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There are some classes of Augustus output genes that are a bit puzzling, such as the one below. The transcript starts with an intron and then the following (first) CDS fragment has a non-zero phase value, which goes against the GFF specification (in my understanding of it.) This needs to be checked and corrected for.
# start gene g856
NODE_4651_length_1024_cov_24.708984 AUGUSTUS gene 1 1068 0.91 + . g856
NODE_4651_length_1024_cov_24.708984 AUGUSTUS transcript 1 1068 0.91 + . g856.t1
NODE_4651_length_1024_cov_24.708984 AUGUSTUS intron 1 106 0.91 + . transcript_id "g856.t1"; gene_id "g856";
NODE_4651_length_1024_cov_24.708984 AUGUSTUS CDS 107 1068 0.91 + 2 transcript_id "g856.t1"; gene_id "g856";
NODE_4651_length_1024_cov_24.708984 AUGUSTUS stop_codon 1066 1068 . + 0 transcript_id "g856.t1"; gene_id "g856";
# protein sequence = [TQTSTAQSQAMDAESNTSTDPKNGDSQSALVQQLCQTVERLTNELSQARHEIQHLQERINTINSTTTPLSPLEFPTLQ
# ESQIRSTAFPDAPWNNPSKIQALKQPSIQRSEQRRMQREATAARFFQPPSENQGFKYLYIPTKARIPVGTIRTTFRKLGVNNARLLDIHYPARNTVAV
# LIHNDYEAEFVELLTRKNVHIRTDFTPFNGKILADPKYTSLPQEERDSIAIRLQKLRLSRALDYIRSPVKYAVARYFLDQEWISRTRYEEIMADRYNT
# KLTSIFDQTSQQQTTQDTFNDVSDNDLNMEAIDELPTGTSSPALH]
# Evidence for and against this transcript:
# % of transcript supported by hints (any source): 0
# CDS exons: 0/1
# CDS introns: 0/1
#5'UTR exons and introns: 0/0
#3'UTR exons and introns: 0/0
# hint groups fully obeyed: 0
# incompatible hint groups: 0
# end gene g856
The text was updated successfully, but these errors were encountered:
There are some classes of Augustus output genes that are a bit puzzling, such as the one below. The transcript starts with an intron and then the following (first) CDS fragment has a non-zero phase value, which goes against the GFF specification (in my understanding of it.) This needs to be checked and corrected for.
The text was updated successfully, but these errors were encountered: