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new_est_reads between samples #277

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liuxiangping123 opened this issue Jul 24, 2024 · 1 comment
Open

new_est_reads between samples #277

liuxiangping123 opened this issue Jul 24, 2024 · 1 comment

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@liuxiangping123
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Hello, when I was evaluating kraken2 results using bracken2, one of the samples was added_reads
That's orders of magnitude more. For example, there are 323 reads of g__Gabonibacter in kraken2, while 1249867 reads of braken2new_est_reads. There is only one species of g__Gabonibacter and 350 reads of new_est_reads. Other samples of g__Gabonibacter were evaluated in the hundreds.
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@liuxiangping123
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s__Gabonibacter massiliensis is the only species of g__Gabonibacter. All samples are evaluated at the species level in the same order of 10^2. I do not understand why the evaluation at the genus level is so different
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