diff --git a/README.md b/README.md index 14cc822..6d78831 100644 --- a/README.md +++ b/README.md @@ -20,6 +20,28 @@ In the [wiki](https://github.com/ncbi/BAMscale/wiki) pages we have more detailed 20190821: We recently added support for [RNA-seq](https://github.com/ncbi/BAMscale/wiki/Detailed-usage:-RNA-seq-coverage-tracks) data as well to create coverage tracks. The new method enables accurate representations of exon-intron boundaries (splicing). +## Manuals + +In the [wiki](https://github.com/ncbi/BAMscale/wiki) page we have more detailed tutorials for creating bigWig files and quantifying peaks: + +1. [OK-seq and RFD Track Generation](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-OKseq-RFD-(Replication-Fork-Directionality)-Track-Generation) +2. [Quantifying Peaks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Quantifying-Peak-Coverages-from-Multiple-BAM-Files#comparing-atac-seq-changes-induced-from-treatment) +3. [Generating Scaled Coverage Tracks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Generating-Scaled-Coverage-Tracks#preparing-input-data-for-bamscale) +4. [END-seq data](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Processing-END-seq-Data) +5. [Log2 Coverage Tracks for Replication Timing Data](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Replication-Timing-log2-Coverage-Ratio-from-Two-BAM-Files) +6. [Smoothening Function for Coverage Tracks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Smooth-Coverage-Tracks) + + +We also added a few R scripts that might be helpful for basic visualizations: + +1. [Segmenting replication timing bigwigs](https://github.com/ncbi/BAMscale/wiki/Replication-timing-BED-segments-from-bigwig) +2. [Identifying OK-seq strand switches](https://github.com/ncbi/BAMscale/wiki/Finding-OK-seq-strand-switched-from-the-RFD-track) + +For additional information, visit the [wiki](https://github.com/ncbi/BAMscale/wiki) page. + +For any other requests, or if you need help either open an issue, or feel free to email me: *pongorlorinc@gmail.com* + + ## Usage for the impatient These examples assume you have 4 processing threads, so we set '-t 4' for multithreading. @@ -54,26 +76,6 @@ These examples assume you have 4 processing threads, so we set '-t 4' for multit BAMscale scale -t 4 --operation endseq --bam -## Manuals - -In the [wiki](https://github.com/ncbi/BAMscale/wiki) page we have more detailed tutorials for creating bigWig files and quantifying peaks: - -1. [OK-seq and RFD Track Generation](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-OKseq-RFD-(Replication-Fork-Directionality)-Track-Generation) -2. [Quantifying Peaks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Quantifying-Peak-Coverages-from-Multiple-BAM-Files#comparing-atac-seq-changes-induced-from-treatment) -3. [Generating Scaled Coverage Tracks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Generating-Scaled-Coverage-Tracks#preparing-input-data-for-bamscale) -4. [END-seq data](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Processing-END-seq-Data) -5. [Log2 Coverage Tracks for Replication Timing Data](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Replication-Timing-log2-Coverage-Ratio-from-Two-BAM-Files) -6. [Smoothening Function for Coverage Tracks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Smooth-Coverage-Tracks) - - -We also added a few R scripts that might be helpful for basic visualizations: - -1. [Segmenting replication timing bigwigs](https://github.com/ncbi/BAMscale/wiki/Replication-timing-BED-segments-from-bigwig) -2. [Identifying OK-seq strand switches](https://github.com/ncbi/BAMscale/wiki/Finding-OK-seq-strand-switched-from-the-RFD-track) - -For additional information, visit the [wiki](https://github.com/ncbi/BAMscale/wiki) page. - -For any other requests, or if you need help either open an issue, or feel free to email me: *pongorlorinc@gmail.com* ## Reference