diff --git a/lib/galaxy/datatypes/sequence.py b/lib/galaxy/datatypes/sequence.py
index 911a1a2db31a..71fbc0ec8d9c 100644
--- a/lib/galaxy/datatypes/sequence.py
+++ b/lib/galaxy/datatypes/sequence.py
@@ -774,7 +774,7 @@ def display_data(
     ):
         headers = kwd.get("headers", {})
         if preview:
-            with compression_utils.get_fileobj(dataset.get_file_name()) as fh:
+            with compression_utils.get_fileobj(dataset.get_file_name(), "rb") as fh:
                 max_peek_size = 1000000  # 1 MB
                 if os.stat(dataset.get_file_name()).st_size < max_peek_size:
                     mime = "text/plain"
diff --git a/lib/galaxy/datatypes/tabular.py b/lib/galaxy/datatypes/tabular.py
index 5b4e1d523e12..ea381d1f9c0b 100644
--- a/lib/galaxy/datatypes/tabular.py
+++ b/lib/galaxy/datatypes/tabular.py
@@ -154,12 +154,12 @@ def get_chunk(self, trans, dataset: HasFileName, offset: int = 0, ck_size: Optio
         )
 
     def _read_chunk(self, trans, dataset: HasFileName, offset: int, ck_size: Optional[int] = None):
-        with compression_utils.get_fileobj(dataset.get_file_name()) as f:
+        with compression_utils.get_fileobj(dataset.get_file_name(), "rb") as f:
             f.seek(offset)
             ck_data = f.read(ck_size or trans.app.config.display_chunk_size)
-            if ck_data and ck_data[-1] != "\n":
+            if ck_data and ck_data[-1] != b"\n":
                 cursor = f.read(1)
-                while cursor and cursor != "\n":
+                while cursor and cursor != b"\n":
                     ck_data += cursor
                     cursor = f.read(1)
             last_read = f.tell()