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+ + + + + + + + diff --git a/DESCRIPTION b/DESCRIPTION deleted file mode 100644 index 970afb5..0000000 --- a/DESCRIPTION +++ /dev/null @@ -1,63 +0,0 @@ -Package: iUSEiSEE -Title: A Workshop to learn how to use the iSEE package -Version: 1.0.0 -Authors@R: - c( - person(given = "Federico", - family = "Marini", - role = c("aut", "cre"), - email = "marinif@uni-mainz.de", - comment = c(ORCID = "0000-0003-3252-7758")), - person(given = "Kevin", - family = "Rue-Albrecht", - role = c("aut"), - email = "kevinrue67@gmail.com", - comment = c(ORCID = "0000-0003-3899-3872")), - person(given = "Charlotte", - family = "Soneson", - role = c("aut"), - email = "charlottesoneson@gmail.com", - comment = c(ORCID = "0000-0003-3833-2169")), - person(given = "Aaron", - family = "Lun", - role = c("aut"), - email = "infinite.monkeys.with.keyboards@gmail.com", - comment = c(ORCID = "0000-0002-3564-4813")), - person(given = "Najla", - family = "Abassi", - role = c("aut"), - email = "abassi.nejla96@gmail.com", - comment = c(ORCID = "0000-0001-8357-0938")) - ) -Description: This package provides the material for a fully fledged one-day - workshop on the iSEE package. -License: MIT + file LICENSE -Encoding: UTF-8 -LazyData: true -Roxygen: list(markdown = TRUE) -RoxygenNote: 7.3.1 -Imports: - iSEE, - SingleCellExperiment, - scater -Suggests: - knitr, - rmarkdown, - pkgdown, - htmltools, - TENxPBMCData, - Seurat, - zellkonverter, - scran, - BiocSingular, - iSEEu, - iSEEde, - iSEEpathways, - iSEEhub, - iSEEindex, - iSEEfier, - yaml, - BiocStyle -URL: https://imbeimainz.github.io/iUSEiSEE/ -BugReports: https://github.com/imbeimainz/iUSEiSEE/issues -VignetteBuilder: knitr diff --git a/Dockerfile b/Dockerfile deleted file mode 100644 index 97c8357..0000000 --- a/Dockerfile +++ /dev/null @@ -1,9 +0,0 @@ -FROM bioconductor/bioconductor_docker:devel - -WORKDIR /home/rstudio - -COPY --chown=rstudio:rstudio . /home/rstudio/ - -RUN Rscript -e "options(repos = c(CRAN = 'https://cran.r-project.org')); BiocManager::install(ask=FALSE)" - -RUN Rscript -e "options(repos = c(CRAN = 'https://cran.r-project.org')); devtools::install('.', dependencies=TRUE, build_vignettes=TRUE, repos = BiocManager::repositories())" diff --git a/LICENSE b/LICENSE deleted file mode 100644 index 3e25e70..0000000 --- a/LICENSE +++ /dev/null @@ -1,2 +0,0 @@ -YEAR: 2024 -COPYRIGHT HOLDER: iUSEiSEE authors diff --git a/LICENSE-text.html b/LICENSE-text.html new file mode 100644 index 0000000..0ec2e46 --- /dev/null +++ b/LICENSE-text.html @@ -0,0 +1,98 @@ + +License • iUSEiSEE + + +
+
+ + + +
+
+
+

License

+
+ +
YEAR: 2024
+COPYRIGHT HOLDER: iUSEiSEE authors
+
+ +
+ + + +
+ + + +
+ + + + + + + + diff --git a/LICENSE.html b/LICENSE.html new file mode 100644 index 0000000..eadc377 --- /dev/null +++ b/LICENSE.html @@ -0,0 +1,102 @@ + +MIT License • iUSEiSEE + + +
+
+ + + +
+
+
+

MIT License

+
+ +
+ +

Copyright (c) 2024 iUSEiSEE authors

+

Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the “Software”), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:

+

The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software.

+

THE SOFTWARE IS PROVIDED “AS IS”, WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.

+
+ +
+ + + +
+ + + +
+ + + + + + + + diff --git a/LICENSE.md b/LICENSE.md deleted file mode 100644 index d73ec9d..0000000 --- a/LICENSE.md +++ /dev/null @@ -1,21 +0,0 @@ -# MIT License - -Copyright (c) 2024 iUSEiSEE authors - -Permission is hereby granted, free of charge, to any person obtaining a copy -of this software and associated documentation files (the "Software"), to deal -in the Software without restriction, including without limitation the rights -to use, copy, modify, merge, publish, distribute, sublicense, and/or sell -copies of the Software, and to permit persons to whom the Software is -furnished to do so, subject to the following conditions: - -The above copyright notice and this permission notice shall be included in all -copies or substantial portions of the Software. - -THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR -IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, -FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE -AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER -LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, -OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE -SOFTWARE. diff --git a/NAMESPACE b/NAMESPACE deleted file mode 100644 index b4095ae..0000000 --- a/NAMESPACE +++ /dev/null @@ -1,5 +0,0 @@ -# Generated by roxygen2: do not edit by hand - -importFrom(SingleCellExperiment,SingleCellExperiment) -importFrom(iSEE,iSEE) -importFrom(scater,plotTSNE) diff --git a/R/iUSEiSEE-pkg.R b/R/iUSEiSEE-pkg.R deleted file mode 100644 index c9fcd17..0000000 --- a/R/iUSEiSEE-pkg.R +++ /dev/null @@ -1,10 +0,0 @@ -#' iUSEiSEE: Learning to use the package iSEE -#' -#' @importFrom iSEE iSEE -#' @importFrom SingleCellExperiment SingleCellExperiment -#' @importFrom scater plotTSNE -#' -#' @name iUSEiSEE-pkg -#' @docType package -#' @keywords internal -"_PACKAGE" diff --git a/README.md b/README.md deleted file mode 100644 index f330323..0000000 --- a/README.md +++ /dev/null @@ -1,37 +0,0 @@ - - -# iUSEiSEE - -The `iSEE` package is a flexible, powerful and extendible application to explore any dataset stored in SummarizedExperiment objects, including single cell and spatially resolved data. - -`iSEE` enables a multitude of panels to gain in-depth insight in your data; it does so combining interactivity and reproducibility, and providing an ideal companion to CELLxGENE for deeper dives into data. - - -## How to install - -``` -library("remotes") -remotes::install_github("imbeimainz/iUSEiSEE", - dependencies = TRUE, - build_vignettes = TRUE) -``` - -## How to run this workshop - -- Open the `iUSEiSEE.Rproj` project file -- Navigate to the `vignettes` folder -- Make sure the datasets are included in the `datasets` subfolder (if using Seurat or AnnData) -- Open the individual Rmarkdown files and execute their content live! - -## Schedule - -* Short presentation, `iSEE`: Harry met Sally for interactive and reproducible data exploration -* Installation of the dependencies & tech check -* Retrieval of the data + Inspecting the processing steps so far -* Overview on iSEE: panel by panel, learning Data + Visual + Selection parameters -* Hands on with the full workshop -* Going through the iSEE cookbook: "What if all I need is some recipes?" -* Latest developments in the iSEE universe: iSEE for DE and pathways -* Got more than just one dataset? iSEEhub and iSEEindex -* iSEEfier: starting things up in a breeze -* Got some own data? Some space for additional questions and setting up iSEE on that diff --git a/_pkgdown.yml b/_pkgdown.yml deleted file mode 100644 index be5cd7c..0000000 --- a/_pkgdown.yml +++ /dev/null @@ -1,42 +0,0 @@ -url: https://imbeimainz.github.io/iUSEiSEE - -template: - params: - bootswatch: cosmo - #ganalytics: UA-99999999-9 - -home: - title: "iUSEiSEE" - type: inverse - - -navbar: - right: - - icon: fa-github - href: https://github.com/imbeimainz/iUSEiSEE - components: - home: - icon: fas fa-home fa-lg - href: index.html - reference: - text: Reference - href: reference/index.html - github: - icon: fab fa-github fa-lg - href: https://github.com/imbeimainz/iUSEiSEE/ - articles: - text: Workshop sessions - menu: - - text: 00. About this workshop - href: articles/about_this_workshop_00.html - - text: 01. iUSEiSEE in a glance - href: articles/iUSEiSEE_01.html - - text: 02. An overview on iSEE - href: articles/iSEE_overview_02.html - - text: 03. The iSEE cookbook - href: articles/iSEE_quickrecipes_03.html - - text: 04. Bonus iSEE content - href: articles/bonus_content_04.html - - - diff --git a/pkgdown/favicon/apple-touch-icon-120x120.png b/apple-touch-icon-120x120.png similarity index 100% rename from pkgdown/favicon/apple-touch-icon-120x120.png rename to apple-touch-icon-120x120.png diff --git a/pkgdown/favicon/apple-touch-icon-152x152.png b/apple-touch-icon-152x152.png similarity index 100% rename from pkgdown/favicon/apple-touch-icon-152x152.png rename to apple-touch-icon-152x152.png diff --git a/pkgdown/favicon/apple-touch-icon-180x180.png b/apple-touch-icon-180x180.png similarity index 100% rename from pkgdown/favicon/apple-touch-icon-180x180.png rename to apple-touch-icon-180x180.png diff --git a/pkgdown/favicon/apple-touch-icon-60x60.png b/apple-touch-icon-60x60.png similarity index 100% rename from pkgdown/favicon/apple-touch-icon-60x60.png rename to apple-touch-icon-60x60.png diff --git a/pkgdown/favicon/apple-touch-icon-76x76.png b/apple-touch-icon-76x76.png similarity index 100% rename from pkgdown/favicon/apple-touch-icon-76x76.png rename to apple-touch-icon-76x76.png diff --git a/pkgdown/favicon/apple-touch-icon.png b/apple-touch-icon.png similarity index 100% rename from pkgdown/favicon/apple-touch-icon.png rename to apple-touch-icon.png diff --git a/articles/about_this_workshop_00.html b/articles/about_this_workshop_00.html new file mode 100644 index 0000000..7b558dd --- /dev/null +++ b/articles/about_this_workshop_00.html @@ -0,0 +1,303 @@ + + + + + + + +iUSEiSEE - About this workshop • iUSEiSEE + + + + + + + + + + + + + + + + + + + +
+
+ + + + +
+
+
+

iUSEiSEE - About this workshop

+

Federico +Marini1, +Najla Abassi2

+
+Collection of material developed by the iSEE core dev team
(Kevin +Rue-Albrecht, Federico Marini, Charlotte Soneson, Aaron Lun)

+ + + Source: vignettes/about_this_workshop_00.Rmd + + +
+ + + +

+
+

Description +

+

The iSEE package (Rue-Albrecht et al. +2018) is a flexible, powerful and extendible application to +explore any dataset stored in SummarizedExperiment objects, including +single cell, spatially resolved data, but also bulk data +(transcriptomics and proteomics being just two examples of what can +efficiently fit in there!).

+

iSEE enables a multitude of panels to gain in-depth insight in your +data; it does so combining interactivity and reproducibility, and +providing an ideal companion to other tools (such as CELLxGENE) for +deeper dives into data.

+

This package provides a complete overview of the functionality of the +iSEE +Bioconductor package, and is intended as some kind of +“masterclass-level” workshop - indeed, the material is enough to cover a +full-day workshop on iSEE.

+
+
+

Pre-requisites +

+
    +
  • Familiarity with bulk or single-cell sequencing data (ideally +scRNA-seq)
    • +
  • Familiarity with the SummarizedExperiment/SingleCellExperiment +class, or alternatively the Seurat or AnnData frameworks - basically, +any of the widely used ecosystem for working with/storing/processing +single cell data
    • +
  • Familiarity with single-cell data visualizations and +representations
    • +
+
+
+

Participation +

+

Participants will be able (and heartily invited) to follow along in +the live coding parts of the workshop. This is very much intended as a +hands-on learning resource.

+

Given the hands-on nature of this, this is going to be a BYOD (Bring +Your Own Device) event - if cloud-based setups are provided, this +information will be passed on in advance to the participants. +Nonetheless, we value the side effect of participants installing a +working environment as a part of the learning objectives.

+

Participants will (likely) use a set of stickers to indicate their +progress throughout the material, to be attached onto the laptop lid. In +brief:

+
    +
  • Yellow sticker: “I am working on the task”
    • +
  • Green sticker: “I am done with the task”
    • +
  • Red sticker: “I am stuck on this, could you please come help +me?”
    • +
+

We do stress the importance of working together on such tasks, +therefore participants are very encouraged to talk to their neighbors +during the task sessions!

+
+
+

+R / Bioconductor packages used +

+ +
+
+

Outline of the course +

+

After a short presentation, we will start going through the material +in the vignettes folder:

+
    +
  • +iUSEiSEE - a tl;dr version of the +whole, which can be used as a compact summary of the content
    • +
  • +Overview on iSEE - a detailed +overview on iSEE and its functionality
    • +
  • +The iSEE cookbook - a set of +self-contained recipes with simple-to-advanced tasks one can complete +within iSEE
    • +
  • +Bonus advanced content around +iSEE - some additional content, focused on some advanced or more +specific tasks to carry out with iSEE
    • +
+
+
+

Workshop goals and objectives +

+
+

Learning goals +

+
    +
  • Outline the features of iSEE, built upon the RStudio Shiny +framework
    • +
  • Utilize interactive GUI components and layouts to efficiently +extract information from biological data sets
    • +
  • Describe how to construct interactive apps and custom panels
    • +
  • Demonstrate how these package can smoothly be integrated into and +enhance existing workflows for the datasets at hand
    • +
+
+
+

Learning objectives +

+
    +
  • Set up a local environment for running iSEE apps
    • +
  • Interact with components of the iSEE user interface to visually +inspect and discuss various data sets
    • +
  • Identify and locate configurable aspects of iSEE apps
    • +
  • Practice interactive visualization over a single-cell RNA-sequencing +workflow
    • +
  • Imagine use cases and future developments for interactive +visualization as part of computational workflows
    • +
  • Design and create iSEE instances for collections of datasets
    • +
+
+
+
+

References +

+
+
+Rue-Albrecht, Kevin, Federico Marini, Charlotte Soneson, and Aaron T L +Lun. 2018. “iSEE: Interactive +SummarizedExperiment Explorer.” F1000Res. 7 +(June). +
+
+
+
+
+
    +

  1. ↩︎

    2. +

  2. ↩︎

    3. +
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+ + + +
+ + + + +
+ + + + + + + + diff --git a/articles/bonus_content_04.html b/articles/bonus_content_04.html new file mode 100644 index 0000000..f97d84d --- /dev/null +++ b/articles/bonus_content_04.html @@ -0,0 +1,655 @@ + + + + + + + +iUSEiSEE - Bonus content • iUSEiSEE + + + + + + + + + + + + + + + + + + + +
+
+ + + + +
+ +
+
+
+

iUSEiSEE - Bonus content

+

Federico +Marini1, +Najla Abassi2

+
+Collection of material developed by the iSEE core dev team
(Kevin +Rue-Albrecht, Federico Marini, Charlotte Soneson, Aaron Lun)

+ + + Source: vignettes/bonus_content_04.Rmd + + +
+ + + +

+
+

Moar iSEE things +

+

This vignette contains some examples on even more +iSEE-things (Rue-Albrecht et al. +2018).

+

We will use the following packages throughout its content (make sure +to have them installed if you want to run it interactively)

+ +
+
+

iSEE a challenge: let’s reproduce any figure! +

+

We’d like to open up a challenge, with some simple rules:

+
    +
  • You search a nice graphical representation of data
    • +
  • We try to reproduce this within iSEE - as far as this +is possible!
    • +
+

Let’s suggest a couple of figure/figure panels!
+Enter a couple of suggestions in this GSheet: https://docs.google.com/spreadsheets/d/1poE713rXqzfNdPcKAPN2AxJYXhaxy8GwgAjRsk_UiK8/edit?usp=sharing

+
+
+

iSEEde and iSEEpathways +

+

iSEEde and iSEEpathways: ideal companions for exploring DE +results

+

We first load the processed macrophage data (derived +from the work of (Alasoo et al. 2018)) - +on this, we already ran the workflow of DESeq2 (Love, Huber, and Anders 2014) to identify the +differentially expressed genes.

+
+macrophage_location <- system.file("datasets", "sce_macrophage_readytouse.RDS",
+  package = "iUSEiSEE"
+)
+macrophage_location
+#> [1] "/Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/library/iUSEiSEE/datasets/sce_macrophage_readytouse.RDS"
+
+sce_macrophage <- readRDS(macrophage_location)
+
+library("iSEE")
+library("iSEEde")
+library("iSEEpathways")
+

iSEEde and +iSEEpathways +are two new Bioconductor packages that provide iSEE panels +specifically aimed towards exploration of differential expression and +pathway analysis results. More precisely, iSEEde +provides the VolcanoPlot, MAPlot, +LogFCLogFCPlot and DETable panels. These +panels can be configured to extract data that was added via the +embedContrastResults() function above. Let’s look at an +example:

+
+app <- iSEE(sce_macrophage, initial = list(
+  DETable(
+    ContrastName = "IFNgTRUE.SL1344TRUE.DESeq2",
+    HiddenColumns = c("baseMean", "lfcSE", "stat")
+  ),
+  VolcanoPlot(ContrastName = "IFNgTRUE.SL1344TRUE.DESeq2"),
+  MAPlot(ContrastName = "IFNgTRUE.SL1344TRUE.DESeq2")
+))
+

+ + + + + +

Note how it is easy to switch to a different contrast in any of the +panels.

+
+app <- iSEE(sce_macrophage, initial = list(
+  iSEEde::DETable(
+    ContrastName = "IFNgTRUE.SL1344TRUE.DESeq2",
+    HiddenColumns = c("baseMean", "lfcSE", "stat")
+  ),
+  iSEEde::VolcanoPlot(ContrastName = "IFNgTRUE.SL1344TRUE.DESeq2"),
+  iSEEde::MAPlot(ContrastName = "IFNgTRUE.SL1344TRUE.DESeq2"),
+  PathwaysTable(
+    ResultName = "IFNgTRUE.SL1344TRUE.limma.fgsea",
+    Selected = "GO:0046324"
+  ),
+  ComplexHeatmapPlot(
+    RowSelectionSource = "PathwaysTable1",
+    CustomRows = FALSE, ColumnData = "condition_name",
+    ClusterRows = TRUE, Assay = "vst"
+  ),
+  FgseaEnrichmentPlot(
+    ResultName = "IFNgTRUE.SL1344TRUE.limma.fgsea",
+    PathwayId = "GO:0046324"
+  )
+))
+

+ + + + + + + + +
+
+

iSEEfier +

+

Let’s say we are interested in visualizing the expression of a list +of specific marker genes in one view, or maybe we created different +initial states separately, but would like to visualize them in the same +instance. As we previously learned, we can do a lot of these tasks by +running the command:

+
+# don't run
+iSEE(sce)
+

Then, add/remove the panels to our need. This can involve multiple +steps (selecting the gene of interest, color by a specific +colData…), or probably even write multiple lines of code. +For that we can use the iSEEfier +package, which streamlines the process of starting (or if you will, +firing up) an iSEE instance with a small chunk of code, +avoiding the tedious way of setting up every iSEE panel +individually.

+

In this section, we will illustrate a simple example of how to use +iSEEfier. +We will use the same pbmc3k data we worked with during this +workshop.

+

We start by loading the data:

+
+library("iSEEfier")
+
+# import data
+sce <- readRDS(
+  file = system.file("datasets", "sce_pbmc3k.RDS", package = "iUSEiSEE")
+)
+
+sce
+#> class: SingleCellExperiment 
+#> dim: 32738 2643 
+#> metadata(0):
+#> assays(2): counts logcounts
+#> rownames(32738): MIR1302-10 FAM138A ... AC002321.2 AC002321.1
+#> rowData names(19): ENSEMBL_ID Symbol_TENx ... FDR_cluster11
+#>   FDR_cluster12
+#> colnames(2643): Cell1 Cell2 ... Cell2699 Cell2700
+#> colData names(24): Sample Barcode ... labels_ont cell_ontology_labels
+#> reducedDimNames(3): PCA TSNE UMAP
+#> mainExpName: NULL
+#> altExpNames(0):
+

For example, we can be interested in visualizing the expression of +GZMB, TGFB, and CD28 genes all at once. We +start by providing a couple of parameters:

+
+# define the list of genes
+feature_list_1 <- c("GZMB", "TGFB1", "CD28")
+# define the cluster/cell type 
+cluster_1 <- "labels_main"
+

Now we can pass these parameters into iSEEinit() to +create a customized initial configuration:

+
+# create an initial state with iSEEinit
+initial_1 <- iSEEinit(sce,
+                      features = feature_list_1,
+                      clusters = cluster_1,
+                      add_markdown_panel = TRUE)
+

The rest can be as easy as passing this initial to the +iSEE() call:

+
+app <- iSEE(sce, initial = initial_1)
+

This is how it would look like:

+

+

While we are visualizing the expression of these genes, we might want +to take some notes (gene X is more expressed in a certain cell +type/cluster than some others, maybe we are trying to annotate the cells +ourselves if the annotation wasn’t available…).
+For this, we used the argument add_markdown_panel = TRUE. +It will display a MarkdownBoard panel where we can note our +observations without leaving the app.

+

We can also use the argument add_dynamicTable_panel=TRUE +to add another custom panel to display the maker genes of certain +cluster/cell type.

+
+feature_list_2 <- c("CD74", "CD79B")
+initial_2 <- iSEEinit(sce,
+                      features = feature_list_2,
+                      clusters = cluster_1,
+                      add_markdown_panel = TRUE,
+                      add_dynamicTable_panel = TRUE)
+
+app <- iSEE(sce, initial = initial_2)
+

We can check the initial’s content, or how the included panels are +linked between each other without running the app with +view_initial_tiles() and +view_initial_network():

+
+# display a graphical representation of the initial configuration, where the panels are identified by their corresponding colors
+view_initial_tiles(initial = initial_1)
+

+
+# display a network visualization for the panels
+view_initial_network(initial_1, plot_format = "igraph")
+

+
#> IGRAPH 2f97daf DN-- 14 4 -- 
+#> + attr: name (v/c), color (v/c)
+#> + edges from 2f97daf (vertex names):
+#> [1] ReducedDimensionPlot1->ColumnDataPlot1  
+#> [2] ReducedDimensionPlot2->ColumnDataPlot1  
+#> [3] ReducedDimensionPlot3->ColumnDataPlot1  
+#> [4] ReducedDimensionPlot4->FeatureAssayPlot4
+

Another alternative for network visualization would use the +interactive widget provided by visNetwork:

+
+view_initial_network(initial_1, plot_format = "visNetwork")
+#> IGRAPH 1c615d0 DN-- 14 4 -- 
+#> + attr: name (v/c), color (v/c)
+#> + edges from 1c615d0 (vertex names):
+#> [1] ReducedDimensionPlot1->ColumnDataPlot1  
+#> [2] ReducedDimensionPlot2->ColumnDataPlot1  
+#> [3] ReducedDimensionPlot3->ColumnDataPlot1  
+#> [4] ReducedDimensionPlot4->FeatureAssayPlot4
+

It is also possible to combine multiple initials into one:

+
+merged_config <- glue_initials(initial_1, initial_2)
+#> Merging together 2 `initial` configuration objects...
+#> Combining sets of 14, 14 different panels.
+#> 
+#> Dropping 2 of the original list of 28 (detected as duplicated entries)
+#> 
+#> Some names of the panels were specified by the same name, but this situation can be handled at runtime by iSEE
+#> (This is just a non-critical message)
+#> 
+#> Returning an `initial` configuration including 26 different panels. Enjoy!
+#> If you want to obtain a preview of the panels configuration, you can call `view_initial_tiles()` on the output of this function
+# check out the content of merged_config
+view_initial_tiles(initial = merged_config)
+

+

?iSEEfier is always your friend whenever you need +further documentation on the package/a certain function and how to use +it.

+
+
+

Multi-sample, multi-condition datasets: how to let iSEE shine +

+

We will take another dataset for this, from a recent work of Bartneck +and colleagues ((Bartneck et al. +2023)).

+

You can find the dataset in the sce_merged_allsets.RDS +object - let’s see together how we can access that –> https://seafile.rlp.net/d/c7f1335a6248427d97da/.

+

Once the data is available locally, we load it in the R session and +simply start iSEE on it.

+
+library("iSEE")
+sce_bartneck <- readRDS("path/to/sce_merged_allsets.RDS")
+iSEE(sce_bartneck)
+

For more about this work, please refer to the original +publication.

+
+
+

iSEEhub & iSEEindex +

+
+

iSEEhub: iSEEing the ExperimentHub datasets +

+

The iSEEhub +package provides a custom landing page for an iSEE +application interfacing with the Bioconductor ExperimentHub. +The landing page allows users to browse the ExperimentHub, select a data +set, download and cache it, and import it directly into an iSEE app.

+
+library("iSEE")
+library("iSEEhub")
+#> Loading required package: ExperimentHub
+#> Loading required package: AnnotationHub
+#> Loading required package: BiocFileCache
+#> Loading required package: dbplyr
+#> 
+#> Attaching package: 'AnnotationHub'
+#> The following object is masked from 'package:Biobase':
+#> 
+#>     cache
+
+ehub <- ExperimentHub()
+app <- iSEEhub(ehub)
+

+ + + +
+
+

iSEEindex: one instance of iSEE to explore them +all +

+

iSEEindex +provides an interface to any collection of data sets +within a single iSEE web-application.
+The main functionality of this package is to define a custom landing +page allowing app maintainers to list a custom collection of data sets +that users can select from and directly load objects into an iSEE web +application. To see how to configure such an app, we will create a small +example:

+
+library("iSEE")
+library("iSEEindex")
+
+bfc <- BiocFileCache(cache = tempdir())
+
+dataset_fun <- function() {
+  x <- yaml::read_yaml(system.file(package = "iSEEindex", "example.yaml"))
+  x$datasets
+}
+initial_fun <- function() {
+  x <- yaml::read_yaml(system.file(package = "iSEEindex", "example.yaml"))
+  x$initial
+}
+app <- iSEEindex(bfc, dataset_fun, initial_fun)
+

+

A more elaborate example (referring to the work in (Rigby et al. 2023)) is available at https://rehwinkellab.shinyapps.io/ifnresource/. +The source can be found at https://github.com/kevinrue/IFNresource.

+ + + + + +

Potential use cases can include:

+
    +
  • An app to present and explore the different datasets in your next +publication
    • +
  • An app to explore collection of datasets collaboratively, in +consortium-like initiatives
    • +
  • An app to mirror and enhance the content of e.g. the cellxgene data +portal
    • +
  • Got any ideas on how to use iSEE for such deployments?
    • +
+
+
+
+

Tours: help and storytelling +

+

Tours can be an essential tool to satisfy two needs:

+
    +
  • Helping users to navigate the UI
    • +
  • Telling a story on an existing configuration
    • +
+

A simple example can be demonstrated with this configuration:

+
+sce_location <- system.file("datasets", "sce_pbmc3k.RDS", package = "iUSEiSEE")
+sce_location
+#> [1] "/Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/library/iUSEiSEE/datasets/sce_pbmc3k.RDS"
+sce <- readRDS(sce_location)
+
+initial_for_tour <- list(
+  ReducedDimensionPlot(PanelWidth = 3L), 
+  RowDataTable(PanelWidth = 3L),
+  FeatureAssayPlot(PanelWidth = 3L),
+  ComplexHeatmapPlot(PanelWidth = 3L)
+)
+

This next chunk defines the steps of the tour, +specified by an anchoring point (element) and the content +of that step (intro).

+
+tour <- data.frame(
+  element = c(
+    "#Welcome",
+    "#ReducedDimensionPlot1",
+    "#RowDataTable1",
+    "#ComplexHeatmapPlot1",
+    "#FeatureAssayPlot1",
+    "#ReducedDimensionPlot1",
+    "#Conclusion"),
+  intro = c(
+    "Welcome to this tour!",
+    "This is the a reduced dimension plot",
+    "and this is a table",
+    "Why not a heatmap?",
+    "... and now we look at one individual feature.",
+    "Back to the a reduced dimension plot...",
+    "Thank you for taking this tour!"),
+  stringsAsFactors = FALSE
+)
+
+app <- iSEE(sce, initial = initial_for_tour, tour = tour)
+

+
+
+

Session info +

+
+sessionInfo()
+#> R version 4.4.0 (2024-04-24)
+#> Platform: x86_64-apple-darwin20
+#> Running under: macOS Monterey 12.7.1
+#> 
+#> Matrix products: default
+#> BLAS:   /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRblas.0.dylib 
+#> LAPACK: /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRlapack.dylib;  LAPACK version 3.12.0
+#> 
+#> locale:
+#> [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
+#> 
+#> time zone: Europe/Berlin
+#> tzcode source: internal
+#> 
+#> attached base packages:
+#> [1] stats4    stats     graphics  grDevices utils     datasets  methods  
+#> [8] base     
+#> 
+#> other attached packages:
+#>  [1] iSEEindex_1.2.0             iSEEhub_1.6.0              
+#>  [3] ExperimentHub_2.12.0        AnnotationHub_3.12.0       
+#>  [5] BiocFileCache_2.12.0        dbplyr_2.5.0               
+#>  [7] iSEEfier_1.0.0              iSEEpathways_1.2.0         
+#>  [9] iSEEde_1.2.0                iSEE_2.15.1                
+#> [11] SingleCellExperiment_1.26.0 SummarizedExperiment_1.33.3
+#> [13] Biobase_2.64.0              GenomicRanges_1.55.4       
+#> [15] GenomeInfoDb_1.40.0         IRanges_2.38.0             
+#> [17] S4Vectors_0.42.0            BiocGenerics_0.50.0        
+#> [19] MatrixGenerics_1.16.0       matrixStats_1.3.0          
+#> [21] BiocStyle_2.32.0           
+#> 
+#> loaded via a namespace (and not attached):
+#>   [1] RColorBrewer_1.1-3      rstudioapi_0.16.0       jsonlite_1.8.8         
+#>   [4] shape_1.4.6.1           magrittr_2.0.3          farver_2.1.1           
+#>   [7] rmarkdown_2.26          GlobalOptions_0.1.2     fs_1.6.4               
+#>  [10] zlibbioc_1.50.0         ragg_1.3.0              vctrs_0.6.5            
+#>  [13] memoise_2.0.1           RCurl_1.98-1.14         htmltools_0.5.8.1      
+#>  [16] S4Arrays_1.4.0          BiocBaseUtils_1.6.0     curl_5.2.1             
+#>  [19] SparseArray_1.4.0       sass_0.4.9              bslib_0.7.0            
+#>  [22] htmlwidgets_1.6.4       desc_1.4.3              fontawesome_0.5.2      
+#>  [25] listviewer_4.0.0        cachem_1.0.8            igraph_2.0.3           
+#>  [28] mime_0.12               lifecycle_1.0.4         iterators_1.0.14       
+#>  [31] pkgconfig_2.0.3         colourpicker_1.3.0      Matrix_1.7-0           
+#>  [34] R6_2.5.1                fastmap_1.1.1           GenomeInfoDbData_1.2.12
+#>  [37] shiny_1.8.1.1           clue_0.3-65             digest_0.6.35          
+#>  [40] colorspace_2.1-0        paws.storage_0.5.0      AnnotationDbi_1.65.2   
+#>  [43] DESeq2_1.44.0           RSQLite_2.3.6           textshaping_0.3.7      
+#>  [46] filelock_1.0.3          urltools_1.7.3          fansi_1.0.6            
+#>  [49] httr_1.4.7              abind_1.4-5             mgcv_1.9-1             
+#>  [52] compiler_4.4.0          bit64_4.0.5             withr_3.0.0            
+#>  [55] doParallel_1.0.17       BiocParallel_1.38.0     DBI_1.2.2              
+#>  [58] shinyAce_0.4.2          hexbin_1.28.3           highr_0.10             
+#>  [61] rappdirs_0.3.3          DelayedArray_0.30.0     rjson_0.2.21           
+#>  [64] tools_4.4.0             vipor_0.4.7             httpuv_1.6.15          
+#>  [67] glue_1.7.0              nlme_3.1-164            promises_1.3.0         
+#>  [70] grid_4.4.0              cluster_2.1.6           generics_0.1.3         
+#>  [73] gtable_0.3.5            utf8_1.2.4              XVector_0.44.0         
+#>  [76] stringr_1.5.1           BiocVersion_3.19.1      ggrepel_0.9.5          
+#>  [79] foreach_1.5.2           pillar_1.9.0            limma_3.60.0           
+#>  [82] later_1.3.2             rintrojs_0.3.4          circlize_0.4.16        
+#>  [85] splines_4.4.0           dplyr_1.1.4             lattice_0.22-6         
+#>  [88] bit_4.0.5               paws.common_0.7.2       tidyselect_1.2.1       
+#>  [91] ComplexHeatmap_2.20.0   locfit_1.5-9.9          Biostrings_2.72.0      
+#>  [94] miniUI_0.1.1.1          knitr_1.46              edgeR_4.2.0            
+#>  [97] xfun_0.43               shinydashboard_0.7.2    statmod_1.5.0          
+#> [100] iSEEhex_1.5.0           DT_0.33                 stringi_1.8.3          
+#> [103] visNetwork_2.1.2        UCSC.utils_1.0.0        yaml_2.3.8             
+#> [106] shinyWidgets_0.8.6      evaluate_0.23           codetools_0.2-20       
+#> [109] tibble_3.2.1            BiocManager_1.30.22     cli_3.6.2              
+#> [112] xtable_1.8-4            systemfonts_1.0.6       munsell_0.5.1          
+#> [115] jquerylib_0.1.4         iSEEu_1.15.1            Rcpp_1.0.12            
+#> [118] triebeard_0.4.1         png_0.1-8               parallel_4.4.0         
+#> [121] blob_1.2.4              pkgdown_2.0.9           ggplot2_3.5.1          
+#> [124] bitops_1.0-7            viridisLite_0.4.2       scales_1.3.0           
+#> [127] purrr_1.0.2             crayon_1.5.2            GetoptLong_1.0.5       
+#> [130] rlang_1.1.3             KEGGREST_1.44.0         shinyjs_2.1.0
+
+
+

References +

+
+
+Alasoo, Kaur, Julia Rodrigues, Subhankar Mukhopadhyay, Andrew J. +Knights, Alice L. Mann, Kousik Kundu, Christine Hale, Gordon Dougan, and +Daniel J. Gaffney. 2018. “Shared genetic +effects on chromatin and gene expression indicate a role for enhancer +priming in immune response.” Nature Genetics 50 +(3): 424–31. https://doi.org/10.1038/s41588-018-0046-7. +
+
+Bartneck, Joschka, Ann-Kathrin Hartmann, Lara Stein, Danielle +Arnold-Schild, Matthias Klein, Michael Stassen, Federico Marini, et al. +2023. “Tumor-infiltrating CCR2+ inflammatory +monocytes counteract specific immunotherapy.” +Frontiers in Immunology 14 (October): 1–15. https://doi.org/10.3389/fimmu.2023.1267866. +
+
+Love, Michael I, Wolfgang Huber, and Simon Anders. 2014. “Moderated estimation of fold change and dispersion for +RNA-seq data with DESeq2.” Genome Biology 15 +(12): 550. https://doi.org/10.1186/s13059-014-0550-8. +
+
+Rigby, Rachel, Kevin Rue-Albrecht, David Sims, and Jan Rehwinkel. 2023. +“Single-cell analysis of signalling responses +to type I interferons.” bioRxiv, 1–68. +
+
+Rue-Albrecht, Kevin, Federico Marini, Charlotte Soneson, and Aaron T L +Lun. 2018. “iSEE: Interactive +SummarizedExperiment Explorer.” F1000Res. 7 +(June). +
+
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var(--fa-animation-timing, linear); } + +.fa-spin-reverse { + --fa-animation-direction: reverse; } + +.fa-pulse, +.fa-spin-pulse { + -webkit-animation-name: fa-spin; + animation-name: fa-spin; + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 1s); + animation-duration: var(--fa-animation-duration, 1s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, steps(8)); + animation-timing-function: var(--fa-animation-timing, steps(8)); } + +@media (prefers-reduced-motion: reduce) { + .fa-beat, + .fa-bounce, + .fa-fade, + .fa-beat-fade, + .fa-flip, + .fa-pulse, + .fa-shake, + .fa-spin, + .fa-spin-pulse { + -webkit-animation-delay: -1ms; + animation-delay: -1ms; + -webkit-animation-duration: 1ms; + animation-duration: 1ms; + -webkit-animation-iteration-count: 1; + animation-iteration-count: 1; + -webkit-transition-delay: 0s; + transition-delay: 0s; + -webkit-transition-duration: 0s; + transition-duration: 0s; } } + +@-webkit-keyframes fa-beat { + 0%, 90% { + -webkit-transform: scale(1); + transform: scale(1); } + 45% { + -webkit-transform: scale(var(--fa-beat-scale, 1.25)); + transform: scale(var(--fa-beat-scale, 1.25)); } } + +@keyframes fa-beat { + 0%, 90% { + -webkit-transform: scale(1); + transform: scale(1); } + 45% { + -webkit-transform: scale(var(--fa-beat-scale, 1.25)); + transform: scale(var(--fa-beat-scale, 1.25)); } } + +@-webkit-keyframes fa-bounce { + 0% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } + 10% { + -webkit-transform: scale(var(--fa-bounce-start-scale-x, 1.1), var(--fa-bounce-start-scale-y, 0.9)) translateY(0); + transform: scale(var(--fa-bounce-start-scale-x, 1.1), var(--fa-bounce-start-scale-y, 0.9)) translateY(0); } + 30% { + -webkit-transform: scale(var(--fa-bounce-jump-scale-x, 0.9), var(--fa-bounce-jump-scale-y, 1.1)) translateY(var(--fa-bounce-height, -0.5em)); + transform: scale(var(--fa-bounce-jump-scale-x, 0.9), var(--fa-bounce-jump-scale-y, 1.1)) translateY(var(--fa-bounce-height, -0.5em)); } + 50% { + -webkit-transform: scale(var(--fa-bounce-land-scale-x, 1.05), var(--fa-bounce-land-scale-y, 0.95)) translateY(0); + transform: scale(var(--fa-bounce-land-scale-x, 1.05), var(--fa-bounce-land-scale-y, 0.95)) translateY(0); } + 57% { + -webkit-transform: scale(1, 1) translateY(var(--fa-bounce-rebound, -0.125em)); + transform: scale(1, 1) translateY(var(--fa-bounce-rebound, -0.125em)); } + 64% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } + 100% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } } + +@keyframes fa-bounce { + 0% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } + 10% { + -webkit-transform: scale(var(--fa-bounce-start-scale-x, 1.1), var(--fa-bounce-start-scale-y, 0.9)) translateY(0); + transform: scale(var(--fa-bounce-start-scale-x, 1.1), var(--fa-bounce-start-scale-y, 0.9)) translateY(0); } + 30% { + -webkit-transform: scale(var(--fa-bounce-jump-scale-x, 0.9), var(--fa-bounce-jump-scale-y, 1.1)) translateY(var(--fa-bounce-height, -0.5em)); + transform: scale(var(--fa-bounce-jump-scale-x, 0.9), var(--fa-bounce-jump-scale-y, 1.1)) translateY(var(--fa-bounce-height, -0.5em)); } + 50% { + -webkit-transform: scale(var(--fa-bounce-land-scale-x, 1.05), var(--fa-bounce-land-scale-y, 0.95)) translateY(0); + transform: scale(var(--fa-bounce-land-scale-x, 1.05), var(--fa-bounce-land-scale-y, 0.95)) translateY(0); } + 57% { + -webkit-transform: scale(1, 1) translateY(var(--fa-bounce-rebound, -0.125em)); + transform: scale(1, 1) translateY(var(--fa-bounce-rebound, -0.125em)); } + 64% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } + 100% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } } + +@-webkit-keyframes fa-fade { + 50% { + opacity: var(--fa-fade-opacity, 0.4); } } + +@keyframes fa-fade { + 50% { + opacity: var(--fa-fade-opacity, 0.4); } } + +@-webkit-keyframes fa-beat-fade { + 0%, 100% { + opacity: var(--fa-beat-fade-opacity, 0.4); + -webkit-transform: scale(1); + transform: scale(1); } + 50% { + opacity: 1; + -webkit-transform: scale(var(--fa-beat-fade-scale, 1.125)); + transform: scale(var(--fa-beat-fade-scale, 1.125)); } } + +@keyframes fa-beat-fade { + 0%, 100% { + opacity: var(--fa-beat-fade-opacity, 0.4); + -webkit-transform: scale(1); + transform: scale(1); } + 50% { + opacity: 1; + -webkit-transform: scale(var(--fa-beat-fade-scale, 1.125)); + transform: scale(var(--fa-beat-fade-scale, 1.125)); } } + +@-webkit-keyframes fa-flip { + 50% { + -webkit-transform: rotate3d(var(--fa-flip-x, 0), var(--fa-flip-y, 1), var(--fa-flip-z, 0), var(--fa-flip-angle, -180deg)); + transform: rotate3d(var(--fa-flip-x, 0), var(--fa-flip-y, 1), var(--fa-flip-z, 0), var(--fa-flip-angle, -180deg)); } } + +@keyframes fa-flip { + 50% { + -webkit-transform: rotate3d(var(--fa-flip-x, 0), var(--fa-flip-y, 1), var(--fa-flip-z, 0), var(--fa-flip-angle, -180deg)); + transform: rotate3d(var(--fa-flip-x, 0), var(--fa-flip-y, 1), var(--fa-flip-z, 0), var(--fa-flip-angle, -180deg)); } } + +@-webkit-keyframes fa-shake { + 0% { + -webkit-transform: rotate(-15deg); + transform: rotate(-15deg); } + 4% { + -webkit-transform: rotate(15deg); + transform: rotate(15deg); } + 8%, 24% { + -webkit-transform: rotate(-18deg); + transform: rotate(-18deg); } + 12%, 28% { + -webkit-transform: rotate(18deg); + transform: rotate(18deg); } + 16% { + -webkit-transform: rotate(-22deg); + transform: rotate(-22deg); } + 20% { + -webkit-transform: rotate(22deg); + transform: rotate(22deg); } + 32% { + -webkit-transform: rotate(-12deg); + transform: rotate(-12deg); } + 36% { + -webkit-transform: rotate(12deg); + transform: rotate(12deg); } + 40%, 100% { + -webkit-transform: rotate(0deg); + transform: rotate(0deg); } } + +@keyframes fa-shake { + 0% { + -webkit-transform: rotate(-15deg); + transform: rotate(-15deg); } + 4% { + -webkit-transform: rotate(15deg); + transform: rotate(15deg); } + 8%, 24% { + -webkit-transform: rotate(-18deg); + transform: rotate(-18deg); } + 12%, 28% { + -webkit-transform: rotate(18deg); + transform: rotate(18deg); } + 16% { + -webkit-transform: rotate(-22deg); + transform: rotate(-22deg); } + 20% { + -webkit-transform: rotate(22deg); + transform: rotate(22deg); } + 32% { + -webkit-transform: rotate(-12deg); + transform: rotate(-12deg); } + 36% { + -webkit-transform: rotate(12deg); + transform: rotate(12deg); } + 40%, 100% { + -webkit-transform: rotate(0deg); + transform: rotate(0deg); } } + +@-webkit-keyframes fa-spin { + 0% { + -webkit-transform: rotate(0deg); + transform: rotate(0deg); } + 100% { + -webkit-transform: rotate(360deg); + transform: rotate(360deg); } } + +@keyframes fa-spin { + 0% { + -webkit-transform: rotate(0deg); + transform: rotate(0deg); } + 100% { + -webkit-transform: rotate(360deg); + transform: rotate(360deg); } } + +.fa-rotate-90 { + -webkit-transform: rotate(90deg); + transform: rotate(90deg); } + +.fa-rotate-180 { + -webkit-transform: rotate(180deg); + transform: rotate(180deg); } + +.fa-rotate-270 { + -webkit-transform: rotate(270deg); + transform: rotate(270deg); } + +.fa-flip-horizontal { + -webkit-transform: scale(-1, 1); + transform: scale(-1, 1); } + +.fa-flip-vertical { + -webkit-transform: scale(1, -1); + transform: scale(1, -1); } + +.fa-flip-both, +.fa-flip-horizontal.fa-flip-vertical { + -webkit-transform: scale(-1, -1); + transform: scale(-1, -1); } + +.fa-rotate-by { + -webkit-transform: rotate(var(--fa-rotate-angle, none)); + transform: rotate(var(--fa-rotate-angle, none)); } + +.fa-stack { + display: inline-block; + height: 2em; + line-height: 2em; + position: relative; + vertical-align: middle; + width: 2.5em; } + +.fa-stack-1x, +.fa-stack-2x { + left: 0; + position: absolute; + text-align: center; + width: 100%; + z-index: var(--fa-stack-z-index, auto); } + +.fa-stack-1x { + line-height: inherit; } + +.fa-stack-2x { + font-size: 2em; } + +.fa-inverse { + color: var(--fa-inverse, #fff); } + +/* Font Awesome uses the Unicode Private Use Area (PUA) to ensure screen +readers do not read off random characters that represent icons */ + +.fa-0::before { + content: "\30"; } + +.fa-1::before { + content: "\31"; } + +.fa-2::before { + content: "\32"; } + +.fa-3::before { + content: "\33"; } + +.fa-4::before { + content: "\34"; } + +.fa-5::before { + content: "\35"; } + +.fa-6::before { + content: "\36"; } + +.fa-7::before { + content: "\37"; } + +.fa-8::before { + content: "\38"; } + +.fa-9::before { + content: "\39"; } + +.fa-fill-drip::before { + content: "\f576"; } + +.fa-arrows-to-circle::before { + content: "\e4bd"; } + +.fa-circle-chevron-right::before { + content: "\f138"; } + +.fa-chevron-circle-right::before { + content: "\f138"; } + +.fa-at::before { + content: "\40"; } + +.fa-trash-can::before { + content: "\f2ed"; } + +.fa-trash-alt::before { + content: "\f2ed"; } + +.fa-text-height::before { + content: "\f034"; } + +.fa-user-xmark::before { + content: "\f235"; } + +.fa-user-times::before { + content: "\f235"; } + +.fa-stethoscope::before { + content: "\f0f1"; } + +.fa-message::before { + content: "\f27a"; } + +.fa-comment-alt::before { + content: "\f27a"; } + +.fa-info::before { + content: "\f129"; } + +.fa-down-left-and-up-right-to-center::before { + content: "\f422"; } + +.fa-compress-alt::before { + content: "\f422"; } + +.fa-explosion::before { + content: "\e4e9"; } + +.fa-file-lines::before { + content: "\f15c"; } + +.fa-file-alt::before { + content: "\f15c"; } + +.fa-file-text::before { + content: "\f15c"; } + +.fa-wave-square::before { + content: "\f83e"; } + +.fa-ring::before { + content: "\f70b"; } + +.fa-building-un::before { + content: "\e4d9"; } + +.fa-dice-three::before { + content: "\f527"; } + +.fa-calendar-days::before { + content: "\f073"; } + +.fa-calendar-alt::before { + content: "\f073"; } + +.fa-anchor-circle-check::before { + content: "\e4aa"; } + +.fa-building-circle-arrow-right::before { + content: "\e4d1"; } + +.fa-volleyball::before { + content: "\f45f"; } + +.fa-volleyball-ball::before { + content: "\f45f"; } + +.fa-arrows-up-to-line::before { + content: "\e4c2"; } + +.fa-sort-down::before { + content: "\f0dd"; } + +.fa-sort-desc::before { + content: "\f0dd"; } + +.fa-circle-minus::before { + content: "\f056"; } + +.fa-minus-circle::before { + content: "\f056"; } + +.fa-door-open::before { + content: "\f52b"; } + +.fa-right-from-bracket::before { + content: "\f2f5"; } + +.fa-sign-out-alt::before { + content: "\f2f5"; } + +.fa-atom::before { + content: "\f5d2"; } + +.fa-soap::before { + content: "\e06e"; } + +.fa-icons::before { + content: "\f86d"; } + +.fa-heart-music-camera-bolt::before { + content: "\f86d"; } + +.fa-microphone-lines-slash::before { + content: "\f539"; } + +.fa-microphone-alt-slash::before { + content: "\f539"; } + +.fa-bridge-circle-check::before { + content: "\e4c9"; } + +.fa-pump-medical::before { + content: "\e06a"; } + +.fa-fingerprint::before { + content: "\f577"; } + +.fa-hand-point-right::before { + content: "\f0a4"; } + +.fa-magnifying-glass-location::before { + content: "\f689"; } + +.fa-search-location::before { + content: "\f689"; } + +.fa-forward-step::before { + content: "\f051"; } + +.fa-step-forward::before { + content: "\f051"; } + +.fa-face-smile-beam::before { + content: "\f5b8"; } + +.fa-smile-beam::before { + content: "\f5b8"; } + +.fa-flag-checkered::before { + content: "\f11e"; } + +.fa-football::before { + content: "\f44e"; } + +.fa-football-ball::before { + content: "\f44e"; } + +.fa-school-circle-exclamation::before { + content: "\e56c"; } + +.fa-crop::before { + content: "\f125"; } + +.fa-angles-down::before { + content: "\f103"; } + +.fa-angle-double-down::before { + content: "\f103"; } + +.fa-users-rectangle::before { + content: "\e594"; } + +.fa-people-roof::before { + content: "\e537"; } + +.fa-people-line::before { + content: "\e534"; } + +.fa-beer-mug-empty::before { + content: "\f0fc"; } + +.fa-beer::before { + content: "\f0fc"; } + +.fa-diagram-predecessor::before { + content: "\e477"; } + +.fa-arrow-up-long::before { + content: "\f176"; } + +.fa-long-arrow-up::before { + content: "\f176"; } + +.fa-fire-flame-simple::before { + content: "\f46a"; } + +.fa-burn::before { + content: "\f46a"; } + +.fa-person::before { + content: "\f183"; } + +.fa-male::before { + content: "\f183"; } + +.fa-laptop::before { + content: "\f109"; } + +.fa-file-csv::before { + content: "\f6dd"; } + +.fa-menorah::before { + content: "\f676"; } + +.fa-truck-plane::before { + content: "\e58f"; } + +.fa-record-vinyl::before { + content: "\f8d9"; } + +.fa-face-grin-stars::before { + content: "\f587"; } + +.fa-grin-stars::before { + content: "\f587"; } + +.fa-bong::before { + content: "\f55c"; } + +.fa-spaghetti-monster-flying::before { + content: "\f67b"; } + +.fa-pastafarianism::before { + content: "\f67b"; } + +.fa-arrow-down-up-across-line::before { + content: "\e4af"; } + +.fa-spoon::before { + content: "\f2e5"; } + +.fa-utensil-spoon::before { + content: "\f2e5"; } + +.fa-jar-wheat::before { + content: "\e517"; } + +.fa-envelopes-bulk::before { + content: "\f674"; } + +.fa-mail-bulk::before { + content: "\f674"; } + +.fa-file-circle-exclamation::before { + content: "\e4eb"; } + +.fa-circle-h::before { + content: "\f47e"; } + +.fa-hospital-symbol::before { + content: "\f47e"; } + +.fa-pager::before { + content: "\f815"; } + +.fa-address-book::before { + content: "\f2b9"; } + +.fa-contact-book::before { + content: "\f2b9"; } + +.fa-strikethrough::before { + content: "\f0cc"; } + +.fa-k::before { + content: "\4b"; } + +.fa-landmark-flag::before { + content: "\e51c"; } + +.fa-pencil::before { + content: "\f303"; } + +.fa-pencil-alt::before { + content: "\f303"; } + +.fa-backward::before { + content: "\f04a"; } + +.fa-caret-right::before { + content: "\f0da"; } + +.fa-comments::before { + content: "\f086"; } + +.fa-paste::before { + content: "\f0ea"; } + +.fa-file-clipboard::before { + content: "\f0ea"; } + +.fa-code-pull-request::before { + content: "\e13c"; } + +.fa-clipboard-list::before { + content: "\f46d"; } + +.fa-truck-ramp-box::before { + content: "\f4de"; } + +.fa-truck-loading::before { + content: "\f4de"; } + +.fa-user-check::before { + content: "\f4fc"; } + +.fa-vial-virus::before { + content: "\e597"; } + +.fa-sheet-plastic::before { + content: "\e571"; } + +.fa-blog::before { + content: "\f781"; } + +.fa-user-ninja::before { + content: "\f504"; } + +.fa-person-arrow-up-from-line::before { + content: "\e539"; } + +.fa-scroll-torah::before { + content: "\f6a0"; } + +.fa-torah::before { + content: "\f6a0"; } + +.fa-broom-ball::before { + content: "\f458"; } + +.fa-quidditch::before { + content: "\f458"; } + +.fa-quidditch-broom-ball::before { + content: "\f458"; } + +.fa-toggle-off::before { + content: "\f204"; } + +.fa-box-archive::before { + content: "\f187"; } + +.fa-archive::before { + content: "\f187"; } + +.fa-person-drowning::before { + content: "\e545"; } + +.fa-arrow-down-9-1::before { + content: "\f886"; } + +.fa-sort-numeric-desc::before { + content: "\f886"; } + +.fa-sort-numeric-down-alt::before { + content: "\f886"; } + +.fa-face-grin-tongue-squint::before { + content: "\f58a"; } + +.fa-grin-tongue-squint::before { + content: "\f58a"; } + +.fa-spray-can::before { + content: "\f5bd"; } + +.fa-truck-monster::before { + content: "\f63b"; } + +.fa-w::before { + content: "\57"; } + +.fa-earth-africa::before { + content: "\f57c"; } + +.fa-globe-africa::before { + content: "\f57c"; } + +.fa-rainbow::before { + content: "\f75b"; } + +.fa-circle-notch::before { + content: "\f1ce"; } + +.fa-tablet-screen-button::before { + content: "\f3fa"; } + +.fa-tablet-alt::before { + content: "\f3fa"; } + +.fa-paw::before { + content: "\f1b0"; } + +.fa-cloud::before { + content: "\f0c2"; } + +.fa-trowel-bricks::before { + content: "\e58a"; } + +.fa-face-flushed::before { + content: "\f579"; } + +.fa-flushed::before { + content: "\f579"; } + +.fa-hospital-user::before { + content: "\f80d"; } + +.fa-tent-arrow-left-right::before { + content: "\e57f"; } + +.fa-gavel::before { + content: "\f0e3"; } + +.fa-legal::before { + content: "\f0e3"; } + +.fa-binoculars::before { + content: "\f1e5"; } + +.fa-microphone-slash::before { + content: "\f131"; } + +.fa-box-tissue::before { + content: "\e05b"; } + +.fa-motorcycle::before { + content: "\f21c"; } + +.fa-bell-concierge::before { + content: "\f562"; } + +.fa-concierge-bell::before { + content: "\f562"; } + +.fa-pen-ruler::before { + content: "\f5ae"; } + +.fa-pencil-ruler::before { + content: "\f5ae"; } + +.fa-people-arrows::before { + content: "\e068"; } + +.fa-people-arrows-left-right::before { + content: "\e068"; } + +.fa-mars-and-venus-burst::before { + content: "\e523"; } + +.fa-square-caret-right::before { + content: "\f152"; } + +.fa-caret-square-right::before { + content: "\f152"; } + +.fa-scissors::before { + content: "\f0c4"; } + +.fa-cut::before { + content: "\f0c4"; } + +.fa-sun-plant-wilt::before { + content: "\e57a"; } + +.fa-toilets-portable::before { + content: "\e584"; } + +.fa-hockey-puck::before { + content: "\f453"; } + +.fa-table::before { + content: "\f0ce"; } + +.fa-magnifying-glass-arrow-right::before { + content: "\e521"; } + +.fa-tachograph-digital::before { + content: "\f566"; } + +.fa-digital-tachograph::before { + content: "\f566"; } + +.fa-users-slash::before { + content: "\e073"; } + +.fa-clover::before { + content: "\e139"; } + +.fa-reply::before { + content: "\f3e5"; } + +.fa-mail-reply::before { + content: "\f3e5"; } + +.fa-star-and-crescent::before { + content: "\f699"; } + +.fa-house-fire::before { + content: "\e50c"; } + +.fa-square-minus::before { + content: "\f146"; } + +.fa-minus-square::before { + content: "\f146"; } + +.fa-helicopter::before { + content: "\f533"; } + +.fa-compass::before { + content: "\f14e"; } + +.fa-square-caret-down::before { + content: "\f150"; } + +.fa-caret-square-down::before { + content: "\f150"; } + +.fa-file-circle-question::before { + content: "\e4ef"; } + +.fa-laptop-code::before { + content: "\f5fc"; } + +.fa-swatchbook::before { + content: "\f5c3"; } + +.fa-prescription-bottle::before { + content: "\f485"; } + +.fa-bars::before { + content: "\f0c9"; } + +.fa-navicon::before { + content: "\f0c9"; } + +.fa-people-group::before { + content: "\e533"; } + +.fa-hourglass-end::before { + content: "\f253"; } + +.fa-hourglass-3::before { + content: "\f253"; } + +.fa-heart-crack::before { + content: "\f7a9"; } + +.fa-heart-broken::before { + content: "\f7a9"; } + +.fa-square-up-right::before { + content: "\f360"; } + +.fa-external-link-square-alt::before { + content: "\f360"; } + +.fa-face-kiss-beam::before { + content: "\f597"; } + +.fa-kiss-beam::before { + content: "\f597"; } + +.fa-film::before { + content: "\f008"; } + +.fa-ruler-horizontal::before { + content: "\f547"; } + +.fa-people-robbery::before { + content: "\e536"; } + +.fa-lightbulb::before { + content: "\f0eb"; } + +.fa-caret-left::before { + content: "\f0d9"; } + +.fa-circle-exclamation::before { + content: "\f06a"; } + +.fa-exclamation-circle::before { + content: "\f06a"; } + +.fa-school-circle-xmark::before { + content: "\e56d"; } + +.fa-arrow-right-from-bracket::before { + content: "\f08b"; } + +.fa-sign-out::before { + content: "\f08b"; } + +.fa-circle-chevron-down::before { + content: "\f13a"; } + +.fa-chevron-circle-down::before { + content: "\f13a"; } + +.fa-unlock-keyhole::before { + content: "\f13e"; } + +.fa-unlock-alt::before { + content: "\f13e"; } + +.fa-cloud-showers-heavy::before { + content: "\f740"; } + +.fa-headphones-simple::before { + content: "\f58f"; } + +.fa-headphones-alt::before { + content: "\f58f"; } + +.fa-sitemap::before { + content: "\f0e8"; } + +.fa-circle-dollar-to-slot::before { + content: "\f4b9"; } + +.fa-donate::before { + content: "\f4b9"; } + +.fa-memory::before { + content: "\f538"; } + +.fa-road-spikes::before { + content: "\e568"; } + +.fa-fire-burner::before { + content: "\e4f1"; } + +.fa-flag::before { + content: "\f024"; } + +.fa-hanukiah::before { + content: "\f6e6"; } + +.fa-feather::before { + content: "\f52d"; } + +.fa-volume-low::before { + content: "\f027"; } + +.fa-volume-down::before { + content: "\f027"; } + +.fa-comment-slash::before { + content: "\f4b3"; } + +.fa-cloud-sun-rain::before { + content: "\f743"; } + +.fa-compress::before { + content: "\f066"; } + +.fa-wheat-awn::before { + content: "\e2cd"; } + +.fa-wheat-alt::before { + 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content: "\f487"; } + +.fa-procedures::before { + content: "\f487"; } + +.fa-shuttle-space::before { + content: "\f197"; } + +.fa-space-shuttle::before { + content: "\f197"; } + +.fa-face-laugh::before { + content: "\f599"; } + +.fa-laugh::before { + content: "\f599"; } + +.fa-folder-open::before { + content: "\f07c"; } + +.fa-heart-circle-plus::before { + content: "\e500"; } + +.fa-code-fork::before { + content: "\e13b"; } + +.fa-city::before { + content: "\f64f"; } + +.fa-microphone-lines::before { + content: "\f3c9"; } + +.fa-microphone-alt::before { + content: "\f3c9"; } + +.fa-pepper-hot::before { + content: "\f816"; } + +.fa-unlock::before { + content: "\f09c"; } + +.fa-colon-sign::before { + content: "\e140"; } + +.fa-headset::before { + content: "\f590"; } + +.fa-store-slash::before { + content: "\e071"; } + +.fa-road-circle-xmark::before { + content: "\e566"; } + +.fa-user-minus::before { + content: "\f503"; } + +.fa-mars-stroke-up::before { + content: "\f22a"; } + 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} + +.fa-manat-sign::before { + content: "\e1d5"; } + +.fa-not-equal::before { + content: "\f53e"; } + +.fa-border-top-left::before { + content: "\f853"; } + +.fa-border-style::before { + content: "\f853"; } + +.fa-map-location-dot::before { + content: "\f5a0"; } + +.fa-map-marked-alt::before { + content: "\f5a0"; } + +.fa-jedi::before { + content: "\f669"; } + +.fa-square-poll-vertical::before { + content: "\f681"; } + +.fa-poll::before { + content: "\f681"; } + +.fa-mug-hot::before { + content: "\f7b6"; } + +.fa-car-battery::before { + content: "\f5df"; } + +.fa-battery-car::before { + content: "\f5df"; } + +.fa-gift::before { + content: "\f06b"; } + +.fa-dice-two::before { + content: "\f528"; } + +.fa-chess-queen::before { + content: "\f445"; } + +.fa-glasses::before { + content: "\f530"; } + +.fa-chess-board::before { + content: "\f43c"; } + +.fa-building-circle-check::before { + content: "\e4d2"; } + +.fa-person-chalkboard::before { + content: "\e53d"; } + 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content: "\f7c5"; } + +.fa-skating::before { + content: "\f7c5"; } + +.fa-filter-circle-dollar::before { + content: "\f662"; } + +.fa-funnel-dollar::before { + content: "\f662"; } + +.fa-camera-retro::before { + content: "\f083"; } + +.fa-circle-arrow-down::before { + content: "\f0ab"; } + +.fa-arrow-circle-down::before { + content: "\f0ab"; } + +.fa-file-import::before { + content: "\f56f"; } + +.fa-arrow-right-to-file::before { + content: "\f56f"; } + +.fa-square-arrow-up-right::before { + content: "\f14c"; } + +.fa-external-link-square::before { + content: "\f14c"; } + +.fa-box-open::before { + content: "\f49e"; } + +.fa-scroll::before { + content: "\f70e"; } + +.fa-spa::before { + content: "\f5bb"; } + +.fa-location-pin-lock::before { + content: "\e51f"; } + +.fa-pause::before { + content: "\f04c"; } + +.fa-hill-avalanche::before { + content: "\e507"; } + +.fa-temperature-empty::before { + content: "\f2cb"; } + +.fa-temperature-0::before { + content: "\f2cb"; } + 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} + +.fa-hand-middle-finger::before { + content: "\f806"; } + +.fa-percent::before { + content: "\25"; } + +.fa-percentage::before { + content: "\25"; } + +.fa-truck-moving::before { + content: "\f4df"; } + +.fa-glass-water-droplet::before { + content: "\e4f5"; } + +.fa-display::before { + content: "\e163"; } + +.fa-face-smile::before { + content: "\f118"; } + +.fa-smile::before { + content: "\f118"; } + +.fa-thumbtack::before { + content: "\f08d"; } + +.fa-thumb-tack::before { + content: "\f08d"; } + +.fa-trophy::before { + content: "\f091"; } + +.fa-person-praying::before { + content: "\f683"; } + +.fa-pray::before { + content: "\f683"; } + +.fa-hammer::before { + content: "\f6e3"; } + +.fa-hand-peace::before { + content: "\f25b"; } + +.fa-rotate::before { + content: "\f2f1"; } + +.fa-sync-alt::before { + content: "\f2f1"; } + +.fa-spinner::before { + content: "\f110"; } + +.fa-robot::before { + content: "\f544"; } + +.fa-peace::before { + content: "\f67c"; } + +.fa-gears::before { + content: "\f085"; } + +.fa-cogs::before { + content: "\f085"; } + +.fa-warehouse::before { + content: "\f494"; } + +.fa-arrow-up-right-dots::before { + content: "\e4b7"; } + +.fa-splotch::before { + content: "\f5bc"; } + +.fa-face-grin-hearts::before { + content: "\f584"; } + +.fa-grin-hearts::before { + content: "\f584"; } + +.fa-dice-four::before { + content: "\f524"; } + +.fa-sim-card::before { + content: "\f7c4"; } + +.fa-transgender::before { + content: "\f225"; } + +.fa-transgender-alt::before { + content: "\f225"; } + +.fa-mercury::before { + content: "\f223"; } + +.fa-arrow-turn-down::before { + content: "\f149"; } + +.fa-level-down::before { + content: "\f149"; } + +.fa-person-falling-burst::before { + content: "\e547"; } + +.fa-award::before { + content: "\f559"; } + +.fa-ticket-simple::before { + content: "\f3ff"; } + +.fa-ticket-alt::before { + content: "\f3ff"; } + +.fa-building::before { + content: "\f1ad"; } + +.fa-angles-left::before { + content: "\f100"; } + 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+.fa-pen-nib::before { + content: "\f5ad"; } + +.fa-tent-arrow-turn-left::before { + content: "\e580"; } + +.fa-tents::before { + content: "\e582"; } + +.fa-wand-magic::before { + content: "\f0d0"; } + +.fa-magic::before { + content: "\f0d0"; } + +.fa-dog::before { + content: "\f6d3"; } + +.fa-carrot::before { + content: "\f787"; } + +.fa-moon::before { + content: "\f186"; } + +.fa-wine-glass-empty::before { + content: "\f5ce"; } + +.fa-wine-glass-alt::before { + content: "\f5ce"; } + +.fa-cheese::before { + content: "\f7ef"; } + +.fa-yin-yang::before { + content: "\f6ad"; } + +.fa-music::before { + content: "\f001"; } + +.fa-code-commit::before { + content: "\f386"; } + +.fa-temperature-low::before { + content: "\f76b"; } + +.fa-person-biking::before { + content: "\f84a"; } + +.fa-biking::before { + content: "\f84a"; } + +.fa-broom::before { + content: "\f51a"; } + +.fa-shield-heart::before { + content: "\e574"; } + +.fa-gopuram::before { + content: "\f664"; } + +.fa-earth-oceania::before { + content: "\e47b"; } + +.fa-globe-oceania::before { + content: "\e47b"; } + +.fa-square-xmark::before { + content: "\f2d3"; } + +.fa-times-square::before { + content: "\f2d3"; } + +.fa-xmark-square::before { + content: "\f2d3"; } + +.fa-hashtag::before { + content: "\23"; } + +.fa-up-right-and-down-left-from-center::before { + content: "\f424"; } + +.fa-expand-alt::before { + content: "\f424"; } + +.fa-oil-can::before { + content: "\f613"; } + +.fa-t::before { + content: "\54"; } + +.fa-hippo::before { + content: "\f6ed"; } + +.fa-chart-column::before { + content: "\e0e3"; } + +.fa-infinity::before { + content: "\f534"; } + +.fa-vial-circle-check::before { + content: "\e596"; } + +.fa-person-arrow-down-to-line::before { + content: "\e538"; } + +.fa-voicemail::before { + content: "\f897"; } + +.fa-fan::before { + content: "\f863"; } + +.fa-person-walking-luggage::before { + content: "\e554"; } + +.fa-up-down::before { + content: "\f338"; } + +.fa-arrows-alt-v::before { + content: "\f338"; } + +.fa-cloud-moon-rain::before { + content: "\f73c"; } + +.fa-calendar::before { + content: "\f133"; } + +.fa-trailer::before { + content: "\e041"; } + +.fa-bahai::before { + content: "\f666"; } + +.fa-haykal::before { + content: "\f666"; } + +.fa-sd-card::before { + content: "\f7c2"; } + +.fa-dragon::before { + content: "\f6d5"; } + +.fa-shoe-prints::before { + content: "\f54b"; } + +.fa-circle-plus::before { + content: "\f055"; } + +.fa-plus-circle::before { + content: "\f055"; } + +.fa-face-grin-tongue-wink::before { + content: "\f58b"; } + +.fa-grin-tongue-wink::before { + content: "\f58b"; } + +.fa-hand-holding::before { + content: "\f4bd"; } + +.fa-plug-circle-exclamation::before { + content: "\e55d"; } + +.fa-link-slash::before { + content: "\f127"; } + +.fa-chain-broken::before { + content: "\f127"; } + +.fa-chain-slash::before { + content: "\f127"; } + +.fa-unlink::before { + content: "\f127"; } + +.fa-clone::before { + content: "\f24d"; } + +.fa-person-walking-arrow-loop-left::before { + content: "\e551"; } + +.fa-arrow-up-z-a::before { + content: "\f882"; } + +.fa-sort-alpha-up-alt::before { + content: "\f882"; } + +.fa-fire-flame-curved::before { + content: "\f7e4"; } + +.fa-fire-alt::before { + content: "\f7e4"; } + +.fa-tornado::before { + content: "\f76f"; } + +.fa-file-circle-plus::before { + content: "\e494"; } + +.fa-book-quran::before { + content: "\f687"; } + +.fa-quran::before { + content: "\f687"; } + +.fa-anchor::before { + content: "\f13d"; } + +.fa-border-all::before { + content: "\f84c"; } + +.fa-face-angry::before { + content: "\f556"; } + +.fa-angry::before { + content: "\f556"; } + +.fa-cookie-bite::before { + content: "\f564"; } + +.fa-arrow-trend-down::before { + content: "\e097"; } + +.fa-rss::before { + content: "\f09e"; } + +.fa-feed::before { + content: "\f09e"; } + +.fa-draw-polygon::before { + content: "\f5ee"; } + +.fa-scale-balanced::before { + content: "\f24e"; } + +.fa-balance-scale::before { + content: "\f24e"; } + +.fa-gauge-simple-high::before { + content: "\f62a"; } + +.fa-tachometer::before { + content: "\f62a"; } + +.fa-tachometer-fast::before { + content: "\f62a"; } + +.fa-shower::before { + content: "\f2cc"; } + +.fa-desktop::before { + content: "\f390"; } + +.fa-desktop-alt::before { + content: "\f390"; } + +.fa-m::before { + content: "\4d"; } + +.fa-table-list::before { + content: "\f00b"; } + +.fa-th-list::before { + content: "\f00b"; } + +.fa-comment-sms::before { + content: "\f7cd"; } + +.fa-sms::before { + content: "\f7cd"; } + +.fa-book::before { + content: "\f02d"; } + +.fa-user-plus::before { + content: "\f234"; } + +.fa-check::before { + content: "\f00c"; } + +.fa-battery-three-quarters::before { + content: "\f241"; } + +.fa-battery-4::before { + content: "\f241"; } + +.fa-house-circle-check::before { + content: "\e509"; } + +.fa-angle-left::before { + content: "\f104"; } + +.fa-diagram-successor::before { + content: "\e47a"; } + +.fa-truck-arrow-right::before { + content: "\e58b"; } + +.fa-arrows-split-up-and-left::before { + content: "\e4bc"; } + +.fa-hand-fist::before { + content: "\f6de"; } + +.fa-fist-raised::before { + content: "\f6de"; } + +.fa-cloud-moon::before { + content: "\f6c3"; } + +.fa-briefcase::before { + content: "\f0b1"; } + +.fa-person-falling::before { + content: "\e546"; } + +.fa-image-portrait::before { + content: "\f3e0"; } + +.fa-portrait::before { + content: "\f3e0"; } + +.fa-user-tag::before { + content: "\f507"; } + +.fa-rug::before { + content: "\e569"; } + +.fa-earth-europe::before { + content: "\f7a2"; } + +.fa-globe-europe::before { + content: "\f7a2"; } + +.fa-cart-flatbed-suitcase::before { + content: "\f59d"; } + +.fa-luggage-cart::before { + content: "\f59d"; } + +.fa-rectangle-xmark::before { + content: "\f410"; } + +.fa-rectangle-times::before { + content: "\f410"; } + +.fa-times-rectangle::before { + content: "\f410"; } + +.fa-window-close::before { + content: "\f410"; } + +.fa-baht-sign::before { + content: "\e0ac"; } + +.fa-book-open::before { + content: "\f518"; } + +.fa-book-journal-whills::before { + content: "\f66a"; } + +.fa-journal-whills::before { + content: "\f66a"; } + +.fa-handcuffs::before { + content: "\e4f8"; } + +.fa-triangle-exclamation::before { + content: "\f071"; } + +.fa-exclamation-triangle::before { + content: "\f071"; } + +.fa-warning::before { + content: "\f071"; } + +.fa-database::before { + content: "\f1c0"; } + +.fa-share::before { + content: "\f064"; } + +.fa-arrow-turn-right::before { + content: "\f064"; } + +.fa-mail-forward::before { + content: "\f064"; } + +.fa-bottle-droplet::before { + content: "\e4c4"; } + +.fa-mask-face::before { + content: "\e1d7"; } + +.fa-hill-rockslide::before { + content: "\e508"; } + +.fa-right-left::before { + content: "\f362"; } + +.fa-exchange-alt::before { + content: "\f362"; } + +.fa-paper-plane::before { + content: "\f1d8"; } + +.fa-road-circle-exclamation::before { + content: "\e565"; } + +.fa-dungeon::before { + content: "\f6d9"; } + +.fa-align-right::before { + content: "\f038"; } + +.fa-money-bill-1-wave::before { + content: "\f53b"; } + +.fa-money-bill-wave-alt::before { + content: "\f53b"; } + +.fa-life-ring::before { + content: "\f1cd"; } + +.fa-hands::before { + content: "\f2a7"; } + +.fa-sign-language::before { + content: "\f2a7"; } + +.fa-signing::before { + content: "\f2a7"; } + +.fa-calendar-day::before { + content: "\f783"; } + +.fa-water-ladder::before { + content: "\f5c5"; } + +.fa-ladder-water::before { + content: "\f5c5"; } + +.fa-swimming-pool::before { + content: "\f5c5"; } + +.fa-arrows-up-down::before { + content: "\f07d"; } + +.fa-arrows-v::before { + content: "\f07d"; } + +.fa-face-grimace::before { + content: "\f57f"; } + +.fa-grimace::before { + content: "\f57f"; } + +.fa-wheelchair-move::before { + content: "\e2ce"; } + +.fa-wheelchair-alt::before { + content: "\e2ce"; } + +.fa-turn-down::before { + content: "\f3be"; } + +.fa-level-down-alt::before { + content: "\f3be"; } + +.fa-person-walking-arrow-right::before { + content: "\e552"; } + +.fa-square-envelope::before { + content: "\f199"; } + +.fa-envelope-square::before { + content: "\f199"; } + +.fa-dice::before { + content: "\f522"; } + +.fa-bowling-ball::before { + content: "\f436"; } + +.fa-brain::before { + content: "\f5dc"; } + +.fa-bandage::before { + content: "\f462"; } + +.fa-band-aid::before { + content: "\f462"; } + +.fa-calendar-minus::before { + content: "\f272"; } + +.fa-circle-xmark::before { + content: "\f057"; } + +.fa-times-circle::before { + content: "\f057"; } + +.fa-xmark-circle::before { + content: "\f057"; } + +.fa-gifts::before { + content: "\f79c"; } + +.fa-hotel::before { + content: "\f594"; } + +.fa-earth-asia::before { + content: "\f57e"; } + +.fa-globe-asia::before { + content: "\f57e"; } + +.fa-id-card-clip::before { + content: "\f47f"; } + +.fa-id-card-alt::before { + content: "\f47f"; } + +.fa-magnifying-glass-plus::before { + content: "\f00e"; } + +.fa-search-plus::before { + content: "\f00e"; } + +.fa-thumbs-up::before { + content: "\f164"; } + +.fa-user-clock::before { + content: "\f4fd"; } + +.fa-hand-dots::before { + content: "\f461"; } + +.fa-allergies::before { + content: "\f461"; } + +.fa-file-invoice::before { + content: "\f570"; } + +.fa-window-minimize::before { + content: "\f2d1"; } + +.fa-mug-saucer::before { + content: "\f0f4"; } + +.fa-coffee::before { + content: "\f0f4"; } + +.fa-brush::before { + content: "\f55d"; } + +.fa-mask::before { + content: "\f6fa"; } + +.fa-magnifying-glass-minus::before { + content: "\f010"; } + +.fa-search-minus::before { + content: "\f010"; } + +.fa-ruler-vertical::before { + content: "\f548"; } + +.fa-user-large::before { + content: "\f406"; } + +.fa-user-alt::before { + content: "\f406"; } + +.fa-train-tram::before { + content: "\e5b4"; } + +.fa-user-nurse::before { + content: "\f82f"; } + +.fa-syringe::before { + content: "\f48e"; } + +.fa-cloud-sun::before { + content: "\f6c4"; } + +.fa-stopwatch-20::before { + content: "\e06f"; } + +.fa-square-full::before { + content: "\f45c"; } + +.fa-magnet::before { + content: "\f076"; } + +.fa-jar::before { + content: "\e516"; } + +.fa-note-sticky::before { + content: "\f249"; } + +.fa-sticky-note::before { + content: "\f249"; } + +.fa-bug-slash::before { + content: "\e490"; } + +.fa-arrow-up-from-water-pump::before { + content: "\e4b6"; } + +.fa-bone::before { + content: "\f5d7"; } + +.fa-user-injured::before { + content: "\f728"; } + +.fa-face-sad-tear::before { + content: "\f5b4"; } + +.fa-sad-tear::before { + content: "\f5b4"; } + +.fa-plane::before { + content: "\f072"; } + +.fa-tent-arrows-down::before { + content: "\e581"; } + +.fa-exclamation::before { + content: "\21"; } + +.fa-arrows-spin::before { + content: "\e4bb"; } + +.fa-print::before { + content: "\f02f"; } + +.fa-turkish-lira-sign::before { + content: "\e2bb"; } + +.fa-try::before { + content: "\e2bb"; } + +.fa-turkish-lira::before { + content: "\e2bb"; } + +.fa-dollar-sign::before { + content: "\24"; } + +.fa-dollar::before { + content: "\24"; } + +.fa-usd::before { + content: "\24"; } + +.fa-x::before { + content: "\58"; } + +.fa-magnifying-glass-dollar::before { + content: "\f688"; } + +.fa-search-dollar::before { + content: "\f688"; } + +.fa-users-gear::before { + content: "\f509"; } + +.fa-users-cog::before { + content: "\f509"; } + +.fa-person-military-pointing::before { + content: "\e54a"; } + +.fa-building-columns::before { + content: "\f19c"; } + +.fa-bank::before { + content: "\f19c"; } + +.fa-institution::before { + content: "\f19c"; } + +.fa-museum::before { + content: "\f19c"; } + +.fa-university::before { + content: "\f19c"; } + +.fa-umbrella::before { + content: "\f0e9"; } + +.fa-trowel::before { + content: "\e589"; } + +.fa-d::before { + content: "\44"; } + +.fa-stapler::before { + content: "\e5af"; } + +.fa-masks-theater::before { + content: "\f630"; } + +.fa-theater-masks::before { + content: "\f630"; } + +.fa-kip-sign::before { + content: "\e1c4"; } + +.fa-hand-point-left::before { + content: "\f0a5"; } + +.fa-handshake-simple::before { + content: "\f4c6"; } + +.fa-handshake-alt::before { + content: "\f4c6"; } + +.fa-jet-fighter::before { + content: "\f0fb"; } + +.fa-fighter-jet::before { + content: "\f0fb"; } + +.fa-square-share-nodes::before { + content: "\f1e1"; } + +.fa-share-alt-square::before { + content: "\f1e1"; } + +.fa-barcode::before { + content: "\f02a"; } + +.fa-plus-minus::before { + content: "\e43c"; } + +.fa-video::before { + content: "\f03d"; } + +.fa-video-camera::before { + content: "\f03d"; } + +.fa-graduation-cap::before { + content: "\f19d"; } + +.fa-mortar-board::before { + content: "\f19d"; } + +.fa-hand-holding-medical::before { + content: "\e05c"; } + +.fa-person-circle-check::before { + content: "\e53e"; } + +.fa-turn-up::before { + content: "\f3bf"; } + +.fa-level-up-alt::before { + content: "\f3bf"; } + +.sr-only, +.fa-sr-only { + position: absolute; + width: 1px; + height: 1px; + padding: 0; + margin: -1px; + overflow: hidden; + clip: rect(0, 0, 0, 0); + white-space: nowrap; + border-width: 0; } + +.sr-only-focusable:not(:focus), +.fa-sr-only-focusable:not(:focus) { + position: absolute; + width: 1px; + height: 1px; + padding: 0; + margin: -1px; + overflow: hidden; + clip: rect(0, 0, 0, 0); + white-space: nowrap; + border-width: 0; } +:root, :host { + --fa-style-family-brands: 'Font Awesome 6 Brands'; + --fa-font-brands: normal 400 1em/1 'Font Awesome 6 Brands'; } + +@font-face { + font-family: 'Font Awesome 6 Brands'; + font-style: normal; + font-weight: 400; + font-display: block; + src: url("../webfonts/fa-brands-400.woff2") format("woff2"), url("../webfonts/fa-brands-400.ttf") format("truetype"); } + +.fab, +.fa-brands { + font-weight: 400; } + +.fa-monero:before { + content: "\f3d0"; } + +.fa-hooli:before { + content: "\f427"; } + +.fa-yelp:before { + content: "\f1e9"; } + +.fa-cc-visa:before { + content: "\f1f0"; } + +.fa-lastfm:before { + content: "\f202"; } + +.fa-shopware:before { + content: "\f5b5"; } + +.fa-creative-commons-nc:before { + content: "\f4e8"; } + +.fa-aws:before { + content: "\f375"; } + +.fa-redhat:before { + content: "\f7bc"; } + +.fa-yoast:before { + content: "\f2b1"; } + +.fa-cloudflare:before { + content: "\e07d"; } + +.fa-ups:before { + content: "\f7e0"; } + +.fa-wpexplorer:before { + content: "\f2de"; } + +.fa-dyalog:before { + content: "\f399"; } + +.fa-bity:before { + content: "\f37a"; } + +.fa-stackpath:before { + content: "\f842"; } + +.fa-buysellads:before { + content: "\f20d"; } + +.fa-first-order:before { + content: "\f2b0"; } + +.fa-modx:before { + content: "\f285"; } + +.fa-guilded:before { + content: "\e07e"; } + +.fa-vnv:before { + content: "\f40b"; } + +.fa-square-js:before { + content: "\f3b9"; } + +.fa-js-square:before { + content: "\f3b9"; } + +.fa-microsoft:before { + content: "\f3ca"; } + +.fa-qq:before { + content: "\f1d6"; } + +.fa-orcid:before { + content: "\f8d2"; } + +.fa-java:before { + content: "\f4e4"; } + +.fa-invision:before { + content: "\f7b0"; } + +.fa-creative-commons-pd-alt:before { + content: "\f4ed"; } + +.fa-centercode:before { + content: "\f380"; } + +.fa-glide-g:before { + content: "\f2a6"; } + +.fa-drupal:before { + content: "\f1a9"; } + +.fa-hire-a-helper:before { + content: "\f3b0"; } + +.fa-creative-commons-by:before { + content: "\f4e7"; } + +.fa-unity:before { + content: "\e049"; } + +.fa-whmcs:before { + content: "\f40d"; } + +.fa-rocketchat:before { + content: "\f3e8"; } + +.fa-vk:before { + content: "\f189"; } + +.fa-untappd:before { + content: "\f405"; } + +.fa-mailchimp:before { + content: "\f59e"; } + +.fa-css3-alt:before { + content: "\f38b"; } + +.fa-square-reddit:before { + content: "\f1a2"; } + +.fa-reddit-square:before { + content: "\f1a2"; } + +.fa-vimeo-v:before { + content: "\f27d"; } + +.fa-contao:before { + content: "\f26d"; } + +.fa-square-font-awesome:before { + content: "\e5ad"; } + +.fa-deskpro:before { + content: "\f38f"; } + +.fa-sistrix:before { + content: "\f3ee"; } + +.fa-square-instagram:before { + content: "\e055"; } + +.fa-instagram-square:before { + content: "\e055"; } + +.fa-battle-net:before { + content: "\f835"; } + +.fa-the-red-yeti:before { + content: "\f69d"; } + +.fa-square-hacker-news:before { + content: "\f3af"; } + +.fa-hacker-news-square:before { + content: "\f3af"; } + +.fa-edge:before { + content: "\f282"; } + +.fa-threads:before { + content: "\e618"; } + +.fa-napster:before { + content: "\f3d2"; } + +.fa-square-snapchat:before { + content: "\f2ad"; } + +.fa-snapchat-square:before { + content: "\f2ad"; } + +.fa-google-plus-g:before { + content: "\f0d5"; } + +.fa-artstation:before { + content: "\f77a"; } + +.fa-markdown:before { + content: "\f60f"; } + +.fa-sourcetree:before { + content: "\f7d3"; } + +.fa-google-plus:before { + content: "\f2b3"; } + +.fa-diaspora:before { + content: "\f791"; } + +.fa-foursquare:before { + content: "\f180"; } + +.fa-stack-overflow:before { + content: "\f16c"; } + +.fa-github-alt:before { + content: "\f113"; } + +.fa-phoenix-squadron:before { + content: "\f511"; } + +.fa-pagelines:before { + content: "\f18c"; } + +.fa-algolia:before { + content: "\f36c"; } + +.fa-red-river:before { + content: "\f3e3"; } + +.fa-creative-commons-sa:before { + content: "\f4ef"; } + +.fa-safari:before { + content: "\f267"; } + +.fa-google:before { + content: "\f1a0"; } + +.fa-square-font-awesome-stroke:before { + content: "\f35c"; } + +.fa-font-awesome-alt:before { + content: "\f35c"; } + +.fa-atlassian:before { + content: "\f77b"; } + +.fa-linkedin-in:before { + content: "\f0e1"; } + +.fa-digital-ocean:before { + content: "\f391"; } + +.fa-nimblr:before { + content: "\f5a8"; } + +.fa-chromecast:before { + content: "\f838"; } + +.fa-evernote:before { + content: "\f839"; } + +.fa-hacker-news:before { + content: "\f1d4"; } + +.fa-creative-commons-sampling:before { + content: "\f4f0"; } + +.fa-adversal:before { + content: "\f36a"; } + +.fa-creative-commons:before { + content: "\f25e"; } + +.fa-watchman-monitoring:before { + content: "\e087"; } + +.fa-fonticons:before { + content: "\f280"; } + +.fa-weixin:before { + content: "\f1d7"; } + +.fa-shirtsinbulk:before { + content: "\f214"; } + +.fa-codepen:before { + content: "\f1cb"; } + +.fa-git-alt:before { + content: "\f841"; } + +.fa-lyft:before { + content: "\f3c3"; } + +.fa-rev:before { + content: "\f5b2"; } + +.fa-windows:before { + content: "\f17a"; } + +.fa-wizards-of-the-coast:before { + content: "\f730"; } + +.fa-square-viadeo:before { + content: "\f2aa"; } + +.fa-viadeo-square:before { + content: "\f2aa"; } + +.fa-meetup:before { + content: "\f2e0"; } + +.fa-centos:before { + content: "\f789"; } + +.fa-adn:before { + content: "\f170"; } + +.fa-cloudsmith:before { + content: "\f384"; } + +.fa-pied-piper-alt:before { + content: "\f1a8"; } + 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content: "\f798"; } + +.fa-stripe-s:before { + content: "\f42a"; } + +.fa-meta:before { + content: "\e49b"; } + +.fa-laravel:before { + content: "\f3bd"; } + +.fa-hotjar:before { + content: "\f3b1"; } + +.fa-bluetooth-b:before { + content: "\f294"; } + +.fa-sticker-mule:before { + content: "\f3f7"; } + +.fa-creative-commons-zero:before { + content: "\f4f3"; } + +.fa-hips:before { + content: "\f452"; } + +.fa-behance:before { + content: "\f1b4"; } + +.fa-reddit:before { + content: "\f1a1"; } + +.fa-discord:before { + content: "\f392"; } + +.fa-chrome:before { + content: "\f268"; } + +.fa-app-store-ios:before { + content: "\f370"; } + +.fa-cc-discover:before { + content: "\f1f2"; } + +.fa-wpbeginner:before { + content: "\f297"; } + +.fa-confluence:before { + content: "\f78d"; } + +.fa-mdb:before { + content: "\f8ca"; } + +.fa-dochub:before { + content: "\f394"; } + +.fa-accessible-icon:before { + content: "\f368"; } + +.fa-ebay:before { + content: "\f4f4"; } + +.fa-amazon:before { + content: "\f270"; } + +.fa-unsplash:before { + content: "\e07c"; } + +.fa-yarn:before { + content: "\f7e3"; } + +.fa-square-steam:before { + content: "\f1b7"; } + +.fa-steam-square:before { + content: "\f1b7"; } + +.fa-500px:before { + content: "\f26e"; } + +.fa-square-vimeo:before { + content: "\f194"; } + +.fa-vimeo-square:before { + content: "\f194"; } + +.fa-asymmetrik:before { + content: "\f372"; } + +.fa-font-awesome:before { + content: "\f2b4"; } + +.fa-font-awesome-flag:before { + content: "\f2b4"; } + +.fa-font-awesome-logo-full:before { + content: "\f2b4"; } + +.fa-gratipay:before { + content: "\f184"; } + +.fa-apple:before { + content: "\f179"; } + +.fa-hive:before { + content: "\e07f"; } + +.fa-gitkraken:before { + content: "\f3a6"; } + +.fa-keybase:before { + content: "\f4f5"; } + +.fa-apple-pay:before { + content: "\f415"; } + +.fa-padlet:before { + content: "\e4a0"; } + +.fa-amazon-pay:before { + content: "\f42c"; } + +.fa-square-github:before { + content: "\f092"; } + 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+ font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-down:before { + content: "\f150"; } + +.fa.fa-toggle-down { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-toggle-down:before { + content: "\f150"; } + +.fa.fa-caret-square-o-up { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-up:before { + content: "\f151"; } + +.fa.fa-toggle-up { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-toggle-up:before { + content: "\f151"; } + +.fa.fa-caret-square-o-right { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-right:before { + content: "\f152"; } + +.fa.fa-toggle-right { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-toggle-right:before { + content: "\f152"; } + +.fa.fa-eur:before { + content: "\f153"; } + +.fa.fa-euro:before { + content: "\f153"; } + +.fa.fa-gbp:before { + content: "\f154"; } + +.fa.fa-usd:before { + content: "\24"; } + +.fa.fa-dollar:before { + content: "\24"; } + +.fa.fa-inr:before { + content: "\e1bc"; } + +.fa.fa-rupee:before { + content: "\e1bc"; } + +.fa.fa-jpy:before { + content: "\f157"; } + +.fa.fa-cny:before { + content: "\f157"; } + +.fa.fa-rmb:before { + content: "\f157"; } + +.fa.fa-yen:before { + content: "\f157"; } + +.fa.fa-rub:before { + content: "\f158"; } + +.fa.fa-ruble:before { + content: "\f158"; } + +.fa.fa-rouble:before { + content: "\f158"; } + +.fa.fa-krw:before { + content: "\f159"; } + +.fa.fa-won:before { + content: "\f159"; } + +.fa.fa-btc { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitcoin { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitcoin:before { + content: "\f15a"; } + +.fa.fa-file-text:before { + content: "\f15c"; } + +.fa.fa-sort-alpha-asc:before { + content: "\f15d"; } + +.fa.fa-sort-alpha-desc:before { + content: "\f881"; } + +.fa.fa-sort-amount-asc:before { + content: "\f884"; } + +.fa.fa-sort-amount-desc:before { + content: "\f160"; } + +.fa.fa-sort-numeric-asc:before { + content: "\f162"; } + +.fa.fa-sort-numeric-desc:before { + content: "\f886"; } + +.fa.fa-youtube-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-youtube-square:before { + content: "\f431"; } + +.fa.fa-youtube { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-xing { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-xing-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-xing-square:before { + content: "\f169"; } + +.fa.fa-youtube-play { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-youtube-play:before { + content: "\f167"; } + +.fa.fa-dropbox { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-stack-overflow { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-instagram { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-flickr { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-adn { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitbucket { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitbucket-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitbucket-square:before { + content: "\f171"; } + +.fa.fa-tumblr { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-tumblr-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-tumblr-square:before { + content: "\f174"; } + +.fa.fa-long-arrow-down:before { + content: "\f309"; } + +.fa.fa-long-arrow-up:before { + content: "\f30c"; } + +.fa.fa-long-arrow-left:before { + content: "\f30a"; } + +.fa.fa-long-arrow-right:before { + content: "\f30b"; } + +.fa.fa-apple { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-windows { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-android { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-linux { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-dribbble { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-skype { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-foursquare { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-trello { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gratipay { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gittip { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gittip:before { + content: "\f184"; } + +.fa.fa-sun-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-sun-o:before { + content: "\f185"; } + +.fa.fa-moon-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-moon-o:before { + content: "\f186"; } + +.fa.fa-vk { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-weibo { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-renren { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pagelines { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-stack-exchange { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-arrow-circle-o-right { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-arrow-circle-o-right:before { + content: "\f35a"; } + +.fa.fa-arrow-circle-o-left { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-arrow-circle-o-left:before { + content: "\f359"; } + +.fa.fa-caret-square-o-left { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-left:before { + content: "\f191"; } + +.fa.fa-toggle-left { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-toggle-left:before { + content: "\f191"; } + +.fa.fa-dot-circle-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-dot-circle-o:before { + content: "\f192"; } + +.fa.fa-vimeo-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-vimeo-square:before { + content: "\f194"; } + +.fa.fa-try:before { + content: "\e2bb"; } + +.fa.fa-turkish-lira:before { + content: "\e2bb"; } + +.fa.fa-plus-square-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-plus-square-o:before { + content: "\f0fe"; } + +.fa.fa-slack { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wordpress { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-openid { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-institution:before { + content: "\f19c"; } + +.fa.fa-bank:before { + content: "\f19c"; } + +.fa.fa-mortar-board:before { + content: "\f19d"; } + +.fa.fa-yahoo { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-google { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-reddit { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-reddit-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-reddit-square:before { + content: "\f1a2"; } + +.fa.fa-stumbleupon-circle { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-stumbleupon { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-delicious { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-digg { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pied-piper-pp { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pied-piper-alt { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-drupal { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-joomla { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-behance { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-behance-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-behance-square:before { + content: "\f1b5"; } + +.fa.fa-steam { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-steam-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-steam-square:before { + content: "\f1b7"; } + +.fa.fa-automobile:before { + content: "\f1b9"; } + +.fa.fa-cab:before { + content: "\f1ba"; } + +.fa.fa-spotify { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-deviantart { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-soundcloud { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-file-pdf-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-pdf-o:before { + content: "\f1c1"; } + +.fa.fa-file-word-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-word-o:before { + content: "\f1c2"; } + +.fa.fa-file-excel-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-excel-o:before { + content: "\f1c3"; } + +.fa.fa-file-powerpoint-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-powerpoint-o:before { + content: "\f1c4"; } + +.fa.fa-file-image-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-image-o:before { + content: "\f1c5"; } + +.fa.fa-file-photo-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-photo-o:before { + content: "\f1c5"; } + +.fa.fa-file-picture-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-picture-o:before { + content: "\f1c5"; } + +.fa.fa-file-archive-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-archive-o:before { + content: "\f1c6"; } + +.fa.fa-file-zip-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-zip-o:before { + content: "\f1c6"; } + +.fa.fa-file-audio-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-audio-o:before { + content: "\f1c7"; } + +.fa.fa-file-sound-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-sound-o:before { + content: "\f1c7"; } + +.fa.fa-file-video-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-video-o:before { + content: "\f1c8"; } + +.fa.fa-file-movie-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-movie-o:before { + content: "\f1c8"; } + +.fa.fa-file-code-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-code-o:before { + content: "\f1c9"; } + +.fa.fa-vine { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-codepen { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-jsfiddle { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-life-bouy:before { + content: "\f1cd"; } + +.fa.fa-life-buoy:before { + content: "\f1cd"; } + +.fa.fa-life-saver:before { + content: "\f1cd"; } + +.fa.fa-support:before { + content: "\f1cd"; } + +.fa.fa-circle-o-notch:before { + content: "\f1ce"; } + +.fa.fa-rebel { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-ra { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-ra:before { + content: "\f1d0"; } + +.fa.fa-resistance { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-resistance:before { + content: "\f1d0"; } + +.fa.fa-empire { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-ge { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-ge:before { + content: "\f1d1"; } + +.fa.fa-git-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-git-square:before { + content: "\f1d2"; } + +.fa.fa-git { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-hacker-news { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-y-combinator-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-y-combinator-square:before { + content: "\f1d4"; } + +.fa.fa-yc-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yc-square:before { + content: "\f1d4"; } + +.fa.fa-tencent-weibo { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-qq { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-weixin { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wechat { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wechat:before { + content: "\f1d7"; } + +.fa.fa-send:before { + content: "\f1d8"; } + +.fa.fa-paper-plane-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-paper-plane-o:before { + content: "\f1d8"; } + +.fa.fa-send-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-send-o:before { + content: "\f1d8"; } + +.fa.fa-circle-thin { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-circle-thin:before { + content: "\f111"; } + +.fa.fa-header:before { + content: "\f1dc"; } + +.fa.fa-futbol-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-futbol-o:before { + content: "\f1e3"; } + +.fa.fa-soccer-ball-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-soccer-ball-o:before { + content: "\f1e3"; } + +.fa.fa-slideshare { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-twitch { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yelp { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-newspaper-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-newspaper-o:before { + content: "\f1ea"; } + +.fa.fa-paypal { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-google-wallet { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-visa { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-mastercard { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-discover { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-amex { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-paypal { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-stripe { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bell-slash-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-bell-slash-o:before { + content: "\f1f6"; } + +.fa.fa-trash:before { + content: "\f2ed"; } + +.fa.fa-copyright { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-eyedropper:before { + content: "\f1fb"; } + +.fa.fa-area-chart:before { + content: "\f1fe"; } + +.fa.fa-pie-chart:before { + content: "\f200"; } + +.fa.fa-line-chart:before { + content: "\f201"; } + +.fa.fa-lastfm { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-lastfm-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-lastfm-square:before { + content: "\f203"; } + +.fa.fa-ioxhost { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-angellist { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-cc:before { + content: "\f20a"; } + +.fa.fa-ils:before { + content: "\f20b"; } + +.fa.fa-shekel:before { + content: "\f20b"; } + +.fa.fa-sheqel:before { + content: "\f20b"; } + +.fa.fa-buysellads { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-connectdevelop { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-dashcube { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-forumbee { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-leanpub { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-sellsy { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-shirtsinbulk { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-simplybuilt { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-skyatlas { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-diamond { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-diamond:before { + content: "\f3a5"; } + +.fa.fa-transgender:before { + content: "\f224"; } + +.fa.fa-intersex:before { + content: "\f224"; } + +.fa.fa-transgender-alt:before { + content: "\f225"; } + +.fa.fa-facebook-official { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-facebook-official:before { + content: "\f09a"; } + +.fa.fa-pinterest-p { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-whatsapp { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-hotel:before { + content: "\f236"; } + +.fa.fa-viacoin { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-medium { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-y-combinator { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yc { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yc:before { + content: "\f23b"; } + +.fa.fa-optin-monster { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-opencart { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-expeditedssl { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-battery-4:before { + content: "\f240"; } + +.fa.fa-battery:before { + content: "\f240"; } + +.fa.fa-battery-3:before { + content: "\f241"; } + +.fa.fa-battery-2:before { + content: "\f242"; } + 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+
+ + + + +
+ +
+
+
+

iUSEiSEE - An overview of iSEE

+

Federico +Marini1, +Najla Abassi2

+
+Collection of material developed by the iSEE core dev team
(Kevin +Rue-Albrecht, Federico Marini, Charlotte Soneson, Aaron Lun)

+ + + Source: vignettes/iSEE_overview_02.Rmd + + +
+ + + +

+
+

Introduction +

+

This vignette provides an overview of the graphical interface of +iSEE +applications (Rue-Albrecht et al. 2018). +To follow along, make sure that you launch the default iSEE +instance as described in the next code block at the start of the following section.

+

Note that in the default configuration, the panels do not look +exactly like the ones shown in the screenshots that you will see below. +For example, data points are not immediately colored, and the default +annotation variables displayed by each panel may differ. The hands-on recipes workshop of this workshop will +demonstrate how to modify the content of the panels, and how they are +displayed.

+

Note that for simplicity, we typically refer to a +SummarizedExperiment in this workshop; however, iSEE works +seamlessly for objects of any class extending +SummarizedExperiment as well (e.g., +SingleCellExperiment, DESeqDataSet). That +said, some types of panels – such as the Reduced dimension plot – are +only available for objects that contain a reducedDim slot +(in particular, SingleCellExperiment objects); the basic +SummarizedExperiment class does not contain this slot. In +this workshop, we refer to the rows of the +SummarizedExperiment object as ‘features’ (these can be +genes, transcripts, genomic regions, etc) and to the columns as +‘samples’ (which, in our example data set, are single cells).

+
+
+

The default iSEE app +

+

Using the demonstration data set, we can launch an iSEE +instance for exploring this data set using the iSEE() +function without any further argument. This will produce an app using +the default configuration; that is, the app instance will include one +panel of each built-in class for which the relevant information is +available in the SummarizedExperiment object.

+
+library("iSEE")
+sce_location <- system.file("datasets", "sce_pbmc3k.RDS", package = "iUSEiSEE")
+sce_location
+#> [1] "/Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/library/iUSEiSEE/datasets/sce_pbmc3k.RDS"
+sce <- readRDS(sce_location)
+app <- iSEE(sce)
+
+shiny::runApp(app)
+

+

The main argument to the iSEE() function is a +SummarizedExperiment object, or an object of any class +extending SummarizedExperiment (such as +SingleCellExperiment, in this case). No other restrictions +are made on the type of data stored in the object, and iSEE can be +used for interactive visualization of many different types of data. It +is also worth noting that for various types of data, Bioconductor +packages provides functionality for directly importing quantifications +generated by external software packages into a +SummarizedExperiment object. For example, the DropletUtils +package can read quantifications from the 10x Genomics CellRanger +pipeline for single-cell RNA-seq data, and the tximeta +package can be used to read data from transcript quantification +pipelines into a SummarizedExperiment object.

+

While we will not make explicit use of it in this workshop, we note +that it is also possible to call iSEE() without providing a +SummarizedExperiment object. In that case, the user will be +prompted to upload such an object, serialized into a .rds +file. It is also possible to import a specification of the initial panel +setup.

+
+app <- iSEE()
+
+shiny::runApp(app)
+

+
+
+

The built-in panel types +

+
+

The panel hierarchy +

+

The iSEE user +interface (UI) consists of a number of panels, each displaying the data +provided in the SummarizedExperiment from a specific +perspective. There are 8 standard panel types; 6 plot panels and 2 table +panels, all showcased in the figure shown at the start of the previous section. In the default configuration, +one panel of each type is included when launching the iSEE user +interface. However, users are free to rearrange, resize, remove or add +panels freely, as demonstrated in the separate vignette of workshop recipes. We provide a brief overview of +each panel type in the following subsections.

+

In addition to the 8 standard panel types included in iSEE, users +can create custom panels (both plots and tables). Moreover, the iSEEu (iSEE +universe) package contains additional panel types. The creation and +configuration of custom panels is also demonstrated in a series of workshop advanced recipes.

+

Specifically, iSEE panels +are implemented as a hierarchy of S4 classes. Only concrete +classes (orange) may be instantiated as panels in the user interface; +while virtual classes (grey) provide functionality shared by +families of panels (e.g., sample-oriented plots, feature-oriented +tables).

+

+ +
+
+

Reduced dimension plot +

+

The reduced dimension plot can display any reduced dimension +representation that is present in the reducedDim slot of +the SingleCellExperiment object.

+

Note that this slot is not defined for the base +SummarizedExperiment class, in which case the user +interface does not allow the inclusion of panels of this type.

+

+
+
+

Column data plot +

+

The column data plot can display one or two of the provided column +annotations (from the colData slot). Depending on the class +of the selected annotations, the panel shows either a scatter plot, a +violin plot, or a Hinton diagram (Hinton and +Shallice 1991; Bremner, Gotts, and Denham 1994).

+

+
+
+

Row data plot +

+

Analogous to the column data plot above, +the row data plot displays one or two of the provided row annotations +(from the rowData slot). Depending on the class of the +selected annotations, the panel displays either a scatter plot, a violin +plot, or a Hinton plot.

+

+
+
+

Complex heatmap +

+

The complex heatmap panel displays, for any assay, the observed +values for a subset of the features across the samples.

+

+
+
+

Feature assay plot +

+

The feature assay plot displays the observed values for one feature +across the samples. It is also possible to plot the observed values for +two features, in a scatter plot.

+

+
+
+

Sample assay plot +

+

Analogous to the Feature assay plot +above, the Sample assay plot shows the observed values for all features, +for one of the samples. It is also possible to plot the observed values +for two samples, in a scatter plot.

+

+
+
+

Row data table +

+

The row data table displays all information provided in the +rowData slot of the SummarizedExperiment +object, leveraging the interactivity provided by the DT package.

+

+
+
+

Column data table +

+

Analogous to the Row data table above, +the column data table displays all information provided in the +colData slot of the SummarizedExperiment +object.

+

+
+
+
+

Collapsible boxes with display controls +

+
+

Data parameters +

+

Each plot panel type has a Data parameters collapsible +box. This box has different content for each panel type, but in all +cases it lets the user control the data that is displayed in the +plot.

+

+
+
+

Visual parameters +

+

In contrast to the Data parameters collapsible box that +lets users control what is displayed in the plot, the +Visual parameters box lets users control how the +information is displayed.

+

This collapsible box contains the controls to change the size, shape, +opacity, and color of the points, to facet the plot by any available +categorical annotation, to subsample points for increased speed of plot +rendering, and to control how legends are displayed.

+

+
+
+

Selection parameters +

+

The Selection parameters collapsible box provides +controls to transfer selections of points (features or samples) between +panels.

+

We demonstrate examples of point transmission in the separate +vignette of workshop recipes

+

+
+
+
+

Additional controls +

+

At the top-right corner of the iSEE application, users can find +additional controls for reproducibility, configuration, and help.

+ +++++ + + + + + + + + + + + + + + + + + + + + + + +
Organization +Organize panels
Examine panel chart +
Export +Download panel output
Extract R code
Display panel settings +
Documentation +Quick tour
Open the vignette +
Additional info +About this session
About iSEE +
+
+

Organize panels +

+

As mentioned above, the default behaviour of the iSEE() +function is to launch an instance of the user interface that displays +one panel of each of the standard types (provided the underlying data is +available, e.g. for reduced dimension plots the reducedDim +slot is required). However, in some cases it is desirable to have +multiple panels of the same type, and/or exclude some panel types.

+

In order to accommodate such situations, users can add, remove, +change the order of and resize all panels via the +Organization menu in the top-right corner.

+

Clicking in the selectize box listing all current panels will present +you with a drop-down menu from which you can choose additional panels to +add. Similarly, panels can be removed by clicking on the + icon associated with the panel name.

+

Each panel can be individually resized by changing the width and +height. Note that the total width of a row in the interface is 12 units. +When the width of a panel is greater than the space available, the panel +is moved to a new row.

+

+
+
+

Linking panels and transmitting point selections +

+

When exploring data, it is often useful to be able to select a subset +of points and investigate different aspects of their characteristic +features. In iSEE, this +can be achieved by selecting points in one panel, and +transmitting this selection to one or more other panels.

+

The brushing and point selection can also be programmatically +preconfigured.

+

The button Examine panel chart display a graph that +reports any active point transmission between panels in the app.

+

+
+
+

Download panel output +

+

The Download panel output button opens a modal window +listing all the current panels in the app. Checkboxes allow users to +select any subset of panels to export. Finally, clicking the +Download button in that modal will prompt the app to save +plots to PDF files, tables to CSV files, package the set of files in a +ZIP archive that users can download and save on their computer.

+

+
+
+

Extract R code +

+

The fact that data exploration is done interactively is no reason to +forego reproducibility! To this end, iSEE lets +you export the exact R code used to create each of the currently visible +plots.

+

Importantly, the script reported by iSEE +contains a short preamble needed to set up variable names that are used +in individual panels, including active brushes used to transfer point +selections.

+

+
+
+

Display panel settings +

+

It can take a great amount of time to achieve a satisfactory panel +configuration. To avoid the need to manually organize the panels each +time the app is opened, iSEE offers +the possibility to export code that can be reused later to +programmatically specify how the app is initialized, as well as to +inspect and export the current panel settings for future use.

+

+
+
+

Quick tour +

+

One important aspect of visualization is the ability to share your +insights with others. A powerful way of easily getting people unfamiliar +with your data up to speed is to provide a walkthrough of the interface +and the different types of plots that are displayed. With iSEE, this +can be achieved using tours. To configure a tour, you need to +create a text file with two columns; named element and +intro, with the former describing the UI element to +highlight in each step, and the latter providing the descriptive text +that will be displayed.

+

Clicking the Quick tour button launches an interactive +tour of the interface using the rintrojs +package, highlighting specific elements of the user interface, labeled +with information and instructions guiding users through panels and tasks +specific to individual apps.

+

+

You can see a live example of a more complex tour in action, visit https://marionilab.cruk.cam.ac.uk/iSEE_pbmc4k/. The code +used to create this app and the associated tour is available from https://github.com/iSEE/iSEE2018/tree/master/tours/pbmc4k.

+
+
+

Open the vignette +

+

The user interface navigation bar also includes this button to open +the introductory vignette to iSEE in your +we browser.

+

Depending on the version of iSEE that +you are using, this will adaptively lead you to a locally built vignette +present on your computer, or the release or the devel +version of the Bioconductor package landing page, e.g. <Bioconductor +3.19>.

+
+
+

Session information +

+

This button displays the output of sessionInfo(), which +is a useful piece of information to report when reporting an issue with +an app.

+

+
+
+

About iSEE +

+

This button provides information about the authors of iSEE, as +well as citation information.

+

If you use this package, please use the following citation +information:

+
+

Rue-Albrecht K, Marini F, Soneson C, Lun ATL (2018). “iSEE: +Interactive SummarizedExperiment Explorer.” F1000Research, +7, 741. doi: 10.12688/f1000research.14966.1 (URL: https://doi.org/10.12688/f1000research.14966.1).

+
+

A BibTeX entry for LaTeX users is:

+
@Article{,
+  title = {iSEE: Interactive SummarizedExperiment Explorer},
+  author = {Kevin Rue-Albrecht and Federico Marini and Charlotte Soneson and Aaron T. L. Lun},
+  publisher = {F1000 Research, Ltd.},
+  journal = {F1000Research},
+  year = {2018},
+  month = {Jun},
+  volume = {7},
+  pages = {741},
+  doi = {10.12688/f1000research.14966.1},
+}
+
+
+
+

Using iSEE: what can you do with it? +

+

In this section, we list a number of actions you can perform, +possibly in a very smooth manner, with the help of iSEE.

+

These are reported here as bullet points, and will be demonstrated by +the instructors.

+
    +
  • Organize the iSEE panels: +
      +
    • Search for the Organize panels button.
      • +
    • Try to add and remove panels, and resize the existing ones.
      • +
    • Remember to click on Apply settings afterwards.
      • +
    • See how to export the initial configuration of +panels.
      • +
    • Relaunch the application, now with the additional argument +initial provided
      • +
    +
    • +
  • Seeing more: +
      +
    • Show two Reduced dimension plot panels; one showing the PCA +representation, one showing the t-SNE representation.
      • +
    • Link them together
      • +
    +
    • +
  • See the true colors of your data: +
      +
    • Open the Visual parameters collapsible box in one of +the Reduced dimension plot panels, and set +Color by: to Column data.
      • +
    • Color the cells by Cluster, which contains the cluster +labels that were assigned to the cells in the preprocessing step
      • +
    • Color the cells by the log10-transformed number of detected genes +(log10_total).#
      • +
    • Set Color by: to Feature name in one of +the Reduced dimension plot panels, and select one of the genes +from the dropdown menu. You can search for a gene of interest by typing +in the dropdown box.
      • +
    +
    • +
  • Highlighting & zooming: +
      +
    • Set Color by: to Sample name in one of the +Reduced dimension plots, and use the dropdown menu underneath to change +the highlighted cell.
      • +
    • Here, we reduce the point size and increase the transparency +(i.e. reduce the value for the alpha attribute).
      • +
    • We also apply a downsampling grid of 100 horizontal and vertical +bins
      • +
    • Double-click on the area selected in the first panel to zoom and +display the same view as the second panel.
      • +
    • Double click again anywhere in the panel to zoom out.
      • +
    +
    • +
  • Ready, steady, go: +
      +
    • Start iSEE in the default configuration
      • +
    • Set up one Column data plot and one Row data plot +panel
      • +
    • Start iSEE in an empty configuration
      • +
    +
    • +
  • iSEE links: +
      +
    • Start an instance with the default set of panels and explore the +different types of plots generated by the Feature assay plot panel using +the choices available for the two axes.
      • +
    • Select a set of points in one of the Feature assay plot +panels, by drawing a rectangle around them.
      • +
    • Then open the Selection parameters collapsible box of one +of the other Feature assay plot panels, and select the +Feature assay plot panel where you made the point selection +from the dropdown menu under Receive column selection +from.
      • +
    • Note how the points corresponding to the cells that you selected in +the first panel are highlighted in the receiving panel.
      • +
    • You can highlight the points in different ways by changing the +Selection effect.
      • +
    • iSEE can display active links transmitting point selections between +panel using the “Examine panel chart” +menu.
      • +
    +
    • +
  • Selecting things +
      +
    • Get ready to know the rectangular selection and the lasso +selection
      • +
    • Create multiple selections
      • +
    +
    • +
  • Let’s export things! +
      +
    • Click on the export icon () in +the top-right corner, and select ‘Display panel settings’.
      • +
    • Copy all the code shown in the pop-up window.
      • +
    • Close the app and paste this code in your R session.
      • +
    • This defines the ‘initial’ list that we have composed manually in +the examples above. Then launch a new instance using the following +code.
      • +
    • Note how the app starts in the same configuration as it was before +closing. Of course, it is still possible to continue exploring the data +interactively - we have only changed the starting configuration.
      • +
    • On to the code for the output now! Click on the export icon +() in the top-right corner, and +select ‘Extract the R code’.
      • +
    • Copy the preamble and the code for the first Reduced dimension +plot from the pop-up window. Close the app and paste the code in +your R session.
      • +
    • Note how this recreates the first reduced dimension plot from the +app.
      • +
    +
    • +
+
+
+

Modes and additional panels +

+

The iSEEu +(“iSEE universe”) Bioconductor package defines additional custom panels +and predefined ‘modes’ (startup configurations) that may be useful for +specific applications. Here we illustrate the use of the reduced +dimension mode, which will start an application with one reduced +dimension panel for each reduced dimension representation in the input +object.

+
+library("iSEEu")
+#> Loading required package: iSEEhex
+app <- modeReducedDim(sce)
+
+shiny::runApp(app)
+

+

Furthermore, since iSEE version +2.0.0, users can leverage the implementation of panels as a hierarchy of +S4 classes to rapidly extend the framework and develop new types of +panels with virtually unlimited freedom of functionality. In this +framework, new panel types can be readily integrated in apps alongside +built-in panel types and immediately benefit of most panel functionality +with minimal effort, including the capacity to transmit selections to +and from other panels. New panels may be implemented by extending the +core virtual class Panel directly for full control over the +user interface and reactive observers; alternatively, it is often +desirable for developers to inherit from one of the concrete panel +classes to get most of the essential functionality from the parent class +“for free”.

+

Use cases demonstrating the implementation of new panels with various +levels of complexity are available in the Extending iSEE bookdown. +For example, the Hexagonal reduced dimension plot - implemented +in the iSEEu +package - demonstrates an alternative to the downsampling strategy, by +summarizing data points into hexagonal bins.

+
+app <- iSEE(sce, initial = list(
+  ReducedDimensionPlot(PanelWidth = 6L),
+  ReducedDimensionHexPlot(PanelWidth = 6L)
+))
+
+shiny::runApp(app)
+

+

Another useful mode you can use with your data, especially if using +iSEE for mass cytometry data, would be the modeGating. This +launches an app preconfigured with multiple chain-linked feature +expression plots for interactive data exploration.

+
+# Select top variable genes ----
+plot_count <- 6
+rv <- rowVars(assay(sce, "logcounts"))
+top_var <- head(order(rv, decreasing=TRUE), plot_count*2)
+top_var_genes <- rownames(sce)[top_var]
+
+plot_features <- data.frame(
+  x=head(top_var_genes, plot_count),
+  y=tail(top_var_genes, plot_count),
+  stringsAsFactors=FALSE
+)
+
+# launch the app itself ----
+
+app <- modeGating(sce, 
+                  plotAssay = "logcounts",
+                  features = plot_features)
+
+shiny::runApp(app)
+

+

Interestingly, iSEEu also has a set of extra panels that can come in +handy in different situations. For example, +iSEEu::AggregatedDotPlot implements an aggregated dot plot +where each feature/group combination is represented by a dot. The color +of the dot scales with the mean assay value across all samples for a +given group, while the size of the dot scales with the proportion of +non-zero values across samples in that group. This can be an alternative +to a heatmap, and an example can be seen in the chunks below:

+
+app <- iSEE(
+  sce, 
+  initial = list(
+    AggregatedDotPlot(
+      Assay = "logcounts",
+      ColumnDataLabel = "labels_main",
+      CustomRowsText = top_var_genes
+    )
+    
+  )
+)
+

+

New panels and modes may be defined in independent R +packages that declare Imports: iSEE in their DESCRIPTION +file, and distributed online to create an “iSEE-verse” of interoperable +packages for interactive visualization, akin to the tidyverse +collection of R packages for data science. If you have ideas about +additional panels and/or modes that might be useful, we welcome issues +and pull requests at https://github.com/iSEE/iSEEu.

+
+
+

Additional resources +

+

A lot of materials have been developed, from the first use of iSEE to +contributions of new panel classes in separate downstream packages.

+ +
+
+

Session info +

+
+sessionInfo()
+#> R version 4.4.0 (2024-04-24)
+#> Platform: x86_64-apple-darwin20
+#> Running under: macOS Monterey 12.7.1
+#> 
+#> Matrix products: default
+#> BLAS:   /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRblas.0.dylib 
+#> LAPACK: /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRlapack.dylib;  LAPACK version 3.12.0
+#> 
+#> locale:
+#> [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
+#> 
+#> time zone: Europe/Berlin
+#> tzcode source: internal
+#> 
+#> attached base packages:
+#> [1] stats4    stats     graphics  grDevices utils     datasets  methods  
+#> [8] base     
+#> 
+#> other attached packages:
+#>  [1] iSEEu_1.15.1                iSEEhex_1.5.0              
+#>  [3] iSEE_2.15.1                 SingleCellExperiment_1.26.0
+#>  [5] SummarizedExperiment_1.33.3 Biobase_2.64.0             
+#>  [7] GenomicRanges_1.55.4        GenomeInfoDb_1.40.0        
+#>  [9] IRanges_2.38.0              S4Vectors_0.42.0           
+#> [11] BiocGenerics_0.50.0         MatrixGenerics_1.16.0      
+#> [13] matrixStats_1.3.0           BiocStyle_2.32.0           
+#> 
+#> loaded via a namespace (and not attached):
+#>   [1] bitops_1.0-7             rlang_1.1.3              magrittr_2.0.3          
+#>   [4] shinydashboard_0.7.2     clue_0.3-65              GetoptLong_1.0.5        
+#>   [7] compiler_4.4.0           mgcv_1.9-1               png_0.1-8               
+#>  [10] systemfonts_1.0.6        vctrs_0.6.5              pkgconfig_2.0.3         
+#>  [13] shape_1.4.6.1            crayon_1.5.2             fastmap_1.1.1           
+#>  [16] XVector_0.44.0           fontawesome_0.5.2        utf8_1.2.4              
+#>  [19] promises_1.3.0           rmarkdown_2.26           shinyAce_0.4.2          
+#>  [22] UCSC.utils_1.0.0         ragg_1.3.0               purrr_1.0.2             
+#>  [25] xfun_0.43                zlibbioc_1.50.0          cachem_1.0.8            
+#>  [28] jsonlite_1.8.8           listviewer_4.0.0         highr_0.10              
+#>  [31] later_1.3.2              DelayedArray_0.30.0      parallel_4.4.0          
+#>  [34] cluster_2.1.6            R6_2.5.1                 bslib_0.7.0             
+#>  [37] RColorBrewer_1.1-3       jquerylib_0.1.4          Rcpp_1.0.12             
+#>  [40] iterators_1.0.14         knitr_1.46               httpuv_1.6.15           
+#>  [43] Matrix_1.7-0             splines_4.4.0            igraph_2.0.3            
+#>  [46] tidyselect_1.2.1         rstudioapi_0.16.0        abind_1.4-5             
+#>  [49] yaml_2.3.8               doParallel_1.0.17        codetools_0.2-20        
+#>  [52] miniUI_0.1.1.1           lattice_0.22-6           tibble_3.2.1            
+#>  [55] shiny_1.8.1.1            evaluate_0.23            desc_1.4.3              
+#>  [58] circlize_0.4.16          pillar_1.9.0             BiocManager_1.30.22     
+#>  [61] DT_0.33                  foreach_1.5.2            shinyjs_2.1.0           
+#>  [64] generics_0.1.3           RCurl_1.98-1.14          ggplot2_3.5.1           
+#>  [67] sparseMatrixStats_1.16.0 munsell_0.5.1            scales_1.3.0            
+#>  [70] xtable_1.8-4             glue_1.7.0               tools_4.4.0             
+#>  [73] hexbin_1.28.3            colourpicker_1.3.0       fs_1.6.4                
+#>  [76] grid_4.4.0               colorspace_2.1-0         nlme_3.1-164            
+#>  [79] GenomeInfoDbData_1.2.12  vipor_0.4.7              cli_3.6.2               
+#>  [82] textshaping_0.3.7        fansi_1.0.6              viridisLite_0.4.2       
+#>  [85] S4Arrays_1.4.0           ComplexHeatmap_2.20.0    dplyr_1.1.4             
+#>  [88] gtable_0.3.5             rintrojs_0.3.4           sass_0.4.9              
+#>  [91] digest_0.6.35            SparseArray_1.4.0        ggrepel_0.9.5           
+#>  [94] rjson_0.2.21             htmlwidgets_1.6.4        memoise_2.0.1           
+#>  [97] htmltools_0.5.8.1        pkgdown_2.0.9            lifecycle_1.0.4         
+#> [100] shinyWidgets_0.8.6       httr_1.4.7               GlobalOptions_0.1.2     
+#> [103] mime_0.12
+
+
+

References +

+
+
+Bremner, Frederick J, Stephen J Gotts, and Dina L Denham. 1994. +“Hinton Diagrams: Viewing Connection Strengths in Neural +Networks.” Behav. Res. Methods Instrum. Comput. 26 (2): +215–18. +
+
+Hinton, Geoffrey E, and Tim Shallice. 1991. “Lesioning an +Attractor Network: Investigations of Acquired Dyslexia.” +Psychological Review 98 (1): 74–95. +
+
+Rue-Albrecht, Kevin, Federico Marini, Charlotte Soneson, and Aaron T L +Lun. 2018. “iSEE: Interactive +SummarizedExperiment Explorer.” F1000Res. 7 +(June). +
+
+
+
+
+
    +

  1. ↩︎

    2. +

  2. ↩︎

    3. +
+
+
+ + + +
+ + + + +
+ + + + + + + + diff --git a/articles/iSEE_overview_02_files/font-awesome-6.4.2/css/all.css b/articles/iSEE_overview_02_files/font-awesome-6.4.2/css/all.css new file mode 100644 index 0000000..bdb6e3a --- /dev/null +++ b/articles/iSEE_overview_02_files/font-awesome-6.4.2/css/all.css @@ -0,0 +1,7968 @@ +/*! + * Font Awesome Free 6.4.2 by @fontawesome - https://fontawesome.com + * License - https://fontawesome.com/license/free (Icons: CC BY 4.0, Fonts: SIL OFL 1.1, Code: MIT License) + * Copyright 2023 Fonticons, Inc. + */ +.fa { + font-family: var(--fa-style-family, "Font Awesome 6 Free"); + font-weight: var(--fa-style, 900); } + +.fa, +.fa-classic, +.fa-sharp, +.fas, +.fa-solid, +.far, +.fa-regular, +.fab, +.fa-brands { + -moz-osx-font-smoothing: grayscale; + -webkit-font-smoothing: antialiased; + display: var(--fa-display, inline-block); + font-style: normal; + font-variant: normal; + line-height: 1; + text-rendering: auto; } + +.fas, +.fa-classic, +.fa-solid, +.far, +.fa-regular { + font-family: 'Font Awesome 6 Free'; } + +.fab, +.fa-brands { + font-family: 'Font Awesome 6 Brands'; } + +.fa-1x { + font-size: 1em; } + +.fa-2x { + font-size: 2em; } + +.fa-3x { + font-size: 3em; } + +.fa-4x { + font-size: 4em; } + +.fa-5x { + font-size: 5em; } + +.fa-6x { + font-size: 6em; } + +.fa-7x { + font-size: 7em; } + +.fa-8x { + font-size: 8em; } + +.fa-9x { + font-size: 9em; } + +.fa-10x { + font-size: 10em; } + +.fa-2xs { + font-size: 0.625em; + line-height: 0.1em; + vertical-align: 0.225em; } + +.fa-xs { + font-size: 0.75em; + line-height: 0.08333em; + vertical-align: 0.125em; } + +.fa-sm { + font-size: 0.875em; + line-height: 0.07143em; + vertical-align: 0.05357em; } + +.fa-lg { + font-size: 1.25em; + line-height: 0.05em; + vertical-align: -0.075em; } + +.fa-xl { + font-size: 1.5em; + line-height: 0.04167em; + vertical-align: -0.125em; } + +.fa-2xl { + font-size: 2em; + line-height: 0.03125em; + vertical-align: -0.1875em; } + +.fa-fw { + text-align: center; + width: 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var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, cubic-bezier(0.28, 0.84, 0.42, 1)); + animation-timing-function: var(--fa-animation-timing, cubic-bezier(0.28, 0.84, 0.42, 1)); } + +.fa-fade { + -webkit-animation-name: fa-fade; + animation-name: fa-fade; + -webkit-animation-delay: var(--fa-animation-delay, 0s); + animation-delay: var(--fa-animation-delay, 0s); + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 1s); + animation-duration: var(--fa-animation-duration, 1s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, cubic-bezier(0.4, 0, 0.6, 1)); + animation-timing-function: var(--fa-animation-timing, cubic-bezier(0.4, 0, 0.6, 1)); } + +.fa-beat-fade { + -webkit-animation-name: fa-beat-fade; + animation-name: fa-beat-fade; + -webkit-animation-delay: var(--fa-animation-delay, 0s); + animation-delay: var(--fa-animation-delay, 0s); + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 1s); + animation-duration: var(--fa-animation-duration, 1s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, cubic-bezier(0.4, 0, 0.6, 1)); + animation-timing-function: var(--fa-animation-timing, cubic-bezier(0.4, 0, 0.6, 1)); } + +.fa-flip { + -webkit-animation-name: fa-flip; + animation-name: fa-flip; + -webkit-animation-delay: var(--fa-animation-delay, 0s); + animation-delay: var(--fa-animation-delay, 0s); + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 1s); + animation-duration: var(--fa-animation-duration, 1s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, ease-in-out); + animation-timing-function: var(--fa-animation-timing, ease-in-out); } + +.fa-shake { + -webkit-animation-name: fa-shake; + animation-name: fa-shake; + -webkit-animation-delay: var(--fa-animation-delay, 0s); + animation-delay: var(--fa-animation-delay, 0s); + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 1s); + animation-duration: var(--fa-animation-duration, 1s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, linear); + animation-timing-function: var(--fa-animation-timing, linear); } + +.fa-spin { + -webkit-animation-name: fa-spin; + animation-name: fa-spin; + -webkit-animation-delay: var(--fa-animation-delay, 0s); + animation-delay: var(--fa-animation-delay, 0s); + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 2s); + animation-duration: var(--fa-animation-duration, 2s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, linear); + animation-timing-function: var(--fa-animation-timing, linear); } + +.fa-spin-reverse { + --fa-animation-direction: reverse; } + +.fa-pulse, +.fa-spin-pulse { + -webkit-animation-name: fa-spin; + animation-name: fa-spin; + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 1s); + animation-duration: var(--fa-animation-duration, 1s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, steps(8)); + animation-timing-function: var(--fa-animation-timing, steps(8)); } + +@media (prefers-reduced-motion: reduce) { + .fa-beat, + .fa-bounce, + .fa-fade, + .fa-beat-fade, + .fa-flip, + .fa-pulse, + .fa-shake, + .fa-spin, + .fa-spin-pulse { + -webkit-animation-delay: -1ms; + animation-delay: -1ms; + -webkit-animation-duration: 1ms; + animation-duration: 1ms; + -webkit-animation-iteration-count: 1; + animation-iteration-count: 1; + -webkit-transition-delay: 0s; + transition-delay: 0s; + -webkit-transition-duration: 0s; + transition-duration: 0s; } } + +@-webkit-keyframes fa-beat { + 0%, 90% { + -webkit-transform: scale(1); + transform: scale(1); } + 45% { + -webkit-transform: scale(var(--fa-beat-scale, 1.25)); + transform: scale(var(--fa-beat-scale, 1.25)); } } + +@keyframes fa-beat { + 0%, 90% { + -webkit-transform: scale(1); + transform: scale(1); } + 45% { + -webkit-transform: scale(var(--fa-beat-scale, 1.25)); + transform: scale(var(--fa-beat-scale, 1.25)); } } + +@-webkit-keyframes fa-bounce { + 0% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } + 10% { + -webkit-transform: scale(var(--fa-bounce-start-scale-x, 1.1), var(--fa-bounce-start-scale-y, 0.9)) translateY(0); + transform: scale(var(--fa-bounce-start-scale-x, 1.1), var(--fa-bounce-start-scale-y, 0.9)) translateY(0); } + 30% { + -webkit-transform: scale(var(--fa-bounce-jump-scale-x, 0.9), var(--fa-bounce-jump-scale-y, 1.1)) translateY(var(--fa-bounce-height, -0.5em)); + transform: scale(var(--fa-bounce-jump-scale-x, 0.9), var(--fa-bounce-jump-scale-y, 1.1)) translateY(var(--fa-bounce-height, -0.5em)); } + 50% { + -webkit-transform: scale(var(--fa-bounce-land-scale-x, 1.05), var(--fa-bounce-land-scale-y, 0.95)) translateY(0); + transform: scale(var(--fa-bounce-land-scale-x, 1.05), var(--fa-bounce-land-scale-y, 0.95)) translateY(0); } + 57% { + -webkit-transform: scale(1, 1) translateY(var(--fa-bounce-rebound, -0.125em)); + transform: scale(1, 1) translateY(var(--fa-bounce-rebound, -0.125em)); } + 64% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } + 100% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } } + +@keyframes fa-bounce { + 0% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } + 10% { + -webkit-transform: scale(var(--fa-bounce-start-scale-x, 1.1), var(--fa-bounce-start-scale-y, 0.9)) translateY(0); + transform: scale(var(--fa-bounce-start-scale-x, 1.1), var(--fa-bounce-start-scale-y, 0.9)) translateY(0); } + 30% { + -webkit-transform: scale(var(--fa-bounce-jump-scale-x, 0.9), var(--fa-bounce-jump-scale-y, 1.1)) translateY(var(--fa-bounce-height, -0.5em)); + transform: scale(var(--fa-bounce-jump-scale-x, 0.9), var(--fa-bounce-jump-scale-y, 1.1)) translateY(var(--fa-bounce-height, -0.5em)); } + 50% { + -webkit-transform: scale(var(--fa-bounce-land-scale-x, 1.05), var(--fa-bounce-land-scale-y, 0.95)) translateY(0); + transform: scale(var(--fa-bounce-land-scale-x, 1.05), var(--fa-bounce-land-scale-y, 0.95)) translateY(0); } + 57% { + -webkit-transform: scale(1, 1) translateY(var(--fa-bounce-rebound, -0.125em)); + transform: scale(1, 1) translateY(var(--fa-bounce-rebound, -0.125em)); } + 64% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } + 100% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } } + +@-webkit-keyframes fa-fade { + 50% { + opacity: var(--fa-fade-opacity, 0.4); } } + +@keyframes fa-fade { + 50% { + opacity: var(--fa-fade-opacity, 0.4); } } + +@-webkit-keyframes fa-beat-fade { + 0%, 100% { + opacity: var(--fa-beat-fade-opacity, 0.4); + -webkit-transform: scale(1); + transform: scale(1); } + 50% { + opacity: 1; + -webkit-transform: scale(var(--fa-beat-fade-scale, 1.125)); + transform: scale(var(--fa-beat-fade-scale, 1.125)); } } + +@keyframes fa-beat-fade { + 0%, 100% { + opacity: var(--fa-beat-fade-opacity, 0.4); + -webkit-transform: scale(1); + transform: scale(1); } + 50% { + opacity: 1; + -webkit-transform: scale(var(--fa-beat-fade-scale, 1.125)); + transform: scale(var(--fa-beat-fade-scale, 1.125)); } } + +@-webkit-keyframes fa-flip { + 50% { + -webkit-transform: rotate3d(var(--fa-flip-x, 0), var(--fa-flip-y, 1), var(--fa-flip-z, 0), var(--fa-flip-angle, -180deg)); + transform: rotate3d(var(--fa-flip-x, 0), var(--fa-flip-y, 1), var(--fa-flip-z, 0), var(--fa-flip-angle, -180deg)); } } + +@keyframes fa-flip { + 50% { + -webkit-transform: rotate3d(var(--fa-flip-x, 0), var(--fa-flip-y, 1), var(--fa-flip-z, 0), var(--fa-flip-angle, -180deg)); + transform: rotate3d(var(--fa-flip-x, 0), var(--fa-flip-y, 1), var(--fa-flip-z, 0), var(--fa-flip-angle, -180deg)); } } + +@-webkit-keyframes fa-shake { + 0% { + -webkit-transform: rotate(-15deg); + transform: rotate(-15deg); } + 4% { + -webkit-transform: rotate(15deg); + transform: rotate(15deg); } + 8%, 24% { + -webkit-transform: rotate(-18deg); + transform: rotate(-18deg); } + 12%, 28% { + -webkit-transform: rotate(18deg); + transform: rotate(18deg); } + 16% { + -webkit-transform: rotate(-22deg); + transform: rotate(-22deg); } + 20% { + -webkit-transform: rotate(22deg); + transform: rotate(22deg); } + 32% { + -webkit-transform: rotate(-12deg); + transform: rotate(-12deg); } + 36% { + -webkit-transform: rotate(12deg); + transform: rotate(12deg); } + 40%, 100% { + -webkit-transform: rotate(0deg); + transform: rotate(0deg); } } + +@keyframes fa-shake { + 0% { + -webkit-transform: rotate(-15deg); + transform: rotate(-15deg); } + 4% { + -webkit-transform: rotate(15deg); + transform: rotate(15deg); } + 8%, 24% { + -webkit-transform: rotate(-18deg); + transform: rotate(-18deg); } + 12%, 28% { + -webkit-transform: rotate(18deg); + transform: rotate(18deg); } + 16% { + -webkit-transform: rotate(-22deg); + transform: rotate(-22deg); } + 20% { + -webkit-transform: rotate(22deg); + transform: rotate(22deg); } + 32% { + -webkit-transform: rotate(-12deg); + transform: rotate(-12deg); } + 36% { + -webkit-transform: rotate(12deg); + transform: rotate(12deg); } + 40%, 100% { + -webkit-transform: rotate(0deg); + transform: rotate(0deg); } } + +@-webkit-keyframes fa-spin { + 0% { + -webkit-transform: rotate(0deg); + transform: rotate(0deg); } + 100% { + -webkit-transform: rotate(360deg); + transform: rotate(360deg); } } + +@keyframes fa-spin { + 0% { + -webkit-transform: rotate(0deg); + transform: rotate(0deg); } + 100% { + -webkit-transform: rotate(360deg); + transform: rotate(360deg); } } + +.fa-rotate-90 { + -webkit-transform: rotate(90deg); + transform: rotate(90deg); } + +.fa-rotate-180 { + -webkit-transform: rotate(180deg); + transform: rotate(180deg); } + +.fa-rotate-270 { + -webkit-transform: rotate(270deg); + transform: rotate(270deg); } + +.fa-flip-horizontal { + -webkit-transform: scale(-1, 1); + transform: scale(-1, 1); } + +.fa-flip-vertical { + -webkit-transform: scale(1, -1); + transform: scale(1, -1); } + +.fa-flip-both, +.fa-flip-horizontal.fa-flip-vertical { + -webkit-transform: scale(-1, -1); + transform: scale(-1, -1); } + +.fa-rotate-by { + -webkit-transform: rotate(var(--fa-rotate-angle, none)); + transform: rotate(var(--fa-rotate-angle, none)); } + +.fa-stack { + display: inline-block; + height: 2em; + line-height: 2em; + position: relative; + vertical-align: middle; + width: 2.5em; } + +.fa-stack-1x, +.fa-stack-2x { + left: 0; + position: absolute; + text-align: center; + width: 100%; + z-index: var(--fa-stack-z-index, auto); } + +.fa-stack-1x { + line-height: inherit; } + +.fa-stack-2x { + font-size: 2em; } + +.fa-inverse { + color: var(--fa-inverse, #fff); } + +/* Font Awesome uses the Unicode Private Use Area (PUA) to ensure screen +readers do not read off random characters that represent icons */ + +.fa-0::before { + content: "\30"; } + +.fa-1::before { + content: "\31"; } + +.fa-2::before { + content: "\32"; } + +.fa-3::before { + content: "\33"; } + +.fa-4::before { + content: "\34"; } + +.fa-5::before { + content: "\35"; } + +.fa-6::before { + content: "\36"; } + +.fa-7::before { + content: "\37"; } + +.fa-8::before { + content: "\38"; } + +.fa-9::before { + content: "\39"; } + +.fa-fill-drip::before { + content: "\f576"; } + +.fa-arrows-to-circle::before { + content: "\e4bd"; } + +.fa-circle-chevron-right::before { + content: "\f138"; } + +.fa-chevron-circle-right::before { + content: "\f138"; } + +.fa-at::before { + content: "\40"; } + +.fa-trash-can::before { + content: "\f2ed"; } + +.fa-trash-alt::before { + content: "\f2ed"; } + +.fa-text-height::before { + content: "\f034"; } + +.fa-user-xmark::before { + content: "\f235"; } + +.fa-user-times::before { + content: "\f235"; } + +.fa-stethoscope::before { + content: "\f0f1"; } + +.fa-message::before { + content: "\f27a"; } + +.fa-comment-alt::before { + content: "\f27a"; } + +.fa-info::before { + content: "\f129"; } + +.fa-down-left-and-up-right-to-center::before { + content: "\f422"; } + +.fa-compress-alt::before { + content: "\f422"; } + +.fa-explosion::before { + content: "\e4e9"; } + +.fa-file-lines::before { + content: "\f15c"; } + +.fa-file-alt::before { + content: "\f15c"; } + +.fa-file-text::before { + content: "\f15c"; } + +.fa-wave-square::before { + content: "\f83e"; } + +.fa-ring::before { + content: "\f70b"; } + +.fa-building-un::before { + content: "\e4d9"; } + +.fa-dice-three::before { + content: "\f527"; } + +.fa-calendar-days::before { + content: "\f073"; } + +.fa-calendar-alt::before { + content: "\f073"; } + +.fa-anchor-circle-check::before { + content: "\e4aa"; } + +.fa-building-circle-arrow-right::before { + content: "\e4d1"; } + +.fa-volleyball::before { + content: "\f45f"; } + +.fa-volleyball-ball::before { + content: "\f45f"; } + +.fa-arrows-up-to-line::before { + content: "\e4c2"; } + +.fa-sort-down::before { + content: "\f0dd"; } + +.fa-sort-desc::before { + content: "\f0dd"; } + +.fa-circle-minus::before { + content: "\f056"; } + +.fa-minus-circle::before { + content: "\f056"; } + +.fa-door-open::before { + content: "\f52b"; } + +.fa-right-from-bracket::before { + content: "\f2f5"; } + +.fa-sign-out-alt::before { + content: "\f2f5"; } + +.fa-atom::before { + content: "\f5d2"; } + +.fa-soap::before { + content: "\e06e"; } + +.fa-icons::before { + content: "\f86d"; } + +.fa-heart-music-camera-bolt::before { + content: "\f86d"; } + +.fa-microphone-lines-slash::before { + content: "\f539"; } + +.fa-microphone-alt-slash::before { + content: "\f539"; } + +.fa-bridge-circle-check::before { + content: "\e4c9"; } + +.fa-pump-medical::before { + content: "\e06a"; } + +.fa-fingerprint::before { + content: "\f577"; } + +.fa-hand-point-right::before { + content: "\f0a4"; } + +.fa-magnifying-glass-location::before { + content: "\f689"; } + +.fa-search-location::before { + content: "\f689"; } + +.fa-forward-step::before { + content: "\f051"; } + +.fa-step-forward::before { + content: "\f051"; } + +.fa-face-smile-beam::before { + content: "\f5b8"; } + +.fa-smile-beam::before { + content: "\f5b8"; } + +.fa-flag-checkered::before { + content: "\f11e"; } + +.fa-football::before { + content: "\f44e"; } + +.fa-football-ball::before { + content: "\f44e"; } + +.fa-school-circle-exclamation::before { + content: "\e56c"; } + +.fa-crop::before { + content: "\f125"; } + +.fa-angles-down::before { + content: "\f103"; } + +.fa-angle-double-down::before { + content: "\f103"; } + +.fa-users-rectangle::before { + content: "\e594"; } + +.fa-people-roof::before { + content: "\e537"; } + +.fa-people-line::before { + content: "\e534"; } + +.fa-beer-mug-empty::before { + content: "\f0fc"; } + +.fa-beer::before { + content: "\f0fc"; } + +.fa-diagram-predecessor::before { + content: "\e477"; } + +.fa-arrow-up-long::before { + content: "\f176"; } + +.fa-long-arrow-up::before { + content: "\f176"; } + +.fa-fire-flame-simple::before { + content: "\f46a"; } + +.fa-burn::before { + content: "\f46a"; } + +.fa-person::before { + content: "\f183"; } + +.fa-male::before { + content: "\f183"; } + +.fa-laptop::before { + content: "\f109"; } + +.fa-file-csv::before { + content: "\f6dd"; } + +.fa-menorah::before { + content: "\f676"; } + +.fa-truck-plane::before { + content: "\e58f"; } + +.fa-record-vinyl::before { + content: "\f8d9"; } + +.fa-face-grin-stars::before { + content: "\f587"; } + +.fa-grin-stars::before { + content: "\f587"; } + +.fa-bong::before { + content: "\f55c"; } + +.fa-spaghetti-monster-flying::before { + content: "\f67b"; } + +.fa-pastafarianism::before { + content: "\f67b"; } + +.fa-arrow-down-up-across-line::before { + content: "\e4af"; } + +.fa-spoon::before { + content: "\f2e5"; } + +.fa-utensil-spoon::before { + content: "\f2e5"; } + +.fa-jar-wheat::before { + content: "\e517"; } + +.fa-envelopes-bulk::before { + content: "\f674"; } + +.fa-mail-bulk::before { + content: "\f674"; } + +.fa-file-circle-exclamation::before { + content: "\e4eb"; } + +.fa-circle-h::before { + content: "\f47e"; } + +.fa-hospital-symbol::before { + content: "\f47e"; } + +.fa-pager::before { + content: "\f815"; } + +.fa-address-book::before { + content: "\f2b9"; } + +.fa-contact-book::before { + content: "\f2b9"; } + +.fa-strikethrough::before { + content: "\f0cc"; } + +.fa-k::before { + content: "\4b"; } + +.fa-landmark-flag::before { + content: "\e51c"; } + +.fa-pencil::before { + content: "\f303"; } + +.fa-pencil-alt::before { + content: "\f303"; } + +.fa-backward::before { + content: "\f04a"; } + +.fa-caret-right::before { + content: "\f0da"; } + +.fa-comments::before { + content: "\f086"; } + +.fa-paste::before { + content: "\f0ea"; } + +.fa-file-clipboard::before { + content: "\f0ea"; } + +.fa-code-pull-request::before { + content: "\e13c"; } + +.fa-clipboard-list::before { + content: "\f46d"; } + +.fa-truck-ramp-box::before { + content: "\f4de"; } + +.fa-truck-loading::before { + content: "\f4de"; } + +.fa-user-check::before { + content: "\f4fc"; } + +.fa-vial-virus::before { + content: "\e597"; } + +.fa-sheet-plastic::before { + content: "\e571"; } + +.fa-blog::before { + content: "\f781"; } + +.fa-user-ninja::before { + content: "\f504"; } + +.fa-person-arrow-up-from-line::before { + content: "\e539"; } + +.fa-scroll-torah::before { + content: "\f6a0"; } + +.fa-torah::before { + content: "\f6a0"; } + +.fa-broom-ball::before { + content: "\f458"; } + +.fa-quidditch::before { + content: "\f458"; } + +.fa-quidditch-broom-ball::before { + content: "\f458"; } + +.fa-toggle-off::before { + content: "\f204"; } + +.fa-box-archive::before { + content: "\f187"; } + +.fa-archive::before { + content: "\f187"; } + +.fa-person-drowning::before { + content: "\e545"; } + +.fa-arrow-down-9-1::before { + content: "\f886"; } + +.fa-sort-numeric-desc::before { + content: "\f886"; } + +.fa-sort-numeric-down-alt::before { + content: "\f886"; } + +.fa-face-grin-tongue-squint::before { + content: "\f58a"; } + +.fa-grin-tongue-squint::before { + content: "\f58a"; } + +.fa-spray-can::before { + content: "\f5bd"; } + +.fa-truck-monster::before { + content: "\f63b"; } + +.fa-w::before { + content: "\57"; } + +.fa-earth-africa::before { + content: "\f57c"; } + +.fa-globe-africa::before { + content: "\f57c"; } + +.fa-rainbow::before { + content: "\f75b"; } + +.fa-circle-notch::before { + content: "\f1ce"; } + +.fa-tablet-screen-button::before { + content: "\f3fa"; } + +.fa-tablet-alt::before { + content: "\f3fa"; } + +.fa-paw::before { + content: "\f1b0"; } + +.fa-cloud::before { + content: "\f0c2"; } + +.fa-trowel-bricks::before { + content: "\e58a"; } + +.fa-face-flushed::before { + content: "\f579"; } + +.fa-flushed::before { + content: "\f579"; } + +.fa-hospital-user::before { + content: "\f80d"; } + +.fa-tent-arrow-left-right::before { + content: "\e57f"; } + +.fa-gavel::before { + content: "\f0e3"; } + +.fa-legal::before { + content: "\f0e3"; } + +.fa-binoculars::before { + content: "\f1e5"; } + +.fa-microphone-slash::before { + content: "\f131"; } + +.fa-box-tissue::before { + content: "\e05b"; } + +.fa-motorcycle::before { + content: "\f21c"; } + +.fa-bell-concierge::before { + content: "\f562"; } + +.fa-concierge-bell::before { + content: "\f562"; } + +.fa-pen-ruler::before { + content: "\f5ae"; } + +.fa-pencil-ruler::before { + content: "\f5ae"; } + +.fa-people-arrows::before { + content: "\e068"; } + +.fa-people-arrows-left-right::before { + content: "\e068"; } + +.fa-mars-and-venus-burst::before { + content: "\e523"; } + +.fa-square-caret-right::before { + content: "\f152"; } + +.fa-caret-square-right::before { + content: "\f152"; } + +.fa-scissors::before { + content: "\f0c4"; } + +.fa-cut::before { + content: "\f0c4"; } + +.fa-sun-plant-wilt::before { + content: "\e57a"; } + +.fa-toilets-portable::before { + content: "\e584"; } + +.fa-hockey-puck::before { + content: "\f453"; } + +.fa-table::before { + content: "\f0ce"; } + +.fa-magnifying-glass-arrow-right::before { + content: "\e521"; } + +.fa-tachograph-digital::before { + content: "\f566"; } + +.fa-digital-tachograph::before { + content: "\f566"; } + +.fa-users-slash::before { + content: "\e073"; } + +.fa-clover::before { + content: "\e139"; } + +.fa-reply::before { + content: "\f3e5"; } + +.fa-mail-reply::before { + content: "\f3e5"; } + +.fa-star-and-crescent::before { + content: "\f699"; } + +.fa-house-fire::before { + content: "\e50c"; } + +.fa-square-minus::before { + content: "\f146"; } + +.fa-minus-square::before { + content: "\f146"; } + +.fa-helicopter::before { + content: "\f533"; } + +.fa-compass::before { + content: "\f14e"; } + +.fa-square-caret-down::before { + content: "\f150"; } + +.fa-caret-square-down::before { + content: "\f150"; } + +.fa-file-circle-question::before { + content: "\e4ef"; } + +.fa-laptop-code::before { + content: "\f5fc"; } + +.fa-swatchbook::before { + content: "\f5c3"; } + +.fa-prescription-bottle::before { + content: "\f485"; } + +.fa-bars::before { + content: "\f0c9"; } + +.fa-navicon::before { + content: "\f0c9"; } + +.fa-people-group::before { + content: "\e533"; } + +.fa-hourglass-end::before { + content: "\f253"; } + +.fa-hourglass-3::before { + content: "\f253"; } + +.fa-heart-crack::before { + content: "\f7a9"; } + +.fa-heart-broken::before { + content: "\f7a9"; } + +.fa-square-up-right::before { + content: "\f360"; } + +.fa-external-link-square-alt::before { + content: "\f360"; } + +.fa-face-kiss-beam::before { + content: "\f597"; } + +.fa-kiss-beam::before { + content: "\f597"; } + +.fa-film::before { + content: "\f008"; } + +.fa-ruler-horizontal::before { + content: "\f547"; } + +.fa-people-robbery::before { + content: "\e536"; } + +.fa-lightbulb::before { + content: "\f0eb"; } + +.fa-caret-left::before { + content: "\f0d9"; } + +.fa-circle-exclamation::before { + content: "\f06a"; } + +.fa-exclamation-circle::before { + content: "\f06a"; } + +.fa-school-circle-xmark::before { + content: "\e56d"; } + +.fa-arrow-right-from-bracket::before { + content: "\f08b"; } + +.fa-sign-out::before { + content: "\f08b"; } + +.fa-circle-chevron-down::before { + content: "\f13a"; } + +.fa-chevron-circle-down::before { + content: "\f13a"; } + +.fa-unlock-keyhole::before { + content: "\f13e"; } + +.fa-unlock-alt::before { + content: "\f13e"; } + +.fa-cloud-showers-heavy::before { + content: "\f740"; } + +.fa-headphones-simple::before { + content: "\f58f"; } + +.fa-headphones-alt::before { + content: "\f58f"; } + +.fa-sitemap::before { + content: "\f0e8"; } + +.fa-circle-dollar-to-slot::before { + content: "\f4b9"; } + +.fa-donate::before { + content: "\f4b9"; } + +.fa-memory::before { + content: "\f538"; } + +.fa-road-spikes::before { + content: "\e568"; } + +.fa-fire-burner::before { + content: "\e4f1"; } + +.fa-flag::before { + content: "\f024"; } + +.fa-hanukiah::before { + content: "\f6e6"; } + +.fa-feather::before { + content: "\f52d"; } + +.fa-volume-low::before { + content: "\f027"; } + +.fa-volume-down::before { + content: "\f027"; } + +.fa-comment-slash::before { + content: "\f4b3"; } + +.fa-cloud-sun-rain::before { + content: "\f743"; } + +.fa-compress::before { + content: "\f066"; } + +.fa-wheat-awn::before { + content: "\e2cd"; } + +.fa-wheat-alt::before { + content: "\e2cd"; } + +.fa-ankh::before { + content: "\f644"; } + +.fa-hands-holding-child::before { + content: "\e4fa"; } + +.fa-asterisk::before { + content: "\2a"; } + +.fa-square-check::before { + content: "\f14a"; } + +.fa-check-square::before { + content: "\f14a"; } + +.fa-peseta-sign::before { + content: "\e221"; } + +.fa-heading::before { + content: "\f1dc"; } + +.fa-header::before { + content: "\f1dc"; } + +.fa-ghost::before { + content: "\f6e2"; } + +.fa-list::before { + content: "\f03a"; } + +.fa-list-squares::before { + content: "\f03a"; } + +.fa-square-phone-flip::before { + content: "\f87b"; } + +.fa-phone-square-alt::before { + content: "\f87b"; } + +.fa-cart-plus::before { + content: "\f217"; } + +.fa-gamepad::before { + content: "\f11b"; } + +.fa-circle-dot::before { + content: "\f192"; } + +.fa-dot-circle::before { + content: "\f192"; } + +.fa-face-dizzy::before { + content: "\f567"; } + +.fa-dizzy::before { + content: "\f567"; } + +.fa-egg::before { + content: "\f7fb"; } + +.fa-house-medical-circle-xmark::before { + content: "\e513"; } + +.fa-campground::before { + content: "\f6bb"; } + +.fa-folder-plus::before { + content: "\f65e"; } + +.fa-futbol::before { + content: "\f1e3"; } + +.fa-futbol-ball::before { + content: "\f1e3"; } + +.fa-soccer-ball::before { + content: "\f1e3"; } + +.fa-paintbrush::before { + content: "\f1fc"; } + +.fa-paint-brush::before { + content: "\f1fc"; } + +.fa-lock::before { + content: "\f023"; } + +.fa-gas-pump::before { + content: "\f52f"; } + +.fa-hot-tub-person::before { + content: "\f593"; } + +.fa-hot-tub::before { + content: "\f593"; } + +.fa-map-location::before { + content: "\f59f"; } + +.fa-map-marked::before { + content: "\f59f"; } + +.fa-house-flood-water::before { + content: "\e50e"; } + +.fa-tree::before { + content: "\f1bb"; } + +.fa-bridge-lock::before { + content: "\e4cc"; } + +.fa-sack-dollar::before { + content: "\f81d"; } + +.fa-pen-to-square::before { + content: "\f044"; } + +.fa-edit::before { + content: "\f044"; } + +.fa-car-side::before { + content: "\f5e4"; } + +.fa-share-nodes::before { + content: "\f1e0"; } + +.fa-share-alt::before { + content: "\f1e0"; } + +.fa-heart-circle-minus::before { + content: "\e4ff"; } + +.fa-hourglass-half::before { + content: "\f252"; } + +.fa-hourglass-2::before { + content: "\f252"; } + +.fa-microscope::before { + content: "\f610"; } + +.fa-sink::before { + content: "\e06d"; } + +.fa-bag-shopping::before { + content: "\f290"; } + +.fa-shopping-bag::before { + content: "\f290"; } + +.fa-arrow-down-z-a::before { + content: "\f881"; } + +.fa-sort-alpha-desc::before { + content: "\f881"; } + +.fa-sort-alpha-down-alt::before { + content: "\f881"; } + +.fa-mitten::before { + content: "\f7b5"; } + +.fa-person-rays::before { + content: "\e54d"; } + +.fa-users::before { + content: "\f0c0"; } + +.fa-eye-slash::before { + content: "\f070"; } + +.fa-flask-vial::before { + content: "\e4f3"; } + 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} + +.fa-building::before { + content: "\f1ad"; } + +.fa-angles-left::before { + content: "\f100"; } + +.fa-angle-double-left::before { + content: "\f100"; } + +.fa-qrcode::before { + content: "\f029"; } + +.fa-clock-rotate-left::before { + content: "\f1da"; } + +.fa-history::before { + content: "\f1da"; } + +.fa-face-grin-beam-sweat::before { + content: "\f583"; } + +.fa-grin-beam-sweat::before { + content: "\f583"; } + +.fa-file-export::before { + content: "\f56e"; } + +.fa-arrow-right-from-file::before { + content: "\f56e"; } + +.fa-shield::before { + content: "\f132"; } + +.fa-shield-blank::before { + content: "\f132"; } + +.fa-arrow-up-short-wide::before { + content: "\f885"; } + +.fa-sort-amount-up-alt::before { + content: "\f885"; } + +.fa-house-medical::before { + content: "\e3b2"; } + +.fa-golf-ball-tee::before { + content: "\f450"; } + +.fa-golf-ball::before { + content: "\f450"; } + +.fa-circle-chevron-left::before { + content: "\f137"; } + +.fa-chevron-circle-left::before { + content: "\f137"; } + +.fa-house-chimney-window::before { + content: "\e00d"; } + +.fa-pen-nib::before { + content: "\f5ad"; } + +.fa-tent-arrow-turn-left::before { + content: "\e580"; } + +.fa-tents::before { + content: "\e582"; } + +.fa-wand-magic::before { + content: "\f0d0"; } + +.fa-magic::before { + content: "\f0d0"; } + +.fa-dog::before { + content: "\f6d3"; } + +.fa-carrot::before { + content: "\f787"; } + +.fa-moon::before { + content: "\f186"; } + +.fa-wine-glass-empty::before { + content: "\f5ce"; } + +.fa-wine-glass-alt::before { + content: "\f5ce"; } + +.fa-cheese::before { + content: "\f7ef"; } + +.fa-yin-yang::before { + content: "\f6ad"; } + +.fa-music::before { + content: "\f001"; } + +.fa-code-commit::before { + content: "\f386"; } + +.fa-temperature-low::before { + content: "\f76b"; } + +.fa-person-biking::before { + content: "\f84a"; } + +.fa-biking::before { + content: "\f84a"; } + +.fa-broom::before { + content: "\f51a"; } + +.fa-shield-heart::before { + content: "\e574"; } + +.fa-gopuram::before { + content: "\f664"; } + +.fa-earth-oceania::before { + content: "\e47b"; } + +.fa-globe-oceania::before { + content: "\e47b"; } + +.fa-square-xmark::before { + content: "\f2d3"; } + +.fa-times-square::before { + content: "\f2d3"; } + +.fa-xmark-square::before { + content: "\f2d3"; } + +.fa-hashtag::before { + content: "\23"; } + +.fa-up-right-and-down-left-from-center::before { + content: "\f424"; } + +.fa-expand-alt::before { + content: "\f424"; } + +.fa-oil-can::before { + content: "\f613"; } + +.fa-t::before { + content: "\54"; } + +.fa-hippo::before { + content: "\f6ed"; } + +.fa-chart-column::before { + content: "\e0e3"; } + +.fa-infinity::before { + content: "\f534"; } + +.fa-vial-circle-check::before { + content: "\e596"; } + +.fa-person-arrow-down-to-line::before { + content: "\e538"; } + +.fa-voicemail::before { + content: "\f897"; } + +.fa-fan::before { + content: "\f863"; } + +.fa-person-walking-luggage::before { + content: "\e554"; } + +.fa-up-down::before { + content: "\f338"; } + +.fa-arrows-alt-v::before { + content: "\f338"; } + +.fa-cloud-moon-rain::before { + content: "\f73c"; } + +.fa-calendar::before { + content: "\f133"; } + +.fa-trailer::before { + content: "\e041"; } + +.fa-bahai::before { + content: "\f666"; } + +.fa-haykal::before { + content: "\f666"; } + +.fa-sd-card::before { + content: "\f7c2"; } + +.fa-dragon::before { + content: "\f6d5"; } + +.fa-shoe-prints::before { + content: "\f54b"; } + +.fa-circle-plus::before { + content: "\f055"; } + +.fa-plus-circle::before { + content: "\f055"; } + +.fa-face-grin-tongue-wink::before { + content: "\f58b"; } + +.fa-grin-tongue-wink::before { + content: "\f58b"; } + +.fa-hand-holding::before { + content: "\f4bd"; } + +.fa-plug-circle-exclamation::before { + content: "\e55d"; } + +.fa-link-slash::before { + content: "\f127"; } + +.fa-chain-broken::before { + content: "\f127"; } + +.fa-chain-slash::before { + content: "\f127"; } + 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"\f104"; } + +.fa-diagram-successor::before { + content: "\e47a"; } + +.fa-truck-arrow-right::before { + content: "\e58b"; } + +.fa-arrows-split-up-and-left::before { + content: "\e4bc"; } + +.fa-hand-fist::before { + content: "\f6de"; } + +.fa-fist-raised::before { + content: "\f6de"; } + +.fa-cloud-moon::before { + content: "\f6c3"; } + +.fa-briefcase::before { + content: "\f0b1"; } + +.fa-person-falling::before { + content: "\e546"; } + +.fa-image-portrait::before { + content: "\f3e0"; } + +.fa-portrait::before { + content: "\f3e0"; } + +.fa-user-tag::before { + content: "\f507"; } + +.fa-rug::before { + content: "\e569"; } + +.fa-earth-europe::before { + content: "\f7a2"; } + +.fa-globe-europe::before { + content: "\f7a2"; } + +.fa-cart-flatbed-suitcase::before { + content: "\f59d"; } + +.fa-luggage-cart::before { + content: "\f59d"; } + +.fa-rectangle-xmark::before { + content: "\f410"; } + +.fa-rectangle-times::before { + content: "\f410"; } + +.fa-times-rectangle::before { + content: "\f410"; } + +.fa-window-close::before { + content: "\f410"; } + +.fa-baht-sign::before { + content: "\e0ac"; } + +.fa-book-open::before { + content: "\f518"; } + +.fa-book-journal-whills::before { + content: "\f66a"; } + +.fa-journal-whills::before { + content: "\f66a"; } + +.fa-handcuffs::before { + content: "\e4f8"; } + +.fa-triangle-exclamation::before { + content: "\f071"; } + +.fa-exclamation-triangle::before { + content: "\f071"; } + +.fa-warning::before { + content: "\f071"; } + +.fa-database::before { + content: "\f1c0"; } + +.fa-share::before { + content: "\f064"; } + +.fa-arrow-turn-right::before { + content: "\f064"; } + +.fa-mail-forward::before { + content: "\f064"; } + +.fa-bottle-droplet::before { + content: "\e4c4"; } + +.fa-mask-face::before { + content: "\e1d7"; } + +.fa-hill-rockslide::before { + content: "\e508"; } + +.fa-right-left::before { + content: "\f362"; } + +.fa-exchange-alt::before { + content: "\f362"; } + +.fa-paper-plane::before { + content: "\f1d8"; } + +.fa-road-circle-exclamation::before { + content: "\e565"; } + +.fa-dungeon::before { + content: "\f6d9"; } + +.fa-align-right::before { + content: "\f038"; } + +.fa-money-bill-1-wave::before { + content: "\f53b"; } + +.fa-money-bill-wave-alt::before { + content: "\f53b"; } + +.fa-life-ring::before { + content: "\f1cd"; } + +.fa-hands::before { + content: "\f2a7"; } + +.fa-sign-language::before { + content: "\f2a7"; } + +.fa-signing::before { + content: "\f2a7"; } + +.fa-calendar-day::before { + content: "\f783"; } + +.fa-water-ladder::before { + content: "\f5c5"; } + +.fa-ladder-water::before { + content: "\f5c5"; } + +.fa-swimming-pool::before { + content: "\f5c5"; } + +.fa-arrows-up-down::before { + content: "\f07d"; } + +.fa-arrows-v::before { + content: "\f07d"; } + +.fa-face-grimace::before { + content: "\f57f"; } + +.fa-grimace::before { + content: "\f57f"; } + +.fa-wheelchair-move::before { + content: "\e2ce"; } + +.fa-wheelchair-alt::before { + content: "\e2ce"; } + +.fa-turn-down::before { + content: "\f3be"; } + +.fa-level-down-alt::before { + content: "\f3be"; } + +.fa-person-walking-arrow-right::before { + content: "\e552"; } + +.fa-square-envelope::before { + content: "\f199"; } + +.fa-envelope-square::before { + content: "\f199"; } + +.fa-dice::before { + content: "\f522"; } + +.fa-bowling-ball::before { + content: "\f436"; } + +.fa-brain::before { + content: "\f5dc"; } + +.fa-bandage::before { + content: "\f462"; } + +.fa-band-aid::before { + content: "\f462"; } + +.fa-calendar-minus::before { + content: "\f272"; } + +.fa-circle-xmark::before { + content: "\f057"; } + +.fa-times-circle::before { + content: "\f057"; } + +.fa-xmark-circle::before { + content: "\f057"; } + +.fa-gifts::before { + content: "\f79c"; } + +.fa-hotel::before { + content: "\f594"; } + +.fa-earth-asia::before { + content: "\f57e"; } + +.fa-globe-asia::before { + content: "\f57e"; } + +.fa-id-card-clip::before { + content: "\f47f"; } + +.fa-id-card-alt::before { + content: "\f47f"; } + +.fa-magnifying-glass-plus::before { + content: "\f00e"; } + +.fa-search-plus::before { + content: "\f00e"; } + +.fa-thumbs-up::before { + content: "\f164"; } + +.fa-user-clock::before { + content: "\f4fd"; } + +.fa-hand-dots::before { + content: "\f461"; } + +.fa-allergies::before { + content: "\f461"; } + +.fa-file-invoice::before { + content: "\f570"; } + +.fa-window-minimize::before { + content: "\f2d1"; } + +.fa-mug-saucer::before { + content: "\f0f4"; } + +.fa-coffee::before { + content: "\f0f4"; } + +.fa-brush::before { + content: "\f55d"; } + +.fa-mask::before { + content: "\f6fa"; } + +.fa-magnifying-glass-minus::before { + content: "\f010"; } + +.fa-search-minus::before { + content: "\f010"; } + +.fa-ruler-vertical::before { + content: "\f548"; } + +.fa-user-large::before { + content: "\f406"; } + +.fa-user-alt::before { + content: "\f406"; } + +.fa-train-tram::before { + content: "\e5b4"; } + +.fa-user-nurse::before { + content: "\f82f"; } + +.fa-syringe::before { + content: "\f48e"; } + +.fa-cloud-sun::before { + content: "\f6c4"; } + +.fa-stopwatch-20::before { + content: "\e06f"; } + +.fa-square-full::before { + content: "\f45c"; } + +.fa-magnet::before { + content: "\f076"; } + +.fa-jar::before { + content: "\e516"; } + +.fa-note-sticky::before { + content: "\f249"; } + +.fa-sticky-note::before { + content: "\f249"; } + +.fa-bug-slash::before { + content: "\e490"; } + +.fa-arrow-up-from-water-pump::before { + content: "\e4b6"; } + +.fa-bone::before { + content: "\f5d7"; } + +.fa-user-injured::before { + content: "\f728"; } + +.fa-face-sad-tear::before { + content: "\f5b4"; } + +.fa-sad-tear::before { + content: "\f5b4"; } + +.fa-plane::before { + content: "\f072"; } + +.fa-tent-arrows-down::before { + content: "\e581"; } + +.fa-exclamation::before { + content: "\21"; } + +.fa-arrows-spin::before { + content: "\e4bb"; } + +.fa-print::before { + content: "\f02f"; } + +.fa-turkish-lira-sign::before { + content: "\e2bb"; } + +.fa-try::before { + content: "\e2bb"; } + +.fa-turkish-lira::before { + content: "\e2bb"; } + +.fa-dollar-sign::before { + content: "\24"; } + +.fa-dollar::before { + content: "\24"; } + +.fa-usd::before { + content: "\24"; } + +.fa-x::before { + content: "\58"; } + +.fa-magnifying-glass-dollar::before { + content: "\f688"; } + +.fa-search-dollar::before { + content: "\f688"; } + +.fa-users-gear::before { + content: "\f509"; } + +.fa-users-cog::before { + content: "\f509"; } + +.fa-person-military-pointing::before { + content: "\e54a"; } + +.fa-building-columns::before { + content: "\f19c"; } + +.fa-bank::before { + content: "\f19c"; } + +.fa-institution::before { + content: "\f19c"; } + +.fa-museum::before { + content: "\f19c"; } + +.fa-university::before { + content: "\f19c"; } + +.fa-umbrella::before { + content: "\f0e9"; } + +.fa-trowel::before { + content: "\e589"; } + +.fa-d::before { + content: "\44"; } + +.fa-stapler::before { + content: "\e5af"; } + +.fa-masks-theater::before { + content: "\f630"; } + +.fa-theater-masks::before { + content: "\f630"; } + +.fa-kip-sign::before { + content: "\e1c4"; } + +.fa-hand-point-left::before { + content: "\f0a5"; } + +.fa-handshake-simple::before { + content: "\f4c6"; } + +.fa-handshake-alt::before { + content: "\f4c6"; } + +.fa-jet-fighter::before { + content: "\f0fb"; } + +.fa-fighter-jet::before { + content: "\f0fb"; } + +.fa-square-share-nodes::before { + content: "\f1e1"; } + +.fa-share-alt-square::before { + content: "\f1e1"; } + +.fa-barcode::before { + content: "\f02a"; } + +.fa-plus-minus::before { + content: "\e43c"; } + +.fa-video::before { + content: "\f03d"; } + +.fa-video-camera::before { + content: "\f03d"; } + +.fa-graduation-cap::before { + content: "\f19d"; } + +.fa-mortar-board::before { + content: "\f19d"; } + +.fa-hand-holding-medical::before { + content: "\e05c"; } + +.fa-person-circle-check::before { + content: "\e53e"; } + +.fa-turn-up::before { + content: "\f3bf"; } + +.fa-level-up-alt::before { + content: "\f3bf"; } + +.sr-only, +.fa-sr-only { + position: absolute; + width: 1px; + height: 1px; + padding: 0; + margin: -1px; + overflow: hidden; + clip: rect(0, 0, 0, 0); + white-space: nowrap; + border-width: 0; } + +.sr-only-focusable:not(:focus), +.fa-sr-only-focusable:not(:focus) { + position: absolute; + width: 1px; + height: 1px; + padding: 0; + margin: -1px; + overflow: hidden; + clip: rect(0, 0, 0, 0); + white-space: nowrap; + border-width: 0; } +:root, :host { + --fa-style-family-brands: 'Font Awesome 6 Brands'; + --fa-font-brands: normal 400 1em/1 'Font Awesome 6 Brands'; } + +@font-face { + font-family: 'Font Awesome 6 Brands'; + font-style: normal; + font-weight: 400; + font-display: block; + src: url("../webfonts/fa-brands-400.woff2") format("woff2"), url("../webfonts/fa-brands-400.ttf") format("truetype"); } + +.fab, +.fa-brands { + font-weight: 400; } + +.fa-monero:before { + content: "\f3d0"; } + +.fa-hooli:before { + content: "\f427"; } + +.fa-yelp:before { + content: "\f1e9"; } + +.fa-cc-visa:before { + content: "\f1f0"; } + +.fa-lastfm:before { + content: "\f202"; } + +.fa-shopware:before { + content: "\f5b5"; } + +.fa-creative-commons-nc:before { + content: "\f4e8"; } + +.fa-aws:before { + content: "\f375"; } + +.fa-redhat:before { + content: "\f7bc"; } + +.fa-yoast:before { + content: "\f2b1"; } + +.fa-cloudflare:before { + content: "\e07d"; } + +.fa-ups:before { + content: "\f7e0"; } + +.fa-wpexplorer:before { + content: "\f2de"; } + +.fa-dyalog:before { + content: "\f399"; } + +.fa-bity:before { + content: "\f37a"; } + +.fa-stackpath:before { + content: "\f842"; } + +.fa-buysellads:before { + content: "\f20d"; } + +.fa-first-order:before { + content: "\f2b0"; } + +.fa-modx:before { + content: "\f285"; } + +.fa-guilded:before { + content: "\e07e"; } + +.fa-vnv:before { + content: "\f40b"; } + +.fa-square-js:before { + content: "\f3b9"; } + +.fa-js-square:before { + content: "\f3b9"; } + +.fa-microsoft:before { + content: "\f3ca"; } + +.fa-qq:before { + content: "\f1d6"; } + +.fa-orcid:before { + content: "\f8d2"; } + +.fa-java:before { + content: "\f4e4"; } + +.fa-invision:before { + content: "\f7b0"; } + +.fa-creative-commons-pd-alt:before { + content: "\f4ed"; } + +.fa-centercode:before { + content: "\f380"; } + +.fa-glide-g:before { + content: "\f2a6"; } + +.fa-drupal:before { + content: "\f1a9"; } + +.fa-hire-a-helper:before { + content: "\f3b0"; } + +.fa-creative-commons-by:before { + content: "\f4e7"; } + +.fa-unity:before { + content: "\e049"; } + +.fa-whmcs:before { + content: "\f40d"; } + +.fa-rocketchat:before { + content: "\f3e8"; } + +.fa-vk:before { + content: "\f189"; } + +.fa-untappd:before { + content: "\f405"; } + +.fa-mailchimp:before { + content: "\f59e"; } + +.fa-css3-alt:before { + content: "\f38b"; } + +.fa-square-reddit:before { + content: "\f1a2"; } + +.fa-reddit-square:before { + content: "\f1a2"; } + +.fa-vimeo-v:before { + content: "\f27d"; } + +.fa-contao:before { + content: "\f26d"; } + +.fa-square-font-awesome:before { + content: "\e5ad"; } + +.fa-deskpro:before { + content: "\f38f"; } + +.fa-sistrix:before { + content: "\f3ee"; } + +.fa-square-instagram:before { + content: "\e055"; } + +.fa-instagram-square:before { + content: "\e055"; } + +.fa-battle-net:before { + content: "\f835"; } + +.fa-the-red-yeti:before { + content: "\f69d"; } + +.fa-square-hacker-news:before { + content: "\f3af"; } + +.fa-hacker-news-square:before { + content: "\f3af"; } + +.fa-edge:before { + content: "\f282"; } + +.fa-threads:before { + content: "\e618"; } + +.fa-napster:before { + content: "\f3d2"; } + +.fa-square-snapchat:before { + content: "\f2ad"; } + +.fa-snapchat-square:before { + content: "\f2ad"; } + +.fa-google-plus-g:before { + content: "\f0d5"; } + +.fa-artstation:before { + content: "\f77a"; } + +.fa-markdown:before { + content: "\f60f"; } + +.fa-sourcetree:before { + content: "\f7d3"; } + +.fa-google-plus:before { + content: "\f2b3"; } + 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+.fa.fa-linkedin-square:before { + content: "\f08c"; } + +.fa.fa-thumb-tack:before { + content: "\f08d"; } + +.fa.fa-external-link:before { + content: "\f35d"; } + +.fa.fa-sign-in:before { + content: "\f2f6"; } + +.fa.fa-github-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-github-square:before { + content: "\f092"; } + +.fa.fa-lemon-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-lemon-o:before { + content: "\f094"; } + +.fa.fa-square-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-square-o:before { + content: "\f0c8"; } + +.fa.fa-bookmark-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-bookmark-o:before { + content: "\f02e"; } + +.fa.fa-twitter { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-facebook { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-facebook:before { + content: "\f39e"; } + +.fa.fa-facebook-f { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-facebook-f:before { + content: "\f39e"; } + +.fa.fa-github { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-credit-card { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-feed:before { + content: "\f09e"; } + +.fa.fa-hdd-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hdd-o:before { + content: "\f0a0"; } + +.fa.fa-hand-o-right { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-o-right:before { + content: "\f0a4"; } + +.fa.fa-hand-o-left { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-o-left:before { + content: "\f0a5"; } + +.fa.fa-hand-o-up { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-o-up:before { + content: "\f0a6"; } + +.fa.fa-hand-o-down { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-o-down:before { + content: "\f0a7"; } + +.fa.fa-globe:before { + content: "\f57d"; } + +.fa.fa-tasks:before { + content: "\f828"; } + +.fa.fa-arrows-alt:before { + content: "\f31e"; } + +.fa.fa-group:before { + content: "\f0c0"; } + +.fa.fa-chain:before { + content: "\f0c1"; } + +.fa.fa-cut:before { + content: "\f0c4"; } + +.fa.fa-files-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-files-o:before { + content: "\f0c5"; } + +.fa.fa-floppy-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-floppy-o:before { + content: "\f0c7"; } + +.fa.fa-save { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-save:before { + content: "\f0c7"; } + +.fa.fa-navicon:before { + content: "\f0c9"; } + +.fa.fa-reorder:before { + content: "\f0c9"; } + +.fa.fa-magic:before { + content: "\e2ca"; } + +.fa.fa-pinterest { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pinterest-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pinterest-square:before { + content: "\f0d3"; } + +.fa.fa-google-plus-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-google-plus-square:before { + content: "\f0d4"; } + +.fa.fa-google-plus { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-google-plus:before { + content: "\f0d5"; } + +.fa.fa-money:before { + content: "\f3d1"; } + +.fa.fa-unsorted:before { + content: "\f0dc"; } + +.fa.fa-sort-desc:before { + content: "\f0dd"; } + +.fa.fa-sort-asc:before { + content: "\f0de"; } + +.fa.fa-linkedin { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-linkedin:before { + content: "\f0e1"; } + +.fa.fa-rotate-left:before { + content: "\f0e2"; } + +.fa.fa-legal:before { + content: "\f0e3"; } + +.fa.fa-tachometer:before { + content: "\f625"; } + +.fa.fa-dashboard:before { + content: "\f625"; } + +.fa.fa-comment-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-comment-o:before { + content: "\f075"; } + +.fa.fa-comments-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-comments-o:before { + content: "\f086"; } + +.fa.fa-flash:before { + content: "\f0e7"; } + +.fa.fa-clipboard:before { + content: "\f0ea"; } + +.fa.fa-lightbulb-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-lightbulb-o:before { + content: "\f0eb"; } + +.fa.fa-exchange:before { + content: "\f362"; } + +.fa.fa-cloud-download:before { + content: "\f0ed"; } + +.fa.fa-cloud-upload:before { + content: "\f0ee"; } + +.fa.fa-bell-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-bell-o:before { + content: "\f0f3"; } + +.fa.fa-cutlery:before { + content: "\f2e7"; } + +.fa.fa-file-text-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-text-o:before { + content: "\f15c"; } + +.fa.fa-building-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-building-o:before { + content: "\f1ad"; } + +.fa.fa-hospital-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hospital-o:before { + content: "\f0f8"; } + +.fa.fa-tablet:before { + content: "\f3fa"; } + +.fa.fa-mobile:before { + content: "\f3cd"; } + +.fa.fa-mobile-phone:before { + content: "\f3cd"; } + +.fa.fa-circle-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-circle-o:before { + content: "\f111"; } + +.fa.fa-mail-reply:before { + content: "\f3e5"; } + +.fa.fa-github-alt { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-folder-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-folder-o:before { + content: "\f07b"; } + +.fa.fa-folder-open-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-folder-open-o:before { + content: "\f07c"; } + +.fa.fa-smile-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-smile-o:before { + content: "\f118"; } + +.fa.fa-frown-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-frown-o:before { + content: "\f119"; } + +.fa.fa-meh-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-meh-o:before { + content: "\f11a"; } + +.fa.fa-keyboard-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-keyboard-o:before { + content: "\f11c"; } + +.fa.fa-flag-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-flag-o:before { + content: "\f024"; } + +.fa.fa-mail-reply-all:before { + content: "\f122"; } + +.fa.fa-star-half-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-star-half-o:before { + content: "\f5c0"; } + +.fa.fa-star-half-empty { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-star-half-empty:before { + content: "\f5c0"; } + +.fa.fa-star-half-full { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-star-half-full:before { + content: "\f5c0"; } + +.fa.fa-code-fork:before { + content: "\f126"; } + +.fa.fa-chain-broken:before { + content: "\f127"; } + +.fa.fa-unlink:before { + content: "\f127"; } + +.fa.fa-calendar-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-calendar-o:before { + content: "\f133"; } + +.fa.fa-maxcdn { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-html5 { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-css3 { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-unlock-alt:before { + content: "\f09c"; } + +.fa.fa-minus-square-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-minus-square-o:before { + content: "\f146"; } + +.fa.fa-level-up:before { + content: "\f3bf"; } + +.fa.fa-level-down:before { + content: "\f3be"; } + +.fa.fa-pencil-square:before { + content: "\f14b"; } + +.fa.fa-external-link-square:before { + content: "\f360"; } + +.fa.fa-compass { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-down { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-down:before { + content: "\f150"; } + +.fa.fa-toggle-down { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-toggle-down:before { + content: "\f150"; } + +.fa.fa-caret-square-o-up { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-up:before { + content: "\f151"; } + +.fa.fa-toggle-up { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-toggle-up:before { + content: "\f151"; } + +.fa.fa-caret-square-o-right { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-right:before { + content: "\f152"; } + +.fa.fa-toggle-right { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-toggle-right:before { + content: "\f152"; } + +.fa.fa-eur:before { + content: "\f153"; } + +.fa.fa-euro:before { + content: "\f153"; } + +.fa.fa-gbp:before { + content: "\f154"; } + +.fa.fa-usd:before { + content: "\24"; } + +.fa.fa-dollar:before { + content: "\24"; } + +.fa.fa-inr:before { + content: "\e1bc"; } + +.fa.fa-rupee:before { + content: "\e1bc"; } + +.fa.fa-jpy:before { + content: "\f157"; } + +.fa.fa-cny:before { + content: "\f157"; } + +.fa.fa-rmb:before { + content: "\f157"; } + +.fa.fa-yen:before { + content: "\f157"; } + +.fa.fa-rub:before { + content: "\f158"; } + +.fa.fa-ruble:before { + content: "\f158"; } + +.fa.fa-rouble:before { + content: "\f158"; } + +.fa.fa-krw:before { + content: "\f159"; } + +.fa.fa-won:before { + content: "\f159"; } + +.fa.fa-btc { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitcoin { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitcoin:before { + content: "\f15a"; } + +.fa.fa-file-text:before { + content: "\f15c"; } + +.fa.fa-sort-alpha-asc:before { + content: "\f15d"; } + +.fa.fa-sort-alpha-desc:before { + content: "\f881"; } + +.fa.fa-sort-amount-asc:before { + content: "\f884"; } + +.fa.fa-sort-amount-desc:before { + content: "\f160"; } + +.fa.fa-sort-numeric-asc:before { + content: "\f162"; } + +.fa.fa-sort-numeric-desc:before { + content: "\f886"; } + +.fa.fa-youtube-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-youtube-square:before { + content: "\f431"; } + +.fa.fa-youtube { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-xing { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-xing-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-xing-square:before { + content: "\f169"; } + +.fa.fa-youtube-play { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-youtube-play:before { + content: "\f167"; } + +.fa.fa-dropbox { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-stack-overflow { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-instagram { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-flickr { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-adn { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitbucket { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitbucket-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitbucket-square:before { + content: "\f171"; } + +.fa.fa-tumblr { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-tumblr-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-tumblr-square:before { + content: "\f174"; } + +.fa.fa-long-arrow-down:before { + content: "\f309"; } + +.fa.fa-long-arrow-up:before { + content: "\f30c"; } + +.fa.fa-long-arrow-left:before { + content: "\f30a"; } + +.fa.fa-long-arrow-right:before { + content: "\f30b"; } + +.fa.fa-apple { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-windows { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-android { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-linux { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-dribbble { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-skype { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-foursquare { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-trello { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gratipay { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gittip { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gittip:before { + content: "\f184"; } + +.fa.fa-sun-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-sun-o:before { + content: "\f185"; } + +.fa.fa-moon-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-moon-o:before { + content: "\f186"; } + +.fa.fa-vk { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-weibo { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-renren { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pagelines { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-stack-exchange { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-arrow-circle-o-right { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-arrow-circle-o-right:before { + content: "\f35a"; } + +.fa.fa-arrow-circle-o-left { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-arrow-circle-o-left:before { + content: "\f359"; } + +.fa.fa-caret-square-o-left { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-left:before { + content: "\f191"; } + +.fa.fa-toggle-left { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-toggle-left:before { + content: "\f191"; } + +.fa.fa-dot-circle-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-dot-circle-o:before { + content: "\f192"; } + +.fa.fa-vimeo-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-vimeo-square:before { + content: "\f194"; } + +.fa.fa-try:before { + content: "\e2bb"; } + +.fa.fa-turkish-lira:before { + content: "\e2bb"; } + +.fa.fa-plus-square-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-plus-square-o:before { + content: "\f0fe"; } + +.fa.fa-slack { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wordpress { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-openid { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-institution:before { + content: "\f19c"; } + +.fa.fa-bank:before { + content: "\f19c"; } + +.fa.fa-mortar-board:before { + content: "\f19d"; } + +.fa.fa-yahoo { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-google { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-reddit { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-reddit-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-reddit-square:before { + content: "\f1a2"; } + +.fa.fa-stumbleupon-circle { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-stumbleupon { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-delicious { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-digg { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pied-piper-pp { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pied-piper-alt { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-drupal { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-joomla { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-behance { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-behance-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-behance-square:before { + content: "\f1b5"; } + +.fa.fa-steam { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-steam-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-steam-square:before { + content: "\f1b7"; } + +.fa.fa-automobile:before { + content: "\f1b9"; } + +.fa.fa-cab:before { + content: "\f1ba"; } + +.fa.fa-spotify { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-deviantart { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-soundcloud { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-file-pdf-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-pdf-o:before { + content: "\f1c1"; } + +.fa.fa-file-word-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-word-o:before { + content: "\f1c2"; } + +.fa.fa-file-excel-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-excel-o:before { + content: "\f1c3"; } + +.fa.fa-file-powerpoint-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-powerpoint-o:before { + content: "\f1c4"; } + +.fa.fa-file-image-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-image-o:before { + content: "\f1c5"; } + +.fa.fa-file-photo-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-photo-o:before { + content: "\f1c5"; } + +.fa.fa-file-picture-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-picture-o:before { + content: "\f1c5"; } + +.fa.fa-file-archive-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-archive-o:before { + content: "\f1c6"; } + +.fa.fa-file-zip-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-zip-o:before { + content: "\f1c6"; } + +.fa.fa-file-audio-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-audio-o:before { + content: "\f1c7"; } + +.fa.fa-file-sound-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-sound-o:before { + content: "\f1c7"; } + +.fa.fa-file-video-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-video-o:before { + content: "\f1c8"; } + +.fa.fa-file-movie-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-movie-o:before { + content: "\f1c8"; } + +.fa.fa-file-code-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-code-o:before { + content: "\f1c9"; } + +.fa.fa-vine { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-codepen { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-jsfiddle { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-life-bouy:before { + content: "\f1cd"; } + +.fa.fa-life-buoy:before { + content: "\f1cd"; } + +.fa.fa-life-saver:before { + content: "\f1cd"; } + +.fa.fa-support:before { + content: "\f1cd"; } + +.fa.fa-circle-o-notch:before { + content: "\f1ce"; } + +.fa.fa-rebel { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-ra { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-ra:before { + content: "\f1d0"; } + +.fa.fa-resistance { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-resistance:before { + content: "\f1d0"; } + +.fa.fa-empire { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-ge { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-ge:before { + content: "\f1d1"; } + +.fa.fa-git-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-git-square:before { + content: "\f1d2"; } + +.fa.fa-git { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-hacker-news { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-y-combinator-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-y-combinator-square:before { + content: "\f1d4"; } + +.fa.fa-yc-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yc-square:before { + content: "\f1d4"; } + +.fa.fa-tencent-weibo { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-qq { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-weixin { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wechat { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wechat:before { + content: "\f1d7"; } + +.fa.fa-send:before { + content: "\f1d8"; } + +.fa.fa-paper-plane-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-paper-plane-o:before { + content: "\f1d8"; } + +.fa.fa-send-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-send-o:before { + content: "\f1d8"; } + +.fa.fa-circle-thin { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-circle-thin:before { + content: "\f111"; } + +.fa.fa-header:before { + content: "\f1dc"; } + +.fa.fa-futbol-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-futbol-o:before { + content: "\f1e3"; } + +.fa.fa-soccer-ball-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-soccer-ball-o:before { + content: "\f1e3"; } + +.fa.fa-slideshare { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-twitch { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yelp { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-newspaper-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-newspaper-o:before { + content: "\f1ea"; } + +.fa.fa-paypal { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-google-wallet { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-visa { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-mastercard { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-discover { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-amex { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-paypal { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-stripe { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bell-slash-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-bell-slash-o:before { + content: "\f1f6"; } + +.fa.fa-trash:before { + content: "\f2ed"; } + +.fa.fa-copyright { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-eyedropper:before { + content: "\f1fb"; } + +.fa.fa-area-chart:before { + content: "\f1fe"; } + +.fa.fa-pie-chart:before { + content: "\f200"; } + +.fa.fa-line-chart:before { + content: "\f201"; } + +.fa.fa-lastfm { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-lastfm-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-lastfm-square:before { + content: "\f203"; } + +.fa.fa-ioxhost { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-angellist { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-cc:before { + content: "\f20a"; } + +.fa.fa-ils:before { + content: "\f20b"; } + +.fa.fa-shekel:before { + content: "\f20b"; } + +.fa.fa-sheqel:before { + content: "\f20b"; } + +.fa.fa-buysellads { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-connectdevelop { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-dashcube { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-forumbee { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-leanpub { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-sellsy { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-shirtsinbulk { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-simplybuilt { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-skyatlas { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-diamond { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-diamond:before { + content: "\f3a5"; } + +.fa.fa-transgender:before { + content: "\f224"; } + +.fa.fa-intersex:before { + content: "\f224"; } + +.fa.fa-transgender-alt:before { + content: "\f225"; } + +.fa.fa-facebook-official { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-facebook-official:before { + content: "\f09a"; } + +.fa.fa-pinterest-p { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-whatsapp { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-hotel:before { + content: "\f236"; } + +.fa.fa-viacoin { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-medium { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-y-combinator { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yc { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yc:before { + content: "\f23b"; } + +.fa.fa-optin-monster { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-opencart { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-expeditedssl { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-battery-4:before { + content: "\f240"; } + +.fa.fa-battery:before { + content: "\f240"; } + +.fa.fa-battery-3:before { + content: "\f241"; } + +.fa.fa-battery-2:before { + content: "\f242"; } + +.fa.fa-battery-1:before { + content: "\f243"; } + +.fa.fa-battery-0:before { + content: "\f244"; } + +.fa.fa-object-group { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-object-ungroup { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-sticky-note-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-sticky-note-o:before { + content: "\f249"; } + +.fa.fa-cc-jcb { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-diners-club { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-clone { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hourglass-o:before { + content: "\f254"; } + +.fa.fa-hourglass-1:before { + content: "\f251"; } + +.fa.fa-hourglass-2:before { + content: "\f252"; } + +.fa.fa-hourglass-3:before { + content: "\f253"; } + +.fa.fa-hand-rock-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-rock-o:before { + content: "\f255"; } + +.fa.fa-hand-grab-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-grab-o:before { + content: "\f255"; } + +.fa.fa-hand-paper-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-paper-o:before { + content: "\f256"; } + +.fa.fa-hand-stop-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-stop-o:before { + content: "\f256"; } + +.fa.fa-hand-scissors-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-scissors-o:before { + content: "\f257"; } + +.fa.fa-hand-lizard-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-lizard-o:before { + content: "\f258"; } + +.fa.fa-hand-spock-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-spock-o:before { + content: "\f259"; } + +.fa.fa-hand-pointer-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-pointer-o:before { + content: "\f25a"; } + +.fa.fa-hand-peace-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-peace-o:before { + content: "\f25b"; } + +.fa.fa-registered { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-creative-commons { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gg { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gg-circle { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-odnoklassniki { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-odnoklassniki-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-odnoklassniki-square:before { + content: "\f264"; } + +.fa.fa-get-pocket { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wikipedia-w { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-safari { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-chrome { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-firefox { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-opera { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-internet-explorer { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-television:before { + content: "\f26c"; } + +.fa.fa-contao { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-500px { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-amazon { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-calendar-plus-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-calendar-plus-o:before { + content: "\f271"; } + +.fa.fa-calendar-minus-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-calendar-minus-o:before { + content: "\f272"; } + +.fa.fa-calendar-times-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-calendar-times-o:before { + content: "\f273"; } + +.fa.fa-calendar-check-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-calendar-check-o:before { + content: "\f274"; } + +.fa.fa-map-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-map-o:before { + content: "\f279"; } + +.fa.fa-commenting:before { + content: "\f4ad"; } + +.fa.fa-commenting-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-commenting-o:before { + content: "\f4ad"; } + +.fa.fa-houzz { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-vimeo { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-vimeo:before { + content: "\f27d"; } + +.fa.fa-black-tie { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-fonticons { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-reddit-alien { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-edge { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-credit-card-alt:before { + content: "\f09d"; } + +.fa.fa-codiepie { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-modx { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-fort-awesome { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-usb { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-product-hunt { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-mixcloud { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-scribd { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pause-circle-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-pause-circle-o:before { + content: "\f28b"; } + +.fa.fa-stop-circle-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-stop-circle-o:before { + content: "\f28d"; } + +.fa.fa-bluetooth { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bluetooth-b { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gitlab { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wpbeginner { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wpforms { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-envira { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wheelchair-alt { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wheelchair-alt:before { + content: "\f368"; } + +.fa.fa-question-circle-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-question-circle-o:before { + content: "\f059"; } + +.fa.fa-volume-control-phone:before { + content: "\f2a0"; } + +.fa.fa-asl-interpreting:before { + content: "\f2a3"; } + +.fa.fa-deafness:before { + content: "\f2a4"; } + +.fa.fa-hard-of-hearing:before { + content: "\f2a4"; } + +.fa.fa-glide { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-glide-g { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-signing:before { + content: "\f2a7"; } + +.fa.fa-viadeo { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-viadeo-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-viadeo-square:before { + content: "\f2aa"; } + +.fa.fa-snapchat { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-snapchat-ghost { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-snapchat-ghost:before { + content: "\f2ab"; } + +.fa.fa-snapchat-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-snapchat-square:before { + content: "\f2ad"; } + +.fa.fa-pied-piper { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-first-order { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yoast { + 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+
+ + + + +
+ +
+
+
+

iUSEiSEE - The iSEE cookbook

+

Federico +Marini1, +Najla Abassi2

+
+Collection of material developed by the iSEE core dev team
(Kevin +Rue-Albrecht, Federico Marini, Charlotte Soneson, Aaron Lun)

+ + + Source: vignettes/iSEE_quickrecipes_03.Rmd + + +
+ + + +

+
+

Introduction +

+

This vignette consists of a series of (independent) hands-on recipes, +aimed at exploring the capabilities of iSEE (Rue-Albrecht et al. 2018) both interactively +and programmatically. Each recipe consists of a short task and a screen +shot of the desired appearance of the app instance that should be +created. For each recipe, we provide a set of hints, as well as detailed +instructions on how to solve the task both interactively (by clicking in +the app) and programmatically (by directly setting up and launching the +desired app instance).

+

For a general overview of the default iSEE panels, we +refer to the overview vignette. For +all the details about the panel classes and the associated slots, we +refer to the help pages for the respective panel class (e.g., +?ReducedDimensionPlot).

+
+
+

Prepare the session +

+

Before starting with the recipes, we need to load the required +packages and the demo data set.

+
+library("iSEE")
+library("iSEEu")
+
+sce_location <- system.file("datasets", "sce_pbmc3k.RDS", package = "iUSEiSEE")
+sce_location
+#> [1] "/Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/library/iUSEiSEE/datasets/sce_pbmc3k.RDS"
+sce <- readRDS(sce_location)
+sce
+#> class: SingleCellExperiment 
+#> dim: 32738 2643 
+#> metadata(0):
+#> assays(2): counts logcounts
+#> rownames(32738): MIR1302-10 FAM138A ... AC002321.2 AC002321.1
+#> rowData names(19): ENSEMBL_ID Symbol_TENx ... FDR_cluster11
+#>   FDR_cluster12
+#> colnames(2643): Cell1 Cell2 ... Cell2699 Cell2700
+#> colData names(24): Sample Barcode ... labels_ont cell_ontology_labels
+#> reducedDimNames(3): PCA TSNE UMAP
+#> mainExpName: NULL
+#> altExpNames(0):
+
+
+

The cookbook +

+

What follows is a list of 11 small self contained recipes, that try +to address one specific aim - and provide accordingly a simple, targeted +solution.

+
+

Recipe 1: Panel organisation +

+

Using the pbmc3k data set, create an app that contains +only a reduced dimension plot panel, a row data plot panel and a column +data plot panel. The widths of the three panels should be 3, 4 and 5 +units, respectively.

+
+Hints +
    +
  • +Panels can be added, removed and resized via the +Organization button in the top right corner of the app. +
    • +
  • +To pre-specify the panels to be included in the app, use the +initial argument to iSEE(). +
    • +
  • +You can remind yourself of the list of slot names available for each +panel class and their respective value using the str() +function on any instance of a panel object, +e.g. str(ReducedDimensionPlot()). +
    • +
  • +For more information about the different types of panels, see the overview vignette or the help pages for +the respective panel classes. +
    • +
+Solution (interactively) +
    +
  • +First open an application with the default set of panels. +
    • +
    +app <- iSEE(sce)
+shiny::runApp(app)
    +
  • +Click on the Organization button in the top right corner of +the app, and then click on Organize panels. +
    • +
  • +In the pop-up window that appears, click on the little x +next to all the panels that you want to remove (all but the +Reduced dimension plot 1, Column data plot 1 +and Row data plot 1). +
    • +
  • +Drag and drop the remaining three panel names in the correct order. +
    • +
  • +For each panel, set the correct panel width by modifying the value in +the Width dropdown menu. +
    • +
  • +Click Apply settings. +
    • +
+Solution (programmatically) +
+app <- iSEE(sce, initial = list(
+  ReducedDimensionPlot(PanelWidth = 3L),
+  RowDataPlot(PanelWidth = 4L),
+  ColumnDataPlot(PanelWidth = 5L)
+))
+

+
+
+

Recipe 2: Data parameters configuration - selecting columns to +display +

+

Using the pbmc3k data set, visualize the cell type +assignment against the cluster membership, with the aim to identify the +predominant cell type in each cluster. In this case, since both cell +annotations are categorical, iSEE will generate a so called +Hinton plot.

+
+Hints +
    +
  • +Column (in this case cell) annotations can be visualized using a +ColumnDataPlot panel. +
    • +
  • +The cluster labels are available in the Cluster column of +colData(sce). +
    • +
  • +The cell type assignments are available in the labels_fine +column of colData(sce) (more coarse-grained assignments are +provided in the labels_main column). +
    • +
  • +iSEE will automatically determine the plot type depending +on the type of selected variables. +
    • +
+Solution (interactively) +
    +
  • +First open an application with a single ColumnDataPlot +panel spanning the full application window) +
    • +
    +app <- iSEE(sce, initial = list(ColumnDataPlot(PanelWidth = 12L)))
+shiny::runApp(app)
    +
  • +In the ColumnDataPlot panel, click to expand the +Data parameters collapsible box. +
    • +
  • +Under Column of interest (Y-axis), select the label column +(e.g., labels_fine). +
    • +
  • +Under X-axis, select Column data, and under +Column of interest (X-axis), select Cluster. +
    • +
+Solution (programmatically) +
+app <- iSEE(sce, list(
+  ColumnDataPlot(
+    PanelWidth = 12L, XAxis = "Column data",
+    YAxis = "labels_fine", XAxisColumnData = "Cluster"
+  )
+))
+

+
+
+

Recipe 3: Data parameters configuration - displaying multiple +reduced dimension representations +

+

Using the pbmc3k data set, display both the tSNE and +UMAP representations next to each other.

+
+Hints +
    +
  • +Reduced dimension representations can be displayed using +ReducedDimensionPlot panels. +
    • +
  • +The reduced dimension representations in the SingleCellExperiment object +can be accessed by name (reducedDimNames(sce) lists the +available representations). +
    • +
+Solution (interactively) +
    +
  • +First open an application with two ReducedDimensionPlot +panels, each spanning half the application window) +
    • +
    +app <- iSEE(sce, initial = list(
+  ReducedDimensionPlot(PanelWidth = 6L),
+  ReducedDimensionPlot(PanelWidth = 6L)
+))
+shiny::runApp(app)
    +
  • +In the first ReducedDimensionPlot panel, click to expand +the Data parameters collapsible box. +
    • +
  • +In the Type selection box, choose TSNE. +
    • +
  • +In the second ReducedDimensionPlot panel, repeat the +procedure but instead select UMAP. +
    • +
+Solution (programmatically) +
+app <- iSEE(sce, initial = list(
+  ReducedDimensionPlot(PanelWidth = 6L, Type = "TSNE"),
+  ReducedDimensionPlot(PanelWidth = 6L, Type = "UMAP")
+))
+

+
+
+

Recipe 4: Data parameters configuration - displaying the expression +of a specific gene across clusters +

+

Using the pbmc3k data set, plot the distribution of the +logcount values for the gene CD74 in each of the +clusters.

+
+Hints +
    +
  • +Gene expression values can be displayed using a +FeatureAssayPlot panel. +
    • +
  • +To select a gene, specify the ID provided as row names in the +SingleCellExperiment. +
    • +
+Solution (interactively) +
    +
  • +First open an application with a FeatureAssayPlot panel, +spanning the full application window) +
    • +
    +app <- iSEE(sce, initial = list(FeatureAssayPlot(PanelWidth = 12L)))
+shiny::runApp(app)
    +
  • +In the FeatureAssayPlot panel, click to expand the +Data parameters collapsible box. +
    • +
  • +Under Y-axis feature, type or select CD74. +
    • +
  • +Under X-axis, select Column data, and under +Column of interest (X-axis), select Cluster. +
    • +
+Solution (programmatically) +
+app <- iSEE(sce, initial = list(
+  FeatureAssayPlot(
+    PanelWidth = 12L, XAxis = "Column data",
+    YAxisFeatureName = "CD74", XAxisColumnData = "Cluster"
+  )
+))
+

+
+
+

Recipe 5: Visual parameters configuration - coloring reduced +dimension representations by cell annotation +

+

Using the pbmc3k data set, display two tSNE +representations next to each other. In the first one, color the cells by +the cluster label. In the second one, color the cells by the log10 of +the total UMI count (log10_total column of +colData(sce)).

+
+Hints +
    +
  • +Reduced dimension representations can be displayed using +ReducedDimensionPlot panels. +
    • +
  • +The reduced dimension representations in the SingleCellExperiment object +can be accessed by name (reducedDimNames(sce) lists the +available representations). +
    • +
  • +The cluster labels are available in the Cluster column of +colData(sce). +
    • +
  • +Point attributes can be accessed in the Visual parameters +collapsible box. To display or hide the possible options, check the +corresponding checkboxes (Color, Shape, +Size, Point). +
    • +
+Solution (interactively) +
    +
  • +First open an application with two ReducedDimensionPlot +panels, each spanning half the application window) +
    • +
    +app <- iSEE(sce, initial = list(
+  ReducedDimensionPlot(PanelWidth = 6L),
+  ReducedDimensionPlot(PanelWidth = 6L)
+))
+shiny::runApp(app)
    +
  • +In each ReducedDimensionPlot panel, click to expand the +Data parameters collapsible box and under +Type, choose TSNE. +
    • +
  • +In the first ReducedDimensionPlot panel, click to expand +the Visual parameters collapsible box. +
    • +
  • +Make sure that the Color checkbox is ticked. +
    • +
  • +Under Color by, select Column data. +
    • +
  • +In the dropdown menu that appears, select Cluster. +
    • +
  • +In the second ReducedDimensionPlot panel, repeat the +procedure but instead select log10_total. +
    • +
+Solution (programmatically) +
+app <- iSEE(sce, initial = list(
+  ReducedDimensionPlot(
+    PanelWidth = 6L, Type = "TSNE",
+    ColorBy = "Column data",
+    ColorByColumnData = "Cluster"
+  ),
+  ReducedDimensionPlot(
+    PanelWidth = 6L, Type = "TSNE",
+    ColorBy = "Column data",
+    ColorByColumnData = "log10_total"
+  )
+))
+

+
+
+

Recipe 6: Visual parameters configuration - coloring reduced +dimension representations by gene expression +

+

Using the pbmc3k data set, display two tSNE +representations next to each other. In the first one, color the cells by +the logcounts expression level of CD3D. In the second one, +color the cells by the logcounts expression level of +CD79B.

+
+Hints +
    +
  • +Reduced dimension representations can be displayed using +ReducedDimensionPlot panels. +
    • +
  • +The reduced dimension representations in the SingleCellExperiment object +can be accessed by name (reducedDimNames(sce) lists the +available representations). +
    • +
  • +To select a gene, specify the ID provided as row names in the +SingleCellExperiment. +
    • +
  • +Point attributes can be accessed in the Visual parameters +collapsible box. To display or hide the possible options, check the +corresponding checkboxes (Color, Shape, +Size, Point). +
    • +
+Solution (interactively) +
    +
  • +First open an application with two ReducedDimensionPlot +panels, each spanning half the application window) +
    • +
    +app <- iSEE(sce, initial = list(
+  ReducedDimensionPlot(PanelWidth = 6L),
+  ReducedDimensionPlot(PanelWidth = 6L)
+))
+shiny::runApp(app)
    +
  • +In each ReducedDimensionPlot panel, click to expand the +Data parameters collapsible box and under +Type, choose TSNE. +
    • +
  • +In the first ReducedDimensionPlot panel, click to expand +the Visual parameters collapsible box. +
    • +
  • +Make sure that the Color checkbox is ticked. +
    • +
  • +Under Color by, select Feature name. +
    • +
  • +In the dropdown menu that appears, select or type CD3D. +
    • +
  • +In the second ReducedDimensionPlot panel, repeat the +procedure but instead select CD79B. +
    • +
+Solution (programmatically) +
+app <- iSEE(sce, initial = list(
+  ReducedDimensionPlot(
+    PanelWidth = 6L, Type = "TSNE",
+    ColorBy = "Feature name",
+    ColorByFeatureName = "CD3D"
+  ),
+  ReducedDimensionPlot(
+    PanelWidth = 6L, Type = "TSNE",
+    ColorBy = "Feature name",
+    ColorByFeatureName = "CD79B"
+  )
+))
+

+
+
+

Recipe 7: Visual parameters configuration - changing the size and +opacity of points +

+

Using the pbmc3k data set, display two tSNE +representations next to each other. In the first one, set the point size +to 0.5. In the second one, set the point size to 3 and the opacity to +0.2.

+
+Hints +
    +
  • +Reduced dimension representations can be displayed using +ReducedDimensionPlot panels. +
    • +
  • +The reduced dimension representations in the SingleCellExperiment object +can be accessed by name (reducedDimNames(sce) lists the +available representations). +
    • +
  • +Point attributes can be accessed in the Visual parameters +collapsible box. To display or hide the possible options, check the +corresponding checkboxes (Color, Shape, +Size, Point). +
    • +
+Solution (interactively) +
    +
  • +First open an application with two ReducedDimensionPlot +panels, each spanning half the application window) +
    • +
    +app <- iSEE(sce, initial = list(
+  ReducedDimensionPlot(PanelWidth = 6L),
+  ReducedDimensionPlot(PanelWidth = 6L)
+))
+shiny::runApp(app)
    +
  • +In each ReducedDimensionPlot panel, click to expand the +Data parameters collapsible box and under +Type, choose TSNE. +
    • +
  • +In the first ReducedDimensionPlot panel, click to expand +the Visual parameters collapsible box. +
    • +
  • +Make sure that the Size checkbox is ticked. +
    • +
  • +Under Size by, select None. +
    • +
  • +In the text box underneath, type 0.5. +
    • +
  • +In the second ReducedDimensionPlot panel, click to expand +the Visual parameters collapsible box. +
    • +
  • +Make sure that the Size and Point checkboxes +are ticked. +
    • +
  • +Under Size by, select None. +
    • +
  • +In the text box underneath, type 3. +
    • +
  • +Under Point opacity, drag the slider to 0.2. +
    • +
+Solution (programmatically) +
+app <- iSEE(sce, initial = list(
+  ReducedDimensionPlot(
+    PanelWidth = 6L, Type = "TSNE",
+    PointSize = 0.5
+  ),
+  ReducedDimensionPlot(
+    PanelWidth = 6L, Type = "TSNE",
+    PointSize = 3, PointAlpha = 0.2
+  )
+))
+

+
+
+

Recipe 8: Selection configuration +

+

Using the pbmc3k data set, display the expression +(logcounts) of CD3D across the assigned clusters, as well +as a tSNE representation colored by the cluster label. Select all cells +with a logcount expression value of CD3D between +(approximately) 0.5 and 4, and highlight these in the tSNE plot by means +of transparency.

+
+Hints +
    +
  • +Reduced dimension representations can be displayed using +ReducedDimensionPlot panels. +
    • +
  • +The reduced dimension representations in the SingleCellExperiment object +can be accessed by name (reducedDimNames(sce) lists the +available representations). +
    • +
  • +Gene expression values can be displayed using a +FeatureAssayPlot panel. +
    • +
  • +To select a gene, specify the ID provided as row names in the +SingleCellExperiment. +
    • +
  • +Transmission of selections is set up in the +Selection parameters collapsible box. +
    • +
  • +Points can be selected by clicking and dragging the mouse to draw a +rectangle around them, or by repeatedly clicking to make a lasso +(free-form) selection. +
    • +
+Solution (interactively) +
    +
  • +First open an application with one FeatureAssayPlot panel +and one ReducedDimensionPlot panel, each spanning half the +application window) +
    • +
    +app <- iSEE(sce, initial = list(
+  FeatureAssayPlot(PanelWidth = 6L),
+  ReducedDimensionPlot(PanelWidth = 6L)
+))
+shiny::runApp(app)
    +
  • +In the FeatureAssayPlot panel, click to expand the +Data parameters collapsible box and under +Y-axis feature, type or select CD3D. +
    • +
  • +Under X-axis, select Column data, and under +X-axis column data, select Cluster. +
    • +
  • +In the FeatureAssayPlot panel, use the mouse to drag a +rectangle around all points with a logcount expression value (y-axis) +between approximately 0.5 and 4. +
    • +
  • +In the ReducedDimensionPlot panel, click to expand the +Data parameters collapsible box and under +Type, choose TSNE. +
    • +
  • +In the ReducedDimensionPlot panel, further click to expand +the Visual parameters collapsible box, make sure that the +Color checkbox is ticked. Under Color by, +select Column data. In the dropdown menu that appears, type +or select Cluster. +
    • +
  • +In the ReducedDimensionPlot panel, click to expand the +Selection parameters collapsible box. +
    • +
  • +Under Receive column selection from, select +Feature assay plot 1. +
    • +
  • +Under Selection effect, make sure that +Transparent is chosen. +
    • +
+Solution (programmatically) +
+app <- iSEE(sce, initial = list(
+  FeatureAssayPlot(
+    PanelWidth = 6L,
+    BrushData = list(
+      xmin = 0, xmax = 15,
+      ymin = 0.5, ymax = 4,
+      mapping = list(x = "X", y = "Y", group = "GroupBy"),
+      direction = "xy",
+      brushId = "FeatureAssayPlot1_Brush",
+      outputId = "FeatureAssayPlot1"
+    ),
+    XAxis = "Column data",
+    XAxisColumnData = "Cluster", YAxisFeatureName = "CD3D"
+  ),
+  ReducedDimensionPlot(
+    PanelWidth = 6L, Type = "TSNE",
+    ColorBy = "Column data",
+    ColorByColumnData = "Cluster",
+    SelectionEffect = "Transparent",
+    ColumnSelectionSource = "FeatureAssayPlot1"
+  )
+))
+#> Warning in .local(.Object, ...): 'SelectionEffect=' is deprecated.
+#> Use 'ColumnSelectionRestrict=TRUE' instead.
+

+
+
+

Recipe 9: Verifying the cell type identity of clusters +

+

Using the pbmc3k data set, create a scatter plots +displaying the (logcounts) expression values of CD79A vs +CD74, as well as a Hinton plot of the cluster and cell type +assignment annotations. Select the cells co-expressing +CD79A and CD74 in the scatter plot. Which cell +type/cluster(s) do these correspond to (color these points in the Hinton +plot)?

+
+Hints +
    +
  • +Gene expression values can be displayed using a +FeatureAssayPlot panel. +
    • +
  • +To select a gene, specify the ID provided as row names in the +SingleCellExperiment. +
    • +
  • +Column (in this case cell) annotations can be visualized using a +ColumnDataPlot panel. +
    • +
  • +The cluster labels are available in the Cluster column of +colData(sce). Coarse-grained cell type labels are available +in the labels_main column. +
    • +
  • +Transmission of selections is set up in the +Selection parameters collapsible box. +
    • +
  • +Points can be selected by clicking and dragging the mouse to draw a +rectangle around them, or by repeatedly clicking to make a lasso +(free-form) selection. +
    • +
+Solution (interactively) +
    +
  • +First open an application with one FeatureAssayPlot panel +and one ColumnDataPlot panel, each spanning half of the +application window) +
    • +
    +app <- iSEE(sce, initial = list(
+  FeatureAssayPlot(PanelWidth = 6L),
+  ColumnDataPlot(PanelWidth = 6L)
+))
+shiny::runApp(app)
    +
  • +In the FeatureAssayPlot panel, click to expand the +Data parameters collapsible box. +
    • +
  • +Under Y-axis feature, type or select CD79A. +
    • +
  • +Under X-axis, select Feature name, and under +X-axis feature, type or select CD74. +
    • +
  • +In the ColumnDataPlot panel, click to expand the +Data parameters collapsible box. +
    • +
  • +Under Column of interest (Y-axis), type or select +labels_main. +
    • +
  • +Under X-axis, select Column data, and under +Column of interest (X-axis), select Cluster. +
    • +
  • +In the FeatureAssayPlot panel, use the mouse to drag a +rectangle around all points co-expressing CD79A and +CD74. +
    • +
  • +In the ColumnDataPlot panel, click to expand the +Selection parameters collapsible box. +
    • +
  • +Under Receive column selection from, select +Feature assay plot 1. +
    • +
  • +Under Selection effect, make sure that Color +is chosen. +
    • +
+Solution (programmatically) +
+app <- iSEE(sce, initial = list(
+  FeatureAssayPlot(
+    PanelWidth = 6L, XAxis = "Feature name",
+    YAxisFeatureName = "CD79A",
+    XAxisFeatureName = "CD74",
+    BrushData = list(
+      xmin = 0.3, xmax = 7,
+      ymin = 0.3, ymax = 7,
+      mapping = list(x = "X", y = "Y", colour = "ColorBy"),
+      direction = "xy", brushId = "FeatureAssayPlot1_Brush",
+      outputId = "FeatureAssayPlot1"
+    )
+  ),
+  ColumnDataPlot(
+    PanelWidth = 6L, XAxis = "Column data",
+    YAxis = "labels_main",
+    XAxisColumnData = "Cluster",
+    ColumnSelectionSource = "FeatureAssayPlot1",
+    ColumnSelectionRestrict = FALSE
+  )
+))
+

+
+
+

Recipe 10: Using modes from iSEEu +

+

Using the pbmc3k data set, load iSEEu and +use the modeReducedDim mode to open an app displaying all +the reduced dimension representations stored in the SingleCellExperiment +object. Color the representations by the cell type assignment.

+
+Hints +
    +
  • +The cell type assignments are available in the labels_fine +column of colData(sce) (more coarse-grained assignments are +provided in the labels_main column). +
    • +
  • +The annotation to color by can be specified when calling +iSEEu::modeReducedDim(). +
    • +
+Solution (programmatically) +
+app <- modeReducedDim(sce, colorBy = "labels_main")
+

+
+
+

Recipe 11 - Including a tour +

+

Using the pbmc3k data set, display two tSNE +representations next to each other. In the first one, color the cells by +the logcounts expression level of CD3D. In the second one, +color the cells by the logcounts expression level of CD79B. +Also include a small tour that starts with a welcome message, next walks +through the two panels, giving an informative message for each, and +finally ends with a concluding message to the user.

+
+Hints +
    +
  • +Reduced dimension representations can be displayed using +ReducedDimensionPlot panels. +
    • +
  • +The reduced dimension representations in the SingleCellExperiment object +can be accessed by name (reducedDimNames(sce) lists the +available representations). +
    • +
  • +To select a gene, specify the ID provided as row names in the +SingleCellExperiment. +
    • +
  • +Point attributes can be accessed in the Visual parameters +collapsible box. To display or hide the possible options, check the +corresponding checkboxes (Color, Shape, +Size, Point). +
    • +
  • +Tours are provided via the tour argument to +iSEE(). +
    • +
  • +A tour is defined by a two-column data frame, with columns named +element and intro. The element +column contains the names of UI elements, prefixed by a hash sign. More +details, including how to find the name of a particular UI elements, can +be found in the Configuring iSEE apps vignette of +iSEE. +
    • +
+Solution (programmatically) +
+tour <- data.frame(
+  element = c(
+    "#Welcome",
+    "#ReducedDimensionPlot1",
+    "#ReducedDimensionPlot2",
+    "#Conclusion"
+  ),
+  intro = c(
+    "Welcome to this tour!",
+    "This is the first reduced dimension plot",
+    "And here is the second one",
+    "Thank you for taking this tour!"
+  ),
+  stringsAsFactors = FALSE
+)
+
+app <- iSEE(sce,
+  initial = list(
+    ReducedDimensionPlot(
+      PanelWidth = 6L, Type = "TSNE",
+      ColorBy = "Feature name",
+      ColorByFeatureName = "CD3D"
+    ),
+    ReducedDimensionPlot(
+      PanelWidth = 6L, Type = "TSNE",
+      ColorBy = "Feature name",
+      ColorByFeatureName = "CD79B"
+    )
+  ),
+  tour = tour
+)
+

+
+
+
+

Session info +

+
+sessionInfo()
+#> R version 4.4.0 (2024-04-24)
+#> Platform: x86_64-apple-darwin20
+#> Running under: macOS Monterey 12.7.1
+#> 
+#> Matrix products: default
+#> BLAS:   /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRblas.0.dylib 
+#> LAPACK: /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRlapack.dylib;  LAPACK version 3.12.0
+#> 
+#> locale:
+#> [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
+#> 
+#> time zone: Europe/Berlin
+#> tzcode source: internal
+#> 
+#> attached base packages:
+#> [1] stats4    stats     graphics  grDevices utils     datasets  methods  
+#> [8] base     
+#> 
+#> other attached packages:
+#>  [1] iSEEu_1.15.1                iSEEhex_1.5.0              
+#>  [3] iSEE_2.15.1                 SingleCellExperiment_1.26.0
+#>  [5] SummarizedExperiment_1.33.3 Biobase_2.64.0             
+#>  [7] GenomicRanges_1.55.4        GenomeInfoDb_1.40.0        
+#>  [9] IRanges_2.38.0              S4Vectors_0.42.0           
+#> [11] BiocGenerics_0.50.0         MatrixGenerics_1.16.0      
+#> [13] matrixStats_1.3.0           BiocStyle_2.32.0           
+#> 
+#> loaded via a namespace (and not attached):
+#>   [1] bitops_1.0-7            rlang_1.1.3             magrittr_2.0.3         
+#>   [4] shinydashboard_0.7.2    clue_0.3-65             GetoptLong_1.0.5       
+#>   [7] compiler_4.4.0          mgcv_1.9-1              png_0.1-8              
+#>  [10] systemfonts_1.0.6       vctrs_0.6.5             pkgconfig_2.0.3        
+#>  [13] shape_1.4.6.1           crayon_1.5.2            fastmap_1.1.1          
+#>  [16] XVector_0.44.0          fontawesome_0.5.2       utf8_1.2.4             
+#>  [19] promises_1.3.0          rmarkdown_2.26          shinyAce_0.4.2         
+#>  [22] UCSC.utils_1.0.0        ragg_1.3.0              purrr_1.0.2            
+#>  [25] xfun_0.43               zlibbioc_1.50.0         cachem_1.0.8           
+#>  [28] jsonlite_1.8.8          listviewer_4.0.0        highr_0.10             
+#>  [31] later_1.3.2             DelayedArray_0.30.0     parallel_4.4.0         
+#>  [34] cluster_2.1.6           R6_2.5.1                bslib_0.7.0            
+#>  [37] RColorBrewer_1.1-3      jquerylib_0.1.4         Rcpp_1.0.12            
+#>  [40] iterators_1.0.14        knitr_1.46              httpuv_1.6.15          
+#>  [43] Matrix_1.7-0            splines_4.4.0           igraph_2.0.3           
+#>  [46] tidyselect_1.2.1        rstudioapi_0.16.0       abind_1.4-5            
+#>  [49] yaml_2.3.8              doParallel_1.0.17       codetools_0.2-20       
+#>  [52] miniUI_0.1.1.1          lattice_0.22-6          tibble_3.2.1           
+#>  [55] shiny_1.8.1.1           evaluate_0.23           desc_1.4.3             
+#>  [58] circlize_0.4.16         pillar_1.9.0            BiocManager_1.30.22    
+#>  [61] DT_0.33                 foreach_1.5.2           shinyjs_2.1.0          
+#>  [64] generics_0.1.3          RCurl_1.98-1.14         ggplot2_3.5.1          
+#>  [67] munsell_0.5.1           scales_1.3.0            xtable_1.8-4           
+#>  [70] glue_1.7.0              tools_4.4.0             hexbin_1.28.3          
+#>  [73] colourpicker_1.3.0      fs_1.6.4                grid_4.4.0             
+#>  [76] colorspace_2.1-0        nlme_3.1-164            GenomeInfoDbData_1.2.12
+#>  [79] vipor_0.4.7             cli_3.6.2               textshaping_0.3.7      
+#>  [82] fansi_1.0.6             viridisLite_0.4.2       S4Arrays_1.4.0         
+#>  [85] ComplexHeatmap_2.20.0   dplyr_1.1.4             gtable_0.3.5           
+#>  [88] rintrojs_0.3.4          sass_0.4.9              digest_0.6.35          
+#>  [91] SparseArray_1.4.0       ggrepel_0.9.5           rjson_0.2.21           
+#>  [94] htmlwidgets_1.6.4       memoise_2.0.1           htmltools_0.5.8.1      
+#>  [97] pkgdown_2.0.9           lifecycle_1.0.4         shinyWidgets_0.8.6     
+#> [100] httr_1.4.7              GlobalOptions_0.1.2     mime_0.12
+
+
+

References +

+
+
+Rue-Albrecht, Kevin, Federico Marini, Charlotte Soneson, and Aaron T L +Lun. 2018. “iSEE: Interactive +SummarizedExperiment Explorer.” F1000Res. 7 +(June). +
+
+
+
+
+
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+ + + +
+ + + + +
+ + + + + + + + diff --git a/articles/iSEE_quickrecipes_03_files/font-awesome-6.4.2/css/all.css b/articles/iSEE_quickrecipes_03_files/font-awesome-6.4.2/css/all.css new file mode 100644 index 0000000..bdb6e3a --- /dev/null +++ b/articles/iSEE_quickrecipes_03_files/font-awesome-6.4.2/css/all.css @@ -0,0 +1,7968 @@ +/*! + * Font Awesome Free 6.4.2 by @fontawesome - https://fontawesome.com + * License - https://fontawesome.com/license/free (Icons: CC BY 4.0, Fonts: SIL OFL 1.1, Code: MIT License) + * Copyright 2023 Fonticons, Inc. + */ +.fa { + font-family: var(--fa-style-family, "Font Awesome 6 Free"); + font-weight: var(--fa-style, 900); } + +.fa, +.fa-classic, +.fa-sharp, +.fas, +.fa-solid, +.far, +.fa-regular, +.fab, +.fa-brands { + -moz-osx-font-smoothing: grayscale; + -webkit-font-smoothing: antialiased; + display: var(--fa-display, inline-block); + font-style: normal; + font-variant: normal; + line-height: 1; + text-rendering: auto; } + +.fas, +.fa-classic, +.fa-solid, +.far, +.fa-regular { + font-family: 'Font Awesome 6 Free'; } + +.fab, +.fa-brands { + font-family: 'Font Awesome 6 Brands'; } + +.fa-1x { + font-size: 1em; } + +.fa-2x { + font-size: 2em; } + +.fa-3x { + font-size: 3em; } + +.fa-4x { + font-size: 4em; } + +.fa-5x { + font-size: 5em; } + +.fa-6x { + font-size: 6em; } + +.fa-7x { + font-size: 7em; } + +.fa-8x { + font-size: 8em; } + +.fa-9x { + font-size: 9em; } + +.fa-10x { + font-size: 10em; } + +.fa-2xs { + font-size: 0.625em; + line-height: 0.1em; + vertical-align: 0.225em; } + +.fa-xs { + font-size: 0.75em; + line-height: 0.08333em; + vertical-align: 0.125em; } + +.fa-sm { + font-size: 0.875em; + line-height: 0.07143em; + vertical-align: 0.05357em; } + +.fa-lg { + font-size: 1.25em; + line-height: 0.05em; + vertical-align: -0.075em; } + +.fa-xl { + font-size: 1.5em; + line-height: 0.04167em; + vertical-align: -0.125em; } + +.fa-2xl { + font-size: 2em; + line-height: 0.03125em; + vertical-align: -0.1875em; } + +.fa-fw { + text-align: center; + width: 1.25em; } + +.fa-ul { + list-style-type: none; + margin-left: var(--fa-li-margin, 2.5em); + padding-left: 0; } + .fa-ul > li { + position: relative; } + +.fa-li { + left: calc(var(--fa-li-width, 2em) * -1); + position: absolute; + text-align: center; + width: var(--fa-li-width, 2em); + line-height: inherit; } + +.fa-border { + border-color: var(--fa-border-color, #eee); + border-radius: var(--fa-border-radius, 0.1em); + border-style: var(--fa-border-style, solid); + border-width: var(--fa-border-width, 0.08em); + padding: var(--fa-border-padding, 0.2em 0.25em 0.15em); } + +.fa-pull-left { + float: left; + margin-right: var(--fa-pull-margin, 0.3em); } + +.fa-pull-right { + float: right; + margin-left: var(--fa-pull-margin, 0.3em); } + +.fa-beat { + -webkit-animation-name: fa-beat; + animation-name: fa-beat; + -webkit-animation-delay: var(--fa-animation-delay, 0s); + animation-delay: var(--fa-animation-delay, 0s); + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 1s); + animation-duration: var(--fa-animation-duration, 1s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, ease-in-out); + animation-timing-function: var(--fa-animation-timing, ease-in-out); } + +.fa-bounce { + -webkit-animation-name: fa-bounce; + animation-name: fa-bounce; + -webkit-animation-delay: var(--fa-animation-delay, 0s); + animation-delay: var(--fa-animation-delay, 0s); + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 1s); + animation-duration: var(--fa-animation-duration, 1s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, cubic-bezier(0.28, 0.84, 0.42, 1)); + animation-timing-function: var(--fa-animation-timing, cubic-bezier(0.28, 0.84, 0.42, 1)); } + +.fa-fade { + -webkit-animation-name: fa-fade; + animation-name: fa-fade; + -webkit-animation-delay: var(--fa-animation-delay, 0s); + animation-delay: var(--fa-animation-delay, 0s); + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 1s); + animation-duration: var(--fa-animation-duration, 1s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, cubic-bezier(0.4, 0, 0.6, 1)); + animation-timing-function: var(--fa-animation-timing, cubic-bezier(0.4, 0, 0.6, 1)); } + +.fa-beat-fade { + -webkit-animation-name: fa-beat-fade; + animation-name: fa-beat-fade; + -webkit-animation-delay: var(--fa-animation-delay, 0s); + animation-delay: var(--fa-animation-delay, 0s); + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 1s); + animation-duration: var(--fa-animation-duration, 1s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, cubic-bezier(0.4, 0, 0.6, 1)); + animation-timing-function: var(--fa-animation-timing, cubic-bezier(0.4, 0, 0.6, 1)); } + +.fa-flip { + -webkit-animation-name: fa-flip; + animation-name: fa-flip; + -webkit-animation-delay: var(--fa-animation-delay, 0s); + animation-delay: var(--fa-animation-delay, 0s); + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 1s); + animation-duration: var(--fa-animation-duration, 1s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, ease-in-out); + animation-timing-function: var(--fa-animation-timing, ease-in-out); } + +.fa-shake { + -webkit-animation-name: fa-shake; + animation-name: fa-shake; + -webkit-animation-delay: var(--fa-animation-delay, 0s); + animation-delay: var(--fa-animation-delay, 0s); + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 1s); + animation-duration: var(--fa-animation-duration, 1s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, linear); + animation-timing-function: var(--fa-animation-timing, linear); } + +.fa-spin { + -webkit-animation-name: fa-spin; + animation-name: fa-spin; + -webkit-animation-delay: var(--fa-animation-delay, 0s); + animation-delay: var(--fa-animation-delay, 0s); + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 2s); + animation-duration: var(--fa-animation-duration, 2s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, linear); + animation-timing-function: var(--fa-animation-timing, linear); } + +.fa-spin-reverse { + --fa-animation-direction: reverse; } + +.fa-pulse, +.fa-spin-pulse { + -webkit-animation-name: fa-spin; + animation-name: fa-spin; + -webkit-animation-direction: var(--fa-animation-direction, normal); + animation-direction: var(--fa-animation-direction, normal); + -webkit-animation-duration: var(--fa-animation-duration, 1s); + animation-duration: var(--fa-animation-duration, 1s); + -webkit-animation-iteration-count: var(--fa-animation-iteration-count, infinite); + animation-iteration-count: var(--fa-animation-iteration-count, infinite); + -webkit-animation-timing-function: var(--fa-animation-timing, steps(8)); + animation-timing-function: var(--fa-animation-timing, steps(8)); } + +@media (prefers-reduced-motion: reduce) { + .fa-beat, + .fa-bounce, + .fa-fade, + .fa-beat-fade, + .fa-flip, + .fa-pulse, + .fa-shake, + .fa-spin, + .fa-spin-pulse { + -webkit-animation-delay: -1ms; + animation-delay: -1ms; + -webkit-animation-duration: 1ms; + animation-duration: 1ms; + -webkit-animation-iteration-count: 1; + animation-iteration-count: 1; + -webkit-transition-delay: 0s; + transition-delay: 0s; + -webkit-transition-duration: 0s; + transition-duration: 0s; } } + +@-webkit-keyframes fa-beat { + 0%, 90% { + -webkit-transform: scale(1); + transform: scale(1); } + 45% { + -webkit-transform: scale(var(--fa-beat-scale, 1.25)); + transform: scale(var(--fa-beat-scale, 1.25)); } } + +@keyframes fa-beat { + 0%, 90% { + -webkit-transform: scale(1); + transform: scale(1); } + 45% { + -webkit-transform: scale(var(--fa-beat-scale, 1.25)); + transform: scale(var(--fa-beat-scale, 1.25)); } } + +@-webkit-keyframes fa-bounce { + 0% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } + 10% { + -webkit-transform: scale(var(--fa-bounce-start-scale-x, 1.1), var(--fa-bounce-start-scale-y, 0.9)) translateY(0); + transform: scale(var(--fa-bounce-start-scale-x, 1.1), var(--fa-bounce-start-scale-y, 0.9)) translateY(0); } + 30% { + -webkit-transform: scale(var(--fa-bounce-jump-scale-x, 0.9), var(--fa-bounce-jump-scale-y, 1.1)) translateY(var(--fa-bounce-height, -0.5em)); + transform: scale(var(--fa-bounce-jump-scale-x, 0.9), var(--fa-bounce-jump-scale-y, 1.1)) translateY(var(--fa-bounce-height, -0.5em)); } + 50% { + -webkit-transform: scale(var(--fa-bounce-land-scale-x, 1.05), var(--fa-bounce-land-scale-y, 0.95)) translateY(0); + transform: scale(var(--fa-bounce-land-scale-x, 1.05), var(--fa-bounce-land-scale-y, 0.95)) translateY(0); } + 57% { + -webkit-transform: scale(1, 1) translateY(var(--fa-bounce-rebound, -0.125em)); + transform: scale(1, 1) translateY(var(--fa-bounce-rebound, -0.125em)); } + 64% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } + 100% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } } + +@keyframes fa-bounce { + 0% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } + 10% { + -webkit-transform: scale(var(--fa-bounce-start-scale-x, 1.1), var(--fa-bounce-start-scale-y, 0.9)) translateY(0); + transform: scale(var(--fa-bounce-start-scale-x, 1.1), var(--fa-bounce-start-scale-y, 0.9)) translateY(0); } + 30% { + -webkit-transform: scale(var(--fa-bounce-jump-scale-x, 0.9), var(--fa-bounce-jump-scale-y, 1.1)) translateY(var(--fa-bounce-height, -0.5em)); + transform: scale(var(--fa-bounce-jump-scale-x, 0.9), var(--fa-bounce-jump-scale-y, 1.1)) translateY(var(--fa-bounce-height, -0.5em)); } + 50% { + -webkit-transform: scale(var(--fa-bounce-land-scale-x, 1.05), var(--fa-bounce-land-scale-y, 0.95)) translateY(0); + transform: scale(var(--fa-bounce-land-scale-x, 1.05), var(--fa-bounce-land-scale-y, 0.95)) translateY(0); } + 57% { + -webkit-transform: scale(1, 1) translateY(var(--fa-bounce-rebound, -0.125em)); + transform: scale(1, 1) translateY(var(--fa-bounce-rebound, -0.125em)); } + 64% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } + 100% { + -webkit-transform: scale(1, 1) translateY(0); + transform: scale(1, 1) translateY(0); } } + +@-webkit-keyframes fa-fade { + 50% { + opacity: var(--fa-fade-opacity, 0.4); } } + +@keyframes fa-fade { + 50% { + opacity: var(--fa-fade-opacity, 0.4); } } + +@-webkit-keyframes fa-beat-fade { + 0%, 100% { + opacity: var(--fa-beat-fade-opacity, 0.4); + -webkit-transform: scale(1); + transform: scale(1); } + 50% { + opacity: 1; + -webkit-transform: scale(var(--fa-beat-fade-scale, 1.125)); + transform: scale(var(--fa-beat-fade-scale, 1.125)); } } + +@keyframes fa-beat-fade { + 0%, 100% { + opacity: var(--fa-beat-fade-opacity, 0.4); + -webkit-transform: scale(1); + transform: scale(1); } + 50% { + opacity: 1; + -webkit-transform: scale(var(--fa-beat-fade-scale, 1.125)); + transform: scale(var(--fa-beat-fade-scale, 1.125)); } } + +@-webkit-keyframes fa-flip { + 50% { + -webkit-transform: rotate3d(var(--fa-flip-x, 0), var(--fa-flip-y, 1), var(--fa-flip-z, 0), var(--fa-flip-angle, -180deg)); + transform: rotate3d(var(--fa-flip-x, 0), var(--fa-flip-y, 1), var(--fa-flip-z, 0), var(--fa-flip-angle, -180deg)); } } + +@keyframes fa-flip { + 50% { + -webkit-transform: rotate3d(var(--fa-flip-x, 0), var(--fa-flip-y, 1), var(--fa-flip-z, 0), var(--fa-flip-angle, -180deg)); + transform: rotate3d(var(--fa-flip-x, 0), var(--fa-flip-y, 1), var(--fa-flip-z, 0), var(--fa-flip-angle, -180deg)); } } + +@-webkit-keyframes fa-shake { + 0% { + -webkit-transform: rotate(-15deg); + transform: rotate(-15deg); } + 4% { + -webkit-transform: rotate(15deg); + transform: rotate(15deg); } + 8%, 24% { + -webkit-transform: rotate(-18deg); + transform: rotate(-18deg); } + 12%, 28% { + -webkit-transform: rotate(18deg); + transform: rotate(18deg); } + 16% { + -webkit-transform: rotate(-22deg); + transform: rotate(-22deg); } + 20% { + -webkit-transform: rotate(22deg); + transform: rotate(22deg); } + 32% { + -webkit-transform: rotate(-12deg); + transform: rotate(-12deg); } + 36% { + -webkit-transform: rotate(12deg); + transform: rotate(12deg); } + 40%, 100% { + -webkit-transform: rotate(0deg); + transform: rotate(0deg); } } + +@keyframes fa-shake { + 0% { + -webkit-transform: rotate(-15deg); + transform: rotate(-15deg); } + 4% { + -webkit-transform: rotate(15deg); + transform: rotate(15deg); } + 8%, 24% { + -webkit-transform: rotate(-18deg); + transform: rotate(-18deg); } + 12%, 28% { + -webkit-transform: rotate(18deg); + transform: rotate(18deg); } + 16% { + -webkit-transform: rotate(-22deg); + transform: rotate(-22deg); } + 20% { + -webkit-transform: rotate(22deg); + transform: rotate(22deg); } + 32% { + -webkit-transform: rotate(-12deg); + transform: rotate(-12deg); } + 36% { + -webkit-transform: rotate(12deg); + transform: rotate(12deg); } + 40%, 100% { + -webkit-transform: rotate(0deg); + transform: rotate(0deg); } } + +@-webkit-keyframes fa-spin { + 0% { + -webkit-transform: rotate(0deg); + transform: rotate(0deg); } + 100% { + -webkit-transform: rotate(360deg); + transform: rotate(360deg); } } + +@keyframes fa-spin { + 0% { + -webkit-transform: rotate(0deg); + transform: rotate(0deg); } + 100% { + -webkit-transform: rotate(360deg); + transform: rotate(360deg); } } + +.fa-rotate-90 { + -webkit-transform: rotate(90deg); + transform: rotate(90deg); } + +.fa-rotate-180 { + -webkit-transform: rotate(180deg); + transform: rotate(180deg); } + +.fa-rotate-270 { + -webkit-transform: rotate(270deg); + transform: rotate(270deg); } + +.fa-flip-horizontal { + -webkit-transform: scale(-1, 1); + transform: scale(-1, 1); } + +.fa-flip-vertical { + -webkit-transform: scale(1, -1); + transform: scale(1, -1); } + +.fa-flip-both, +.fa-flip-horizontal.fa-flip-vertical { + -webkit-transform: scale(-1, -1); + transform: scale(-1, -1); } + +.fa-rotate-by { + -webkit-transform: rotate(var(--fa-rotate-angle, none)); + transform: rotate(var(--fa-rotate-angle, none)); } + +.fa-stack { + display: inline-block; + height: 2em; + line-height: 2em; + position: relative; + vertical-align: middle; + width: 2.5em; } + +.fa-stack-1x, +.fa-stack-2x { + left: 0; + position: absolute; + text-align: center; + width: 100%; + z-index: var(--fa-stack-z-index, auto); } + +.fa-stack-1x { + line-height: inherit; } + +.fa-stack-2x { + font-size: 2em; } + +.fa-inverse { + color: var(--fa-inverse, #fff); } + +/* Font Awesome uses the Unicode Private Use Area (PUA) to ensure screen +readers do not read off random characters that represent icons */ + +.fa-0::before { + content: "\30"; } + +.fa-1::before { + content: "\31"; } + +.fa-2::before { + content: "\32"; } + +.fa-3::before { + content: "\33"; } + +.fa-4::before { + content: "\34"; } + +.fa-5::before { + content: "\35"; } + +.fa-6::before { + content: "\36"; } + +.fa-7::before { + content: "\37"; } + +.fa-8::before { + content: "\38"; } + +.fa-9::before { + content: "\39"; } + +.fa-fill-drip::before { + content: "\f576"; } + +.fa-arrows-to-circle::before { + content: "\e4bd"; } + +.fa-circle-chevron-right::before { + content: "\f138"; } + +.fa-chevron-circle-right::before { + content: "\f138"; } + +.fa-at::before { + content: "\40"; } + +.fa-trash-can::before { + content: "\f2ed"; } + +.fa-trash-alt::before { + content: "\f2ed"; } + +.fa-text-height::before { + content: "\f034"; } + +.fa-user-xmark::before { + content: "\f235"; } + +.fa-user-times::before { + content: "\f235"; } + +.fa-stethoscope::before { + content: "\f0f1"; } + +.fa-message::before { + content: "\f27a"; } + +.fa-comment-alt::before { + content: "\f27a"; } + +.fa-info::before { + content: "\f129"; } + +.fa-down-left-and-up-right-to-center::before { + content: "\f422"; } + +.fa-compress-alt::before { + content: "\f422"; } + +.fa-explosion::before { + content: "\e4e9"; } + +.fa-file-lines::before { + content: "\f15c"; } + +.fa-file-alt::before { + content: "\f15c"; } + +.fa-file-text::before { + content: "\f15c"; } + +.fa-wave-square::before { + content: "\f83e"; } + +.fa-ring::before { + content: "\f70b"; } + +.fa-building-un::before { + content: "\e4d9"; } + +.fa-dice-three::before { + content: "\f527"; } + +.fa-calendar-days::before { + content: "\f073"; } + +.fa-calendar-alt::before { + content: "\f073"; } + +.fa-anchor-circle-check::before { + content: "\e4aa"; } + +.fa-building-circle-arrow-right::before { + content: "\e4d1"; } + +.fa-volleyball::before { + content: "\f45f"; } + +.fa-volleyball-ball::before { + content: "\f45f"; } + +.fa-arrows-up-to-line::before { + content: "\e4c2"; } + +.fa-sort-down::before { + content: "\f0dd"; } + +.fa-sort-desc::before { + content: "\f0dd"; } + +.fa-circle-minus::before { + content: "\f056"; } + +.fa-minus-circle::before { + content: "\f056"; } + +.fa-door-open::before { + content: "\f52b"; } + +.fa-right-from-bracket::before { + content: "\f2f5"; } + +.fa-sign-out-alt::before { + content: "\f2f5"; } + +.fa-atom::before { + content: "\f5d2"; } + +.fa-soap::before { + content: "\e06e"; } + +.fa-icons::before { + content: "\f86d"; } + +.fa-heart-music-camera-bolt::before { + content: "\f86d"; } + +.fa-microphone-lines-slash::before { + content: "\f539"; } + +.fa-microphone-alt-slash::before { + content: "\f539"; } + +.fa-bridge-circle-check::before { + content: "\e4c9"; } + +.fa-pump-medical::before { + content: "\e06a"; } + +.fa-fingerprint::before { + content: "\f577"; } + +.fa-hand-point-right::before { + content: "\f0a4"; } + +.fa-magnifying-glass-location::before { + content: "\f689"; } + +.fa-search-location::before { + content: "\f689"; } + +.fa-forward-step::before { + content: "\f051"; } + +.fa-step-forward::before { + content: "\f051"; } + +.fa-face-smile-beam::before { + content: "\f5b8"; } + +.fa-smile-beam::before { + content: "\f5b8"; } + 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+.fa-laptop::before { + content: "\f109"; } + +.fa-file-csv::before { + content: "\f6dd"; } + +.fa-menorah::before { + content: "\f676"; } + +.fa-truck-plane::before { + content: "\e58f"; } + +.fa-record-vinyl::before { + content: "\f8d9"; } + +.fa-face-grin-stars::before { + content: "\f587"; } + +.fa-grin-stars::before { + content: "\f587"; } + +.fa-bong::before { + content: "\f55c"; } + +.fa-spaghetti-monster-flying::before { + content: "\f67b"; } + +.fa-pastafarianism::before { + content: "\f67b"; } + +.fa-arrow-down-up-across-line::before { + content: "\e4af"; } + +.fa-spoon::before { + content: "\f2e5"; } + +.fa-utensil-spoon::before { + content: "\f2e5"; } + +.fa-jar-wheat::before { + content: "\e517"; } + +.fa-envelopes-bulk::before { + content: "\f674"; } + +.fa-mail-bulk::before { + content: "\f674"; } + +.fa-file-circle-exclamation::before { + content: "\e4eb"; } + +.fa-circle-h::before { + content: "\f47e"; } + +.fa-hospital-symbol::before { + content: "\f47e"; } + 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content: "\e445"; } + +.fa-window-restore::before { + content: "\f2d2"; } + +.fa-square-plus::before { + content: "\f0fe"; } + +.fa-plus-square::before { + content: "\f0fe"; } + +.fa-torii-gate::before { + content: "\f6a1"; } + +.fa-frog::before { + content: "\f52e"; } + +.fa-bucket::before { + content: "\e4cf"; } + +.fa-image::before { + content: "\f03e"; } + +.fa-microphone::before { + content: "\f130"; } + +.fa-cow::before { + content: "\f6c8"; } + +.fa-caret-up::before { + content: "\f0d8"; } + +.fa-screwdriver::before { + content: "\f54a"; } + +.fa-folder-closed::before { + content: "\e185"; } + +.fa-house-tsunami::before { + content: "\e515"; } + +.fa-square-nfi::before { + content: "\e576"; } + +.fa-arrow-up-from-ground-water::before { + content: "\e4b5"; } + +.fa-martini-glass::before { + content: "\f57b"; } + +.fa-glass-martini-alt::before { + content: "\f57b"; } + +.fa-rotate-left::before { + content: "\f2ea"; } + +.fa-rotate-back::before { + content: "\f2ea"; } + 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content: "\e5b4"; } + +.fa-user-nurse::before { + content: "\f82f"; } + +.fa-syringe::before { + content: "\f48e"; } + +.fa-cloud-sun::before { + content: "\f6c4"; } + +.fa-stopwatch-20::before { + content: "\e06f"; } + +.fa-square-full::before { + content: "\f45c"; } + +.fa-magnet::before { + content: "\f076"; } + +.fa-jar::before { + content: "\e516"; } + +.fa-note-sticky::before { + content: "\f249"; } + +.fa-sticky-note::before { + content: "\f249"; } + +.fa-bug-slash::before { + content: "\e490"; } + +.fa-arrow-up-from-water-pump::before { + content: "\e4b6"; } + +.fa-bone::before { + content: "\f5d7"; } + +.fa-user-injured::before { + content: "\f728"; } + +.fa-face-sad-tear::before { + content: "\f5b4"; } + +.fa-sad-tear::before { + content: "\f5b4"; } + +.fa-plane::before { + content: "\f072"; } + +.fa-tent-arrows-down::before { + content: "\e581"; } + +.fa-exclamation::before { + content: "\21"; } + +.fa-arrows-spin::before { + content: "\e4bb"; } + +.fa-print::before { + content: "\f02f"; } + +.fa-turkish-lira-sign::before { + content: "\e2bb"; } + +.fa-try::before { + content: "\e2bb"; } + +.fa-turkish-lira::before { + content: "\e2bb"; } + +.fa-dollar-sign::before { + content: "\24"; } + +.fa-dollar::before { + content: "\24"; } + +.fa-usd::before { + content: "\24"; } + +.fa-x::before { + content: "\58"; } + +.fa-magnifying-glass-dollar::before { + content: "\f688"; } + +.fa-search-dollar::before { + content: "\f688"; } + +.fa-users-gear::before { + content: "\f509"; } + +.fa-users-cog::before { + content: "\f509"; } + +.fa-person-military-pointing::before { + content: "\e54a"; } + +.fa-building-columns::before { + content: "\f19c"; } + +.fa-bank::before { + content: "\f19c"; } + +.fa-institution::before { + content: "\f19c"; } + +.fa-museum::before { + content: "\f19c"; } + +.fa-university::before { + content: "\f19c"; } + +.fa-umbrella::before { + content: "\f0e9"; } + +.fa-trowel::before { + content: "\e589"; } + +.fa-d::before { + content: 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"\e53e"; } + +.fa-turn-up::before { + content: "\f3bf"; } + +.fa-level-up-alt::before { + content: "\f3bf"; } + +.sr-only, +.fa-sr-only { + position: absolute; + width: 1px; + height: 1px; + padding: 0; + margin: -1px; + overflow: hidden; + clip: rect(0, 0, 0, 0); + white-space: nowrap; + border-width: 0; } + +.sr-only-focusable:not(:focus), +.fa-sr-only-focusable:not(:focus) { + position: absolute; + width: 1px; + height: 1px; + padding: 0; + margin: -1px; + overflow: hidden; + clip: rect(0, 0, 0, 0); + white-space: nowrap; + border-width: 0; } +:root, :host { + --fa-style-family-brands: 'Font Awesome 6 Brands'; + --fa-font-brands: normal 400 1em/1 'Font Awesome 6 Brands'; } + +@font-face { + font-family: 'Font Awesome 6 Brands'; + font-style: normal; + font-weight: 400; + font-display: block; + src: url("../webfonts/fa-brands-400.woff2") format("woff2"), url("../webfonts/fa-brands-400.ttf") format("truetype"); } + +.fab, +.fa-brands { + font-weight: 400; } + +.fa-monero:before { + content: "\f3d0"; } + +.fa-hooli:before { + content: "\f427"; } + +.fa-yelp:before { + content: "\f1e9"; } + +.fa-cc-visa:before { + content: "\f1f0"; } + +.fa-lastfm:before { + content: "\f202"; } + +.fa-shopware:before { + content: "\f5b5"; } + +.fa-creative-commons-nc:before { + content: "\f4e8"; } + +.fa-aws:before { + content: "\f375"; } + +.fa-redhat:before { + content: "\f7bc"; } + +.fa-yoast:before { + content: "\f2b1"; } + +.fa-cloudflare:before { + content: "\e07d"; } + +.fa-ups:before { + content: "\f7e0"; } + +.fa-wpexplorer:before { + content: "\f2de"; } + +.fa-dyalog:before { + content: "\f399"; } + +.fa-bity:before { + content: "\f37a"; } + +.fa-stackpath:before { + content: "\f842"; } + +.fa-buysellads:before { + content: "\f20d"; } + +.fa-first-order:before { + content: "\f2b0"; } + +.fa-modx:before { + content: "\f285"; } + +.fa-guilded:before { + content: "\e07e"; } + +.fa-vnv:before { + content: "\f40b"; } + +.fa-square-js:before { + content: "\f3b9"; } + 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+ +.fa.fa-linkedin-square:before { + content: "\f08c"; } + +.fa.fa-thumb-tack:before { + content: "\f08d"; } + +.fa.fa-external-link:before { + content: "\f35d"; } + +.fa.fa-sign-in:before { + content: "\f2f6"; } + +.fa.fa-github-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-github-square:before { + content: "\f092"; } + +.fa.fa-lemon-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-lemon-o:before { + content: "\f094"; } + +.fa.fa-square-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-square-o:before { + content: "\f0c8"; } + +.fa.fa-bookmark-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-bookmark-o:before { + content: "\f02e"; } + +.fa.fa-twitter { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-facebook { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-facebook:before { + content: "\f39e"; } + +.fa.fa-facebook-f { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-facebook-f:before { + content: "\f39e"; } + +.fa.fa-github { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-credit-card { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-feed:before { + content: "\f09e"; } + +.fa.fa-hdd-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hdd-o:before { + content: "\f0a0"; } + +.fa.fa-hand-o-right { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-o-right:before { + content: "\f0a4"; } + +.fa.fa-hand-o-left { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-o-left:before { + content: "\f0a5"; } + +.fa.fa-hand-o-up { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-o-up:before { + content: "\f0a6"; } + +.fa.fa-hand-o-down { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-o-down:before { + content: "\f0a7"; } + +.fa.fa-globe:before { + content: "\f57d"; } + +.fa.fa-tasks:before { + content: "\f828"; } + +.fa.fa-arrows-alt:before { + content: "\f31e"; } + +.fa.fa-group:before { + content: "\f0c0"; } + +.fa.fa-chain:before { + content: "\f0c1"; } + +.fa.fa-cut:before { + content: "\f0c4"; } + +.fa.fa-files-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-files-o:before { + content: "\f0c5"; } + +.fa.fa-floppy-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-floppy-o:before { + content: "\f0c7"; } + +.fa.fa-save { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-save:before { + content: "\f0c7"; } + +.fa.fa-navicon:before { + content: "\f0c9"; } + +.fa.fa-reorder:before { + content: "\f0c9"; } + +.fa.fa-magic:before { + content: "\e2ca"; } + +.fa.fa-pinterest { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pinterest-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pinterest-square:before { + content: "\f0d3"; } + +.fa.fa-google-plus-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-google-plus-square:before { + content: "\f0d4"; } + +.fa.fa-google-plus { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-google-plus:before { + content: "\f0d5"; } + +.fa.fa-money:before { + content: "\f3d1"; } + +.fa.fa-unsorted:before { + content: "\f0dc"; } + +.fa.fa-sort-desc:before { + content: "\f0dd"; } + +.fa.fa-sort-asc:before { + content: "\f0de"; } + +.fa.fa-linkedin { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-linkedin:before { + content: "\f0e1"; } + +.fa.fa-rotate-left:before { + content: "\f0e2"; } + +.fa.fa-legal:before { + content: "\f0e3"; } + +.fa.fa-tachometer:before { + content: "\f625"; } + +.fa.fa-dashboard:before { + content: "\f625"; } + +.fa.fa-comment-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-comment-o:before { + content: "\f075"; } + +.fa.fa-comments-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-comments-o:before { + content: "\f086"; } + +.fa.fa-flash:before { + content: "\f0e7"; } + +.fa.fa-clipboard:before { + content: "\f0ea"; } + +.fa.fa-lightbulb-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-lightbulb-o:before { + content: "\f0eb"; } + +.fa.fa-exchange:before { + content: "\f362"; } + +.fa.fa-cloud-download:before { + content: "\f0ed"; } + +.fa.fa-cloud-upload:before { + content: "\f0ee"; } + +.fa.fa-bell-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-bell-o:before { + content: "\f0f3"; } + +.fa.fa-cutlery:before { + content: "\f2e7"; } + +.fa.fa-file-text-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-text-o:before { + content: "\f15c"; } + +.fa.fa-building-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-building-o:before { + content: "\f1ad"; } + +.fa.fa-hospital-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hospital-o:before { + content: "\f0f8"; } + +.fa.fa-tablet:before { + content: "\f3fa"; } + +.fa.fa-mobile:before { + content: "\f3cd"; } + +.fa.fa-mobile-phone:before { + content: "\f3cd"; } + +.fa.fa-circle-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-circle-o:before { + content: "\f111"; } + +.fa.fa-mail-reply:before { + content: "\f3e5"; } + +.fa.fa-github-alt { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-folder-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-folder-o:before { + content: "\f07b"; } + +.fa.fa-folder-open-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-folder-open-o:before { + content: "\f07c"; } + +.fa.fa-smile-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-smile-o:before { + content: "\f118"; } + +.fa.fa-frown-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-frown-o:before { + content: "\f119"; } + +.fa.fa-meh-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-meh-o:before { + content: "\f11a"; } + +.fa.fa-keyboard-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-keyboard-o:before { + content: "\f11c"; } + +.fa.fa-flag-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-flag-o:before { + content: "\f024"; } + +.fa.fa-mail-reply-all:before { + content: "\f122"; } + +.fa.fa-star-half-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-star-half-o:before { + content: "\f5c0"; } + +.fa.fa-star-half-empty { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-star-half-empty:before { + content: "\f5c0"; } + +.fa.fa-star-half-full { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-star-half-full:before { + content: "\f5c0"; } + +.fa.fa-code-fork:before { + content: "\f126"; } + +.fa.fa-chain-broken:before { + content: "\f127"; } + +.fa.fa-unlink:before { + content: "\f127"; } + +.fa.fa-calendar-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-calendar-o:before { + content: "\f133"; } + +.fa.fa-maxcdn { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-html5 { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-css3 { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-unlock-alt:before { + content: "\f09c"; } + +.fa.fa-minus-square-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-minus-square-o:before { + content: "\f146"; } + +.fa.fa-level-up:before { + content: "\f3bf"; } + +.fa.fa-level-down:before { + content: "\f3be"; } + +.fa.fa-pencil-square:before { + content: "\f14b"; } + +.fa.fa-external-link-square:before { + content: "\f360"; } + +.fa.fa-compass { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-down { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-down:before { + content: "\f150"; } + +.fa.fa-toggle-down { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-toggle-down:before { + content: "\f150"; } + +.fa.fa-caret-square-o-up { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-up:before { + content: "\f151"; } + +.fa.fa-toggle-up { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-toggle-up:before { + content: "\f151"; } + +.fa.fa-caret-square-o-right { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-right:before { + content: "\f152"; } + +.fa.fa-toggle-right { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-toggle-right:before { + content: "\f152"; } + +.fa.fa-eur:before { + content: "\f153"; } + +.fa.fa-euro:before { + content: "\f153"; } + +.fa.fa-gbp:before { + content: "\f154"; } + +.fa.fa-usd:before { + content: "\24"; } + +.fa.fa-dollar:before { + content: "\24"; } + +.fa.fa-inr:before { + content: "\e1bc"; } + +.fa.fa-rupee:before { + content: "\e1bc"; } + +.fa.fa-jpy:before { + content: "\f157"; } + +.fa.fa-cny:before { + content: "\f157"; } + +.fa.fa-rmb:before { + content: "\f157"; } + +.fa.fa-yen:before { + content: "\f157"; } + +.fa.fa-rub:before { + content: "\f158"; } + +.fa.fa-ruble:before { + content: "\f158"; } + +.fa.fa-rouble:before { + content: "\f158"; } + +.fa.fa-krw:before { + content: "\f159"; } + +.fa.fa-won:before { + content: "\f159"; } + +.fa.fa-btc { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitcoin { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitcoin:before { + content: "\f15a"; } + +.fa.fa-file-text:before { + content: "\f15c"; } + +.fa.fa-sort-alpha-asc:before { + content: "\f15d"; } + +.fa.fa-sort-alpha-desc:before { + content: "\f881"; } + +.fa.fa-sort-amount-asc:before { + content: "\f884"; } + +.fa.fa-sort-amount-desc:before { + content: "\f160"; } + +.fa.fa-sort-numeric-asc:before { + content: "\f162"; } + +.fa.fa-sort-numeric-desc:before { + content: "\f886"; } + +.fa.fa-youtube-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-youtube-square:before { + content: "\f431"; } + +.fa.fa-youtube { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-xing { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-xing-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-xing-square:before { + content: "\f169"; } + +.fa.fa-youtube-play { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-youtube-play:before { + content: "\f167"; } + +.fa.fa-dropbox { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-stack-overflow { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-instagram { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-flickr { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-adn { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitbucket { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitbucket-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bitbucket-square:before { + content: "\f171"; } + +.fa.fa-tumblr { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-tumblr-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-tumblr-square:before { + content: "\f174"; } + +.fa.fa-long-arrow-down:before { + content: "\f309"; } + +.fa.fa-long-arrow-up:before { + content: "\f30c"; } + +.fa.fa-long-arrow-left:before { + content: "\f30a"; } + +.fa.fa-long-arrow-right:before { + content: "\f30b"; } + +.fa.fa-apple { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-windows { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-android { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-linux { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-dribbble { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-skype { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-foursquare { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-trello { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gratipay { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gittip { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gittip:before { + content: "\f184"; } + +.fa.fa-sun-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-sun-o:before { + content: "\f185"; } + +.fa.fa-moon-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-moon-o:before { + content: "\f186"; } + +.fa.fa-vk { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-weibo { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-renren { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pagelines { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-stack-exchange { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-arrow-circle-o-right { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-arrow-circle-o-right:before { + content: "\f35a"; } + +.fa.fa-arrow-circle-o-left { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-arrow-circle-o-left:before { + content: "\f359"; } + +.fa.fa-caret-square-o-left { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-caret-square-o-left:before { + content: "\f191"; } + +.fa.fa-toggle-left { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-toggle-left:before { + content: "\f191"; } + +.fa.fa-dot-circle-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-dot-circle-o:before { + content: "\f192"; } + +.fa.fa-vimeo-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-vimeo-square:before { + content: "\f194"; } + +.fa.fa-try:before { + content: "\e2bb"; } + +.fa.fa-turkish-lira:before { + content: "\e2bb"; } + +.fa.fa-plus-square-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-plus-square-o:before { + content: "\f0fe"; } + +.fa.fa-slack { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wordpress { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-openid { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-institution:before { + content: "\f19c"; } + +.fa.fa-bank:before { + content: "\f19c"; } + +.fa.fa-mortar-board:before { + content: "\f19d"; } + +.fa.fa-yahoo { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-google { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-reddit { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-reddit-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-reddit-square:before { + content: "\f1a2"; } + +.fa.fa-stumbleupon-circle { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-stumbleupon { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-delicious { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-digg { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pied-piper-pp { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pied-piper-alt { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-drupal { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-joomla { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-behance { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-behance-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-behance-square:before { + content: "\f1b5"; } + +.fa.fa-steam { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-steam-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-steam-square:before { + content: "\f1b7"; } + +.fa.fa-automobile:before { + content: "\f1b9"; } + +.fa.fa-cab:before { + content: "\f1ba"; } + +.fa.fa-spotify { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-deviantart { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-soundcloud { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-file-pdf-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-pdf-o:before { + content: "\f1c1"; } + +.fa.fa-file-word-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-word-o:before { + content: "\f1c2"; } + +.fa.fa-file-excel-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-excel-o:before { + content: "\f1c3"; } + +.fa.fa-file-powerpoint-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-powerpoint-o:before { + content: "\f1c4"; } + +.fa.fa-file-image-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-image-o:before { + content: "\f1c5"; } + +.fa.fa-file-photo-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-photo-o:before { + content: "\f1c5"; } + +.fa.fa-file-picture-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-picture-o:before { + content: "\f1c5"; } + +.fa.fa-file-archive-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-archive-o:before { + content: "\f1c6"; } + +.fa.fa-file-zip-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-zip-o:before { + content: "\f1c6"; } + +.fa.fa-file-audio-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-audio-o:before { + content: "\f1c7"; } + +.fa.fa-file-sound-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-sound-o:before { + content: "\f1c7"; } + +.fa.fa-file-video-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-video-o:before { + content: "\f1c8"; } + +.fa.fa-file-movie-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-movie-o:before { + content: "\f1c8"; } + +.fa.fa-file-code-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-file-code-o:before { + content: "\f1c9"; } + +.fa.fa-vine { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-codepen { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-jsfiddle { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-life-bouy:before { + content: "\f1cd"; } + +.fa.fa-life-buoy:before { + content: "\f1cd"; } + +.fa.fa-life-saver:before { + content: "\f1cd"; } + +.fa.fa-support:before { + content: "\f1cd"; } + +.fa.fa-circle-o-notch:before { + content: "\f1ce"; } + +.fa.fa-rebel { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-ra { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-ra:before { + content: "\f1d0"; } + +.fa.fa-resistance { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-resistance:before { + content: "\f1d0"; } + +.fa.fa-empire { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-ge { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-ge:before { + content: "\f1d1"; } + +.fa.fa-git-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-git-square:before { + content: "\f1d2"; } + +.fa.fa-git { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-hacker-news { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-y-combinator-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-y-combinator-square:before { + content: "\f1d4"; } + +.fa.fa-yc-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yc-square:before { + content: "\f1d4"; } + +.fa.fa-tencent-weibo { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-qq { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-weixin { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wechat { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wechat:before { + content: "\f1d7"; } + +.fa.fa-send:before { + content: "\f1d8"; } + +.fa.fa-paper-plane-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-paper-plane-o:before { + content: "\f1d8"; } + +.fa.fa-send-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-send-o:before { + content: "\f1d8"; } + +.fa.fa-circle-thin { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-circle-thin:before { + content: "\f111"; } + +.fa.fa-header:before { + content: "\f1dc"; } + +.fa.fa-futbol-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-futbol-o:before { + content: "\f1e3"; } + +.fa.fa-soccer-ball-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-soccer-ball-o:before { + content: "\f1e3"; } + +.fa.fa-slideshare { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-twitch { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yelp { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-newspaper-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-newspaper-o:before { + content: "\f1ea"; } + +.fa.fa-paypal { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-google-wallet { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-visa { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-mastercard { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-discover { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-amex { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-paypal { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-stripe { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bell-slash-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-bell-slash-o:before { + content: "\f1f6"; } + +.fa.fa-trash:before { + content: "\f2ed"; } + +.fa.fa-copyright { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-eyedropper:before { + content: "\f1fb"; } + +.fa.fa-area-chart:before { + content: "\f1fe"; } + +.fa.fa-pie-chart:before { + content: "\f200"; } + +.fa.fa-line-chart:before { + content: "\f201"; } + +.fa.fa-lastfm { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-lastfm-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-lastfm-square:before { + content: "\f203"; } + +.fa.fa-ioxhost { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-angellist { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-cc:before { + content: "\f20a"; } + +.fa.fa-ils:before { + content: "\f20b"; } + +.fa.fa-shekel:before { + content: "\f20b"; } + +.fa.fa-sheqel:before { + content: "\f20b"; } + +.fa.fa-buysellads { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-connectdevelop { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-dashcube { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-forumbee { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-leanpub { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-sellsy { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-shirtsinbulk { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-simplybuilt { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-skyatlas { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-diamond { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-diamond:before { + content: "\f3a5"; } + +.fa.fa-transgender:before { + content: "\f224"; } + +.fa.fa-intersex:before { + content: "\f224"; } + +.fa.fa-transgender-alt:before { + content: "\f225"; } + +.fa.fa-facebook-official { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-facebook-official:before { + content: "\f09a"; } + +.fa.fa-pinterest-p { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-whatsapp { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-hotel:before { + content: "\f236"; } + +.fa.fa-viacoin { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-medium { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-y-combinator { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yc { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yc:before { + content: "\f23b"; } + +.fa.fa-optin-monster { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-opencart { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-expeditedssl { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-battery-4:before { + content: "\f240"; } + +.fa.fa-battery:before { + content: "\f240"; } + +.fa.fa-battery-3:before { + content: "\f241"; } + +.fa.fa-battery-2:before { + content: "\f242"; } + +.fa.fa-battery-1:before { + content: "\f243"; } + +.fa.fa-battery-0:before { + content: "\f244"; } + +.fa.fa-object-group { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-object-ungroup { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-sticky-note-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-sticky-note-o:before { + content: "\f249"; } + +.fa.fa-cc-jcb { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-cc-diners-club { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-clone { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hourglass-o:before { + content: "\f254"; } + +.fa.fa-hourglass-1:before { + content: "\f251"; } + +.fa.fa-hourglass-2:before { + content: "\f252"; } + +.fa.fa-hourglass-3:before { + content: "\f253"; } + +.fa.fa-hand-rock-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-rock-o:before { + content: "\f255"; } + +.fa.fa-hand-grab-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-grab-o:before { + content: "\f255"; } + +.fa.fa-hand-paper-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-paper-o:before { + content: "\f256"; } + +.fa.fa-hand-stop-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-stop-o:before { + content: "\f256"; } + +.fa.fa-hand-scissors-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-scissors-o:before { + content: "\f257"; } + +.fa.fa-hand-lizard-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-lizard-o:before { + content: "\f258"; } + +.fa.fa-hand-spock-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-spock-o:before { + content: "\f259"; } + +.fa.fa-hand-pointer-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-pointer-o:before { + content: "\f25a"; } + +.fa.fa-hand-peace-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-hand-peace-o:before { + content: "\f25b"; } + +.fa.fa-registered { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-creative-commons { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gg { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gg-circle { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-odnoklassniki { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-odnoklassniki-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-odnoklassniki-square:before { + content: "\f264"; } + +.fa.fa-get-pocket { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wikipedia-w { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-safari { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-chrome { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-firefox { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-opera { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-internet-explorer { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-television:before { + content: "\f26c"; } + +.fa.fa-contao { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-500px { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-amazon { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-calendar-plus-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-calendar-plus-o:before { + content: "\f271"; } + +.fa.fa-calendar-minus-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-calendar-minus-o:before { + content: "\f272"; } + +.fa.fa-calendar-times-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-calendar-times-o:before { + content: "\f273"; } + +.fa.fa-calendar-check-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-calendar-check-o:before { + content: "\f274"; } + +.fa.fa-map-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-map-o:before { + content: "\f279"; } + +.fa.fa-commenting:before { + content: "\f4ad"; } + +.fa.fa-commenting-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-commenting-o:before { + content: "\f4ad"; } + +.fa.fa-houzz { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-vimeo { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-vimeo:before { + content: "\f27d"; } + +.fa.fa-black-tie { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-fonticons { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-reddit-alien { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-edge { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-credit-card-alt:before { + content: "\f09d"; } + +.fa.fa-codiepie { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-modx { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-fort-awesome { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-usb { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-product-hunt { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-mixcloud { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-scribd { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-pause-circle-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-pause-circle-o:before { + content: "\f28b"; } + +.fa.fa-stop-circle-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-stop-circle-o:before { + content: "\f28d"; } + +.fa.fa-bluetooth { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-bluetooth-b { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-gitlab { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wpbeginner { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wpforms { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-envira { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wheelchair-alt { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-wheelchair-alt:before { + content: "\f368"; } + +.fa.fa-question-circle-o { + font-family: 'Font Awesome 6 Free'; + font-weight: 400; } + +.fa.fa-question-circle-o:before { + content: "\f059"; } + +.fa.fa-volume-control-phone:before { + content: "\f2a0"; } + +.fa.fa-asl-interpreting:before { + content: "\f2a3"; } + +.fa.fa-deafness:before { + content: "\f2a4"; } + +.fa.fa-hard-of-hearing:before { + content: "\f2a4"; } + +.fa.fa-glide { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-glide-g { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-signing:before { + content: "\f2a7"; } + +.fa.fa-viadeo { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-viadeo-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-viadeo-square:before { + content: "\f2aa"; } + +.fa.fa-snapchat { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-snapchat-ghost { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-snapchat-ghost:before { + content: "\f2ab"; } + +.fa.fa-snapchat-square { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-snapchat-square:before { + content: "\f2ad"; } + +.fa.fa-pied-piper { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-first-order { + font-family: 'Font Awesome 6 Brands'; + font-weight: 400; } + +.fa.fa-yoast { + 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+
+ + + + +
+
+
+

iUSEiSEE - The iSEE package for efficient, +interactive, and reproducible exploration of data

+

Federico +Marini1, +Najla Abassi2

+
+Collection of material developed by the iSEE core dev team
(Kevin +Rue-Albrecht, Federico Marini, Charlotte Soneson, Aaron Lun)

+ + + Source: vignettes/iUSEiSEE_01.Rmd + + +
+ + + +

+
+

+iUSEiSEE: a masterclass-level workshop on iSEE +

+

The iSEE package (Rue-Albrecht et al. +2018) is a flexible, powerful and extendible application to +explore any dataset stored in SummarizedExperiment objects, including +single cell and spatially resolved data.

+

This workshop and associated workshop package contain all the +materials you might need to have a full experience and learn how to use +iSEE, hands on, starting from the basics and making your way up to the +more advanced features.

+

This vignette contains a compact version of the more extended +material of all other detailed vignettes. Feel free to use this as a +compact index for the rest!

+

In brief, you will…

+
    +
  • Install all required packages and software
    • +
  • Go get some data
    • +
  • See how easy it is to run iSEE +
    • +
  • Obtain a quick overview of the panels and the user interface
    • +
  • Get to know the more advanced features of iSEE +
    • +
  • Be able to use iSEE on your own data (and maybe even +deploy that!)
    • +
+

Ready? Let’s go!

+
+
+

Installing and loading the required packages +

+

To run the content presented in this demo, run this following chunk +in your R environment.

+
+library("BiocManager")
+BiocManager::install("imbeimainz/iUSEiSEE", dependencies = TRUE, build_vignettes = TRUE)
+

We start by loading the packages that will be used during the +demo.

+ +

All of these would be loaded if you run

+ +

You can also clone this repository (https://imbeimainz.github.io/iUSEiSEE) locally by +git clone - it makes it easy to follow along this +vignette!

+
+
+

Let’s go get us some data! +

+

We will start very much in medias res, assuming that this +might be the ideal point where you have processed your data - early or +late phase - and now want to have the BEST look at that.

+

Navigate to https://zenodo.org/records/10084595 and download the +data (it contains the three objects you might be “coming from”).
+Unpack the archive you downloaded in the datasets subfolder +of the vignette folder.

+

We included a SingleCellExperiment object on a processed +version for the PBMC3k dataset, many of you can somehow be familiar with +this.

+

Let’s load the dataset in our environment:

+
+sce_pbmc3k <- readRDS(
+  file = system.file("datasets", "sce_pbmc3k.RDS", package = "iUSEiSEE")
+)
+
+sce_pbmc3k
+#> class: SingleCellExperiment 
+#> dim: 32738 2643 
+#> metadata(0):
+#> assays(2): counts logcounts
+#> rownames(32738): MIR1302-10 FAM138A ... AC002321.2 AC002321.1
+#> rowData names(19): ENSEMBL_ID Symbol_TENx ... FDR_cluster11
+#>   FDR_cluster12
+#> colnames(2643): Cell1 Cell2 ... Cell2699 Cell2700
+#> colData names(24): Sample Barcode ... labels_ont cell_ontology_labels
+#> reducedDimNames(3): PCA TSNE UMAP
+#> mainExpName: NULL
+#> altExpNames(0):
+
+
+

As easy as saying iSEE(sce)! +

+

Who of you is investing a fair amount/lot of time in doing proper +data exploration?

+

Who of you is familiar with the beautiful CELLxGENE tool?

+

Who of you dreamed to do even more on your data?

+

I have a quiz for you - have a look at this, for example:

+

http://shiny.imbei.uni-mainz.de:3838/iSEE_Tonsils_CD4Tcells/

+

+
+Question 1 +

How many lines of code are required to realize this?

+
    +
  1. 4-5
    +
    2. +
  2. 250-300
    3. +
  3. 7500-10000
    +
    4. +
+Question 2 +

How many of these lines had to be typed by a human?

+
    +
  1. 4-5
    +
    2. +
  2. 250-300
    +
    3. +
  3. 7500-10000
    +
    4. +

So, yes, I will show you that is as easy as that:

+
+library("iSEE")
+iSEE(sce_pbmc3k)
+

Let’s run this together and see what we have got in store.

+

+ + + + + + + + +
+
+

iSEE, “tl;dr” +

+
+

Quick tour of the panels +

+

What typical visualizations people would you use to represent and +explore your datasets?

+

Each panel has some essential content:

+
    +
  • Data parameters
    • +
  • Visual parameters
    • +
  • Selection parameters
    • +
+

Let’s have a closer look at that on the Reduced Dimension Plot 1 +panel.

+

If you have no idea of what a panel can “normally” do, look for the +question mark icon, that will start a tiny tour on its +functionality.

+
+
+

Additional UI functionality +

+

iSEE also has some additional controls, grouped into the header bar, +let’s have a look at them:

+
    +
  • Organization buttons
    • +
  • Export buttons
    • +
  • Documentation buttons
    • +
  • Extra info buttons
    • +
+

With this, we can…

+
    +
  • reorganize the panel layout - add, remove, re-order
    • +
  • extract the code to exactly reproduce the outputs, 1:1
    • +
  • store (and re-utilize) the panel settings
    • +
  • generate at once an archive with all/selected parts of the panel +outputs
    • +
  • start a tour on the content displayed - yes, you can very much tell +a story with iSEE!
    • +
+
+
+

In depth exploration of data - iSEE’s main functionality +

+
+

You can control pretty much everything in a plot +

+

Coloring by, faceting, zooming, hovering!
+Make a wish!

+
+
+ +

Brush selection, lasso selection, select the receiving panel, +iterate!
+Read out information, see the panel transmission graph.

+
+
+

You can use tables to navigate panels +

+

Search and select, transmit, receive, iterate!

+
+
+

Configuring your iSEE instance +

+

Panels: add, move, remove; exporting the initial +configuration, reloading it

+
+
+

Reproducibility for the win! +

+

A magic wand: two clicks away from all the R code you need; export +all outputs, save individual images

+
+
+

Sharing is caring, and so is telling a story +

+

Start the tour, highlighting elements; make your data really +explorable!

+
+
+
+

Custom panels & the iSEE universe +

+

A couple of additional things you can do with custom panels (and this +is just an appetizer!):

+ +
+
+

Got data? +

+
+

Got single cell data? +

+

You might have got the gist:

+
+sce <- sce_pbmc3k # Load your data in some manner
+library("iSEE")
+iSEE(sce)
+

So, yes, we mighe be down to THREE lines of code.

+
+
+

Got some special feature in your data? +

+

Maybe iSEEu can +be the place to find already that piece of functionality!

+
+
+

Got bulk? +

+

Have a look at the functionality provided by

+ +

These can be ideal companions for exploring DE results.

+

iSEEde and +iSEEpathways +are two new Bioconductor packages that provide iSEE panels +specifically aimed towards exploration of differential expression and +pathway analysis results. More precisely, iSEEde +provides the VolcanoPlot, MAPlot, +LogFCLogFCPlot and DETable panels.

+
+
+

Got many datasets? +

+

Have a look at the functionality provided by

+ +

The iSEEhub +package provides a custom landing page for an iSEE +application interfacing with the Bioconductor ExperimentHub. +The landing page allows users to browse the ExperimentHub, select a data +set, download and cache it, and import it directly into an iSEE app.

+

iSEEindex +provides an interface to any collection of data sets +within a single iSEE web-application.
+The main functionality of this package is to define a custom landing +page allowing app maintainers to list a custom collection of data sets +that users can select from and directly load objects into an iSEE web +application.

+
+

One iSEE instance to rule exlore them all…

+
+

A more elaborate example is available at https://rehwinkellab.shinyapps.io/ifnresource/. +The source can be found at https://github.com/kevinrue/IFNresource.

+ + + + + +

Potential use cases can include:

+
    +
  • An app to present and explore the different datasets in your next +publication
    • +
  • An app to explore collection of datasets collaboratively, in +consortium-like initiatives
    • +
  • An app to mirror and enhance the content of e.g. the CellxGene data +portal
    • +
  • Got any ideas on how to use iSEE for such deployments?
    • +
+
+
+
+

Interoperability +

+

“Wait, I got Seurat/Anndata objects”.

+

The single cell communities got you covered:

+
+

From Seurat… +

+
+library("Seurat")
+
+seurat_object <- readRDS("datasets/seurat_pbmc3k.RDS")
+sce_from_seurat <- Seurat::as.SingleCellExperiment(
+  seurat_object
+)
+sce_from_seurat
+
+
+iSEE(sce_from_seurat)
+
+
+

From AnnData… +

+

… which is basically able to serve any dataset available throught the +CELLxGENE Data Portal!

+
+library("zellkonverter")
+
+sce_from_anndata <- zellkonverter::readH5AD(file = "datasets/anndata_pbmc3k.h5ad")
+sce_from_anndata
+
+iSEE(sce_from_anndata)
+

An example could be…

+

https://cellxgene.cziscience.com/e/c7856243-c59a-4b70-8ce7-25b94c2d9da1.cxg/

+
+
+
+

I want moaaaaar on iSEE! +

+

For more extensive descriptions of the different parts of the +interface, we refer to the overview +vignette in this workshop material package on iSEE.

+

Additional material can also be found at these other +repositories:

+ +
+
+
+

Session info +

+
+sessionInfo()
+#> R version 4.4.0 (2024-04-24)
+#> Platform: x86_64-apple-darwin20
+#> Running under: macOS Monterey 12.7.1
+#> 
+#> Matrix products: default
+#> BLAS:   /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRblas.0.dylib 
+#> LAPACK: /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRlapack.dylib;  LAPACK version 3.12.0
+#> 
+#> locale:
+#> [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
+#> 
+#> time zone: Europe/Berlin
+#> tzcode source: internal
+#> 
+#> attached base packages:
+#> [1] stats4    stats     graphics  grDevices utils     datasets  methods  
+#> [8] base     
+#> 
+#> other attached packages:
+#>  [1] iUSEiSEE_1.0.0              iSEEindex_1.2.0            
+#>  [3] iSEEhub_1.6.0               ExperimentHub_2.12.0       
+#>  [5] AnnotationHub_3.12.0        BiocFileCache_2.12.0       
+#>  [7] dbplyr_2.5.0                iSEEpathways_1.2.0         
+#>  [9] iSEEde_1.2.0                iSEEu_1.15.1               
+#> [11] iSEEhex_1.5.0               TENxPBMCData_1.21.0        
+#> [13] HDF5Array_1.32.0            rhdf5_2.48.0               
+#> [15] DelayedArray_0.30.0         SparseArray_1.4.0          
+#> [17] S4Arrays_1.4.0              abind_1.4-5                
+#> [19] Matrix_1.7-0                iSEE_2.15.1                
+#> [21] SingleCellExperiment_1.26.0 SummarizedExperiment_1.33.3
+#> [23] Biobase_2.64.0              GenomicRanges_1.55.4       
+#> [25] GenomeInfoDb_1.40.0         IRanges_2.38.0             
+#> [27] S4Vectors_0.42.0            BiocGenerics_0.50.0        
+#> [29] MatrixGenerics_1.16.0       matrixStats_1.3.0          
+#> [31] BiocStyle_2.32.0           
+#> 
+#> loaded via a namespace (and not attached):
+#>   [1] splines_4.4.0             later_1.3.2              
+#>   [3] bitops_1.0-7              urltools_1.7.3           
+#>   [5] filelock_1.0.3            tibble_3.2.1             
+#>   [7] triebeard_0.4.1           lifecycle_1.0.4          
+#>   [9] edgeR_4.2.0               doParallel_1.0.17        
+#>  [11] lattice_0.22-6            magrittr_2.0.3           
+#>  [13] limma_3.60.0              sass_0.4.9               
+#>  [15] rmarkdown_2.26            jquerylib_0.1.4          
+#>  [17] yaml_2.3.8                httpuv_1.6.15            
+#>  [19] DBI_1.2.2                 RColorBrewer_1.1-3       
+#>  [21] zlibbioc_1.50.0           purrr_1.0.2              
+#>  [23] RCurl_1.98-1.14           rappdirs_0.3.3           
+#>  [25] circlize_0.4.16           GenomeInfoDbData_1.2.12  
+#>  [27] ggrepel_0.9.5             irlba_2.3.5.1            
+#>  [29] pkgdown_2.0.9             DelayedMatrixStats_1.26.0
+#>  [31] codetools_0.2-20          DT_0.33                  
+#>  [33] scuttle_1.14.0            tidyselect_1.2.1         
+#>  [35] shape_1.4.6.1             UCSC.utils_1.0.0         
+#>  [37] viridis_0.6.5             ScaledMatrix_1.12.0      
+#>  [39] shinyWidgets_0.8.6        jsonlite_1.8.8           
+#>  [41] GetoptLong_1.0.5          BiocNeighbors_1.22.0     
+#>  [43] scater_1.32.0             iterators_1.0.14         
+#>  [45] systemfonts_1.0.6         foreach_1.5.2            
+#>  [47] tools_4.4.0               ragg_1.3.0               
+#>  [49] Rcpp_1.0.12               glue_1.7.0               
+#>  [51] gridExtra_2.3             xfun_0.43                
+#>  [53] mgcv_1.9-1                DESeq2_1.44.0            
+#>  [55] dplyr_1.1.4               shinydashboard_0.7.2     
+#>  [57] BiocManager_1.30.22       fastmap_1.1.1            
+#>  [59] rhdf5filters_1.16.0       fansi_1.0.6              
+#>  [61] shinyjs_2.1.0             digest_0.6.35            
+#>  [63] rsvd_1.0.5                R6_2.5.1                 
+#>  [65] mime_0.12                 textshaping_0.3.7        
+#>  [67] colorspace_2.1-0          listviewer_4.0.0         
+#>  [69] RSQLite_2.3.6             paws.storage_0.5.0       
+#>  [71] utf8_1.2.4                generics_0.1.3           
+#>  [73] hexbin_1.28.3             httr_1.4.7               
+#>  [75] htmlwidgets_1.6.4         pkgconfig_2.0.3          
+#>  [77] gtable_0.3.5              blob_1.2.4               
+#>  [79] ComplexHeatmap_2.20.0     XVector_0.44.0           
+#>  [81] htmltools_0.5.8.1         rintrojs_0.3.4           
+#>  [83] clue_0.3-65               scales_1.3.0             
+#>  [85] png_0.1-8                 knitr_1.46               
+#>  [87] rstudioapi_0.16.0         rjson_0.2.21             
+#>  [89] nlme_3.1-164              curl_5.2.1               
+#>  [91] shinyAce_0.4.2            cachem_1.0.8             
+#>  [93] GlobalOptions_0.1.2       stringr_1.5.1            
+#>  [95] BiocVersion_3.19.1        parallel_4.4.0           
+#>  [97] miniUI_0.1.1.1            vipor_0.4.7              
+#>  [99] AnnotationDbi_1.65.2      desc_1.4.3               
+#> [101] pillar_1.9.0              grid_4.4.0               
+#> [103] vctrs_0.6.5               promises_1.3.0           
+#> [105] BiocSingular_1.20.0       beachmat_2.20.0          
+#> [107] xtable_1.8-4              cluster_2.1.6            
+#> [109] beeswarm_0.4.0            evaluate_0.23            
+#> [111] cli_3.6.2                 locfit_1.5-9.9           
+#> [113] compiler_4.4.0            rlang_1.1.3              
+#> [115] crayon_1.5.2              paws.common_0.7.2        
+#> [117] fs_1.6.4                  ggbeeswarm_0.7.2         
+#> [119] stringi_1.8.3             viridisLite_0.4.2        
+#> [121] BiocParallel_1.38.0       munsell_0.5.1            
+#> [123] Biostrings_2.72.0         colourpicker_1.3.0       
+#> [125] sparseMatrixStats_1.16.0  bit64_4.0.5              
+#> [127] ggplot2_3.5.1             Rhdf5lib_1.26.0          
+#> [129] KEGGREST_1.44.0           statmod_1.5.0            
+#> [131] shiny_1.8.1.1             highr_0.10               
+#> [133] igraph_2.0.3              memoise_2.0.1            
+#> [135] bslib_0.7.0               bit_4.0.5
+
+
+Rue-Albrecht, Kevin, Federico Marini, Charlotte Soneson, and Aaron T L +Lun. 2018. “iSEE: Interactive +SummarizedExperiment Explorer.” F1000Res. 7 +(June). +
+
+
+
+
+
    +

  1. ↩︎

    2. +

  2. ↩︎

    3. +
+
+
+ + + +
+ + + + +
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articles/images/ss_Tcells.png diff --git a/articles/index.html b/articles/index.html new file mode 100644 index 0000000..44f22e3 --- /dev/null +++ b/articles/index.html @@ -0,0 +1,103 @@ + +Articles • iUSEiSEE + + +
+
+ + + +
+
+
+

Articles

+
+ +
+

All vignettes

+

+ +
iUSEiSEE - About this workshop
+
+
iUSEiSEE - Bonus content
+
+
iUSEiSEE - An overview of iSEE
+
+
iUSEiSEE - The iSEE cookbook
+
+
iUSEiSEE - The iSEE package for efficient, interactive, and reproducible exploration of data
+
+
+
+
+ + +
+ + + + + + + + diff --git a/authors.html b/authors.html new file mode 100644 index 0000000..378186d --- /dev/null +++ b/authors.html @@ -0,0 +1,133 @@ + +Authors and Citation • iUSEiSEE + + +
+
+ + + +
+
+
+
+

Authors and Citation

+
+ + +
  • +

    Federico Marini. Author, maintainer. +

    +
    • +
  • +

    Kevin Rue-Albrecht. Author. +

    +
    • +
  • +

    Charlotte Soneson. Author. +

    +
    • +
  • +

    Aaron Lun. Author. +

    +
    • +
  • +

    Najla Abassi. Author. +

    +
    • +
+
+
+

Citation

+ Source: DESCRIPTION +
+
+ + +

Marini F, Rue-Albrecht K, Soneson C, Lun A, Abassi N (2024). +iUSEiSEE: A Workshop to learn how to use the iSEE package. +R package version 1.0.0, https://imbeimainz.github.io/iUSEiSEE/. +

+ 
@Manual{,
+  title = {iUSEiSEE: A Workshop to learn how to use the iSEE package},
+  author = {Federico Marini and Kevin Rue-Albrecht and Charlotte Soneson and Aaron Lun and Najla Abassi},
+  year = {2024},
+  note = {R package version 1.0.0},
+  url = {https://imbeimainz.github.io/iUSEiSEE/},
+}
+ +
+ +
+ + + +
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+ padding-top: 0 +} + +.algolia-autocomplete .algolia-docsearch-suggestion--subcategory-column { + float: none; + width: auto; + padding: 0; + text-align: left +} + +.algolia-autocomplete .algolia-docsearch-suggestion--content { + float: none; + width: auto; + padding: 0 +} + +.algolia-autocomplete .algolia-docsearch-suggestion--content::before { + display: none +} + +.algolia-autocomplete .ds-suggestion:not(:first-child) .algolia-docsearch-suggestion--category-header { + padding-top: .75rem; + margin-top: .75rem; + border-top: 1px solid rgba(0, 0, 0, .1) +} + +.algolia-autocomplete .ds-suggestion .algolia-docsearch-suggestion--subcategory-column { + display: block; + padding: .1rem 1rem; + margin-bottom: 0.1; + font-size: 1.0em; + font-weight: 400 + /* display: none */ +} + +.algolia-autocomplete .algolia-docsearch-suggestion--title { + display: block; + padding: .25rem 1rem; + margin-bottom: 0; + font-size: 0.9em; + font-weight: 400 +} + +.algolia-autocomplete .algolia-docsearch-suggestion--text { + padding: 0 1rem .5rem; + margin-top: -.25rem; + font-size: 0.8em; + font-weight: 400; + line-height: 1.25 +} + +.algolia-autocomplete .algolia-docsearch-footer { + width: 110px; + height: 20px; + z-index: 3; + margin-top: 10.66667px; + float: right; + font-size: 0; + line-height: 0; +} + +.algolia-autocomplete .algolia-docsearch-footer--logo { + background-image: url("data:image/svg+xml;utf8,"); + background-repeat: no-repeat; + background-position: 50%; + background-size: 100%; + overflow: hidden; + text-indent: -9000px; + width: 100%; + height: 100%; + display: block; + transform: translate(-8px); +} + +.algolia-autocomplete .algolia-docsearch-suggestion--highlight { + color: #FF8C00; + background: rgba(232, 189, 54, 0.1) +} + + +.algolia-autocomplete .algolia-docsearch-suggestion--text .algolia-docsearch-suggestion--highlight { + box-shadow: inset 0 -2px 0 0 rgba(105, 105, 105, .5) +} + +.algolia-autocomplete .ds-suggestion.ds-cursor .algolia-docsearch-suggestion--content { + background-color: rgba(192, 192, 192, .15) +} diff --git a/docsearch.js b/docsearch.js new file mode 100644 index 0000000..b35504c --- /dev/null +++ b/docsearch.js @@ -0,0 +1,85 @@ +$(function() { + + // register a handler to move the focus to the search bar + // upon pressing shift + "/" (i.e. "?") + $(document).on('keydown', function(e) { + if (e.shiftKey && e.keyCode == 191) { + e.preventDefault(); + $("#search-input").focus(); + } + }); + + $(document).ready(function() { + // do keyword highlighting + /* modified from https://jsfiddle.net/julmot/bL6bb5oo/ */ + var mark = function() { + + var referrer = document.URL ; + var paramKey = "q" ; + + if (referrer.indexOf("?") !== -1) { + var qs = referrer.substr(referrer.indexOf('?') + 1); + var qs_noanchor = qs.split('#')[0]; + var qsa = qs_noanchor.split('&'); + var keyword = ""; + + for (var i = 0; i < qsa.length; i++) { + var currentParam = qsa[i].split('='); + + if (currentParam.length !== 2) { + continue; + } + + if (currentParam[0] == paramKey) { + keyword = decodeURIComponent(currentParam[1].replace(/\+/g, "%20")); + } + } + + if (keyword !== "") { + $(".contents").unmark({ + done: function() { + $(".contents").mark(keyword); + } + }); + } + } + }; + + mark(); + }); +}); + +/* Search term highlighting ------------------------------*/ + +function matchedWords(hit) { + var words = []; + + var hierarchy = hit._highlightResult.hierarchy; + // loop to fetch from lvl0, lvl1, etc. + for (var idx in hierarchy) { + words = words.concat(hierarchy[idx].matchedWords); + } + + var content = hit._highlightResult.content; + if (content) { + words = words.concat(content.matchedWords); + } + + // return unique words + var words_uniq = [...new Set(words)]; + return words_uniq; +} + +function updateHitURL(hit) { + + var words = matchedWords(hit); + var url = ""; + + if (hit.anchor) { + url = hit.url_without_anchor + '?q=' + escape(words.join(" ")) + '#' + hit.anchor; + } else { + url = hit.url + '?q=' + escape(words.join(" ")); + } + + return url; +} diff --git a/pkgdown/extra.css b/extra.css similarity index 100% rename from pkgdown/extra.css rename to extra.css diff --git a/pkgdown/favicon/favicon-16x16.png b/favicon-16x16.png similarity index 100% rename from pkgdown/favicon/favicon-16x16.png rename to favicon-16x16.png diff --git a/pkgdown/favicon/favicon-32x32.png b/favicon-32x32.png similarity index 100% rename from pkgdown/favicon/favicon-32x32.png rename to favicon-32x32.png diff --git a/pkgdown/favicon/favicon.ico b/favicon.ico similarity index 100% rename from pkgdown/favicon/favicon.ico rename to favicon.ico diff --git a/iUSEiSEE.Rproj b/iUSEiSEE.Rproj deleted file mode 100644 index 88ff2b5..0000000 --- a/iUSEiSEE.Rproj +++ /dev/null @@ -1,21 +0,0 @@ -Version: 1.0 - -RestoreWorkspace: Default -SaveWorkspace: Default -AlwaysSaveHistory: Default - -EnableCodeIndexing: Yes -UseSpacesForTab: Yes -NumSpacesForTab: 2 -Encoding: UTF-8 - -RnwWeave: knitr -LaTeX: pdfLaTeX - -AutoAppendNewline: Yes -StripTrailingWhitespace: Yes - -BuildType: Package -PackageUseDevtools: Yes -PackageInstallArgs: --no-multiarch --with-keep.source -PackageRoxygenize: rd,collate,namespace diff --git a/index.html b/index.html new file mode 100644 index 0000000..199bb42 --- /dev/null +++ b/index.html @@ -0,0 +1,202 @@ + + + + + + + +iUSEiSEE • iUSEiSEE + + + + + + + + + + + + + + + + + + + +
    +
    + + + + +
    +
    + +

    +
    +

    iUSEiSEE +

    +

    The iSEE package is a flexible, powerful and extendible application to explore any dataset stored in SummarizedExperiment objects, including single cell and spatially resolved data.

    +

    iSEE enables a multitude of panels to gain in-depth insight in your data; it does so combining interactivity and reproducibility, and providing an ideal companion to CELLxGENE for deeper dives into data.

    +
    +

    How to install +

    +
    library("remotes")
+remotes::install_github("imbeimainz/iUSEiSEE", 
+                        dependencies = TRUE, 
+                        build_vignettes = TRUE)
    +
    +
    +

    How to run this workshop +

    +
      +
    • Open the iUSEiSEE.Rproj project file
      • +
    • Navigate to the vignettes folder
      • +
    • Make sure the datasets are included in the datasets subfolder (if using Seurat or AnnData)
      • +
    • Open the individual Rmarkdown files and execute their content live!
      • +
    +
    +
    +

    Schedule +

    +
      +
    • Short presentation, iSEE: Harry met Sally for interactive and reproducible data exploration
      • +
    • Installation of the dependencies & tech check
      • +
    • Retrieval of the data + Inspecting the processing steps so far
      • +
    • Overview on iSEE: panel by panel, learning Data + Visual + Selection parameters
      • +
    • Hands on with the full workshop
      • +
    • Going through the iSEE cookbook: “What if all I need is some recipes?”
      • +
    • Latest developments in the iSEE universe: iSEE for DE and pathways
      • +
    • Got more than just one dataset? iSEEhub and iSEEindex
      • +
    • iSEEfier: starting things up in a breeze
      • +
    • Got some own data? Some space for additional questions and setting up iSEE on that
      • +
    +
    +
    + +
    + + +
    + + +
    +

    +

    Developed by Federico Marini, Kevin Rue-Albrecht, Charlotte Soneson, Aaron Lun, Najla Abassi.

    +
    + +
    +

    +

    Site built with pkgdown 2.0.9.

    +
    + +
    +
    + + + + + + + + diff --git a/inst/create_datasets.Rmd b/inst/create_datasets.Rmd deleted file mode 100644 index e7fdfe1..0000000 --- a/inst/create_datasets.Rmd +++ /dev/null @@ -1,527 +0,0 @@ -# Working with the `pbmc3k` dataset - -## Preparation of example scRNAseq data - -```{r libbioc} -library("BiocStyle") -``` - - -In this workshop, we use example data from the `r Biocpkg("TENxPBMCData")` package. -This package provides an _R / Bioconductor_ resource for representing and manipulating different single-cell RNA-seq data sets profiling peripheral blood mononuclear cells (PBMC) generated by 10x Genomics (https://support.10xgenomics.com/single-cell-gene-expression/datasets). - -```{r loadtenx, message=FALSE} -library("TENxPBMCData") -``` - -The man page for the `TENxPBMCData()` function gives an idea of the datasets that are available from this package. -It can be opened with the following command. - -```{r helptenx, eval=FALSE} -help(TENxPBMCData) -``` - -Here, we use the `pbmc3k` dataset, which contains gene expression profiles for 2,700 single peripheral blood mononuclear cells. -The first time this dataset is loaded, this command downloads the dataset to a local cache, which takes some time, depending on the speed of your internet connection. -Subsequent times, the same command loads the dataset directly from the local cache. - -```{r loadsce, message=FALSE, warning=FALSE} -sce <- TENxPBMCData(dataset = "pbmc3k") -``` - -At this point we can inspect the dataset in the console. - -```{r printsce} -sce -``` - -```{r} -counts(sce) <- as(counts(sce), "dgCMatrix") -``` - - -The dataset is provided as an object of the `SingleCellExperiment` class. -In particular, this summary view indicates that the following pieces of information are available: - -- An assay matrix named `counts` -- Row (i.e., gene) names are Ensembl gene IDs -- Row metadata include for each gene the official gene symbol, and the gene symbol used by the -10x CellRanger quantification pipeline -- Column (i.e., cell) IDs are not initialized and left to `NULL` -- Column metadata include diverse information for each cell, including the cell barcode (`Barcode`) and the donor identifier (`Individual`). - -Note that a `SingleCellExperiment` object (or, more generally, any `SummarizedExperiment` object) like this one already contains sufficient information to launch an interactive application instance to visualize the available data and metadata, using the `iSEE()` function. - -For the purpose of this workshop, we first apply some preprocessing to the `SingleCellExperiment` object, in order to populate it with more information that can be visualized with `iSEE`. - -We start by adding column names to the object, and use gene symbols instead of Ensembl IDs as row names. -In the case where multiple Ensembl identifiers correspond to the same gene symbol, the `scater::uniquifyFeatureNames` function concatenates the Ensembl ID and the gene symbol in order to generate unique feature names. - -```{r rownamesrename, message=FALSE} -library("scater") -colnames(sce) <- paste0("Cell", seq_len(ncol(sce))) -rownames(sce) <- scater::uniquifyFeatureNames( - ID = rowData(sce)$ENSEMBL_ID, - names = rowData(sce)$Symbol_TENx -) -head(rownames(sce)) -``` - -Next, we use the `r Biocpkg("scater")` package to calculate gene- and cell-level quality metrics. -These metrics are added as columns to the `rowData` and `colData` slots of the `SingleCellExperiment` object, respectively. -We also add some additional metrics that are not automatically computed by the `r Biocpkg("scater")` package. - -```{r mitos, message = FALSE} -MT <- rownames(sce)[grep("^MT-", rownames(sce))] -sce <- scater::addPerCellQC(sce, subsets = list(MT = MT)) -sce <- scater::addPerFeatureQC(sce) -sce$log10_total <- log10(sce$total) -rowData(sce)$n_cells <- as.integer(rowData(sce)$detected * ncol(sce)) -rowData(sce)$log10_total <- log10(rowSums(assay(sce, "counts")) + 1) -sce -``` - -We filter out a few cells with a large fraction of the counts coming from mitochondrial genes, since these may be damaged cells. -Notice the reduced number of columns in the dataset below. - -```{r mitofilter} -(sce <- sce[, sce$subsets_MT_percent < 5]) -``` - -Next, we calculate size factors and normalized and log-transformed expression values, using the `r Biocpkg("scran")` and `r Biocpkg("scater")` packages. -Note that it is typically recommended to pre-cluster the cells before computing the size factors, as follows: - - - - - - - - - - - -```{r normalize, message=FALSE} -library("scran") -set.seed(1000) -sce <- scran::computeSumFactors(sce, min.mean = 0.1) -summary(sizeFactors(sce)) -sce <- scater::logNormCounts(sce) -``` - -In order to extract the most informative genes, we first model the mean-variance trend and decompose the variance into biological and technical components. - -```{r mgv} -dec <- scran::modelGeneVar(sce) -top.dec <- dec[order(dec$bio, decreasing = TRUE), ] -head(top.dec) -``` - -Next, we apply Principal Components Analysis (PCA) and t-distributed Stochastic Neighbor Embedding (t-SNE) to generate low-dimensional representations of the cells in our data set. -These low-dimensional representations are added to the `reducedDim` slot of the `SingleCellExperiment` object. - -```{r reddims, message=FALSE} -library("BiocSingular") -set.seed(1000) -sce <- scran::denoisePCA(sce, technical = dec) -ncol(reducedDim(sce, "PCA")) -set.seed(1000) -sce <- scater::runTSNE(sce, dimred = "PCA", perplexity = 30) -sce <- scater::runUMAP(sce, dimred = "PCA") -sce -``` - -Finally, we cluster the cells using a graph-based algorithm, and find 'marker genes' for each cluster as the genes that are significantly upregulated in the cluster compared to each of the other inferred clusters. -The adjusted p-values from this test, for each cluster, are added to the `rowData` slot of the object. - -```{r clusters} -snn.gr <- scran::buildSNNGraph(sce, use.dimred = "PCA") -clusters <- igraph::cluster_walktrap(snn.gr) -sce$Cluster <- factor(clusters$membership) -table(sce$Cluster) - -markers <- scran::findMarkers(sce, - groups = sce$Cluster, - test.type = "t", - direction = "up", pval.type = "all" -) -for (i in names(markers)) { - rowData(sce)[, paste0("FDR_cluster", i)] <- - markers[[i]]$FDR[match( - rownames(sce), - rownames(markers[[i]]) - )] -} -sce -``` - - -Finally, we assign a label to each cell, based on their individual transcriptome profiles, using the `SingleR` method and the Monaco immune data (https://doi.org/10.1016/j.celrep.2019.01.041) as a reference. -For each prediction, we assign the labels values to a specific `colData` element - -```{r loadsingler, message=FALSE} -library("SingleR") -library("celldex") -ref_monaco <- MonacoImmuneData() -``` - -Here we assign the cell type according to the main classification scheme -(this includes `r knitr::combine_words(names(table(ref_monaco$label.main)))`) - -```{r annomain} -pred_monaco_main <- SingleR( - test = sce, - ref = ref_monaco, - labels = ref_monaco$label.main -) -table(pred_monaco_main$labels) -sce$labels_main <- pred_monaco_main$labels -``` - -We do something similar with a more fine-grained classification, this time including the cell subtypes (e.g., for `r sort(unique(ref_monaco$label.main))[1]`, the subtypes would include `r knitr::combine_words(names(table((ref_monaco$label.main), (ref_monaco$label.fine))[1, ])[which(table((ref_monaco$label.main), (ref_monaco$label.fine))[1,] > 0)])`) - -```{r annofine} -pred_monaco_fine <- SingleR( - test = sce, - ref = ref_monaco, - labels = ref_monaco$label.fine -) -table(pred_monaco_fine$labels) -sce$labels_fine <- pred_monaco_fine$labels -``` - -Similarly, we use the information contained in the cell ontology labels. - -```{r annocl} -pred_monaco_ont <- SingleR( - test = sce, - ref = ref_monaco, - labels = ref_monaco$label.ont -) -table(pred_monaco_ont$labels) -sce$labels_ont <- pred_monaco_ont$labels -``` - -We can add that info in a more readable way with this set of commands - -```{r infocellonto} -library("ontoProc") -cl <- getOnto("cellOnto") -cl - -translated <- cl$name[ref_monaco$label.ont] -head(translated) - -sce$cell_ontology_labels <- cl$name[pred_monaco_ont$labels] -``` - - -The next table shows the relationship between the coarse and fine grained assignments in the data at hand. - -```{r compareanno} -table( - sce$labels_main, - sce$labels_fine -) - -table( - sce$labels_main, - sce$cell_ontology_labels -) -``` - - -This concludes the preparation of the data. -We have now a `SingleCellExperiment` object that contains different types of abundance values, representations in reduced dimensions, as well as a range of row (feature) and column (cell) metadata. - -We can launch an `r Biocpkg("iSEE")` instance for exploring this data set using the `iSEE()` function: - -```{r launchisee, message=FALSE} -library("iSEE") - -if (interactive()) { - iSEE(sce) -} -``` - -```{r saveobj} -saveRDS(sce, - file = "datasets/sce_pbmc3k.RDS" -) - -sce_pbmc3k <- readRDS("datasets/sce_pbmc3k.RDS") -``` - - -## Interoperability - -### What about the same dataset, but for Seurat? - -```{r convertseurat, eval=FALSE} -library("Seurat") -# seurat_object <- as.Seurat(sce) - -seurat_object <- Seurat::CreateSeuratObject( - counts = as.matrix(counts(sce)), - meta.data = as.data.frame(colData(sce)) -) - -seurat_object <- Seurat::SetAssayData( - object = seurat_object, - slot = "data", - new.data = as.matrix(logcounts(sce)) -) - -# Set feature metadata, AKA rowData. Super intuitive, right? -seurat_object[["RNA"]][[]] <- as.data.frame(rowData(sce)) - -seurat_object <- FindVariableFeatures(seurat_object, selection.method = "vst", nfeatures = 2000) - -all.genes <- rownames(seurat_object) -seurat_object <- ScaleData(seurat_object, features = all.genes) - -seurat_object <- RunPCA(seurat_object, features = VariableFeatures(seurat_object)) -seurat_object <- RunUMAP(seurat_object, dims = 1:10) - -saveRDS(seurat_object, - file = "datasets/seurat_pbmc3k.RDS" -) - -sce_from_seurat <- Seurat::as.SingleCellExperiment( - readRDS("datasets/seurat_pbmc3k.RDS") -) -sce_from_seurat - -if (interactive()) { - iSEE(sce_from_seurat) -} -``` - -### And where is my AnnData object? - -```{r convertanndata} -library("zellkonverter") - -sce$Tissue_status <- NULL - -zellkonverter::writeH5AD(sce, - file = "datasets/anndata_pbmc3k.h5ad", - compression = "gzip" -) - -sce_from_anndata <- zellkonverter::readH5AD(file = "datasets/anndata_pbmc3k.h5ad") -sce_from_anndata - -if (interactive()) { - iSEE(sce_from_anndata) -} -``` - - -# Another single-cell dataset, from Bartneck et al. - -To obtain a preprocessed version of this dataset, navigate to - -https://seafile.rlp.net/d/c7f1335a6248427d97da/ - -then navigate to the `sce_objects` folder, and from there select the `sce_merged_allsets.RDS` file, and download it locally. - - -# `iSEE` bulk data! Loading and processing the `macrophage` data set - -The data set that will be used for this demo is a bulk RNA-seq data set containing 24 samples from Alasoo, et al: "Shared genetic effects on chromatin and gene expression indicate a role for enhancer priming in immune response", published in Nature Genetics, January 2018. -The 24 samples correspond to 6 different donors. -For each of these we have four samples: one naive, one exposed to IFNgamma, one exposed to Salmonella, and one exposed to IFNgamma and Salmonella. -The `r Biocpkg("macrophage")` Bioconductor package provides the output files resulting from running Salmon on these 24 samples. -Here, we load the quantifications into R using the `r Biocpkg("tximeta")` package. - -```{r macrophagerun} -library("macrophage") -library("DESeq2") -library("limma") -library("edgeR") -library("tximeta") -library("org.Hs.eg.db") -library("fgsea") -library("GO.db") -library("stringr") -library("SingleCellExperiment") - -library("iSEEde") -library("iSEEpathways") - -## Set the data directory -dir <- system.file("extdata", package = "macrophage") - -## Read sample annotations -coldata <- read.csv(file.path(dir, "coldata.csv"))[, c(1, 2, 3, 5)] - -## Create new columns indicating, respectively whether the sample was exposed -## to IFNgamma and Salmonella -coldata$IFNg <- as.character(grepl("IFNg", coldata$condition_name)) -coldata$SL1344 <- as.character(grepl("SL1344", coldata$condition_name)) - -## Add paths to quantification files and import data -coldata$files <- file.path(dir, "quants", coldata$names, "quant.sf.gz") -se <- tximeta(coldata = coldata, type = "salmon", dropInfReps = TRUE) - -## Summarize to the gene level and add additional identifiers -seg <- summarizeToGene(se) -rownames(seg) <- str_replace(rownames(seg), "\\.\\d+$", "") -seg <- addIds(seg, "SYMBOL") -seg <- addIds(seg, "GOALL", multiVals = "list") -rownames(seg) <- scater::uniquifyFeatureNames( - ID = rownames(seg), names = rowData(seg)$SYMBOL -) - -## Create a DESeqDataSet and filter lowly expressed genes -dds <- DESeqDataSet(seg, design = ~ IFNg * SL1344) -keep <- rowSums(counts(dds)) >= 10 -dds <- dds[keep, ] - -dds - -## Apply a variance stabilizing transform followed by PCA -vst <- DESeq2::varianceStabilizingTransformation(dds, blind = TRUE) -pca <- DESeq2::plotPCA(vst, intgroup = "condition_name", returnData = TRUE) - -## Create a SingleCellExperiment object and include the PCA representation -sce_macrophage <- as(dds, "SingleCellExperiment") -assay(sce_macrophage, "vst") <- assay(vst) -SingleCellExperiment::reducedDim(sce_macrophage, "PCA") <- pca[, c("PC1", "PC2")] - -sce_macrophage -``` - -## Performing differential expression analysis - -Below, we will illustrate how to use the `r Biocpkg("iSEEde")` and `r Biocpkg("iSEEpathways")` packages to visualize results from differential expression analyses. -Thus, we first apply the `r Biocpkg("DESeq2")` and `r Biocpkg("limma")` packages to perform differential expression analysis. -We test the interaction effect between IFNgamma and Salmonella exposure, to investigate whether the effect of Salmonella exposure is different depending on whether or not the sample was also exposed to IFNgamma. - -```{r runlimma} -## Run DESeq2 -dds <- DESeq2::DESeq(dds) -DESeq2::resultsNames(dds) -res <- DESeq2::results(dds, - name = "IFNgTRUE.SL1344TRUE", - lfcThreshold = 0 -) - -## Embed the contrast results obtained from DESeq2 into the SCE -stopifnot(rownames(res) == rownames(sce_macrophage)) -sce_macrophage <- embedContrastResults(res, - sce_macrophage, - name = "IFNgTRUE.SL1344TRUE.DESeq2" -) - -sce_macrophage - -## Run limma-trend -dge <- tximeta::makeDGEList(seg) -dge <- dge[rownames(dds), ] -logCPM <- edgeR::cpm(dge, log = TRUE, prior.count = 3) -design <- model.matrix(~ IFNg * SL1344, data = dge$samples) -fit <- limma::lmFit(logCPM, design = design) -fit <- eBayes(fit, trend = TRUE) -tt <- topTable(fit, coef = ncol(design), number = Inf, sort.by = "none") - -## Embed the results obtained from limma into the SCE -stopifnot(rownames(tt) == rownames(sce_macrophage)) -sce_macrophage <- embedContrastResults(tt, - sce_macrophage, - name = "IFNgTRUE.SL1344TRUE.limma", - class = "limma" -) - -sce_macrophage - -## Where's the information stored? -rowData(sce_macrophage)$iSEEde -``` - -## Perform pathway analysis - -We also run `r Biocpkg("fgsea")` to perform a gene set enrichment analysis of GO terms, based on the `DESeq2` results obtained above. - -```{r runfgsea} -## Extract GO term composition -pathways <- select(org.Hs.eg.db, keys(org.Hs.eg.db, "SYMBOL"), c("GOALL"), - keytype = "SYMBOL" -) -pathways <- subset(pathways, ONTOLOGYALL == "BP") -pathways <- unique(pathways[, c("SYMBOL", "GOALL")]) -pathways <- split(pathways$SYMBOL, pathways$GOALL) -len_pathways <- lengths(pathways) -pathways <- pathways[len_pathways > 15 & len_pathways < 200] -length(pathways) - -## Get test statistics and apply fgsea -feature_stats <- res$stat -names(feature_stats) <- rownames(res) -set.seed(42) -fgseaRes <- fgsea( - pathways = pathways, - stats = feature_stats, - minSize = 15, - maxSize = 200 -) -fgseaRes <- fgseaRes[order(pval), ] - -head(fgseaRes) - -## Embed pathway analysis results in the SCE object -sce_macrophage <- embedPathwaysResults(fgseaRes, - sce_macrophage, - name = "IFNgTRUE.SL1344TRUE.DESeq2.fgsea", - class = "fgsea", - pathwayType = "GO", - pathwaysList = pathways, - featuresStats = feature_stats -) - -sce_macrophage -## Again: where's the data stored? -metadata(sce_macrophage)$iSEEpathways - -## Add details about the GO terms -## These will be displayed in the application -go_details <- function(x) { - info <- select(GO.db, x, c("TERM", "ONTOLOGY", "DEFINITION"), "GOID") - html <- list(p(strong(info$GOID), ":", info$TERM, paste0("(", info$ONTOLOGY, ")"))) - if (!is.na(info$DEFINITION)) { - html <- append(html, list(p(info$DEFINITION))) - } - tagList(html) -} - -## Define the mapping from GO terms to gene IDs -map_GO <- function(pathway_id, se) { - pathway_symbol <- mapIds(org.Hs.eg.db, pathway_id, "SYMBOL", - keytype = "GOALL", multiVals = "CharacterList" - )[[pathway_id]] - pathway_rownames <- rownames(se)[rowData(se)$SYMBOL %in% pathway_symbol] - pathway_rownames -} - -## Register the pathway mapping information into the SCE object -sce_macrophage <- registerAppOptions(sce_macrophage, Pathways.map.functions = list(GO = map_GO)) -sce_macrophage <- registerAppOptions(sce_macrophage, PathwaysTable.select.details = go_details) - -metadata(sce_macrophage)$iSEE -``` - -This concludes the preparation of the dataset for this workshop. -We can conveniently store that as a binary RDS file which we can load afterwards, anytime. - -```{r savemac} -saveRDS(sce_macrophage, "datasets/sce_macrophage_readytouse.RDS") -``` - - -# Session info {-} - -```{r sessioninfo} -sessionInfo() -``` - diff --git a/inst/datasets/README.md b/inst/datasets/README.md deleted file mode 100644 index e69de29..0000000 diff --git a/inst/datasets/sce_macrophage_readytouse.RDS b/inst/datasets/sce_macrophage_readytouse.RDS deleted file mode 100644 index 705a32f..0000000 Binary files a/inst/datasets/sce_macrophage_readytouse.RDS and /dev/null differ diff --git a/inst/datasets/sce_pbmc3k.RDS b/inst/datasets/sce_pbmc3k.RDS deleted file mode 100644 index d29de44..0000000 Binary files a/inst/datasets/sce_pbmc3k.RDS and /dev/null differ diff --git a/link.svg b/link.svg new file mode 100644 index 0000000..88ad827 --- /dev/null +++ b/link.svg @@ -0,0 +1,12 @@ + + + + + + diff --git a/man/iUSEiSEE-pkg.Rd b/man/iUSEiSEE-pkg.Rd deleted file mode 100644 index b44a3af..0000000 --- a/man/iUSEiSEE-pkg.Rd +++ /dev/null @@ -1,32 +0,0 @@ -% Generated by roxygen2: do not edit by hand -% Please edit documentation in R/iUSEiSEE-pkg.R -\docType{package} -\name{iUSEiSEE-pkg} -\alias{iUSEiSEE} -\alias{iUSEiSEE-package} -\alias{iUSEiSEE-pkg} -\title{iUSEiSEE: Learning to use the package iSEE} -\description{ -This package provides the material for a fully fledged one-day workshop on the iSEE package. -} -\seealso{ -Useful links: -\itemize{ - \item \url{https://imbeimainz.github.io/iUSEiSEE/} - \item Report bugs at \url{https://github.com/imbeimainz/iUSEiSEE/issues} -} - -} -\author{ -\strong{Maintainer}: Federico Marini \email{marinif@uni-mainz.de} (\href{https://orcid.org/0000-0003-3252-7758}{ORCID}) - -Authors: -\itemize{ - \item Kevin Rue-Albrecht \email{kevinrue67@gmail.com} (\href{https://orcid.org/0000-0003-3899-3872}{ORCID}) - \item Charlotte Soneson \email{charlottesoneson@gmail.com} (\href{https://orcid.org/0000-0003-3833-2169}{ORCID}) - \item Aaron Lun \email{infinite.monkeys.with.keyboards@gmail.com} (\href{https://orcid.org/0000-0002-3564-4813}{ORCID}) - \item Najla Abassi \email{abassi.nejla96@gmail.com} (\href{https://orcid.org/0000-0001-8357-0938}{ORCID}) -} - -} -\keyword{internal} diff --git a/pkgdown.css b/pkgdown.css new file mode 100644 index 0000000..80ea5b8 --- /dev/null +++ b/pkgdown.css @@ 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    iUSEiSEE: Learning to use the package iSEE

    + Source: R/iUSEiSEE-pkg.R + +
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    This package provides the material for a fully fledged one-day workshop on the iSEE package.

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    Author

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    Maintainer: Federico Marini marinif@uni-mainz.de (ORCID)

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    Authors:

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    Developed by Federico Marini, Kevin Rue-Albrecht, Charlotte Soneson, Aaron Lun, Najla Abassi.

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    Site built with pkgdown 2.0.9.

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    Reference

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    Developed by Federico Marini, Kevin Rue-Albrecht, Charlotte Soneson, Aaron Lun, Najla Abassi.

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    Site built with pkgdown 2.0.9.

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    + + + + + + + + diff --git a/sitemap.xml b/sitemap.xml new file mode 100644 index 0000000..d3a1cf8 --- /dev/null +++ b/sitemap.xml @@ -0,0 +1,42 @@ + + + + https://imbeimainz.github.io/iUSEiSEE/404.html + + + https://imbeimainz.github.io/iUSEiSEE/LICENSE-text.html + + + https://imbeimainz.github.io/iUSEiSEE/LICENSE.html + + + https://imbeimainz.github.io/iUSEiSEE/articles/about_this_workshop_00.html + + + https://imbeimainz.github.io/iUSEiSEE/articles/bonus_content_04.html + + + https://imbeimainz.github.io/iUSEiSEE/articles/iSEE_overview_02.html + + + https://imbeimainz.github.io/iUSEiSEE/articles/iSEE_quickrecipes_03.html + + + https://imbeimainz.github.io/iUSEiSEE/articles/iUSEiSEE_01.html + + + https://imbeimainz.github.io/iUSEiSEE/articles/index.html + + + https://imbeimainz.github.io/iUSEiSEE/authors.html + + + https://imbeimainz.github.io/iUSEiSEE/index.html + + + https://imbeimainz.github.io/iUSEiSEE/reference/iUSEiSEE-pkg.html + + + https://imbeimainz.github.io/iUSEiSEE/reference/index.html + + diff --git a/vignettes/about_this_workshop_00.Rmd b/vignettes/about_this_workshop_00.Rmd deleted file mode 100644 index 9483d14..0000000 --- a/vignettes/about_this_workshop_00.Rmd +++ /dev/null @@ -1,105 +0,0 @@ ---- -title: "iUSEiSEE - About this workshop" -author: "Federico Marini^[marinif@uni-mainz.de], Najla Abassi^[abassina@uni-mainz.de]
    - Collection of material developed by the iSEE core dev team
    - (Kevin Rue-Albrecht, Federico Marini, Charlotte Soneson, Aaron Lun)" -output: - html_document: - theme: cosmo - toc: yes - toc_float: yes - number_sections: true -vignette: > - %\VignetteIndexEntry{00. About this workshop} - %\VignettePackage{iUSEiSEE} - %\VignetteEngine{knitr::rmarkdown} - %\VignetteEncoding{UTF-8} -editor_options: - chunk_output_type: console -bibliography: iUSEiSEErefs.bib ---- - - -```{r, include = FALSE} -knitr::opts_chunk$set( - collapse = TRUE, - comment = "#>" -) -suppressPackageStartupMessages({ - library("BiocStyle") -}) -``` - - - - -# Description - -The iSEE package [@Rue-Albrecht2018-isee] is a flexible, powerful and extendible application to explore any dataset stored in SummarizedExperiment objects, including single cell, spatially resolved data, but also bulk data (transcriptomics and proteomics being just two examples of what can efficiently fit in there!). - -iSEE enables a multitude of panels to gain in-depth insight in your data; it does so combining interactivity and reproducibility, and providing an ideal companion to other tools (such as CELLxGENE) for deeper dives into data. - -This package provides a complete overview of the functionality of the `r Biocpkg("iSEE")` Bioconductor package, and is intended as some kind of "masterclass-level" workshop - indeed, the material is enough to cover a full-day workshop on iSEE. - -# Pre-requisites - -* Familiarity with bulk or single-cell sequencing data (ideally scRNA-seq) -* Familiarity with the SummarizedExperiment/SingleCellExperiment class, or -alternatively the Seurat or AnnData frameworks - basically, any of the widely used -ecosystem for working with/storing/processing single cell data -* Familiarity with single-cell data visualizations and representations - -# Participation - -Participants will be able (and heartily invited) to follow along in the live coding parts of the workshop. This is very much intended as a hands-on learning resource. - -Given the hands-on nature of this, this is going to be a BYOD (Bring Your Own Device) event - if cloud-based setups are provided, this information will be passed on in advance to the participants. -Nonetheless, we value the side effect of participants installing a working environment as a part of the learning objectives. - -Participants will (likely) use a set of stickers to indicate their progress throughout the material, to be attached onto the laptop lid. In brief: - -* Yellow sticker: "I am working on the task" -* Green sticker: "I am done with the task" -* Red sticker: "I am stuck on this, could you please come help me?" - -We do stress the importance of working together on such tasks, therefore participants are very encouraged to talk to their neighbors during the task sessions! - -# _R_ / _Bioconductor_ packages used - -* `iSEE`: https://bioconductor.org/packages/iSEE -* `iSEEu`: https://bioconductor.org/packages/iSEEu -* `iSEEde`: https://bioconductor.org/packages/iSEEde -* `iSEEpathways`: https://bioconductor.org/packages/iSEEpathways -* `iSEEhub`: https://bioconductor.org/packages/iSEEhub -* `iSEEindex`: https://bioconductor.org/packages/iSEEindex -* `iSEEfier`: https://bioconductor.org/packages/iSEEfier - - -# Outline of the course - -After a short presentation, we will start going through the material in the `vignettes` folder: - -* [iUSEiSEE](iUSEiSEE_01.Rmd) - a tl;dr version of the whole, which can be used as a compact summary of the content -* [Overview on iSEE](iSEE_overview_02.Rmd) - a detailed overview on iSEE and its functionality -* [The iSEE cookbook](iSEE_quickrecipes_03.Rmd) - a set of self-contained recipes with simple-to-advanced tasks one can complete within iSEE -* [Bonus advanced content around iSEE](bonus_content_04.Rmd) - some additional content, focused on some advanced or more specific tasks to carry out with iSEE - -# Workshop goals and objectives - -## Learning goals - -* Outline the features of iSEE, built upon the RStudio Shiny framework -* Utilize interactive GUI components and layouts to efficiently extract information from biological data sets -* Describe how to construct interactive apps and custom panels -* Demonstrate how these package can smoothly be integrated into and enhance existing workflows for the datasets at hand - -## Learning objectives - -* Set up a local environment for running iSEE apps -* Interact with components of the iSEE user interface to visually inspect and discuss various data sets -* Identify and locate configurable aspects of iSEE apps -* Practice interactive visualization over a single-cell RNA-sequencing workflow -* Imagine use cases and future developments for interactive visualization as part of computational workflows -* Design and create iSEE instances for collections of datasets - -# References {-} diff --git a/vignettes/bonus_content_04.Rmd b/vignettes/bonus_content_04.Rmd deleted file mode 100644 index e61c713..0000000 --- a/vignettes/bonus_content_04.Rmd +++ /dev/null @@ -1,395 +0,0 @@ ---- -title: "iUSEiSEE - Bonus content" -author: "Federico Marini^[marinif@uni-mainz.de], Najla Abassi^[abassina@uni-mainz.de]
    - Collection of material developed by the iSEE core dev team
    - (Kevin Rue-Albrecht, Federico Marini, Charlotte Soneson, Aaron Lun)" -output: - html_document: - theme: cosmo - toc: yes - toc_float: yes - number_sections: true -vignette: > - %\VignetteIndexEntry{04. Bonus content} - %\VignettePackage{iUSEiSEE} - %\VignetteEncoding{UTF-8} - %\VignetteEngine{knitr::rmarkdown} -editor_options: - chunk_output_type: console -bibliography: iUSEiSEErefs.bib ---- - - - -```{r setup, include=FALSE} -knitr::opts_chunk$set( - echo = TRUE, - cache = FALSE, - collapse = TRUE, - comment = "#>", - crop = NA -) -suppressPackageStartupMessages({ - library("BiocStyle") - library("iSEE") -}) -htmltools::tagList(rmarkdown::html_dependency_font_awesome()) -``` - -```{r, eval=!exists("SCREENSHOT"), include=FALSE} -SCREENSHOT <- function(x, ...) knitr::include_graphics(x) -``` - - -# Moar iSEE things - -This vignette contains some examples on even more `iSEE`-things [@Rue-Albrecht2018-isee]. - -We will use the following packages throughout its content (make sure to have them installed if you want to run it interactively) - -```{r pkgs} -library("iSEE") -``` - - -# iSEE a challenge: let's reproduce any figure! - -We'd like to open up a challenge, with some simple rules: - -* You search a nice graphical representation of data -* We try to reproduce this within `iSEE` - as far as this is possible! - -Let's suggest a couple of figure/figure panels! -Enter a couple of suggestions in this GSheet: https://docs.google.com/spreadsheets/d/1poE713rXqzfNdPcKAPN2AxJYXhaxy8GwgAjRsk_UiK8/edit?usp=sharing - -# iSEEde and iSEEpathways - -iSEEde and iSEEpathways: ideal companions for exploring DE results - -We first load the processed `macrophage` data (derived from the work of [@Alasoo2018]) - on this, we already ran the workflow of `DESeq2` [@Love2014] to identify the differentially expressed genes. - -```{r} -macrophage_location <- system.file("datasets", "sce_macrophage_readytouse.RDS", - package = "iUSEiSEE" -) -macrophage_location - -sce_macrophage <- readRDS(macrophage_location) - -library("iSEE") -library("iSEEde") -library("iSEEpathways") -``` - -`r Biocpkg("iSEEde")` and `r Biocpkg("iSEEpathways")` are two new Bioconductor packages that provide `r Biocpkg("iSEE")` panels specifically aimed towards exploration of differential expression and pathway analysis results. -More precisely, `r Biocpkg("iSEEde")` provides the `VolcanoPlot`, `MAPlot`, `LogFCLogFCPlot` and `DETable` panels. -These panels can be configured to extract data that was added via the `embedContrastResults()` function above. -Let's look at an example: - -```{r} -app <- iSEE(sce_macrophage, initial = list( - DETable( - ContrastName = "IFNgTRUE.SL1344TRUE.DESeq2", - HiddenColumns = c("baseMean", "lfcSE", "stat") - ), - VolcanoPlot(ContrastName = "IFNgTRUE.SL1344TRUE.DESeq2"), - MAPlot(ContrastName = "IFNgTRUE.SL1344TRUE.DESeq2") -)) -``` - -```{r, echo=FALSE} -SCREENSHOT("images/isee_de_setup1.png", delay = 20) -``` - - - - - - - - -Note how it is easy to switch to a different contrast in any of the panels. - -```{r} -app <- iSEE(sce_macrophage, initial = list( - iSEEde::DETable( - ContrastName = "IFNgTRUE.SL1344TRUE.DESeq2", - HiddenColumns = c("baseMean", "lfcSE", "stat") - ), - iSEEde::VolcanoPlot(ContrastName = "IFNgTRUE.SL1344TRUE.DESeq2"), - iSEEde::MAPlot(ContrastName = "IFNgTRUE.SL1344TRUE.DESeq2"), - PathwaysTable( - ResultName = "IFNgTRUE.SL1344TRUE.limma.fgsea", - Selected = "GO:0046324" - ), - ComplexHeatmapPlot( - RowSelectionSource = "PathwaysTable1", - CustomRows = FALSE, ColumnData = "condition_name", - ClusterRows = TRUE, Assay = "vst" - ), - FgseaEnrichmentPlot( - ResultName = "IFNgTRUE.SL1344TRUE.limma.fgsea", - PathwayId = "GO:0046324" - ) -)) -``` - -```{r, echo=FALSE} -SCREENSHOT("images/isee_de_setup2.png", delay = 30) -``` - - - - - - - - - - - -# iSEEfier - -Let's say we are interested in visualizing the expression of a list of specific marker genes in one view, or maybe we created different initial states separately, but would like to visualize them in the same instance. As we previously learned, we can do a lot of these tasks by running the command: - -```{r, eval=FALSE} -# don't run -iSEE(sce) -``` - -Then, add/remove the panels to our need. This can involve multiple steps (selecting the gene of interest, color by a specific `colData`...), or probably even write multiple lines of code. -For that we can use the `r Biocpkg("iSEEfier")` package, which streamlines the process of starting (or if you will, firing up) an `iSEE` instance with a small chunk of code, avoiding the tedious way of setting up every `iSEE` panel individually. - -In this section, we will illustrate a simple example of how to use `r Biocpkg("iSEEfier")`. We will use the same pbmc3k data we worked with during this workshop. - -We start by loading the data: - -```{r} -library("iSEEfier") - -# import data -sce <- readRDS( - file = system.file("datasets", "sce_pbmc3k.RDS", package = "iUSEiSEE") -) - -sce -``` - -For example, we can be interested in visualizing the expression of *GZMB*, *TGFB*, and *CD28* genes all at once. We start by providing a couple of parameters: - -```{r} -# define the list of genes -feature_list_1 <- c("GZMB", "TGFB1", "CD28") -# define the cluster/cell type -cluster_1 <- "labels_main" -``` - -Now we can pass these parameters into `iSEEinit()` to create a customized initial configuration: - -```{r} -# create an initial state with iSEEinit -initial_1 <- iSEEinit(sce, - features = feature_list_1, - clusters = cluster_1, - add_markdown_panel = TRUE) -``` - -The rest can be as easy as passing this initial to the `iSEE()` call: - -```{r} -app <- iSEE(sce, initial = initial_1) -``` - -This is how it would look like: - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-on-fier.png", delay = 30) -``` - - -While we are visualizing the expression of these genes, we might want to take some notes (gene X is more expressed in a certain cell type/cluster than some others, maybe we are trying to annotate the cells ourselves if the annotation wasn't available...). -For this, we used the argument `add_markdown_panel = TRUE`. It will display a `MarkdownBoard` panel where we can note our observations without leaving the app. - -We can also use the argument `add_dynamicTable_panel=TRUE` to add another custom panel to display the maker genes of certain cluster/cell type. - -```{r} -feature_list_2 <- c("CD74", "CD79B") -initial_2 <- iSEEinit(sce, - features = feature_list_2, - clusters = cluster_1, - add_markdown_panel = TRUE, - add_dynamicTable_panel = TRUE) -``` - -```{r} -app <- iSEE(sce, initial = initial_2) -``` - -We can check the initial's content, or how the included panels are linked between each other without running the app with `view_initial_tiles()` and `view_initial_network()`: - -```{r} -# display a graphical representation of the initial configuration, where the panels are identified by their corresponding colors -view_initial_tiles(initial = initial_1) -# display a network visualization for the panels -view_initial_network(initial_1, plot_format = "igraph") -``` - -Another alternative for network visualization would use the interactive widget provided by `visNetwork`: - -```{r} -view_initial_network(initial_1, plot_format = "visNetwork") -``` - -It is also possible to combine multiple initials into one: - -```{r} -merged_config <- glue_initials(initial_1, initial_2) -# check out the content of merged_config -view_initial_tiles(initial = merged_config) -``` - -`?iSEEfier` is always your friend whenever you need further documentation on the package/a certain function and how to use it. - -# Multi-sample, multi-condition datasets: how to let iSEE shine - -We will take another dataset for this, from a recent work of Bartneck and colleagues ([@Bartneck2023]). - -You can find the dataset in the `sce_merged_allsets.RDS` object - let's see together how we can access that --> https://seafile.rlp.net/d/c7f1335a6248427d97da/. - -Once the data is available locally, we load it in the R session and simply start iSEE on it. - -```{r eval=FALSE} -library("iSEE") -sce_bartneck <- readRDS("path/to/sce_merged_allsets.RDS") -iSEE(sce_bartneck) -``` - -For more about this work, please refer to the [original publication](https://www.frontiersin.org/articles/10.3389/fimmu.2023.1267866/full). - -# iSEEhub & iSEEindex - -## iSEEhub: `iSEE`ing the ExperimentHub datasets - -The `r Biocpkg("iSEEhub")` package provides a custom landing page for an `r Biocpkg("iSEE")` application interfacing with the Bioconductor `r Biocpkg("ExperimentHub")`. -The landing page allows users to browse the ExperimentHub, select a data set, download and cache it, and import it directly into an iSEE app. - -```{r} -library("iSEE") -library("iSEEhub") - -ehub <- ExperimentHub() -app <- iSEEhub(ehub) -``` - -```{r, echo=FALSE} -SCREENSHOT("images/iseehub.png", delay = 20) -``` - - - - - -## iSEEindex: one instance of `iSEE` to explore them all - -`r Biocpkg("iSEEindex")` provides an interface to **any** collection of data sets within a single iSEE web-application. -The main functionality of this package is to define a custom landing page allowing app maintainers to list a custom collection of data sets that users can select from and directly load objects into an iSEE web application. -To see how to configure such an app, we will create a small example: - -```{r} -library("iSEE") -library("iSEEindex") - -bfc <- BiocFileCache(cache = tempdir()) - -dataset_fun <- function() { - x <- yaml::read_yaml(system.file(package = "iSEEindex", "example.yaml")) - x$datasets -} -initial_fun <- function() { - x <- yaml::read_yaml(system.file(package = "iSEEindex", "example.yaml")) - x$initial -} -app <- iSEEindex(bfc, dataset_fun, initial_fun) -``` - -```{r, echo=FALSE} -SCREENSHOT("images/iseeindex.png", delay = 20) -``` - - -A more elaborate example (referring to the work in [@Rigby2023]) is available at [https://rehwinkellab.shinyapps.io/ifnresource/](https://rehwinkellab.shinyapps.io/ifnresource/). -The source can be found at [https://github.com/kevinrue/IFNresource](https://github.com/kevinrue/IFNresource). - - - - - - - -Potential use cases can include: - -* An app to present and explore the different datasets in your next publication -* An app to explore collection of datasets collaboratively, in consortium-like initiatives -* An app to mirror and enhance the content of e.g. the cellxgene data portal -* Got any ideas on how to use iSEE for such deployments? - - -# Tours: help and storytelling - -Tours can be an essential tool to satisfy two needs: - -* Helping users to navigate the UI -* Telling a story on an existing configuration - -A simple example can be demonstrated with this configuration: - -```{r} -sce_location <- system.file("datasets", "sce_pbmc3k.RDS", package = "iUSEiSEE") -sce_location -sce <- readRDS(sce_location) - -initial_for_tour <- list( - ReducedDimensionPlot(PanelWidth = 3L), - RowDataTable(PanelWidth = 3L), - FeatureAssayPlot(PanelWidth = 3L), - ComplexHeatmapPlot(PanelWidth = 3L) -) -``` - -This next chunk defines the **steps** of the tour, specified by an anchoring point (`element`) and the content of that step (`intro`). - -```{r} -tour <- data.frame( - element = c( - "#Welcome", - "#ReducedDimensionPlot1", - "#RowDataTable1", - "#ComplexHeatmapPlot1", - "#FeatureAssayPlot1", - "#ReducedDimensionPlot1", - "#Conclusion"), - intro = c( - "Welcome to this tour!", - "This is the a reduced dimension plot", - "and this is a table", - "Why not a heatmap?", - "... and now we look at one individual feature.", - "Back to the a reduced dimension plot...", - "Thank you for taking this tour!"), - stringsAsFactors = FALSE -) - -app <- iSEE(sce, initial = initial_for_tour, tour = tour) -``` - -```{r, echo=FALSE} -SCREENSHOT("images/isee_simpletour.png", delay = 20) -``` - -# Session info {-} - -```{r} -sessionInfo() -``` - -# References {-} diff --git a/vignettes/iSEE_overview_02.Rmd b/vignettes/iSEE_overview_02.Rmd deleted file mode 100644 index 9e3bed0..0000000 --- a/vignettes/iSEE_overview_02.Rmd +++ /dev/null @@ -1,622 +0,0 @@ ---- -title: "iUSEiSEE - An overview of iSEE" -author: "Federico Marini^[marinif@uni-mainz.de], Najla Abassi^[abassina@uni-mainz.de]
    - Collection of material developed by the iSEE core dev team
    - (Kevin Rue-Albrecht, Federico Marini, Charlotte Soneson, Aaron Lun)" -output: - html_document: - theme: cosmo - toc: yes - toc_float: yes - number_sections: true -vignette: > - %\VignetteIndexEntry{02. Overview of iSEE} - %\VignettePackage{iUSEiSEE} - %\VignetteEngine{knitr::rmarkdown} - %\VignetteEncoding{UTF-8} -editor_options: - chunk_output_type: console -bibliography: iUSEiSEErefs.bib ---- - - - -```{r setup, include=FALSE} -knitr::opts_chunk$set( - echo = TRUE, - cache = FALSE, - collapse = TRUE, - comment = "#>", - crop = NA -) -suppressPackageStartupMessages({ - library("BiocStyle") - library("iSEE") -}) -htmltools::tagList(rmarkdown::html_dependency_font_awesome()) -``` - -```{r, eval=!exists("SCREENSHOT"), include=FALSE} -SCREENSHOT <- function(x, ...) knitr::include_graphics(x) -``` - - -# Introduction - -This vignette provides an overview of the graphical interface of `r Biocpkg("iSEE")` applications [@Rue-Albrecht2018-isee]. -To follow along, make sure that you launch the default `r Biocpkg("iSEE")` instance as described in the next code block at the start of the [following section](#default-app). - -Note that in the default configuration, the panels do not look exactly like the ones shown in the screenshots that you will see below. -For example, data points are not immediately colored, and the default annotation variables displayed by each panel may differ. -The [hands-on recipes](recipes.html) workshop of this workshop will demonstrate how to modify the content of the panels, and how they are displayed. - -Note that for simplicity, we typically refer to a `SummarizedExperiment` in this workshop; however, `r Biocpkg("iSEE")` works seamlessly for objects of any class extending `SummarizedExperiment` as well (e.g., `SingleCellExperiment`, `DESeqDataSet`). -That said, some types of panels -- such as the `r .fullName(ReducedDimensionPlot())` -- are only available for objects that contain a `reducedDim` slot (in particular, `SingleCellExperiment` objects); the basic `SummarizedExperiment` class does not contain this slot. -In this workshop, we refer to the rows of the `SummarizedExperiment` object as 'features' (these can be genes, transcripts, genomic regions, etc) and to the columns as 'samples' (which, in our example data set, are single cells). - -# The default iSEE app {#default-app} - -Using the demonstration data set, we can launch an `r Biocpkg("iSEE")` instance for exploring this data set using the `iSEE()` function without any further argument. -This will produce an app using the default configuration; that is, the app instance will include one panel of each built-in class for which the relevant information is available in the `SummarizedExperiment` object. - -```{r, message=FALSE} -library("iSEE") -sce_location <- system.file("datasets", "sce_pbmc3k.RDS", package = "iUSEiSEE") -sce_location -sce <- readRDS(sce_location) -app <- iSEE(sce) -``` - -```{r runApp, eval=FALSE} -shiny::runApp(app) -``` - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-initial.png", delay = 40) -``` - -The main argument to the `iSEE()` function is a `SummarizedExperiment` object, or an object of any class extending `SummarizedExperiment` (such as `SingleCellExperiment`, in this case). -No other restrictions are made on the type of data stored in the object, and `r Biocpkg("iSEE")` can be used for interactive visualization of many different types of data. -It is also worth noting that for various types of data, Bioconductor packages provides functionality for directly importing quantifications generated by external software packages into a `SummarizedExperiment` object. -For example, the `r Biocpkg("DropletUtils")` package can read quantifications from the 10x Genomics CellRanger pipeline for single-cell RNA-seq data, and the `r Biocpkg("tximeta")` package can be used to read data from transcript quantification pipelines into a `SummarizedExperiment` object. - -While we will not make explicit use of it in this workshop, we note that it is also possible to call `iSEE()` without providing a `SummarizedExperiment` object. -In that case, the user will be prompted to upload such an object, serialized into a `.rds` file. -It is also possible to import a specification of the initial panel setup. - -```{r} -app <- iSEE() -``` - -```{r, eval=FALSE} -shiny::runApp(app) -``` - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-no-se.png", delay = 10) -``` - -# The built-in panel types {#user-interface} - -## The panel hierarchy - -The `r Biocpkg("iSEE")` user interface (UI) consists of a number of panels, each displaying the data provided in the `SummarizedExperiment` from a specific perspective. -There are 8 standard panel types; 6 plot panels and 2 table panels, all showcased in the figure shown at the start of the [previous section](#default-app). -In the default configuration, one panel of each type is included when launching the `r Biocpkg("iSEE")` user interface. -However, users are free to rearrange, resize, remove or add panels freely, as demonstrated in the separate vignette of [workshop recipes](recipes.html). -We provide a brief overview of each panel type in the following subsections. - -In addition to the 8 standard panel types included in `r Biocpkg("iSEE")`, users can create custom panels (both plots and tables). -Moreover, the `r Biocpkg("iSEEu")` (iSEE universe) package contains additional panel types. -The creation and configuration of custom panels is also demonstrated in a series of [workshop advanced recipes](recipes.html). - -Specifically, `r Biocpkg("iSEE")` panels are implemented as a hierarchy of S4 classes. -Only _concrete_ classes (orange) may be instantiated as panels in the user interface; while _virtual_ classes (grey) provide functionality shared by families of panels (e.g., sample-oriented plots, feature-oriented tables). - -```{r, echo=FALSE, out.width='100%'} -knitr::include_graphics("images/panel-classes.png", dpi = 180) -``` - - - -## `r .fullName(ReducedDimensionPlot())` {#reddimplot} - -The reduced dimension plot can display any reduced dimension representation that is present in the `reducedDim` slot of the `SingleCellExperiment` object. - -Note that this slot is not defined for the base `SummarizedExperiment` class, in which case the user interface does not allow the inclusion of panels of this type. - -```{r, message=FALSE, echo = FALSE} -app <- iSEE(sce, initial = list(ReducedDimensionPlot( - PanelWidth = 12L, - Type = "TSNE", ColorBy = "Column data", - ColorByColumnData = "Cluster" -))) -``` - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-reduceddimensionplot.png", delay = 10) -``` - -## `r .fullName(ColumnDataPlot())` {#coldataplot} - -The column data plot can display one or two of the provided column annotations (from the `colData` slot). -Depending on the class of the selected annotations, the panel shows either a scatter plot, a violin plot, or a Hinton diagram [@Hinton1991-hintondiagram; @Bremner1994-hintonplots]. - -```{r, message=FALSE, echo = FALSE} -app <- iSEE(sce, initial = list(ColumnDataPlot( - PanelWidth = 12L, - XAxis = "Column data", XAxisColumnData = "Cluster", - YAxis = "log10_total" -))) -``` - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-columndataplot.png", delay = 10) -``` - -## `r .fullName(RowDataPlot())` {#rowdataplot} - -Analogous to the [column data plot](#coldataplot) above, the row data plot displays one or two of the provided row annotations (from the `rowData` slot). -Depending on the class of the selected annotations, the panel displays either a scatter plot, a violin plot, or a Hinton plot. - -```{r, message=FALSE, echo = FALSE} -app <- iSEE(sce, initial = list(RowDataPlot( - PanelWidth = 12L, XAxis = "Row data", - YAxis = "detected", XAxisRowData = "log10_total" -))) -``` - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-rowdataplot.png", delay = 10) -``` - -## `r .fullName(ComplexHeatmapPlot())` {#heatmap} - -The complex heatmap panel displays, for any assay, the observed values for a subset of the features across the samples. - -```{r, message=FALSE, echo = FALSE} -app <- iSEE(sce, initial = list(ComplexHeatmapPlot( - PanelWidth = 12L, Assay = "logcounts", CustomRows = TRUE, - CustomRowsText = c("CD14", "CD27", "CD52", "CD68", "CD19", "CD4"), - ColumnData = "Cluster", AssayCenterRows = TRUE -))) -``` - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-complexheatmapplot.png", delay = 10) -``` - -## `r .fullName(FeatureAssayPlot())` {#featassayplot} - -The feature assay plot displays the observed values for one feature across the samples. -It is also possible to plot the observed values for two features, in a scatter plot. - -```{r, message=FALSE, echo = FALSE} -app <- iSEE(sce, initial = list(FeatureAssayPlot( - PanelWidth = 12L, XAxis = "Column data", - XAxisColumnData = "Cluster", YAxisFeatureName = "CD27", - Assay = "logcounts", ColorBy = "Column data", - ColorByColumnData = "Cluster" -))) -``` - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-featureassayplot.png", delay = 10) -``` - -## `r .fullName(SampleAssayPlot())` {#sampassayplot} - -Analogous to the [`r .fullName(FeatureAssayPlot())`](#featassayplot) above, the `r .fullName(SampleAssayPlot())` shows the observed values for all features, for one of the samples. -It is also possible to plot the observed values for two samples, in a scatter plot. - -```{r, message=FALSE, echo = FALSE} -app <- iSEE(sce, initial = list(SampleAssayPlot( - PanelWidth = 12L, XAxis = "Sample name", - XAxisSampleName = "Cell1", YAxisSampleName = "Cell2", - PointSize = 2 -))) -``` - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-sampleassayplot.png", delay = 10) -``` - -## `r .fullName(RowDataTable())` {#rowdatatable} - -The row data table displays all information provided in the `rowData` slot of the `SummarizedExperiment` object, leveraging the interactivity provided by the `r CRANpkg("DT")` package. - -```{r, message=FALSE, echo = FALSE} -app <- iSEE(sce, initial = list(RowDataTable(PanelWidth = 12L))) -``` - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-rowdatatable.png", delay = 10) -``` - -## `r .fullName(ColumnDataTable())` {#coldatatable} - -Analogous to the [`r .fullName(RowDataTable())`](#rowdatatable) above, the column data table displays all information provided in the `colData` slot of the `SummarizedExperiment` object. - -```{r, message=FALSE, echo = FALSE} -app <- iSEE(sce, initial = list(ColumnDataTable(PanelWidth = 12L))) -``` - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-columndatatable.png", delay = 10) -``` - -# Collapsible boxes with display controls {#collapsible-boxes} - -## Data parameters {#data-parameters-box} - -Each plot panel type has a `Data parameters` collapsible box. -This box has different content for each panel type, but in all cases it lets the user control the data that is displayed in the plot. - -```{r, message=FALSE, echo = FALSE} -app <- iSEE(sce, initial = list(ColumnDataPlot( - PanelWidth = 12L, DataBoxOpen = TRUE, - XAxis = "Column data", XAxisColumnData = "Cluster", - YAxis = "log10_total" -))) -``` - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-columndataplot-dataparameters.png", delay = 10) -``` - -## Visual parameters {#visual-parameters-box} - -In contrast to the `Data parameters` collapsible box that lets users control _what_ is displayed in the plot, the `Visual parameters` box lets users control _how_ the information is displayed. - -This collapsible box contains the controls to change the size, shape, opacity, and color of the points, to facet the plot by any available categorical annotation, to subsample points for increased speed of plot rendering, and to control how legends are displayed. - -```{r, message=FALSE, echo=FALSE} -app <- iSEE(sce, initial = list(ColumnDataPlot( - PanelWidth = 12L, VisualBoxOpen = TRUE, - XAxis = "Column data", XAxisColumnData = "Cluster", - YAxis = "log10_total", - VisualChoices = c("Color", "Point", "Facet", "Shape", "Size", "Text") -))) -``` - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-columndataplot-visualparameters.png", delay = 10) -``` - -## Selection parameters {#selection-parameter-box} - -The `Selection parameters` collapsible box provides controls to transfer selections of points (features or samples) between panels. - -We demonstrate examples of point transmission in the separate vignette of [workshop recipes](recipes.html) - -```{r, message=FALSE, echo = FALSE} -app <- iSEE(sce, initial = list(ReducedDimensionPlot( - PanelWidth = 12L, SelectionBoxOpen = TRUE, - Type = "TSNE", ColorBy = "Column data", - ColorByColumnData = "Cluster" -))) -``` - -```{r, echo=FALSE, out.width='100%'} -SCREENSHOT("images/isee-reduceddimensionplot-selectionparameters.png", delay = 10) -``` - -# Additional controls {#additional-controls} - -At the top-right corner of the iSEE application, users can find additional controls for reproducibility, configuration, and help. - -```{r, echo = FALSE, eval = TRUE} -panel_organization_fig <- "images/PanelOrganization.png" -export_fig <- "images/Export.png" -documentation_fig <- "images/Documentation.png" -additional_info_fig <- "images/AdditionalInfo.png" -``` - -| | | | -|--------------|:----------:|----------------------------------------| -| Organization | `r knitr::include_graphics(panel_organization_fig, dpi = 180)` | [Organize panels](#organize-panels)
    [Examine panel chart](#examine-panel-chart) | -| Export | `r knitr::include_graphics(export_fig, dpi = 180)` | [Download panel output](#download-output)
    [Extract R code](#extract-r-code)
    [Display panel settings](#display-panel-settings) | -| Documentation | `r knitr::include_graphics(documentation_fig, dpi = 180)` | [Quick tour](#quick-tour)
    [Open the vignette](#browse-vignettes) | -| Additional info | `r knitr::include_graphics(additional_info_fig, dpi = 180)` | [About this session](#app-session-info)
    [About iSEE](#about-isee) | - -## Organize panels {#organize-panels} - -As mentioned above, the default behaviour of the `iSEE()` function is to launch an instance of the user interface that displays one panel of each of the standard types (provided the underlying data is available, e.g. for reduced dimension plots the `reducedDim` slot is required). -However, in some cases it is desirable to have multiple panels of the same type, and/or exclude some panel types. - -In order to accommodate such situations, users can add, remove, change the order of and resize all panels via the `Organization` menu in the top-right corner. - -Clicking in the selectize box listing all current panels will present you with a drop-down menu from which you can choose additional panels to add. -Similarly, panels can be removed by clicking on the icon associated with the panel name. - -Each panel can be individually resized by changing the width and height. -Note that the total width of a row in the interface is 12 units. -When the width of a panel is greater than the space available, the panel is moved to a new row. - -```{r, echo=FALSE, out.width='100%'} -knitr::include_graphics("images/panel-organization.png", dpi = 180) -``` - -## Linking panels and transmitting point selections {#examine-panel-chart} - -When exploring data, it is often useful to be able to select a subset of points and investigate different aspects of their characteristic features. -In `r Biocpkg("iSEE")`, this can be achieved by _selecting_ points in one panel, and _transmitting_ this selection to one or more other panels. - -The brushing and point selection can also be programmatically preconfigured. - -The button `Examine panel chart` display a graph that reports any active point transmission between panels in the app. - -```{r, echo=FALSE, out.width='100%'} -knitr::include_graphics("images/panel-graph.png", dpi = 180) -``` - -## Download panel output {#download-output} - -The `Download panel output` button opens a modal window listing all the current panels in the app. -Checkboxes allow users to select any subset of panels to export. -Finally, clicking the `Download` button in that modal will prompt the app to save plots to PDF files, tables to CSV files, package the set of files in a ZIP archive that users can download and save on their computer. - -```{r, echo=FALSE, out.width='100%'} -knitr::include_graphics("images/panel-download.png", dpi = 180) -``` - -## Extract R code {#extract-r-code} - -The fact that data exploration is done interactively is no reason to forego reproducibility! -To this end, `r Biocpkg("iSEE")` lets you export the exact R code used to create each of the currently visible plots. - -Importantly, the script reported by `r Biocpkg("iSEE")` contains a short preamble needed to set up variable names that are used in individual panels, including active brushes used to transfer point selections. - -```{r, echo=FALSE, out.width='100%'} -knitr::include_graphics("images/code-tracker.png", dpi = 180) -``` - -## Display panel settings {#display-panel-settings} - -It can take a great amount of time to achieve a satisfactory panel configuration. -To avoid the need to manually organize the panels each time the app is opened, `r Biocpkg("iSEE")` offers the possibility to export code that can be reused later to -programmatically specify how the app is initialized, as well as to inspect and export the current panel settings for future use. - -```{r, echo=FALSE, out.width='100%'} -knitr::include_graphics("images/panel-settings.png", dpi = 180) -``` - -## Quick tour {#quick-tour} - -One important aspect of visualization is the ability to share your insights with others. -A powerful way of easily getting people unfamiliar with your data up to speed is to provide a walkthrough of the interface and the different types of plots that are displayed. -With `r Biocpkg("iSEE")`, this can be achieved using _tours_. -To configure a tour, you need to create a text file with two columns; named `element` and `intro`, with the former describing the UI element to highlight in each step, and the latter providing the descriptive text that will be displayed. - -Clicking the `Quick tour` button launches an interactive tour of the interface using the `r BiocStyle::CRANpkg("rintrojs")` package, highlighting specific elements of the user interface, labeled with information and instructions guiding users through panels and tasks specific to individual apps. - -```{r, echo=FALSE, out.width='100%'} -knitr::include_graphics("images/quick-tour.png", dpi = 180) -``` - -You can see a live example of a more complex tour in action, visit . -The code used to create this app and the associated tour is available from . - -## Open the vignette {#browse-vignettes} - -The user interface navigation bar also includes this button to open the introductory vignette to `r Biocpkg("iSEE")` in your we browser. - -Depending on the version of `r Biocpkg("iSEE")` that you are using, this will adaptively lead you to a locally built vignette present on your computer, or the _release_ or the _devel_ version of the Bioconductor package landing page, e.g. <`r BiocStyle::Biocpkg("iSEE", vignette = "basic.html", label = paste("Bioconductor", BiocManager::version()))`>. - -## Session information {#app-session-info} - -This button displays the output of `sessionInfo()`, which is a useful piece of information to report when reporting an issue with an app. - -```{r, echo=FALSE, out.width='100%'} -knitr::include_graphics("images/session-info.png", dpi = 180) -``` - -## About iSEE {#about-isee} - -This button provides information about the authors of `r Biocpkg("iSEE")`, as well as citation information. - -If you use this package, please use the following citation information: - -> Rue-Albrecht K, Marini F, Soneson C, Lun ATL (2018). “iSEE: Interactive SummarizedExperiment Explorer.” _F1000Research_, *7*, -> 741. doi: 10.12688/f1000research.14966.1 (URL: https://doi.org/10.12688/f1000research.14966.1). - -A BibTeX entry for LaTeX users is: - -``` -@Article{, - title = {iSEE: Interactive SummarizedExperiment Explorer}, - author = {Kevin Rue-Albrecht and Federico Marini and Charlotte Soneson and Aaron T. L. Lun}, - publisher = {F1000 Research, Ltd.}, - journal = {F1000Research}, - year = {2018}, - month = {Jun}, - volume = {7}, - pages = {741}, - doi = {10.12688/f1000research.14966.1}, -} -``` - - -# Using iSEE: what can you do with it? - -In this section, we list a number of actions you can perform, possibly in a very smooth manner, with the help of iSEE. - -These are reported here as bullet points, and will be demonstrated by the instructors. - -* Organize the iSEE panels: - - Search for the `Organize panels` button. - - Try to add and remove panels, and resize the existing ones. - - Remember to click on `Apply settings` afterwards. - - See how to export the `initial` configuration of panels. - - Relaunch the application, now with the additional argument `initial` provided - -* Seeing more: - - Show two _Reduced dimension plot_ panels; one showing the PCA representation, one showing the t-SNE representation. - - Link them together - -* See the true colors of your data: - - Open the `Visual parameters` collapsible box in one of the _Reduced dimension plot_ panels, and set `Color by:` to `Column data`. - - Color the cells by `Cluster`, which contains the cluster labels that were assigned to the cells in the preprocessing step - - Color the cells by the log10-transformed number of detected genes (`log10_total`).# - - Set `Color by:` to `Feature name` in one of the _Reduced dimension plot_ panels, and select one of the genes from the dropdown menu. You can search for a gene of interest by typing in the dropdown box. - -* Highlighting & zooming: - - Set `Color by:` to `Sample name` in one of the Reduced dimension plots, and use the dropdown menu underneath to change the highlighted cell. - - Here, we reduce the point size and increase the transparency (i.e. reduce the value for the alpha attribute). - - We also apply a downsampling grid of 100 horizontal and vertical bins - - Double-click on the area selected in the first panel to zoom and display the same view as the second panel. - - Double click again anywhere in the panel to zoom out. - -* Ready, steady, go: - - Start `iSEE` in the default configuration - - Set up one _Column data plot_ and one _Row data plot_ panel - - Start `iSEE` in an empty configuration - -* iSEE links: - - Start an instance with the default set of panels and explore the different types of plots generated by the Feature assay plot panel using the choices available for the two axes. - - Select a set of points in one of the _Feature assay plot_ panels, by drawing a rectangle around them. - - Then open the _Selection parameters_ collapsible box of one of the other _Feature assay plot_ panels, and select the _Feature assay plot_ panel where you made the point selection from the dropdown menu under **Receive column selection from**. - - Note how the points corresponding to the cells that you selected in the first panel are highlighted in the receiving panel. - - You can highlight the points in different ways by changing the **Selection effect**. - - iSEE can display active links transmitting point selections between panel using the "[Examine panel chart](#additional-controls)" menu. -* Selecting things - - Get ready to know the rectangular selection and the lasso selection - - Create multiple selections - -* Let's export things! - - Click on the export icon () in the top-right corner, and select 'Display panel settings'. - - Copy all the code shown in the pop-up window. - - Close the app and paste this code in your R session. - - This defines the 'initial' list that we have composed manually in the examples above. Then launch a new instance using the following code. - - Note how the app starts in the same configuration as it was before closing. Of course, it is still possible to continue exploring the data interactively - we have only changed the starting configuration. - - On to the code for the output now! Click on the export icon () in the top-right corner, and select 'Extract the R code'. - - Copy the preamble and the code for the first _Reduced dimension plot_ from the pop-up window. Close the app and paste the code in your R session. - - Note how this recreates the first reduced dimension plot from the app. - - -# Modes and additional panels - -The `r Biocpkg("iSEEu")` ("iSEE universe") Bioconductor package defines additional custom panels and predefined 'modes' (startup configurations) that may be useful for specific applications. -Here we illustrate the use of the _reduced dimension_ mode, which will start an application with one reduced dimension panel for each reduced dimension representation in the input object. - -```{r} -library("iSEEu") -app <- modeReducedDim(sce) -``` - -```{r, eval = FALSE} -shiny::runApp(app) -``` - -```{r, echo=FALSE, out.width = '100%'} -SCREENSHOT("images/isee-iseeu-modereduceddim.png", delay=10) -``` - -Furthermore, since `r Biocpkg("iSEE")` version 2.0.0, users can leverage the implementation of panels as a hierarchy of S4 classes to rapidly extend the framework and develop new types of panels with virtually unlimited freedom of functionality. -In this framework, new panel types can be readily integrated in apps alongside built-in panel types and immediately benefit of most panel functionality with minimal effort, including the capacity to transmit selections to and from other panels. -New panels may be implemented by extending the core virtual class `Panel` directly for full control over the user interface and reactive observers; -alternatively, it is often desirable for developers to inherit from one of the concrete panel classes to get most of the essential functionality from the parent class "for free". - -Use cases demonstrating the implementation of new panels with various levels of complexity are available in the [Extending iSEE](https://isee.github.io/iSEE-book/) bookdown. -For example, the _Hexagonal reduced dimension plot_ - implemented in the `r Biocpkg("iSEEu")` package - demonstrates an alternative to the downsampling strategy, by summarizing data points into hexagonal bins. - -```{r} -app <- iSEE(sce, initial = list( - ReducedDimensionPlot(PanelWidth = 6L), - ReducedDimensionHexPlot(PanelWidth = 6L) -)) -``` - -```{r, eval = FALSE} -shiny::runApp(app) -``` -```{r, echo=FALSE, out.width = '100%'} -SCREENSHOT("images/isee-iseeu-modereduceddimhex.png", delay=10) -``` - - -Another useful mode you can use with your data, especially if using iSEE for mass cytometry data, would be the `modeGating`. -This launches an app preconfigured with multiple chain-linked feature expression plots for interactive data exploration. - -```{r} -# Select top variable genes ---- -plot_count <- 6 -rv <- rowVars(assay(sce, "logcounts")) -top_var <- head(order(rv, decreasing=TRUE), plot_count*2) -top_var_genes <- rownames(sce)[top_var] - -plot_features <- data.frame( - x=head(top_var_genes, plot_count), - y=tail(top_var_genes, plot_count), - stringsAsFactors=FALSE -) - -# launch the app itself ---- - -app <- modeGating(sce, - plotAssay = "logcounts", - features = plot_features) -``` - -```{r, eval = FALSE} -shiny::runApp(app) -``` - -```{r, echo=FALSE, out.width = '100%'} -SCREENSHOT("images/isee-iseeu-modegating.png", delay=20) -``` - -Interestingly, iSEEu also has a set of extra panels that can come in handy in different situations. -For example, `iSEEu::AggregatedDotPlot` implements an aggregated dot plot where each feature/group combination is represented by a dot. -The color of the dot scales with the mean assay value across all samples for a given group, while the size of the dot scales with the proportion of non-zero values across samples in that group. -This can be an alternative to a heatmap, and an example can be seen in the chunks below: - -```{r} -app <- iSEE( - sce, - initial = list( - AggregatedDotPlot( - Assay = "logcounts", - ColumnDataLabel = "labels_main", - CustomRowsText = top_var_genes - ) - - ) -) -``` - -```{r, echo=FALSE, out.width = '100%'} -SCREENSHOT("images/isee-iseeu-aggrodotplot.png", delay=20) -``` - - -New panels and modes may be defined in independent _R_ packages that declare `Imports: iSEE` in their DESCRIPTION file, and distributed online to create an "iSEE-verse" of interoperable packages for interactive visualization, akin to the `r CRANpkg('tidyverse')` collection of R packages for data science. -If you have ideas about additional panels and/or modes that might be useful, we welcome issues and pull requests at https://github.com/iSEE/iSEEu. - - - - -# Additional resources {#additional-resources} - -A lot of materials have been developed, from the first use of `r Biocpkg("iSEE")` to contributions of new panel classes in separate downstream packages. - -* The bookdown book on _Extending iSEE_: https://isee.github.io/iSEE-book/ -* Bioconductor landing page: https://bioconductor.org/packages/iSEE/ -* Publication (F1000Research, 2018): https://f1000research.com/articles/7-741/v1 -* Deployed examples: https://marionilab.cruk.cam.ac.uk/, code at https://github.com/iSEE/iSEE2018 - * Further deployments in the https://github.com/iSEE/iSEE_instances repo - * More and more examples: https://isee.github.io/apps.html -* Custom panel examples: https://github.com/iSEE/iSEE_custom -* Development version (bug reports etc): https://github.com/iSEE/iSEE - -# Session info {-} - -```{r} -sessionInfo() -``` - -# References {-} diff --git a/vignettes/iSEE_quickrecipes_03.Rmd b/vignettes/iSEE_quickrecipes_03.Rmd deleted file mode 100644 index 9deab11..0000000 --- a/vignettes/iSEE_quickrecipes_03.Rmd +++ /dev/null @@ -1,641 +0,0 @@ ---- -title: "iUSEiSEE - The iSEE cookbook" -author: "Federico Marini^[marinif@uni-mainz.de], Najla Abassi^[abassina@uni-mainz.de]
    - Collection of material developed by the iSEE core dev team
    - (Kevin Rue-Albrecht, Federico Marini, Charlotte Soneson, Aaron Lun)" -output: - html_document: - theme: cosmo - toc: yes - toc_float: yes - number_sections: true -vignette: > - %\VignetteIndexEntry{03. The iSEE cookbook} - %\VignettePackage{iUSEiSEE} - %\VignetteEngine{knitr::rmarkdown} - %\VignetteEncoding{UTF-8} -editor_options: - chunk_output_type: console -bibliography: iUSEiSEErefs.bib ---- - - - -```{r setup, include=FALSE} -knitr::opts_chunk$set( - echo = TRUE, - cache = FALSE, - collapse = TRUE, - comment = "#>", - crop = NA -) -suppressPackageStartupMessages({ - library("BiocStyle") - library("iSEE") -}) -htmltools::tagList(rmarkdown::html_dependency_font_awesome()) -``` - -```{r, eval=!exists("SCREENSHOT"), include=FALSE} -SCREENSHOT <- function(x, ...) knitr::include_graphics(x) -``` - - -# Introduction - -This vignette consists of a series of (independent) hands-on recipes, aimed at exploring the capabilities of `r Biocpkg("iSEE")` [@Rue-Albrecht2018-isee] both interactively and programmatically. -Each recipe consists of a short task and a screen shot of the desired appearance of the app instance that should be created. -For each recipe, we provide a set of hints, as well as detailed instructions on how to solve the task both interactively (by clicking in the app) and programmatically (by directly setting up and launching the desired app instance). - -For a general overview of the default `iSEE` panels, we refer to the [overview vignette](iSEE_overview_02.html). -For all the details about the panel classes and the associated slots, we refer to the help pages for the respective panel class (e.g., `?ReducedDimensionPlot`). - -# Prepare the session - -Before starting with the recipes, we need to load the required packages and the demo data set. - -```{r, message=FALSE} -library("iSEE") -library("iSEEu") - -sce_location <- system.file("datasets", "sce_pbmc3k.RDS", package = "iUSEiSEE") -sce_location -sce <- readRDS(sce_location) -sce -``` - -# The cookbook - -What follows is a list of 11 small self contained recipes, that try to address one specific aim - and provide accordingly a simple, targeted solution. - -## Recipe 1: Panel organisation - -Using the `pbmc3k` data set, create an app that contains only a reduced dimension plot panel, a row data plot panel and a column data plot panel. The widths of the three panels should be 3, 4 and 5 units, respectively. - -
    -Hints -
      -
    • Panels can be added, removed and resized via the `Organization` button in the top right corner of the app.
      • -
    • To pre-specify the panels to be included in the app, use the `initial` argument to `iSEE()`.
      • -
    • You can remind yourself of the list of slot names available for each panel class and their respective value using the `str()` function on any instance of a panel object, e.g. `str(ReducedDimensionPlot())`.
      • -
    • For more information about the different types of panels, see the [overview vignette](iSEE_overview_02.html) or the help pages for the respective panel classes.
      • -
    -
    - -
    -Solution (interactively) -
      -
    • First open an application with the default set of panels.
      • -```{r, eval = FALSE} -app <- iSEE(sce) -shiny::runApp(app) -``` -
    • Click on the `Organization` button in the top right corner of the app, and then click on `Organize panels`.
      • -
    • In the pop-up window that appears, click on the little `x` next to all the panels that you want to remove (all but the `Reduced dimension plot 1`, `Column data plot 1` and `Row data plot 1`).
      • -
    • Drag and drop the remaining three panel names in the correct order.
      • -
    • For each panel, set the correct panel width by modifying the value in the `Width` dropdown menu.
      • -
    • Click `Apply settings`.
      • -
    -
    - -
    -Solution (programmatically) -```{r} -app <- iSEE(sce, initial = list( - ReducedDimensionPlot(PanelWidth = 3L), - RowDataPlot(PanelWidth = 4L), - ColumnDataPlot(PanelWidth = 5L) -)) -``` -
    - -```{r, echo=FALSE} -SCREENSHOT("images/recipe_01.png", delay = 20) -``` - -## Recipe 2: Data parameters configuration - selecting columns to display - -Using the `pbmc3k` data set, visualize the cell type assignment against the cluster membership, with the aim to identify the predominant cell type in each cluster. In this case, since both cell annotations are categorical, `iSEE` will generate a so called Hinton plot. - -
    -Hints -
      -
    • Column (in this case cell) annotations can be visualized using a `ColumnDataPlot` panel.
      • -
    • The cluster labels are available in the `Cluster` column of `colData(sce)`.
      • -
    • The cell type assignments are available in the `labels_fine` column of `colData(sce)` (more coarse-grained assignments are provided in the `labels_main` column).
      • -
    • `iSEE` will automatically determine the plot type depending on the type of selected variables.
      • -
    -
    - -
    -Solution (interactively) -
      -
    • First open an application with a single `ColumnDataPlot` panel spanning the full application window)
      • -```{r, eval = FALSE} -app <- iSEE(sce, initial = list(ColumnDataPlot(PanelWidth = 12L))) -shiny::runApp(app) -``` -
    • In the `ColumnDataPlot` panel, click to expand the `Data parameters` collapsible box.
      • -
    • Under `Column of interest (Y-axis)`, select the label column (e.g., `labels_fine`).
      • -
    • Under `X-axis`, select `Column data`, and under `Column of interest (X-axis)`, select `Cluster`.
      • -
    -
    - -
    -Solution (programmatically) -```{r} -app <- iSEE(sce, list( - ColumnDataPlot( - PanelWidth = 12L, XAxis = "Column data", - YAxis = "labels_fine", XAxisColumnData = "Cluster" - ) -)) -``` -
    - -```{r, echo=FALSE} -SCREENSHOT("images/recipe_02.png", delay = 20) -``` - -## Recipe 3: Data parameters configuration - displaying multiple reduced dimension representations - -Using the `pbmc3k` data set, display both the tSNE and UMAP representations next to each other. - -
    -Hints -
      -
    • Reduced dimension representations can be displayed using `ReducedDimensionPlot` panels.
      • -
    • The reduced dimension representations in the SingleCellExperiment object can be accessed by name (`reducedDimNames(sce)` lists the available representations).
      • -
    -
    - -
    -Solution (interactively) -
      -
    • First open an application with two `ReducedDimensionPlot` panels, each spanning half the application window)
      • -```{r, eval = FALSE} -app <- iSEE(sce, initial = list( - ReducedDimensionPlot(PanelWidth = 6L), - ReducedDimensionPlot(PanelWidth = 6L) -)) -shiny::runApp(app) -``` -
    • In the first `ReducedDimensionPlot` panel, click to expand the `Data parameters` collapsible box.
      • -
    • In the `Type` selection box, choose `TSNE`.
      • -
    • In the second `ReducedDimensionPlot` panel, repeat the procedure but instead select `UMAP`.
      • -
    -
    - -
    -Solution (programmatically) -```{r} -app <- iSEE(sce, initial = list( - ReducedDimensionPlot(PanelWidth = 6L, Type = "TSNE"), - ReducedDimensionPlot(PanelWidth = 6L, Type = "UMAP") -)) -``` -
    - -```{r, echo=FALSE} -SCREENSHOT("images/recipe_03.png", delay = 20) -``` - -## Recipe 4: Data parameters configuration - displaying the expression of a specific gene across clusters - -Using the `pbmc3k` data set, plot the distribution of the logcount values for the gene `CD74` in each of the clusters. - -
    -Hints -
      -
    • Gene expression values can be displayed using a `FeatureAssayPlot` panel.
      • -
    • To select a gene, specify the ID provided as row names in the SingleCellExperiment.
      • -
    -
    - -
    -Solution (interactively) -
      -
    • First open an application with a `FeatureAssayPlot` panel, spanning the full application window)
      • -```{r, eval = FALSE} -app <- iSEE(sce, initial = list(FeatureAssayPlot(PanelWidth = 12L))) -shiny::runApp(app) -``` -
    • In the `FeatureAssayPlot` panel, click to expand the `Data parameters` collapsible box.
      • -
    • Under `Y-axis feature`, type or select `CD74`.
      • -
    • Under `X-axis`, select `Column data`, and under `Column of interest (X-axis)`, select `Cluster`.
      • -
    -
    - -
    -Solution (programmatically) -```{r} -app <- iSEE(sce, initial = list( - FeatureAssayPlot( - PanelWidth = 12L, XAxis = "Column data", - YAxisFeatureName = "CD74", XAxisColumnData = "Cluster" - ) -)) -``` -
    - -```{r, echo=FALSE} -SCREENSHOT("images/recipe_04.png", delay = 20) -``` - -## Recipe 5: Visual parameters configuration - coloring reduced dimension representations by cell annotation - -Using the `pbmc3k` data set, display two tSNE representations next to each other. In the first one, color the cells by the cluster label. In the second one, color the cells by the log10 of the total UMI count (`log10_total` column of `colData(sce)`). - -
    -Hints -
      -
    • Reduced dimension representations can be displayed using `ReducedDimensionPlot` panels.
      • -
    • The reduced dimension representations in the SingleCellExperiment object can be accessed by name (`reducedDimNames(sce)` lists the available representations).
      • -
    • The cluster labels are available in the `Cluster` column of `colData(sce)`.
      • -
    • Point attributes can be accessed in the `Visual parameters` collapsible box. To display or hide the possible options, check the corresponding checkboxes (`Color`, `Shape`, `Size`, `Point`).
      • -
    -
    - -
    -Solution (interactively) -
      -
    • First open an application with two `ReducedDimensionPlot` panels, each spanning half the application window)
      • -```{r, eval = FALSE} -app <- iSEE(sce, initial = list( - ReducedDimensionPlot(PanelWidth = 6L), - ReducedDimensionPlot(PanelWidth = 6L) -)) -shiny::runApp(app) -``` -
    • In each `ReducedDimensionPlot` panel, click to expand the `Data parameters` collapsible box and under `Type`, choose `TSNE`.
      • -
    • In the first `ReducedDimensionPlot` panel, click to expand the `Visual parameters` collapsible box.
      • -
    • Make sure that the `Color` checkbox is ticked.
      • -
    • Under `Color by`, select `Column data`.
      • -
    • In the dropdown menu that appears, select `Cluster`.
      • -
    • In the second `ReducedDimensionPlot` panel, repeat the procedure but instead select `log10_total`.
      • -
    -
    - -
    -Solution (programmatically) -```{r} -app <- iSEE(sce, initial = list( - ReducedDimensionPlot( - PanelWidth = 6L, Type = "TSNE", - ColorBy = "Column data", - ColorByColumnData = "Cluster" - ), - ReducedDimensionPlot( - PanelWidth = 6L, Type = "TSNE", - ColorBy = "Column data", - ColorByColumnData = "log10_total" - ) -)) -``` -
    - -```{r, echo=FALSE} -SCREENSHOT("images/recipe_05.png", delay = 20) -``` - -## Recipe 6: Visual parameters configuration - coloring reduced dimension representations by gene expression - -Using the `pbmc3k` data set, display two tSNE representations next to each other. In the first one, color the cells by the logcounts expression level of `CD3D`. In the second one, color the cells by the logcounts expression level of `CD79B`. - -
    -Hints -
      -
    • Reduced dimension representations can be displayed using `ReducedDimensionPlot` panels.
      • -
    • The reduced dimension representations in the SingleCellExperiment object can be accessed by name (`reducedDimNames(sce)` lists the available representations).
      • -
    • To select a gene, specify the ID provided as row names in the SingleCellExperiment.
      • -
    • Point attributes can be accessed in the `Visual parameters` collapsible box. To display or hide the possible options, check the corresponding checkboxes (`Color`, `Shape`, `Size`, `Point`).
      • -
    -
    - -
    -Solution (interactively) -
      -
    • First open an application with two `ReducedDimensionPlot` panels, each spanning half the application window)
      • -```{r, eval = FALSE} -app <- iSEE(sce, initial = list( - ReducedDimensionPlot(PanelWidth = 6L), - ReducedDimensionPlot(PanelWidth = 6L) -)) -shiny::runApp(app) -``` -
    • In each `ReducedDimensionPlot` panel, click to expand the `Data parameters` collapsible box and under `Type`, choose `TSNE`.
      • -
    • In the first `ReducedDimensionPlot` panel, click to expand the `Visual parameters` collapsible box.
      • -
    • Make sure that the `Color` checkbox is ticked.
      • -
    • Under `Color by`, select `Feature name`.
      • -
    • In the dropdown menu that appears, select or type `CD3D`.
      • -
    • In the second `ReducedDimensionPlot` panel, repeat the procedure but instead select `CD79B`.
      • -
    -
    - -
    -Solution (programmatically) -```{r} -app <- iSEE(sce, initial = list( - ReducedDimensionPlot( - PanelWidth = 6L, Type = "TSNE", - ColorBy = "Feature name", - ColorByFeatureName = "CD3D" - ), - ReducedDimensionPlot( - PanelWidth = 6L, Type = "TSNE", - ColorBy = "Feature name", - ColorByFeatureName = "CD79B" - ) -)) -``` -
    - -```{r, echo=FALSE} -SCREENSHOT("images/recipe_06.png", delay = 20) -``` - -## Recipe 7: Visual parameters configuration - changing the size and opacity of points - -Using the `pbmc3k` data set, display two tSNE representations next to each other. In the first one, set the point size to 0.5. In the second one, set the point size to 3 and the opacity to 0.2. - -
    -Hints -
      -
    • Reduced dimension representations can be displayed using `ReducedDimensionPlot` panels.
      • -
    • The reduced dimension representations in the SingleCellExperiment object can be accessed by name (`reducedDimNames(sce)` lists the available representations).
      • -
    • Point attributes can be accessed in the `Visual parameters` collapsible box. To display or hide the possible options, check the corresponding checkboxes (`Color`, `Shape`, `Size`, `Point`).
      • -
    -
    - -
    -Solution (interactively) -
      -
    • First open an application with two `ReducedDimensionPlot` panels, each spanning half the application window)
      • -```{r, eval = FALSE} -app <- iSEE(sce, initial = list( - ReducedDimensionPlot(PanelWidth = 6L), - ReducedDimensionPlot(PanelWidth = 6L) -)) -shiny::runApp(app) -``` -
    • In each `ReducedDimensionPlot` panel, click to expand the `Data parameters` collapsible box and under `Type`, choose `TSNE`.
      • -
    • In the first `ReducedDimensionPlot` panel, click to expand the `Visual parameters` collapsible box.
      • -
    • Make sure that the `Size` checkbox is ticked.
      • -
    • Under `Size by`, select `None`.
      • -
    • In the text box underneath, type 0.5.
      • -
    • In the second `ReducedDimensionPlot` panel, click to expand the `Visual parameters` collapsible box.
      • -
    • Make sure that the `Size` and `Point` checkboxes are ticked.
      • -
    • Under `Size by`, select `None`.
      • -
    • In the text box underneath, type 3.
      • -
    • Under `Point opacity`, drag the slider to 0.2.
      • -
    -
    - -
    -Solution (programmatically) -```{r} -app <- iSEE(sce, initial = list( - ReducedDimensionPlot( - PanelWidth = 6L, Type = "TSNE", - PointSize = 0.5 - ), - ReducedDimensionPlot( - PanelWidth = 6L, Type = "TSNE", - PointSize = 3, PointAlpha = 0.2 - ) -)) -``` -
    - -```{r, echo=FALSE} -SCREENSHOT("images/recipe_07.png", delay = 20) -``` - -## Recipe 8: Selection configuration - -Using the `pbmc3k` data set, display the expression (logcounts) of `CD3D` across the assigned clusters, as well as a tSNE representation colored by the cluster label. Select all cells with a logcount expression value of `CD3D` between (approximately) 0.5 and 4, and highlight these in the tSNE plot by means of transparency. - -
    -Hints -
      -
    • Reduced dimension representations can be displayed using `ReducedDimensionPlot` panels.
      • -
    • The reduced dimension representations in the SingleCellExperiment object can be accessed by name (`reducedDimNames(sce)` lists the available representations).
      • -
    • Gene expression values can be displayed using a `FeatureAssayPlot` panel.
      • -
    • To select a gene, specify the ID provided as row names in the SingleCellExperiment.
      • -
    • Transmission of selections is set up in the `Selection parameters` collapsible box.
      • -
    • Points can be selected by clicking and dragging the mouse to draw a rectangle around them, or by repeatedly clicking to make a lasso (free-form) selection.
      • -
    -
    - -
    -Solution (interactively) -
      -
    • First open an application with one `FeatureAssayPlot` panel and one `ReducedDimensionPlot` panel, each spanning half the application window)
      • -```{r, eval = FALSE} -app <- iSEE(sce, initial = list( - FeatureAssayPlot(PanelWidth = 6L), - ReducedDimensionPlot(PanelWidth = 6L) -)) -shiny::runApp(app) -``` -
    • In the `FeatureAssayPlot` panel, click to expand the `Data parameters` collapsible box and under `Y-axis feature`, type or select `CD3D`.
      • -
    • Under `X-axis`, select `Column data`, and under `X-axis column data`, select `Cluster`.
      • -
    • In the `FeatureAssayPlot` panel, use the mouse to drag a rectangle around all points with a logcount expression value (y-axis) between approximately 0.5 and 4.
      • -
    • In the `ReducedDimensionPlot` panel, click to expand the `Data parameters` collapsible box and under `Type`, choose `TSNE`.
      • -
    • In the `ReducedDimensionPlot` panel, further click to expand the `Visual parameters` collapsible box, make sure that the `Color` checkbox is ticked. Under `Color by`, select `Column data`. In the dropdown menu that appears, type or select `Cluster`.
      • -
    • In the `ReducedDimensionPlot` panel, click to expand the `Selection parameters` collapsible box.
      • -
    • Under `Receive column selection from`, select `Feature assay plot 1`.
      • -
    • Under `Selection effect`, make sure that `Transparent` is chosen.
      • -
    -
    - -
    -Solution (programmatically) -```{r} -app <- iSEE(sce, initial = list( - FeatureAssayPlot( - PanelWidth = 6L, - BrushData = list( - xmin = 0, xmax = 15, - ymin = 0.5, ymax = 4, - mapping = list(x = "X", y = "Y", group = "GroupBy"), - direction = "xy", - brushId = "FeatureAssayPlot1_Brush", - outputId = "FeatureAssayPlot1" - ), - XAxis = "Column data", - XAxisColumnData = "Cluster", YAxisFeatureName = "CD3D" - ), - ReducedDimensionPlot( - PanelWidth = 6L, Type = "TSNE", - ColorBy = "Column data", - ColorByColumnData = "Cluster", - SelectionEffect = "Transparent", - ColumnSelectionSource = "FeatureAssayPlot1" - ) -)) -``` -
    - -```{r, echo=FALSE} -SCREENSHOT("images/recipe_08.png", delay = 20) -``` - -## Recipe 9: Verifying the cell type identity of clusters - -Using the `pbmc3k` data set, create a scatter plots displaying the (logcounts) expression values of `CD79A` vs `CD74`, as well as a Hinton plot of the cluster and cell type assignment annotations. Select the cells co-expressing `CD79A` and `CD74` in the scatter plot. Which cell type/cluster(s) do these correspond to (color these points in the Hinton plot)? - -
    -Hints -
      -
    • Gene expression values can be displayed using a `FeatureAssayPlot` panel.
      • -
    • To select a gene, specify the ID provided as row names in the SingleCellExperiment.
      • -
    • Column (in this case cell) annotations can be visualized using a `ColumnDataPlot` panel.
      • -
    • The cluster labels are available in the `Cluster` column of `colData(sce)`. Coarse-grained cell type labels are available in the `labels_main` column.
      • -
    • Transmission of selections is set up in the `Selection parameters` collapsible box.
      • -
    • Points can be selected by clicking and dragging the mouse to draw a rectangle around them, or by repeatedly clicking to make a lasso (free-form) selection.
      • -
    -
    - -
    -Solution (interactively) -
      -
    • First open an application with one `FeatureAssayPlot` panel and one `ColumnDataPlot` panel, each spanning half of the application window)
      • -```{r, eval = FALSE} -app <- iSEE(sce, initial = list( - FeatureAssayPlot(PanelWidth = 6L), - ColumnDataPlot(PanelWidth = 6L) -)) -shiny::runApp(app) -``` -
    • In the `FeatureAssayPlot` panel, click to expand the `Data parameters` collapsible box.
      • -
    • Under `Y-axis feature`, type or select `CD79A`.
      • -
    • Under `X-axis`, select `Feature name`, and under `X-axis feature`, type or select `CD74`.
      • -
    • In the `ColumnDataPlot` panel, click to expand the `Data parameters` collapsible box.
      • -
    • Under `Column of interest (Y-axis)`, type or select `labels_main`.
      • -
    • Under `X-axis`, select `Column data`, and under `Column of interest (X-axis)`, select `Cluster`.
      • -
    • In the `FeatureAssayPlot` panel, use the mouse to drag a rectangle around all points co-expressing `CD79A` and `CD74`.
      • -
    • In the `ColumnDataPlot` panel, click to expand the `Selection parameters` collapsible box.
      • -
    • Under `Receive column selection from`, select `Feature assay plot 1`.
      • -
    • Under `Selection effect`, make sure that `Color` is chosen.
      • -
    -
    - -
    -Solution (programmatically) -```{r} -app <- iSEE(sce, initial = list( - FeatureAssayPlot( - PanelWidth = 6L, XAxis = "Feature name", - YAxisFeatureName = "CD79A", - XAxisFeatureName = "CD74", - BrushData = list( - xmin = 0.3, xmax = 7, - ymin = 0.3, ymax = 7, - mapping = list(x = "X", y = "Y", colour = "ColorBy"), - direction = "xy", brushId = "FeatureAssayPlot1_Brush", - outputId = "FeatureAssayPlot1" - ) - ), - ColumnDataPlot( - PanelWidth = 6L, XAxis = "Column data", - YAxis = "labels_main", - XAxisColumnData = "Cluster", - ColumnSelectionSource = "FeatureAssayPlot1", - ColumnSelectionRestrict = FALSE - ) -)) -``` -
    - -```{r, echo=FALSE} -SCREENSHOT("images/recipe_09.png", delay = 20) -``` - -## Recipe 10: Using modes from `iSEEu` - -Using the `pbmc3k` data set, load `iSEEu` and use the `modeReducedDim` mode to open an app displaying all the reduced dimension representations stored in the SingleCellExperiment object. Color the representations by the cell type assignment. - -
    -Hints -
      -
    • The cell type assignments are available in the `labels_fine` column of `colData(sce)` (more coarse-grained assignments are provided in the `labels_main` column).
      • -
    • The annotation to color by can be specified when calling `iSEEu::modeReducedDim()`.
      • -
    -
    - -
    -Solution (programmatically) -```{r} -app <- modeReducedDim(sce, colorBy = "labels_main") -``` -
    - -```{r, echo=FALSE} -SCREENSHOT("images/recipe_10.png", delay = 20) -``` - -## Recipe 11 - Including a tour - -Using the `pbmc3k` data set, display two tSNE representations next to each other. In the first one, color the cells by the logcounts expression level of `CD3D`. In the second one, color the cells by the logcounts expression level of `CD79B`. Also include a small tour that starts with a welcome message, next walks through the two panels, giving an informative message for each, and finally ends with a concluding message to the user. - -
    -Hints -
      -
    • Reduced dimension representations can be displayed using `ReducedDimensionPlot` panels.
      • -
    • The reduced dimension representations in the SingleCellExperiment object can be accessed by name (`reducedDimNames(sce)` lists the available representations).
      • -
    • To select a gene, specify the ID provided as row names in the SingleCellExperiment.
      • -
    • Point attributes can be accessed in the `Visual parameters` collapsible box. To display or hide the possible options, check the corresponding checkboxes (`Color`, `Shape`, `Size`, `Point`).
      • -
    • Tours are provided via the `tour` argument to `iSEE()`.
      • -
    • A tour is defined by a two-column data frame, with columns named `element` and `intro`. The `element` column contains the names of UI elements, prefixed by a hash sign. More details, including how to find the name of a particular UI elements, can be found in the `Configuring iSEE apps` vignette of `iSEE`.
      • -
    -
    - -
    -Solution (programmatically) -```{r} -tour <- data.frame( - element = c( - "#Welcome", - "#ReducedDimensionPlot1", - "#ReducedDimensionPlot2", - "#Conclusion" - ), - intro = c( - "Welcome to this tour!", - "This is the first reduced dimension plot", - "And here is the second one", - "Thank you for taking this tour!" - ), - stringsAsFactors = FALSE -) - -app <- iSEE(sce, - initial = list( - ReducedDimensionPlot( - PanelWidth = 6L, Type = "TSNE", - ColorBy = "Feature name", - ColorByFeatureName = "CD3D" - ), - ReducedDimensionPlot( - PanelWidth = 6L, Type = "TSNE", - ColorBy = "Feature name", - ColorByFeatureName = "CD79B" - ) - ), - tour = tour -) -``` -
    - -```{r, echo=FALSE} -SCREENSHOT("images/recipe_11.png", delay = 20) -``` - -# Session info {-} - -```{r} -sessionInfo() -``` - -# References {-} diff --git a/vignettes/iUSEiSEE_01.Rmd b/vignettes/iUSEiSEE_01.Rmd deleted file mode 100644 index 6b74405..0000000 --- a/vignettes/iUSEiSEE_01.Rmd +++ /dev/null @@ -1,370 +0,0 @@ ---- -title: "iUSEiSEE - The iSEE package for efficient, interactive, and reproducible exploration of data" -author: "Federico Marini^[marinif@uni-mainz.de], Najla Abassi^[abassina@uni-mainz.de]
    - Collection of material developed by the iSEE core dev team
    - (Kevin Rue-Albrecht, Federico Marini, Charlotte Soneson, Aaron Lun)" -output: - html_document: - theme: cosmo - toc: yes - toc_float: yes - number_sections: true -vignette: > - %\VignetteIndexEntry{01. iUSEiSEE} - %\VignettePackage{iUSEiSEE} - %\VignetteEngine{knitr::rmarkdown} - %\VignetteEncoding{UTF-8} -editor_options: - chunk_output_type: console -bibliography: iUSEiSEErefs.bib ---- - - - -```{r setup, include = FALSE} -knitr::opts_chunk$set( - echo = TRUE, - cache = FALSE, - collapse = TRUE, - comment = "#>", - crop = NA -) -suppressPackageStartupMessages({ - library("BiocStyle") - library("iSEE") -}) -``` - - -# `iUSEiSEE`: a masterclass-level workshop on iSEE - -The iSEE package [@Rue-Albrecht2018-isee] is a flexible, powerful and extendible application to explore any dataset stored in SummarizedExperiment objects, including single cell and spatially resolved data. - -This workshop and associated workshop package contain all the materials you might need to have a full experience and learn how to use iSEE, hands on, starting from the basics and making your way up to the more advanced features. - -This vignette contains a compact version of the more extended material of all other detailed vignettes. -Feel free to use this as a compact index for the rest! - -In brief, you will... - -* Install all required packages and software -* Go get some data -* See how easy it is to run `iSEE` -* Obtain a quick overview of the panels and the user interface -* Get to know the more advanced features of `iSEE` -* Be able to use `iSEE` on your own data (and maybe even deploy that!) - -Ready? Let's go! - -# Installing and loading the required packages - -To run the content presented in this demo, run this following chunk in your R environment. - -```{r eval = FALSE} -library("BiocManager") -BiocManager::install("imbeimainz/iUSEiSEE", dependencies = TRUE, build_vignettes = TRUE) -``` - -We start by loading the packages that will be used during the demo. - -```{r, message=FALSE, warning=FALSE} -library("SingleCellExperiment") -library("iSEE") -library("TENxPBMCData") - -library("iSEEu") - -library("iSEEde") -library("iSEEpathways") - -library("iSEEhub") -library("iSEEindex") -``` - -All of these would be loaded if you run - -```{r} -library("iUSEiSEE") -``` - -You can also clone this repository (https://imbeimainz.github.io/iUSEiSEE) locally by `git clone` - it makes it easy to follow along this vignette! - -# Let's go get us some data! - -We will start very much *in medias res*, assuming that this might be the ideal point where you have processed your data - early or late phase - and now want to have the BEST look at that. - -Navigate to https://zenodo.org/records/10084595 and download the data (it contains the three objects you might be "coming from"). -Unpack the archive you downloaded in the `datasets` subfolder of the `vignette` folder. - -We included a `SingleCellExperiment` object on a processed version for the PBMC3k dataset, many of you can somehow be familiar with this. - -Let's load the dataset in our environment: - -```{r} -sce_pbmc3k <- readRDS( - file = system.file("datasets", "sce_pbmc3k.RDS", package = "iUSEiSEE") -) - -sce_pbmc3k -``` - - -# As easy as saying `iSEE(sce)`! - -Who of you is investing a fair amount/lot of time in doing proper data exploration? - -Who of you is familiar with the beautiful CELLxGENE tool? - -Who of you dreamed to do even more on your data? - -I have a quiz for you - have a look at this, for example: - -http://shiny.imbei.uni-mainz.de:3838/iSEE_Tonsils_CD4Tcells/ - -```{r, echo=FALSE} -knitr::include_graphics("images/ss_Tcells.png") -``` - -
    -Question 1 -How many lines of code are required to realize this? - -A) 4-5 -B) 250-300 -C) 7500-10000 -
    - - -
    -Question 2 -How many of these lines had to be typed by a human? - -A) 4-5 -B) 250-300 -C) 7500-10000 -
    - -So, yes, I will show you that is as easy as that: - -```{r, eval=FALSE} -library("iSEE") -iSEE(sce_pbmc3k) -``` - -Let's run this together and see what we have got in store. - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -# iSEE, "tl;dr" - -## Quick tour of the panels - -What typical visualizations people would you use to represent and explore your datasets? - -Each panel has some essential content: - -* Data parameters -* Visual parameters -* Selection parameters - -Let's have a closer look at that on the Reduced Dimension Plot 1 panel. - -If you have no idea of what a panel can "normally" do, look for the question mark icon, that will start a tiny tour on its functionality. - -## Additional UI functionality - -iSEE also has some additional controls, grouped into the header bar, let's have a look at them: - -* Organization buttons -* Export buttons -* Documentation buttons -* Extra info buttons - -With this, we can... - -- reorganize the panel layout - add, remove, re-order -- extract the code to exactly reproduce the outputs, 1:1 -- store (and re-utilize) the panel settings -- generate at once an archive with all/selected parts of the panel outputs -- start a tour on the content displayed - yes, you can very much tell a story with iSEE! - -## In depth exploration of data - iSEE's main functionality - -### You can control pretty much everything in a plot - -Coloring by, faceting, zooming, hovering! -Make a wish! - -### You can link any panel to any other panel - -Brush selection, lasso selection, select the receiving panel, iterate! -Read out information, see the panel transmission graph. - -### You can use tables to navigate panels - -Search and select, transmit, receive, iterate! - -### Configuring your iSEE instance - -Panels: add, move, remove; exporting the `initial` configuration, reloading it - -### Reproducibility for the win! - -A magic wand: two clicks away from all the R code you need; export all outputs, save individual images - -### Sharing is caring, and so is telling a story - -Start the tour, highlighting elements; make your data really explorable! - -## Custom panels & the iSEE universe - -A couple of additional things you can do with custom panels (and this is just an appetizer!): - -* Custom panels: perform some extra steps -* Multiple selection and "DE on the fly" -* Check out `r Biocpkg("iSEEu")` for more -* Spatial data? We can already handle that with a workaround! See https://moffittlab.connect.hms.harvard.edu/merfish/merfish_homepage.html for an example - -## Got data? - -### Got single cell data? - -You might have got the gist: - -```{r, eval=FALSE} -sce <- sce_pbmc3k # Load your data in some manner -library("iSEE") -iSEE(sce) -``` - -So, yes, we mighe be down to THREE lines of code. - -### Got some special feature in your data? - -Maybe `r Biocpkg("iSEEu")` can be the place to find already that piece of functionality! - -### Got bulk? - -Have a look at the functionality provided by - -* `r Biocpkg("iSEEde")` -* `r Biocpkg("iSEEpathways")` - -These can be ideal companions for exploring DE results. - -`r Biocpkg("iSEEde")` and `r Biocpkg("iSEEpathways")` are two new Bioconductor packages that provide `r Biocpkg("iSEE")` panels specifically aimed towards exploration of differential expression and pathway analysis results. -More precisely, `r Biocpkg("iSEEde")` provides the `VolcanoPlot`, `MAPlot`, `LogFCLogFCPlot` and `DETable` panels. - -### Got many datasets? - -Have a look at the functionality provided by - -* `r Biocpkg("iSEEhub")` -* `r Biocpkg("iSEEindex")` - -The `r Biocpkg("iSEEhub")` package provides a custom landing page for an `r Biocpkg("iSEE")` application interfacing with the Bioconductor `r Biocpkg("ExperimentHub")`. -The landing page allows users to browse the ExperimentHub, select a data set, download and cache it, and import it directly into an iSEE app. - -`r Biocpkg("iSEEindex")` provides an interface to **any** collection of data sets within a single iSEE web-application. -The main functionality of this package is to define a custom landing page allowing app maintainers to list a custom collection of data sets that users can select from and directly load objects into an iSEE web application. - -> One iSEE instance to ~~rule~~ exlore them all... - -A more elaborate example is available at [https://rehwinkellab.shinyapps.io/ifnresource/](https://rehwinkellab.shinyapps.io/ifnresource/). -The source can be found at [https://github.com/kevinrue/IFNresource](https://github.com/kevinrue/IFNresource). - - - - - - - -Potential use cases can include: - -* An app to present and explore the different datasets in your next publication -* An app to explore collection of datasets collaboratively, in consortium-like initiatives -* An app to mirror and enhance the content of e.g. the CellxGene data portal -* Got any ideas on how to use iSEE for such deployments? - -## Interoperability - -"Wait, I got Seurat/Anndata objects". - -The single cell communities got you covered: - -### From Seurat... - -```{r eval=FALSE} -library("Seurat") - -seurat_object <- readRDS("datasets/seurat_pbmc3k.RDS") -sce_from_seurat <- Seurat::as.SingleCellExperiment( - seurat_object -) -sce_from_seurat - - -iSEE(sce_from_seurat) -``` - -### From AnnData... - -... which is basically able to serve any dataset available throught the CELLxGENE Data Portal! - -```{r eval=FALSE} -library("zellkonverter") - -sce_from_anndata <- zellkonverter::readH5AD(file = "datasets/anndata_pbmc3k.h5ad") -sce_from_anndata - -iSEE(sce_from_anndata) -``` - -An example could be... - -https://cellxgene.cziscience.com/e/c7856243-c59a-4b70-8ce7-25b94c2d9da1.cxg/ - - -## I want moaaaaar on iSEE! - -For more extensive descriptions of the different parts of the interface, we refer to the [overview](iSEE_overview_02.Rmd) vignette in this workshop material package on `r Biocpkg("iSEE")`. - -Additional material can also be found at these other repositories: - -* https://isee.github.io/iSEEWorkshop2020/ -* https://isee.github.io/iSEEWorkshopEuroBioc2020/ -* https://isee.github.io/iSEEDemoEuroBioC2023/ - -# Session info {-} - -```{r} -sessionInfo() -``` diff --git a/vignettes/iUSEiSEErefs.bib b/vignettes/iUSEiSEErefs.bib deleted file mode 100644 index 3784254..0000000 --- a/vignettes/iUSEiSEErefs.bib +++ /dev/null @@ -1,132 +0,0 @@ -@ARTICLE{Hinton1991-hintondiagram, - title = "Lesioning an Attractor Network: Investigations of Acquired - Dyslexia", - author = "Hinton, Geoffrey E and Shallice, Tim", - journal = "Psychological Review", - volume = 98, - number = 1, - pages = "74--95", - year = 1991 -} - - -@ARTICLE{Bremner1994-hintonplots, - title = "Hinton diagrams: Viewing connection strengths in neural networks", - author = "Bremner, Frederick J and Gotts, Stephen J and Denham, Dina L", - journal = "Behav. Res. Methods Instrum. Comput.", - volume = 26, - number = 2, - pages = "215--218", - month = jun, - year = 1994 -} - - -@ARTICLE{Huber2015-orchestrating, - title = "Orchestrating high-throughput genomic analysis with Bioconductor", - author = "Huber, Wolfgang and Carey, Vincent J and Gentleman, Robert and - Anders, Simon and Carlson, Marc and Carvalho, Benilton S and - Bravo, Hector Corrada and Davis, Sean and Gatto, Laurent and - Girke, Thomas and Gottardo, Raphael and Hahne, Florian and - Hansen, Kasper D and Irizarry, Rafael a and Lawrence, Michael - and Love, Michael I and Macdonald, James and Obenchain, Valerie - and Ole{\'s}, Andrzej K and Pag{\`e}s, Herv{\'e} and Reyes, - Alejandro and Shannon, Paul and Smyth, Gordon K and Tenenbaum, - Dan and Waldron, Levi and Morgan, Martin", - journal = "Nat. Methods", - publisher = "Nature Publishing Group", - volume = 12, - number = 2, - pages = "115--121", - year = 2015 -} - - -@ARTICLE{Rue-Albrecht2018-isee, - title = "{iSEE}: Interactive {SummarizedExperiment} Explorer", - author = "Rue-Albrecht, Kevin and Marini, Federico and Soneson, Charlotte - and Lun, Aaron T L", - abstract = "Data exploration is critical to the comprehension of large - biological data sets generated by high-throughput assays such as - sequencing. However, most existing tools for interactive - visualisation are limited to specific assays or analyses. Here, - we present the iSEE (Interactive SummarizedExperiment Explorer) - software package, which provides a general visual interface for - exploring data in a SummarizedExperiment object. iSEE is directly - compatible with many existing R/Bioconductor packages for - analysing high-throughput biological data, and provides useful - features such as simultaneous examination of (meta)data and - analysis results, dynamic linking between plots and code tracking - for reproducibility. We demonstrate the utility and flexibility - of iSEE by applying it to explore a range of real transcriptomics - and proteomics data sets.", - journal = "F1000Res.", - volume = 7, - month = jun, - year = 2018 -} - -@article{Bartneck2023, -abstract = {Tumor development and progression is shaped by the tumor microenvironment (TME), a heterogeneous assembly of infiltrating and resident host cells, their secreted mediators and intercellular matrix. In this context, tumors are infiltrated by various immune cells with either pro-tumoral or anti-tumoral functions. Recently, we published our non-invasive immunization platform DIVA suitable as a therapeutic vaccination method, further optimized by repeated application (DIVA 2 ). In our present work, we revealed the therapeutic effect of DIVA 2 in an MC38 tumor model and specifically focused on the mechanisms induced in the TME after immunization. DIVA 2 resulted in transient tumor control followed by an immune evasion phase within three weeks after the initial tumor inoculation. High-dimensional flow cytometry analysis and single-cell mRNA-sequencing of tumor-infiltrating leukocytes revealed cytotoxic CD8 + T cells as key players in the immune control phase. In the immune evasion phase, inflammatory CCR2 + PDL-1 + monocytes with immunosuppressive properties were recruited into the tumor leading to suppression of DIVA 2 -induced tumor-reactive T cells. Depletion of CCR2 + cells with specific antibodies resulted in prolonged survival revealing CCR2 + monocytes as important for tumor immune escape in the TME. In summary, the present work provides a platform for generating a strong antigen-specific primary and memory T cell immune response using the optimized transcutaneous immunization method DIVA 2 . This enables protection against tumors by therapeutic immune control of solid tumors and highlights the immunosuppressive influence of tumor infiltrating CCR2 + monocytes that need to be inactivated in addition for successful cancer immunotherapy.}, -author = {Bartneck, Joschka and Hartmann, Ann-Kathrin and Stein, Lara and Arnold-Schild, Danielle and Klein, Matthias and Stassen, Michael and Marini, Federico and Pielenhofer, Jonas and Meiser, Sophie Luise and Langguth, Peter and Mack, Matthias and Muth, Sabine and Probst, Hans-Christian and Schild, Hansj{\"{o}}rg and Radsak, Markus Philipp}, -doi = {10.3389/fimmu.2023.1267866}, -file = {:Users/fede/Library/Application Support/Mendeley Desktop/Downloaded/Bartneck et al. - 2023 - Tumor-infiltrating CCR2 inflammatory monocytes counteract specific immunotherapy.pdf:pdf}, -issn = {1664-3224}, -journal = {Frontiers in Immunology}, -mendeley-groups = {BIUM{\_}publications,CVpubs{\_}curated}, -month = {oct}, -number = {October}, -pages = {1--15}, -title = {{Tumor-infiltrating CCR2+ inflammatory monocytes counteract specific immunotherapy}}, -url = {https://www.frontiersin.org/articles/10.3389/fimmu.2023.1267866/full}, -volume = {14}, -year = {2023} -} - -@article{Alasoo2018, -abstract = {Regulatory variants are often context specific, modulating gene expression in a subset of possible cellular states. Although these genetic effects can play important roles in disease, the molecular mechanisms underlying context specificity are poorly understood. Here, we identified shared quantitative trait loci (QTLs) for chromatin accessibility and gene expression in human macrophages exposed to IFN$\gamma$, Salmonella and IFN$\gamma$ plus Salmonella. We observed that {\~{}}60{\%} of stimulus-specific expression QTLs with a detectable effect on chromatin altered the chromatin accessibility in naive cells, thus suggesting that they perturb enhancer priming. Such variants probably influence binding of cell-type-specific transcription factors, such as PU.1, which can then indirectly alter the binding of stimulus-specific transcription factors, such as NF-$\kappa$B or STAT2. Thus, although chromatin accessibility assays are powerful for fine-mapping causal regulatory variants, detecting their downstream effects on gene expression will be challenging, requiring profiling of large numbers of stimulated cellular states and time points.}, -author = {Alasoo, Kaur and Rodrigues, Julia and Mukhopadhyay, Subhankar and Knights, Andrew J. and Mann, Alice L. and Kundu, Kousik and Hale, Christine and Dougan, Gordon and Gaffney, Daniel J.}, -doi = {10.1038/s41588-018-0046-7}, -file = {:Users/fede/Library/Containers/com.apple.Preview/Data/Downloads/s41588-018-0046-7.pdf:pdf}, -issn = {15461718}, -journal = {Nature Genetics}, -mendeley-groups = {ideal{\_}manuscript,GeneTonic{\_}manuscript}, -number = {3}, -pages = {424--431}, -publisher = {Springer US}, -title = {{Shared genetic effects on chromatin and gene expression indicate a role for enhancer priming in immune response}}, -url = {http://dx.doi.org/10.1038/s41588-018-0046-7}, -volume = {50}, -year = {2018} -} - - -@article{Love2014, -abstract = {In comparative high-throughput sequencing assays, a fundamental task is the analysis of count data, such as read counts per gene in RNA-seq, for evidence of systematic changes across experimental conditions. Small replicate numbers, discreteness, large dynamic range and the presence of outliers require a suitable statistical approach. We present DESeq2, a method for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates. This enables a more quantitative analysis focused on the strength rather than the mere presence of differential expression. The DESeq2 package is available at http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html webcite.}, -author = {Love, Michael I and Huber, Wolfgang and Anders, Simon}, -doi = {10.1186/s13059-014-0550-8}, -file = {:Users/fede/Library/Application Support/Mendeley Desktop/Downloaded/Love, Huber, Anders - 2014 - Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2.pdf:pdf}, -isbn = {1465-6906}, -issn = {1474-760X}, -journal = {Genome Biology}, -keywords = {Algorithms,Computational Biology,Computational Biology: methods,Genetic,High-Throughput Nucleotide Sequencing,Models,RNA,RNA: analysis,Sequence Analysis,Software}, -mendeley-groups = {PhDthesis,pcaExplorer{\_}manuscript,ideal{\_}manuscript,VFapplication,VF,iSEE,GeneTonic{\_}manuscript}, -month = {dec}, -number = {12}, -pages = {550}, -pmid = {25516281}, -title = {{Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2}}, -url = {http://genomebiology.biomedcentral.com/articles/10.1186/s13059-014-0550-8}, -volume = {15}, -year = {2014} -} - -@article{Rigby2023, -author = {Rigby, Rachel and Rue-Albrecht, Kevin and Sims, David and Rehwinkel, Jan}, -file = {:Users/fede/Downloads/2023.07.03.547491v1.full.pdf:pdf}, -journal = {bioRxiv}, -pages = {1--68}, -title = {{Single-cell analysis of signalling responses to type I interferons}}, -year = {2023} -} - diff --git a/vignettes/images/README.md b/vignettes/images/README.md deleted file mode 100644 index 1ca24a8..0000000 --- a/vignettes/images/README.md +++ /dev/null @@ -1 +0,0 @@ -This folder contains the image files used by the different vignette files diff --git a/vignettes/images/iSEE.png b/vignettes/images/iSEE.png deleted file mode 100644 index e496442..0000000 Binary files a/vignettes/images/iSEE.png and /dev/null differ diff --git a/vignettes/images/qrcode_iSEEdemo.png b/vignettes/images/qrcode_iSEEdemo.png deleted file mode 100644 index 976676b..0000000 Binary files a/vignettes/images/qrcode_iSEEdemo.png and /dev/null differ diff --git a/vignettes/images/qrcode_iSEEdemo_rendered.png b/vignettes/images/qrcode_iSEEdemo_rendered.png deleted file mode 100644 index d21db6a..0000000 Binary files a/vignettes/images/qrcode_iSEEdemo_rendered.png and /dev/null differ