Changes:
| version | modifications |
|---|---|
| v1.1.0 | + added strandedness information for single exon genes |
| + fixed GUI rules for CRISPRa/i | |
| + added option so TSS are only counted if belonging to the gene of interest | |
| + changed bowtie off-target mode to -k ( offtargets allowed +1 ) so that bowtie always searches if designs have less or at least on off-target more than allowed. This way, sgRNAs are excluded which do have more than the allowed off-targets and included if less. Earlier one could allow more off-targets then bowtie was searching for. | |
| v1.2.0 | + fixed overlapping criterion to beeing 3 bp downstream of the PAM (sgRNAend - 5bp) |
| v1.3.0 | + fixed error in version 1.2 where filter criteria like exons were not interpreted |
| + enabled automated deletion of single gene files in the server appliation so only the usmmary is kept | |
| + fixed all binary builds | |
| v1.3.1 | + fixed error in version 1.3.0 where gene names with underscores caused major trouble |
| v1.4.0 | + removed html output option |
| + fixed alignment match coordinates to be according to unspecific leading base pairs | |
| + added the columns, Chromosome, Match-Chromosome, Start_rti, End_rti (relative to input) | |
| + fixed Manual and Readme for the new cloumn headings | |
| + fixed sgRNA ranking accordingly | |
| v1.4.1 | + fixed a problem when purpose was set to non-coding so that not all possible sgRNA were considered for the library fitering |
| v1.4.3 | + Added TTTV as PAM |
| + fixed output fasta file when PAM is 5' | |
| v1.4.4 | + fixed 5_prime PAM matchstring |
| + fixed 5_prime PAM off-target behaviour | |
| + changed off-targets-allowed parameter to targets-allowed the number has to be greater than 1. Else all sgRNAs are excluded also those that only have one "on-target" | |
| v1.4.5 | + fixed error handling with doubled gene ids. Now a warning is thrown and genes are processed in alphanumerical order |
| + fixed issues when Genes had similar names with numerics e.g. RPL3 and RPL30 | |
| + fixed counting of missing genes | |
| v1.4.6 | + fixed an issue with library output in fasta format, where only the PAM was in the output oligo |