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Issue with small reference contigs #51

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Csam488 opened this issue Aug 7, 2022 · 0 comments
Open

Issue with small reference contigs #51

Csam488 opened this issue Aug 7, 2022 · 0 comments

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@Csam488
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Csam488 commented Aug 7, 2022

When a small (2-20kb) reference contig is used it appears that a significant portion of reads generated are the complete reference genome and have the exact same sequence (IGV trace attached for context). I have tested this with both provided pore models and various -e ,-f, and -s values. I unfortunately don't know if this is DeepSimulator functioning as intended but it's causing difficulty as I'm attempting to simulate sequencing of PCR products. Could this potentially be solved by introducing a maximum read length parameter?

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