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There is documentation regarding how to use non-primary alignments in some of the modkit commands. However, as of now pileup does not aggregate non-primary alignments. It's not impossible by any means, but there are some sharp edges so I want to make sure it works correctly first. Are you looking for something like pore-c?
Dear @ALL
I think this question would be more appropriate here, so I referenced it here (nanoporetech/dorado#1089)
Thank you for your time.
Kind regards
Zheng zhuqing
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