params.txt

bowtie_mode=very-sensitive
bowtie_version=bowtie
CDS_only=1
CpG_exclusive=0
crispra_downstream=50
crispra_upstream=400
crispri_downstream=50
crispri_upstream=300
data_type=ensemble_acc
databasepath=./
downstream_window=50
edit_distance_allowed=1
exclude_overlapping_genes=0
exon_exclusive=1
gene_exclusive=1
ignore_intergenic=0
ignore_missing_id=0
kind=single
left_homology=500
max_A=90    
max_C=90
max_G=90
max_length=20        
max_per_exon=400
max_T=90
maxspacerlength=17
min_A=1
min_C=1    
min_G=1
min_length=20
min_T=1
minspacerlength=15
number_of_CDS=1
targets-allowed=10
offtargetdb=genomeDNA
out_gff=1
PAM_location=3_prime
PAM=NGG
preceding=N
purpose_exclusive=0
purpose=knockout
ref_organism=homo_sapiens
retrieve_recomb_matrix=0
right_homology=500
scores=doench_old
sec_off_target=0
sort_by_rank=0
specific_exon=any
specific_transcript=any
unspecific_leading_bases=4
upstream_window=50