + } + + test("test_gatk4_mergevcfs_no_dict_stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ [ id:'test' ], [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz', checkIfExists: true), file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/vcf/gnomAD.r2.1.1.vcf.gz', checkIfExists: true) ]] + input[1] = [ [],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { + assert snapshot( + file(process.out.vcf.get(0).get(1)).name, + file(process.out.tbi.get(0).get(1)).name + ).match("test_gatk4_mergevcfs_no_dict_stub") + }, + ) + } + + } + +} diff --git a/modules/nf-core/gatk4/mergevcfs/tests/main.nf.test.snap b/modules/nf-core/gatk4/mergevcfs/tests/main.nf.test.snap new file mode 100644 index 00000000..62cceed5 --- /dev/null +++ b/modules/nf-core/gatk4/mergevcfs/tests/main.nf.test.snap @@ -0,0 +1,35 @@ +{ + "test_gatk4_mergevcfs_no_dict_stub": { + "content": [ + "test.vcf.gz", + "test.vcf.gz.tbi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T14:57:40.784590995" + }, + "test_gatk4_mergevcfs": { + "content": [ + "test.vcf.gz", + "test.vcf.gz.tbi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T14:56:42.178255913" + }, + "test_gatk4_mergevcfs_no_dict": { + "content": [ + "test.vcf.gz", + "test.vcf.gz.tbi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T14:57:11.404322124" + } +} \ No newline at end of file diff --git a/modules/nf-core/gatk4/mergevcfs/tests/tags.yml b/modules/nf-core/gatk4/mergevcfs/tests/tags.yml new file mode 100644 index 00000000..d2a74ba2 --- /dev/null +++ b/modules/nf-core/gatk4/mergevcfs/tests/tags.yml @@ -0,0 +1,2 @@ +gatk4/mergevcfs: + - "modules/nf-core/gatk4/mergevcfs/**" diff --git a/modules/nf-core/gatk4/mutect2/environment.yml b/modules/nf-core/gatk4/mutect2/environment.yml index 54da66ce..86f4bfae 100644 --- a/modules/nf-core/gatk4/mutect2/environment.yml +++ b/modules/nf-core/gatk4/mutect2/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/mutect2/main.nf b/modules/nf-core/gatk4/mutect2/main.nf index 721e94f3..79d8d282 100644 --- a/modules/nf-core/gatk4/mutect2/main.nf +++ b/modules/nf-core/gatk4/mutect2/main.nf @@ -4,8 +4,8 @@ process GATK4_MUTECT2 { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(input), path(input_index), path(intervals) diff --git a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/environment.yml b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/environment.yml deleted file mode 100644 index a515d065..00000000 --- a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/environment.yml +++ /dev/null @@ -1,5 +0,0 @@ -name: gatk4_postprocessgermlinecnvcalls -channels: - - conda-forge - - bioconda - - defaults diff --git a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf index ef9a34a3..31db78bc 100644 --- a/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf +++ b/modules/nf-core/gatk4/postprocessgermlinecnvcalls/main.nf @@ -3,7 +3,7 @@ process GATK4_POSTPROCESSGERMLINECNVCALLS { label 'process_single' //Conda is not supported at the moment: https://github.com/broadinstitute/gatk/issues/7811 - container "nf-core/gatk:4.4.0.0" //Biocontainers is missing a package + container "nf-core/gatk:4.5.0.0" //Biocontainers is missing a package input: tuple val(meta), path(calls), path(model), path(ploidy) @@ -35,6 +35,8 @@ process GATK4_POSTPROCESSGERMLINECNVCALLS { avail_mem = (task.memory.mega*0.8).intValue() } """ + export THEANO_FLAGS="base_compiledir=\$PWD" + gatk --java-options "-Xmx${avail_mem}g -XX:-UsePerfData" \\ PostprocessGermlineCNVCalls \\ $calls_command \\ diff --git a/modules/nf-core/gatk4/preprocessintervals/environment.yml b/modules/nf-core/gatk4/preprocessintervals/environment.yml index 542d9efd..ec0b09e9 100644 --- a/modules/nf-core/gatk4/preprocessintervals/environment.yml +++ b/modules/nf-core/gatk4/preprocessintervals/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/preprocessintervals/main.nf b/modules/nf-core/gatk4/preprocessintervals/main.nf index 61b281da..dffc4bb1 100644 --- a/modules/nf-core/gatk4/preprocessintervals/main.nf +++ b/modules/nf-core/gatk4/preprocessintervals/main.nf @@ -4,8 +4,8 @@ process GATK4_PREPROCESSINTERVALS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/gatk4/printreads/environment.yml b/modules/nf-core/gatk4/printreads/environment.yml index 7387f5d1..d1dd0b3e 100644 --- a/modules/nf-core/gatk4/printreads/environment.yml +++ b/modules/nf-core/gatk4/printreads/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/printreads/main.nf b/modules/nf-core/gatk4/printreads/main.nf index 281096bb..f97180ad 100644 --- a/modules/nf-core/gatk4/printreads/main.nf +++ b/modules/nf-core/gatk4/printreads/main.nf @@ -4,8 +4,8 @@ process GATK4_PRINTREADS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(input), path(index) diff --git a/modules/nf-core/gatk4/revertsam/environment.yml b/modules/nf-core/gatk4/revertsam/environment.yml index 0ce7dddc..f169a89a 100644 --- a/modules/nf-core/gatk4/revertsam/environment.yml +++ b/modules/nf-core/gatk4/revertsam/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/revertsam/main.nf b/modules/nf-core/gatk4/revertsam/main.nf index da3a71f9..635784ab 100644 --- a/modules/nf-core/gatk4/revertsam/main.nf +++ b/modules/nf-core/gatk4/revertsam/main.nf @@ -4,8 +4,8 @@ process GATK4_REVERTSAM { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(bam) diff --git a/modules/nf-core/gatk4/samtofastq/environment.yml b/modules/nf-core/gatk4/samtofastq/environment.yml index 584c3d83..1f881bb7 100644 --- a/modules/nf-core/gatk4/samtofastq/environment.yml +++ b/modules/nf-core/gatk4/samtofastq/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/samtofastq/main.nf b/modules/nf-core/gatk4/samtofastq/main.nf index 02bf2152..35b9b76d 100644 --- a/modules/nf-core/gatk4/samtofastq/main.nf +++ b/modules/nf-core/gatk4/samtofastq/main.nf @@ -4,8 +4,8 @@ process GATK4_SAMTOFASTQ { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(bam) diff --git a/modules/nf-core/gatk4/selectvariants/environment.yml b/modules/nf-core/gatk4/selectvariants/environment.yml index 404bafde..f2711e44 100644 --- a/modules/nf-core/gatk4/selectvariants/environment.yml +++ b/modules/nf-core/gatk4/selectvariants/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/selectvariants/main.nf b/modules/nf-core/gatk4/selectvariants/main.nf index 139d7835..c342ee93 100644 --- a/modules/nf-core/gatk4/selectvariants/main.nf +++ b/modules/nf-core/gatk4/selectvariants/main.nf @@ -4,8 +4,8 @@ process GATK4_SELECTVARIANTS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(vcf), path(vcf_idx), path (intervals) diff --git a/modules/nf-core/gatk4/shiftfasta/environment.yml b/modules/nf-core/gatk4/shiftfasta/environment.yml index 381a22c0..58ebf089 100644 --- a/modules/nf-core/gatk4/shiftfasta/environment.yml +++ b/modules/nf-core/gatk4/shiftfasta/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/shiftfasta/main.nf b/modules/nf-core/gatk4/shiftfasta/main.nf index b0ff3ed0..350ff8d3 100644 --- a/modules/nf-core/gatk4/shiftfasta/main.nf +++ b/modules/nf-core/gatk4/shiftfasta/main.nf @@ -4,8 +4,8 @@ process GATK4_SHIFTFASTA { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/gatk4/splitintervals/environment.yml b/modules/nf-core/gatk4/splitintervals/environment.yml index 6b347417..b2e8c1b0 100644 --- a/modules/nf-core/gatk4/splitintervals/environment.yml +++ b/modules/nf-core/gatk4/splitintervals/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/splitintervals/main.nf b/modules/nf-core/gatk4/splitintervals/main.nf index 957f1675..d59b6afb 100644 --- a/modules/nf-core/gatk4/splitintervals/main.nf +++ b/modules/nf-core/gatk4/splitintervals/main.nf @@ -4,8 +4,8 @@ process GATK4_SPLITINTERVALS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(intervals) diff --git a/modules/nf-core/gatk4/variantfiltration/environment.yml b/modules/nf-core/gatk4/variantfiltration/environment.yml index cc16bc0e..0812b6f2 100644 --- a/modules/nf-core/gatk4/variantfiltration/environment.yml +++ b/modules/nf-core/gatk4/variantfiltration/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gatk4=4.4.0.0 + - bioconda::gatk4=4.5.0.0 diff --git a/modules/nf-core/gatk4/variantfiltration/main.nf b/modules/nf-core/gatk4/variantfiltration/main.nf index 1c4d8ad0..388c60ab 100644 --- a/modules/nf-core/gatk4/variantfiltration/main.nf +++ b/modules/nf-core/gatk4/variantfiltration/main.nf @@ -4,8 +4,8 @@ process GATK4_VARIANTFILTRATION { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0': - 'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0': + 'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }" input: tuple val(meta), path(vcf), path(tbi) diff --git a/modules/nf-core/samtools/faidx/environment.yml b/modules/nf-core/samtools/faidx/environment.yml index 01ccbcc7..3e95dd71 100644 --- a/modules/nf-core/samtools/faidx/environment.yml +++ b/modules/nf-core/samtools/faidx/environment.yml @@ -4,4 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::samtools=1.18 + - bioconda::samtools=1.19.2 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf index d3461627..cfe7ad95 100644 --- a/modules/nf-core/samtools/faidx/main.nf +++ b/modules/nf-core/samtools/faidx/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_FAIDX { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : - 'biocontainers/samtools:1.18--h50ea8bc_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : + 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/samtools/index/environment.yml b/modules/nf-core/samtools/index/environment.yml index 296ed99e..a5e50649 100644 --- a/modules/nf-core/samtools/index/environment.yml +++ b/modules/nf-core/samtools/index/environment.yml @@ -4,4 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::samtools=1.18 + - bioconda::samtools=1.19.2 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf index 8ad18fdc..dc14f98d 100644 --- a/modules/nf-core/samtools/index/main.nf +++ b/modules/nf-core/samtools/index/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_INDEX { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : - 'biocontainers/samtools:1.18--h50ea8bc_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : + 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" input: tuple val(meta), path(input) diff --git a/modules/nf-core/samtools/index/tests/main.nf.test b/modules/nf-core/samtools/index/tests/main.nf.test index c76a9169..bb7756d1 100644 --- a/modules/nf-core/samtools/index/tests/main.nf.test +++ b/modules/nf-core/samtools/index/tests/main.nf.test @@ -8,7 +8,7 @@ nextflow_process { tag "samtools" tag "samtools/index" - test("sarscov2 [BAI]") { + test("bai") { when { params { @@ -16,10 +16,10 @@ nextflow_process { } process { """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) """ } } @@ -28,12 +28,12 @@ nextflow_process { assertAll ( { assert process.success }, { assert snapshot(process.out.bai).match("bai") }, - { assert path(process.out.versions.get(0)).getText().contains("samtools") } + { assert snapshot(process.out.versions).match("bai_versions") } ) } } - test("homo_sapiens [CRAI]") { + test("crai") { when { params { @@ -41,10 +41,10 @@ nextflow_process { } process { """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true) - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true) + ]) """ } } @@ -53,12 +53,12 @@ nextflow_process { assertAll ( { assert process.success }, { assert snapshot(process.out.crai).match("crai") }, - { assert path(process.out.versions.get(0)).getText().contains("samtools") } + { assert snapshot(process.out.versions).match("crai_versions") } ) } } - test("homo_sapiens [CSI]") { + test("csi") { config "./csi.nextflow.config" @@ -68,10 +68,10 @@ nextflow_process { } process { """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) """ } } @@ -80,7 +80,7 @@ nextflow_process { assertAll ( { assert process.success }, { assert path(process.out.csi.get(0).get(1)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("samtools") } + { assert snapshot(process.out.versions).match("csi_versions") } ) } } diff --git a/modules/nf-core/samtools/index/tests/main.nf.test.snap b/modules/nf-core/samtools/index/tests/main.nf.test.snap index b3baee7f..3dc8e7de 100644 --- a/modules/nf-core/samtools/index/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/index/tests/main.nf.test.snap @@ -1,28 +1,74 @@ { + "crai_versions": { + "content": [ + [ + "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:12:00.324667957" + }, + "csi_versions": { + "content": [ + [ + "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:12:07.885103162" + }, "crai": { "content": [ [ [ { - "id": "test" + "id": "test", + "single_end": false }, "test.paired_end.recalibrated.sorted.cram.crai:md5,14bc3bd5c89cacc8f4541f9062429029" ] ] ], - "timestamp": "2023-11-15T15:17:37.30801" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:41:38.446424" }, "bai": { "content": [ [ [ { - "id": "test" + "id": "test", + "single_end": false }, "test.paired_end.sorted.bam.bai:md5,704c10dd1326482448ca3073fdebc2f4" ] ] ], - "timestamp": "2023-11-15T15:17:30.869234" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:40:46.579747" + }, + "bai_versions": { + "content": [ + [ + "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:11:51.641425452" } } \ No newline at end of file diff --git a/modules/nf-core/samtools/merge/environment.yml b/modules/nf-core/samtools/merge/environment.yml index 1f7fe49f..fc669b1b 100644 --- a/modules/nf-core/samtools/merge/environment.yml +++ b/modules/nf-core/samtools/merge/environment.yml @@ -4,4 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::samtools=1.18 + - bioconda::samtools=1.19.2 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/samtools/merge/main.nf b/modules/nf-core/samtools/merge/main.nf index e104b90a..a3048c28 100644 --- a/modules/nf-core/samtools/merge/main.nf +++ b/modules/nf-core/samtools/merge/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_MERGE { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : - 'biocontainers/samtools:1.18--h50ea8bc_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : + 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" input: tuple val(meta), path(input_files, stageAs: "?/*") diff --git a/modules/nf-core/samtools/merge/tests/main.nf.test b/modules/nf-core/samtools/merge/tests/main.nf.test index 024f9f70..40b36e82 100644 --- a/modules/nf-core/samtools/merge/tests/main.nf.test +++ b/modules/nf-core/samtools/merge/tests/main.nf.test @@ -9,21 +9,19 @@ nextflow_process { tag "samtools" tag "samtools/merge" - test("sarscov2 - [bam1, bam2, bam3], [], []") { + test("bams") { config "./index.config" when { process { """ - input[0] = [ - [ id: 'test' ], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_paired_end_methylated_sorted_bam'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_single_end_sorted_bam'], checkIfExists: true) - ] - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.methylated.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam', checkIfExists: true) ] + ]) input[1] = [[],[]] input[2] = [[],[]] """ @@ -33,40 +31,35 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - process.out.cram, - file(process.out.csi[0][1]).name, - process.out.crai, - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("bams_bam") }, + { assert snapshot(process.out.cram).match("bams_cram") }, + { assert snapshot(file(process.out.csi[0][1]).name).match("bams_csi") }, + { assert snapshot(process.out.crai).match("bams_crai") }, + { assert snapshot(process.out.versions).match("bams_versions") } ) } - } - test("homo_sapiens - [cram1, cram2], fasta, fai") { + test("crams") { config "./index.config" when { process { """ - input[0] = [ - [ id: 'test' ], // meta map - [ - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true), - file(params.test_data['homo_sapiens']['illumina']['test2_paired_end_recalibrated_sorted_cram'], checkIfExists: true), - ] - ] - input[1] = [ - [id:'genome'], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - input[2] = [ - [id:'genome'], - file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test2.paired_end.recalibrated.sorted.cram', checkIfExists: true) ] + ]) + input[1] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) + ]) """ } } @@ -74,27 +67,24 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.cram[0][1]).name, - process.out.bam, - file(process.out.crai[0][1]).name, - process.out.csi, - process.out.versions - ).match() } + { assert snapshot(file(process.out.cram[0][1]).name).match("crams_cram") }, + { assert snapshot(process.out.bam).match("crams_bam") }, + { assert snapshot(file(process.out.crai[0][1]).name).match("crams_crai") }, + { assert snapshot(process.out.csi).match("crams_csi") }, + { assert snapshot(process.out.versions).match("crams_versions") } ) } - } - test("sarscov2 - bam, [], []") { + test("bam") { when { process { """ - input[0] = [ - [ id: 'test' ], // meta map - file(params.test_data['sarscov2']['illumina']['test_paired_end_methylated_sorted_bam'], checkIfExists: true), - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.methylated.sorted.bam', checkIfExists: true) ] + ]) input[1] = [[],[]] input[2] = [[],[]] """ @@ -104,19 +94,16 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - process.out.cram, - process.out.crai, - process.out.csi, - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("bam_bam") }, + { assert snapshot(process.out.cram).match("bam_cram") }, + { assert snapshot(process.out.crai).match("bam_crai") }, + { assert snapshot(process.out.csi).match("bam_csi") }, + { assert snapshot(process.out.versions).match("bam_versions") } ) } - } - test("sarscov2 - [bam1, bam2, bam3], [], [] - stub") { + test("bams_stub") { config "./index.config" options "-stub" @@ -124,14 +111,12 @@ nextflow_process { when { process { """ - input[0] = [ - [ id: 'test' ], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_paired_end_methylated_sorted_bam'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_single_end_sorted_bam'], checkIfExists: true) - ] - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.methylated.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam', checkIfExists: true) ] + ]) input[1] = [[],[]] input[2] = [[],[]] """ @@ -141,16 +126,12 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - process.out.cram, - file(process.out.csi[0][1]).name, - process.out.crai, - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("bams_stub_bam") }, + { assert snapshot(process.out.cram).match("bams_stub_cram") }, + { assert snapshot(file(process.out.csi[0][1]).name).match("bams_stub_csi") }, + { assert snapshot(process.out.crai).match("bams_stub_crai") }, + { assert snapshot(process.out.versions).match("bams_stub_versions") } ) } - } - } diff --git a/modules/nf-core/samtools/merge/tests/main.nf.test.snap b/modules/nf-core/samtools/merge/tests/main.nf.test.snap index 3ab57d81..f7da7699 100644 --- a/modules/nf-core/samtools/merge/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/merge/tests/main.nf.test.snap @@ -1,68 +1,228 @@ { - "sarscov2 - bam, [], []": { + "crams_cram": { + "content": [ + "test.cram" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:00.647389" + }, + "bams_stub_cram": { "content": [ - "test.bam", [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:19.937013" + }, + "bams_crai": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:49:24.928616" + }, + "bams_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:49:24.923289" + }, + "bams_cram": { + "content": [ [ - ], - [ - "versions.yml:md5,71986103374bdddb2e3093d20e7d06cb" ] ], - "timestamp": "2023-12-04T17:13:30.244841621" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:49:24.925716" }, - "sarscov2 - [bam1, bam2, bam3], [], [] - stub": { + "crams_csi": { "content": [ - "test.bam", [ - ], - "test.csi", + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:00.655959" + }, + "bam_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:10.319539" + }, + "bam_versions": { + "content": [ + [ + "versions.yml:md5,52c62d4712f7af00eb962d090ca32fe4" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:16:33.782637377" + }, + "bams_csi": { + "content": [ + "test.bam.csi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:49:24.92719" + }, + "bams_stub_csi": { + "content": [ + "test.csi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:19.940498" + }, + "bam_crai": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:10.328852" + }, + "bams_stub_versions": { + "content": [ [ - "versions.yml:md5,71986103374bdddb2e3093d20e7d06cb" + "versions.yml:md5,52c62d4712f7af00eb962d090ca32fe4" ] ], - "timestamp": "2023-12-04T17:10:14.861445721" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:16:42.594476052" }, - "homo_sapiens - [cram1, cram2], fasta, fai": { + "bam_cram": { "content": [ - "test.cram", [ - ], - "test.cram.crai", + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:10.324219" + }, + "bams_stub_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:19.933153" + }, + "bams_versions": { + "content": [ + [ + "versions.yml:md5,52c62d4712f7af00eb962d090ca32fe4" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:16:04.805335656" + }, + "crams_bam": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:00.650652" + }, + "crams_versions": { + "content": [ [ - "versions.yml:md5,71986103374bdddb2e3093d20e7d06cb" + "versions.yml:md5,52c62d4712f7af00eb962d090ca32fe4" ] ], - "timestamp": "2023-12-04T17:09:29.716002618" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:16:25.889394689" }, - "sarscov2 - [bam1, bam2, bam3], [], []": { + "bam_csi": { "content": [ - "test.bam", [ - ], - "test.bam.csi", + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:10.33292" + }, + "crams_crai": { + "content": [ + "test.cram.crai" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:00.653512" + }, + "bams_stub_crai": { + "content": [ [ - ], - [ - "versions.yml:md5,71986103374bdddb2e3093d20e7d06cb" ] ], - "timestamp": "2023-12-04T17:08:42.329973045" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:50:19.943839" } } \ No newline at end of file diff --git a/modules/nf-core/samtools/sort/environment.yml b/modules/nf-core/samtools/sort/environment.yml index cd50868c..4d898e48 100644 --- a/modules/nf-core/samtools/sort/environment.yml +++ b/modules/nf-core/samtools/sort/environment.yml @@ -4,4 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::samtools=1.18 + - bioconda::samtools=1.19.2 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf index 4a666d42..cdd8305d 100644 --- a/modules/nf-core/samtools/sort/main.nf +++ b/modules/nf-core/samtools/sort/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_SORT { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : - 'biocontainers/samtools:1.18--h50ea8bc_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : + 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" input: tuple val(meta), path(bam) diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test b/modules/nf-core/samtools/sort/tests/main.nf.test index abb80978..31e24b88 100644 --- a/modules/nf-core/samtools/sort/tests/main.nf.test +++ b/modules/nf-core/samtools/sort/tests/main.nf.test @@ -8,7 +8,7 @@ nextflow_process { tag "samtools" tag "samtools/sort" - test("test_samtools_sort") { + test("bam") { config "./nextflow.config" @@ -18,12 +18,10 @@ nextflow_process { } process { """ - input[0] = [ - [ id:'test', single_end:false ], - [ - file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true) - ] - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true) + ]) """ } } @@ -34,10 +32,9 @@ nextflow_process { { assert snapshot(process.out).match() } ) } - } - test("test_samtools_sort_stub") { + test("bam_stub") { config "./nextflow.config" options "-stub-run" @@ -48,12 +45,10 @@ nextflow_process { } process { """ - input[0] = [ - [ id:'test', single_end:false ], - [ - file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true) - ] - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true) + ]) """ } } @@ -61,13 +56,9 @@ nextflow_process { then { assertAll ( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("bam_stub_bam") }, + { assert snapshot(process.out.versions).match("bam_stub_versions") } ) } - } - } diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test.snap b/modules/nf-core/samtools/sort/tests/main.nf.test.snap index ff722259..a7cf0210 100644 --- a/modules/nf-core/samtools/sort/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/sort/tests/main.nf.test.snap @@ -1,5 +1,27 @@ { - "test_samtools_sort": { + "bam_stub_bam": { + "content": [ + "test.sorted.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:21:04.364044" + }, + "bam_stub_versions": { + "content": [ + [ + "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:15:00.20800281" + }, + "bam": { "content": [ { "0": [ @@ -8,14 +30,14 @@ "id": "test", "single_end": false }, - "test.sorted.bam:md5,ea6a0fef94eb534e901f107a05a33a06" + "test.sorted.bam:md5,c6ea1346ec4aae007eb40b708935088c" ] ], "1": [ ], "2": [ - "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80" + "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca" ], "bam": [ [ @@ -23,26 +45,21 @@ "id": "test", "single_end": false }, - "test.sorted.bam:md5,ea6a0fef94eb534e901f107a05a33a06" + "test.sorted.bam:md5,c6ea1346ec4aae007eb40b708935088c" ] ], "csi": [ ], "versions": [ - "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80" + "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca" ] } ], - "timestamp": "2023-12-04T11:11:22.005628301" - }, - "test_samtools_sort_stub": { - "content": [ - "test.sorted.bam", - [ - "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80" - ] - ], - "timestamp": "2023-12-04T17:47:22.314445935" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:14:52.736359271" } } \ No newline at end of file diff --git a/modules/nf-core/samtools/stats/environment.yml b/modules/nf-core/samtools/stats/environment.yml index b45ba90c..67bb0ca4 100644 --- a/modules/nf-core/samtools/stats/environment.yml +++ b/modules/nf-core/samtools/stats/environment.yml @@ -4,5 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::samtools=1.18 - - bioconda::htslib=1.18 + - bioconda::samtools=1.19.2 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf index 7539140a..52b00f4b 100644 --- a/modules/nf-core/samtools/stats/main.nf +++ b/modules/nf-core/samtools/stats/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_STATS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : - 'biocontainers/samtools:1.18--h50ea8bc_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : + 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" input: tuple val(meta), path(input), path(input_index) diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test b/modules/nf-core/samtools/stats/tests/main.nf.test index 20c3efe1..e3d5cb14 100644 --- a/modules/nf-core/samtools/stats/tests/main.nf.test +++ b/modules/nf-core/samtools/stats/tests/main.nf.test @@ -8,71 +8,58 @@ nextflow_process { tag "samtools" tag "samtools/stats" - test("SAMTOOLS STATS Should run without failures") { + test("bam") { when { params { - outdir = "$outputDir" } process { """ - // define inputs of the process here. - input[0] = [ - [ id:'test', single_end:false ], // meta map - file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true) - - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true) + ]) input[1] = [[],[]] """ - } } then { assertAll( - {assert process.success}, - {assert snapshot(process.out).match()} + {assert process.success}, + {assert snapshot(process.out).match()} ) } - } - test("SAMTOOLS CRAM Should run without failures") { + test("cram") { when { params { - outdir = "$outputDir" } process { """ - // define inputs of the process here - input[0] = [ - [ id:'test', single_end:false ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true), - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram_crai'], checkIfExists: true) - - ] - input[1] = [ - [ id:'genome' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) """ } - - } then { assertAll( - {assert process.success}, - {assert snapshot(process.out).match()} + {assert process.success}, + {assert snapshot(process.out).match()} ) } - } - - } diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test.snap b/modules/nf-core/samtools/stats/tests/main.nf.test.snap index 025c83a5..1b7c9ba4 100644 --- a/modules/nf-core/samtools/stats/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/stats/tests/main.nf.test.snap @@ -1,5 +1,5 @@ { - "SAMTOOLS STATS Should run without failures": { + "cram": { "content": [ { "0": [ @@ -8,11 +8,11 @@ "id": "test", "single_end": false }, - "test.stats:md5,045a48208b1c6f5b8af4347fe31f4def" + "test.stats:md5,01812900aa4027532906c5d431114233" ] ], "1": [ - "versions.yml:md5,650a365c6635001436008350ae83337c" + "versions.yml:md5,0514ceb1769b2a88843e08c1f82624a9" ], "stats": [ [ @@ -20,17 +20,21 @@ "id": "test", "single_end": false }, - "test.stats:md5,045a48208b1c6f5b8af4347fe31f4def" + "test.stats:md5,01812900aa4027532906c5d431114233" ] ], "versions": [ - "versions.yml:md5,650a365c6635001436008350ae83337c" + "versions.yml:md5,0514ceb1769b2a88843e08c1f82624a9" ] } ], - "timestamp": "2023-12-04T11:07:28.26821485" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:15:25.562429714" }, - "SAMTOOLS CRAM Should run without failures": { + "bam": { "content": [ { "0": [ @@ -39,11 +43,11 @@ "id": "test", "single_end": false }, - "test.stats:md5,dfbfa130d4a6925ddd1931dcd8354a43" + "test.stats:md5,5d8681bf541199898c042bf400391d59" ] ], "1": [ - "versions.yml:md5,650a365c6635001436008350ae83337c" + "versions.yml:md5,0514ceb1769b2a88843e08c1f82624a9" ], "stats": [ [ @@ -51,14 +55,18 @@ "id": "test", "single_end": false }, - "test.stats:md5,dfbfa130d4a6925ddd1931dcd8354a43" + "test.stats:md5,5d8681bf541199898c042bf400391d59" ] ], "versions": [ - "versions.yml:md5,650a365c6635001436008350ae83337c" + "versions.yml:md5,0514ceb1769b2a88843e08c1f82624a9" ] } ], - "timestamp": "2023-12-04T11:07:50.356233402" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:15:07.857611509" } } \ No newline at end of file diff --git a/modules/nf-core/samtools/view/environment.yml b/modules/nf-core/samtools/view/environment.yml index 99aa69d0..b0676f33 100644 --- a/modules/nf-core/samtools/view/environment.yml +++ b/modules/nf-core/samtools/view/environment.yml @@ -4,4 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::samtools=1.18 + - bioconda::samtools=1.19.2 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf index 0b5a2912..5a8989d6 100644 --- a/modules/nf-core/samtools/view/main.nf +++ b/modules/nf-core/samtools/view/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_VIEW { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : - 'biocontainers/samtools:1.18--h50ea8bc_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : + 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" input: tuple val(meta), path(input), path(index) diff --git a/modules/nf-core/samtools/view/tests/main.nf.test b/modules/nf-core/samtools/view/tests/main.nf.test index 89ed3555..45a0defb 100644 --- a/modules/nf-core/samtools/view/tests/main.nf.test +++ b/modules/nf-core/samtools/view/tests/main.nf.test @@ -9,16 +9,16 @@ nextflow_process { tag "samtools" tag "samtools/view" - test("sarscov2 - [bam, []], [], []") { + test("bam") { when { process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true), [] - ] + ]) input[1] = [[],[]] input[2] = [] """ @@ -28,34 +28,31 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - process.out.cram, - process.out.sam, - process.out.bai, - process.out.crai, - process.out.csi, - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("bam_bam") }, + { assert snapshot(process.out.bai).match("bam_bai") }, + { assert snapshot(process.out.crai).match("bam_crai") }, + { assert snapshot(process.out.cram).match("bam_cram") }, + { assert snapshot(process.out.csi).match("bam_csi") }, + { assert snapshot(process.out.sam).match("bam_sam") }, + { assert snapshot(process.out.versions).match("bam_versions") } ) } - } - test("homo_sapiens - [cram, crai], fasta, []") { + test("cram") { when { process { """ - input[0] = [ - [ id: 'test' ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true), - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram_crai'], checkIfExists: true) - ] - input[1] = [ - [ id:'genome' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) input[2] = [] """ } @@ -64,36 +61,33 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.cram[0][1]).name, - process.out.bam, - process.out.sam, - process.out.bai, - process.out.crai, - process.out.csi, - process.out.versions - ).match() } + { assert snapshot(file(process.out.cram[0][1]).name).match("cram_cram") }, + { assert snapshot(process.out.bai).match("cram_bai") }, + { assert snapshot(process.out.bam).match("cram_bam") }, + { assert snapshot(process.out.crai).match("cram_crai") }, + { assert snapshot(process.out.csi).match("cram_csi") }, + { assert snapshot(process.out.sam).match("cram_sam") }, + { assert snapshot(process.out.versions).match("cram_versions") } ) } - } - test("homo_sapiens - [cram, []], fasta, [] - bam output") { + test("cram_to_bam") { config "./bam.config" when { process { """ - input[0] = [ - [ id: 'test' ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true), + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true), [] - ] - input[1] = [ - [ id:'genome' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] + ]) + input[1] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) input[2] = [] """ } @@ -102,36 +96,33 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - process.out.cram, - process.out.sam, - process.out.bai, - process.out.crai, - process.out.csi, - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_bam") }, + { assert snapshot(process.out.bai).match("cram_to_bam_bai") }, + { assert snapshot(process.out.crai).match("cram_to_bam_crai") }, + { assert snapshot(process.out.cram).match("cram_to_bam_cram") }, + { assert snapshot(process.out.csi).match("cram_to_bam_csi") }, + { assert snapshot(process.out.sam).match("cram_to_bam_sam") }, + { assert snapshot(process.out.versions).match("cram_to_bam_versions") } ) } - } - test("homo_sapiens - [cram, []], fasta, [] - bam & index output") { + test("cram_to_bam_index") { config "./bam_index.config" when { process { """ - input[0] = [ - [ id: 'test' ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true), + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true), [] - ] - input[1] = [ - [ id:'genome' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] + ]) + input[1] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) input[2] = [] """ } @@ -140,36 +131,33 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - process.out.cram, - process.out.sam, - file(process.out.csi[0][1]).name, - process.out.crai, - process.out.bai, - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_index_bam") }, + { assert snapshot(file(process.out.csi[0][1]).name).match("cram_to_bam_index_csi") }, + { assert snapshot(process.out.bai).match("cram_to_bam_index_bai") }, + { assert snapshot(process.out.crai).match("cram_to_bam_index_crai") }, + { assert snapshot(process.out.cram).match("cram_to_bam_index_cram") }, + { assert snapshot(process.out.sam).match("cram_to_bam_index_sam") }, + { assert snapshot(process.out.versions).match("cram_to_bam_index_versions") } ) } - } - test("homo_sapiens - [cram, []], fasta, qname - bam & index output") { + test("cram_to_bam_index_qname") { config "./bam_index.config" when { process { """ - input[0] = [ - [ id: 'test' ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true), + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true), [] - ] - input[1] = [ - [ id:'genome' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] + ]) + input[1] = Channel.of([ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) input[2] = Channel.of("testN:2817", "testN:2814").collectFile(name: "readnames.list", newLine: true) """ } @@ -178,21 +166,18 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - process.out.cram, - process.out.sam, - file(process.out.csi[0][1]).name, - process.out.crai, - process.out.bai, - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_index_qname_bam") }, + { assert snapshot(file(process.out.csi[0][1]).name).match("cram_to_bam_index_qname_csi") }, + { assert snapshot(process.out.bai).match("cram_to_bam_index_qname_bai") }, + { assert snapshot(process.out.crai).match("cram_to_bam_index_qname_crai") }, + { assert snapshot(process.out.cram).match("cram_to_bam_index_qname_cram") }, + { assert snapshot(process.out.sam).match("cram_to_bam_index_qname_sam") }, + { assert snapshot(process.out.versions).match("cram_to_bam_index_qname_versions") } ) } - } - test("sarscov2 - [bam, []], [], [] - stub") { + test("bam_stub") { options "-stub" config "./bam_index.config" @@ -200,11 +185,11 @@ nextflow_process { when { process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true), [] - ] + ]) input[1] = [[],[]] input[2] = [] """ @@ -214,18 +199,14 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - process.out.cram, - process.out.sam, - file(process.out.csi[0][1]).name, - process.out.crai, - process.out.bai, - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("bam_stub_bam") }, + { assert snapshot(file(process.out.csi[0][1]).name).match("bam_stub_csi") }, + { assert snapshot(process.out.bai).match("bam_stub_bai") }, + { assert snapshot(process.out.crai).match("bam_stub_crai") }, + { assert snapshot(process.out.cram).match("bam_stub_cram") }, + { assert snapshot(process.out.sam).match("bam_stub_sam") }, + { assert snapshot(process.out.versions).match("bam_stub_versions") } ) } - } - } diff --git a/modules/nf-core/samtools/view/tests/main.nf.test.snap b/modules/nf-core/samtools/view/tests/main.nf.test.snap index 83427491..f55943a7 100644 --- a/modules/nf-core/samtools/view/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/view/tests/main.nf.test.snap @@ -1,140 +1,488 @@ { - "homo_sapiens - [cram, []], fasta, [] - bam output": { + "bam_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:51.256068" + }, + "cram_to_bam_index_csi": { + "content": [ + "test.bam.csi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:12.958617" + }, + "bam_stub_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:32.065301" + }, + "bam_bai": { "content": [ - "test.bam", [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:51.258578" + }, + "bam_stub_bai": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:32.071284" + }, + "bam_stub_versions": { + "content": [ [ - - ], + "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:13:09.713353823" + }, + "cram_to_bam_index_cram": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:12.972288" + }, + "cram_to_bam_sam": { + "content": [ [ - ], - [ - "versions.yml:md5,06b9049228b111e7bed5c52fe8a98d9b" ] ], - "timestamp": "2023-12-04T17:41:17.563069206" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:04.999247" }, - "sarscov2 - [bam, []], [], []": { + "cram_to_bam_index_sam": { "content": [ - "test.bam", [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:12.976457" + }, + "cram_crai": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:56.497581" + }, + "cram_csi": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:56.50038" + }, + "cram_to_bam_cram": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:04.992239" + }, + "cram_to_bam_index_qname_csi": { + "content": [ + "test.bam.csi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.325496" + }, + "bam_stub_sam": { + "content": [ [ - ], - [ - "versions.yml:md5,06b9049228b111e7bed5c52fe8a98d9b" ] ], - "timestamp": "2023-12-04T17:41:03.206994564" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:32.079529" }, - "homo_sapiens - [cram, []], fasta, qname - bam & index output": { + "cram_cram": { + "content": [ + "test.cram" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:56.490286" + }, + "bam_csi": { "content": [ - "test.bam", [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:51.262882" + }, + "cram_to_bam_crai": { + "content": [ [ - ], - "test.bam.csi", + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:04.989247" + }, + "cram_to_bam_index_crai": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:12.967681" + }, + "cram_to_bam_index_qname_versions": { + "content": [ [ - - ], + "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:13:03.935041046" + }, + "cram_to_bam_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:04.982361" + }, + "cram_to_bam_index_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:12.95456" + }, + "cram_to_bam_index_versions": { + "content": [ [ - "versions.yml:md5,06b9049228b111e7bed5c52fe8a98d9b" + "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1" ] ], - "timestamp": "2023-12-04T17:44:39.165289759" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:12:55.910685496" }, - "homo_sapiens - [cram, []], fasta, [] - bam & index output": { + "cram_to_bam_bai": { "content": [ - "test.bam", [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:04.98601" + }, + "cram_to_bam_versions": { + "content": [ [ - - ], - "test.bam.csi", + "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:12:47.715221169" + }, + "cram_bam": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:56.495512" + }, + "bam_stub_cram": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:32.076908" + }, + "cram_to_bam_index_qname_bai": { + "content": [ [ - "versions.yml:md5,06b9049228b111e7bed5c52fe8a98d9b" + ] ], - "timestamp": "2023-12-04T17:44:32.25731224" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.328458" }, - "sarscov2 - [bam, []], [], [] - stub": { + "cram_to_bam_index_qname_crai": { "content": [ - "test.bam", [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.330789" + }, + "cram_bai": { + "content": [ [ - ], - "test.csi", + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:56.493129" + }, + "bam_stub_crai": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:32.074313" + }, + "cram_to_bam_index_qname_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.322874" + }, + "bam_versions": { + "content": [ + [ + "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:12:31.692607421" + }, + "cram_to_bam_index_qname_cram": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.333248" + }, + "bam_crai": { + "content": [ [ - "versions.yml:md5,06b9049228b111e7bed5c52fe8a98d9b" + ] ], - "timestamp": "2023-12-04T17:44:45.81037195" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:51.259774" }, - "homo_sapiens - [cram, crai], fasta, []": { + "bam_cram": { "content": [ - "test.cram", [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:51.261287" + }, + "cram_to_bam_csi": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:04.995454" + }, + "cram_sam": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:56.502625" + }, + "cram_versions": { + "content": [ + [ + "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:12:39.913411036" + }, + "bam_sam": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:37:51.264651" + }, + "cram_to_bam_index_bai": { + "content": [ [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:12.962863" + }, + "cram_to_bam_index_qname_sam": { + "content": [ [ - "versions.yml:md5,06b9049228b111e7bed5c52fe8a98d9b" + ] ], - "timestamp": "2023-12-04T17:41:10.730011823" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:23.337634" + }, + "bam_stub_csi": { + "content": [ + "test.csi" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T19:38:32.068596" } } \ No newline at end of file diff --git a/modules/nf-core/sentieon/bwaindex/main.nf b/modules/nf-core/sentieon/bwaindex/main.nf index 0d99cb67..e36dcc34 100644 --- a/modules/nf-core/sentieon/bwaindex/main.nf +++ b/modules/nf-core/sentieon/bwaindex/main.nf @@ -22,7 +22,7 @@ process SENTIEON_BWAINDEX { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' @@ -52,7 +52,7 @@ process SENTIEON_BWAINDEX { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' diff --git a/modules/nf-core/sentieon/bwamem/main.nf b/modules/nf-core/sentieon/bwamem/main.nf index e25515e7..230297d0 100644 --- a/modules/nf-core/sentieon/bwamem/main.nf +++ b/modules/nf-core/sentieon/bwamem/main.nf @@ -27,7 +27,7 @@ process SENTIEON_BWAMEM { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' @@ -77,7 +77,7 @@ process SENTIEON_BWAMEM { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' diff --git a/modules/nf-core/sentieon/datametrics/main.nf b/modules/nf-core/sentieon/datametrics/main.nf index 3be2d11a..d1678187 100644 --- a/modules/nf-core/sentieon/datametrics/main.nf +++ b/modules/nf-core/sentieon/datametrics/main.nf @@ -31,7 +31,7 @@ process SENTIEON_DATAMETRICS { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' @@ -83,7 +83,7 @@ process SENTIEON_DATAMETRICS { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' diff --git a/modules/nf-core/sentieon/dedup/main.nf b/modules/nf-core/sentieon/dedup/main.nf index 316993cc..fbcd595f 100644 --- a/modules/nf-core/sentieon/dedup/main.nf +++ b/modules/nf-core/sentieon/dedup/main.nf @@ -32,7 +32,7 @@ process SENTIEON_DEDUP { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' @@ -84,7 +84,7 @@ process SENTIEON_DEDUP { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' diff --git a/modules/nf-core/sentieon/dnamodelapply/main.nf b/modules/nf-core/sentieon/dnamodelapply/main.nf index 1cbb02e3..b728a5ec 100644 --- a/modules/nf-core/sentieon/dnamodelapply/main.nf +++ b/modules/nf-core/sentieon/dnamodelapply/main.nf @@ -28,7 +28,7 @@ process SENTIEON_DNAMODELAPPLY { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' @@ -77,7 +77,7 @@ process SENTIEON_DNAMODELAPPLY { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' diff --git a/modules/nf-core/sentieon/dnascope/main.nf b/modules/nf-core/sentieon/dnascope/main.nf index 7e5adefc..6adea35e 100644 --- a/modules/nf-core/sentieon/dnascope/main.nf +++ b/modules/nf-core/sentieon/dnascope/main.nf @@ -35,7 +35,7 @@ process SENTIEON_DNASCOPE { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' @@ -94,7 +94,7 @@ process SENTIEON_DNASCOPE { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' diff --git a/modules/nf-core/sentieon/readwriter/main.nf b/modules/nf-core/sentieon/readwriter/main.nf index b9afe576..0bace538 100644 --- a/modules/nf-core/sentieon/readwriter/main.nf +++ b/modules/nf-core/sentieon/readwriter/main.nf @@ -28,7 +28,7 @@ process SENTIEON_READWRITER { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' @@ -82,7 +82,7 @@ process SENTIEON_READWRITER { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' diff --git a/modules/nf-core/sentieon/wgsmetrics/main.nf b/modules/nf-core/sentieon/wgsmetrics/main.nf index b16641d9..a028e4a1 100644 --- a/modules/nf-core/sentieon/wgsmetrics/main.nf +++ b/modules/nf-core/sentieon/wgsmetrics/main.nf @@ -28,7 +28,7 @@ process SENTIEON_WGSMETRICS { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' @@ -78,7 +78,7 @@ process SENTIEON_WGSMETRICS { // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity. // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers. // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error. - if (workflow.containerEngine == 'singularity') { + if (workflow.containerEngine in ['singularity','apptainer']) { fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH' } else { fix_ld_library_path = '' diff --git a/modules/nf-core/svdb/merge/environment.yml b/modules/nf-core/svdb/merge/environment.yml index 4aad50da..e6fec088 100644 --- a/modules/nf-core/svdb/merge/environment.yml +++ b/modules/nf-core/svdb/merge/environment.yml @@ -6,4 +6,5 @@ channels: dependencies: - svdb=2.8.1 # renovate: datasource=conda depName=bioconda/samtools - - samtools=1.16.1 + - samtools=1.19.2 + - htslib=1.19.1 diff --git a/modules/nf-core/svdb/merge/main.nf b/modules/nf-core/svdb/merge/main.nf index 827d11b3..c24a9a7c 100644 --- a/modules/nf-core/svdb/merge/main.nf +++ b/modules/nf-core/svdb/merge/main.nf @@ -3,8 +3,8 @@ process SVDB_MERGE { label 'process_medium' conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-c8daa8f9d69d3c5a1a4ff08283a166c18edb0000:af6f8534cd538a85ff43a2eae1b52b143e7abd05-0': - 'biocontainers/mulled-v2-c8daa8f9d69d3c5a1a4ff08283a166c18edb0000:af6f8534cd538a85ff43a2eae1b52b143e7abd05-0' }" + 'https://depot.galaxyproject.org/singularity/mulled-v2-c8daa8f9d69d3c5a1a4ff08283a166c18edb0000:511069f65a53621c5503e5cfee319aa3c735abfa-0': + 'biocontainers/mulled-v2-c8daa8f9d69d3c5a1a4ff08283a166c18edb0000:511069f65a53621c5503e5cfee319aa3c735abfa-0' }" input: tuple val(meta), path(vcfs) diff --git a/modules/nf-core/svdb/merge/meta.yml b/modules/nf-core/svdb/merge/meta.yml index 84265acb..e53e61fe 100644 --- a/modules/nf-core/svdb/merge/meta.yml +++ b/modules/nf-core/svdb/merge/meta.yml @@ -36,7 +36,7 @@ output: - vcf: type: file description: merged VCF file - pattern: "*_sv_merge.vcf.gz" + pattern: "*.vcf.gz" authors: - "@ramprasadn" maintainers: diff --git a/modules/nf-core/svdb/merge/tests/main.nf.test b/modules/nf-core/svdb/merge/tests/main.nf.test index f06b5998..42f7c570 100644 --- a/modules/nf-core/svdb/merge/tests/main.nf.test +++ b/modules/nf-core/svdb/merge/tests/main.nf.test @@ -13,10 +13,11 @@ nextflow_process { when { process { """ - input[0] = [[ id:'test' ], // meta map - [file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_vcf'], checkIfExists: true) ] - ] + input[0] = Channel.of([ + [ id:'test' ], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test2.vcf', checkIfExists: true) ] + ]) input[1] = [ 'tiddit', 'cnvnator'] """ } @@ -36,10 +37,11 @@ nextflow_process { when { process { """ - input[0] = [[ id:'test' ], // meta map - [file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_vcf'], checkIfExists: true) ] - ] + input[0] = Channel.of([ + [ id:'test' ], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test2.vcf', checkIfExists: true) ] + ]) input[1] = [] """ } diff --git a/modules/nf-core/untar/tests/main.nf.test b/modules/nf-core/untar/tests/main.nf.test index 679e83c7..2a7c97bf 100644 --- a/modules/nf-core/untar/tests/main.nf.test +++ b/modules/nf-core/untar/tests/main.nf.test @@ -3,17 +3,12 @@ nextflow_process { name "Test Process UNTAR" script "../main.nf" process "UNTAR" - tag "modules" tag "modules_nfcore" tag "untar" - test("test_untar") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = [ [], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/db/kraken2.tar.gz', checkIfExists: true) ] @@ -33,9 +28,6 @@ nextflow_process { test("test_untar_onlyfiles") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = [ [], file(params.modules_testdata_base_path + 'generic/tar/hello.tar.gz', checkIfExists: true) ] diff --git a/modules/nf-core/untar/tests/main.nf.test.snap b/modules/nf-core/untar/tests/main.nf.test.snap index ace42576..64550292 100644 --- a/modules/nf-core/untar/tests/main.nf.test.snap +++ b/modules/nf-core/untar/tests/main.nf.test.snap @@ -12,7 +12,11 @@ ] ] ], - "timestamp": "2023-10-18T11:56:46.878844" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T11:49:41.320643" }, "test_untar": { "content": [ @@ -29,6 +33,10 @@ ] ] ], - "timestamp": "2023-10-18T11:56:08.16574" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T11:49:33.795172" } } \ No newline at end of file From d0b184f0f7a162e5276f4dd01878e0101ae22749 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 4 Mar 2024 12:49:09 +0100 Subject: [PATCH 361/387] address warnings --- modules.json | 6 +- modules/local/mt_deletion_script.nf | 6 +- modules/nf-core/tabix/bgzip/environment.yml | 1 + modules/nf-core/tabix/bgzip/main.nf | 7 +- .../tabix/bgzip/tests/bgzip_compress.config | 5 + .../nf-core/tabix/bgzip/tests/main.nf.test | 111 +++++++++ .../tabix/bgzip/tests/main.nf.test.snap | 186 +++++++++++++++ modules/nf-core/tabix/bgzip/tests/tags.yml | 2 + .../nf-core/tabix/bgzip/tests/vcf_none.config | 5 + .../nf-core/tabix/bgziptabix/environment.yml | 1 + modules/nf-core/tabix/bgziptabix/main.nf | 6 +- .../tabix/bgziptabix/tests/main.nf.test | 94 ++++++++ .../tabix/bgziptabix/tests/main.nf.test.snap | 143 ++++++++++++ .../tabix/bgziptabix/tests/tabix_csi.config | 5 + .../tabix/bgziptabix/tests/tabix_tbi.config | 5 + .../nf-core/tabix/bgziptabix/tests/tags.yml | 2 + modules/nf-core/tabix/tabix/environment.yml | 1 + modules/nf-core/tabix/tabix/main.nf | 7 +- .../nf-core/tabix/tabix/tests/main.nf.test | 142 ++++++++++++ .../tabix/tabix/tests/main.nf.test.snap | 217 ++++++++++++++++++ .../tabix/tabix/tests/tabix_bed.config | 5 + .../tabix/tabix/tests/tabix_gff.config | 5 + .../tabix/tabix/tests/tabix_vcf_csi.config | 5 + .../tabix/tabix/tests/tabix_vcf_tbi.config | 5 + modules/nf-core/tabix/tabix/tests/tags.yml | 2 + .../local/annotate_mobile_elements.nf | 1 - subworkflows/local/annotate_mt_snvs.nf | 1 + subworkflows/local/call_repeat_expansions.nf | 1 - .../local/call_structural_variants.nf | 3 +- subworkflows/local/qc_bam.nf | 1 + 30 files changed, 963 insertions(+), 18 deletions(-) create mode 100644 modules/nf-core/tabix/bgzip/tests/bgzip_compress.config create mode 100644 modules/nf-core/tabix/bgzip/tests/main.nf.test create mode 100644 modules/nf-core/tabix/bgzip/tests/main.nf.test.snap create mode 100644 modules/nf-core/tabix/bgzip/tests/tags.yml create mode 100644 modules/nf-core/tabix/bgzip/tests/vcf_none.config create mode 100644 modules/nf-core/tabix/bgziptabix/tests/main.nf.test create mode 100644 modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap create mode 100644 modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config create mode 100644 modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config create mode 100644 modules/nf-core/tabix/bgziptabix/tests/tags.yml create mode 100644 modules/nf-core/tabix/tabix/tests/main.nf.test create mode 100644 modules/nf-core/tabix/tabix/tests/main.nf.test.snap create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_bed.config create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_gff.config create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config create mode 100644 modules/nf-core/tabix/tabix/tests/tags.yml diff --git a/modules.json b/modules.json index 54d8ed01..b863e4d8 100644 --- a/modules.json +++ b/modules.json @@ -461,17 +461,17 @@ }, "tabix/bgzip": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "09d3c8c29b31a2dfd610305b10550f0e1dbcd4a9", "installed_by": ["modules"] }, "tabix/bgziptabix": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "09d3c8c29b31a2dfd610305b10550f0e1dbcd4a9", "installed_by": ["modules"] }, "tabix/tabix": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "09d3c8c29b31a2dfd610305b10550f0e1dbcd4a9", "installed_by": ["modules"] }, "tiddit/cov": { diff --git a/modules/local/mt_deletion_script.nf b/modules/local/mt_deletion_script.nf index f363b949..0da71506 100644 --- a/modules/local/mt_deletion_script.nf +++ b/modules/local/mt_deletion_script.nf @@ -2,10 +2,10 @@ process MT_DELETION { tag "$meta.id" label 'process_single' - conda "bioconda::samtools=1.17" + conda "bioconda::samtools=1.19.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : + 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" input: tuple val(meta), path(input), path(input_index) diff --git a/modules/nf-core/tabix/bgzip/environment.yml b/modules/nf-core/tabix/bgzip/environment.yml index 4fe40c56..361c078b 100644 --- a/modules/nf-core/tabix/bgzip/environment.yml +++ b/modules/nf-core/tabix/bgzip/environment.yml @@ -5,3 +5,4 @@ channels: - defaults dependencies: - bioconda::tabix=1.11 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/tabix/bgzip/main.nf b/modules/nf-core/tabix/bgzip/main.nf index 7772e9ad..3065dab0 100644 --- a/modules/nf-core/tabix/bgzip/main.nf +++ b/modules/nf-core/tabix/bgzip/main.nf @@ -4,8 +4,8 @@ process TABIX_BGZIP { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : - 'biocontainers/tabix:1.11--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/htslib:1.19.1--h81da01d_1' : + 'biocontainers/htslib:1.19.1--h81da01d_1' }" input: tuple val(meta), path(input) @@ -44,7 +44,8 @@ process TABIX_BGZIP { output = in_bgzip ? input.getBaseName() : "${prefix}.${input.getExtension()}.gz" """ - touch ${output} + echo "" | gzip > ${output} + touch ${output}.gzi cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/tabix/bgzip/tests/bgzip_compress.config b/modules/nf-core/tabix/bgzip/tests/bgzip_compress.config new file mode 100644 index 00000000..6b6ff55f --- /dev/null +++ b/modules/nf-core/tabix/bgzip/tests/bgzip_compress.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_BGZIP { + ext.args = ' -i' + } +} diff --git a/modules/nf-core/tabix/bgzip/tests/main.nf.test b/modules/nf-core/tabix/bgzip/tests/main.nf.test new file mode 100644 index 00000000..95fd4c50 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/tests/main.nf.test @@ -0,0 +1,111 @@ +nextflow_process { + + name "Test Process TABIX_BGZIP" + script "modules/nf-core/tabix/bgzip/main.nf" + process "TABIX_BGZIP" + + tag "modules" + tag "modules_nfcore" + tag "tabix" + tag "tabix/bgzip" + + test("sarscov2_vcf_bgzip_compress") { + when { + process { + """ + input[0] = [ + [ id:'bgzip_test' ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.output[0][1]).name + ).match("bgzip_test") + } + ) + } + } + + test("homo_genome_bedgz_compress") { + when { + process { + """ + input[0] = [ + [ id:'bedgz_test' ], + [ file(params.test_data['homo_sapiens']['genome']['genome_bed_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.output[0][1]).name + ).match("bedgz_test") + } + ) + } + } + + test("sarscov2_vcf_bgzip_compress_stub") { + options '-stub' + config "./bgzip_compress.config" + + when { + process { + """ + input[0] = [ + [ id:"test_stub" ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.output[0][1]).name + ).match("test_stub") + } + ) + } + } + + test("sarscov2_vcf_bgzip_compress_gzi") { + config "./bgzip_compress.config" + when { + process { + """ + input[0] = [ + [ id:"gzi_compress_test" ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.gzi[0][1]).name + ).match("gzi_compress_test") + } + ) + } + } +} diff --git a/modules/nf-core/tabix/bgzip/tests/main.nf.test.snap b/modules/nf-core/tabix/bgzip/tests/main.nf.test.snap new file mode 100644 index 00000000..53d59932 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/tests/main.nf.test.snap @@ -0,0 +1,186 @@ +{ + "gzi_compress_test": { + "content": [ + "gzi_compress_test.vcf.gz.gzi" + ], + "timestamp": "2024-02-19T14:52:29.328146" + }, + "homo_genome_bedgz_compress": { + "content": [ + { + "0": [ + [ + { + "id": "bedgz_test" + }, + "bedgz_test.bed:md5,87a15eb9c2ff20ccd5cd8735a28708f7" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ], + "gzi": [ + + ], + "output": [ + [ + { + "id": "bedgz_test" + }, + "bedgz_test.bed:md5,87a15eb9c2ff20ccd5cd8735a28708f7" + ] + ], + "versions": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ] + } + ], + "timestamp": "2024-02-19T14:52:12.422209" + }, + "test_stub": { + "content": [ + "test_stub.vcf.gz" + ], + "timestamp": "2024-02-19T14:52:20.811489" + }, + "sarscov2_vcf_bgzip_compress": { + "content": [ + { + "0": [ + [ + { + "id": "bgzip_test" + }, + "bgzip_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ], + "gzi": [ + + ], + "output": [ + [ + { + "id": "bgzip_test" + }, + "bgzip_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "versions": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ] + } + ], + "timestamp": "2024-02-19T14:52:03.706028" + }, + "sarscov2_vcf_bgzip_compress_gzi": { + "content": [ + { + "0": [ + [ + { + "id": "gzi_compress_test" + }, + "gzi_compress_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "1": [ + [ + { + "id": "gzi_compress_test" + }, + "gzi_compress_test.vcf.gz.gzi:md5,26fd00d4e26141cd11561f6e7d4a2ad0" + ] + ], + "2": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ], + "gzi": [ + [ + { + "id": "gzi_compress_test" + }, + "gzi_compress_test.vcf.gz.gzi:md5,26fd00d4e26141cd11561f6e7d4a2ad0" + ] + ], + "output": [ + [ + { + "id": "gzi_compress_test" + }, + "gzi_compress_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "versions": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ] + } + ], + "timestamp": "2024-02-19T14:52:29.271494" + }, + "bgzip_test": { + "content": [ + "bgzip_test.vcf.gz" + ], + "timestamp": "2024-02-19T14:52:03.768295" + }, + "bedgz_test": { + "content": [ + "bedgz_test.bed" + ], + "timestamp": "2024-02-19T14:52:12.453855" + }, + "sarscov2_vcf_bgzip_compress_stub": { + "content": [ + { + "0": [ + [ + { + "id": "test_stub" + }, + "test_stub.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + [ + { + "id": "test_stub" + }, + "test_stub.vcf.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ], + "gzi": [ + [ + { + "id": "test_stub" + }, + "test_stub.vcf.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "output": [ + [ + { + "id": "test_stub" + }, + "test_stub.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ] + } + ], + "timestamp": "2024-02-19T14:52:20.769619" + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/bgzip/tests/tags.yml b/modules/nf-core/tabix/bgzip/tests/tags.yml new file mode 100644 index 00000000..de0eec86 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/tests/tags.yml @@ -0,0 +1,2 @@ +tabix/bgzip: + - "modules/nf-core/tabix/bgzip/**" diff --git a/modules/nf-core/tabix/bgzip/tests/vcf_none.config b/modules/nf-core/tabix/bgzip/tests/vcf_none.config new file mode 100644 index 00000000..f3a3c467 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/tests/vcf_none.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_BGZIP { + ext.args = '' + } +} diff --git a/modules/nf-core/tabix/bgziptabix/environment.yml b/modules/nf-core/tabix/bgziptabix/environment.yml index 028461c9..c4235872 100644 --- a/modules/nf-core/tabix/bgziptabix/environment.yml +++ b/modules/nf-core/tabix/bgziptabix/environment.yml @@ -5,3 +5,4 @@ channels: - defaults dependencies: - bioconda::tabix=1.11 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/tabix/bgziptabix/main.nf b/modules/nf-core/tabix/bgziptabix/main.nf index f9482690..bcdcf2a6 100644 --- a/modules/nf-core/tabix/bgziptabix/main.nf +++ b/modules/nf-core/tabix/bgziptabix/main.nf @@ -4,8 +4,8 @@ process TABIX_BGZIPTABIX { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : - 'biocontainers/tabix:1.11--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/htslib:1.19.1--h81da01d_1' : + 'biocontainers/htslib:1.19.1--h81da01d_1' }" input: tuple val(meta), path(input) @@ -35,7 +35,7 @@ process TABIX_BGZIPTABIX { stub: def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${prefix}.${input.getExtension()}.gz + echo "" | gzip > ${prefix}.${input.getExtension()}.gz touch ${prefix}.${input.getExtension()}.gz.tbi touch ${prefix}.${input.getExtension()}.gz.csi diff --git a/modules/nf-core/tabix/bgziptabix/tests/main.nf.test b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test new file mode 100644 index 00000000..87ea2c84 --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test @@ -0,0 +1,94 @@ +nextflow_process { + + name "Test Process TABIX_BGZIPTABIX" + script "modules/nf-core/tabix/bgziptabix/main.nf" + process "TABIX_BGZIPTABIX" + + tag "modules" + tag "modules_nfcore" + tag "tabix" + tag "tabix/bgziptabix" + + test("sarscov2_bed_tbi") { + config "./tabix_tbi.config" + + when { + process { + """ + input[0] = [ + [ id:'tbi_test' ], + [ file(params.test_data['sarscov2']['genome']['test_bed'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.gz_tbi[0][1]).name + ).match("tbi_test") + } + ) + } + } + + test("sarscov2_bed_csi") { + config "./tabix_csi.config" + + when { + process { + """ + input[0] = [ + [ id:'csi_test' ], + [ file(params.test_data['sarscov2']['genome']['test_bed'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.gz_csi[0][1]).name + ).match("csi_test") + } + ) + } + + } + + test("sarscov2_bed_csi_stub") { + config "./tabix_csi.config" + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], + [ file(params.test_data['sarscov2']['genome']['test_bed'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.gz_csi[0][1]).name + ).match("csi_stub") + } + ) + } + + } + +} diff --git a/modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap new file mode 100644 index 00000000..fcecb2e4 --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap @@ -0,0 +1,143 @@ +{ + "sarscov2_bed_tbi": { + "content": [ + { + "0": [ + [ + { + "id": "tbi_test" + }, + "tbi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74", + "tbi_test.bed.gz.tbi:md5,24908545311cf2b7c803c41d716872c4" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,b4765e4d896ce4a4cdd6c896d12555fc" + ], + "gz_csi": [ + + ], + "gz_tbi": [ + [ + { + "id": "tbi_test" + }, + "tbi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74", + "tbi_test.bed.gz.tbi:md5,24908545311cf2b7c803c41d716872c4" + ] + ], + "versions": [ + "versions.yml:md5,b4765e4d896ce4a4cdd6c896d12555fc" + ] + } + ], + "timestamp": "2024-02-19T14:50:51.513838" + }, + "sarscov2_bed_csi": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "csi_test" + }, + "csi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74", + "csi_test.bed.gz.csi:md5,e06165ddd34640783728cb07f2558b43" + ] + ], + "2": [ + "versions.yml:md5,b4765e4d896ce4a4cdd6c896d12555fc" + ], + "gz_csi": [ + [ + { + "id": "csi_test" + }, + "csi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74", + "csi_test.bed.gz.csi:md5,e06165ddd34640783728cb07f2558b43" + ] + ], + "gz_tbi": [ + + ], + "versions": [ + "versions.yml:md5,b4765e4d896ce4a4cdd6c896d12555fc" + ] + } + ], + "timestamp": "2024-02-19T14:51:00.513777" + }, + "csi_test": { + "content": [ + "csi_test.bed.gz" + ], + "timestamp": "2024-02-19T14:51:00.548801" + }, + "csi_stub": { + "content": [ + "test.bed.gz" + ], + "timestamp": "2024-02-19T14:51:09.218454" + }, + "tbi_test": { + "content": [ + "tbi_test.bed.gz" + ], + "timestamp": "2024-02-19T14:50:51.579654" + }, + "sarscov2_bed_csi_stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940", + "test.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940", + "test.bed.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,b4765e4d896ce4a4cdd6c896d12555fc" + ], + "gz_csi": [ + [ + { + "id": "test" + }, + "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940", + "test.bed.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "gz_tbi": [ + [ + { + "id": "test" + }, + "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940", + "test.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,b4765e4d896ce4a4cdd6c896d12555fc" + ] + } + ], + "timestamp": "2024-02-19T14:51:09.164254" + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config b/modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config new file mode 100644 index 00000000..fb41a314 --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_BGZIPTABIX { + ext.args2 = '-p vcf --csi' + } +} diff --git a/modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config b/modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config new file mode 100644 index 00000000..c1915dc4 --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_BGZIPTABIX { + ext.args2 = '-p vcf' + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/bgziptabix/tests/tags.yml b/modules/nf-core/tabix/bgziptabix/tests/tags.yml new file mode 100644 index 00000000..5052b4d7 --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/tests/tags.yml @@ -0,0 +1,2 @@ +tabix/bgziptabix: + - "modules/nf-core/tabix/bgziptabix/**" diff --git a/modules/nf-core/tabix/tabix/environment.yml b/modules/nf-core/tabix/tabix/environment.yml index 7167fb87..76b45e16 100644 --- a/modules/nf-core/tabix/tabix/environment.yml +++ b/modules/nf-core/tabix/tabix/environment.yml @@ -5,3 +5,4 @@ channels: - defaults dependencies: - bioconda::tabix=1.11 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/tabix/tabix/main.nf b/modules/nf-core/tabix/tabix/main.nf index c304a8a3..f5e582d2 100644 --- a/modules/nf-core/tabix/tabix/main.nf +++ b/modules/nf-core/tabix/tabix/main.nf @@ -4,8 +4,8 @@ process TABIX_TABIX { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : - 'biocontainers/tabix:1.11--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/htslib:1.19.1--h81da01d_1' : + 'biocontainers/htslib:1.19.1--h81da01d_1' }" input: tuple val(meta), path(tab) @@ -32,7 +32,8 @@ process TABIX_TABIX { stub: def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${tab}.tbi + touch ${prefix}.tbi + touch ${prefix}.csi cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/tabix/tabix/tests/main.nf.test b/modules/nf-core/tabix/tabix/tests/main.nf.test new file mode 100644 index 00000000..3a150c70 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/main.nf.test @@ -0,0 +1,142 @@ +nextflow_process { + + name "Test Process TABIX_TABIX" + script "modules/nf-core/tabix/tabix/main.nf" + process "TABIX_TABIX" + + tag "modules" + tag "modules_nfcore" + tag "tabix" + tag "tabix/tabix" + + test("sarscov2_bedgz_tbi") { + config "./tabix_bed.config" + when { + process { + """ + input[0] = [ + [ id:'tbi_bed' ], + [ file(params.test_data['sarscov2']['genome']['test_bed_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.tbi[0][1]).name + ).match("tbi_bed") + } + ) + } + } + + test("sarscov2_gff_tbi") { + config "./tabix_gff.config" + when { + process { + """ + input[0] = [ + [ id:'tbi_gff' ], + [ file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.tbi[0][1]).name + ).match("tbi_gff") + } + ) + } + + } + + test("sarscov2_vcf_tbi") { + config "./tabix_vcf_tbi.config" + when { + process { + """ + input[0] = [ + [ id:'tbi_vcf' ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.tbi[0][1]).name + ).match("tbi_vcf") + } + ) + } + + } + + test("sarscov2_vcf_csi") { + config "./tabix_vcf_csi.config" + when { + process { + """ + input[0] = [ + [ id:'vcf_csi' ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.csi[0][1]).name + ).match("vcf_csi") + } + ) + } + + } + + test("sarscov2_vcf_csi_stub") { + config "./tabix_vcf_csi.config" + options "-stub" + when { + process { + """ + input[0] = [ + [ id:'vcf_csi_stub' ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.csi[0][1]).name + ).match("vcf_csi_stub") + } + ) + } + + } + +} diff --git a/modules/nf-core/tabix/tabix/tests/main.nf.test.snap b/modules/nf-core/tabix/tabix/tests/main.nf.test.snap new file mode 100644 index 00000000..484ebe29 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/main.nf.test.snap @@ -0,0 +1,217 @@ +{ + "vcf_csi_stub": { + "content": [ + "vcf_csi_stub.csi" + ], + "timestamp": "2024-02-19T14:54:03.35052" + }, + "tbi_gff": { + "content": [ + "genome.gff3.gz.tbi" + ], + "timestamp": "2024-02-19T14:53:37.420216" + }, + "sarscov2_gff_tbi": { + "content": [ + { + "0": [ + [ + { + "id": "tbi_gff" + }, + "genome.gff3.gz.tbi:md5,53fc683fd217aae47ef10d23c52a9178" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ], + "csi": [ + + ], + "tbi": [ + [ + { + "id": "tbi_gff" + }, + "genome.gff3.gz.tbi:md5,53fc683fd217aae47ef10d23c52a9178" + ] + ], + "versions": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ] + } + ], + "timestamp": "2024-02-19T14:53:37.388157" + }, + "sarscov2_bedgz_tbi": { + "content": [ + { + "0": [ + [ + { + "id": "tbi_bed" + }, + "test.bed.gz.tbi:md5,0f17d85e7f0a042b2aa367b70df224f8" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ], + "csi": [ + + ], + "tbi": [ + [ + { + "id": "tbi_bed" + }, + "test.bed.gz.tbi:md5,0f17d85e7f0a042b2aa367b70df224f8" + ] + ], + "versions": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ] + } + ], + "timestamp": "2024-02-19T14:53:28.879408" + }, + "tbi_vcf": { + "content": [ + "test.vcf.gz.tbi" + ], + "timestamp": "2024-02-19T14:53:46.402522" + }, + "vcf_csi": { + "content": [ + "test.vcf.gz.csi" + ], + "timestamp": "2024-02-19T14:53:54.921189" + }, + "sarscov2_vcf_tbi": { + "content": [ + { + "0": [ + [ + { + "id": "tbi_vcf" + }, + "test.vcf.gz.tbi:md5,897f3f378a811b90e6dee56ce08d2bcf" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ], + "csi": [ + + ], + "tbi": [ + [ + { + "id": "tbi_vcf" + }, + "test.vcf.gz.tbi:md5,897f3f378a811b90e6dee56ce08d2bcf" + ] + ], + "versions": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ] + } + ], + "timestamp": "2024-02-19T14:53:46.370358" + }, + "sarscov2_vcf_csi_stub": { + "content": [ + { + "0": [ + [ + { + "id": "vcf_csi_stub" + }, + "vcf_csi_stub.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "vcf_csi_stub" + }, + "vcf_csi_stub.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,3d45df6d80883bad358631069a2940fd" + ], + "csi": [ + [ + { + "id": "vcf_csi_stub" + }, + "vcf_csi_stub.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "tbi": [ + [ + { + "id": "vcf_csi_stub" + }, + "vcf_csi_stub.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,3d45df6d80883bad358631069a2940fd" + ] + } + ], + "timestamp": "2024-02-19T14:54:03.310028" + }, + "sarscov2_vcf_csi": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "vcf_csi" + }, + "test.vcf.gz.csi:md5,0731ad6f40104d2bbb1a2cc478ef8f03" + ] + ], + "2": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ], + "csi": [ + [ + { + "id": "vcf_csi" + }, + "test.vcf.gz.csi:md5,0731ad6f40104d2bbb1a2cc478ef8f03" + ] + ], + "tbi": [ + + ], + "versions": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ] + } + ], + "timestamp": "2024-02-19T14:53:54.886876" + }, + "tbi_bed": { + "content": [ + "test.bed.gz.tbi" + ], + "timestamp": "2024-02-19T14:53:28.947628" + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/tabix/tests/tabix_bed.config b/modules/nf-core/tabix/tabix/tests/tabix_bed.config new file mode 100644 index 00000000..7ff05905 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tabix_bed.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_TABIX { + ext.args = '-p bed' + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/tabix/tests/tabix_gff.config b/modules/nf-core/tabix/tabix/tests/tabix_gff.config new file mode 100644 index 00000000..20c0a1e3 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tabix_gff.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_TABIX { + ext.args = '-p gff' + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config b/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config new file mode 100644 index 00000000..eb4f2d7e --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_TABIX { + ext.args = '-p vcf --csi' + } +} diff --git a/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config b/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config new file mode 100644 index 00000000..2774c8a9 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_TABIX { + ext.args = '-p vcf' + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/tabix/tests/tags.yml b/modules/nf-core/tabix/tabix/tests/tags.yml new file mode 100644 index 00000000..6eda0653 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tags.yml @@ -0,0 +1,2 @@ +tabix/tabix: + - "modules/nf-core/tabix/tabix/**" diff --git a/subworkflows/local/annotate_mobile_elements.nf b/subworkflows/local/annotate_mobile_elements.nf index 53fecd0a..0d22cd80 100644 --- a/subworkflows/local/annotate_mobile_elements.nf +++ b/subworkflows/local/annotate_mobile_elements.nf @@ -6,7 +6,6 @@ include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_FILTER } from '../../modules/nf-core/bc include { ENSEMBLVEP_VEP as ENSEMBLVEP_ME } from '../../modules/nf-core/ensemblvep/vep/main' include { PICARD_SORTVCF } from '../../modules/nf-core/picard/sortvcf/main' include { SVDB_QUERY as SVDB_QUERY_DB } from '../../modules/nf-core/svdb/query/main' -include { TABIX_BGZIPTABIX as BGZIP_TABIX_ME } from '../../modules/nf-core/tabix/bgziptabix/main' include { ANNOTATE_CSQ_PLI as ANNOTATE_CSQ_PLI_ME } from '../../subworkflows/local/annotate_consequence_pli.nf' include { GENERATE_CLINICAL_SET as GENERATE_CLINICAL_SET_ME } from '../../subworkflows/local/generate_clinical_set.nf' diff --git a/subworkflows/local/annotate_mt_snvs.nf b/subworkflows/local/annotate_mt_snvs.nf index f28744f6..393ac317 100644 --- a/subworkflows/local/annotate_mt_snvs.nf +++ b/subworkflows/local/annotate_mt_snvs.nf @@ -102,6 +102,7 @@ workflow ANNOTATE_MT_SNVS { ch_versions = ch_versions.mix(VCFANNO_MT.out.versions) ch_versions = ch_versions.mix(HMTNOTE_ANNOTATE.out.versions) ch_versions = ch_versions.mix(HAPLOGREP2_CLASSIFY_MT.out.versions) + ch_versions = ch_versions.mix(ZIP_TABIX_HMTNOTE.out.versions) emit: haplog = HAPLOGREP2_CLASSIFY_MT.out.txt // channel: [ val(meta), path(txt) ] diff --git a/subworkflows/local/call_repeat_expansions.nf b/subworkflows/local/call_repeat_expansions.nf index d910cdac..65437480 100644 --- a/subworkflows/local/call_repeat_expansions.nf +++ b/subworkflows/local/call_repeat_expansions.nf @@ -11,7 +11,6 @@ include { STRANGER } from '../../modules/nf- include { SAMTOOLS_SORT } from '../../modules/nf-core/samtools/sort/main' include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' include { SVDB_MERGE as SVDB_MERGE_REPEATS } from '../../modules/nf-core/svdb/merge/main' -include { TABIX_BGZIPTABIX as BGZIPTABIX_EXP } from '../../modules/nf-core/tabix/bgziptabix/main' include { TABIX_TABIX as INDEX_STRANGER } from '../../modules/nf-core/tabix/tabix/main' include { TABIX_TABIX as TABIX_EXP_RENAME } from '../../modules/nf-core/tabix/tabix/main' diff --git a/subworkflows/local/call_structural_variants.nf b/subworkflows/local/call_structural_variants.nf index ad45dc29..f590bb60 100644 --- a/subworkflows/local/call_structural_variants.nf +++ b/subworkflows/local/call_structural_variants.nf @@ -4,7 +4,6 @@ include { CALL_SV_MANTA } from './variant_calling/call_sv_manta' include { CALL_SV_MT } from './variant_calling/call_sv_MT' -include { CALL_SV_MT as CALL_SV_MT_SHIFT } from './variant_calling/call_sv_MT' include { CALL_SV_TIDDIT } from './variant_calling/call_sv_tiddit' include { SVDB_MERGE } from '../../modules/nf-core/svdb/merge/main' include { CALL_SV_GERMLINECNVCALLER } from './variant_calling/call_sv_germlinecnvcaller' @@ -84,10 +83,12 @@ workflow CALL_STRUCTURAL_VARIANTS { TABIX_TABIX (SVDB_MERGE.out.vcf) + ch_versions = ch_versions.mix(CALL_SV_CNVNATOR.out.versions) ch_versions = ch_versions.mix(CALL_SV_MANTA.out.versions) ch_versions = ch_versions.mix(CALL_SV_MT.out.versions) ch_versions = ch_versions.mix(CALL_SV_TIDDIT.out.versions) ch_versions = ch_versions.mix(TABIX_TABIX.out.versions) + ch_versions = ch_versions.mix(SVDB_MERGE.out.versions) emit: vcf = SVDB_MERGE.out.vcf // channel: [ val(meta), path(vcf)] diff --git a/subworkflows/local/qc_bam.nf b/subworkflows/local/qc_bam.nf index 9bb5a2a4..b1ce5141 100644 --- a/subworkflows/local/qc_bam.nf +++ b/subworkflows/local/qc_bam.nf @@ -70,6 +70,7 @@ workflow QC_BAM { ch_cov = Channel.empty().mix(PICARD_COLLECTWGSMETRICS.out.metrics, SENTIEON_WGSMETRICS.out.wgs_metrics) ch_cov_y = Channel.empty().mix(PICARD_COLLECTWGSMETRICS_Y.out.metrics, SENTIEON_WGSMETRICS_Y.out.wgs_metrics) + ch_versions = ch_versions.mix(CHROMOGRAPH_COV.out.versions.first()) ch_versions = ch_versions.mix(PICARD_COLLECTMULTIPLEMETRICS.out.versions.first()) ch_versions = ch_versions.mix(PICARD_COLLECTHSMETRICS.out.versions.first()) ch_versions = ch_versions.mix(TIDDIT_COV.out.versions.first()) From 595de0e797301ee8fdb8a00485e68229672641f4 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 4 Mar 2024 13:03:53 +0100 Subject: [PATCH 362/387] update zenodo --- README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index 1cf7af5f..495ccea7 100644 --- a/README.md +++ b/README.md @@ -6,9 +6,9 @@ [![GitHub Actions CI Status](https://github.com/nf-core/raredisease/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/raredisease/actions/workflows/ci.yml) -[![GitHub Actions Linting Status](https://github.com/nf-core/raredisease/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/raredisease/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/raredisease/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![GitHub Actions Linting Status](https://github.com/nf-core/raredisease/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/raredisease/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/raredisease/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.7995798-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.7995798) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) -[![GitHub Actions Linting Status](https://github.com/nf-core/raredisease/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/raredisease/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/raredisease/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.7995798) +[![GitHub Actions Linting Status](https://github.com/nf-core/raredisease/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/raredisease/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/raredisease/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.7995798-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.7995798) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) From 9b7e402025c0f409cbe244a6aec9fdb0ba85f286 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 4 Mar 2024 13:28:18 +0100 Subject: [PATCH 363/387] fix lint --- .nf-core.yml | 1 + assets/nf-core-raredisease_logo_light.png | Bin 122212 -> 188284 bytes conf/modules/peddy_check.config | 26 ---------------------- conf/modules/raredisease.config | 14 ++++++++++++ modules/local/mt_deletion_script.nf | 1 - nextflow.config | 1 - subworkflows/local/peddy_check.nf | 22 ------------------ workflows/raredisease.nf | 10 ++++----- 8 files changed, 20 insertions(+), 55 deletions(-) delete mode 100644 conf/modules/peddy_check.config delete mode 100644 subworkflows/local/peddy_check.nf diff --git a/.nf-core.yml b/.nf-core.yml index 285d7803..0ceb0624 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -6,6 +6,7 @@ lint: - .github/PULL_REQUEST_TEMPLATE.md - docs/images/nf-core-raredisease_logo_dark.png - docs/images/nf-core-raredisease_logo_light.png + - assets/nf-core-raredisease_logo_light.png - assets/multiqc_config.yaml - .github/workflows/linting.yml - pyproject.toml diff --git a/assets/nf-core-raredisease_logo_light.png b/assets/nf-core-raredisease_logo_light.png index 163054c389f13eb2eac09d88c1aaaa5d99719db2..d6c4c283aa9557a1e8767df6f0e3e953e62f6048 100644 GIT binary patch literal 188284 zcmeFXcUP0y`#l^)MMuggDj;oCil_+EJB$SgRfvLg5GfKNlu$z*R8*RTNG~EyL_m5c 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zenr_LGtd-p=F!b}9dq|ay8@iirmU~Rgy1{TgxJ6mYDJ2TQTl&WbEktpaX;gREE*$K zpPudydHgjt;`JypZyXj_Z~1&|VdhRPx1aKT#AT}L*|Xs3XT{Dz#X5!(XZ6y{Up}Z0 zju~?sCWR~2UkLunKI^}B$#q+NcEWSb9y_Cp>NZAr filename.equals('versions.yml') ? null : filename } - ] - } -} diff --git a/conf/modules/raredisease.config b/conf/modules/raredisease.config index 2eae1c58..a16e6e0c 100644 --- a/conf/modules/raredisease.config +++ b/conf/modules/raredisease.config @@ -61,6 +61,20 @@ process { } } +// +// Peddy options +// + +process { + withName: '.*:PEDDY' { + publishDir = [ + path: { "${params.outdir}/peddy" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } +} + // // Multiqc options // diff --git a/modules/local/mt_deletion_script.nf b/modules/local/mt_deletion_script.nf index 0da71506..0702199a 100644 --- a/modules/local/mt_deletion_script.nf +++ b/modules/local/mt_deletion_script.nf @@ -2,7 +2,6 @@ process MT_DELETION { tag "$meta.id" label 'process_single' - conda "bioconda::samtools=1.19.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" diff --git a/nextflow.config b/nextflow.config index d8591f15..6982135b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -358,7 +358,6 @@ includeConfig 'conf/modules/call_structural_variants.config' includeConfig 'conf/modules/convert_mt_bam_to_fastq.config' includeConfig 'conf/modules/generate_cytosure_files.config' includeConfig 'conf/modules/gens.config' -includeConfig 'conf/modules/peddy_check.config' includeConfig 'conf/modules/prepare_references.config' includeConfig 'conf/modules/qc_bam.config' includeConfig 'conf/modules/rank_variants.config' diff --git a/subworkflows/local/peddy_check.nf b/subworkflows/local/peddy_check.nf deleted file mode 100644 index 101bc4a2..00000000 --- a/subworkflows/local/peddy_check.nf +++ /dev/null @@ -1,22 +0,0 @@ -// -// Peddy subworkflow to check sex and relatedness. -// - -include { PEDDY } from '../../modules/nf-core/peddy/main' - -workflow PEDDY_CHECK { - take: - ch_vcf // channel: [mandatory] [ val(meta), path(vcf), path(vcf_index) ] - ch_ped // channel: [mandatory] [ path(ped) ] - - main: - ch_versions = Channel.empty() - - PEDDY( ch_vcf, ch_ped ) - ch_versions = ch_versions.mix(PEDDY.out.versions.first()) - - emit: - ped = PEDDY.out.ped // channel: [ val(meta), path(ped) ] - csv = PEDDY.out.csv // channel: [ val(meta), path(csv) ] - versions = ch_versions // channel: [ versions.yml ] -} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 3375f536..32730897 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -103,6 +103,7 @@ if (missingParamsCount>0) { include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { PEDDY } from '../modules/nf-core/peddy/main' include { SMNCOPYNUMBERCALLER } from '../modules/nf-core/smncopynumbercaller/main' // @@ -133,7 +134,6 @@ include { GENERATE_CLINICAL_SET as GENERATE_CLINICAL_SET_SNV } from '../subworkf include { GENERATE_CLINICAL_SET as GENERATE_CLINICAL_SET_SV } from '../subworkflows/local/generate_clinical_set' include { GENERATE_CYTOSURE_FILES } from '../subworkflows/local/generate_cytosure_files' include { GENS } from '../subworkflows/local/gens' -include { PEDDY_CHECK } from '../subworkflows/local/peddy_check' include { PREPARE_REFERENCES } from '../subworkflows/local/prepare_references' include { QC_BAM } from '../subworkflows/local/qc_bam' include { RANK_VARIANTS as RANK_VARIANTS_MT } from '../subworkflows/local/rank_variants' @@ -611,11 +611,11 @@ workflow RAREDISEASE { // ped correspondence, sex check, ancestry check if (!params.skip_peddy) { - PEDDY_CHECK ( + PEDDY ( CALL_SNV.out.genome_vcf.join(CALL_SNV.out.genome_tabix, failOnMismatch:true, failOnDuplicate:true), ch_pedfile ) - ch_versions = ch_versions.mix(PEDDY_CHECK.out.versions) + ch_versions = ch_versions.mix(PEDDY.out.versions.first()) } // Generate CGH files from sequencing data, turned off by default @@ -703,8 +703,8 @@ workflow RAREDISEASE { ch_multiqc_files = ch_multiqc_files.mix(QC_BAM.out.cov.map{it[1]}.collect().ifEmpty([])) if (!params.skip_peddy) { - ch_multiqc_files = ch_multiqc_files.mix(PEDDY_CHECK.out.ped.map{it[1]}.collect().ifEmpty([])) - ch_multiqc_files = ch_multiqc_files.mix(PEDDY_CHECK.out.csv.map{it[1]}.collect().ifEmpty([])) + ch_multiqc_files = ch_multiqc_files.mix(PEDDY.out.ped.map{it[1]}.collect().ifEmpty([])) + ch_multiqc_files = ch_multiqc_files.mix(PEDDY.out.csv.map{it[1]}.collect().ifEmpty([])) } MULTIQC ( From fffc70284316279111e38531c662b4b2847a086e Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 4 Mar 2024 16:10:29 +0100 Subject: [PATCH 364/387] module update --- modules.json | 2 +- modules/nf-core/tabix/tabix/main.nf | 5 ++--- .../nf-core/tabix/tabix/tests/main.nf.test.snap | 14 +++++++------- 3 files changed, 10 insertions(+), 11 deletions(-) diff --git a/modules.json b/modules.json index b863e4d8..123924da 100644 --- a/modules.json +++ b/modules.json @@ -471,7 +471,7 @@ }, "tabix/tabix": { "branch": "master", - "git_sha": "09d3c8c29b31a2dfd610305b10550f0e1dbcd4a9", + "git_sha": "9502adb23c0b97ed8e616bbbdfa73b4585aec9a1", "installed_by": ["modules"] }, "tiddit/cov": { diff --git a/modules/nf-core/tabix/tabix/main.nf b/modules/nf-core/tabix/tabix/main.nf index f5e582d2..1737141d 100644 --- a/modules/nf-core/tabix/tabix/main.nf +++ b/modules/nf-core/tabix/tabix/main.nf @@ -30,10 +30,9 @@ process TABIX_TABIX { """ stub: - def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${prefix}.tbi - touch ${prefix}.csi + touch ${tab}.tbi + touch ${tab}.csi cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/tabix/tabix/tests/main.nf.test.snap b/modules/nf-core/tabix/tabix/tests/main.nf.test.snap index 484ebe29..034e38b6 100644 --- a/modules/nf-core/tabix/tabix/tests/main.nf.test.snap +++ b/modules/nf-core/tabix/tabix/tests/main.nf.test.snap @@ -1,9 +1,9 @@ { "vcf_csi_stub": { "content": [ - "vcf_csi_stub.csi" + "test.vcf.gz.csi" ], - "timestamp": "2024-02-19T14:54:03.35052" + "timestamp": "2024-03-04T14:51:59.788002" }, "tbi_gff": { "content": [ @@ -136,7 +136,7 @@ { "id": "vcf_csi_stub" }, - "vcf_csi_stub.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "1": [ @@ -144,7 +144,7 @@ { "id": "vcf_csi_stub" }, - "vcf_csi_stub.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + "test.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "2": [ @@ -155,7 +155,7 @@ { "id": "vcf_csi_stub" }, - "vcf_csi_stub.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + "test.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "tbi": [ @@ -163,7 +163,7 @@ { "id": "vcf_csi_stub" }, - "vcf_csi_stub.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "versions": [ @@ -171,7 +171,7 @@ ] } ], - "timestamp": "2024-02-19T14:54:03.310028" + "timestamp": "2024-03-04T14:51:59.766184" }, "sarscov2_vcf_csi": { "content": [ From 22ee3400c5e96f245592b38a485c9bd8f6f134b2 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 4 Mar 2024 17:45:35 +0100 Subject: [PATCH 365/387] bump version --- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index f22d4141..d6e1ef01 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -3,9 +3,9 @@ custom_logo_url: https://github.com/nf-core/raredisease/ custom_logo_title: "nf-core/raredisease" report_comment: > - This report has been generated by the nf-core/raredisease + This report has been generated by the nf-core/raredisease analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-raredisease-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index 6982135b..0ea42f23 100644 --- a/nextflow.config +++ b/nextflow.config @@ -340,7 +340,7 @@ manifest { description = """call and score variants from WGS/WES of rare disease patients""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.2.0dev' + version = '2.0.0' doi = '' } From 2c449af88e81979704c990cab1abcfcf6dbde0bc Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 5 Mar 2024 12:08:59 +0100 Subject: [PATCH 366/387] update changelog --- CHANGELOG.md | 10 +--------- 1 file changed, 1 insertion(+), 9 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 3d852b52..3335d663 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.2.0 - Genie [xxxx-xx-xx] +## v2.0.0 - Asterix [xxxx-xx-xx] ### `Added` @@ -70,14 +70,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Fixed wrong process names when outputting versions in `ALIGN_SENTIEON` and `CALL_SNV`. - Fixed gens subworkflow [#515](https://github.com/nf-core/raredisease/pull/515) -### `Updated` - -| Tool | Old version | New version | -| ------------ | ----------- | ----------- | -| `filter_vep` | 107 | 110 | -| `multiqc` | 1.14 | 1.15 | -| `untar` | (grep 3.4) | (grep 3.11) | - ## v1.1.1 - Abu (Patch) [2023-07-26] ### `Fixed` From 7ff514035c794f11a8942bf1bcb61bd66aa7f788 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 5 Mar 2024 12:30:59 +0100 Subject: [PATCH 367/387] Update CHANGELOG.md --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 3335d663..6863992b 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.0.0 - Asterix [xxxx-xx-xx] +## 2.0.0 - Asterix [xxxx-xx-xx] ### `Added` From e556cd76f3a410f557a80957503e2c7dca3454ea Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 5 Mar 2024 16:34:17 +0100 Subject: [PATCH 368/387] test --- .github/workflows/download_pipeline.yml | 5 +++++ 1 file changed, 5 insertions(+) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 08622fd5..303ba228 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -16,9 +16,11 @@ on: - opened branches: - master + - dev pull_request_target: branches: - master + - dev env: NXF_ANSI_LOG: false @@ -30,6 +32,9 @@ jobs: - name: Install Nextflow uses: nf-core/setup-nextflow@v1 + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" From d18a68a99041e5304ce56735974ee8ef16b43473 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 5 Mar 2024 16:45:26 +0100 Subject: [PATCH 369/387] change test branch --- .github/workflows/download_pipeline.yml | 4 +--- 1 file changed, 1 insertion(+), 3 deletions(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 303ba228..37073479 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -10,16 +10,14 @@ on: testbranch: description: "The specific branch you wish to utilize for the test execution of nf-core download." required: true - default: "dev" + default: "test-download" pull_request: types: - opened branches: - - master - dev pull_request_target: branches: - - master - dev env: From 5d794d738606de64afc91557ae8af26994f9ad21 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 6 Mar 2024 08:25:37 +0100 Subject: [PATCH 370/387] Update download_pipeline.yml --- .github/workflows/download_pipeline.yml | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 37073479..3c7ee491 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -10,15 +10,15 @@ on: testbranch: description: "The specific branch you wish to utilize for the test execution of nf-core download." required: true - default: "test-download" + default: "dev" pull_request: types: - opened branches: - - dev + - master pull_request_target: branches: - - dev + - master env: NXF_ANSI_LOG: false From 0aeb54681ee53129df9fb43cafcdbb41fb1859ca Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 7 Mar 2024 12:37:49 +0100 Subject: [PATCH 371/387] review suggestions --- .nf-core.yml | 3 - CHANGELOG.md | 2 +- README.md | 7 +- assets/mobile_element_references_schema.json | 2 +- assets/schema_input.json | 9 +- assets/vep_plugin_files_schema.json | 2 +- docs/images/raredisease_metromap_dark.pdf | Bin 38296 -> 198727 bytes docs/images/raredisease_metromap_dark.png | Bin 263779 -> 403312 bytes docs/images/raredisease_metromap_dark.svg | 2787 ++++++++--------- docs/images/raredisease_metromap_light.pdf | Bin 0 -> 129860 bytes docs/images/raredisease_metromap_light.png | Bin 322695 -> 395562 bytes docs/images/raredisease_metromap_light.svg | 1837 ++++++----- docs/output.md | 10 +- docs/usage.md | 14 +- lib/CustomFunctions.groovy | 85 + nextflow.config | 2 +- nextflow_schema.json | 10 +- .../call_sv_germlinecnvcaller.nf | 23 +- workflows/raredisease.nf | 69 +- 19 files changed, 2248 insertions(+), 2614 deletions(-) create mode 100644 docs/images/raredisease_metromap_light.pdf create mode 100644 lib/CustomFunctions.groovy diff --git a/.nf-core.yml b/.nf-core.yml index 0ceb0624..07152a97 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -7,7 +7,4 @@ lint: - docs/images/nf-core-raredisease_logo_dark.png - docs/images/nf-core-raredisease_logo_light.png - assets/nf-core-raredisease_logo_light.png - - assets/multiqc_config.yaml - - .github/workflows/linting.yml - - pyproject.toml repository_type: pipeline diff --git a/CHANGELOG.md b/CHANGELOG.md index 6863992b..3ffa9fa4 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,7 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Added` - Use `nf-validation` plugin for parameter and samplesheet validation [#386](https://github.com/nf-core/raredisease/pull/386) -- A new parameter to skip filtering based on vep results [#416](https://github.com/nf-core/raredisease/pull/416) +- A new parameter `skip_vep_filter` to skip filtering based on vep results [#416](https://github.com/nf-core/raredisease/pull/416) - A `metromap` representating the core parts of the pipeline [#428](https://github.com/nf-core/raredisease/pull/428) - Metromap and logos for light and dark theme [#432](https://github.com/nf-core/raredisease/pull/432) - New parameters to skip qualimap and eklipse (`--skip_qualimap` and `--skip_eklipse`) [#436](https://github.com/nf-core/raredisease/pull/436) diff --git a/README.md b/README.md index 495ccea7..7f87ce0f 100644 --- a/README.md +++ b/README.md @@ -38,9 +38,10 @@ On release, automated continuous integration tests run the pipeline on a full-si ## Pipeline summary -

    - -

    + + + nf-core/raredisease workflow + **1. Metrics:** diff --git a/assets/mobile_element_references_schema.json b/assets/mobile_element_references_schema.json index 40e79825..a128dd42 100644 --- a/assets/mobile_element_references_schema.json +++ b/assets/mobile_element_references_schema.json @@ -18,7 +18,7 @@ "format": "file-path", "exists": true, "pattern": "^\\S+\\.bed$", - "errorMessage": "Bed file, cannot contain spaces and must have extension '.bed'" + "errorMessage": "BED file name cannot contain spaces and must have extension '.bed'" } }, "required": ["type", "path"] diff --git a/assets/schema_input.json b/assets/schema_input.json index d7be05f3..c253ac64 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -26,16 +26,15 @@ "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" }, "fastq_2": { + "type": "string", "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", "anyOf": [ { - "type": "string", "pattern": "^\\S+\\.f(ast)?q\\.gz$", "format": "file-path", "exists": true }, { - "type": "string", "maxLength": 0 } ] @@ -55,31 +54,29 @@ "errorMessage": "Phenotype must be provided and cannot contain spaces" }, "paternal_id": { + "type": "string", "errorMessage": "Paternal ID cannot contain spaces", "meta": ["paternal"], "default": "0", "anyOf": [ { - "type": "string", "pattern": "^\\S+$" }, { - "type": "string", "maxLength": 0 } ] }, "maternal_id": { + "type": "string", "errorMessage": "Maternal ID cannot contain spaces", "meta": ["maternal"], "default": "0", "anyOf": [ { - "type": "string", "pattern": "^\\S+$" }, { - "type": "string", "maxLength": 0 } ] diff --git a/assets/vep_plugin_files_schema.json b/assets/vep_plugin_files_schema.json index 55695b6e..5dca6d16 100644 --- a/assets/vep_plugin_files_schema.json +++ b/assets/vep_plugin_files_schema.json @@ -18,7 +18,7 @@ } ], "exists": true, - "errorMessage": "Path to vep plugin files and their indices" + "description": "Path to vep plugin files and their indices" } }, "required": ["vep_files"] diff --git a/docs/images/raredisease_metromap_dark.pdf b/docs/images/raredisease_metromap_dark.pdf index 7dbd15eef380612d64c2315d1c8c056ec74f469f..2f603e0c3d873e1c880cf4e7fa32ff8eac484bea 100644 GIT binary patch literal 198727 zcmeFY1yh_s(>A)ey9RfHySuwP1b3I z(OUiqJxkv>*ExJNU6*sP!_GQtD@j^Qg`ScScKT7^#E(`3c9Jp!)^xH&aJlFb)mWm` z!CZb}E=H74{`Vf6x-^dw1^6=$M)nUrHNqBWKWVCpvX;bpHJN5IPIKd7vJ5!+a)lDr z9jj<2%6!oZ^n|D@SHXiu@a?KykoZCc;^jt6uR5+unW`%jk#E)&V`okKc=A6VE4rM< zxmmhG64qVJw*L$g4GzG!UQWu^T`Z;fD)YE=5Lqkp=2`n^Y0nBvQc+spXeaWu8if;D zmnC8=&^`;NWipEjBVwxMMyMIn_&!Qsj~wdVC1p91f7@B$wm!mbpV?HHvk%icU}5tD z3sb==gG-)6K1nFZPSi*VH!Kery+`-aRZr>rqk_1dxWw0btbv6OvrdzPoc~94#YJ9H zGL1hE@kDB21h#yh$TS3oMs&>`GvhlM$`0n`jy(U+yix&*el)(!*`Dwo|F5${c?t@w zQ5WA*jV=3y8E7Wj3{evjggr#6EQ}AICzrR99L!@C_LVqlIe zs$%=}-@J|Y+5YRDx9C<7Qh{{_f$kU2hsWEi^Vywo2EUiybhl=K*F}Yorm08x813DV 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rohstrangerupdvcfannocaddvepgenmodcaddvephmtnotegenmodmanta + + +tiddit + + +cnvnatorvepsvdb-querygermlinecnvcallergenmodexpansionhuntermultiqcpicardtools+mosdepthvcfannofastqfastqbamreferencesbam vcf vcf vcf vcfbameklipsedefault path traversed by the pipelineusers can configure the pipeline to traverse this path instead of the defaultusers have the option to not run these toolsbam vcf vcf vcfreferencesrarediseasenf- core/ + height="53.599998" + x="474.39999" + y="-1904.0004" + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-family:'Maven Pro';-inkscape-font-specification:'Maven Pro Bold';fill:#ffffff" + transform="scale(1,-1)" /> vcfeklipsedefault path alternative pathskippable pathsmanta diff --git a/docs/images/raredisease_metromap_light.pdf b/docs/images/raredisease_metromap_light.pdf new file mode 100644 index 0000000000000000000000000000000000000000..8a772fc4b88412e6b04bb8f5811b9ae277f696ce GIT binary patch literal 129860 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ztipC=p&=3!snf~2f9T!I1#kOE68`u)`?vopDJ1_N=pS4f9&8OR{fTQ29quNH5&(_4JW?A3@vBlzil3;uaEt18%4Pu^`? z151A%PsxYLlN*LhB*7#9`Hn!ZK@X>`wOzwiY|r}j;K3l1Q~jvB?DTj>fm!+^SGo=8V^qk2Xjxyrf1XjfquK-MI0ctNRg+ zZQEvCYiT11?DX#~TektzfZMUtd$g!a=gzAP4Gs0Yciq*%b{KWzH;$zujBvy z>VM@m|2~cX6%+mYEdFO=BA0)EkpKE$#nwNMTPSAb2CTz_ls+{L)@0yqLm9n I=Ns<-Kix0)RsaA1 diff --git a/docs/images/raredisease_metromap_light.svg b/docs/images/raredisease_metromap_light.svg index d76b822c..23c45049 100644 --- a/docs/images/raredisease_metromap_light.svg +++ b/docs/images/raredisease_metromap_light.svg @@ -2,15 +2,15 @@ sentieon-bwasentieon-dedupmarkduplicatesbwamem2alignment to mitochondriaalignment to mitochondria (bwamem2/bwamem2/ sentieon-bwa)sentieon/bwamarkduplicatesmarkduplicatesmutect2mutect2markduplicatesmutect2alignment to shifted mitochondria (bwamem2/ sentieon-bwa)alignment to shifted mitochondriasentieon-dnascopesentieon-dnascopesentieon-dnamodelapplysentieon-dnamodelapplydeepvariantdeepvariantglnexusglnexusbcftools - rohbcftools - rohstrangerstrangerupdupdvcfannovcfannocaddcaddvepvepgenmodgenmodcaddcaddvepvephmtnotehmtnotegenmodgenmodmanta + tiddit + cnvnatormanta + + +tiddit + + +cnvnatorvepvepsvdb-querysvdb-querygenmodgermlinecnvcallermanta + tiddit + cnvnator + germlinecnvcallergenmodexpansionhunterexpansionhuntermultiqcmultiqcpicardtools+mosdepthvcfannofastqfastqreferencesvcfannoeklipseeklipsedefault path traversed by the pipelineusers can configure the pipeline to traverse this path instead of the defaultdefault path alternative pathskippable pathssentieon-bwasentieon-dedupmarkduplicatesbwamem2bwausers have the option to not run these toolsfastqfastqreferences + style="fill:none;stroke:#24b064;stroke-width:4.36;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1" + d="M 183.85438,241.09504 H 104.07885" + id="path2500-6-0-8-3" />manta diff --git a/docs/output.md b/docs/output.md index 02258209..a0053784 100644 --- a/docs/output.md +++ b/docs/output.md @@ -89,7 +89,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d ##### Sentieon bwa mem -[Sentieon's bwa mem](https://support.sentieon.com/manual/DNAseq_usage/dnaseq/#map-reads-to-reference) is the software accelerated version of the bwa-mem algorithm. It is used to efficiently perform the alignment using BWA. Aligned reads are then coordinate sorted using Sentieon's [sort](https://support.sentieon.com/manual/usages/general/#util-syntax) utility. These files are treated as intermediates and are not placed in the output folder by default. It is not the default aligner, but it can be chosen by setting `--aligner` option to sentieon. +[Sentieon's bwa mem](https://support.sentieon.com/manual/DNAseq_usage/dnaseq/#map-reads-to-reference) is the software accelerated version of the bwa-mem algorithm. It is used to efficiently perform the alignment using BWA. Aligned reads are then coordinate sorted using Sentieon's [sort](https://support.sentieon.com/manual/usages/general/#util-syntax) utility. These files are treated as intermediates and are not placed in the output folder by default. It is not the default aligner, but it can be chosen by setting `--aligner` option to "sentieon". #### Duplicate marking @@ -304,7 +304,7 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support. #### SVDB merge -[SVDB merge](https://github.com/J35P312/SVDB#merge) is used to merge the variant calls from GATK's GermlineCNVCaller (only if skip_germlinecnvcaller is set to false), Manta, and TIDDIT. Output files are published in the output folder. +[SVDB merge](https://github.com/J35P312/SVDB#merge) is used to merge the variant calls from GATK's GermlineCNVCaller (only if `skip_germlinecnvcaller` is set to false), Manta, and TIDDIT. Output files are published in the output folder.

    Output files @@ -352,7 +352,7 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support. [bcftools roh](https://samtools.github.io/bcftools/bcftools.html#roh) is a program for detecting runs of homo/autozygosity.from only bi-allelic sites. The output files are not published in the output folder, and is passed to vcfanno for further annotation. :::note -In the case of running a quattro, i.e. two affected children and their parents, only one of the probands will be used for annotating regions of homozygosity. This is a know limitation that we are hoping to solve in a future release. +In the case of running a quattro, i.e. two affected children and their parents, only one of the probands will be used for annotating regions of homozygosity. This is a known limitation that we are hoping to solve in a future release. ::: #### vcfanno @@ -518,7 +518,7 @@ We recommend using vcfanno to annotate SNVs with precomputed CADD scores (files #### GENMOD -[GENMOD](https://github.com/Clinical-Genomics/genmod) is a simple to use command line tool for annotating and analyzing genomic variations in the VCF file format. GENMOD can annotate genetic patterns of inheritance in vcf:s with single or multiple families of arbitrary size. Each variant will be assigned a predicted pathogenicity score. The score will be given both as a raw score and a normalized score with values between 0 and 1. The tags in the INFO field are `RankScore` and `RankScoreNormalized`. The score can be configured to fit your annotations and preferences by modifying the score config file. +[GENMOD](https://github.com/Clinical-Genomics/genmod) is a simple to use command line tool for annotating and analyzing genomic variations in the VCF file format. GENMOD can annotate genetic patterns of inheritance in vcf files with single or multiple families of arbitrary size. Each variant will be assigned a predicted pathogenicity score. The score will be given both as a raw score and a normalized score with values between 0 and 1. The tags in the INFO field are `RankScore` and `RankScoreNormalized`. The score can be configured to fit your annotations and preferences by modifying the score config file.
    Output files @@ -556,7 +556,7 @@ Mobile elements are identified from the bam file using [RetroSeq](https://github #### Annotating mobile elements -The mobile elements are annotated with allele frequencies and allele counts using SVDB. These annotation files needed are preferably produced from a representative population. Further annoation is done using VEP and the resulting VCF is filtered using bcftools. The default filter is to only keep elements with `PASS` in the filter column but if no other post-processing is done we reccomend supplementing with an exclude expression based on population allele frequencies. The filtering key is dependent on the annotation files used but an example expression could look like this: `--exclude 'INFO/swegen_sva_FRQ > 0.1'`. If a list of HGNC id:s have been supplied with the option `--vep_filters`, variants matching those id:s will be presented in a seperate file using [filter_vep from VEP](https://www.ensembl.org/info/docs/tools/vep/script/vep_filter.html). This option can be disabled using the flag `--skip_vep_filter`. A VCF corresponding to the complete set of variants will also be produced. +The mobile elements are annotated with allele frequencies and allele counts using SVDB. These annotation files needed are preferably produced from a representative population. Further annoation is done using VEP and the resulting VCF is filtered using bcftools. The default filter is to only keep elements with `PASS` in the filter column but if no other post-processing is done we reccomend supplementing with an exclude expression based on population allele frequencies. The filtering key is dependent on the annotation files used but an example expression could look like this: `--exclude 'INFO/swegen_sva_FRQ > 0.1'`. If a list of HGNC id's have been supplied with the option `--vep_filters`, variants matching those id's will be presented in a seperate file using [filter_vep from VEP](https://www.ensembl.org/info/docs/tools/vep/script/vep_filter.html). This option can be disabled using the flag `--skip_vep_filter`. A VCF corresponding to the complete set of variants will also be produced.
    Output files diff --git a/docs/usage.md b/docs/usage.md index 878fe121..b419b28a 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -79,7 +79,9 @@ work # Directory containing the Nextflow working files Test profile runs the pipeline with a case containing three samples, but if you would like to test the pipeline with one sample, use `-profile test_one_sample,`. -> Note that the default cpu and memory configurations used in raredisease are written keeping the test profile (&dataset, which is tiny) in mind. You should override these values in configs to get it to work on larger datasets. Check the section `custom-configuration` below to know more about how to configure resources for your platform. +:::note +The default cpu and memory configurations used in raredisease are written keeping the test profile (&dataset, which is tiny) in mind. You should override these values in configs to get it to work on larger datasets. Check the section `custom-configuration` below to know more about how to configure resources for your platform. +::: ### Updating the pipeline @@ -146,7 +148,7 @@ nf-core/raredisease consists of several tools used for various purposes. For con 8. SV annotation & ranking (SVDB query, ensembl VEP, GENMOD) 9. Mitochondrial annotation -> We have only listed the groups that require at least one input from the user. For example, the pipeline also runs SMNCopyNumberCaller, but it does not require any input other than the bam files passed by the pipeline. Hence, it is not mentioned in the list above. To know more about the tools used in the pipeline check the [README](../README.md). +> We have only listed the groups that require at least one input from the user. For example, the pipeline also runs SMNCopyNumberCaller, but it does not require any input other than the bam files passed by the pipeline. Hence, it is not mentioned in the list above. To know more about the tools used in the pipeline check the [README](https://nf-co.re/raredisease). The mandatory and optional parameters for each category are tabulated below. @@ -165,7 +167,7 @@ The mandatory and optional parameters for each category are tabulated below. 1Default value is bwamem2. Other alternatives are bwa and sentieon (requires valid Sentieon license ).
    2Analysis set reference genome in fasta format, first 25 contigs need to be chromosome 1-22, X, Y and the mitochondria.
    -3f If mito_name is provided, mt_fasta can be generated by the pipeline.
    +3If mito_name is provided, mt_fasta can be generated by the pipeline.
    4fasta_fai, bwa and bwamem2, if not provided by the user, will be generated by the pipeline when necessary.
    5Used only by Sentieon.
    6Default value is 40. Used only by fastp.
    @@ -248,7 +250,9 @@ no header and the following columns: `CHROM POS REF_ALLELE ALT_ALLELE AF`. Sampl 10Path to a folder containing cadd annotations. Equivalent of the data/annotations/ folder described [here](https://github.com/kircherlab/CADD-scripts/#manual-installation), and it is used to calculate CADD scores for small indels.
    11A CSV file that describes the files used by VEP's named and custom plugins. Sample file [here](https://github.com/nf-core/test-datasets/blob/raredisease/reference/vep_files.csv).
    -> NB: We use CADD only to annotate small indels. To annotate SNVs with precomputed CADD scores, pass the file containing CADD scores as a resource to vcfanno instead. Files containing the precomputed CADD scores for SNVs can be downloaded from [here](https://cadd.gs.washington.edu/download) (description: "All possible SNVs of GRCh3<7/8>/hg3<7/8>") +:::note +We use CADD only to annotate small indels. To annotate SNVs with precomputed CADD scores, pass the file containing CADD scores as a resource to vcfanno instead. Files containing the precomputed CADD scores for SNVs can be downloaded from [here](https://cadd.gs.washington.edu/download) (download files listed under the description: "All possible SNVs of GRCh3<7/8>/hg3<7/8>") +::: ##### 8. SV annotation & Ranking @@ -408,8 +412,6 @@ Several generic profiles are bundled with the pipeline which instruct the pipeli We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. ::: -{%- if nf_core_configs %} - The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). {% else %} {% endif %} diff --git a/lib/CustomFunctions.groovy b/lib/CustomFunctions.groovy new file mode 100644 index 00000000..ddb27b30 --- /dev/null +++ b/lib/CustomFunctions.groovy @@ -0,0 +1,85 @@ +Class CustomFunctions { + + // Function to generate a pedigree file + def makePed(samples) { + + def case_name = samples[0].case_id + def outfile = file("${params.outdir}/pipeline_info/${case_name}" + '.ped') + outfile.text = ['#family_id', 'sample_id', 'father', 'mother', 'sex', 'phenotype'].join('\t') + def samples_list = [] + for(int i = 0; i meta} - ch_pedfile = ch_samples.toList().map { makePed(it) } + ch_pedfile = ch_samples.toList().map { CustomFunctions.makePed(it) } ch_case_info = ch_samples.toList().map { create_case_channel(it) } // Initialize file channels for PREPARE_REFERENCES subworkflow @@ -315,7 +315,7 @@ workflow RAREDISEASE { // Read and store hgnc ids in a channel ch_vep_filters_scout_fmt - .map { it -> parseHgncIds(it.text) } + .map { it -> CustomFunctions.parseHgncIds(it.text) } .mix (ch_vep_filters_std_fmt) .toList() .set {ch_hgnc_ids} @@ -719,71 +719,6 @@ workflow RAREDISEASE { versions = ch_versions // channel: [ path(versions.yml) ] } -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - FUNCTIONS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -def makePed(samples) { - - def case_name = samples[0].case_id - def outfile = file("${params.outdir}/pipeline_info/${case_name}" + '.ped') - outfile.text = ['#family_id', 'sample_id', 'father', 'mother', 'sex', 'phenotype'].join('\t') - def samples_list = [] - for(int i = 0; i Date: Thu, 7 Mar 2024 12:41:13 +0100 Subject: [PATCH 372/387] schema update --- assets/schema_input.json | 2 -- 1 file changed, 2 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index c253ac64..7bcd1812 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -42,14 +42,12 @@ "sex": { "type": "integer", "meta": ["sex"], - "pattern": "^\\S+$", "enum": [0, 1, 2], "errorMessage": "Sex must be provided and cannot contain spaces" }, "phenotype": { "type": "integer", "meta": ["phenotype"], - "pattern": "^\\S+$", "enum": [0, 1, 2], "errorMessage": "Phenotype must be provided and cannot contain spaces" }, From f06a9ac5d301a8f4972831901b90b948b0fb2981 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 7 Mar 2024 13:13:22 +0100 Subject: [PATCH 373/387] fix typo --- lib/CustomFunctions.groovy | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/lib/CustomFunctions.groovy b/lib/CustomFunctions.groovy index ddb27b30..a3a360a3 100644 --- a/lib/CustomFunctions.groovy +++ b/lib/CustomFunctions.groovy @@ -1,4 +1,4 @@ -Class CustomFunctions { +class CustomFunctions { // Function to generate a pedigree file def makePed(samples) { @@ -62,7 +62,7 @@ Class CustomFunctions { } // This function groups calls with same meta for postprocessing. - def reduce_input (List gcnvoutput) { + def reduce_input(List gcnvoutput) { def dictionary = [:] def reducedList = [] for (int i = 0; i Date: Thu, 7 Mar 2024 15:15:05 +0100 Subject: [PATCH 374/387] update customfunctions --- lib/CustomFunctions.groovy | 35 +++++-------------- .../call_sv_germlinecnvcaller.nf | 21 +++++++++++ workflows/raredisease.nf | 4 +-- 3 files changed, 31 insertions(+), 29 deletions(-) diff --git a/lib/CustomFunctions.groovy b/lib/CustomFunctions.groovy index a3a360a3..9ed0a9c0 100644 --- a/lib/CustomFunctions.groovy +++ b/lib/CustomFunctions.groovy @@ -1,14 +1,16 @@ +import nextflow.Nextflow + class CustomFunctions { // Function to generate a pedigree file - def makePed(samples) { + public static File makePed(samples, outdir) { def case_name = samples[0].case_id - def outfile = file("${params.outdir}/pipeline_info/${case_name}" + '.ped') + def outfile = new File(outdir +"/pipeline_info/${case_name}" + '.ped') outfile.text = ['#family_id', 'sample_id', 'father', 'mother', 'sex', 'phenotype'].join('\t') def samples_list = [] for(int i = 0; i meta} - ch_pedfile = ch_samples.toList().map { CustomFunctions.makePed(it) } - ch_case_info = ch_samples.toList().map { create_case_channel(it) } + ch_pedfile = ch_samples.toList().map { CustomFunctions.makePed(it, params.outdir) } + ch_case_info = ch_samples.toList().map { CustomFunctions.createCaseChannel(it) } // Initialize file channels for PREPARE_REFERENCES subworkflow ch_genome_fasta = Channel.fromPath(params.fasta).map { it -> [[id:it[0].simpleName], it] }.collect() From 51b4c52ac09540b62983ac261d148c85f0334dbe Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 7 Mar 2024 15:38:18 +0100 Subject: [PATCH 375/387] update changelog --- CHANGELOG.md | 49 +++++++++++++++++++++++++++++++++++++++++++++++++ 1 file changed, 49 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 3ffa9fa4..e61d9268 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -70,6 +70,55 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Fixed wrong process names when outputting versions in `ALIGN_SENTIEON` and `CALL_SNV`. - Fixed gens subworkflow [#515](https://github.com/nf-core/raredisease/pull/515) +### Parameters + +| Old parameter | New parameter | +| --------------------- | ------------------------------------- | +| | `--cnvnator_binsize` | +| | `--gens_pon_female` | +| | `--gens_pon_male` | +| | `--min_trimmed_length` | +| | `--mobile_element_references` | +| | `--mobile_element_svdb_annotations` | +| | `--mt_subsample_rd` | +| | `--mt_subsample_seed` | +| | `--ngsbits_samplegender_method` | +| | `--rtg_truthvcfs` | +| | `--run_rtgvcfeval` | +| | `--sample_id_map` | +| | `--score_config_mt` | +| | `--sdf` | +| `--pcr_amplification` | `--sentieon_dnascope_pcr_indel_model` | +| | `--skip_eklipse` | +| | `--skip_fastqc` | +| | `--skip_fastp` | +| | `--skip_gens` | +| | `--skip_germlinecnvcaller` | +| | `--skip_haplocheck` | +| | `--skip_me_annotation` | +| | `--skip_mt_annotation` | +| | `--skip_mt_subsample` | +| | `--skip_peddy` | +| | `--skip_qualimap` | +| | `--skip_vcf2cytosure` | +| | `--skip_vep_filter` | +| | `--svdb_query_bedpedbs` | +| | `--variant_consequences_snv` | +| | `--variant_consequences_sv` | +| | `--vcf2cytosure_blacklist` | +| | `--vep_plugin_files` | +| | `--vep_filters_scout_fmt` | +| `--gens_pon` | | +| `--gens_switch` | | +| `--skip_cnv_calling` | | +| `--skip_mt_analysis` | | + +:::note +Parameter has been updated if both old and new parameter information is present. +Parameter has been added if just the new parameter information is present. +Parameter has been removed if new parameter information isn't present. +::: + ## v1.1.1 - Abu (Patch) [2023-07-26] ### `Fixed` From 9f1d5bf5042aa2a4b1bfa703b1a178bd08c0f6dc Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 7 Mar 2024 15:52:01 +0100 Subject: [PATCH 376/387] update config --- conf/test.config | 2 +- conf/test_one_sample.config | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/test.config b/conf/test.config index fc849226..5301f96b 100644 --- a/conf/test.config +++ b/conf/test.config @@ -26,7 +26,7 @@ params { // analysis params skip_eklipse = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_fastqc = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI - skip_germlinecnvcaller = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_germlinecnvcaller = true skip_haplocheck = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_qualimap = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index 9ca23f70..404fe607 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -26,7 +26,7 @@ params { // analysis params skip_eklipse = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_fastqc = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI - skip_germlinecnvcaller = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI + skip_germlinecnvcaller = true skip_haplocheck = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_qualimap = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip tool on Github CI skip_mt_annotation = System.getenv("GITHUB_ACTIONS").equals(null) ? false : true // skip annotation on Github CI From 43d307644a241f4d1e042916d1ecdd9643aa5534 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 7 Mar 2024 16:27:48 +0100 Subject: [PATCH 377/387] fix errors --- nextflow_schema.json | 3 ++- workflows/raredisease.nf | 2 +- 2 files changed, 3 insertions(+), 2 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index b7b3f725..6e2fbc81 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -115,7 +115,8 @@ "description": "Name of iGenomes reference.", "fa_icon": "fas fa-align-center", "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details.", - "default": "GRCh38" + "default": "GRCh38", + "enum": ["GRCh37", "GRCh38"] }, "gens_gnomad_pos": { "type": "string", diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 001a660f..dbc1fcb6 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -160,7 +160,7 @@ workflow RAREDISEASE { ch_multiqc_files = Channel.empty() ch_samples = ch_samplesheet.map { meta, fastqs -> meta} - ch_pedfile = ch_samples.toList().map { CustomFunctions.makePed(it, params.outdir) } + ch_pedfile = ch_samples.toList().map { file(CustomFunctions.makePed(it, params.outdir)) } ch_case_info = ch_samples.toList().map { CustomFunctions.createCaseChannel(it) } // Initialize file channels for PREPARE_REFERENCES subworkflow From 48e8cf0424ec728f7df669c967dcd99570481b1a Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 7 Mar 2024 16:56:57 +0100 Subject: [PATCH 378/387] update maps --- docs/images/raredisease_metromap_dark.pdf | Bin 198727 -> 199627 bytes docs/images/raredisease_metromap_dark.png | Bin 403312 -> 429743 bytes docs/images/raredisease_metromap_dark.svg | 314 +++++++++++++-------- docs/images/raredisease_metromap_light.pdf | Bin 129860 -> 130756 bytes docs/images/raredisease_metromap_light.png | Bin 395562 -> 420122 bytes docs/images/raredisease_metromap_light.svg | 190 +++++++++---- 6 files changed, 338 insertions(+), 166 deletions(-) diff --git a/docs/images/raredisease_metromap_dark.pdf b/docs/images/raredisease_metromap_dark.pdf index 2f603e0c3d873e1c880cf4e7fa32ff8eac484bea..2a13f7e930aca33b4219195ef8ac189f13cdcca7 100644 GIT binary patch delta 13612 zcmV+{HPgz+kPOS043JQN97%HKJ%2?V*^;3;-jD0puE|VhI*hi>_RyvSCJ8h-lK|Ny zx;6Xn*FSfUM`jg3u;(U>+BB^A5b5FJ@pKOl4|_87-;JgJvC*4zHYFu{S|65Q7jw43 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ztMg2SV!;I|UpR8d2R-;KwJaML|LYG{o?2?WE>=DNZLXtc_PS@E)>o9pJM9sM)X3Z5 z+5b&Y2z0B3sp0M!l)aA5skKYjOge?OT1PSs-?&E)AG$5D4x8Ps8k%&uU7biN7WZSI zuaAsXXo;{RHYRjd-&Q)`KN(8V$mm$ztNwyQYYq+^oUv zQb4O?l1M^%Ba5(uJN0fFcdsoTTm6vp-6SL^6g}=amVap@>jIrTM7Ga(piS1>5C*T_ zGjuHQm`4Fp?6042FV4}ge{~1fU@4KH-MMq;upPI(M-6xhhFVn#KJ{ASkSldKh&T5X zMk8IJ7=42(R(EQ3JEN=j-h=bAsQhG%sLli)52dEN7ezxPBEi$3G^t}?IWZb={QvBk zmDB`fU>O6tkI)r#G6AsfJJD-tk!+{iE971UaO3wpCGj04gLbgzMn6^(1Im1O)zF2c9Ujc+Z}i z?%%+XzPntRAi;*Uv09T)rWDD73ww|VQ0$oZb1MiFLi&PRFffq`0pPI^7?*P?sCjea z1Y0}<16#SLiz9qM3adhxwoyr7NQ?%;Xdpl`#Ar$wO$nnZ0TKeFri8Em8IyPZxO2qs Qj1b5GPgg&ebxsLQ091W29smFU diff --git a/docs/images/raredisease_metromap_light.svg b/docs/images/raredisease_metromap_light.svg index 23c45049..da63879c 100644 --- a/docs/images/raredisease_metromap_light.svg +++ b/docs/images/raredisease_metromap_light.svg @@ -3,8 +3,8 @@ manta + id="tspan10">manta + + id="tspan11"> + tiddit + id="tspan12">tiddit + + id="tspan13"> + cnvnatorcnvnatorexpansionhuntermultiqcmultiqcpicardtools+mosdepthretroseq vcfdefault path default path alternative pathskippable pathsmantamanta + id="path10-04-6-7" />vepsvdb-query From 1353e069afa0d4608aea42379305a43c40554fe1 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 11 Mar 2024 09:41:08 +0100 Subject: [PATCH 379/387] fix? --- main.nf | 38 ++++++++++++++++++++++++++------------ nextflow.config | 47 ----------------------------------------------- 2 files changed, 26 insertions(+), 59 deletions(-) diff --git a/main.nf b/main.nf index fa0310ba..056ec813 100644 --- a/main.nf +++ b/main.nf @@ -11,18 +11,6 @@ nextflow.enable.dsl = 2 -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -include { RAREDISEASE } from './workflows/raredisease' -include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_raredisease_pipeline' -include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_raredisease_pipeline' - -include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_raredisease_pipeline' - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ GENOME PARAMETER VALUES @@ -53,11 +41,13 @@ params.ploidy_model = getGenomeAttribute('ploidy_model') params.reduced_penetrance = getGenomeAttribute('reduced_penetrance') params.readcount_intervals = getGenomeAttribute('readcount_intervals') params.rtg_truthvcfs = getGenomeAttribute('rtg_truthvcfs') +params.sample_id_map = getGenomeAttribute('sample_id_map') params.sequence_dictionary = getGenomeAttribute('sequence_dictionary') params.score_config_mt = getGenomeAttribute('score_config_mt') params.score_config_snv = getGenomeAttribute('score_config_snv') params.score_config_sv = getGenomeAttribute('score_config_sv') params.sdf = getGenomeAttribute('sdf') +params.svdb_query_bedpedbs = getGenomeAttribute('svdb_query_bedpedbs') params.svdb_query_dbs = getGenomeAttribute('svdb_query_dbs') params.target_bed = getGenomeAttribute('target_bed') params.variant_catalog = getGenomeAttribute('variant_catalog') @@ -71,6 +61,17 @@ params.vcfanno_toml = getGenomeAttribute('vcfanno_toml') params.vcfanno_lua = getGenomeAttribute('vcfanno_lua') params.vep_cache = getGenomeAttribute('vep_cache') params.vep_plugin_files = getGenomeAttribute('vep_plugin_files') + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { RAREDISEASE } from './workflows/raredisease' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_raredisease_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_raredisease_pipeline' + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ NAMED WORKFLOWS FOR PIPELINE @@ -147,3 +148,16 @@ workflow { THE END ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ + +// +// Get attribute from genome config file e.g. fasta +// + +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} diff --git a/nextflow.config b/nextflow.config index 1cfa2fad..b3f5e3e2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -48,53 +48,6 @@ params { // Bam_qc ngsbits_samplegender_method = 'xy' - // File params - fasta = null - fai = null - bwa = null - bwamem2 = null - call_interval = null - cadd_resources = null - gcnvcaller_model = null - gens_interval_list = null - gens_pon_female = null - gens_pon_male = null - gens_gnomad_pos = null - gnomad_af = null - gnomad_af_idx = null - intervals_wgs = null - intervals_y = null - known_dbsnp = null - known_dbsnp_tbi = null - mobile_element_references = null - mobile_element_svdb_annotations = null - ml_model = null - mt_fasta = null - ploidy_model = null - reduced_penetrance = null - readcount_intervals = null - rtg_truthvcfs = null - sample_id_map = null - sequence_dictionary = null - score_config_mt = null - score_config_snv = null - score_config_sv = null - sdf = null - svdb_query_bedpedbs = null - svdb_query_dbs = null - target_bed = null - variant_catalog = null - variant_consequences_snv = null - variant_consequences_sv = null - vep_filters = null - vep_filters_scout_fmt = null - vcf2cytosure_blacklist = null - vcfanno_resources = null - vcfanno_toml = null - vcfanno_lua = null - vep_cache = null - vep_plugin_files = null - // Alignment aligner = 'bwamem2' min_trimmed_length = 40 From c1d7666ebe9fd62ecacd59226ce70f1b52e6ffcc Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 11 Mar 2024 12:50:08 +0100 Subject: [PATCH 380/387] fix lint --- main.nf | 89 +++++++++++++++++++++++++++++---------------------------- 1 file changed, 45 insertions(+), 44 deletions(-) diff --git a/main.nf b/main.nf index 056ec813..aa92e744 100644 --- a/main.nf +++ b/main.nf @@ -17,50 +17,51 @@ nextflow.enable.dsl = 2 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -params.fasta = getGenomeAttribute('fasta') -params.fai = getGenomeAttribute('fai') -params.bwa = getGenomeAttribute('bwa') -params.bwamem2 = getGenomeAttribute('bwamem2') -params.call_interval = getGenomeAttribute('call_interval') -params.cadd_resources = getGenomeAttribute('cadd_resources') -params.gcnvcaller_model = getGenomeAttribute('gcnvcaller_model') -params.gens_interval_list = getGenomeAttribute('gens_interval_list') -params.gens_pon_female = getGenomeAttribute('gens_pon_female') -params.gens_pon_male = getGenomeAttribute('gens_pon_male') -params.gens_gnomad_pos = getGenomeAttribute('gens_gnomad_pos') -params.gnomad_af = getGenomeAttribute('gnomad_af') -params.gnomad_af_idx = getGenomeAttribute('gnomad_af_idx') -params.intervals_wgs = getGenomeAttribute('intervals_wgs') -params.intervals_y = getGenomeAttribute('intervals_y') -params.known_dbsnp = getGenomeAttribute('known_dbsnp') -params.known_dbsnp_tbi = getGenomeAttribute('known_dbsnp_tbi') -params.mobile_element_references = getGenomeAttribute('mobile_element_references') -params.ml_model = getGenomeAttribute('ml_model') -params.mt_fasta = getGenomeAttribute('mt_fasta') -params.ploidy_model = getGenomeAttribute('ploidy_model') -params.reduced_penetrance = getGenomeAttribute('reduced_penetrance') -params.readcount_intervals = getGenomeAttribute('readcount_intervals') -params.rtg_truthvcfs = getGenomeAttribute('rtg_truthvcfs') -params.sample_id_map = getGenomeAttribute('sample_id_map') -params.sequence_dictionary = getGenomeAttribute('sequence_dictionary') -params.score_config_mt = getGenomeAttribute('score_config_mt') -params.score_config_snv = getGenomeAttribute('score_config_snv') -params.score_config_sv = getGenomeAttribute('score_config_sv') -params.sdf = getGenomeAttribute('sdf') -params.svdb_query_bedpedbs = getGenomeAttribute('svdb_query_bedpedbs') -params.svdb_query_dbs = getGenomeAttribute('svdb_query_dbs') -params.target_bed = getGenomeAttribute('target_bed') -params.variant_catalog = getGenomeAttribute('variant_catalog') -params.variant_consequences_snv = getGenomeAttribute('variant_consequences_snv') -params.variant_consequences_sv = getGenomeAttribute('variant_consequences_sv') -params.vep_filters = getGenomeAttribute('vep_filters') -params.vep_filters_scout_fmt = getGenomeAttribute('vep_filters_scout_fmt') -params.vcf2cytosure_blacklist = getGenomeAttribute('vcf2cytosure_blacklist') -params.vcfanno_resources = getGenomeAttribute('vcfanno_resources') -params.vcfanno_toml = getGenomeAttribute('vcfanno_toml') -params.vcfanno_lua = getGenomeAttribute('vcfanno_lua') -params.vep_cache = getGenomeAttribute('vep_cache') -params.vep_plugin_files = getGenomeAttribute('vep_plugin_files') +params.fasta = getGenomeAttribute('fasta') +params.fai = getGenomeAttribute('fai') +params.bwa = getGenomeAttribute('bwa') +params.bwamem2 = getGenomeAttribute('bwamem2') +params.call_interval = getGenomeAttribute('call_interval') +params.cadd_resources = getGenomeAttribute('cadd_resources') +params.gcnvcaller_model = getGenomeAttribute('gcnvcaller_model') +params.gens_interval_list = getGenomeAttribute('gens_interval_list') +params.gens_pon_female = getGenomeAttribute('gens_pon_female') +params.gens_pon_male = getGenomeAttribute('gens_pon_male') +params.gens_gnomad_pos = getGenomeAttribute('gens_gnomad_pos') +params.gnomad_af = getGenomeAttribute('gnomad_af') +params.gnomad_af_idx = getGenomeAttribute('gnomad_af_idx') +params.intervals_wgs = getGenomeAttribute('intervals_wgs') +params.intervals_y = getGenomeAttribute('intervals_y') +params.known_dbsnp = getGenomeAttribute('known_dbsnp') +params.known_dbsnp_tbi = getGenomeAttribute('known_dbsnp_tbi') +params.mobile_element_references = getGenomeAttribute('mobile_element_references') +params.mobile_element_svdb_annotations = getGenomeAttribute('mobile_element_svdb_annotations') +params.ml_model = getGenomeAttribute('ml_model') +params.mt_fasta = getGenomeAttribute('mt_fasta') +params.ploidy_model = getGenomeAttribute('ploidy_model') +params.reduced_penetrance = getGenomeAttribute('reduced_penetrance') +params.readcount_intervals = getGenomeAttribute('readcount_intervals') +params.rtg_truthvcfs = getGenomeAttribute('rtg_truthvcfs') +params.sample_id_map = getGenomeAttribute('sample_id_map') +params.sequence_dictionary = getGenomeAttribute('sequence_dictionary') +params.score_config_mt = getGenomeAttribute('score_config_mt') +params.score_config_snv = getGenomeAttribute('score_config_snv') +params.score_config_sv = getGenomeAttribute('score_config_sv') +params.sdf = getGenomeAttribute('sdf') +params.svdb_query_bedpedbs = getGenomeAttribute('svdb_query_bedpedbs') +params.svdb_query_dbs = getGenomeAttribute('svdb_query_dbs') +params.target_bed = getGenomeAttribute('target_bed') +params.variant_catalog = getGenomeAttribute('variant_catalog') +params.variant_consequences_snv = getGenomeAttribute('variant_consequences_snv') +params.variant_consequences_sv = getGenomeAttribute('variant_consequences_sv') +params.vep_filters = getGenomeAttribute('vep_filters') +params.vep_filters_scout_fmt = getGenomeAttribute('vep_filters_scout_fmt') +params.vcf2cytosure_blacklist = getGenomeAttribute('vcf2cytosure_blacklist') +params.vcfanno_resources = getGenomeAttribute('vcfanno_resources') +params.vcfanno_toml = getGenomeAttribute('vcfanno_toml') +params.vcfanno_lua = getGenomeAttribute('vcfanno_lua') +params.vep_cache = getGenomeAttribute('vep_cache') +params.vep_plugin_files = getGenomeAttribute('vep_plugin_files') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From dc7610fda6419a9d77ca75c580d8f6147a677816 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 12 Mar 2024 08:54:43 +0100 Subject: [PATCH 381/387] fix reduce_input --- subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf b/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf index 50e07b88..a0636268 100644 --- a/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf +++ b/subworkflows/local/variant_calling/call_sv_germlinecnvcaller.nf @@ -41,7 +41,7 @@ workflow CALL_SV_GERMLINECNVCALLER { GATK4_GERMLINECNVCALLER ( ch_gcnvc_in ) GATK4_GERMLINECNVCALLER.out.calls.toList() - .flatMap {CustomFunctions.reduce_input(it)} + .flatMap {reduce_input(it)} .buffer (size: 2) .combine(ch_gcnvcaller_model.collect{it[1]}.toList()) .join(GATK4_DETERMINEGERMLINECONTIGPLOIDY.out.calls) From 7b40c581b76c36e2907d3dd044b2fb951e156960 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 12 Mar 2024 08:56:57 +0100 Subject: [PATCH 382/387] remove TODO --- subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf | 3 --- 1 file changed, 3 deletions(-) diff --git a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf index 5a39c012..c70f1783 100644 --- a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf @@ -226,7 +226,6 @@ def genomeExistsError() { // Generate methods description for MultiQC // def toolCitationText() { - // TODO nf-core: Optionally add in-text citation tools to this list. // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", // Uncomment function in methodsDescriptionText to render in MultiQC report def citation_text = [ @@ -240,7 +239,6 @@ def toolCitationText() { } def toolBibliographyText() { - // TODO nf-core: Optionally add bibliographic entries to this list. // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "

  • Author (2023) Pub name, Journal, DOI
    • " : "", // Uncomment function in methodsDescriptionText to render in MultiQC report def reference_text = [ @@ -265,7 +263,6 @@ def methodsDescriptionText(mqc_methods_yaml) { meta["tool_citations"] = "" meta["tool_bibliography"] = "" - // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") // meta["tool_bibliography"] = toolBibliographyText() From 4f198f2eefe2f3a860c4dc293f8e3ace6e15b885 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 13 Mar 2024 16:16:44 +0100 Subject: [PATCH 383/387] citations --- assets/multiqc_config.yml | 1 + .../utils_nfcore_raredisease_pipeline/main.nf | 238 ++++++++++++++++-- workflows/raredisease.nf | 2 +- 3 files changed, 216 insertions(+), 25 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index d6e1ef01..174bb344 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -21,6 +21,7 @@ run_modules: - qualimap - picard - mosdepth + - custom_content module_order: - fastqc: diff --git a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf index c70f1783..e2d24ec0 100644 --- a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf @@ -226,27 +226,220 @@ def genomeExistsError() { // Generate methods description for MultiQC // def toolCitationText() { - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text + + def align_text = [] + def variant_call_text = [] + def repeat_call_text = [] + def snv_annotation_text = [] + def sv_annotation_text = [] + def mt_annotation_text = [] + def qc_bam_text = [] + def me_calls_text = [] + def me_annotation_text = [] + def preprocessing_text = [] + def other_citation_text = [] + + align_text = [ + params.aligner.equals("bwa") ? "BWA (Li, 2013)," :"", + params.aligner.equals("bwamem2") ? "BWA-MEM2 (Vasimuddin et al., 2019)," : "", + params.aligner.equals("sentieon") ? "Sentieon DNASeq (Kendig et al., 2019)," : "", + params.aligner.equals("sentieon") ? "Sentieon Tools (Freed et al., 2017)," : "" + ] + variant_call_text = [ + params.variant_caller.equals("deepvariant") ? "DeepVariant (Poplin et al., 2018)," : "", + params.variant_caller.equals("sentieon") ? "Sentieon DNAscope (Freed et al., 2022)," : "", + params.skip_haplocheck ? "" : "Haplocheck (Weissensteiner et al., 2021),", + "CNVnator (Abyzov et al., 2011),", + "TIDDIT (Eisfeldt et al., 2017),", + "Manta (Chen et al., 2016),", + "GLnexus (Yun et al., 2021),", + params.skip_eklipse ? "" : "eKLIPse (Goudenge et al., 2019),", + ] + repeat_call_text = [ + "ExpansionHunter (Dolzhenko et al., 2019),", + "stranger (Nilsson & Magnusson, 2021)," + ] + if (!params.skip_snv_annotation) { + snv_annotation_text = [ + "CADD (Rentzsch et al., 2019, 2021),", + "Vcfanno (Pedersen et al., 2016),", + "VEP (McLaren et al., 2016),", + "Genmod (Magnusson et al., 2018),", + ] + } + if (!params.skip_sv_annotation) { + sv_annotation_text = [ + "SVDB (Eisfeldt et al., 2017),", + "VEP (McLaren et al., 2016),", + "Genmod (Magnusson et al., 2018),", + ] + } + if (!params.skip_mt_annotation) { + mt_annotation_text = [ + "CADD (Rentzsch et al., 2019, 2021),", + "VEP (McLaren et al., 2016),", + "Vcfanno (Pedersen et al., 2016),", + "Hmtnote (Preste et al., 2019),", + "HaploGrep2 (Weissensteiner et al., 2016),", + "Genmod (Magnusson et al., 2018),", + ] + } + if (!params.skip_me_annotation) { + me_annotation_text = [ + "VEP (McLaren et al., 2016),", + "SVDB (Eisfeldt et al., 2017),", + ] + } + qc_bam_text = [ + "Picard (Broad Institute, 2023)", + "Qualimap (Okonechnikov et al., 2016),", + "TIDDIT (Eisfeldt et al., 2017),", + "UCSC Bigwig and Bigbed (Kent et al., 2010),", + "Mosdepth (Pedersen & Quinlan, 2018),", + ] + me_calls_text = [ + "SVDB (Eisfeldt et al., 2017),", + "RetroSeq (Keane et al., 2013),", + ] + preprocessing_text = [ + params.skip_fastqc ? "" : "FastQC (Andrews 2010),", + params.skip_fastp ? "" : "Fastp (Chen, 2023),", + ] + other_citation_text = [ + "BCFtools (Danecek et al., 2021),", + "GATK (McKenna et al., 2010),", + "MultiQC (Ewels et al. 2016),", + params.skip_peddy ? "" : "Peddy (Pedersen & Quinlan, 2017),", + params.run_rtgvcfeval ? "RTG Tools (Cleary et al., 2015)," : "", + "SAMtools (Li et al., 2009),", + "SMNCopyNumberCaller (Chen et al., 2020),", + "Tabix (Li, 2011)", + "." + ] + + def concat_text = align_text + + variant_call_text + + repeat_call_text + + snv_annotation_text + + sv_annotation_text + + mt_annotation_text + + qc_bam_text + + me_calls_text + + me_annotation_text + + preprocessing_text + + other_citation_text + + def citation_text = [ "Tools used in the workflow included:" ] + concat_text.unique(false) { a, b -> a <=> b } - "" + return citation_text.join(' ').trim() } def toolBibliographyText() { - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text + + def align_text = [] + def variant_call_text = [] + def repeat_call_text = [] + def snv_annotation_text = [] + def sv_annotation_text = [] + def mt_annotation_text = [] + def qc_bam_text = [] + def me_calls_text = [] + def me_annotation_text = [] + def preprocessing_text = [] + def other_citation_text = [] + + align_text = [ + params.aligner.equals("bwa") ? "
  • Li, H. (2013). Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM (arXiv:1303.3997). arXiv. http://arxiv.org/abs/1303.3997
    • " :"", + params.aligner.equals("bwamem2") ? "
  • Vasimuddin, Md., Misra, S., Li, H., & Aluru, S. (2019). Efficient Architecture-Aware Acceleration of BWA-MEM for Multicore Systems. 2019 IEEE International Parallel and Distributed Processing Symposium (IPDPS), 314–324. https://doi.org/10.1109/IPDPS.2019.00041
    • " : "", + params.aligner.equals("sentieon") ? "
  • Kendig, K. I., Baheti, S., Bockol, M. A., Drucker, T. M., Hart, S. N., Heldenbrand, J. R., Hernaez, M., Hudson, M. E., Kalmbach, M. T., Klee, E. W., Mattson, N. R., Ross, C. A., Taschuk, M., Wieben, E. D., Wiepert, M., Wildman, D. E., & Mainzer, L. S. (2019). Sentieon DNASeq Variant Calling Workflow Demonstrates Strong Computational Performance and Accuracy. Frontiers in Genetics, 10, 736. https://doi.org/10.3389/fgene.2019.00736
    • " : "", + params.aligner.equals("sentieon") ? "
  • Freed, D., Aldana, R., Weber, J. A., & Edwards, J. S. (2017). The Sentieon Genomics Tools—A fast and accurate solution to variant calling from next-generation sequence data (p. 115717). bioRxiv. https://doi.org/10.1101/115717
    • " : "" + ] + variant_call_text = [ + params.variant_caller.equals("deepvariant") ? "
  • Poplin, R., Chang, P.-C., Alexander, D., Schwartz, S., Colthurst, T., Ku, A., Newburger, D., Dijamco, J., Nguyen, N., Afshar, P. T., Gross, S. S., Dorfman, L., McLean, C. Y., & DePristo, M. A. (2018). A universal SNP and small-indel variant caller using deep neural networks. Nature Biotechnology, 36(10), 983–987. https://doi.org/10.1038/nbt.4235
    • " : "", + params.variant_caller.equals("sentieon") ? "
  • Freed, D., Pan, R., Chen, H., Li, Z., Hu, J., & Aldana, R. (2022). DNAscope: High accuracy small variant calling using machine learning [Preprint]. Bioinformatics. https://doi.org/10.1101/2022.05.20.492556
    • " : "", + params.skip_haplocheck ? "" : "
  • Weissensteiner, H., Forer, L., Fendt, L., Kheirkhah, A., Salas, A., Kronenberg, F., & Schoenherr, S. (2021). Contamination detection in sequencing studies using the mitochondrial phylogeny. Genome Research, 31(2), 309–316. https://doi.org/10.1101/gr.256545.119
    • ", + "
  • Abyzov, A., Urban, A. E., Snyder, M., & Gerstein, M. (2011). CNVnator: An approach to discover, genotype, and characterize typical and atypical CNVs from family and population genome sequencing. Genome Research, 21(6), 974–984. https://doi.org/10.1101/gr.114876.110
    • ", + "
  • Eisfeldt, J., Vezzi, F., Olason, P., Nilsson, D., & Lindstrand, A. (2017). TIDDIT, an efficient and comprehensive structural variant caller for massive parallel sequencing data. F1000Research, 6, 664. https://doi.org/10.12688/f1000research.11168.2
    • ", + "
  • Chen, X., Schulz-Trieglaff, O., Shaw, R., Barnes, B., Schlesinger, F., Källberg, M., Cox, A. J., Kruglyak, S., & Saunders, C. T. (2016). Manta: Rapid detection of structural variants and indels for germline and cancer sequencing applications. Bioinformatics, 32(8), 1220–1222. https://doi.org/10.1093/bioinformatics/btv710
    • ", + "
  • Yun, T., Li, H., Chang, P.-C., Lin, M. F., Carroll, A., & McLean, C. Y. (2021). Accurate, scalable cohort variant calls using DeepVariant and GLnexus. Bioinformatics, 36(24), 5582–5589. https://doi.org/10.1093/bioinformatics/btaa1081
    • ", + params.skip_eklipse ? "" : "
  • Goudenège, D., Bris, C., Hoffmann, V., Desquiret-Dumas, V., Jardel, C., Rucheton, B., Bannwarth, S., Paquis-Flucklinger, V., Lebre, A. S., Colin, E., Amati-Bonneau, P., Bonneau, D., Reynier, P., Lenaers, G., & Procaccio, V. (2019). eKLIPse: A sensitive tool for the detection and quantification of mitochondrial DNA deletions from next-generation sequencing data. Genetics in Medicine, 21(6), 1407–1416. https://doi.org/10.1038/s41436-018-0350-8
    • ", + ] + repeat_call_text = [ + "
  • Dolzhenko, E., Deshpande, V., Schlesinger, F., Krusche, P., Petrovski, R., Chen, S., Emig-Agius, D., Gross, A., Narzisi, G., Bowman, B., Scheffler, K., van Vugt, J. J. F. A., French, C., Sanchis-Juan, A., Ibáñez, K., Tucci, A., Lajoie, B. R., Veldink, J. H., Raymond, F. L., … Eberle, M. A. (2019). ExpansionHunter: A sequence-graph-based tool to analyze variation in short tandem repeat regions. Bioinformatics, 35(22), 4754–4756. https://doi.org/10.1093/bioinformatics/btz431
    • ", + "
  • Nilsson, D., & Magnusson, M. (2021). Moonso/stranger v0.7.1 (v0.7.1) [Computer software]. Zenodo. https://doi.org/10.5281/ZENODO.4548873
    • " + ] + if (!params.skip_snv_annotation) { + snv_annotation_text = [ + "
  • Rentzsch, P., Schubach, M., Shendure, J., & Kircher, M. (2021). CADD-Splice—Improving genome-wide variant effect prediction using deep learning-derived splice scores. Genome Medicine, 13(1), 31. https://doi.org/10.1186/s13073-021-00835-9
    • ", + "
  • Rentzsch, P., Witten, D., Cooper, G. M., Shendure, J., & Kircher, M. (2019). CADD: Predicting the deleteriousness of variants throughout the human genome. Nucleic Acids Research, 47(D1), D886–D894. https://doi.org/10.1093/nar/gky1016
    • ", + "
  • Pedersen, B. S., Layer, R. M., & Quinlan, A. R. (2016). Vcfanno: Fast, flexible annotation of genetic variants. Genome Biology, 17(1), 118. https://doi.org/10.1186/s13059-016-0973-5
    • ", + "
  • McLaren, W., Gil, L., Hunt, S. E., Riat, H. S., Ritchie, G. R. S., Thormann, A., Flicek, P., & Cunningham, F. (2016). The Ensembl Variant Effect Predictor. Genome Biology, 17(1), 122. https://doi.org/10.1186/s13059-016-0974-4
    • ", + "
  • Magnusson, M., Hughes, T., Glabilloy, & Bitdeli Chef. (2018). genmod: Version 3.7.3 (3.7.3) [Computer software]. Zenodo. https://doi.org/10.5281/ZENODO.3841142
    • ", + ] + } + if (!params.skip_sv_annotation) { + sv_annotation_text = [ + "
  • Eisfeldt, J., Vezzi, F., Olason, P., Nilsson, D., & Lindstrand, A. (2017). TIDDIT, an efficient and comprehensive structural variant caller for massive parallel sequencing data. F1000Research, 6, 664. https://doi.org/10.12688/f1000research.11168.2
    • ", + "
  • McLaren, W., Gil, L., Hunt, S. E., Riat, H. S., Ritchie, G. R. S., Thormann, A., Flicek, P., & Cunningham, F. (2016). The Ensembl Variant Effect Predictor. Genome Biology, 17(1), 122. https://doi.org/10.1186/s13059-016-0974-4
    • ", + "
  • Magnusson, M., Hughes, T., Glabilloy, & Bitdeli Chef. (2018). genmod: Version 3.7.3 (3.7.3) [Computer software]. Zenodo. https://doi.org/10.5281/ZENODO.3841142
    • ", + ] + } + if (!params.skip_mt_annotation) { + mt_annotation_text = [ + "
  • Rentzsch, P., Schubach, M., Shendure, J., & Kircher, M. (2021). CADD-Splice—Improving genome-wide variant effect prediction using deep learning-derived splice scores. Genome Medicine, 13(1), 31. https://doi.org/10.1186/s13073-021-00835-9
    • ", + "
  • Rentzsch, P., Witten, D., Cooper, G. M., Shendure, J., & Kircher, M. (2019). CADD: Predicting the deleteriousness of variants throughout the human genome. Nucleic Acids Research, 47(D1), D886–D894. https://doi.org/10.1093/nar/gky1016
    • ", + "
  • Pedersen, B. S., Layer, R. M., & Quinlan, A. R. (2016). Vcfanno: Fast, flexible annotation of genetic variants. Genome Biology, 17(1), 118. https://doi.org/10.1186/s13059-016-0973-5
    • ", + "
  • McLaren, W., Gil, L., Hunt, S. E., Riat, H. S., Ritchie, G. R. S., Thormann, A., Flicek, P., & Cunningham, F. (2016). The Ensembl Variant Effect Predictor. Genome Biology, 17(1), 122. https://doi.org/10.1186/s13059-016-0974-4
    • ", + "
  • Preste, R., Clima, R., & Attimonelli, M. (2019). Human mitochondrial variant annotation with HmtNote [Preprint]. Bioinformatics. https://doi.org/10.1101/600619
    • ", + "
  • Weissensteiner, H., Pacher, D., Kloss-Brandstätter, A., Forer, L., Specht, G., Bandelt, H.-J., Kronenberg, F., Salas, A., & Schönherr, S. (2016). HaploGrep 2: Mitochondrial haplogroup classification in the era of high-throughput sequencing. Nucleic Acids Research, 44(W1), W58–W63. https://doi.org/10.1093/nar/gkw233
    • ", + "
  • Magnusson, M., Hughes, T., Glabilloy, & Bitdeli Chef. (2018). genmod: Version 3.7.3 (3.7.3) [Computer software]. Zenodo. https://doi.org/10.5281/ZENODO.3841142
    • ", + ] + } + if (!params.skip_me_annotation) { + me_annotation_text = [ + "
  • McLaren, W., Gil, L., Hunt, S. E., Riat, H. S., Ritchie, G. R. S., Thormann, A., Flicek, P., & Cunningham, F. (2016). The Ensembl Variant Effect Predictor. Genome Biology, 17(1), 122. https://doi.org/10.1186/s13059-016-0974-4
    • ", + "
  • Eisfeldt, J., Vezzi, F., Olason, P., Nilsson, D., & Lindstrand, A. (2017). TIDDIT, an efficient and comprehensive structural variant caller for massive parallel sequencing data. F1000Research, 6, 664. https://doi.org/10.12688/f1000research.11168.2
    • ", + ] + } + qc_bam_text = [ + "
  • Broad Institute. (2023). Picard Tools. In Broad Institute, GitHub repository. http://broadinstitute.github.io/picard/
    • ", + "
  • Okonechnikov, K., Conesa, A., & García-Alcalde, F. (2016). Qualimap 2: Advanced multi-sample quality control for high-throughput sequencing data. Bioinformatics, 32(2), 292–294. https://doi.org/10.1093/bioinformatics/btv566
    • ", + "
  • Eisfeldt, J., Vezzi, F., Olason, P., Nilsson, D., & Lindstrand, A. (2017). TIDDIT, an efficient and comprehensive structural variant caller for massive parallel sequencing data. F1000Research, 6, 664. https://doi.org/10.12688/f1000research.11168.2
    • ", + "
  • Kent, W. J., Zweig, A. S., Barber, G., Hinrichs, A. S., & Karolchik, D. (2010). BigWig and BigBed: Enabling browsing of large distributed datasets. Bioinformatics, 26(17), 2204–2207. https://doi.org/10.1093/bioinformatics/btq351
    • ", + "
  • Pedersen, B. S., & Quinlan, A. R. (2018). Mosdepth: Quick coverage calculation for genomes and exomes. Bioinformatics, 34(5), 867–868. https://doi.org/10.1093/bioinformatics/btx699
    • ", + ] + me_calls_text = [ + "
  • Eisfeldt, J., Vezzi, F., Olason, P., Nilsson, D., & Lindstrand, A. (2017). TIDDIT, an efficient and comprehensive structural variant caller for massive parallel sequencing data. F1000Research, 6, 664. https://doi.org/10.12688/f1000research.11168.2
    • ", + "
  • Keane, T. M., Wong, K., & Adams, D. J. (2013). RetroSeq: Transposable element discovery from next-generation sequencing data. Bioinformatics, 29(3), 389–390. https://doi.org/10.1093/bioinformatics/bts697
    • ", + ] + preprocessing_text = [ + params.skip_fastqc ? "" : "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
    • ", + params.skip_fastp ? "" : "
  • Chen, S. (2023). Ultrafast one-pass FASTQ data preprocessing, quality control, and deduplication using fastp. iMeta, 2(2), e107. https://doi.org/10.1002/imt2.107
    • ", + ] + other_citation_text = [ + "
  • Danecek, P., Bonfield, J. K., Liddle, J., Marshall, J., Ohan, V., Pollard, M. O., Whitwham, A., Keane, T., McCarthy, S. A., Davies, R. M., & Li, H. (2021). Twelve years of SAMtools and BCFtools. GigaScience, 10(2), giab008. https://doi.org/10.1093/gigascience/giab008
    • ", + "
  • McKenna, A., Hanna, M., Banks, E., Sivachenko, A., Cibulskis, K., Kernytsky, A., Garimella, K., Altshuler, D., Gabriel, S., Daly, M., & DePristo, M. A. (2010). The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data. Genome Research, 20(9), 1297–1303. https://doi.org/10.1101/gr.107524.110
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: Summarize analysis results for multiple tools and samples in a single report. Bioinformatics, 32(19), 3047–3048. https://doi.org/10.1093/bioinformatics/btw354
    • ", + params.skip_peddy ? "" : "
  • Pedersen, B. S., & Quinlan, A. R. (2017). Who’s Who? Detecting and Resolving Sample Anomalies in Human DNA Sequencing Studies with Peddy. The American Journal of Human Genetics, 100(3), 406–413. https://doi.org/10.1016/j.ajhg.2017.01.017
    • ", + params.run_rtgvcfeval ? "
  • Cleary, J. G., Braithwaite, R., Gaastra, K., Hilbush, B. S., Inglis, S., Irvine, S. A., Jackson, A., Littin, R., Rathod, M., Ware, D., Zook, J. M., Trigg, L., & Vega, F. M. D. L. (2015). Comparing Variant Call Files for Performance Benchmarking of Next-Generation Sequencing Variant Calling Pipelines (p. 023754). bioRxiv. https://doi.org/10.1101/023754
    • " : "", + "
  • Li, H., Handsaker, B., Wysoker, A., Fennell, T., Ruan, J., Homer, N., Marth, G., Abecasis, G., Durbin, R., & 1000 Genome Project Data Processing Subgroup. (2009). The Sequence Alignment/Map format and SAMtools. Bioinformatics, 25(16), 2078–2079. https://doi.org/10.1093/bioinformatics/btp352
    • ", + "
  • Chen, X., Sanchis-Juan, A., French, C. E., Connell, A. J., Delon, I., Kingsbury, Z., Chawla, A., Halpern, A. L., Taft, R. J., Bentley, D. R., Butchbach, M. E. R., Raymond, F. L., & Eberle, M. A. (2020). Spinal muscular atrophy diagnosis and carrier screening from genome sequencing data. Genetics in Medicine, 22(5), 945–953. https://doi.org/10.1038/s41436-020-0754-0
    • ", + "
  • Li, H. (2011). Tabix: Fast retrieval of sequence features from generic TAB-delimited files. Bioinformatics, 27(5), 718–719. https://doi.org/10.1093/bioinformatics/btq671
    • ", + ] + + def concat_text = align_text + + variant_call_text + + repeat_call_text + + snv_annotation_text + + sv_annotation_text + + mt_annotation_text + + qc_bam_text + + me_calls_text + + me_annotation_text + + preprocessing_text + + other_citation_text + + def reference_text = concat_text.unique(false) { a, b -> a <=> b } - "" + return reference_text.join(' ').trim() } def methodsDescriptionText(mqc_methods_yaml) { @@ -257,14 +450,11 @@ def methodsDescriptionText(mqc_methods_yaml) { // Pipeline DOI meta["doi_text"] = meta.manifest_map.doi ? "(doi:     ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • Make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - // meta["tool_bibliography"] = toolBibliographyText() + meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + meta["tool_bibliography"] = toolBibliographyText() def methods_text = mqc_methods_yaml.text diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index dbc1fcb6..96debe46 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -685,7 +685,7 @@ workflow RAREDISEASE { // ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() - ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.fromPath("$projectDir/docs/images/nf-core-raredisease_logo_light.png", checkIfExists: true) summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) From 8b75be3a1f932a8c30001453dae67160249e2da8 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Wed, 13 Mar 2024 16:18:23 +0100 Subject: [PATCH 384/387] remove getGenomeAttributes from subworkflow --- .../local/utils_nfcore_raredisease_pipeline/main.nf | 12 +----------- 1 file changed, 1 insertion(+), 11 deletions(-) diff --git a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf index e2d24ec0..8ab5e642 100644 --- a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf @@ -178,17 +178,7 @@ def validateInputSamplesheet(input) { return [ metas[0], fastqs ] } -// -// Get attribute from genome config file e.g. fasta -// -def getGenomeAttribute(attribute) { - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - return params.genomes[ params.genome ][ attribute ] - } - } - return null -} + // // Replace spaces in vcf files with underscores // From f11aff261f3e49325c988600ae83467e93ea6aeb Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 14 Mar 2024 09:51:17 +0100 Subject: [PATCH 385/387] add peddy --- assets/multiqc_config.yml | 3 +++ 1 file changed, 3 insertions(+) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 174bb344..b3a8c6c8 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -21,6 +21,7 @@ run_modules: - qualimap - picard - mosdepth + - peddy - custom_content module_order: @@ -34,6 +35,8 @@ module_order: name: "Qualimap" - mosdepth: name: "Mosdepth" + - peddy: + name: "Peddy" extra_fn_clean_exts: - "_sorted_md" From f607faef103fe3d685db69618198c621673a9e18 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 14 Mar 2024 17:10:50 +0100 Subject: [PATCH 386/387] fix s3 issue --- modules/local/replace_spaces_in_vcfinfo.nf | 59 +++++++++++++++++++ subworkflows/local/annotate_mt_snvs.nf | 11 ++-- .../utils_nfcore_raredisease_pipeline/main.nf | 19 ------ 3 files changed, 63 insertions(+), 26 deletions(-) create mode 100644 modules/local/replace_spaces_in_vcfinfo.nf diff --git a/modules/local/replace_spaces_in_vcfinfo.nf b/modules/local/replace_spaces_in_vcfinfo.nf new file mode 100644 index 00000000..afd66029 --- /dev/null +++ b/modules/local/replace_spaces_in_vcfinfo.nf @@ -0,0 +1,59 @@ +process REPLACE_SPACES_IN_VCFINFO { + tag "$meta.id" + label 'process_single' + + conda "conda-forge::python=3.8.3" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/python:3.8.3' : + 'biocontainers/python:3.8.3' }" + + input: + tuple val(meta), path(input) + + output: + tuple val(meta), path("*_reformatted.vcf"), emit: vcf + path "versions.yml", emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + python3 < versions.yml + "${task.process}": + replace_spaces_in_vcfinfo: v1.0 + python: \$(python --version | sed 's/Python //g') + END_VERSIONS + """ + + stub: + """ + python3 < versions.yml + "${task.process}": + replace_spaces_in_vcfinfo: v1.0 + python: \$(python --version | sed 's/Python //g') + END_VERSIONS + """ +} diff --git a/subworkflows/local/annotate_mt_snvs.nf b/subworkflows/local/annotate_mt_snvs.nf index 393ac317..917893b4 100644 --- a/subworkflows/local/annotate_mt_snvs.nf +++ b/subworkflows/local/annotate_mt_snvs.nf @@ -2,7 +2,7 @@ // Annotate MT // -include { replaceSpacesInInfoColumn } from './utils_nfcore_raredisease_pipeline/main' +include { REPLACE_SPACES_IN_VCFINFO } from '../../modules/local/replace_spaces_in_vcfinfo' include { TABIX_TABIX as TABIX_TABIX_MT } from '../../modules/nf-core/tabix/tabix/main' include { ENSEMBLVEP_VEP as ENSEMBLVEP_MT } from '../../modules/nf-core/ensemblvep/vep/main' include { HAPLOGREP2_CLASSIFY as HAPLOGREP2_CLASSIFY_MT } from '../../modules/nf-core/haplogrep2/classify/main' @@ -83,12 +83,8 @@ workflow ANNOTATE_MT_SNVS { // HMTNOTE ANNOTATE HMTNOTE_ANNOTATE(ch_hmtnote_in) - HMTNOTE_ANNOTATE.out.vcf - .map{meta, vcf -> - return [meta, file(replaceSpacesInInfoColumn(vcf, vcf.parent.toString(), vcf.baseName))] - } - .set { ch_hmtnote_reformatted } - ZIP_TABIX_HMTNOTE(ch_hmtnote_reformatted) + REPLACE_SPACES_IN_VCFINFO(HMTNOTE_ANNOTATE.out.vcf) + ZIP_TABIX_HMTNOTE(REPLACE_SPACES_IN_VCFINFO.out.vcf) // Prepare output ch_vcf_out = ZIP_TABIX_HMTNOTE.out.gz_tbi.map{meta, vcf, tbi -> return [meta, vcf] } @@ -103,6 +99,7 @@ workflow ANNOTATE_MT_SNVS { ch_versions = ch_versions.mix(HMTNOTE_ANNOTATE.out.versions) ch_versions = ch_versions.mix(HAPLOGREP2_CLASSIFY_MT.out.versions) ch_versions = ch_versions.mix(ZIP_TABIX_HMTNOTE.out.versions) + ch_versions = ch_versions.mix(REPLACE_SPACES_IN_VCFINFO.out.versions) emit: haplog = HAPLOGREP2_CLASSIFY_MT.out.txt // channel: [ val(meta), path(txt) ] diff --git a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf index 8ab5e642..912c7ace 100644 --- a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf @@ -179,25 +179,6 @@ def validateInputSamplesheet(input) { return [ metas[0], fastqs ] } -// -// Replace spaces in vcf files with underscores -// -def replaceSpacesInInfoColumn(vcf_file, parent_dir, base_name) { - def outfile = new File(parent_dir + '/' + base_name + '_formatted.vcf') - def writer = outfile.newWriter() - vcf_file.eachLine { line -> - if (line.startsWith("#")) { - writer << line + "\n" - } else { - def split_str = line.tokenize("\t") - split_str[7] = split_str.getAt(7).replaceAll(" ","_") - writer << split_str.join("\t") + "\n" - } - } - writer.close() - return outfile -} - // // Exit pipeline if incorrect --genome key provided // From b7af9ff3e858f0d52331dc60a00957e49a5607ef Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 18 Mar 2024 10:32:34 +0100 Subject: [PATCH 387/387] review suggestions --- docs/images/raredisease_metromap_dark.pdf | Bin 199627 -> 199656 bytes docs/images/raredisease_metromap_dark.png | Bin 429743 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