| 0 |
Name |
target site ID |
| 1 |
Length |
target length |
| 2 |
Chromosome |
target chromosome |
| 3 |
Start |
target start relative to the chromosome |
| 4 |
End |
target end relative to the chromosome |
| 5 |
Strand |
strand it will target |
| 6 |
Nucleotide sequence |
target site nucleotide composition of the form target_PAM |
| 7 |
Gene Name |
ID::GENE::STRAND |
| 8 |
Transcripts |
ENSEMBL transcript Ids overlapping with the target site "_"-separated |
| 9 |
Transcript:: Exon |
ENSEMBL transcript::exon Ids overlapping with the target site |
| 10 |
Number of Cpg Islands hit |
Number of Cpg Islands overlapping with the target site |
| 11 |
Sequence around the cutside |
if it is chosen to save a recombination matrix ist sequence is here |
| 12 |
%A %C %T %G |
nucleotide compositions in per cent |
| 13 |
S-Score |
specificity score |
| 14 |
A-Score |
annotation score |
| 15 |
Custom-Score |
by default the score from Doench et al. 2014 else the score deviated from the custom Perl scoring script |
| 16 |
Doench-Score |
Efficacy score as introduced by Doench et al. 2014 Nat. Biotech. |
| 17 |
Xu-Score |
Efficacy score as introduced by Xu et al. 2015 Gen.Res. |
| 18 |
percent of total transcripts hit |
per cent of transcripts of the targeted gene being hit by that putative sgRNA |
| 19 |
Match-Target |
target genes found by remapping the target site |
| 20 |
Match-Chromosome |
target chromosome found by remapping the target site |
| 21 |
Match-Start |
alignment start with respect to the estimate target chromosome |
| 22 |
Match-End |
alignment end with respect to the estimate target chromosome |
| 23 |
Matchstring |
alignment representation "M" for match "X" for mismatch "I" for insertion "D" for deletion |
| 24 |
Editdistance |
estimated edit distance of the alignment (X+I+D, sum of all deletions, insertions or exchanges neccessary to edit the matched sequence to the target sequence) |
| 25 |
Number of Hits |
estimated number of target sites in the respective genome with the off-target parameters specified |
| 26 |
Direction |
strandedness of the target alignment |
| 27 |
Start_rti |
target start with respect to the estimate target gene |
| 28 |
End_rti |
target end with respect to the estimate target gene |