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@@ -20,6 +20,28 @@ In the [wiki](https://github.com/ncbi/BAMscale/wiki) pages we have more detailed | |
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20190821: We recently added support for [RNA-seq](https://github.com/ncbi/BAMscale/wiki/Detailed-usage:-RNA-seq-coverage-tracks) data as well to create coverage tracks. The new method enables accurate representations of exon-intron boundaries (splicing). | ||
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## Manuals | ||
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In the [wiki](https://github.com/ncbi/BAMscale/wiki) page we have more detailed tutorials for creating bigWig files and quantifying peaks: | ||
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1. [OK-seq and RFD Track Generation](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-OKseq-RFD-(Replication-Fork-Directionality)-Track-Generation) | ||
2. [Quantifying Peaks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Quantifying-Peak-Coverages-from-Multiple-BAM-Files#comparing-atac-seq-changes-induced-from-treatment) | ||
3. [Generating Scaled Coverage Tracks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Generating-Scaled-Coverage-Tracks#preparing-input-data-for-bamscale) | ||
4. [END-seq data](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Processing-END-seq-Data) | ||
5. [Log2 Coverage Tracks for Replication Timing Data](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Replication-Timing-log2-Coverage-Ratio-from-Two-BAM-Files) | ||
6. [Smoothening Function for Coverage Tracks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Smooth-Coverage-Tracks) | ||
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We also added a few R scripts that might be helpful for basic visualizations: | ||
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1. [Segmenting replication timing bigwigs](https://github.com/ncbi/BAMscale/wiki/Replication-timing-BED-segments-from-bigwig) | ||
2. [Identifying OK-seq strand switches](https://github.com/ncbi/BAMscale/wiki/Finding-OK-seq-strand-switched-from-the-RFD-track) | ||
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For additional information, visit the [wiki](https://github.com/ncbi/BAMscale/wiki) page. | ||
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For any other requests, or if you need help either open an issue, or feel free to email me: *[email protected]* | ||
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## Usage for the impatient | ||
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These examples assume you have 4 processing threads, so we set '-t 4' for multithreading. | ||
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BAMscale scale -t 4 --operation endseq --bam <ENDseq.bam> | ||
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## Manuals | ||
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In the [wiki](https://github.com/ncbi/BAMscale/wiki) page we have more detailed tutorials for creating bigWig files and quantifying peaks: | ||
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1. [OK-seq and RFD Track Generation](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-OKseq-RFD-(Replication-Fork-Directionality)-Track-Generation) | ||
2. [Quantifying Peaks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Quantifying-Peak-Coverages-from-Multiple-BAM-Files#comparing-atac-seq-changes-induced-from-treatment) | ||
3. [Generating Scaled Coverage Tracks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Generating-Scaled-Coverage-Tracks#preparing-input-data-for-bamscale) | ||
4. [END-seq data](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Processing-END-seq-Data) | ||
5. [Log2 Coverage Tracks for Replication Timing Data](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Replication-Timing-log2-Coverage-Ratio-from-Two-BAM-Files) | ||
6. [Smoothening Function for Coverage Tracks](https://github.com/ncbi/BAMscale/wiki/Detailed-Use:-Smooth-Coverage-Tracks) | ||
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We also added a few R scripts that might be helpful for basic visualizations: | ||
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||
1. [Segmenting replication timing bigwigs](https://github.com/ncbi/BAMscale/wiki/Replication-timing-BED-segments-from-bigwig) | ||
2. [Identifying OK-seq strand switches](https://github.com/ncbi/BAMscale/wiki/Finding-OK-seq-strand-switched-from-the-RFD-track) | ||
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For additional information, visit the [wiki](https://github.com/ncbi/BAMscale/wiki) page. | ||
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For any other requests, or if you need help either open an issue, or feel free to email me: *[email protected]* | ||
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## Reference | ||
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