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pongorlorinc authored Aug 23, 2019
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20190821: We recently added support for [RNA-seq](https://github.com/ncbi/BAMscale/wiki/Detailed-usage:-RNA-seq-coverage-tracks) data as well to create coverage tracks. The new method enables accurate representations of exon-intron boundaries (splicing).

## Manuals

In the [wiki](https://github.com/ncbi/BAMscale/wiki) page we have more detailed tutorials for creating bigWig files and quantifying peaks:

1. [OK-seq and RFD Track Generation](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-OKseq-RFD-(Replication-Fork-Directionality)-Track-Generation)
2. [Quantifying Peaks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Quantifying-Peak-Coverages-from-Multiple-BAM-Files#comparing-atac-seq-changes-induced-from-treatment)
3. [Generating Scaled Coverage Tracks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Generating-Scaled-Coverage-Tracks#preparing-input-data-for-bamscale)
4. [END-seq data](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Processing-END-seq-Data)
5. [Log2 Coverage Tracks for Replication Timing Data](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Replication-Timing-log2-Coverage-Ratio-from-Two-BAM-Files)
6. [Smoothening Function for Coverage Tracks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Smooth-Coverage-Tracks)


We also added a few R scripts that might be helpful for basic visualizations:

1. [Segmenting replication timing bigwigs](https://github.com/ncbi/BAMscale/wiki/Replication-timing-BED-segments-from-bigwig)
2. [Identifying OK-seq strand switches](https://github.com/ncbi/BAMscale/wiki/Finding-OK-seq-strand-switched-from-the-RFD-track)

For additional information, visit the [wiki](https://github.com/ncbi/BAMscale/wiki) page.

For any other requests, or if you need help either open an issue, or feel free to email me: *[email protected]*


## Usage for the impatient

These examples assume you have 4 processing threads, so we set '-t 4' for multithreading.
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BAMscale scale -t 4 --operation endseq --bam <ENDseq.bam>

## Manuals

In the [wiki](https://github.com/ncbi/BAMscale/wiki) page we have more detailed tutorials for creating bigWig files and quantifying peaks:

1. [OK-seq and RFD Track Generation](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-OKseq-RFD-(Replication-Fork-Directionality)-Track-Generation)
2. [Quantifying Peaks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Quantifying-Peak-Coverages-from-Multiple-BAM-Files#comparing-atac-seq-changes-induced-from-treatment)
3. [Generating Scaled Coverage Tracks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Generating-Scaled-Coverage-Tracks#preparing-input-data-for-bamscale)
4. [END-seq data](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Processing-END-seq-Data)
5. [Log2 Coverage Tracks for Replication Timing Data](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Replication-Timing-log2-Coverage-Ratio-from-Two-BAM-Files)
6. [Smoothening Function for Coverage Tracks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Smooth-Coverage-Tracks)


We also added a few R scripts that might be helpful for basic visualizations:

1. [Segmenting replication timing bigwigs](https://github.com/ncbi/BAMscale/wiki/Replication-timing-BED-segments-from-bigwig)
2. [Identifying OK-seq strand switches](https://github.com/ncbi/BAMscale/wiki/Finding-OK-seq-strand-switched-from-the-RFD-track)

For additional information, visit the [wiki](https://github.com/ncbi/BAMscale/wiki) page.

For any other requests, or if you need help either open an issue, or feel free to email me: *[email protected]*

## Reference

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