diff --git a/lib/galaxy/tool_util/linters/output.py b/lib/galaxy/tool_util/linters/output.py
index 37ebab7343d4..4068bf4d9d42 100644
--- a/lib/galaxy/tool_util/linters/output.py
+++ b/lib/galaxy/tool_util/linters/output.py
@@ -1,5 +1,6 @@
 """This module contains a linting functions for tool outputs."""
 
+import ast
 from typing import TYPE_CHECKING
 
 from packaging.version import Version
@@ -76,6 +77,24 @@ def lint(cls, tool_source: "ToolSource", lint_ctx: "LintContext"):
             names.add(name)
 
 
+class OutputsFilterExpression(Linter):
+    @classmethod
+    def lint(cls, tool_source: "ToolSource", lint_ctx: "LintContext"):
+        tool_xml = getattr(tool_source, "xml_tree", None)
+        if not tool_xml:
+            return
+        labels = set()
+        for filter in tool_xml.findall("./outputs//filter"):
+            try:
+                ast.parse(filter.text, mode="eval")
+            except Exception as e:
+                lint_ctx.error(
+                    f"Filter '{filter.text}' is no valid expression: {str(e)}",
+                    linter=cls.name(),
+                    node=filter,
+                )
+
+
 class OutputsLabelDuplicatedFilter(Linter):
     @classmethod
     def lint(cls, tool_source: "ToolSource", lint_ctx: "LintContext"):
diff --git a/test/unit/tool_util/test_tool_linters.py b/test/unit/tool_util/test_tool_linters.py
index 7e6d3b62ec93..c0aad892546b 100644
--- a/test/unit/tool_util/test_tool_linters.py
+++ b/test/unit/tool_util/test_tool_linters.py
@@ -638,15 +638,31 @@
         <data name="valid_name" format="fasta"/>
         <data name="valid_name" format="fasta"/>
         <data name="another_valid_name" format="fasta" label="same label may be OK if there is a filter">
-            <filter>a condition</filter>
+            <filter>a and condition</filter>
         </data>
         <data name="yet_another_valid_name" format="fasta" label="same label may be OK if there is a filter">
-            <filter>another condition</filter>
+            <filter>another or condition</filter>
         </data>
     </outputs>
 </tool>
 """
 
+# check if filters are valid python expressions
+OUTPUTS_FILTER_EXPRESSION = """
+<tool id="id" name="name">
+    <outputs>
+        <data name="another_valid_name" format="fasta" label="a label">
+            <filter>an invalid condition</filter>
+            <filter>an and condition</filter>
+        </data>
+        <collection name="yet_another_valid_name" type="list" format="fasta" label="another label">
+            <filter>another invalid condition</filter>
+            <filter>another or condition</filter>
+        </collection>
+    </outputs>
+</tool>
+"""
+
 # tool xml for repeats linter
 REPEATS = """
 <tool id="id" name="name">
@@ -1678,6 +1694,25 @@ def test_outputs_duplicated_name_label(lint_ctx):
     assert len(lint_ctx.error_messages) == 1
 
 
+def test_outputs_filter_expression(lint_ctx):
+    """ """
+    tool_source = get_xml_tool_source(OUTPUTS_FILTER_EXPRESSION)
+    run_lint_module(lint_ctx, output, tool_source)
+    assert "2 outputs found." in lint_ctx.info_messages
+    assert len(lint_ctx.info_messages) == 1
+    assert not lint_ctx.valid_messages
+    assert not lint_ctx.warn_messages
+    assert (
+        "Filter 'another invalid condition' is no valid expression: invalid syntax (<unknown>, line 1)"
+        in lint_ctx.error_messages
+    )
+    assert (
+        "Filter 'another invalid condition' is no valid expression: invalid syntax (<unknown>, line 1)"
+        in lint_ctx.error_messages
+    )
+    assert len(lint_ctx.error_messages) == 2
+
+
 def test_stdio_default_for_default_profile(lint_ctx):
     tool_source = get_xml_tool_source(STDIO_DEFAULT_FOR_DEFAULT_PROFILE)
     run_lint_module(lint_ctx, stdio, tool_source)
@@ -2145,7 +2180,7 @@ def test_skip_by_module(lint_ctx):
 def test_list_linters():
     linter_names = Linter.list_listers()
     # make sure to add/remove a test for new/removed linters if this number changes
-    assert len(linter_names) == 132
+    assert len(linter_names) == 133
     assert "Linter" not in linter_names
     # make sure that linters from all modules are available
     for prefix in [