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test rna_starsolo 2.7.8a #45

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jennaj opened this issue Jun 24, 2022 · 4 comments
Closed

test rna_starsolo 2.7.8a #45

jennaj opened this issue Jun 24, 2022 · 4 comments

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@jennaj
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jennaj commented Jun 24, 2022

Test for one weird error reported to bugs.

toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo/2.7.8a

@jennaj
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jennaj commented Jun 24, 2022

/run-tool-test tool_id=toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo/2.7.8a

@mvdbeek
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mvdbeek commented Jun 24, 2022

Results (powered by Planemo)

Summary

State Count
Total 8
Passed 6
Error 2
Failure 0
Skipped 0
Errored s
Passed s
  • ✅ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test tool test #1)

    Command Line:

    Exit Code:

    • 0

    Standard Error:

    • [bam_sort_core] merging from 0 files and 9 in-memory blocks...
      

    Standard Output:

    • Jun 24 23:28:30 ..... started STAR run
      Jun 24 23:28:33 ... starting to generate Genome files
      Jun 24 23:28:34 ..... processing annotations GTF
      Jun 24 23:28:52 ... starting to sort Suffix Array. This may take a long time...
      Jun 24 23:28:53 ... sorting Suffix Array chunks and saving them to disk...
      Jun 24 23:29:03 ... loading chunks from disk, packing SA...
      Jun 24 23:29:15 ... finished generating suffix array
      Jun 24 23:29:16 ... generating Suffix Array index
      Jun 24 23:29:16 ... completed Suffix Array index
      Jun 24 23:29:19 ..... inserting junctions into the genome indices
      Jun 24 23:29:20 ... writing Genome to disk ...
      Jun 24 23:29:22 ... writing Suffix Array to disk ...
      Jun 24 23:29:25 ... writing SAindex to disk
      Jun 24 23:29:26 ..... finished successfully
      Jun 24 23:29:32 ..... started STAR run
      Jun 24 23:29:51 ..... loading genome
      Jun 24 23:29:59 ..... started mapping
      Jun 24 23:30:46 ..... finished mapping
      Jun 24 23:30:50 ..... started Solo counting
      Jun 24 23:31:03 ..... finished Solo counting
      Jun 24 23:31:03 ..... finished successfully
      

    Job Parameters:

    • Job parameter Parameter value
      refGenomeSource {"__current_case__": 1, "geneSource": "history", "genomeFastaFiles": {"values": [{"id": 99483508, "src": "hda"}]}, "genomeSAindexNbases": "4", "sjdbGTFfile": {"values": [{"id": 99483360, "src": "hda"}]}, "sjdbOverhang": "100"}
      sc {"__current_case__": 0, "input_types": {"__current_case__": 0, "input1": {"values": [{"id": 99483368, "src": "hda"}]}, "input2": {"values": [{"id": 99483377, "src": "hda"}]}, "use": "repeat"}, "params": {"__current_case__": 1, "chemistry": "CR3"}, "soloBarcodeReadLength": "true", "soloCBmatchWLtype": "1MM_multi", "soloCBwhitelist": {"values": [{"id": 99483385, "src": "hda"}]}, "soloUMIdedup": "1MM_All", "solo_type": "CB_UMI_Simple"}
      solo {"filter": {"__current_case__": 3, "filter_type": "no_filter"}, "soloFeatures": "Gene", "soloOutFormatFeaturesGeneField3": "Gene Expression", "soloStrand": "Forward", "soloUMIfiltering": "-"}
      chromInfo "/cvmfs/data.galaxyproject.org/managed/len/ucsc/?.len"
      dbkey "?"
      __input_ext "input"
  • ✅ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test test against test.galaxyproject.org #2)

    Command Line:

    Exit Code:

    • 0

    Standard Error:

    • [bam_sort_core] merging from 0 files and 9 in-memory blocks...
      

    Standard Output:

    • Jun 24 23:28:34 ..... started STAR run
      Jun 24 23:28:37 ... starting to generate Genome files
      Jun 24 23:28:40 ..... processing annotations GTF
      Jun 24 23:28:56 ... starting to sort Suffix Array. This may take a long time...
      Jun 24 23:28:57 ... sorting Suffix Array chunks and saving them to disk...
      Jun 24 23:29:08 ... loading chunks from disk, packing SA...
      Jun 24 23:29:20 ... finished generating suffix array
      Jun 24 23:29:20 ... generating Suffix Array index
      Jun 24 23:29:20 ... completed Suffix Array index
      Jun 24 23:29:23 ..... inserting junctions into the genome indices
      Jun 24 23:29:24 ... writing Genome to disk ...
      Jun 24 23:29:26 ... writing Suffix Array to disk ...
      Jun 24 23:29:33 ... writing SAindex to disk
      Jun 24 23:29:35 ..... finished successfully
      Jun 24 23:29:38 ..... started STAR run
      Jun 24 23:29:59 ..... loading genome
      Jun 24 23:30:09 ..... started mapping
      Jun 24 23:30:56 ..... finished mapping
      Jun 24 23:31:03 ..... started Solo counting
      Jun 24 23:31:24 ..... finished Solo counting
      Jun 24 23:31:25 ..... finished successfully
      

    Job Parameters:

    • Job parameter Parameter value
      refGenomeSource {"__current_case__": 1, "geneSource": "history", "genomeFastaFiles": {"values": [{"id": 99483508, "src": "hda"}]}, "genomeSAindexNbases": "4", "sjdbGTFfile": {"values": [{"id": 99483360, "src": "hda"}]}, "sjdbOverhang": "100"}
      sc {"__current_case__": 0, "input_types": {"__current_case__": 0, "input1": {"values": [{"id": 99483368, "src": "hda"}]}, "input2": {"values": [{"id": 99483377, "src": "hda"}]}, "use": "repeat"}, "params": {"__current_case__": 2, "bccdna_mate": {"__current_case__": 0, "bc_location": "other_mate"}, "chemistry": "custom", "clipAdapterType": "Hamming", "soloAdapterMismatchesNmax": "1", "soloAdapterSequence": "-", "soloCBlen": "16", "soloCBstart": "1", "soloUMIlen": "12", "soloUMIstart": "17"}, "soloBarcodeReadLength": "true", "soloCBmatchWLtype": "1MM_multi", "soloCBwhitelist": {"values": [{"id": 99483385, "src": "hda"}]}, "soloUMIdedup": "1MM_All", "solo_type": "CB_UMI_Simple"}
      solo {"filter": {"__current_case__": 0, "filter_type": "cellranger2", "max_min_ratio": "10.0", "max_perc": "0.99", "n_expected": "3000"}, "soloFeatures": "Gene", "soloOutFormatFeaturesGeneField3": "Gene Expression", "soloStrand": "Forward", "soloUMIfiltering": "-"}
      chromInfo "/cvmfs/data.galaxyproject.org/managed/len/ucsc/?.len"
      dbkey "?"
      __input_ext "input"
  • ✅ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test Test Prokka 1.14.6+galaxy1 #3)

    Command Line:

    Exit Code:

    • 0

    Standard Error:

    • [bam_sort_core] merging from 0 files and 7 in-memory blocks...
      

    Standard Output:

    • Jun 24 23:22:52 ..... started STAR run
      Jun 24 23:22:52 ... starting to generate Genome files
      Jun 24 23:22:52 ..... processing annotations GTF
      Jun 24 23:22:52 ... starting to sort Suffix Array. This may take a long time...
      Jun 24 23:22:52 ... sorting Suffix Array chunks and saving them to disk...
      Jun 24 23:22:52 ... loading chunks from disk, packing SA...
      Jun 24 23:22:52 ... finished generating suffix array
      Jun 24 23:22:52 ... generating Suffix Array index
      Jun 24 23:22:52 ... completed Suffix Array index
      Jun 24 23:22:52 ..... inserting junctions into the genome indices
      Jun 24 23:22:52 ... writing Genome to disk ...
      Jun 24 23:22:52 ... writing Suffix Array to disk ...
      Jun 24 23:22:52 ... writing SAindex to disk
      Jun 24 23:22:52 ..... finished successfully
      Jun 24 23:22:52 ..... started STAR run
      Jun 24 23:22:52 ..... loading genome
      Jun 24 23:22:52 ..... started mapping
      Jun 24 23:23:46 ..... finished mapping
      Jun 24 23:23:46 ..... started Solo counting
      Jun 24 23:23:46 ..... finished Solo counting
      Jun 24 23:23:46 ..... finished successfully
      

    Job Parameters:

    • Job parameter Parameter value
      refGenomeSource {"__current_case__": 1, "geneSource": "history", "genomeFastaFiles": {"values": [{"id": 99483508, "src": "hda"}]}, "genomeSAindexNbases": "4", "sjdbGTFfile": {"values": [{"id": 99483360, "src": "hda"}]}, "sjdbOverhang": "100"}
      sc {"__current_case__": 0, "input_types": {"__current_case__": 0, "input1": {"values": [{"id": 99483368, "src": "hda"}, {"id": 99483368, "src": "hda"}, {"id": 99483368, "src": "hda"}]}, "input2": {"values": [{"id": 99483377, "src": "hda"}, {"id": 99483377, "src": "hda"}, {"id": 99483377, "src": "hda"}]}, "use": "repeat"}, "params": {"__current_case__": 1, "chemistry": "CR3"}, "soloBarcodeReadLength": "true", "soloCBmatchWLtype": "1MM_multi", "soloCBwhitelist": {"values": [{"id": 99483385, "src": "hda"}]}, "soloUMIdedup": "1MM_All", "solo_type": "CB_UMI_Simple"}
      solo {"filter": {"__current_case__": 0, "filter_type": "cellranger2", "max_min_ratio": "10.0", "max_perc": "0.99", "n_expected": "3000"}, "soloFeatures": "Gene", "soloOutFormatFeaturesGeneField3": "Gene Expression", "soloStrand": "Forward", "soloUMIfiltering": "-"}
      chromInfo "/cvmfs/data.galaxyproject.org/managed/len/ucsc/?.len"
      dbkey "?"
      __input_ext "input"
  • ✅ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test Test toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/3.5+galaxy1 #5)

    Command Line:

    Exit Code:

    • 0

    Standard Error:

    • [bam_sort_core] merging from 0 files and 7 in-memory blocks...
      

    Standard Output:

    • Jun 24 23:22:52 ..... started STAR run
      Jun 24 23:22:52 ... starting to generate Genome files
      Jun 24 23:22:52 ..... processing annotations GTF
      Jun 24 23:22:52 ... starting to sort Suffix Array. This may take a long time...
      Jun 24 23:22:52 ... sorting Suffix Array chunks and saving them to disk...
      Jun 24 23:22:52 ... loading chunks from disk, packing SA...
      Jun 24 23:22:52 ... finished generating suffix array
      Jun 24 23:22:52 ... generating Suffix Array index
      Jun 24 23:22:52 ... completed Suffix Array index
      Jun 24 23:22:52 ..... inserting junctions into the genome indices
      Jun 24 23:22:52 ... writing Genome to disk ...
      Jun 24 23:22:52 ... writing Suffix Array to disk ...
      Jun 24 23:22:52 ... writing SAindex to disk
      Jun 24 23:22:52 ..... finished successfully
      Jun 24 23:22:52 ..... started STAR run
      Jun 24 23:22:52 ..... loading genome
      Jun 24 23:22:52 ..... started mapping
      Jun 24 23:23:10 ..... finished mapping
      Jun 24 23:23:10 ..... started Solo counting
      Jun 24 23:23:10 ..... finished Solo counting
      Jun 24 23:23:10 ..... finished successfully
      

    Job Parameters:

    • Job parameter Parameter value
      refGenomeSource {"__current_case__": 1, "geneSource": "history", "genomeFastaFiles": {"values": [{"id": 99483508, "src": "hda"}]}, "genomeSAindexNbases": "4", "sjdbGTFfile": {"values": [{"id": 99483360, "src": "hda"}]}, "sjdbOverhang": "100"}
      sc {"__current_case__": 0, "input_types": {"__current_case__": 0, "input1": {"values": [{"id": 99483368, "src": "hda"}]}, "input2": {"values": [{"id": 99483377, "src": "hda"}]}, "use": "repeat"}, "params": {"__current_case__": 1, "chemistry": "CR3"}, "soloBarcodeReadLength": "true", "soloCBmatchWLtype": "1MM_multi_pseudocounts", "soloCBwhitelist": {"values": [{"id": 99483385, "src": "hda"}]}, "soloUMIdedup": "1MM_All", "solo_type": "CB_UMI_Simple"}
      solo {"filter": {"__current_case__": 2, "filter_type": "topcells", "n_cells": "5"}, "soloFeatures": "GeneFull", "soloOutFormatFeaturesGeneField3": "Dummy Text", "soloStrand": "Forward", "soloUMIfiltering": "MultiGeneUMI"}
      chromInfo "/cvmfs/data.galaxyproject.org/managed/len/ucsc/?.len"
      dbkey "?"
      __input_ext "input"
  • ✅ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test Test trinotate 3.2.2+galaxy0 #6)

    Command Line:

    Exit Code:

    • 0

    Standard Error:

    • [bam_sort_core] merging from 0 files and 9 in-memory blocks...
      

    Standard Output:

    • Jun 24 23:28:31 ..... started STAR run
      Jun 24 23:28:35 ... starting to generate Genome files
      Jun 24 23:28:35 ..... processing annotations GTF
      Jun 24 23:28:53 ... starting to sort Suffix Array. This may take a long time...
      Jun 24 23:28:53 ... sorting Suffix Array chunks and saving them to disk...
      Jun 24 23:29:02 ... loading chunks from disk, packing SA...
      Jun 24 23:29:15 ... finished generating suffix array
      Jun 24 23:29:15 ... generating Suffix Array index
      Jun 24 23:29:15 ... completed Suffix Array index
      Jun 24 23:29:18 ..... inserting junctions into the genome indices
      Jun 24 23:29:20 ... writing Genome to disk ...
      Jun 24 23:29:21 ... writing Suffix Array to disk ...
      Jun 24 23:29:24 ... writing SAindex to disk
      Jun 24 23:29:25 ..... finished successfully
      Jun 24 23:29:27 ..... started STAR run
      Jun 24 23:29:50 ..... loading genome
      Jun 24 23:29:59 ..... started mapping
      Jun 24 23:30:46 ..... finished mapping
      Jun 24 23:30:50 ..... started Solo counting
      Jun 24 23:31:10 ..... finished Solo counting
      Jun 24 23:31:11 ..... finished successfully
      

    Job Parameters:

    • Job parameter Parameter value
      solo {"filter": {"FDR": "0.01", "__current_case__": 1, "candMaxN": "20000", "filter_type": "emptydrops", "indMax": "90000", "indMin": "45000", "maxMinRatio": "10.0", "maxPercentile": "0.99", "nExpectedCells": "5", "simN": "10000", "umiMin": "500", "umiMinFracMedian": "0.01"}, "soloFeatures": "Gene", "soloOutFormatFeaturesGeneField3": "Gene Expression", "soloStrand": "Forward", "soloUMIfiltering": "-"}
      chromInfo "/cvmfs/data.galaxyproject.org/managed/len/ucsc/?.len"
      dbkey "?"
      __input_ext "input"
      refGenomeSource {"__current_case__": 1, "geneSource": "history", "genomeFastaFiles": {"values": [{"id": 99483508, "src": "hda"}]}, "genomeSAindexNbases": "4", "sjdbGTFfile": {"values": [{"id": 99483360, "src": "hda"}]}, "sjdbOverhang": "100"}
      sc {"__current_case__": 0, "input_types": {"__current_case__": 0, "input1": {"values": [{"id": 99483368, "src": "hda"}]}, "input2": {"values": [{"id": 99483377, "src": "hda"}]}, "use": "repeat"}, "params": {"__current_case__": 1, "chemistry": "CR3"}, "soloBarcodeReadLength": "true", "soloCBmatchWLtype": "1MM_multi", "soloCBwhitelist": {"values": [{"id": 99483385, "src": "hda"}]}, "soloUMIdedup": "1MM_All", "solo_type": "CB_UMI_Simple"}
  • ✅ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test Test pangolin 3.1.17+galaxy1 #7)

    Command Line:

    Exit Code:

    • 0

    Standard Error:

    • [bam_sort_core] merging from 0 files and 7 in-memory blocks...
      

    Standard Output:

    • Jun 24 23:24:50 ..... started STAR run
      Jun 24 23:24:50 ... starting to generate Genome files
      Jun 24 23:24:50 ..... processing annotations GTF
      Jun 24 23:24:50 ... starting to sort Suffix Array. This may take a long time...
      Jun 24 23:24:50 ... sorting Suffix Array chunks and saving them to disk...
      Jun 24 23:24:50 ... loading chunks from disk, packing SA...
      Jun 24 23:24:50 ... finished generating suffix array
      Jun 24 23:24:50 ... generating Suffix Array index
      Jun 24 23:24:50 ... completed Suffix Array index
      Jun 24 23:24:50 ..... inserting junctions into the genome indices
      Jun 24 23:24:50 ... writing Genome to disk ...
      Jun 24 23:24:50 ... writing Suffix Array to disk ...
      Jun 24 23:24:50 ... writing SAindex to disk
      Jun 24 23:24:50 ..... finished successfully
      Jun 24 23:24:50 ..... started STAR run
      Jun 24 23:24:50 ..... loading genome
      Jun 24 23:24:50 ..... started mapping
      Jun 24 23:24:50 ..... finished mapping
      Jun 24 23:24:50 ..... started Solo counting
      Jun 24 23:24:51 ..... finished Solo counting
      Jun 24 23:24:51 ..... finished successfully
      

    Job Parameters:

    • Job parameter Parameter value
      refGenomeSource {"__current_case__": 1, "geneSource": "history", "genomeFastaFiles": {"values": [{"id": 99483508, "src": "hda"}]}, "genomeSAindexNbases": "4", "sjdbGTFfile": {"values": [{"id": 99483360, "src": "hda"}]}, "sjdbOverhang": "100"}
      sc {"__current_case__": 1, "cb_whitelists": [{"__index__": 0, "cb_end_anchor": "2", "cb_end_anchor_pos": "-1", "cb_start_anchor": "0", "cb_start_anchor_pos": "0", "whitelist_file": {"values": [{"id": 99483376, "src": "hda"}]}}, {"__index__": 1, "cb_end_anchor": "3", "cb_end_anchor_pos": "8", "cb_start_anchor": "3", "cb_start_anchor_pos": "1", "whitelist_file": {"values": [{"id": 99483386, "src": "hda"}]}}], "clipAdapterType": "CellRanger4", "input_types": {"__current_case__": 0, "input1": {"values": [{"id": 99483362, "src": "hda"}]}, "input2": {"values": [{"id": 99483370, "src": "hda"}]}, "use": "repeat"}, "soloAdapterMismatchesNmax": "1", "soloAdapterSequence": "GAGTGATTGCTTGTGACGCCTT", "soloCBmatchWLtype": "1MM", "soloUMIdedup": "1MM_All", "solo_type": "CB_UMI_Complex", "umi_end_anchor": "3", "umi_end_anchor_pos": "14", "umi_start_anchor": "3", "umi_start_anchor_pos": "9"}
      solo {"filter": {"__current_case__": 0, "filter_type": "cellranger2", "max_min_ratio": "10.0", "max_perc": "0.99", "n_expected": "3000"}, "soloFeatures": "Gene", "soloOutFormatFeaturesGeneField3": "Gene Expression", "soloStrand": "Forward", "soloUMIfiltering": "-"}
      chromInfo "/cvmfs/data.galaxyproject.org/managed/len/ucsc/?.len"
      dbkey "?"
      __input_ext "input"

@jennaj
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Collaborator Author

jennaj commented Jun 27, 2022

/run-tool-test tool_id=toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo/2.7.8a

@mvdbeek
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mvdbeek commented Jun 27, 2022

Results (powered by Planemo)

Summary

State Count
Total 8
Passed 0
Error 8
Failure 0
Skipped 0
Errored s
  • ❌ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test tool test #1)

    Execution Problem:

    • Input staging problem: Expecting value: line 1 column 1 (char 0)
      
  • ❌ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test test against test.galaxyproject.org #2)

    Execution Problem:

    • Input staging problem: Expecting value: line 1 column 1 (char 0)
      
  • ❌ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test Test Prokka 1.14.6+galaxy1 #3)

    Execution Problem:

    • Input staging problem: Expecting value: line 1 column 1 (char 0)
      
  • ❌ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test Test roary 3.13.0+galaxy2 #4)

    Execution Problem:

    • Input staging problem: Expecting value: line 1 column 1 (char 0)
      
  • ❌ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test Test toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/3.5+galaxy1 #5)

    Execution Problem:

    • Input staging problem: Expecting value: line 1 column 1 (char 0)
      
  • ❌ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test Test trinotate 3.2.2+galaxy0 #6)

    Execution Problem:

    • Input staging problem: Expecting value: line 1 column 1 (char 0)
      
  • ❌ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test Test pangolin 3.1.17+galaxy1 #7)

    Execution Problem:

    • Input staging problem: Expecting value: line 1 column 1 (char 0)
      
  • ❌ toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo (Test Test SEACR 1.3+galaxy1 on usegalaxy.org #8)

    Execution Problem:

    • Input staging problem: Expecting value: line 1 column 1 (char 0)
      

@jennaj jennaj closed this as completed Jun 12, 2023
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