diff --git a/packages/react/__tests__/__snapshots__/PublicAPI-test.js.snap b/packages/react/__tests__/__snapshots__/PublicAPI-test.js.snap
index 4ad728b59e2b..6ce59ab366c0 100644
--- a/packages/react/__tests__/__snapshots__/PublicAPI-test.js.snap
+++ b/packages/react/__tests__/__snapshots__/PublicAPI-test.js.snap
@@ -6251,6 +6251,55 @@ Map {
       "autoAlign": Object {
         "type": "bool",
       },
+      "autoAlignBoundary": Object {
+        "args": Array [
+          Array [
+            Object {
+              "args": Array [
+                Array [
+                  "clippingAncestors",
+                ],
+              ],
+              "type": "oneOf",
+            },
+            Object {
+              "type": "elementType",
+            },
+            Object {
+              "args": Array [
+                Object {
+                  "type": "elementType",
+                },
+              ],
+              "type": "arrayOf",
+            },
+            Object {
+              "args": Array [
+                Object {
+                  "height": Object {
+                    "isRequired": true,
+                    "type": "number",
+                  },
+                  "width": Object {
+                    "isRequired": true,
+                    "type": "number",
+                  },
+                  "x": Object {
+                    "isRequired": true,
+                    "type": "number",
+                  },
+                  "y": Object {
+                    "isRequired": true,
+                    "type": "number",
+                  },
+                },
+              ],
+              "type": "exact",
+            },
+          ],
+        ],
+        "type": "oneOfType",
+      },
       "caret": Object {
         "type": "bool",
       },
diff --git a/packages/react/src/components/Popover/Popover.stories.js b/packages/react/src/components/Popover/Popover.stories.js
index 7007f18b6678..95f41d398bc6 100644
--- a/packages/react/src/components/Popover/Popover.stories.js
+++ b/packages/react/src/components/Popover/Popover.stories.js
@@ -270,6 +270,136 @@ export const ExperimentalAutoAlign = () => {
   );
 };
 
+export const ExperimentalAutoAlignBoundary = () => {
+  const [open, setOpen] = useState(true);
+  const ref = useRef();
+  const [boundary, setBoundary] = useState();
+
+  useEffect(() => {
+    ref?.current?.scrollIntoView({ block: 'center', inline: 'center' });
+  });
+
+  return (
+    <div
+      style={{
+        display: 'grid',
+        placeItems: 'center',
+        overflow: 'scroll',
+        width: '800px',
+        height: '500px',
+        border: '1px',
+        borderStyle: 'dashed',
+        borderColor: 'black',
+        margin: '0 auto',
+      }}
+      ref={setBoundary}>
+      <div
+        style={{
+          width: '2100px',
+          height: '1px',
+          placeItems: 'center',
+        }}
+      />
+      <div style={{ placeItems: 'center', height: '32px', width: '32px' }}>
+        <Popover
+          open={open}
+          align="top"
+          autoAlign
+          autoAlignBoundary={boundary}
+          ref={ref}>
+          <div className="playground-trigger">
+            <CheckboxIcon
+              onClick={() => {
+                setOpen(!open);
+              }}
+            />
+          </div>
+          <PopoverContent className="p-3">
+            <div>
+              <p className="popover-title">This popover uses autoAlign</p>
+              <p className="popover-details">
+                Scroll the container up, down, left or right to observe how the
+                popover will automatically change its position in attempt to
+                stay within the viewport. This works on initial render in
+                addition to on scroll.
+              </p>
+            </div>
+          </PopoverContent>
+        </Popover>
+        <div
+          style={{
+            height: '1000px',
+            width: '1px',
+            placeItems: 'center',
+          }}
+        />
+      </div>
+    </div>
+  );
+};
+
+export const Test = () => {
+  const [open, setOpen] = useState();
+  const align = document?.dir === 'rtl' ? 'bottom-right' : 'bottom-left';
+  const alignTwo = document?.dir === 'rtl' ? 'bottom-left' : 'bottom-right';
+  return (
+    <div style={{ display: 'flex', gap: '8rem' }}>
+      <OverflowMenu
+        flipped={document?.dir === 'rtl'}
+        aria-label="overflow-menu">
+        <OverflowMenuItem itemText="Stop app" />
+        <OverflowMenuItem itemText="Restart app" />
+        <OverflowMenuItem itemText="Rename app" />
+        <OverflowMenuItem itemText="Clone and move app" disabled requireTitle />
+        <OverflowMenuItem itemText="Edit routes and access" requireTitle />
+        <OverflowMenuItem hasDivider isDelete itemText="Delete app" />
+      </OverflowMenu>
+
+      <Popover
+        align={align}
+        open={open}
+        onKeyDown={(evt) => {
+          if (match(evt, keys.Escape)) {
+            setOpen(false);
+          }
+        }}
+        isTabTip
+        onRequestClose={() => setOpen(false)}>
+        <button
+          aria-label="Settings"
+          type="button"
+          aria-expanded={open}
+          onClick={() => {
+            setOpen(!open);
+          }}>
+          <Settings />
+        </button>
+        <PopoverContent className="p-3">
+          <RadioButtonGroup
+            style={{ alignItems: 'flex-start', flexDirection: 'column' }}
+            legendText="Row height"
+            name="radio-button-group"
+            defaultSelected="small">
+            <RadioButton labelText="Small" value="small" id="radio-small" />
+            <RadioButton labelText="Large" value="large" id="radio-large" />
+          </RadioButtonGroup>
+          <hr />
+          <fieldset className={`cds--fieldset`}>
+            <legend className={`cds--label`}>Edit columns</legend>
+            <Checkbox defaultChecked labelText="Name" id="checkbox-label-1" />
+            <Checkbox defaultChecked labelText="Type" id="checkbox-label-2" />
+            <Checkbox
+              defaultChecked
+              labelText="Location"
+              id="checkbox-label-3"
+            />
+          </fieldset>
+        </PopoverContent>
+      </Popover>
+    </div>
+  );
+};
+
 export const TabTipExperimentalAutoAlign = () => {
   const [open, setOpen] = useState(true);
   const ref = useRef();
diff --git a/packages/react/src/components/Popover/index.tsx b/packages/react/src/components/Popover/index.tsx
index 44f5061555d3..36e59ea3c1a3 100644
--- a/packages/react/src/components/Popover/index.tsx
+++ b/packages/react/src/components/Popover/index.tsx
@@ -28,6 +28,7 @@ import {
   autoUpdate,
   arrow,
   offset,
+  type Boundary,
 } from '@floating-ui/react';
 import { hide } from '@floating-ui/dom';
 import { useFeatureFlag } from '../FeatureFlags';
@@ -102,6 +103,11 @@ interface PopoverBaseProps {
    */
   autoAlign?: boolean;
 
+  /**
+   * Specify a bounding element to be used for autoAlign calculations. The viewport is used by default. This prop is currently experimental and is subject to future changes.
+   */
+  autoAlignBoundary?: Boundary;
+
   /**
    * Specify whether a caret should be rendered
    */
@@ -165,6 +171,7 @@ export const Popover: PopoverComponent = React.forwardRef(
       align: initialAlign = isTabTip ? 'bottom-start' : 'bottom',
       as: BaseComponent = 'span' as E,
       autoAlign = false,
+      autoAlignBoundary,
       caret = isTabTip ? false : true,
       className: customClassName,
       children,
@@ -292,6 +299,7 @@ export const Popover: PopoverComponent = React.forwardRef(
 
                   fallbackStrategy: 'initialPlacement',
                   fallbackAxisSideDirection: 'start',
+                  boundary: autoAlignBoundary,
                 }),
               arrow({
                 element: caretRef,
@@ -476,7 +484,7 @@ export const Popover: PopoverComponent = React.forwardRef(
       </PopoverContext.Provider>
     );
   }
-);
+) as PopoverComponent;
 
 // Note: this displayName is temporarily set so that Storybook ArgTable
 // correctly displays the name of this component
@@ -546,6 +554,21 @@ Popover.propTypes = {
    */
   autoAlign: PropTypes.bool,
 
+  /**
+   * Specify a bounding element to be used for autoAlign calculations. The viewport is used by default. This prop is currently experimental and is subject to future changes.
+   */
+  autoAlignBoundary: PropTypes.oneOfType([
+    PropTypes.oneOf(['clippingAncestors']),
+    PropTypes.elementType,
+    PropTypes.arrayOf(PropTypes.elementType),
+    PropTypes.exact({
+      x: PropTypes.number.isRequired,
+      y: PropTypes.number.isRequired,
+      width: PropTypes.number.isRequired,
+      height: PropTypes.number.isRequired,
+    }),
+  ]) as PropTypes.Validator<Boundary | null | undefined>,
+
   /**
    * Specify whether a caret should be rendered
    */