From fc3dbebadd204a17ef5a50a13e655493f70f2898 Mon Sep 17 00:00:00 2001 From: "C. Titus Brown" Date: Tue, 6 Dec 2022 06:09:17 -0800 Subject: [PATCH] [MRG] add code to support skipping genomes by ident (#255) * add code to support skipping genomes by ident * add test * add 'skip_genomes' docs and tests * fix a few things --- doc/configuring.md | 10 +++ genome_grist/conf/Snakefile | 22 ++++-- .../GCF_000020205-is-missing.trim.sig.zip | Bin 0 -> 43881 bytes tests/test-data/GCF_000020205.1.sig.zip | Bin 0 -> 43881 bytes tests/test-data/conf-missing-skip.yml | 10 +++ tests/test-data/conf-missing.yml | 9 +++ tests/test_snakemake_errors.py | 70 ++++++++++++++++++ 7 files changed, 115 insertions(+), 6 deletions(-) create mode 100644 tests/test-data/GCF_000020205-is-missing.trim.sig.zip create mode 100644 tests/test-data/GCF_000020205.1.sig.zip create mode 100644 tests/test-data/conf-missing-skip.yml create mode 100644 tests/test-data/conf-missing.yml diff --git a/doc/configuring.md b/doc/configuring.md index 2900756..acf7201 100644 --- a/doc/configuring.md +++ b/doc/configuring.md @@ -346,6 +346,16 @@ prevent_sra_download: false # picklist: some_sig_list.csv:ident:ident picklist: "" +# skip_genomes: identifiers to ignore when they show up in gather output. +# This is useful when the sourmash database contains genomes that are no +# longer present in GenBank because they have been deprecated or suppressed. +# +# Note, in such cases you should try to find a new genome to include in +# a local database! +# +# DEFAULT: [] +skip_genomes: [] + # sourmash_database_threshold_bp: sets the --threshold-bp minimum match # size of sourmash prefetch and gather. Matches with smaller overlaps # than this will be excluded from consideration. diff --git a/genome_grist/conf/Snakefile b/genome_grist/conf/Snakefile index 276d198..c68921d 100755 --- a/genome_grist/conf/Snakefile +++ b/genome_grist/conf/Snakefile @@ -94,6 +94,8 @@ def make_param_str(ksizes, scaled): ks = ",".join(ks) return f"{ks},scaled={scaled},abund" +IGNORE_IDENTS = config.get('skip_genomes', []) + ### # # print out unknown config variables... @@ -113,6 +115,7 @@ known_config_keys = { 'samples', 'outdir', 'tempdir', 'prefetch_memory', 'taxonomies', 'prevent_sra_download', + 'skip_genomes', 'picklist' } unknown_config_keys = all_config_keys - known_config_keys @@ -262,6 +265,8 @@ class Checkpoint_GatherResults: genome_idents = [] for row in load_gather_csv(gather_csv): ident = row['name'].split()[0] + if ident in IGNORE_IDENTS: + continue genome_idents.append(ident) return genome_idents @@ -1047,7 +1052,8 @@ rule download_matching_genome_wc: input: csvfile = ancient(f'{GENBANK_CACHE}/{{ident}}.info.csv') output: - genome = f"{GENBANK_CACHE}/{{ident}}_genomic.fna.gz" + genome = f"{GENBANK_CACHE}/{{ident}}_genomic.fna.gz", + retries: 2 run: rows = list(load_csv(input.csvfile)) assert len(rows) == 1 @@ -1060,11 +1066,15 @@ rule download_matching_genome_wc: print(f"downloading genome for ident {ident}/{name} from NCBI...", file=sys.stderr) with open(output.genome, 'wb') as outfp: - with urllib.request.urlopen(url) as response: - content = response.read() - outfp.write(content) - print(f"...wrote {len(content)} bytes to {output.genome}", - file=sys.stderr) + try: + with urllib.request.urlopen(url) as response: + content = response.read() + outfp.write(content) + print(f"...wrote {len(content)} bytes to {output.genome}", + file=sys.stderr) + except urllib.request.HTTPError: + print(f"Cannot download genome from URL:\n {url}\nIs it missing? If so, consider adding '{ident}' to 'skip_genomes' list in config file.", file=sys.stderr) + raise Exception("Genbank genome not found") # summarize_reads_info rule summarize_reads_info_wc: diff --git a/tests/test-data/GCF_000020205-is-missing.trim.sig.zip b/tests/test-data/GCF_000020205-is-missing.trim.sig.zip new file mode 100644 index 0000000000000000000000000000000000000000..141a601fecb6e17e84e36fe1a49d11a6a058430f GIT binary patch literal 43881 zcmV)BK*PUKO9KQH000000HR}rRe#eni$AFV06(b!05Sjo0CQ<)ZeetFa%FQbI5ab6 zG%++YHDonpFkv=kW??mCF=91gI51;nIbt|uE^}#TE@yfliwFP!00004|1`PFj&)s* z9QG=W8Ye8?B7$td14Dof81Td%dLR^}l2uT5$xw9*hW+x6wewegc{!Vl3wo>{KmYwd{ky;a_5b?Ypa11w|Lb4=^z)~`{PUmx z`cGc;%m4J&{^P&??ce{)U;oSBY>NNkpB?b8|CQs#|M=H``pe(`{Lg=}{onkDzx=m< z^B@1izhT?{hwXp)xBv6M3x8Eq;D5+}_}~BhPk;ZH|MIuL{`nvN@4x>0|NNJK`Io=` z^&hhS*MIo?zx>xf|MhSGqhJ2~!~gVu{BO_wzolXR>97C(FMs>{L;tt`Hhlf}|4#Am zfBHw4@V9^c=Rf^p{QT)Z|NXE3ubn!7{`B|1{N+FV=^v+l{`Aj({(t`6e=_$!{bTM7 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