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Feature Request (Background RNA Counts in RowData) #26

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sanjayrsrivatsan opened this issue Feb 23, 2021 · 0 comments
Open

Feature Request (Background RNA Counts in RowData) #26

sanjayrsrivatsan opened this issue Feb 23, 2021 · 0 comments

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@sanjayrsrivatsan
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I want to request a feature that adds ambient RNA counts per gene to the rowData of the output CDS object.

I am imaging that a minimum UMI cutoff can be used to define background RNA and gene expression counts from these ambient RNA molecules can be summed to create a vector of background gene expression counts, and finally added to the rowData of the CDS.

For instance, if the UMI cutoff is set to 1 UMI, all of the counts from "cells" that have 1 UMI are summed across all genes to define a background RNA set.

@hpliner hpliner transferred this issue from bbi-lab/bbi-dmux Feb 25, 2021
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